Rorug05G0292800

Glucomannan 4-beta-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000131
Physical Location & Seq
Forward (+)
33771985 .. 33776645
4661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0292800.1

Sequence Viewer

Length: 1752 bp
ATGGTCACGGATGCTAAAAACCCTCTTGGAGAAACCACCTGCGGTTCTTTGCTGCAGAAACTGCAGGAAATTTGGGATGAAGTTGGTGAGAGTGATGAGCAACGAGACAGAATGCTTCTGCAATTAGAGCAGGAGTGTCTGGATGTATACAAGAGAAAGGTTGAGCTGGCAGCAAAGTCAAGGGCACAGCTTCTTCAGGCATTGTCCGATGGCAAACTTGAGCTTTCCACTCTTCAATCAGCACTTGGAGAAAAAAGTTCTGTTGGAGTTCCAGAGAAGACTTCTGGAACAATCAAGGAACAGCTTGCGGCTATAGCACCAGCACTGGAACAGTTGTGGAAACAGAAAGAAGAGAGAGTAACCGAATTTTCTGATGTACAATCACAGATACAAAAGATTTGTGGAGAAATTGCTGGGAACCTTTCTCTCAGTGAGACTCCTGCTGTTGATGAGTCTGATCTGTCCCTGAAAAAGTTAGATGAATATCAATCCCAACTTCAAGATCTTCAAAAGGAAAAGAGTGAAAGGCTGCACAAGGTGCTGGAATTTGTGAGCACAGTGCATGATCTTTGTGCTGTACTTGGAATAGACTTCTACAGTACTGTTACAGAGGTTCATCCTAGCTTGAATGACTCAACTGGTGTGCAATCCAAAAGCATTAGCAATGAAACTCTATCCAGGCTGGATAAGACAGTCTTAGCACTAGAAGAAGATAAGAAGCAGAGGCTGCATAAGCTTCAAGAGTCGGCAGCACAGCTAATTGATCTCTGGAATCTGATGGATACCCCTGAAGAGGAAAGGAAACTGTTTGACCATGTTACCTGTAACATATCAGCTTCAGTTGATGAAGTGACAGTTCCTGGGGCCCTTGCGCCAGATCTAATTGAGCAGACTGAGATCGAAGTTGAGAGGCTTGATCAGTTGAAATCAAGCAGGATGAAGGAAATTGCATTTAAAAAGCAAGGGGAGCTTGAGGAGATTTTTGCTCGAGCGCACATAGAAATAGACACTGAAGCTGCCAGAGAAAAGATTATGGAACTCATTGATTCTGGGAGTGTTGAGCCTACTGAGTTACTGGCTGACATGGATGATCAGATAGCAAAAGCGAAGGATGAAGCTCTCAGCAGAAAGGACATATTGGACAAAGTTGAGAAATGGATGTCAGCCTGTGAAGAGGAGAGTTGGCTTGAGGACTACAATCGTGATGAAAACAGGTACAATGCAAGTAGAGGTGCACACCTAAATCTAAAGCGTGCAGAAAAAGCACGTATTCTGGTTAACAAAATTCCAGCTTTGGTTGACACACTGGTAGCCAAAACTCGTGTGTGGGAGGAAGAACGTGGCATATCATTTACTTATGATGGTGTTCCTCTTCTCGCTATGCTAGATGAATATGCCATGCTCAGGCAAGAAAGAGAAGAAGAGAAGCGGAGATTGAGGGATCAGAAGAAGTTCCAGGAGCTACAACACACAGAACAAGAAAGTGTATTTGGTTCAAAGCCTAGCCCTAGCCCTGCTCGACCAGTTGGCACAAAGAAGGTAGTAGGTCCTCGTGCAAATGGTGGTCCCAATGGGACTCCCAGACGACTTTCACTTAATGCTAATCAAAATGGAAGGTCGGCAGTGAAAGATGCAAAGAGGGATCCTAGACCTGTTGCTCCTATGAACTATGTTGAAGTATCGAAAGAGGATGCTGCATCCCACATTTCGGGAACTGGTTCTGGCAGTGACCCAATTCCGGCTTCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3 Glycos_transf_2

Protein Analysis

583

Amino Acids

65.26

Weight (kDa)

4.97

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 16 - 504 1.6e-85 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31980 AT5G11290 AT5G11290
fragaria_vesca FvH4_2g29230 FvH4_2g29230 FvH4_2g29310 FvH4_2g29351 FvH4_2g29352 FvH4_2g29352 FvH4_2g29362 FvH4_2g29370 FvH4_2g29461 FvH4_2g29461 FvH4_2g29461 FvH4_2g29461 FvH4_2g29470 FvH4_2g29490 FvH4_2g29490 FvH4_2g29500 FvH4_2g29500 FvH4_2g29520 FvH4_2g33600 FvH4_3g29960 FvH4_5g20260 FvH4_7g25070
malus_domestica MD00G1144600.v1.1 MD07G1131600.v1.1 MD08G1146300.v1.1 MD08G1146400.v1.1 MD08G1146500.v1.1 MD08G1146600.v1.1 MD15G1035400.v1.1 MD15G1100600.v1.1 MD15G1100700.v1.1 MD15G1101400.v1.1 MD15G1101800.v1.1 MD15G1118600.v1.1 MD15G1119800.v1.1 MD15G1121700.v1.1 MD15G1121800.v1.1 MD15G1122100.v1.1 MD15G1122400.v1.1 MD15G1122700.v1.1 MD15G1123000.v1.1
prunus_persica Prupe.1G474700_v2.0.a1 Prupe.1G474800_v2.0.a1 Prupe.1G475000_v2.0.a1 Prupe.1G475600_v2.0.a1 Prupe.1G476100_v2.0.a1 Prupe.1G476400_v2.0.a1 Prupe.1G476600_v2.0.a1 Prupe.1G476900_v2.0.a1 Prupe.1G477100_v2.0.a1 Prupe.1G477300_v2.0.a1 Prupe.1G477500_v2.0.a1 Prupe.1G477600_v2.0.a1 Prupe.1G477600_v2.0.a1 Prupe.1G477700_v2.0.a1 Prupe.1G477900_v2.0.a1 Prupe.1G478000_v2.0.a1 Prupe.2G074000_v2.0.a1 Prupe.7G095900_v2.0.a1 Prupe.7G095900_v2.0.a1 Prupe.7G095900_v2.0.a1 Prupe.7G096100_v2.0.a1 Prupe.7G096100_v2.0.a1 Prupe.7G096200_v2.0.a1 Prupe.7G096400_v2.0.a1 Prupe.8G015000_v2.0.a1
pyrus_communis pycom08g12340 pycom11g04600 pycom15g09280 pycom15g10770 pycom15g10790 pycom15g11000 pycom15g11040 pycom854g00030 pycom854g00050
rosa_chinensis RchiOBHm_Chr1g0321791 RchiOBHm_Chr3g0452021 RchiOBHm_Chr3g0452031 RchiOBHm_Chr3g0475821 RchiOBHm_Chr4g0410021 RchiOBHm_Chr4g0412191 RchiOBHm_Chr4g0415271 RchiOBHm_Chr6g0244001 RchiOBHm_Chr6g0252301 RchiOBHm_Chr6g0252341 RchiOBHm_Chr6g0267401 RchiOBHm_Chr6g0297251 RchiOBHm_Chr6g0298131 RchiOBHm_Chr6g0298141 RchiOBHm_Chr6g0298151 RchiOBHm_Chr6g0298161 RchiOBHm_Chr6g0298261 RchiOBHm_Chr6g0298271 RchiOBHm_Chr6g0298281 RchiOBHm_Chr6g0298291 RchiOBHm_Chr6g0298311 RchiOBHm_Chr6g0298321 RchiOBHm_Chr6g0298331 RchiOBHm_Chr6g0298341 RchiOBHm_Chr6g0298381 RchiOBHm_Chr6g0302351 RchiOBHm_Chr7g0203021 RchiOBHm_Chr7g0225421
rosa_laevigata RLG00000023811 RLG00000030588
rosa_multiflora Rmu_co8159872.1_g000001 Rmu_co8452635.1_g000001 Rmu_co8477209.1_g000001 Rmu_sc0000365.1_g000079 Rmu_sc0000461.1_g000042 Rmu_sc0000539.1_g000085 Rmu_sc0001358.1_g000006 Rmu_sc0001358.1_g000007 Rmu_sc0001673.1_g000024 Rmu_sc0002691.1_g000007 Rmu_sc0002724.1_g000011 Rmu_sc0003410.1_g000027 Rmu_sc0003420.1_g000007 Rmu_sc0003420.1_g000008 Rmu_sc0003420.1_g000009 Rmu_sc0003420.1_g000010 Rmu_sc0003551.1_g000014 Rmu_sc0004535.1_g000001 Rmu_sc0006252.1_g000006 Rmu_sc0006543.1_g000004 Rmu_sc0006711.1_g000015 Rmu_sc0007115.1_g000004 Rmu_sc0007115.1_g000011 Rmu_sc0007115.1_g000014 Rmu_sc0009317.1_g000003 Rmu_sc0009318.1_g000002 Rmu_sc0009318.1_g000003 Rmu_sc0010099.1_g000008 Rmu_sc0012041.1_g000004 Rmu_sc0013249.1_g000008 Rmu_sc0035583.1_g000001 Rmu_ssc0000004.1_g000024
rosa_roxburghii Rroxscaffold_1G00023390 Rroxscaffold_1G00024930 Rroxscaffold_3G00234420 Rroxscaffold_3G00234430 Rroxscaffold_5G00354530 Rroxscaffold_6G00405820 Rroxscaffold_6G00429190 Rroxscaffold_7G00165840 Rroxscaffold_7G00169700 Rroxscaffold_7G00169730 Rroxscaffold_7G00169740 Rroxscaffold_7G00169770 Rroxscaffold_7G00169780 Rroxscaffold_7G00169790 Rroxscaffold_7G00169800 Rroxscaffold_7G00170710 Rroxscaffold_7G00170720 Rroxscaffold_7G00185780 Rroxscaffold_7G00185800 Rroxscaffold_7G00201380 Rroxscaffold_7G00216170
rosa_rugosa Rorug03G0151700 Rorug03G0151700 Rorug05G0292700 Rorug05G0292800
rosa_samantha Rh1AG047000 Rh2BG283800 Rh2BG283900 Rh3BG041400 Rh3BG231700 Rh3DG227000 Rh4CG170100 Rh4CG170200 Rh4CG204700 Rh6AG020700 Rh6AG021000 Rh6AG381400 Rh6AG381500 Rh6AG389300 Rh6AG390400 Rh6AG390500 Rh6AG390600 Rh6AG390700 Rh6AG391200 Rh6AG391400 Rh7CG233300
rosa_wichuraiana Rw0G004890 Rw2G021490 Rw3G003000 Rw3G018320 Rw3G018340 Rw4G012210 Rw4G013140 Rw4G016490 Rw6G001700 Rw6G001720 Rw6G001730 Rw6G033220 Rw6G033230 Rw6G033990 Rw6G034080 Rw6G034090 Rw6G034100 Rw6G034110 Rw6G034140 Rw6G034180 Rw6G034190 Rw6G034200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 147
AciI CCGC 3 cut(s) 42, 308, 1429
AclWI GGATC 3 cut(s) 1449, 1637, 1650
AcsI RAATTY 4 cut(s) 69, 365, 545, 1284
AcuI CTGAAG 4 cut(s) 179, 810, 822, 1032
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 4 cut(s) 378, 579, 601, 1217
AfiI CCNNNNNNNGG 4 cut(s) 793, 1404, 1708, 1738
AgsI TTSAA 8 cut(s) 236, 500, 509, 628, 740, 925, 1497, 1676
AjnI CCWGG 3 cut(s) 677, 859, 1455
AjuI GAANNNNNNNTTGG 3 cut(s) 1473, 1505, 1726
Alw21I GWGCWC 2 cut(s) 557, 1237
Alw26I GTCTC 2 cut(s) 99, 428
Alw44I GTGCAC 1 cut(s) 1233
AlwI GGATC 3 cut(s) 1449, 1637, 1650
AlwNI CAGNNNCTG 3 cut(s) 61, 727, 860
Ama87I CYCGRG 1 cut(s) 987
AoxI GGCC 1 cut(s) 864
ApaI GGGCCC 1 cut(s) 868
ApaLI GTGCAC 1 cut(s) 1233
ApeKI GCWGC 7 cut(s) 52, 170, 529, 727, 749, 1016, 1694
ApoI RAATTY 4 cut(s) 69, 365, 545, 1284
Asp700I GAANNNNTTC 2 cut(s) 1451, 1717
AspLEI GCGC 2 cut(s) 874, 994
AspS9I GGNCC 4 cut(s) 864, 865, 1547, 1565
AsuHPI GGTGA 2 cut(s) 98, 1737
AvaI CYCGRG 1 cut(s) 987
AvaII GGWCC 2 cut(s) 1547, 1565
BaeGI GKGCMC 3 cut(s) 187, 868, 1237
BamHI GGATCC 1 cut(s) 1642
BanII GRGCYC 1 cut(s) 868
BauI CACGAG 2 cut(s) 1320, 1551
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 557, 1237
BbvI GCAGC 7 cut(s) 39, 182, 516, 714, 761, 1003, 1681
BccI CCATC 3 cut(s) 203, 772, 1355
BciT130I CCWGG 3 cut(s) 679, 861, 1457
BciVI GTATCC 1 cut(s) 775
BclI TGATCA 2 cut(s) 916, 1090
BcoDI GTCTC 2 cut(s) 99, 428
BfaI CTAG 6 cut(s) 621, 704, 1385, 1503, 1509, 1647
BfmI CTRYAG 4 cut(s) 53, 62, 312, 595
BfuAI ACCTGC 1 cut(s) 47
BfuI GTATCC 1 cut(s) 775
BglII AGATCT 2 cut(s) 502, 877
BisI GCNGC 8 cut(s) 53, 171, 309, 530, 728, 750, 1017, 1695
BlsI GCNGC 8 cut(s) 54, 172, 310, 531, 729, 751, 1018, 1696
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 3 cut(s) 679, 861, 1457
Bme18I GGWCC 2 cut(s) 1547, 1565
BmeT110I CYCGRG 1 cut(s) 987
BmgT120I GGNCC 4 cut(s) 864, 865, 1547, 1565
BmiI GGNNCC 5 cut(s) 419, 865, 866, 1567, 1644
BmrFI CCNGG 3 cut(s) 679, 861, 1457
BmsI GCATC 3 cut(s) 1621, 1681, 1706
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 1403
BpuEI CTTGAG 3 cut(s) 239, 992, 1208
BsaAI YACGTR 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 860
Bsc4I CCNNNNNNNGG 4 cut(s) 793, 1404, 1708, 1738
Bse1I ACTGG 6 cut(s) 330, 643, 1080, 1311, 1523, 1720
Bse3DI GCAATG 1 cut(s) 670
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 3 cut(s) 679, 861, 1457
BseDI CCNNGG 1 cut(s) 860
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 793, 1404, 1708, 1738
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 5 cut(s) 442, 885, 1059, 1135, 1417
BseNI ACTGG 6 cut(s) 330, 643, 1080, 1311, 1523, 1720
BseRI GAGGAG 2 cut(s) 989, 1190
BseSI GKGCMC 3 cut(s) 187, 868, 1237
BseXI GCAGC 7 cut(s) 39, 182, 516, 714, 761, 1003, 1681
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 2 cut(s) 515, 1275
BshFI GGCC 1 cut(s) 866
BsiHKAI GWGCWC 2 cut(s) 557, 1237
BsiHKCI CYCGRG 1 cut(s) 987
BsiSI CCGG 1 cut(s) 1739
BslFI GGGAC 3 cut(s) 448, 1551, 1588
BslI CCNNNNNNNGG 4 cut(s) 793, 1404, 1708, 1738
BsmAI GTCTC 2 cut(s) 99, 428
BsmFI GGGAC 3 cut(s) 448, 1551, 1588
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 1 cut(s) 866
BsoBI CYCGRG 1 cut(s) 987
Bsp120I GGGCCC 1 cut(s) 864
Bsp1286I GDGCHC 4 cut(s) 187, 557, 868, 1237
Bsp1407I TGTACA 1 cut(s) 376
BspACI CCGC 3 cut(s) 42, 308, 1429
BspANI GGCC 1 cut(s) 866
BspCNI CTCAG 5 cut(s) 441, 886, 1060, 1134, 1416
BspLI GGNNCC 5 cut(s) 419, 865, 866, 1567, 1644
BspMAI CTGCAG 2 cut(s) 57, 66
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 1449, 1637, 1650
BsrDI GCAATG 1 cut(s) 670
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 6 cut(s) 330, 643, 1080, 1311, 1523, 1720
BssECI CCNNGG 1 cut(s) 860
BssNAI GTATAC 1 cut(s) 148
BssSI CACGAG 2 cut(s) 1320, 1551
Bst1107I GTATAC 1 cut(s) 148
Bst2BI CACGAG 2 cut(s) 1320, 1551
Bst2UI CCWGG 3 cut(s) 679, 861, 1457
Bst4CI ACNGT 7 cut(s) 333, 559, 599, 604, 694, 807, 856
Bst6I CTCTTC 6 cut(s) 237, 345, 786, 1167, 1377, 1416
BstAPI GCANNNNNTGC 3 cut(s) 61, 538, 727
BstAUI TGTACA 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 1268
BstC8I GCNNGC 3 cut(s) 168, 306, 1254
BstDEI CTNAG 6 cut(s) 428, 697, 894, 1068, 1121, 1403
BstHHI GCGC 2 cut(s) 874, 994
BstMAI GTCTC 2 cut(s) 99, 428
BstMWI GCNNNNNNNGC 8 cut(s) 61, 127, 314, 538, 727, 733, 967, 1262
BstNI CCWGG 3 cut(s) 679, 861, 1457
BstSCI CCNGG 3 cut(s) 677, 859, 1455
BstSFI CTRYAG 4 cut(s) 53, 62, 312, 595
BstSLI GKGCMC 3 cut(s) 187, 868, 1237
BstV1I GCAGC 7 cut(s) 39, 182, 516, 714, 761, 1003, 1681
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 3 cut(s) 502, 877, 1642
BstYI RGATCY 3 cut(s) 502, 877, 1642
BstZ17I GTATAC 1 cut(s) 148
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 1 cut(s) 866
BtsI GCAGTG 2 cut(s) 1629, 1732
BtsIMutI CAGTG 7 cut(s) 323, 436, 564, 1008, 1304, 1629, 1732
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 3 cut(s) 168, 306, 1254
CaiI CAGNNNCTG 3 cut(s) 61, 727, 860
CfoI GCGC 2 cut(s) 874, 994
Cfr13I GGNCC 4 cut(s) 864, 865, 1547, 1565
Csp6I GTAC 4 cut(s) 377, 578, 600, 1216
CviAII CATG 4 cut(s) 563, 815, 1084, 1399
CviQI GTAC 4 cut(s) 377, 578, 600, 1216
DdeI CTNAG 6 cut(s) 428, 697, 894, 1068, 1121, 1403
DraI TTTAAA 1 cut(s) 955
DraIII CACNNNGTG 1 cut(s) 538
Eam1104I CTCTTC 6 cut(s) 237, 345, 786, 1167, 1377, 1416
EarI CTCTTC 6 cut(s) 237, 345, 786, 1167, 1377, 1416
Eco24I GRGCYC 1 cut(s) 868
Eco47I GGWCC 2 cut(s) 1547, 1565
Eco57I CTGAAG 4 cut(s) 179, 810, 822, 1032
Eco88I CYCGRG 1 cut(s) 987
EcoO109I RGGNCCY 3 cut(s) 864, 865, 1547
EcoRII CCWGG 3 cut(s) 677, 859, 1455
EcoT38I GRGCYC 1 cut(s) 868
FaeI CATG 4 cut(s) 566, 818, 1087, 1402
FalI AAGNNNNNCTT 4 cut(s) 680, 712, 954, 986
FaqI GGGAC 3 cut(s) 448, 1551, 1588
FatI CATG 4 cut(s) 562, 814, 1083, 1398
FbaI TGATCA 2 cut(s) 916, 1090
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 8 cut(s) 53, 171, 309, 530, 728, 750, 1017, 1695
FriOI GRGCYC 1 cut(s) 868
Fsp4HI GCNGC 8 cut(s) 53, 171, 309, 530, 728, 750, 1017, 1695
FspBI CTAG 6 cut(s) 621, 704, 1385, 1503, 1509, 1647
GlaI GCGC 2 cut(s) 873, 993
GluI GCNGC 8 cut(s) 53, 171, 309, 530, 728, 750, 1017, 1695
GsaI CCCAGC 1 cut(s) 417
HaeIII GGCC 1 cut(s) 866
HapII CCGG 1 cut(s) 1739
HhaI GCGC 2 cut(s) 874, 994
Hin1II CATG 4 cut(s) 566, 818, 1087, 1402
Hin6I GCGC 2 cut(s) 872, 992
HinP1I GCGC 2 cut(s) 872, 992
HincII GTYRAC 2 cut(s) 1279, 1300
HindII GTYRAC 2 cut(s) 1279, 1300
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 7 cut(s) 436, 452, 632, 743, 772, 1046, 1576
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 1 cut(s) 1739
HphI GGTGA 2 cut(s) 98, 1737
Hpy166II GTNNAC 4 cut(s) 148, 1235, 1279, 1300
Hpy188I TCNGA 6 cut(s) 208, 373, 457, 777, 1095, 1446
Hpy188III TCNNGA 8 cut(s) 140, 272, 285, 500, 740, 769, 1202, 1710
Hpy8I GTNNAC 4 cut(s) 148, 1235, 1279, 1300
HpyAV CCTTC 4 cut(s) 934, 1102, 1531, 1608
HpyCH4III ACNGT 7 cut(s) 333, 559, 599, 604, 694, 807, 856
HpyCH4IV ACGT 2 cut(s) 1267, 1339
HpyF10VI GCNNNNNNNGC 8 cut(s) 61, 127, 314, 538, 727, 733, 967, 1262
HpyF3I CTNAG 6 cut(s) 428, 697, 894, 1068, 1121, 1403
HpySE526I ACGT 2 cut(s) 1267, 1339
Hsp92II CATG 4 cut(s) 566, 818, 1087, 1402
HspAI GCGC 2 cut(s) 872, 992
Ksp22I TGATCA 2 cut(s) 916, 1090
KspAI GTTAAC 1 cut(s) 1279
LmnI GCTCC 3 cut(s) 967, 1459, 1663
Lsp1109I GCAGC 7 cut(s) 39, 182, 516, 714, 761, 1003, 1681
LweI GCATC 3 cut(s) 1621, 1681, 1706
MaeI CTAG 6 cut(s) 621, 704, 1385, 1503, 1509, 1647
MaeII ACGT 2 cut(s) 1267, 1339
MaeIII GTNAC 8 cut(s) 4, 358, 604, 817, 824, 850, 1071, 1727
MflI RGATCY 3 cut(s) 502, 877, 1642
MhlI GDGCHC 4 cut(s) 187, 557, 868, 1237
MlyI GAGTC 5 cut(s) 430, 461, 626, 752, 1570
MmeI TCCRAC 1 cut(s) 244
MroXI GAANNNNTTC 2 cut(s) 1451, 1717
MseI TTAA 3 cut(s) 954, 1278, 1596
MspI CCGG 1 cut(s) 1739
MspR9I CCNGG 3 cut(s) 679, 861, 1457
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 3 cut(s) 679, 861, 1457
MwoI GCNNNNNNNGC 8 cut(s) 61, 127, 314, 538, 727, 733, 967, 1262
NlaIII CATG 4 cut(s) 566, 818, 1087, 1402
NlaIV GGNNCC 5 cut(s) 419, 865, 866, 1567, 1644
NmuCI GTSAC 3 cut(s) 4, 850, 1727
PaeR7I CTCGAG 1 cut(s) 987
PaqCI CACCTGC 1 cut(s) 47
PctI GAATGC 1 cut(s) 117
PdmI GAANNNNTTC 2 cut(s) 1451, 1717
PfeI GAWTC 2 cut(s) 772, 1046
PfoI TCCNGGA 1 cut(s) 1455
PkrI GCNGC 8 cut(s) 54, 172, 310, 531, 729, 751, 1018, 1696
PleI GAGTC 5 cut(s) 430, 460, 626, 751, 1570
PpsI GAGTC 5 cut(s) 430, 460, 626, 751, 1570
Ppu21I YACGTR 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 1547
Psp5II RGGWCCY 1 cut(s) 1547
Psp6I CCWGG 3 cut(s) 677, 859, 1455
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 3 cut(s) 677, 859, 1455
PspN4I GGNNCC 5 cut(s) 419, 865, 866, 1567, 1644
PspOMI GGGCCC 1 cut(s) 864
PspPI GGNCC 4 cut(s) 864, 865, 1547, 1565
PspPPI RGGWCCY 1 cut(s) 1547
PspXI VCTCGAGB 1 cut(s) 987
PstI CTGCAG 2 cut(s) 57, 66
PstNI CAGNNNCTG 3 cut(s) 61, 727, 860
PsuI RGATCY 3 cut(s) 502, 877, 1642
RsaI GTAC 4 cut(s) 378, 579, 601, 1217
RsaNI GTAC 4 cut(s) 377, 578, 600, 1216
SaqAI TTAA 3 cut(s) 954, 1278, 1596
SatI GCNGC 8 cut(s) 53, 171, 309, 530, 728, 750, 1017, 1695
Sau96I GGNCC 4 cut(s) 864, 865, 1547, 1565
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 5 cut(s) 430, 461, 626, 752, 1570
ScrFI CCNGG 3 cut(s) 679, 861, 1457
SduI GDGCHC 4 cut(s) 187, 557, 868, 1237
SfaNI GCATC 3 cut(s) 1621, 1681, 1706
SfcI CTRYAG 4 cut(s) 53, 62, 312, 595
Sfr274I CTCGAG 1 cut(s) 987
SinI GGWCC 2 cut(s) 1547, 1565
SlaI CTCGAG 1 cut(s) 987
SmlI CTYRAG 4 cut(s) 218, 971, 987, 1187
SmoI CTYRAG 4 cut(s) 218, 971, 987, 1187
SsiI CCGC 3 cut(s) 42, 308, 1429
SspMI CTAG 6 cut(s) 621, 704, 1385, 1503, 1509, 1647
StyD4I CCNGG 3 cut(s) 677, 859, 1455
TaaI ACNGT 7 cut(s) 333, 559, 599, 604, 694, 807, 856
TaiI ACGT 2 cut(s) 1270, 1342
TaqI TCGA 4 cut(s) 900, 988, 1519, 1682
TatI WGTACW 3 cut(s) 376, 577, 599
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 2 cut(s) 772, 1046
Tru1I TTAA 3 cut(s) 954, 1278, 1596
Tru9I TTAA 3 cut(s) 954, 1278, 1596
TscAI CASTG 7 cut(s) 330, 436, 564, 1015, 1311, 1629, 1732
TseFI GTSAC 3 cut(s) 4, 850, 1727
TseI GCWGC 7 cut(s) 52, 170, 529, 727, 749, 1016, 1694
Tsp45I GTSAC 3 cut(s) 4, 850, 1727
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 7 cut(s) 330, 436, 564, 1015, 1311, 1629, 1732
VneI GTGCAC 1 cut(s) 1233
VpaK11BI GGWCC 2 cut(s) 1547, 1565
XapI RAATTY 4 cut(s) 69, 365, 545, 1284
XhoI CTCGAG 1 cut(s) 987
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 2 cut(s) 1451, 1717
XspI CTAG 6 cut(s) 621, 704, 1385, 1503, 1509, 1647
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.