pycom12g04520

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000234
Physical Location & Seq
Forward (+)
4385429 .. 4386128
700 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04520.1

Sequence Viewer

Length: 648 bp
ATGTTTAGTCACAATATACCCAAAGAAATCTTGTTTGATATCCTTGCAAGATTGCCAGTGAGATCTCTTTTGCGGTTTAGATGCGTTTGTAAATCTTGGAAAAGTTTAATTAGCAGCTCTAGTTTCGAAAGTGCCCATCTCGAAAGGAACGTCATGCAAAGTTCTTGTGATTTTCTCCTTATCCAAACAGATGACAAGAAAAATTGTTTGTCACTGTTTGATGCTGAAACATTGTCCAAGTGTTTGGACATAGAGCTTCCTTCGGATAAATTAGGTCTCAGATTCTTTGTTCATGGTTCGTGCAAAGGATTTGTTTGTATCTCTTTTTATAATTTCTTTAGTTTGAATAATAATCCTATATATCTATGGAATCCCTCAATCAGAAAACTCAAGAGGCTTCCTAATGGCCTCGTTCAGCACACAAGCCATTTTGTTTTGCTTCCACGTGAGAATGTCGTCCTTGAAGTTGAGGTTTACAGCCTTAGATCAGATGCTTGGAGAAGAATAATTGTGATGCATGTTGGAAACAACACCAATACTGACCCCAACTTTACCACCTGCCCAATCCGTCAATCAGAAAACTCAAAAGGCTTCCTAATGGCCTCGTTCAGCACACAAGCCATTTTGTTAATGGTTTTGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

216

Amino Acids

24.67

Weight (kDa)

8.96

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 42 2.9e-11 F-box domain
F-box-like PF12937 5 - 40 8.9e-10 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AarI CACCTGC 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 566
AciI CCGC 1 cut(s) 73
AcvI CACGTG 1 cut(s) 446
AgsI TTSAA 2 cut(s) 346, 464
AluBI AGCT 2 cut(s) 117, 256
AluI AGCT 2 cut(s) 117, 256
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 2 cut(s) 406, 600
ApeKI GCWGC 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AsuII TTCGAA 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 136
BbrPI CACGTG 1 cut(s) 446
BbvI GCAGC 1 cut(s) 126
BccI CCATC 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 566
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
BmsI GCATC 4 cut(s) 71, 211, 481, 504
Bpu14I TTCGAA 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 1 cut(s) 126
BshFI GGCC 2 cut(s) 408, 602
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 2 cut(s) 408, 602
Bso31I GGTCTC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 62, 485
BspACI CCGC 1 cut(s) 73
BspANI GGCC 2 cut(s) 408, 602
BspCNI CTCAG 1 cut(s) 291
BspMI ACCTGC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 56
BssMI GATC 2 cut(s) 62, 485
Bst4CI ACNGT 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 278, 482
BstKTI GATC 2 cut(s) 65, 488
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 2 cut(s) 62, 485
BstNSI RCATGY 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 2 cut(s) 408, 602
BtsIMutI CAGTG 2 cut(s) 63, 212
BveI ACCTGC 1 cut(s) 566
CviAII CATG 3 cut(s) 154, 293, 518
CviJI RGCY 9 cut(s) 117, 256, 397, 408, 426, 480, 591, 602, 620
CviKI_1 RGCY 9 cut(s) 117, 256, 397, 408, 426, 480, 591, 602, 620
DdeI CTNAG 2 cut(s) 278, 482
DpnI GATC 2 cut(s) 64, 487
DpnII GATC 2 cut(s) 62, 485
Eco31I GGTCTC 1 cut(s) 281
Eco32I GATATC 1 cut(s) 40
Eco72I CACGTG 1 cut(s) 446
EcoRV GATATC 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 519
FaeI CATG 3 cut(s) 157, 296, 521
FatI CATG 3 cut(s) 153, 292, 517
Fnu4HI GCNGC 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 120
GluI GCNGC 1 cut(s) 115
HaeIII GGCC 2 cut(s) 408, 602
Hin1II CATG 3 cut(s) 157, 296, 521
HinfI GANTC 2 cut(s) 282, 370
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 5 cut(s) 265, 281, 383, 490, 577
Hpy188III TCNNGA 2 cut(s) 140, 391
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 1 cut(s) 270
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 2 cut(s) 150, 445
HpyCH4V TGCA 4 cut(s) 47, 157, 303, 517
HpyF3I CTNAG 2 cut(s) 278, 482
HpySE526I ACGT 2 cut(s) 150, 445
Hsp92II CATG 3 cut(s) 157, 296, 521
Kzo9I GATC 2 cut(s) 62, 485
LpnPI CCDG 2 cut(s) 69, 571
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 4 cut(s) 71, 211, 481, 504
MaeI CTAG 1 cut(s) 120
MaeII ACGT 2 cut(s) 150, 445
MaeIII GTNAC 2 cut(s) 8, 210
MalI GATC 2 cut(s) 64, 487
MboI GATC 2 cut(s) 62, 485
MboII GAAGA 1 cut(s) 513
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 5 cut(s) 108, 202, 269, 331, 507
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 5 cut(s) 385, 387, 419, 463, 613
Mph1103I ATGCAT 1 cut(s) 519
MseI TTAA 2 cut(s) 107, 629
NdeII GATC 2 cut(s) 62, 485
NlaIII CATG 3 cut(s) 157, 296, 521
NmuCI GTSAC 2 cut(s) 8, 210
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 521
NspV TTCGAA 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 566
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 2 cut(s) 282, 370
PkrI GCNGC 1 cut(s) 116
PmaCI CACGTG 1 cut(s) 446
PmlI CACGTG 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 446
PsiI TTATAA 1 cut(s) 330
PspCI CACGTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 62
SaqAI TTAA 2 cut(s) 107, 629
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 2 cut(s) 62, 485
SduI GDGCHC 1 cut(s) 136
SetI ASST 7 cut(s) 119, 153, 258, 277, 448, 474, 560
SfaNI GCATC 4 cut(s) 71, 211, 481, 504
SfuI TTCGAA 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 5 cut(s) 108, 202, 269, 331, 507
SsiI CCGC 1 cut(s) 73
SspMI CTAG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 2 cut(s) 153, 448
TaqI TCGA 2 cut(s) 126, 141
TasI AATT 5 cut(s) 108, 202, 269, 331, 507
TfiI GAWTC 2 cut(s) 282, 370
Tru1I TTAA 2 cut(s) 107, 629
Tru9I TTAA 2 cut(s) 107, 629
TscAI CASTG 2 cut(s) 63, 219
TseFI GTSAC 2 cut(s) 8, 210
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 2 cut(s) 8, 210
TspDTI ATGAA 2 cut(s) 281, 633
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 2 cut(s) 63, 219
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.