Rh1AG297000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000269
Physical Location & Seq
Reverse (-)
52838630 .. 52842156
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG297000.1

Sequence Viewer

Length: 2457 bp
ATGCCGCCGAAGGTCTACACAAAGAAGGCACCCAAAAGGGCGAAACCGAGCAGCTCCGGTAACTCTGATGGAGGTGCCCGAAAGAGTTCTCGAATGGCGGCCCCTAAACCGAGCAACTCCACAACGATCTCCGATGGAGGCCCTCGAAAGAGTACTCGAATCGCCGCCTCTAAAGCATCGGCTTTGGCGACGGTCTCTGAGGTCTCAAATCAAAATTCTCCAATTTTGGAGTCCGCAAAGAAAGCTTCTGTTAACGGCACCGAAGCGGTGGTTGAGGTACCTAAACTGAAACCTACTTCCGATGAAGCGGTAATTGAGGCACCGAAACCTACTTCCAATGATAAGCGGTTGCAAAAAGAGAAGGCACCAAAGGATTTAGAGTTGTCTGCCTCACCTGCAGAAGTCAAAGAGATTGAGTGCAGGAACATTCAACACAAACAAATCAATCCCCCAAAACTAAACCATGATTTTCGAGTTGAGATATCTCAAAAATGGAAAGAGCTTCCAGTTGAAGAGAAGGAGCAGTCTTTGGTTGCAGATTTTAGCGATGAGAAAAAGGCAGCCTGTTCAGCAATTGGATTTGATGTGTTGTTGAAGATGTCAGGAAGGAAAATTCACCACTCTATGTTGCGTGTCCTTGTGGACTCCGTAGACCCCACTGCATGTACTATAACTATGCACGGAAAGGAGATTGAAATGGATGCAAGTGACTTTGAAAATGTGATGGGGTTGAAGAATGCTGGGTCCGAGGTCGACTTTAAAGGTTCCACTAATGATCACCCTGAGTTGATGGCAATAATAAACTCCTTGTGTGGGAAGGATAAGAAGATCAGTTTAAGGGACGTGCAGAACTACTTGAAGGATACAGAAGAAGTTGACAACAAGTTCAAGCGCCTATTTGTGTTGTTCACAATGAGCACCATCCTTAGCCCATCTGCCTCACTGACGATACCAAAGAAGTGGCTGCTGGCCCTGAAGGACACTGGCCTAATTAGCTCTTTAAACTGGGCTGATTACTCATTCAAATGTTTGATGGAGGCTATCGTCAGTTTCAAAAAAGACTCTCAGTCCTATTGTAGCGGTCGTGTCCTGTTTCTGCAATTGTTCTATTTTGATTGTGTTTCACATGGGAAGATCATTGTGGACAAGTCCTTATACCCAGTTGAAGCTTGGGGGGACCATGAAATGGGCAAACTGCTCAAATGGGTGAACAAGCAAGGTGGTTTGCTGGATGAAAATGTTCTTGTGGCAAAGGTTGGTGACCGTGTTGGTAGTGGAGATCAGGCCAAAGCATATGGGGTGATTAAGAAAGAAATGTCTGACTTTGCTGCTAAAGTGAATGGGTTGGAAAATAATATGTGCAATCTGAGAGATGATATGATGGGAAAGATGGACAAAGTCTTGAGTATGCTGAACACTCTGACTGCACAAAATGTGAATGTCAATCAGAACCTTCAAACTGATGGCTTAGCAGAAAAAGTAAGCCCCAAGAAAGTTTCAAAAAAGAAAGCTGGAGAGCAACATGAGGTACATGGGGAAGACTTGCAACCAAAAAAGAAGACCTCCTTGATTTGCCTATCTGACTCAACATCTGTAGTCAGCCCACTGAAAAGTATGAAGAAAGTTGATGCCAACCCCACGACCAAGAGTAACATAGAGAAGATTGGTAACTTCAAAGTCAAGGGAGATCGGGAGGAGGCTGACAAAGGAGTGCTCAGGTTTGTTTATGATGTGTCAGCTGAAGAGAAGGAGACCATTGTGGATAGTGAAAATGTCTTCACTAGTCGAAAAGCAGTTTGGTCACTTAAACCTAACGGATGGGTTGAAGATGATATTATCAATTTGTTTAGTGACTATCTATTTCTAGGACGCTTCCCAAGCAATAGATGTTGTTACTTCACAACATACTTTTGTCAAAAGATGAAAAGTTACAATGACAAAGGCATGTGTGGAGATTCGGCTAGGGCAGTTTCGAAGGGTTTGGATGTAAAGAGATTTGAGAATGGCATACCTGAGTGCAAGAAGTTGCAAACACTGGACATTATCTACCACCATGTGGAGTTCAAAGTCAAGAATGTTGTGAAGAAGTTTGCTGACTTCCATGTATTCGAGCCGGCTCAATGCCCTAAACAAGAGGGCGGTTCTGACTATGGGGTGTATGTGATCAAACACATGCAGTGTTATGGATCTGAGTGGTGGCATCAGTTTAACTCAGAAGAGGTACGCATGGATTTAACCATTGAATTGGTAAGGAACAAATTCAATGAGTGCTGGAAATGGATCGAGGAAGCCATCAAAACTCGGCCACCACCAATATGCTCTACACCTAAGACGGCACAAAGGGAAACTAAAGCAACACCGAAGAAAGGGACCGTGAAGAACCCTATCGTCCAACCACCTTGCACTAGAGCTCAATCCAAGAGAGGTGTGGCATCAAATGTCCGTCGTAGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

818

Amino Acids

91.35

Weight (kDa)

9.07

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1985 PF09331 222 - 346 1e-06 Domain of unknown function (DUF1985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29141 FvH4_1g29142 FvH4_2g04882 FvH4_2g04883 FvH4_2g07612 FvH4_3g26550 FvH4_3g26551 FvH4_3g31781 FvH4_3g31810 FvH4_3g31812 FvH4_4g08580 FvH4_4g08581 FvH4_4g08649 FvH4_4g08910 FvH4_4g10124 FvH4_4g10125 FvH4_4g28760 FvH4_5g16042 FvH4_5g24070 FvH4_5g30680 FvH4_5g37871 FvH4_5g37872 FvH4_7g03531 FvH4_7g12891 FvH4_7g12892 FvH4_7g12893
rosa_chinensis RchiOBHm_Chr7g0204351
rosa_laevigata RLG00000001882 RLG00000002339 RLG00000004189 RLG00000009211 RLG00000009212 RLG00000013650 RLG00000019919 RLG00000019920 RLG00000019921 RLG00000020177 RLG00000020178 RLG00000023368 RLG00000028208 RLG00000029086 RLG00000029688 RLG00000032551 RLG00000034335 RLG00000034807
rosa_multiflora Rmu_sc0000308.1_g000017 Rmu_sc0000308.1_g000018 Rmu_sc0003422.1_g000004 Rmu_sc0003776.1_g000026 Rmu_sc0005399.1_g000010 Rmu_sc0005399.1_g000012 Rmu_sc0009924.1_g000004
rosa_roxburghii Rroxscaffold_1G00053720 Rroxscaffold_1G00053730 Rroxscaffold_1G00054020 Rroxscaffold_2G00133470 Rroxscaffold_2G00133480 Rroxscaffold_3G00229920 Rroxscaffold_3G00229930 Rroxscaffold_3G00229940 Rroxscaffold_3G00245890 Rroxscaffold_3G00245900 Rroxscaffold_4G00317860 Rroxscaffold_5G00346620 Rroxscaffold_6G00396640 Rroxscaffold_6G00396930 Rroxscaffold_6G00396940 Rroxscaffold_6G00401510 Rroxscaffold_7G00201920 Rroxscaffold_7G00201930 Rroxscaffold_7G00201940
rosa_rugosa Rorug01G0116700 Rorug01G0116700 Rorug04G0072900 Rorug04G0073000 Rorug04G0112000 Rorug07G0258800
rosa_samantha Rh1AG123800 Rh1AG297000 Rh1DG057400 Rh1DG057500 Rh1DG129000 Rh2BG298000 Rh2BG471200 Rh2BG471300 Rh2BG471400 Rh2BG621400 Rh3CG245500 Rh3CG245600 Rh4DG157200 Rh4DG157300 Rh4DG157400 Rh4DG157500 Rh4DG157600 Rh4DG157700 Rh5DG402200 Rh6AG050900 Rh6AG051000 Rh6AG088400 Rh6AG088500 Rh6AG088600 Rh6AG088700 Rh6AG139800 Rh6AG139900 Rh6AG140000 Rh6CG077200 Rh6CG077300 Rh6CG077400 Rh6CG077500 Rh7CG225300 Rh7CG225400 Rh7CG225500 Rh7CG225600 Rh7CG267500 Rh7CG267600 Rh7CG267800 Rh7CG267900 Rh7CG338700 Rh7CG338800 Rh7CG338900 Rh7CG430900 Rh7CG431000 Rh7CG431100 Rh7DG219700 Rh7DG219800 Rh7DG219900 Rh7DG220000 Rh7DG257600 Rh7DG271500 Rh7DG367700 Rh7DG408600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
Acc36I ACCTGC 1 cut(s) 403
Acc65I GGTACC 1 cut(s) 277
AccB1I GGYRCC 6 cut(s) 28, 74, 257, 277, 319, 364
AccB7I CCANNNNNTGG 2 cut(s) 1186, 2056
AccI GTMKAC 3 cut(s) 15, 651, 753
AciI CCGC 9 cut(s) 5, 98, 165, 234, 266, 308, 346, 1080, 2139
AclWI GGATC 2 cut(s) 2194, 2288
AcoI YGGCCR 1 cut(s) 2303
AcsI RAATTY 3 cut(s) 214, 612, 2258
AcuI CTGAAG 2 cut(s) 995, 1761
AdeI CACNNNGTG 1 cut(s) 2056
AfaI GTAC 5 cut(s) 154, 279, 667, 1530, 2223
AfiI CCNNNNNNNGG 4 cut(s) 813, 1186, 2056, 2366
AhdI GACNNNNNGTC 1 cut(s) 1066
AhlI ACTAGT 1 cut(s) 1781
AjiI CACGTC 1 cut(s) 844
AjuI GAANNNNNNNTTGG 2 cut(s) 1780, 1812
AluBI AGCT 8 cut(s) 54, 245, 502, 996, 1169, 1511, 1739, 2411
AluI AGCT 8 cut(s) 54, 245, 502, 996, 1169, 1511, 1739, 2411
Alw21I GWGCWC 3 cut(s) 920, 1716, 2413
Alw26I GTCTC 3 cut(s) 199, 208, 1745
AlwI GGATC 2 cut(s) 2194, 2288
AoxI GGCC 6 cut(s) 99, 139, 969, 985, 1284, 2303
ApeKI GCWGC 4 cut(s) 51, 560, 964, 1328
ApoI RAATTY 3 cut(s) 214, 612, 2258
Asp700I GAANNNNTTC 3 cut(s) 85, 1239, 2258
Asp718I GGTACC 1 cut(s) 277
AspLEI GCGC 1 cut(s) 894
AspS9I GGNCC 6 cut(s) 100, 140, 744, 970, 1177, 2370
AsuHPI GGTGA 6 cut(s) 384, 608, 770, 1219, 1271, 1312
AsuII TTCGAA 1 cut(s) 1973
AvaII GGWCC 3 cut(s) 744, 1177, 2370
BaeGI GKGCMC 1 cut(s) 79
BanI GGYRCC 6 cut(s) 28, 74, 257, 277, 319, 364
BanII GRGCYC 1 cut(s) 2413
BbsI GAAGAC 3 cut(s) 1545, 1565, 1768
Bbv12I GWGCWC 3 cut(s) 920, 1716, 2413
BbvI GCAGC 4 cut(s) 63, 572, 951, 1315
BceAI ACGGC 2 cut(s) 271, 2349
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 2 cut(s) 775, 2163
BcoDI GTCTC 3 cut(s) 199, 208, 1745
BcuI ACTAGT 1 cut(s) 1781
BfaI CTAG 4 cut(s) 1782, 1865, 1962, 2406
BfmI CTRYAG 2 cut(s) 396, 1593
BfoI RGCGCY 1 cut(s) 895
BfuAI ACCTGC 1 cut(s) 403
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 7 cut(s) 5, 52, 99, 165, 561, 965, 1329
BlpI GCTNAGC 1 cut(s) 1468
BlsI GCNGC 7 cut(s) 6, 53, 100, 166, 562, 966, 1330
BmcAI AGTACT 1 cut(s) 154
Bme18I GGWCC 3 cut(s) 744, 1177, 2370
BmeRI GACNNNNNGTC 1 cut(s) 1066
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 6 cut(s) 100, 140, 744, 970, 1177, 2370
BmrI ACTGGG 2 cut(s) 1015, 1154
BmsI GCATC 5 cut(s) 185, 691, 1618, 2209, 2441
BmuI ACTGGG 2 cut(s) 1015, 1154
BpiI GAAGAC 3 cut(s) 1545, 1565, 1768
BpmI CTGGAG 1 cut(s) 1533
Bpu10I CCTNAGC 2 cut(s) 926, 1715
Bpu1102I GCTNAGC 1 cut(s) 1468
Bpu14I TTCGAA 1 cut(s) 1973
BpuEI CTTGAG 1 cut(s) 1423
BsaI GGTCTC 3 cut(s) 199, 208, 1745
BsaJI CCNNGG 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 4 cut(s) 813, 1186, 2056, 2366
Bse118I RCCGGY 1 cut(s) 2113
Bse1I ACTGG 5 cut(s) 506, 988, 1010, 1160, 2040
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 5 cut(s) 706, 921, 1237, 1823, 1990
BseLI CCNNNNNNNGG 4 cut(s) 813, 1186, 2056, 2366
BseMII CTCAG 8 cut(s) 189, 774, 1079, 1358, 1729, 2004, 2181, 2226
BseNI ACTGG 5 cut(s) 506, 988, 1010, 1160, 2040
BseRI GAGGAG 1 cut(s) 1709
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 4 cut(s) 63, 572, 951, 1315
BseYI CCCAGC 1 cut(s) 740
BsgI GTGCAG 3 cut(s) 439, 866, 1410
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 6 cut(s) 101, 141, 971, 987, 1286, 2305
BshNI GGYRCC 6 cut(s) 28, 74, 257, 277, 319, 364
BsiEI CGRYCG 1 cut(s) 1084
BsiHKAI GWGCWC 3 cut(s) 920, 1716, 2413
BsiSI CCGG 2 cut(s) 57, 2114
BslFI GGGAC 3 cut(s) 854, 1190, 2383
BslI CCNNNNNNNGG 4 cut(s) 813, 1186, 2056, 2366
BsmAI GTCTC 3 cut(s) 199, 208, 1745
BsmFI GGGAC 3 cut(s) 854, 1190, 2383
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 6 cut(s) 101, 141, 971, 987, 1286, 2305
Bso31I GGTCTC 3 cut(s) 199, 208, 1745
Bsp119I TTCGAA 1 cut(s) 1973
Bsp1286I GDGCHC 4 cut(s) 79, 920, 1716, 2413
Bsp143I GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
Bsp1720I GCTNAGC 1 cut(s) 1468
BspACI CCGC 9 cut(s) 5, 98, 165, 234, 266, 308, 346, 1080, 2139
BspANI GGCC 6 cut(s) 101, 141, 971, 987, 1286, 2305
BspCNI CTCAG 8 cut(s) 190, 775, 1078, 1359, 1728, 2005, 2182, 2225
BspMAI CTGCAG 1 cut(s) 400
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 2 cut(s) 2194, 2288
BspT104I TTCGAA 1 cut(s) 1973
BspT107I GGYRCC 6 cut(s) 28, 74, 257, 277, 319, 364
BspTNI GGTCTC 3 cut(s) 199, 208, 1745
BsrFI RCCGGY 1 cut(s) 2113
BsrI ACTGG 5 cut(s) 506, 988, 1010, 1160, 2040
BssAI RCCGGY 1 cut(s) 2113
BssECI CCNNGG 1 cut(s) 747
BssMI GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
Bst4CI ACNGT 3 cut(s) 193, 1265, 2374
Bst6I CTCTTC 3 cut(s) 507, 1737, 2211
BstBI TTCGAA 1 cut(s) 1973
BstC8I GCNNGC 2 cut(s) 969, 2115
BstEII GGTNACC 1 cut(s) 1259
BstF5I GGATG 5 cut(s) 706, 921, 1237, 1823, 1990
BstH2I RGCGCY 1 cut(s) 895
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 9 cut(s) 129, 778, 831, 1137, 1282, 1690, 2166, 2189, 2283
BstMAI GTCTC 3 cut(s) 199, 208, 1745
BstMBI GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 6 cut(s) 173, 242, 395, 569, 993, 1878
BstNSI RCATGY 3 cut(s) 666, 1948, 2176
BstPI GGTNACC 1 cut(s) 1259
BstSFI CTRYAG 2 cut(s) 396, 1593
BstSLI GKGCMC 1 cut(s) 79
BstV1I GCAGC 4 cut(s) 63, 572, 951, 1315
BstV2I GAAGAC 3 cut(s) 1545, 1565, 1768
BstX2I RGATCY 1 cut(s) 2186
BstXI CCANNNNNNTGG 2 cut(s) 960, 2245
BstYI RGATCY 1 cut(s) 2186
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 6 cut(s) 101, 141, 971, 987, 1286, 2305
BtgZI GCGATG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 5 cut(s) 706, 921, 1237, 1823, 1990
BtsI GCAGTG 2 cut(s) 657, 2183
BtsIMutI CAGTG 6 cut(s) 657, 941, 981, 1604, 2033, 2183
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 969, 2115
CfoI GCGC 1 cut(s) 894
Cfr10I RCCGGY 1 cut(s) 2113
Cfr13I GGNCC 6 cut(s) 100, 140, 744, 970, 1177, 2370
CseI GACGC 1 cut(s) 1878
Csp6I GTAC 5 cut(s) 153, 278, 666, 1529, 2222
CspCI CAANNNNNGTGG 2 cut(s) 1201, 1236
CviQI GTAC 5 cut(s) 153, 278, 666, 1529, 2222
DpnI GATC 9 cut(s) 128, 777, 830, 1136, 1281, 1689, 2165, 2188, 2282
DpnII GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
DraI TTTAAA 2 cut(s) 760, 1002
DraIII CACNNNGTG 1 cut(s) 2056
DriI GACNNNNNGTC 1 cut(s) 1066
EaeI YGGCCR 1 cut(s) 2303
Eam1104I CTCTTC 3 cut(s) 507, 1737, 2211
Eam1105I GACNNNNNGTC 1 cut(s) 1066
EarI CTCTTC 3 cut(s) 507, 1737, 2211
Ecl136II GAGCTC 1 cut(s) 2411
Eco24I GRGCYC 1 cut(s) 2413
Eco31I GGTCTC 3 cut(s) 199, 208, 1745
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 3 cut(s) 744, 1177, 2370
Eco53kI GAGCTC 1 cut(s) 2411
Eco57I CTGAAG 2 cut(s) 995, 1761
Eco91I GGTNACC 1 cut(s) 1259
EcoICRI GAGCTC 1 cut(s) 2411
EcoO109I RGGNCCY 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 1259
EcoRV GATATC 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 2413
FalI AAGNNNNNCTT 2 cut(s) 1550, 1582
FaqI GGGAC 3 cut(s) 854, 1190, 2383
FauNDI CATATG 1 cut(s) 1294
FbaI TGATCA 2 cut(s) 775, 2163
FblI GTMKAC 3 cut(s) 15, 651, 753
Fnu4HI GCNGC 7 cut(s) 5, 52, 99, 165, 561, 965, 1329
FokI GGATG 5 cut(s) 713, 908, 1244, 1830, 1997
FriOI GRGCYC 1 cut(s) 2413
Fsp4HI GCNGC 7 cut(s) 5, 52, 99, 165, 561, 965, 1329
FspBI CTAG 4 cut(s) 1782, 1865, 1962, 2406
GlaI GCGC 1 cut(s) 893
GluI GCNGC 7 cut(s) 5, 52, 99, 165, 561, 965, 1329
GsaI CCCAGC 1 cut(s) 744
GsuI CTGGAG 1 cut(s) 1533
HaeII RGCGCY 1 cut(s) 895
HaeIII GGCC 6 cut(s) 101, 141, 971, 987, 1286, 2305
HapII CCGG 2 cut(s) 57, 2114
HgaI GACGC 1 cut(s) 1878
HhaI GCGC 1 cut(s) 894
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HincII GTYRAC 3 cut(s) 253, 754, 877
HindII GTYRAC 3 cut(s) 253, 754, 877
HindIII AAGCTT 2 cut(s) 243, 1167
HinfI GANTC 6 cut(s) 159, 230, 644, 1061, 1583, 1955
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 2 cut(s) 57, 2114
HphI GGTGA 6 cut(s) 384, 608, 770, 1219, 1271, 1312
Hpy166II GTNNAC 9 cut(s) 16, 253, 643, 652, 754, 877, 909, 1144, 1210
Hpy188III TCNNGA 5 cut(s) 90, 603, 1402, 1691, 2071
Hpy8I GTNNAC 9 cut(s) 16, 253, 643, 652, 754, 877, 909, 1144, 1210
Hpy99I CGWCG 2 cut(s) 193, 2448
HpyCH4III ACNGT 3 cut(s) 193, 1265, 2374
HpyCH4IV ACGT 1 cut(s) 843
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 242, 395, 569, 993, 1878
HpySE526I ACGT 1 cut(s) 843
HspAI GCGC 1 cut(s) 892
KpnI GGTACC 1 cut(s) 281
KroI GCCGGC 1 cut(s) 2113
KroNI GCCGGC 1 cut(s) 2115
Ksp22I TGATCA 2 cut(s) 775, 2163
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
LmnI GCTCC 2 cut(s) 59, 520
Lsp1109I GCAGC 4 cut(s) 63, 572, 951, 1315
LweI GCATC 5 cut(s) 185, 691, 1618, 2209, 2441
MaeI CTAG 4 cut(s) 1782, 1865, 1962, 2406
MaeII ACGT 1 cut(s) 843
MaeIII GTNAC 9 cut(s) 59, 707, 1259, 1649, 1667, 1800, 1850, 1892, 1928
MalI GATC 9 cut(s) 128, 777, 830, 1136, 1281, 1689, 2165, 2188, 2282
MboI GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
MfeI CAATTG 2 cut(s) 573, 1100
MflI RGATCY 1 cut(s) 2186
MhlI GDGCHC 4 cut(s) 79, 920, 1716, 2413
MlyI GAGTC 4 cut(s) 239, 638, 1055, 1577
MmeI TCCRAC 2 cut(s) 1326, 2416
MroNI GCCGGC 1 cut(s) 2113
MroXI GAANNNNTTC 3 cut(s) 85, 1239, 2258
MseI TTAA 8 cut(s) 252, 759, 836, 1001, 1305, 1806, 2208, 2234
MslI CAYNNNNRTG 2 cut(s) 1024, 2314
MspA1I CMGCKG 1 cut(s) 1739
MspI CCGG 2 cut(s) 57, 2114
MunI CAATTG 2 cut(s) 573, 1100
Mva1269I GAATGC 1 cut(s) 742
MwoI GCNNNNNNNGC 6 cut(s) 173, 242, 395, 569, 993, 1878
NaeI GCCGGC 1 cut(s) 2115
NdeI CATATG 1 cut(s) 1294
NdeII GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
NgoMIV GCCGGC 1 cut(s) 2113
NmeAIII GCCGAG 1 cut(s) 2281
NmuCI GTSAC 4 cut(s) 707, 1259, 1800, 1850
NspI RCATGY 3 cut(s) 666, 1948, 2176
NspV TTCGAA 1 cut(s) 1973
PaqCI CACCTGC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PctI GAATGC 1 cut(s) 742
PdiI GCCGGC 1 cut(s) 2115
PdmI GAANNNNTTC 3 cut(s) 85, 1239, 2258
PfeI GAWTC 2 cut(s) 159, 1955
PflFI GACNNNGTC 1 cut(s) 1397
PflMI CCANNNNNTGG 2 cut(s) 1186, 2056
PkrI GCNGC 7 cut(s) 6, 53, 100, 166, 562, 966, 1330
PleI GAGTC 4 cut(s) 238, 638, 1055, 1577
PpsI GAGTC 4 cut(s) 238, 638, 1055, 1577
Psp124BI GAGCTC 1 cut(s) 2413
PspEI GGTNACC 1 cut(s) 1259
PspFI CCCAGC 1 cut(s) 740
PspPI GGNCC 6 cut(s) 100, 140, 744, 970, 1177, 2370
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 2186
PsyI GACNNNGTC 1 cut(s) 1397
PvuII CAGCTG 1 cut(s) 1739
RsaI GTAC 5 cut(s) 154, 279, 667, 1530, 2223
RsaNI GTAC 5 cut(s) 153, 278, 666, 1529, 2222
RseI CAYNNNNRTG 2 cut(s) 1024, 2314
SacI GAGCTC 1 cut(s) 2413
SalI GTCGAC 1 cut(s) 752
SaqAI TTAA 8 cut(s) 252, 759, 836, 1001, 1305, 1806, 2208, 2234
SatI GCNGC 7 cut(s) 5, 52, 99, 165, 561, 965, 1329
Sau3AI GATC 9 cut(s) 126, 775, 828, 1134, 1279, 1687, 2163, 2186, 2280
Sau96I GGNCC 6 cut(s) 100, 140, 744, 970, 1177, 2370
ScaI AGTACT 1 cut(s) 154
SchI GAGTC 4 cut(s) 239, 638, 1055, 1577
SduI GDGCHC 4 cut(s) 79, 920, 1716, 2413
SfaNI GCATC 5 cut(s) 185, 691, 1618, 2209, 2441
SfcI CTRYAG 2 cut(s) 396, 1593
SfuI TTCGAA 1 cut(s) 1973
SinI GGWCC 3 cut(s) 744, 1177, 2370
SmiMI CAYNNNNRTG 2 cut(s) 1024, 2314
SmlI CTYRAG 1 cut(s) 1402
SmoI CTYRAG 1 cut(s) 1402
SpeI ACTAGT 1 cut(s) 1781
SsiI CCGC 9 cut(s) 5, 98, 165, 234, 266, 308, 346, 1080, 2139
SspMI CTAG 4 cut(s) 1782, 1865, 1962, 2406
SstI GAGCTC 1 cut(s) 2413
TaaI ACNGT 3 cut(s) 193, 1265, 2374
TaiI ACGT 1 cut(s) 846
TaqI TCGA 9 cut(s) 91, 145, 157, 472, 753, 1786, 1973, 2109, 2283
TatI WGTACW 2 cut(s) 152, 665
TauI GCSGC 3 cut(s) 7, 101, 167
TfiI GAWTC 2 cut(s) 159, 1955
Tru1I TTAA 8 cut(s) 252, 759, 836, 1001, 1305, 1806, 2208, 2234
Tru9I TTAA 8 cut(s) 252, 759, 836, 1001, 1305, 1806, 2208, 2234
TscAI CASTG 6 cut(s) 664, 948, 988, 1611, 2040, 2183
TseFI GTSAC 4 cut(s) 707, 1259, 1800, 1850
TseI GCWGC 4 cut(s) 51, 560, 964, 1328
Tsp45I GTSAC 4 cut(s) 707, 1259, 1800, 1850
TspDTI ATGAA 5 cut(s) 318, 1197, 1248, 1631, 1937
TspGWI ACGGA 4 cut(s) 637, 696, 1830, 2432
TspRI CASTG 6 cut(s) 664, 948, 988, 1611, 2040, 2183
Tth111I GACNNNGTC 1 cut(s) 1397
Van91I CCANNNNNTGG 2 cut(s) 1186, 2056
VpaK11BI GGWCC 3 cut(s) 744, 1177, 2370
XapI RAATTY 3 cut(s) 214, 612, 2258
XceI RCATGY 3 cut(s) 666, 1948, 2176
XcmI CCANNNNNNNNNTGG 2 cut(s) 1167, 2425
XmiI GTMKAC 3 cut(s) 15, 651, 753
XmnI GAANNNNTTC 3 cut(s) 85, 1239, 2258
XspI CTAG 4 cut(s) 1782, 1865, 1962, 2406
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.