Rorug04G0073000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000269
Physical Location & Seq
Reverse (-)
11355371 .. 11359101
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0073000.1

Sequence Viewer

Length: 2436 bp
ATGTACTCAAATTCGAAAGCTGATTCATGGCGCTTAAGGTTGTGCTCGTCACTCGTAGTCATCACTATGATCACAGTTGATGTAGTACATGGAGATTCGCTAGACACAGACATGGAGGTCCTGCTAAGTCTTAAGGAATTTATGCAACAACATAATAGAATAAACCAAGGCCAATACTTAAAATGGAACCAGAGCAACAACCCATGTGAATGGCCTAGAATTTCATGCAATGATGACAATAGAGTCAGTGCAATTGATCTCTCCTATAGCAAAATCTCCGGTGAGGTTTTCGGAAACTTCTCTGCCCTGACAGCACTGTCTCACCTTGACCTATCCATGAACACTTTGGGTGGAGCTATCCCAGAGGACCTGAGTCAATGCCAGAGCCTCAAACACCTCAACCTGTCACATAACATTCTTGAAGGTGAGATGAAATTGCAAGGGTTTAGTCAATTAGAGGTTCTTGATTTGGCTGTCAACAGGTTTTATGGGGACATGAATATTAGATTCCCTGGAATTTGCAAAAACTTAGTAGTTGCCAATGTTTCAACGAACGGCTTTACTAGTAGGATTGACCAGTGCTTTGATGAATGCTTGAATTTGCAGTACTTGGATTTGAGCTCAAACAGTTTTAGTGGTGATTTGTGGGTCGGGTTTACAAGGCTTAGGGAGTTTTCAGTGTCCGAAAATTATCTCAGTGGGGCTATTTTGCCTTCTATTTTCAATGGTAGTTGTAGCTTGGTGAATTTGGAACTGTCAGAAAATGAATTTTCTGGTGAACTTGCAAAAGGGATTTCAAATTGCCAGAATTTGGTTACATTGAATCTCTGGGGAAACAAATTTACAGGGCCAATTCCATCTGAGATTGGAAACATTTCGAGTCTCAAATCATTGTTCTTGGGAAATAATAGTTTTTCTAGAGTGATCCCAGAATCCCTTCTGGATTTGAGCAGCTTGGTGTCCTTGGATTTAAGCAGAAACAATTTTGGAGGGGATATACAAGATATATTTGGAAGATTCACGCAGGTGAAGTTTCTTGTGCTGCACTCAAATTCATACACTGGCGGTGTATATTCATCTGGAATTCTTAAGCTACCAAATGTTTCTCTATTAGACCTCAGCTACAACAATTTTACAGGTTCTTTACCAGTTGAAATTGCTCAAATGCCAAGCTTGAAGTATTTGTTCCTTGCTTATAATCACTTCAATGGAACAATACCACCAGAGTATGGAAATTTGTCACACCTCCAAGCACTAGACCTTTCCTTCAACAGTCTATCTGGGTCAATCCCCCCCACCATAGGAAACTTGAAACCTCTTATGTTTTTTATGCTTGCAAATAATTTGCTAACTGGTCCTATTCCCAAGGAGTTGGGGAATTGTACTAGCTTGTTGTGGCTGAACCTTTCAAACAACAAGCTTAGTGGGACAATCCCAACTGAATTGAAGAATATAGGGTCAGATGTTGGTCCAACATTTGAATCAAACAGTCGGGACAAGCATCAGATCATAGCTGGATCCAGGGAGTGCTTAGTAATGAAGAGATGGATTCCAGCAGACTACCCCCCATTCAGTTTTGTGTATAGAATCCTCACTGGGAAGAGTTGTCAAAACATATGGGATCAGCTCCTCAAGGGAAATGGCCTTTTTCCCATATGTGTTTCTGGTTCTGCAATAAGGACATTACAAATCTCCGGTTACATTCAACTGAGTGGGAATCAACTTTCAGGTCAAGTCCCTCCAGAGATTGGTAAGATGCACAATTTTAGTATGTTGCACTTGGCTTTTAATAAATTAAAGGGACAACTCCCTGAAGAGATTGGACAAATGCCACTTATAGTGTTTAACCTCACTAAGAACAGTTTTTCGGGCCAAATCCCGATGGAAATCGGAAACATCAAGTGCATGCAGAATTTGGATTTGTCCTACAACAACTTTTCTGGTACCTTCCCTCGCAGCTTAAACCGATTGACTGAGCTAAATAATGAGGGTATCCTTCCGAGATTTATTGACAACTCCACAGAGCATGAGGATGAGGTTATAAGACATGGATTGTACATCAGCATGGGACTTGGATTTGTGGTTGGATTTTGGGGAGTTTGTGGGAGCTTGATTTTCATCAGGTCATGGAGATACTCATACTATGGTTTCTTGAATCTTCTACATGACTGGCTTTATGTGAGGGTGGCTGTGATCAGGCGGAAACTAAAGGACATGCTTAGAACAGGGCCGGTCCTGAGGCAAGTAATGCATGCCTCCCTTCAGCTGAAGAAGGCCATTGACAATCATCTGGAGAAGCTTTACAGTATAGCCGGAGTGCTACATAAGCTGCAGTGTACCATGTCTTCAATTTTTTCTCCTTTATGGAGCCATATGTGCTTAACTTCAAACTTGTACTATCTTAAAACTCAAAAGAAACCAGCTGTAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

811

Amino Acids

90.61

Weight (kDa)

6.81

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 84 - 138 3e-06 Leucine rich repeat
LRR_8 PF13855 270 - 329 8.4e-08 Leucine rich repeat
LRR_8 PF13855 344 - 402 6.9e-07 Leucine rich repeat
LRR_14 PF23598 371 - 484 3.9e-10 Leucine-rich repeat region
LRR_8 PF13855 414 - 474 5.8e-06 Leucine rich repeat
LRR_14 PF23598 567 - 662 3.8e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29141 FvH4_1g29142 FvH4_2g04882 FvH4_2g04883 FvH4_2g07612 FvH4_3g26550 FvH4_3g26551 FvH4_3g31781 FvH4_3g31810 FvH4_3g31812 FvH4_4g08580 FvH4_4g08581 FvH4_4g08649 FvH4_4g08910 FvH4_4g10124 FvH4_4g10125 FvH4_4g28760 FvH4_5g16042 FvH4_5g24070 FvH4_5g30680 FvH4_5g37871 FvH4_5g37872 FvH4_7g03531 FvH4_7g12891 FvH4_7g12892 FvH4_7g12893
rosa_chinensis RchiOBHm_Chr7g0204351
rosa_laevigata RLG00000001882 RLG00000002339 RLG00000004189 RLG00000009211 RLG00000009212 RLG00000013650 RLG00000019919 RLG00000019920 RLG00000019921 RLG00000020177 RLG00000020178 RLG00000023368 RLG00000028208 RLG00000029086 RLG00000029688 RLG00000032551 RLG00000034335 RLG00000034807
rosa_multiflora Rmu_sc0000308.1_g000017 Rmu_sc0000308.1_g000018 Rmu_sc0003422.1_g000004 Rmu_sc0003776.1_g000026 Rmu_sc0005399.1_g000010 Rmu_sc0005399.1_g000012 Rmu_sc0009924.1_g000004
rosa_roxburghii Rroxscaffold_1G00053720 Rroxscaffold_1G00053730 Rroxscaffold_1G00054020 Rroxscaffold_2G00133470 Rroxscaffold_2G00133480 Rroxscaffold_3G00229920 Rroxscaffold_3G00229930 Rroxscaffold_3G00229940 Rroxscaffold_3G00245890 Rroxscaffold_3G00245900 Rroxscaffold_4G00317860 Rroxscaffold_5G00346620 Rroxscaffold_6G00396640 Rroxscaffold_6G00396930 Rroxscaffold_6G00396940 Rroxscaffold_6G00401510 Rroxscaffold_7G00201920 Rroxscaffold_7G00201930 Rroxscaffold_7G00201940
rosa_rugosa Rorug01G0116700 Rorug01G0116700 Rorug04G0072900 Rorug04G0073000 Rorug04G0112000 Rorug07G0258800
rosa_samantha Rh1AG123800 Rh1AG297000 Rh1DG057400 Rh1DG057500 Rh1DG129000 Rh2BG298000 Rh2BG471200 Rh2BG471300 Rh2BG471400 Rh2BG621400 Rh3CG245500 Rh3CG245600 Rh4DG157200 Rh4DG157300 Rh4DG157400 Rh4DG157500 Rh4DG157600 Rh4DG157700 Rh5DG402200 Rh6AG050900 Rh6AG051000 Rh6AG088400 Rh6AG088500 Rh6AG088600 Rh6AG088700 Rh6AG139800 Rh6AG139900 Rh6AG140000 Rh6CG077200 Rh6CG077300 Rh6CG077400 Rh6CG077500 Rh7CG225300 Rh7CG225400 Rh7CG225500 Rh7CG225600 Rh7CG267500 Rh7CG267600 Rh7CG267800 Rh7CG267900 Rh7CG338700 Rh7CG338800 Rh7CG338900 Rh7CG430900 Rh7CG431000 Rh7CG431100 Rh7DG219700 Rh7DG219800 Rh7DG219900 Rh7DG220000 Rh7DG257600 Rh7DG271500 Rh7DG367700 Rh7DG408600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1197, 2042
AarI CACCTGC 1 cut(s) 1015
AasI GACNNNNNNGTC 3 cut(s) 116, 242, 316
Acc36I ACCTGC 1 cut(s) 1015
Acc65I GGTACC 1 cut(s) 1943
AccB1I GGYRCC 1 cut(s) 1943
AccB7I CCANNNNNTGG 3 cut(s) 811, 1229, 1748
AciI CCGC 2 cut(s) 1065, 2200
AclWI GGATC 4 cut(s) 919, 1512, 1525, 1629
AcuI CTGAAG 3 cut(s) 1833, 2246, 2288
AfaI GTAC 8 cut(s) 5, 87, 608, 1384, 1945, 2057, 2338, 2396
AfiI CCNNNNNNNGG 4 cut(s) 811, 1229, 1301, 1748
AflII CTTAAG 3 cut(s) 34, 131, 1088
AhlI ACTAGT 1 cut(s) 563
AjnI CCWGG 2 cut(s) 511, 1520
AleI CACNNNNGTG 1 cut(s) 1025
Alw21I GWGCWC 2 cut(s) 47, 623
Alw26I GTCTC 2 cut(s) 324, 887
AlwI GGATC 4 cut(s) 919, 1512, 1525, 1629
AoxI GGCC 7 cut(s) 169, 212, 848, 1642, 1870, 2228, 2274
ApeKI GCWGC 4 cut(s) 951, 1042, 1956, 2329
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
Asp700I GAANNNNTTC 2 cut(s) 874, 936
Asp718I GGTACC 1 cut(s) 1943
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 8 cut(s) 118, 367, 848, 1355, 1469, 1870, 2228, 2233
AsuHPI GGTGA 7 cut(s) 293, 314, 437, 650, 754, 788, 1039
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 5 cut(s) 118, 367, 1355, 1469, 2233
AxyI CCTNAGG 1 cut(s) 2237
BamHI GGATCC 1 cut(s) 1517
BanI GGYRCC 1 cut(s) 1943
BanII GRGCYC 1 cut(s) 623
BarI GAAGNNNNNNTAC 2 cut(s) 2329, 2361
BbsI GAAGAC 1 cut(s) 2337
Bbv12I GWGCWC 2 cut(s) 47, 623
BbvCI CCTCAGC 1 cut(s) 1118
BbvI GCAGC 4 cut(s) 963, 1029, 1968, 2316
BccI CCATC 3 cut(s) 865, 1539, 1876
BceAI ACGGC 1 cut(s) 571
BciT130I CCWGG 2 cut(s) 513, 1522
BciVI GTATCC 1 cut(s) 2003
BclI TGATCA 2 cut(s) 69, 2193
BcoDI GTCTC 2 cut(s) 324, 887
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 6 cut(s) 101, 216, 564, 918, 1256, 1386
BfmI CTRYAG 3 cut(s) 265, 2330, 2424
BfoI RGCGCY 1 cut(s) 34
BfrI CTTAAG 3 cut(s) 34, 131, 1088
BfuAI ACCTGC 1 cut(s) 1015
BfuI GTATCC 1 cut(s) 2003
BisI GCNGC 4 cut(s) 952, 1043, 1957, 2330
BlsI GCNGC 4 cut(s) 953, 1044, 1958, 2331
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 2 cut(s) 513, 1522
Bme18I GGWCC 5 cut(s) 118, 367, 1355, 1469, 2233
BmgT120I GGNCC 8 cut(s) 118, 367, 848, 1355, 1469, 1870, 2228, 2233
BmiI GGNNCC 4 cut(s) 188, 1519, 1945, 2369
BmrFI CCNGG 2 cut(s) 513, 1522
BmrI ACTGGG 1 cut(s) 1605
BmsI GCATC 2 cut(s) 1510, 1746
BmuI ACTGGG 1 cut(s) 1605
BoxI GACNNNNGTC 1 cut(s) 372
BpiI GAAGAC 1 cut(s) 2337
BpmI CTGGAG 2 cut(s) 1725, 2312
Bpu10I CCTNAGC 2 cut(s) 665, 1118
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 1616
BsaBI GATNNNNATC 2 cut(s) 1886, 2117
BsaJI CCNNGG 5 cut(s) 166, 511, 963, 1365, 1521
BsaWI WCCGGW 2 cut(s) 278, 1694
Bsc4I CCNNNNNNNGG 4 cut(s) 811, 1229, 1301, 1748
Bse118I RCCGGY 1 cut(s) 2230
Bse1I ACTGG 6 cut(s) 577, 1066, 1148, 1357, 1600, 2174
Bse21I CCTNAGG 1 cut(s) 2237
Bse3DI GCAATG 1 cut(s) 235
Bse8I GATNNNNATC 2 cut(s) 1886, 2117
BseBI CCWGG 2 cut(s) 513, 1522
BseDI CCNNGG 5 cut(s) 166, 511, 963, 1365, 1521
BseGI GGATG 1 cut(s) 2038
BseJI GATNNNNATC 2 cut(s) 1886, 2117
BseLI CCNNNNNNNGG 4 cut(s) 811, 1229, 1301, 1748
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 7 cut(s) 362, 709, 852, 1132, 1700, 1965, 2228
BseNI ACTGG 6 cut(s) 577, 1066, 1148, 1357, 1600, 2174
BseRI GAGGAG 1 cut(s) 1619
BseXI GCAGC 4 cut(s) 963, 1029, 1968, 2316
BsgI GTGCAG 1 cut(s) 1028
BshFI GGCC 7 cut(s) 171, 214, 850, 1644, 1872, 2230, 2276
BshNI GGYRCC 1 cut(s) 1943
BsiHKAI GWGCWC 2 cut(s) 47, 623
BsiSI CCGG 4 cut(s) 279, 1695, 2231, 2313
BslFI GGGAC 6 cut(s) 506, 1441, 1508, 1721, 1815, 2082
BslI CCNNNNNNNGG 4 cut(s) 811, 1229, 1301, 1748
BsmAI GTCTC 2 cut(s) 324, 887
BsmFI GGGAC 6 cut(s) 506, 1441, 1508, 1721, 1815, 2082
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 7 cut(s) 171, 214, 850, 1644, 1872, 2230, 2276
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 47, 623
Bsp1407I TGTACA 1 cut(s) 2055
Bsp143I GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
BspACI CCGC 2 cut(s) 1065, 2200
BspANI GGCC 7 cut(s) 171, 214, 850, 1644, 1872, 2230, 2276
BspCNI CTCAG 7 cut(s) 363, 708, 853, 1131, 1701, 1966, 2229
BspLI GGNNCC 4 cut(s) 188, 1519, 1945, 2369
BspMAI CTGCAG 1 cut(s) 2334
BspMI ACCTGC 1 cut(s) 1015
BspPI GGATC 4 cut(s) 919, 1512, 1525, 1629
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 1943
BspTI CTTAAG 3 cut(s) 34, 131, 1088
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 2230
BsrGI TGTACA 1 cut(s) 2055
BsrI ACTGG 6 cut(s) 577, 1066, 1148, 1357, 1600, 2174
BssAI RCCGGY 1 cut(s) 2230
BssECI CCNNGG 5 cut(s) 166, 511, 963, 1365, 1521
BssMI GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
BssT1I CCWWGG 3 cut(s) 166, 963, 1365
Bst2UI CCWGG 2 cut(s) 513, 1522
Bst4CI ACNGT 8 cut(s) 76, 318, 629, 756, 1274, 1490, 1862, 2306
Bst6I CTCTTC 3 cut(s) 1535, 1595, 1809
BstAFI CTTAAG 3 cut(s) 34, 131, 1088
BstAPI GCANNNNNTGC 1 cut(s) 2248
BstAUI TGTACA 1 cut(s) 2055
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 1335, 1907, 2253
BstF5I GGATG 1 cut(s) 2038
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 7 cut(s) 72, 259, 927, 1509, 1520, 1624, 2196
BstMAI GTCTC 2 cut(s) 324, 887
BstMBI GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
BstMWI GCNNNNNNNGC 4 cut(s) 311, 2248, 2326, 2376
BstNI CCWGG 2 cut(s) 513, 1522
BstNSI RCATGY 3 cut(s) 1909, 2218, 2255
BstPAI GACNNNNGTC 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 511, 1520
BstSFI CTRYAG 3 cut(s) 265, 2330, 2424
BstV1I GCAGC 4 cut(s) 963, 1029, 1968, 2316
BstV2I GAAGAC 1 cut(s) 2337
BstX2I RGATCY 1 cut(s) 1517
BstXI CCANNNNNNTGG 2 cut(s) 210, 1372
BstYI RGATCY 1 cut(s) 1517
Bsu36I CCTNAGG 1 cut(s) 2237
BsuI GTATCC 1 cut(s) 2003
BsuRI GGCC 7 cut(s) 171, 214, 850, 1644, 1872, 2230, 2276
BtsCI GGATG 1 cut(s) 2038
BtsI GCAGTG 1 cut(s) 2339
BtsIMutI CAGTG 8 cut(s) 253, 314, 584, 684, 703, 1059, 1593, 2339
BveI ACCTGC 1 cut(s) 1015
Cac8I GCNNGC 3 cut(s) 1335, 1907, 2253
CfoI GCGC 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 2230
Cfr13I GGNCC 8 cut(s) 118, 367, 848, 1355, 1469, 1870, 2228, 2233
Csp6I GTAC 8 cut(s) 4, 86, 607, 1383, 1944, 2056, 2337, 2395
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviQI GTAC 8 cut(s) 4, 86, 607, 1383, 1944, 2056, 2337, 2395
DpnI GATC 7 cut(s) 71, 258, 926, 1508, 1519, 1623, 2195
DpnII GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
DrdI GACNNNNNNGTC 3 cut(s) 116, 242, 316
DseDI GACNNNNNNGTC 3 cut(s) 116, 242, 316
Eam1104I CTCTTC 3 cut(s) 1535, 1595, 1809
EarI CTCTTC 3 cut(s) 1535, 1595, 1809
EciI GGCGGA 1 cut(s) 2215
Ecl136II GAGCTC 1 cut(s) 621
Eco130I CCWWGG 3 cut(s) 166, 963, 1365
Eco24I GRGCYC 1 cut(s) 623
Eco47I GGWCC 5 cut(s) 118, 367, 1355, 1469, 2233
Eco53kI GAGCTC 1 cut(s) 621
Eco57I CTGAAG 3 cut(s) 1833, 2246, 2288
Eco81I CCTNAGG 1 cut(s) 2237
EcoICRI GAGCTC 1 cut(s) 621
EcoO109I RGGNCCY 2 cut(s) 118, 367
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 2 cut(s) 511, 1520
EcoT14I CCWWGG 3 cut(s) 166, 963, 1365
EcoT22I ATGCAT 1 cut(s) 2253
EcoT38I GRGCYC 1 cut(s) 623
ErhI CCWWGG 3 cut(s) 166, 963, 1365
FaqI GGGAC 6 cut(s) 506, 1441, 1508, 1721, 1815, 2082
FauNDI CATATG 3 cut(s) 1616, 1655, 2373
FbaI TGATCA 2 cut(s) 69, 2193
Fnu4HI GCNGC 4 cut(s) 952, 1043, 1957, 2330
FokI GGATG 1 cut(s) 2045
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 4 cut(s) 952, 1043, 1957, 2330
FspBI CTAG 6 cut(s) 101, 216, 564, 918, 1256, 1386
GlaI GCGC 1 cut(s) 32
GluI GCNGC 4 cut(s) 952, 1043, 1957, 2330
GsuI CTGGAG 2 cut(s) 1725, 2312
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 7 cut(s) 171, 214, 850, 1644, 1872, 2230, 2276
HapII CCGG 4 cut(s) 279, 1695, 2231, 2313
HhaI GCGC 1 cut(s) 33
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 3 cut(s) 1171, 1418, 2297
HpaII CCGG 4 cut(s) 279, 1695, 2231, 2313
HphI GGTGA 7 cut(s) 293, 314, 437, 650, 754, 788, 1039
Hpy166II GTNNAC 4 cut(s) 478, 657, 779, 2337
Hpy188I TCNGA 8 cut(s) 293, 685, 760, 862, 1462, 1506, 1892, 2001
Hpy8I GTNNAC 4 cut(s) 478, 657, 779, 2337
HpyAV CCTTC 8 cut(s) 416, 723, 947, 1276, 1957, 2006, 2266, 2270
HpyCH4III ACNGT 8 cut(s) 76, 318, 629, 756, 1274, 1490, 1862, 2306
HpyF10VI GCNNNNNNNGC 4 cut(s) 311, 2248, 2326, 2376
HspAI GCGC 1 cut(s) 31
KpnI GGTACC 1 cut(s) 1947
Ksp22I TGATCA 2 cut(s) 69, 2193
Kzo9I GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
LmnI GCTCC 4 cut(s) 353, 1632, 2106, 2367
Lsp1109I GCAGC 4 cut(s) 963, 1029, 1968, 2316
LweI GCATC 2 cut(s) 1510, 1746
MaeI CTAG 6 cut(s) 101, 216, 564, 918, 1256, 1386
MaeIII GTNAC 5 cut(s) 48, 405, 814, 1239, 1697
MalI GATC 7 cut(s) 71, 258, 926, 1508, 1519, 1623, 2195
MboI GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
MboII GAAGA 8 cut(s) 1026, 1459, 1552, 1612, 1826, 2150, 2281, 2337
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 1517
MhlI GDGCHC 2 cut(s) 47, 623
MlyI GAGTC 3 cut(s) 252, 382, 889
MmeI TCCRAC 2 cut(s) 1496, 2065
Mph1103I ATGCAT 1 cut(s) 2253
MroXI GAANNNNTTC 2 cut(s) 874, 936
MslI CAYNNNNRTG 7 cut(s) 65, 110, 208, 1025, 1206, 2031, 2063
MspA1I CMGCKG 2 cut(s) 2266, 2423
MspCI CTTAAG 3 cut(s) 34, 131, 1088
MspI CCGG 4 cut(s) 279, 1695, 2231, 2313
MspR9I CCNGG 2 cut(s) 513, 1522
MunI CAATTG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 2 cut(s) 513, 1522
MwoI GCNNNNNNNGC 4 cut(s) 311, 2248, 2326, 2376
NdeI CATATG 3 cut(s) 1616, 1655, 2373
NdeII GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
NlaIV GGNNCC 4 cut(s) 188, 1519, 1945, 2369
NmuCI GTSAC 3 cut(s) 48, 405, 1239
NsiI ATGCAT 1 cut(s) 2253
NspI RCATGY 3 cut(s) 1909, 2218, 2255
NspV TTCGAA 1 cut(s) 14
OliI CACNNNNGTG 1 cut(s) 1025
PaeI GCATGC 2 cut(s) 1909, 2255
PaqCI CACCTGC 1 cut(s) 1015
PctI GAATGC 1 cut(s) 596
PdmI GAANNNNTTC 2 cut(s) 874, 936
PflMI CCANNNNNTGG 3 cut(s) 811, 1229, 1748
PkrI GCNGC 4 cut(s) 953, 1044, 1958, 2331
PleI GAGTC 3 cut(s) 251, 381, 888
PpsI GAGTC 3 cut(s) 251, 381, 888
PpuMI RGGWCCY 2 cut(s) 118, 367
PshAI GACNNNNGTC 1 cut(s) 372
PsiI TTATAA 2 cut(s) 1197, 2042
Psp124BI GAGCTC 1 cut(s) 623
Psp5II RGGWCCY 2 cut(s) 118, 367
Psp6I CCWGG 2 cut(s) 511, 1520
PspGI CCWGG 2 cut(s) 511, 1520
PspN4I GGNNCC 4 cut(s) 188, 1519, 1945, 2369
PspPI GGNCC 8 cut(s) 118, 367, 848, 1355, 1469, 1870, 2228, 2233
PspPPI RGGWCCY 2 cut(s) 118, 367
PstI CTGCAG 1 cut(s) 2334
PsuI RGATCY 1 cut(s) 1517
PvuII CAGCTG 2 cut(s) 2266, 2423
RsaI GTAC 8 cut(s) 5, 87, 608, 1384, 1945, 2057, 2338, 2396
RsaNI GTAC 8 cut(s) 4, 86, 607, 1383, 1944, 2056, 2337, 2395
RseI CAYNNNNRTG 7 cut(s) 65, 110, 208, 1025, 1206, 2031, 2063
SacI GAGCTC 1 cut(s) 623
SatI GCNGC 4 cut(s) 952, 1043, 1957, 2330
Sau3AI GATC 7 cut(s) 69, 256, 924, 1506, 1517, 1621, 2193
Sau96I GGNCC 8 cut(s) 118, 367, 848, 1355, 1469, 1870, 2228, 2233
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 3 cut(s) 252, 382, 889
ScrFI CCNGG 2 cut(s) 513, 1522
SduI GDGCHC 2 cut(s) 47, 623
SfaNI GCATC 2 cut(s) 1510, 1746
SfcI CTRYAG 3 cut(s) 265, 2330, 2424
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 5 cut(s) 118, 367, 1355, 1469, 2233
SmiMI CAYNNNNRTG 7 cut(s) 65, 110, 208, 1025, 1206, 2031, 2063
SmlI CTYRAG 4 cut(s) 34, 131, 1088, 1631
SmoI CTYRAG 4 cut(s) 34, 131, 1088, 1631
SpeI ACTAGT 1 cut(s) 563
SphI GCATGC 2 cut(s) 1909, 2255
SsiI CCGC 2 cut(s) 1065, 2200
SspI AATATT 1 cut(s) 502
SspMI CTAG 6 cut(s) 101, 216, 564, 918, 1256, 1386
SstI GAGCTC 1 cut(s) 623
StyD4I CCNGG 2 cut(s) 511, 1520
StyI CCWWGG 3 cut(s) 166, 963, 1365
TaaI ACNGT 8 cut(s) 76, 318, 629, 756, 1274, 1490, 1862, 2306
TaqI TCGA 2 cut(s) 14, 878
TatI WGTACW 6 cut(s) 3, 85, 606, 1382, 2055, 2394
TscAI CASTG 8 cut(s) 253, 321, 584, 684, 703, 1066, 1600, 2339
TseFI GTSAC 3 cut(s) 48, 405, 1239
TseI GCWGC 4 cut(s) 951, 1042, 1956, 2329
Tsp45I GTSAC 3 cut(s) 48, 405, 1239
TspRI CASTG 8 cut(s) 253, 321, 584, 684, 703, 1066, 1600, 2339
Van91I CCANNNNNTGG 3 cut(s) 811, 1229, 1748
Vha464I CTTAAG 3 cut(s) 34, 131, 1088
VpaK11BI GGWCC 5 cut(s) 118, 367, 1355, 1469, 2233
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 3 cut(s) 1909, 2218, 2255
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmnI GAANNNNTTC 2 cut(s) 874, 936
XspI CTAG 6 cut(s) 101, 216, 564, 918, 1256, 1386
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 2253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.