Rh6DG445500

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000234
Physical Location & Seq
Forward (+)
61970272 .. 61972411
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG445500.1

Sequence Viewer

Length: 1107 bp
ATGTCTACCATCGAAGACCTACCTGCAGAAATATTGTTTGATATCTTTGCAAGATTACCTGTGAAATCTGCTTTCCTACTAAGATGGGTTTGCAAATCTTTCAAGACTTTAATTACAAGTTCTGATTTCATACACTTCCATCTCGAAAGGAATCCCATGAAAAATTCTTCCGATTATCTACTTATCCGCAGTACGGAGATTGATTGCATGTCACGCATTTGTGCTAGAACATTTGCTAGGGATTTGGATATAAAGCTTCCTGAGAATGCTGTGTACGAGTTGGGTTCTGAATTAACTGTTTATGGTTCATACAATGGGTTGCTTTGCATATCCAACAGGTATCTGTGTGTAGATAGCCCTATATACTTGTGGAATCCATCAATTAGAAAAATCAGAAGACTTCCCTATGGCCTCATCACAAGCACCATTAATGACCACGATTATCAAGTTACTCTTGGGATGGGGTTCCATTCTGCAGGTGGGAATGACTATAAGGTTATAAAGTTTGTGCACCATGATACTGACTCTGAAAATACTTTTCAAGTTGAGGTATACAGTCTTAATTCAAATTCTTGGAAAAGAATAAGCAGAGTTCCCCCTGTTCCACGAGATGCTTATTTTCGCCCGAAATGTGCATATTTGAATGGAGTTGTGTATTGGATTCTATCTGAAGACCTCTTGTCCATCATTTCTTTTGATGTGCACAACGAGTCATTTCGAACCAGGAGCTATAGTAAGGACTTGTTCCCACTTATGCATCCAAACTGTATGCAAGTGTGGAACAATTCACTTTGTTTGTTTCGGCCCAAAGTCGCTGAATATGATGGGAAGAATACAAGCTGCTGTGAGATATACATTCTGAACATGGTAACAAGTAAATTGGAACTATCCAGGACTCTTCGTATGGATAGTTTTGTATCTATAGCATGGCCATTGGGGTTCAACACAAGTGGCAGTGGGAAAGACATTGATATGGTTATCAGGACTGATAGTCGACCACCAATACTTGTTTCATGTACTCCTCAAGGGCACGAAATTGAACTAGGTATGTTCGAAGTTTGCTGGTACGTTGATGTTTATAGAGAGAGTCTGCTTCTACTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

368

Amino Acids

42.45

Weight (kDa)

6.16

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 44 1.3e-07 F-box domain
F-box-like PF12937 4 - 41 3.3e-06 F-box-like
FBA_3 PF08268 96 - 250 9.3e-14 F-box associated beta propeller domain
FBA_1 PF07734 103 - 243 1.5e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 2 cut(s) 31, 467
AccI GTMKAC 3 cut(s) 5, 552, 994
AciI CCGC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 2 cut(s) 163, 568
AcuI CTGAAG 1 cut(s) 690
AfaI GTAC 4 cut(s) 193, 275, 1018, 1067
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 6 cut(s) 103, 542, 567, 643, 943, 1040
AhdI GACNNNNNGTC 2 cut(s) 679, 990
AjnI CCWGG 2 cut(s) 722, 890
AjuI GAANNNNNNNTTGG 2 cut(s) 994, 1026
AluBI AGCT 3 cut(s) 256, 729, 840
AluI AGCT 3 cut(s) 256, 729, 840
Alw21I GWGCWC 2 cut(s) 513, 705
Alw44I GTGCAC 2 cut(s) 509, 701
AoxI GGCC 3 cut(s) 409, 803, 929
ApaLI GTGCAC 2 cut(s) 509, 701
ApeKI GCWGC 1 cut(s) 840
ApoI RAATTY 2 cut(s) 163, 568
AseI ATTAAT 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 804
AsuII TTCGAA 2 cut(s) 718, 1053
BaeGI GKGCMC 3 cut(s) 513, 705, 1032
BalI TGGCCA 1 cut(s) 931
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 3 cut(s) 21, 403, 678
Bbv12I GWGCWC 2 cut(s) 513, 705
BbvI GCAGC 1 cut(s) 827
BccI CCATC 7 cut(s) 17, 78, 147, 385, 454, 692, 818
BciT130I CCWGG 2 cut(s) 724, 892
BfaI CTAG 3 cut(s) 225, 237, 1043
BfmI CTRYAG 4 cut(s) 24, 474, 730, 921
BfuAI ACCTGC 2 cut(s) 31, 467
BisI GCNGC 1 cut(s) 841
BlsI GCNGC 1 cut(s) 842
Bme1390I CCNGG 2 cut(s) 724, 892
BmeRI GACNNNNNGTC 2 cut(s) 679, 990
BmgT120I GGNCC 1 cut(s) 804
BmiI GGNNCC 1 cut(s) 467
BmrFI CCNGG 2 cut(s) 724, 892
BmsI GCATC 2 cut(s) 601, 766
BpiI GAAGAC 3 cut(s) 21, 403, 678
Bpu14I TTCGAA 2 cut(s) 718, 1053
BpuEI CTTGAG 1 cut(s) 1008
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 1 cut(s) 193
BseBI CCWGG 2 cut(s) 724, 892
BseGI GGATG 2 cut(s) 465, 757
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 1011
BseSI GKGCMC 3 cut(s) 513, 705, 1032
BseXI GCAGC 1 cut(s) 827
BshFI GGCC 3 cut(s) 411, 805, 931
BsiHKAI GWGCWC 2 cut(s) 513, 705
BslI CCNNNNNNNGG 1 cut(s) 193
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 3 cut(s) 411, 805, 931
Bsp119I TTCGAA 2 cut(s) 718, 1053
Bsp1286I GDGCHC 3 cut(s) 513, 705, 1032
BspACI CCGC 1 cut(s) 187
BspANI GGCC 3 cut(s) 411, 805, 931
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 467
BspMAI CTGCAG 2 cut(s) 28, 478
BspMI ACCTGC 2 cut(s) 31, 467
BspT104I TTCGAA 2 cut(s) 718, 1053
BssNAI GTATAC 1 cut(s) 553
BssSI CACGAG 1 cut(s) 606
Bst1107I GTATAC 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 2 cut(s) 724, 892
Bst4CI ACNGT 3 cut(s) 298, 557, 767
Bst6I CTCTTC 1 cut(s) 903
BstBI TTCGAA 2 cut(s) 718, 1053
BstDEI CTNAG 2 cut(s) 80, 261
BstF5I GGATG 2 cut(s) 465, 757
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstNI CCWGG 2 cut(s) 724, 892
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 722, 890
BstSFI CTRYAG 4 cut(s) 24, 474, 730, 921
BstSLI GKGCMC 3 cut(s) 513, 705, 1032
BstV1I GCAGC 1 cut(s) 827
BstV2I GAAGAC 3 cut(s) 21, 403, 678
BstZ17I GTATAC 1 cut(s) 553
BsuRI GGCC 3 cut(s) 411, 805, 931
BtsCI GGATG 2 cut(s) 465, 757
BtsI GCAGTG 1 cut(s) 961
BtsIMutI CAGTG 1 cut(s) 961
BveI ACCTGC 2 cut(s) 31, 467
Cfr13I GGNCC 1 cut(s) 804
Csp6I GTAC 4 cut(s) 192, 274, 1017, 1066
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 6 cut(s) 157, 208, 515, 865, 927, 1014
CviJI RGCY 7 cut(s) 256, 357, 411, 729, 805, 840, 931
CviKI_1 RGCY 7 cut(s) 256, 357, 411, 729, 805, 840, 931
CviQI GTAC 4 cut(s) 192, 274, 1017, 1066
DdeI CTNAG 2 cut(s) 80, 261
DriI GACNNNNNGTC 2 cut(s) 679, 990
EaeI YGGCCR 1 cut(s) 929
Eam1104I CTCTTC 1 cut(s) 903
Eam1105I GACNNNNNGTC 2 cut(s) 679, 990
EarI CTCTTC 1 cut(s) 903
Eco32I GATATC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 690
EcoRII CCWGG 2 cut(s) 722, 890
EcoRV GATATC 1 cut(s) 43
EcoT22I ATGCAT 1 cut(s) 759
FaeI CATG 6 cut(s) 160, 211, 518, 868, 930, 1017
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 6 cut(s) 156, 207, 514, 864, 926, 1013
FblI GTMKAC 3 cut(s) 5, 552, 994
Fnu4HI GCNGC 1 cut(s) 841
FokI GGATG 2 cut(s) 472, 744
Fsp4HI GCNGC 1 cut(s) 841
FspBI CTAG 3 cut(s) 225, 237, 1043
GluI GCNGC 1 cut(s) 841
HaeIII GGCC 3 cut(s) 411, 805, 931
Hin1II CATG 6 cut(s) 160, 211, 518, 868, 930, 1017
HincII GTYRAC 1 cut(s) 995
HindII GTYRAC 1 cut(s) 995
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 7 cut(s) 151, 373, 524, 661, 710, 895, 1087
Hpy166II GTNNAC 6 cut(s) 6, 274, 511, 553, 703, 995
Hpy188I TCNGA 7 cut(s) 124, 172, 289, 395, 529, 670, 861
Hpy188III TCNNGA 4 cut(s) 103, 143, 260, 982
Hpy8I GTNNAC 6 cut(s) 6, 274, 511, 553, 703, 995
HpyCH4III ACNGT 3 cut(s) 298, 557, 767
HpyCH4IV ACGT 1 cut(s) 1068
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 80, 261
HpySE526I ACGT 1 cut(s) 1068
Hsp92II CATG 6 cut(s) 160, 211, 518, 868, 930, 1017
LmnI GCTCC 1 cut(s) 726
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 2 cut(s) 601, 766
MaeI CTAG 3 cut(s) 225, 237, 1043
MaeII ACGT 1 cut(s) 1068
MaeIII GTNAC 3 cut(s) 210, 448, 868
MboII GAAGA 6 cut(s) 26, 159, 408, 683, 841, 890
MhlI GDGCHC 3 cut(s) 513, 705, 1032
MlsI TGGCCA 1 cut(s) 931
MluCI AATT 9 cut(s) 111, 163, 290, 381, 562, 568, 784, 878, 1035
MluNI TGGCCA 1 cut(s) 931
MlyI GAGTC 4 cut(s) 518, 719, 889, 1096
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 4 cut(s) 422, 541, 686, 1032
Mox20I TGGCCA 1 cut(s) 931
Mph1103I ATGCAT 1 cut(s) 759
MscI TGGCCA 1 cut(s) 931
MseI TTAA 4 cut(s) 110, 293, 429, 561
MslI CAYNNNNRTG 1 cut(s) 971
Msp20I TGGCCA 1 cut(s) 931
MspR9I CCNGG 2 cut(s) 724, 892
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 2 cut(s) 724, 892
MwoI GCNNNNNNNGC 1 cut(s) 213
NlaIII CATG 6 cut(s) 160, 211, 518, 868, 930, 1017
NlaIV GGNNCC 1 cut(s) 467
NmuCI GTSAC 1 cut(s) 210
NsiI ATGCAT 1 cut(s) 759
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 2 cut(s) 718, 1053
PaqCI CACCTGC 1 cut(s) 467
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 3 cut(s) 151, 373, 661
PfoI TCCNGGA 1 cut(s) 890
PkrI GCNGC 1 cut(s) 842
PleI GAGTC 4 cut(s) 518, 718, 889, 1095
PpsI GAGTC 4 cut(s) 518, 718, 889, 1095
PshBI ATTAAT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 722, 890
PspGI CCWGG 2 cut(s) 722, 890
PspN4I GGNNCC 1 cut(s) 467
PspPI GGNCC 1 cut(s) 804
PstI CTGCAG 2 cut(s) 28, 478
RsaI GTAC 4 cut(s) 193, 275, 1018, 1067
RsaNI GTAC 4 cut(s) 192, 274, 1017, 1066
RseI CAYNNNNRTG 1 cut(s) 971
SalI GTCGAC 1 cut(s) 993
SaqAI TTAA 4 cut(s) 110, 293, 429, 561
SatI GCNGC 1 cut(s) 841
Sau96I GGNCC 1 cut(s) 804
SchI GAGTC 4 cut(s) 518, 719, 889, 1096
ScrFI CCNGG 2 cut(s) 724, 892
SduI GDGCHC 3 cut(s) 513, 705, 1032
SfaNI GCATC 2 cut(s) 601, 766
SfcI CTRYAG 4 cut(s) 24, 474, 730, 921
SfuI TTCGAA 2 cut(s) 718, 1053
SmiMI CAYNNNNRTG 1 cut(s) 971
SmlI CTYRAG 1 cut(s) 1023
SmoI CTYRAG 1 cut(s) 1023
Sse9I AATT 9 cut(s) 111, 163, 290, 381, 562, 568, 784, 878, 1035
SsiI CCGC 1 cut(s) 187
SspI AATATT 1 cut(s) 33
SspMI CTAG 3 cut(s) 225, 237, 1043
StyD4I CCNGG 2 cut(s) 722, 890
TaaI ACNGT 3 cut(s) 298, 557, 767
TaiI ACGT 1 cut(s) 1071
TaqI TCGA 6 cut(s) 12, 144, 718, 994, 1053, 1101
TasI AATT 9 cut(s) 111, 163, 290, 381, 562, 568, 784, 878, 1035
TatI WGTACW 1 cut(s) 1016
TfiI GAWTC 3 cut(s) 151, 373, 661
Tru1I TTAA 4 cut(s) 110, 293, 429, 561
Tru9I TTAA 4 cut(s) 110, 293, 429, 561
TscAI CASTG 1 cut(s) 961
TseFI GTSAC 1 cut(s) 210
TseI GCWGC 1 cut(s) 840
Tsp45I GTSAC 1 cut(s) 210
TspDTI ATGAA 4 cut(s) 118, 173, 297, 1002
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 961
VneI GTGCAC 2 cut(s) 509, 701
VspI ATTAAT 1 cut(s) 429
XapI RAATTY 2 cut(s) 163, 568
XceI RCATGY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 5, 552, 994
XspI CTAG 3 cut(s) 225, 237, 1043
Zsp2I ATGCAT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.