Rroxscaffold_4G00309600

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000234
Physical Location & Seq
Forward (+)
31375554 .. 31376893
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309600.1

Sequence Viewer

Length: 1056 bp
ATGGACTCGAACTCATTGATTGAGAGGTGTCAATGGCTCCTTAATACGGGAACAAATGAGACTCTGATTAGTACCCCAGATTTCATTAATGCCCATCTAGAGAGGACTTCCATGAAAAGTACCCATGATCGTTTGCTTATCCAAACTAGGGAGCGTAGGATGGATAGAAAGTGCTTGTCACTCTTCTGTGCTGATACATTTGCCAAGTTTTCGGAGATAGAGCTTCCTATGATCAAAATTGAATCACATTTCCGCGTTGTGGTGGGTTCCTACAATGGAGTGGTTTGCTTATATGATAGTGATTTTGAAACTTATCTATGGAATCCATCAATCAGAAAATTCAAGAGACTTCCCCAGGTCCTCAATGATAGAAGGGGGCAATTAGCTCACTTTGCTATCTCGTTCGGGTTCCATCCTGAAGGTGATGACTATAAAGTTGTGAGAATTTTGACATTTGAGCGTCGTTATATGATTGAGGTTGAGGTTTACAGCCATAGGTTGAAGTCTTGGAGAAGAATTAATGCAGTTCCCCCTAATTCACATGAACTGTATATTCAGGGAGAAGGCACATGTGTGAATGGAGTTGTGTACTGGGTCATAAAAGAGTCTTTCCCATCCTGCAACTCCATCCTTTCTTTTGATCTAGGCAATGAGAATTTTCAAACAGTGAGGCTTTCTGATAGTCTGCTCAGGGCGATTGGTGATCCACGGCGTTTTCCAGTTAGTATTCGAGTGTTTGAGAAATCCCTTTCTTTGTTTCATGAAAGACGAGAGGAGAACAGTCGGTACTGTGACATATGGGTTCTGGAAATGGGTAGTTGGAAATTGTTTCACACAATTCCTCTCCCAGCTCGTGGATGTATAGCCTGGCCAGTGGGCTTTGCATCAAATGGTCAAGTTCATATTGTGATGTCAAGTAGAAAGTTTCGCAGCAAAACATTGGTTTTATATGATCTCAAATCACAACGAGTAAAAGATTTTAAAATCAAACTGCATGCGTATAGACCAGGCGGATACATCGATTATTTTAGGGAGAGTTTGGTGTTACTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

41.11

Weight (kDa)

9.11

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 83 - 330 6.5e-23 F-box associated beta propeller domain
FBA_1 PF07734 91 - 303 1.3e-18 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 854
AccII CGCG 1 cut(s) 255
AciI CCGC 2 cut(s) 253, 1011
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 3 cut(s) 338, 444, 655
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 4 cut(s) 73, 121, 590, 788
AfiI CCNNNNNNNGG 4 cut(s) 46, 148, 259, 854
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 5 cut(s) 242, 308, 343, 502, 662
AjnI CCWGG 3 cut(s) 354, 866, 1006
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 3 cut(s) 223, 386, 851
AluI AGCT 3 cut(s) 223, 386, 851
Alw26I GTCTC 2 cut(s) 53, 340
AlwI GGATC 1 cut(s) 698
AoxI GGCC 1 cut(s) 869
ApeKI GCWGC 1 cut(s) 930
ApoI RAATTY 3 cut(s) 338, 444, 655
AseI ATTAAT 2 cut(s) 87, 519
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 2 cut(s) 434, 713
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 871
BauI CACGAG 1 cut(s) 852
BbvI GCAGC 1 cut(s) 942
BccI CCATC 6 cut(s) 102, 154, 334, 420, 622, 635
BceAI ACGGC 1 cut(s) 725
BciT130I CCWGG 3 cut(s) 356, 868, 1008
BciVI GTATCC 1 cut(s) 1007
BclI TGATCA 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 53, 340
BfaI CTAG 3 cut(s) 98, 147, 644
BfuI GTATCC 1 cut(s) 1007
BisI GCNGC 1 cut(s) 931
BlsI GCNGC 1 cut(s) 932
Bme1390I CCNGG 3 cut(s) 356, 868, 1008
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 3 cut(s) 38, 268, 410
BmrFI CCNGG 3 cut(s) 356, 868, 1008
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 1 cut(s) 893
BmuI ACTGGG 1 cut(s) 601
Bpu10I CCTNAGC 1 cut(s) 689
Bsa29I ATCGAT 1 cut(s) 1020
BsaJI CCNNGG 2 cut(s) 354, 707
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 148, 259, 854
Bse1I ACTGG 3 cut(s) 596, 719, 872
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 3 cut(s) 356, 868, 1008
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 2 cut(s) 354, 707
BseGI GGATG 5 cut(s) 165, 412, 614, 627, 863
BseLI CCNNNNNNNGG 4 cut(s) 46, 148, 259, 854
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 1 cut(s) 703
BseNI ACTGG 3 cut(s) 596, 719, 872
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 1 cut(s) 942
BseYI CCCAGC 1 cut(s) 847
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 871
BshVI ATCGAT 1 cut(s) 1020
BslI CCNNNNNNNGG 4 cut(s) 46, 148, 259, 854
BsmAI GTCTC 2 cut(s) 53, 340
BsnI GGCC 1 cut(s) 871
Bsp143I GATC 5 cut(s) 127, 231, 640, 703, 952
BspACI CCGC 2 cut(s) 253, 1011
BspANI GGCC 1 cut(s) 871
BspCNI CTCAG 1 cut(s) 702
BspDI ATCGAT 1 cut(s) 1020
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 38, 268, 410
BspPI GGATC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 655
BsrI ACTGG 3 cut(s) 596, 719, 872
BssECI CCNNGG 2 cut(s) 354, 707
BssMI GATC 5 cut(s) 127, 231, 640, 703, 952
BssSI CACGAG 1 cut(s) 852
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 3 cut(s) 356, 868, 1008
Bst4CI ACNGT 4 cut(s) 549, 667, 782, 791
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 996
BstDEI CTNAG 1 cut(s) 689
BstDSI CCRYGG 1 cut(s) 707
BstF5I GGATG 5 cut(s) 165, 412, 614, 627, 863
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 5 cut(s) 130, 234, 643, 706, 955
BstMAI GTCTC 2 cut(s) 53, 340
BstMBI GATC 5 cut(s) 127, 231, 640, 703, 952
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 3 cut(s) 356, 868, 1008
BstNSI RCATGY 2 cut(s) 573, 998
BstSCI CCNGG 3 cut(s) 354, 866, 1006
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 942
Bsu15I ATCGAT 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 1 cut(s) 871
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 5 cut(s) 165, 412, 614, 627, 863
BtsIMutI CAGTG 2 cut(s) 672, 879
Cac8I GCNNGC 1 cut(s) 996
CciI TCATGA 1 cut(s) 760
Cfr13I GGNCC 1 cut(s) 358
ClaI ATCGAT 1 cut(s) 1020
CseI GACGC 1 cut(s) 449
Csp6I GTAC 4 cut(s) 72, 120, 589, 787
CviAII CATG 6 cut(s) 112, 125, 542, 570, 761, 995
CviJI RGCY 9 cut(s) 37, 223, 386, 492, 673, 851, 866, 871, 879
CviKI_1 RGCY 9 cut(s) 37, 223, 386, 492, 673, 851, 866, 871, 879
CviQI GTAC 4 cut(s) 72, 120, 589, 787
DdeI CTNAG 1 cut(s) 689
DpnI GATC 5 cut(s) 129, 233, 642, 705, 954
DpnII GATC 5 cut(s) 127, 231, 640, 703, 952
DraI TTTAAA 1 cut(s) 982
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 1026
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 3 cut(s) 354, 866, 1006
FaeI CATG 6 cut(s) 115, 128, 545, 573, 764, 998
FatI CATG 6 cut(s) 111, 124, 541, 569, 760, 994
FauNDI CATATG 1 cut(s) 797
FbaI TGATCA 1 cut(s) 231
Fnu4HI GCNGC 1 cut(s) 931
FokI GGATG 5 cut(s) 172, 399, 601, 614, 870
Fsp4HI GCNGC 1 cut(s) 931
FspBI CTAG 3 cut(s) 98, 147, 644
GluI GCNGC 1 cut(s) 931
GsaI CCCAGC 1 cut(s) 851
HaeIII GGCC 1 cut(s) 871
HgaI GACGC 1 cut(s) 449
Hin1II CATG 6 cut(s) 115, 128, 545, 573, 764, 998
HinfI GANTC 5 cut(s) 5, 61, 242, 322, 605
HphI GGTGA 2 cut(s) 434, 713
Hpy166II GTNNAC 2 cut(s) 487, 589
Hpy188I TCNGA 4 cut(s) 66, 214, 335, 679
Hpy188III TCNNGA 5 cut(s) 98, 343, 416, 761, 806
Hpy8I GTNNAC 2 cut(s) 487, 589
Hpy99I CGWCG 1 cut(s) 465
HpyAV CCTTC 3 cut(s) 366, 413, 557
HpyCH4III ACNGT 4 cut(s) 549, 667, 782, 791
HpyCH4V TGCA 4 cut(s) 524, 621, 884, 994
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 1 cut(s) 689
Hsp92II CATG 6 cut(s) 115, 128, 545, 573, 764, 998
Ksp22I TGATCA 1 cut(s) 231
Kzo9I GATC 5 cut(s) 127, 231, 640, 703, 952
LmnI GCTCC 2 cut(s) 42, 151
Lsp1109I GCAGC 1 cut(s) 942
LweI GCATC 1 cut(s) 893
MaeI CTAG 3 cut(s) 98, 147, 644
MaeIII GTNAC 3 cut(s) 177, 791, 1044
MalI GATC 5 cut(s) 129, 233, 642, 705, 954
MboI GATC 5 cut(s) 127, 231, 640, 703, 952
MboII GAAGA 2 cut(s) 175, 525
MlsI TGGCCA 1 cut(s) 871
MluCI AATT 9 cut(s) 237, 338, 380, 444, 516, 535, 655, 824, 837
MluNI TGGCCA 1 cut(s) 871
MlyI GAGTC 2 cut(s) 55, 614
MmeI TCCRAC 1 cut(s) 800
MnlI CCTC 8 cut(s) 18, 96, 371, 469, 475, 663, 766, 852
Mox20I TGGCCA 1 cut(s) 871
MscI TGGCCA 1 cut(s) 871
MseI TTAA 4 cut(s) 42, 87, 519, 981
MslI CAYNNNNRTG 1 cut(s) 572
Msp20I TGGCCA 1 cut(s) 871
MspR9I CCNGG 3 cut(s) 356, 868, 1008
MvaI CCWGG 3 cut(s) 356, 868, 1008
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeI CATATG 1 cut(s) 797
NdeII GATC 5 cut(s) 127, 231, 640, 703, 952
NlaIII CATG 6 cut(s) 115, 128, 545, 573, 764, 998
NlaIV GGNNCC 3 cut(s) 38, 268, 410
NmuCI GTSAC 2 cut(s) 177, 791
NspI RCATGY 2 cut(s) 573, 998
OliI CACNNNNGTG 1 cut(s) 572
PaeI GCATGC 1 cut(s) 998
PagI TCATGA 1 cut(s) 760
PciI ACATGT 1 cut(s) 569
PfeI GAWTC 2 cut(s) 242, 322
PflMI CCANNNNNTGG 1 cut(s) 854
PkrI GCNGC 1 cut(s) 932
PleI GAGTC 2 cut(s) 55, 613
PpsI GAGTC 2 cut(s) 55, 613
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 569
PshBI ATTAAT 2 cut(s) 87, 519
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 3 cut(s) 354, 866, 1006
PspFI CCCAGC 1 cut(s) 847
PspGI CCWGG 3 cut(s) 354, 866, 1006
PspN4I GGNNCC 3 cut(s) 38, 268, 410
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PsrI GAACNNNNNNTAC 2 cut(s) 770, 802
RsaI GTAC 4 cut(s) 73, 121, 590, 788
RsaNI GTAC 4 cut(s) 72, 120, 589, 787
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 4 cut(s) 42, 87, 519, 981
SatI GCNGC 1 cut(s) 931
Sau3AI GATC 5 cut(s) 127, 231, 640, 703, 952
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 2 cut(s) 55, 614
ScrFI CCNGG 3 cut(s) 356, 868, 1008
SetI ASST 9 cut(s) 29, 225, 360, 388, 424, 480, 486, 500, 853
SfaNI GCATC 1 cut(s) 893
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 572
SphI GCATGC 1 cut(s) 998
Sse9I AATT 9 cut(s) 237, 338, 380, 444, 516, 535, 655, 824, 837
SsiI CCGC 2 cut(s) 253, 1011
SspMI CTAG 3 cut(s) 98, 147, 644
StyD4I CCNGG 3 cut(s) 354, 866, 1006
TaaI ACNGT 4 cut(s) 549, 667, 782, 791
TaqI TCGA 4 cut(s) 8, 730, 1020, 1050
TasI AATT 9 cut(s) 237, 338, 380, 444, 516, 535, 655, 824, 837
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 2 cut(s) 242, 322
Tru1I TTAA 4 cut(s) 42, 87, 519, 981
Tru9I TTAA 4 cut(s) 42, 87, 519, 981
TscAI CASTG 2 cut(s) 672, 879
TseFI GTSAC 2 cut(s) 177, 791
TseI GCWGC 1 cut(s) 930
Tsp45I GTSAC 2 cut(s) 177, 791
TspDTI ATGAA 6 cut(s) 73, 128, 558, 749, 777, 890
TspRI CASTG 2 cut(s) 672, 879
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 2 cut(s) 87, 519
XapI RAATTY 3 cut(s) 338, 444, 655
XbaI TCTAGA 1 cut(s) 97
XceI RCATGY 2 cut(s) 573, 998
XspI CTAG 3 cut(s) 98, 147, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.