RLG00000020056

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000234
Physical Location & Seq
Reverse (-)
60630395 .. 60632612
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020056

Sequence Viewer

Length: 1053 bp
ATGACGTCCACAATTTGTAAGAAAGAAATCTTGATGGACATCCTAGTAAGACTACCTGCAAAAACCCTAATGCGGTTCTTATGTGCATGCAAAGCATGGAGTGATTTGATTAGCAGCCCAGATTTTGTCTCTACAAATCTTAACACAAATATCACAAAACGCAGAAATATGCATCTACTCTGCCTCCACCACCCGGATTTTAAACGGGTGGTCGATTTGGAGGATCCATATGTTAAACAAGCTCTTCAATGGTCTTTCTTTCACTATGAAACATTTGAGCAGGGCTTGGGGTTAAGCCATCCCTCAGGGAGCACAGAACATTATGGGATGTATGGTTCAAGCAATGGATTAGTTTGCATTTCAAATCCGGACGAAGTTTTGAATAGCAGGAGTCCTATAATCATATGGAACCCGTCCATTAAGAAATTTAAGACGCTTCCATCGACCACAAACAATAAATTTCGCTATATGTCCCTCCAATTTGGCTTCCATCCCTGGGTTAATGACTACAAGGTAGTAAGAACGATGCGGATCAACAAGGATGCCTTCGCAGTAGAAGTTTATTCTCTTAGCACCAACTCTTGGAAGATGATCGAAGAAATTCCTCCTTGGTTAAAATGCAATTTTAAATATCTTGAAGGTACGTTCATAAATGGAGTAGCCTACCGAATTCTTGAGAAAGGTTTTATGTATAGCCTCATGTCCTTCGATTCGGGCAGTGAAGAATTTGAAGAATTCATACTACCAGAGTCCGTCATCAGTTCACATAATTTACATATCTACCCTTTCGAGGAAAAGCCTTGCTTGATTTTTCACTGGTATATTTCTGATGAGGAGGGCTTTGATAGAGGCTTTCTTTTGCAGCGGCGGCGATTTTACAACAAGTGTCTGGTCTTGGTGTTGCAGCGCTGGGGGTGGTGCGTCCTGCCACTTGATCGTGAGCGACTAGAGAAGATGGGTTGGAAGTACTGGCTGACGGAGACAGTGGCAGTGACGAGTTGGAAGGCGGTGCATGGTTATCCTCCTGATAGCCATGCTTGTGTATGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

351

Amino Acids

41.09

Weight (kDa)

7.98

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 106 - 267 1.4e-19 F-box associated beta propeller domain
FBA_1 PF07734 114 - 249 5.8e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 5 cut(s) 73, 529, 865, 868, 1007
AclWI GGATC 3 cut(s) 218, 231, 539
AcsI RAATTY 6 cut(s) 425, 458, 600, 669, 725, 734
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 643, 968
AfeI AGCGCT 1 cut(s) 908
AfiI CCNNNNNNNGG 5 cut(s) 72, 193, 496, 582, 790
AgsI TTSAA 6 cut(s) 248, 339, 363, 382, 638, 731
AjnI CCWGG 1 cut(s) 494
AloI GAACNNNNNNTCC 2 cut(s) 319, 351
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 2 cut(s) 133, 974
AlwI GGATC 3 cut(s) 218, 231, 539
Aor13HI TCCGGA 1 cut(s) 367
Aor51HI AGCGCT 1 cut(s) 908
AoxI GGCC 1 cut(s) 1047
ApeKI GCWGC 3 cut(s) 114, 862, 904
ApoI RAATTY 6 cut(s) 425, 458, 600, 669, 725, 734
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 1 cut(s) 1047
AsuC2I CCSGG 1 cut(s) 194
AxyI CCTNAGG 1 cut(s) 304
BamHI GGATCC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 126, 874, 916
BccI CCATC 5 cut(s) 28, 306, 448, 498, 949
BciT130I CCWGG 1 cut(s) 496
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 133, 974
BfaI CTAG 2 cut(s) 44, 947
BfoI RGCGCY 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 64
BisI GCNGC 5 cut(s) 115, 863, 866, 869, 905
BlsI GCNGC 5 cut(s) 116, 864, 867, 870, 906
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 2 cut(s) 194, 496
BmgT120I GGNCC 1 cut(s) 1047
BmiI GGNNCC 2 cut(s) 225, 410
BmrFI CCNGG 2 cut(s) 194, 496
BmsI GCATC 3 cut(s) 181, 516, 532
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 1 cut(s) 194
BsaBI GATNNNNATC 2 cut(s) 38, 530
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 494, 495, 608
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 193, 496, 582, 790
Bse1I ACTGG 2 cut(s) 821, 974
Bse21I CCTNAGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 349
Bse8I GATNNNNATC 2 cut(s) 38, 530
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 3 cut(s) 494, 495, 608
BseGI GGATG 5 cut(s) 39, 298, 333, 490, 547
BseJI GATNNNNATC 2 cut(s) 38, 530
BseLI CCNNNNNNNGG 5 cut(s) 72, 193, 496, 582, 790
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 2 cut(s) 821, 974
BseRI GAGGAG 1 cut(s) 848
BseXI GCAGC 3 cut(s) 126, 874, 916
BseYI CCCAGC 1 cut(s) 909
BshFI GGCC 1 cut(s) 1049
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 2 cut(s) 194, 368
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 5 cut(s) 72, 193, 496, 582, 790
BsmAI GTCTC 2 cut(s) 133, 974
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 1 cut(s) 1049
Bsp1286I GDGCHC 1 cut(s) 314
Bsp13I TCCGGA 1 cut(s) 367
Bsp143I GATC 4 cut(s) 223, 531, 591, 934
BspACI CCGC 5 cut(s) 73, 529, 865, 868, 1007
BspANI GGCC 1 cut(s) 1049
BspCNI CTCAG 1 cut(s) 317
BspEI TCCGGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 225, 410
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 3 cut(s) 218, 231, 539
BspQI GCTCTTC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 2 cut(s) 821, 974
BssECI CCNNGG 3 cut(s) 494, 495, 608
BssMI GATC 4 cut(s) 223, 531, 591, 934
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 985
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 2 cut(s) 304, 569
BstF5I GGATG 5 cut(s) 39, 298, 333, 490, 547
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 4 cut(s) 226, 534, 594, 937
BstMAI GTCTC 2 cut(s) 133, 974
BstMBI GATC 4 cut(s) 223, 531, 591, 934
BstMWI GCNNNNNNNGC 2 cut(s) 92, 868
BstNI CCWGG 1 cut(s) 496
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 192, 494
BstV1I GCAGC 3 cut(s) 126, 874, 916
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
Bsu36I CCTNAGG 1 cut(s) 304
BsuRI GGCC 1 cut(s) 1049
BtsCI GGATG 5 cut(s) 39, 298, 333, 490, 547
BtsI GCAGTG 2 cut(s) 724, 996
BtsIMutI CAGTG 4 cut(s) 724, 814, 990, 996
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 1 cut(s) 88
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 1 cut(s) 1047
CseI GACGC 2 cut(s) 442, 910
Csp6I GTAC 2 cut(s) 642, 967
CviAII CATG 5 cut(s) 87, 96, 700, 1013, 1034
CviQI GTAC 2 cut(s) 642, 967
DdeI CTNAG 2 cut(s) 304, 569
DpnI GATC 4 cut(s) 225, 533, 593, 936
DpnII GATC 4 cut(s) 223, 531, 591, 934
DraI TTTAAA 2 cut(s) 202, 628
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 608
Eco47III AGCGCT 1 cut(s) 908
Eco81I CCTNAGG 1 cut(s) 304
EcoRI GAATTC 2 cut(s) 669, 734
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 5 cut(s) 90, 99, 703, 1016, 1037
FalI AAGNNNNNCTT 4 cut(s) 530, 562, 788, 820
FaqI GGGAC 1 cut(s) 457
FatI CATG 5 cut(s) 86, 95, 699, 1012, 1033
FauNDI CATATG 2 cut(s) 229, 404
Fnu4HI GCNGC 5 cut(s) 115, 863, 866, 869, 905
FokI GGATG 5 cut(s) 26, 285, 340, 477, 554
Fsp4HI GCNGC 5 cut(s) 115, 863, 866, 869, 905
FspBI CTAG 2 cut(s) 44, 947
GlaI GCGC 1 cut(s) 908
GluI GCNGC 5 cut(s) 115, 863, 866, 869, 905
GsaI CCCAGC 1 cut(s) 913
HaeII RGCGCY 1 cut(s) 910
HaeIII GGCC 1 cut(s) 1049
HapII CCGG 2 cut(s) 194, 368
HgaI GACGC 2 cut(s) 442, 910
HhaI GCGC 1 cut(s) 909
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 90, 99, 703, 1016, 1037
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HinfI GANTC 3 cut(s) 391, 710, 749
HpaII CCGG 2 cut(s) 194, 368
Hpy166II GTNNAC 2 cut(s) 9, 764
Hpy188I TCNGA 1 cut(s) 829
Hpy188III TCNNGA 6 cut(s) 31, 368, 635, 674, 938, 1025
Hpy8I GTNNAC 2 cut(s) 9, 764
HpyAV CCTTC 4 cut(s) 556, 632, 715, 997
HpyCH4III ACNGT 1 cut(s) 985
HpyCH4IV ACGT 2 cut(s) 5, 644
HpyCH4V TGCA 9 cut(s) 59, 86, 90, 172, 357, 621, 862, 904, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 868
HpyF3I CTNAG 2 cut(s) 304, 569
HpySE526I ACGT 2 cut(s) 5, 644
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 90, 99, 703, 1016, 1037
HspAI GCGC 1 cut(s) 907
Kpn2I TCCGGA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 223, 531, 591, 934
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 309
Lsp1109I GCAGC 3 cut(s) 126, 874, 916
LweI GCATC 3 cut(s) 181, 516, 532
MaeI CTAG 2 cut(s) 44, 947
MaeII ACGT 2 cut(s) 5, 644
MaeIII GTNAC 1 cut(s) 991
MalI GATC 4 cut(s) 225, 533, 593, 936
MboI GATC 4 cut(s) 223, 531, 591, 934
MboII GAAGA 6 cut(s) 236, 598, 608, 734, 743, 964
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 314
MlyI GAGTC 2 cut(s) 400, 758
MmeI TCCRAC 2 cut(s) 941, 980
Mph1103I ATGCAT 1 cut(s) 174
MroI TCCGGA 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 9 cut(s) 141, 201, 234, 293, 420, 429, 501, 614, 627
MslI CAYNNNNRTG 1 cut(s) 1038
MspA1I CMGCKG 1 cut(s) 865
MspI CCGG 2 cut(s) 194, 368
MspR9I CCNGG 2 cut(s) 194, 496
MvaI CCWGG 1 cut(s) 496
MwoI GCNNNNNNNGC 2 cut(s) 92, 868
NciI CCSGG 1 cut(s) 194
NdeI CATATG 2 cut(s) 229, 404
NdeII GATC 4 cut(s) 223, 531, 591, 934
NlaIII CATG 5 cut(s) 90, 99, 703, 1016, 1037
NlaIV GGNNCC 2 cut(s) 225, 410
NmuCI GTSAC 1 cut(s) 991
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 1 cut(s) 90
PaeI GCATGC 1 cut(s) 90
PasI CCCWGGG 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 710
PflMI CCANNNNNTGG 1 cut(s) 582
PkrI GCNGC 5 cut(s) 116, 864, 867, 870, 906
PleI GAGTC 2 cut(s) 399, 757
PpsI GAGTC 2 cut(s) 399, 757
Psp6I CCWGG 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 909
PspGI CCWGG 1 cut(s) 494
PspN4I GGNNCC 2 cut(s) 225, 410
PspPI GGNCC 1 cut(s) 1047
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 2 cut(s) 643, 968
RsaNI GTAC 2 cut(s) 642, 967
RseI CAYNNNNRTG 1 cut(s) 1038
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 9 cut(s) 141, 201, 234, 293, 420, 429, 501, 614, 627
SatI GCNGC 5 cut(s) 115, 863, 866, 869, 905
Sau3AI GATC 4 cut(s) 223, 531, 591, 934
Sau96I GGNCC 1 cut(s) 1047
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 2 cut(s) 400, 758
ScrFI CCNGG 2 cut(s) 194, 496
SduI GDGCHC 1 cut(s) 314
SetI ASST 7 cut(s) 8, 58, 244, 516, 643, 647, 685
SfaNI GCATC 3 cut(s) 181, 516, 532
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SphI GCATGC 1 cut(s) 90
SsiI CCGC 5 cut(s) 73, 529, 865, 868, 1007
SspMI CTAG 2 cut(s) 44, 947
StyD4I CCNGG 2 cut(s) 192, 494
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 1 cut(s) 985
TaiI ACGT 2 cut(s) 8, 647
TaqI TCGA 5 cut(s) 213, 443, 594, 708, 789
TatI WGTACW 1 cut(s) 966
TauI GCSGC 2 cut(s) 868, 871
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 9 cut(s) 141, 201, 234, 293, 420, 429, 501, 614, 627
Tru9I TTAA 9 cut(s) 141, 201, 234, 293, 420, 429, 501, 614, 627
TscAI CASTG 4 cut(s) 724, 821, 990, 996
TseFI GTSAC 1 cut(s) 991
TseI GCWGC 3 cut(s) 114, 862, 904
Tsp45I GTSAC 1 cut(s) 991
TspDTI ATGAA 3 cut(s) 282, 637, 727
TspGWI ACGGA 2 cut(s) 742, 992
TspRI CASTG 4 cut(s) 724, 821, 990, 996
Van91I CCANNNNNTGG 1 cut(s) 582
XapI RAATTY 6 cut(s) 425, 458, 600, 669, 725, 734
XceI RCATGY 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 2 cut(s) 44, 947
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 968
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.