PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 439 |
| AcsI | RAATTY | 2 cut(s) | 296, 503 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 376 |
| AflIII | ACRYGT | 2 cut(s) | 137, 187 |
| AgsI | TTSAA | 3 cut(s) | 183, 211, 508 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 191 |
| AluBI | AGCT | 3 cut(s) | 262, 362, 389 |
| AluI | AGCT | 3 cut(s) | 262, 362, 389 |
| Alw26I | GTCTC | 3 cut(s) | 32, 43, 71 |
| AlwI | GGATC | 1 cut(s) | 439 |
| AoxI | GGCC | 1 cut(s) | 317 |
| ApeKI | GCWGC | 1 cut(s) | 123 |
| ApoI | RAATTY | 2 cut(s) | 296, 503 |
| ArsI | GACNNNNNNTTYG | 1 cut(s) | 35 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 311 |
| AspS9I | GGNCC | 1 cut(s) | 317 |
| AsuHPI | GGTGA | 2 cut(s) | 337, 505 |
| BbvI | GCAGC | 1 cut(s) | 110 |
| BccI | CCATC | 1 cut(s) | 110 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 449, 483 |
| BcoDI | GTCTC | 3 cut(s) | 32, 43, 71 |
| BfaI | CTAG | 2 cut(s) | 117, 517 |
| BisI | GCNGC | 1 cut(s) | 124 |
| BlsI | GCNGC | 1 cut(s) | 125 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 191 |
| BmgT120I | GGNCC | 1 cut(s) | 317 |
| BmsI | GCATC | 1 cut(s) | 135 |
| BsaJI | CCNNGG | 1 cut(s) | 265 |
| BsaWI | WCCGGW | 1 cut(s) | 155 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 376 |
| BseDI | CCNNGG | 1 cut(s) | 265 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 376 |
| BseMII | CTCAG | 1 cut(s) | 83 |
| BseRI | GAGGAG | 1 cut(s) | 511 |
| BseXI | GCAGC | 1 cut(s) | 110 |
| BsgI | GTGCAG | 1 cut(s) | 184 |
| Bsh1285I | CGRYCG | 1 cut(s) | 156 |
| BshFI | GGCC | 1 cut(s) | 319 |
| BsiEI | CGRYCG | 1 cut(s) | 156 |
| BsiSI | CCGG | 2 cut(s) | 156, 320 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 376 |
| BsmAI | GTCTC | 3 cut(s) | 32, 43, 71 |
| BsmBI | CGTCTC | 1 cut(s) | 71 |
| BsnI | GGCC | 1 cut(s) | 319 |
| Bsp143I | GATC | 1 cut(s) | 431 |
| BspANI | GGCC | 1 cut(s) | 319 |
| BspCNI | CTCAG | 1 cut(s) | 84 |
| BspPI | GGATC | 1 cut(s) | 439 |
| BssECI | CCNNGG | 1 cut(s) | 265 |
| BssMI | GATC | 1 cut(s) | 431 |
| BssT1I | CCWWGG | 1 cut(s) | 265 |
| Bst6I | CTCTTC | 1 cut(s) | 30 |
| BstC8I | GCNNGC | 1 cut(s) | 360 |
| BstDEI | CTNAG | 1 cut(s) | 92 |
| BstKTI | GATC | 1 cut(s) | 434 |
| BstMAI | GTCTC | 3 cut(s) | 32, 43, 71 |
| BstMBI | GATC | 1 cut(s) | 431 |
| BstMCI | CGRYCG | 1 cut(s) | 156 |
| BstNSI | RCATGY | 4 cut(s) | 141, 191, 255, 421 |
| BstV1I | GCAGC | 1 cut(s) | 110 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 490 |
| BsuRI | GGCC | 1 cut(s) | 319 |
| Cac8I | GCNNGC | 1 cut(s) | 360 |
| Cfr13I | GGNCC | 1 cut(s) | 317 |
| CviAII | CATG | 6 cut(s) | 138, 188, 252, 277, 418, 472 |
| CviJI | RGCY | 7 cut(s) | 5, 123, 262, 319, 358, 362, 389 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 123, 262, 319, 358, 362, 389 |
| DdeI | CTNAG | 1 cut(s) | 92 |
| DpnI | GATC | 1 cut(s) | 433 |
| DpnII | GATC | 1 cut(s) | 431 |
| DriI | GACNNNNNGTC | 1 cut(s) | 191 |
| Eam1104I | CTCTTC | 1 cut(s) | 30 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 191 |
| EarI | CTCTTC | 1 cut(s) | 30 |
| Eco130I | CCWWGG | 1 cut(s) | 265 |
| EcoT14I | CCWWGG | 1 cut(s) | 265 |
| ErhI | CCWWGG | 1 cut(s) | 265 |
| Esp3I | CGTCTC | 1 cut(s) | 71 |
| FaeI | CATG | 6 cut(s) | 141, 191, 255, 280, 421, 475 |
| FatI | CATG | 6 cut(s) | 137, 187, 251, 276, 417, 471 |
| Fnu4HI | GCNGC | 1 cut(s) | 124 |
| Fsp4HI | GCNGC | 1 cut(s) | 124 |
| FspBI | CTAG | 2 cut(s) | 117, 517 |
| GluI | GCNGC | 1 cut(s) | 124 |
| HaeIII | GGCC | 1 cut(s) | 319 |
| HapII | CCGG | 2 cut(s) | 156, 320 |
| Hin1II | CATG | 6 cut(s) | 141, 191, 255, 280, 421, 475 |
| HinfI | GANTC | 2 cut(s) | 18, 455 |
| HpaII | CCGG | 2 cut(s) | 156, 320 |
| HphI | GGTGA | 2 cut(s) | 337, 505 |
| Hpy188I | TCNGA | 3 cut(s) | 17, 153, 436 |
| Hpy188III | TCNNGA | 1 cut(s) | 183 |
| HpyAV | CCTTC | 2 cut(s) | 205, 322 |
| HpyCH4V | TGCA | 6 cut(s) | 88, 126, 165, 255, 338, 421 |
| HpyF3I | CTNAG | 1 cut(s) | 92 |
| Hsp92II | CATG | 6 cut(s) | 141, 191, 255, 280, 421, 475 |
| Kzo9I | GATC | 1 cut(s) | 431 |
| LmnI | GCTCC | 3 cut(s) | 267, 386, 498 |
| LpnPI | CCDG | 4 cut(s) | 169, 333, 362, 372 |
| Lsp1109I | GCAGC | 1 cut(s) | 110 |
| LweI | GCATC | 1 cut(s) | 135 |
| MaeI | CTAG | 2 cut(s) | 117, 517 |
| MalI | GATC | 1 cut(s) | 433 |
| MboI | GATC | 1 cut(s) | 431 |
| MboII | GAAGA | 3 cut(s) | 47, 171, 240 |
| MluCI | AATT | 3 cut(s) | 296, 476, 503 |
| MlyI | GAGTC | 1 cut(s) | 27 |
| MmeI | TCCRAC | 1 cut(s) | 176 |
| MnlI | CCTC | 6 cut(s) | 68, 136, 316, 359, 373, 489 |
| MroXI | GAANNNNTTC | 1 cut(s) | 311 |
| MseI | TTAA | 1 cut(s) | 479 |
| MspI | CCGG | 2 cut(s) | 156, 320 |
| NdeII | GATC | 1 cut(s) | 431 |
| NlaIII | CATG | 6 cut(s) | 141, 191, 255, 280, 421, 475 |
| NspI | RCATGY | 4 cut(s) | 141, 191, 255, 421 |
| PciI | ACATGT | 2 cut(s) | 137, 187 |
| PdmI | GAANNNNTTC | 1 cut(s) | 311 |
| PfeI | GAWTC | 1 cut(s) | 455 |
| PkrI | GCNGC | 1 cut(s) | 125 |
| PleI | GAGTC | 1 cut(s) | 26 |
| PpsI | GAGTC | 1 cut(s) | 26 |
| PscI | ACATGT | 2 cut(s) | 137, 187 |
| PspPI | GGNCC | 1 cut(s) | 317 |
| SaqAI | TTAA | 1 cut(s) | 479 |
| SatI | GCNGC | 1 cut(s) | 124 |
| Sau3AI | GATC | 1 cut(s) | 431 |
| Sau96I | GGNCC | 1 cut(s) | 317 |
| SchI | GAGTC | 1 cut(s) | 27 |
| SetI | ASST | 4 cut(s) | 223, 264, 364, 391 |
| SfaNI | GCATC | 1 cut(s) | 135 |
| Sse9I | AATT | 3 cut(s) | 296, 476, 503 |
| SspMI | CTAG | 2 cut(s) | 117, 517 |
| StyI | CCWWGG | 1 cut(s) | 265 |
| TaqI | TCGA | 1 cut(s) | 351 |
| TasI | AATT | 3 cut(s) | 296, 476, 503 |
| TfiI | GAWTC | 1 cut(s) | 455 |
| Tru1I | TTAA | 1 cut(s) | 479 |
| Tru9I | TTAA | 1 cut(s) | 479 |
| TseI | GCWGC | 1 cut(s) | 123 |
| TspGWI | ACGGA | 1 cut(s) | 95 |
| XapI | RAATTY | 2 cut(s) | 296, 503 |
| XceI | RCATGY | 4 cut(s) | 141, 191, 255, 421 |
| XmnI | GAANNNNTTC | 1 cut(s) | 311 |
| XspI | CTAG | 2 cut(s) | 117, 517 |