FvH4_4g10125

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000269
Physical Location & Seq
Reverse (-)
13020230 .. 13022077
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10125.t1

Sequence Viewer

Length: 1032 bp
ATGTATCAGAGCTCCACAAATCCGCGATATCAAAGACTGGAAGGAAAGCCGCAGAAGCTGCAAACGTTGTGGCTTGATAAATCGGCCTCTTCTACCGAAACGGATGACACCTCCAATCCATCGAAGAGGGCTCGACCATCGCCAACTGATGGTGTAGATGCCTCTGCCTCTGTTCCCGGATCGTCGTCTGAGGCTGCGCGCATGAACGATTCCCAGCGTGGACCTGTATCTTCACTGGAAGAGGGCCTGATTCCCAAGACGAGGTCGGAACGAGGGGGCGACGTGTGTCAACCAATAGAGGAAGGAGACGAAGACGAGGAGGATAATGAAGATCACCGTGAAGAGGGAGAAGATCATTTTGATGATGCGAAAGGGCAAGTGGCTGGCGACCGTGTGGTCTTCCGGAAGAGGAATAAAGTCACCGGGAAACAACTGAGTTTCTTCAACTATGTGAACCTACACCTAAAGTCAGGGAATGTGCCGAAGAACCAAGTTCACGAATTCAAGTTGAGCCTATCTCAGAAATGGAAGGAACTTCTGCCGGAAGAGAAGGCAAATTACGTCCACCCTGATAAAGAGGGTTACTCGTCAGCTTCTTATTCCGAGGGAAGTGCGCCAGCGGTCTACAAGGTAGACACTCGCACATCTCCGCACCGGTTCAAGAGTTTGATCGGAGAGTTCAATGAAGAGAAGTTAGCTGCTTGCAGGGAGCTCGGTTTTGGCCCTCTCTTGAAGTTGGCTATTACTAAGATGCACCTTTCCATTCTGAGGCTCATAGTCGATGCCACTAGCGCCTCAGATAAAAGTATAACTATGCATGGGACCAAGCTTCCACTCTCAGCCAATGATGTGGAGAATGTGATGGGTTTGAAGAATGGCGGATCCCCGGTTGTCTTTGAGGGGCATGTGAAAGATTATACGGAGTTGGAGGCCACTGCAAATGAGATGGCAGGGCCTGACCACAAGATCCAACTCGCCAAGGTCGAGGCGTACTTGAAGGAGACGCAATTGGTGGATGACAAATTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

344

Amino Acids

37.98

Weight (kDa)

5.58

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29141 FvH4_1g29142 FvH4_2g04882 FvH4_2g04883 FvH4_2g07612 FvH4_3g26550 FvH4_3g26551 FvH4_3g31781 FvH4_3g31810 FvH4_3g31812 FvH4_4g08580 FvH4_4g08581 FvH4_4g08649 FvH4_4g08910 FvH4_4g10124 FvH4_4g10125 FvH4_4g28760 FvH4_5g16042 FvH4_5g24070 FvH4_5g30680 FvH4_5g37871 FvH4_5g37872 FvH4_7g03531 FvH4_7g12891 FvH4_7g12892 FvH4_7g12893
rosa_chinensis RchiOBHm_Chr7g0204351
rosa_laevigata RLG00000001882 RLG00000002339 RLG00000004189 RLG00000009211 RLG00000009212 RLG00000013650 RLG00000019919 RLG00000019920 RLG00000019921 RLG00000020177 RLG00000020178 RLG00000023368 RLG00000028208 RLG00000029086 RLG00000029688 RLG00000032551 RLG00000034335 RLG00000034807
rosa_multiflora Rmu_sc0000308.1_g000017 Rmu_sc0000308.1_g000018 Rmu_sc0003422.1_g000004 Rmu_sc0003776.1_g000026 Rmu_sc0005399.1_g000010 Rmu_sc0005399.1_g000012 Rmu_sc0009924.1_g000004
rosa_roxburghii Rroxscaffold_1G00053720 Rroxscaffold_1G00053730 Rroxscaffold_1G00054020 Rroxscaffold_2G00133470 Rroxscaffold_2G00133480 Rroxscaffold_3G00229920 Rroxscaffold_3G00229930 Rroxscaffold_3G00229940 Rroxscaffold_3G00245890 Rroxscaffold_3G00245900 Rroxscaffold_4G00317860 Rroxscaffold_5G00346620 Rroxscaffold_6G00396640 Rroxscaffold_6G00396930 Rroxscaffold_6G00396940 Rroxscaffold_6G00401510 Rroxscaffold_7G00201920 Rroxscaffold_7G00201930 Rroxscaffold_7G00201940
rosa_rugosa Rorug01G0116700 Rorug01G0116700 Rorug04G0072900 Rorug04G0073000 Rorug04G0112000 Rorug07G0258800
rosa_samantha Rh1AG123800 Rh1AG297000 Rh1DG057400 Rh1DG057500 Rh1DG129000 Rh2BG298000 Rh2BG471200 Rh2BG471300 Rh2BG471400 Rh2BG621400 Rh3CG245500 Rh3CG245600 Rh4DG157200 Rh4DG157300 Rh4DG157400 Rh4DG157500 Rh4DG157600 Rh4DG157700 Rh5DG402200 Rh6AG050900 Rh6AG051000 Rh6AG088400 Rh6AG088500 Rh6AG088600 Rh6AG088700 Rh6AG139800 Rh6AG139900 Rh6AG140000 Rh6CG077200 Rh6CG077300 Rh6CG077400 Rh6CG077500 Rh7CG225300 Rh7CG225400 Rh7CG225500 Rh7CG225600 Rh7CG267500 Rh7CG267600 Rh7CG267800 Rh7CG267900 Rh7CG338700 Rh7CG338800 Rh7CG338900 Rh7CG430900 Rh7CG431000 Rh7CG431100 Rh7DG219700 Rh7DG219800 Rh7DG219900 Rh7DG220000 Rh7DG257600 Rh7DG271500 Rh7DG367700 Rh7DG408600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 149
AccI GTMKAC 2 cut(s) 624, 633
AccII CGCG 2 cut(s) 25, 199
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 5 cut(s) 23, 50, 620, 650, 879
AclI AACGTT 1 cut(s) 65
AclWI GGATC 4 cut(s) 187, 876, 889, 961
AcsI RAATTY 2 cut(s) 500, 1022
AfaI GTAC 1 cut(s) 992
AfiI CCNNNNNNNGG 4 cut(s) 149, 261, 343, 768
AflIII ACRYGT 1 cut(s) 282
AgeI ACCGGT 1 cut(s) 654
AgsI TTSAA 8 cut(s) 445, 505, 661, 682, 733, 871, 997, 1027
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 6 cut(s) 12, 58, 593, 698, 712, 829
AluI AGCT 6 cut(s) 12, 58, 593, 698, 712, 829
Alw21I GWGCWC 2 cut(s) 14, 714
Alw26I GTCTC 2 cut(s) 300, 995
AlwI GGATC 4 cut(s) 187, 876, 889, 961
AlwNI CAGNNNCTG 2 cut(s) 58, 956
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 5 cut(s) 84, 244, 721, 930, 953
ApeKI GCWGC 3 cut(s) 58, 194, 698
ApoI RAATTY 2 cut(s) 500, 1022
AsiGI ACCGGT 1 cut(s) 654
AspLEI GCGC 4 cut(s) 199, 201, 616, 794
AspS9I GGNCC 5 cut(s) 221, 244, 722, 822, 953
AsuC2I CCSGG 3 cut(s) 177, 424, 887
AsuHPI GGTGA 2 cut(s) 326, 412
AvaII GGWCC 2 cut(s) 221, 822
BamHI GGATCC 1 cut(s) 881
BanII GRGCYC 3 cut(s) 14, 133, 714
BarI GAAGNNNNNNTAC 2 cut(s) 542, 574
BbsI GAAGAC 2 cut(s) 318, 391
Bbv12I GWGCWC 2 cut(s) 14, 714
BbvI GCAGC 3 cut(s) 45, 181, 685
BccI CCATC 5 cut(s) 127, 143, 145, 856, 940
BcgI CGANNNNNNTGC 4 cut(s) 593, 627, 694, 728
BcnI CCSGG 3 cut(s) 177, 424, 887
BcoDI GTCTC 2 cut(s) 300, 995
BfaI CTAG 1 cut(s) 789
BfoI RGCGCY 1 cut(s) 795
BisI GCNGC 4 cut(s) 50, 59, 195, 699
BlsI GCNGC 4 cut(s) 51, 60, 196, 700
Bme1390I CCNGG 3 cut(s) 177, 424, 887
Bme18I GGWCC 2 cut(s) 221, 822
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 5 cut(s) 221, 244, 722, 822, 953
BmiI GGNNCC 2 cut(s) 823, 883
BmrFI CCNGG 3 cut(s) 177, 424, 887
BmsI GCATC 4 cut(s) 148, 355, 741, 772
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 318, 391
BplI GAGNNNNNCTC 4 cut(s) 502, 534, 569, 601
BpuMI CCSGG 3 cut(s) 177, 424, 887
BsaJI CCNNGG 3 cut(s) 603, 885, 978
BsaWI WCCGGW 2 cut(s) 402, 654
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 261, 343, 768
Bse118I RCCGGY 1 cut(s) 654
Bse1I ACTGG 2 cut(s) 42, 240
BseAI TCCGGA 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 603, 885, 978
BseGI GGATG 2 cut(s) 109, 1021
BseLI CCNNNNNNNGG 4 cut(s) 149, 261, 343, 768
BseMII CTCAG 6 cut(s) 180, 425, 533, 758, 810, 852
BseNI ACTGG 2 cut(s) 42, 240
BsePI GCGCGC 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 3 cut(s) 45, 181, 685
BseYI CCCAGC 1 cut(s) 213
Bsh1236I CGCG 2 cut(s) 25, 199
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 5 cut(s) 86, 246, 723, 932, 955
BshTI ACCGGT 1 cut(s) 654
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 2 cut(s) 14, 714
BsiSI CCGG 6 cut(s) 177, 403, 423, 542, 655, 887
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 4 cut(s) 149, 261, 343, 768
BsmAI GTCTC 2 cut(s) 300, 995
BsmBI CGTCTC 2 cut(s) 300, 995
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 5 cut(s) 86, 246, 723, 932, 955
Bsp1286I GDGCHC 3 cut(s) 14, 133, 714
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 6 cut(s) 179, 331, 352, 669, 881, 966
BspACI CCGC 5 cut(s) 23, 50, 620, 650, 879
BspANI GGCC 5 cut(s) 86, 246, 723, 932, 955
BspCNI CTCAG 6 cut(s) 181, 426, 532, 759, 809, 851
BspEI TCCGGA 1 cut(s) 402
BspFNI CGCG 2 cut(s) 25, 199
BspLI GGNNCC 2 cut(s) 823, 883
BspPI GGATC 4 cut(s) 187, 876, 889, 961
BsrFI RCCGGY 1 cut(s) 654
BsrI ACTGG 2 cut(s) 42, 240
BssAI RCCGGY 1 cut(s) 654
BssECI CCNNGG 3 cut(s) 603, 885, 978
BssHII GCGCGC 1 cut(s) 197
BssMI GATC 6 cut(s) 179, 331, 352, 669, 881, 966
BssT1I CCWWGG 1 cut(s) 978
Bst4CI ACNGT 2 cut(s) 338, 392
Bst6I CTCTTC 7 cut(s) 94, 119, 234, 336, 401, 540, 681
BstAPI GCANNNNNTGC 1 cut(s) 58
BstC8I GCNNGC 4 cut(s) 199, 385, 618, 703
BstDEI CTNAG 7 cut(s) 189, 434, 519, 747, 767, 796, 838
BstF5I GGATG 2 cut(s) 109, 1021
BstFNI CGCG 2 cut(s) 25, 199
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 4 cut(s) 199, 201, 616, 794
BstKTI GATC 6 cut(s) 182, 334, 355, 672, 884, 969
BstMAI GTCTC 2 cut(s) 300, 995
BstMBI GATC 6 cut(s) 179, 331, 352, 669, 881, 966
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 55, 58, 791
BstNSI RCATGY 1 cut(s) 908
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 3 cut(s) 175, 422, 885
BstUI CGCG 2 cut(s) 25, 199
BstV1I GCAGC 3 cut(s) 45, 181, 685
BstV2I GAAGAC 2 cut(s) 318, 391
BstX2I RGATCY 2 cut(s) 881, 966
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 2 cut(s) 881, 966
BsuRI GGCC 5 cut(s) 86, 246, 723, 932, 955
BtgZI GCGATG 1 cut(s) 123
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 2 cut(s) 109, 1021
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 2 cut(s) 233, 933
Cac8I GCNNGC 4 cut(s) 199, 385, 618, 703
CaiI CAGNNNCTG 2 cut(s) 58, 956
CfoI GCGC 4 cut(s) 199, 201, 616, 794
Cfr10I RCCGGY 1 cut(s) 654
Cfr13I GGNCC 5 cut(s) 221, 244, 722, 822, 953
CseI GACGC 1 cut(s) 1012
Csp6I GTAC 1 cut(s) 991
CspAI ACCGGT 1 cut(s) 654
CspCI CAANNNNNGTGG 2 cut(s) 50, 85
CviAII CATG 3 cut(s) 202, 818, 905
CviQI GTAC 1 cut(s) 991
DdeI CTNAG 7 cut(s) 189, 434, 519, 747, 767, 796, 838
DpnI GATC 6 cut(s) 181, 333, 354, 671, 883, 968
DpnII GATC 6 cut(s) 179, 331, 352, 669, 881, 966
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eam1104I CTCTTC 7 cut(s) 94, 119, 234, 336, 401, 540, 681
EarI CTCTTC 7 cut(s) 94, 119, 234, 336, 401, 540, 681
EciI GGCGGA 1 cut(s) 894
Ecl136II GAGCTC 2 cut(s) 12, 712
Eco130I CCWWGG 1 cut(s) 978
Eco24I GRGCYC 3 cut(s) 14, 133, 714
Eco32I GATATC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 221, 822
Eco53kI GAGCTC 2 cut(s) 12, 712
EcoICRI GAGCTC 2 cut(s) 12, 712
EcoO109I RGGNCCY 2 cut(s) 244, 953
EcoRI GAATTC 1 cut(s) 500
EcoRV GATATC 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 978
EcoT22I ATGCAT 1 cut(s) 819
EcoT38I GRGCYC 3 cut(s) 14, 133, 714
ErhI CCWWGG 1 cut(s) 978
Esp3I CGTCTC 2 cut(s) 300, 995
FaeI CATG 3 cut(s) 205, 821, 908
FaiI YATR 8 cut(s) 203, 450, 776, 809, 815, 819, 906, 918
FaqI GGGAC 1 cut(s) 835
FatI CATG 3 cut(s) 201, 817, 904
FblI GTMKAC 2 cut(s) 624, 633
Fnu4HI GCNGC 4 cut(s) 50, 59, 195, 699
FokI GGATG 2 cut(s) 116, 1028
FriOI GRGCYC 3 cut(s) 14, 133, 714
Fsp4HI GCNGC 4 cut(s) 50, 59, 195, 699
FspBI CTAG 1 cut(s) 789
GlaI GCGC 4 cut(s) 198, 200, 615, 793
GluI GCNGC 4 cut(s) 50, 59, 195, 699
GsaI CCCAGC 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 5 cut(s) 86, 246, 723, 932, 955
HapII CCGG 6 cut(s) 177, 403, 423, 542, 655, 887
HgaI GACGC 1 cut(s) 1012
HhaI GCGC 4 cut(s) 199, 201, 616, 794
Hin1II CATG 3 cut(s) 205, 821, 908
Hin6I GCGC 4 cut(s) 197, 199, 614, 792
HinP1I GCGC 4 cut(s) 197, 199, 614, 792
HincII GTYRAC 1 cut(s) 290
HindII GTYRAC 1 cut(s) 290
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 2 cut(s) 209, 250
HpaII CCGG 6 cut(s) 177, 403, 423, 542, 655, 887
HphI GGTGA 2 cut(s) 326, 412
Hpy166II GTNNAC 7 cut(s) 221, 290, 454, 496, 565, 625, 634
Hpy188I TCNGA 8 cut(s) 9, 190, 268, 522, 604, 674, 768, 799
Hpy188III TCNNGA 4 cut(s) 403, 497, 661, 730
Hpy8I GTNNAC 7 cut(s) 221, 290, 454, 496, 565, 625, 634
Hpy99I CGWCG 2 cut(s) 187, 284
HpyAV CCTTC 5 cut(s) 35, 296, 523, 544, 991
HpyCH4III ACNGT 2 cut(s) 338, 392
HpyCH4IV ACGT 3 cut(s) 65, 282, 561
HpyCH4V TGCA 5 cut(s) 61, 705, 754, 817, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 58, 791
HpyF3I CTNAG 7 cut(s) 189, 434, 519, 747, 767, 796, 838
HpySE526I ACGT 3 cut(s) 65, 282, 561
Hsp92II CATG 3 cut(s) 205, 821, 908
HspAI GCGC 4 cut(s) 197, 199, 614, 792
Kpn2I TCCGGA 1 cut(s) 402
Kzo9I GATC 6 cut(s) 179, 331, 352, 669, 881, 966
LmnI GCTCC 2 cut(s) 17, 709
Lsp1109I GCAGC 3 cut(s) 45, 181, 685
LweI GCATC 4 cut(s) 148, 355, 741, 772
MaeI CTAG 1 cut(s) 789
MaeII ACGT 3 cut(s) 65, 282, 561
MaeIII GTNAC 2 cut(s) 418, 581
MalI GATC 6 cut(s) 181, 333, 354, 671, 883, 968
MboI GATC 6 cut(s) 179, 331, 352, 669, 881, 966
MfeI CAATTG 1 cut(s) 1007
MflI RGATCY 2 cut(s) 881, 966
MhlI GDGCHC 3 cut(s) 14, 133, 714
MluCI AATT 4 cut(s) 500, 556, 1007, 1022
MmeI TCCRAC 3 cut(s) 246, 906, 994
Mph1103I ATGCAT 1 cut(s) 819
MroI TCCGGA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 6 cut(s) 177, 403, 423, 542, 655, 887
MspR9I CCNGG 3 cut(s) 177, 424, 887
MunI CAATTG 1 cut(s) 1007
MvnI CGCG 2 cut(s) 25, 199
MwoI GCNNNNNNNGC 3 cut(s) 55, 58, 791
NciI CCSGG 3 cut(s) 177, 424, 887
NdeII GATC 6 cut(s) 179, 331, 352, 669, 881, 966
NlaIII CATG 3 cut(s) 205, 821, 908
NlaIV GGNNCC 2 cut(s) 823, 883
NmuCI GTSAC 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 819
NspI RCATGY 1 cut(s) 908
PauI GCGCGC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 981
PfeI GAWTC 2 cut(s) 209, 250
PflFI GACNNNGTC 1 cut(s) 262
PflMI CCANNNNNTGG 1 cut(s) 149
PfoI TCCNGGA 1 cut(s) 175
PinAI ACCGGT 1 cut(s) 654
PkrI GCNGC 4 cut(s) 51, 60, 196, 700
PshAI GACNNNNGTC 1 cut(s) 285
Psp124BI GAGCTC 2 cut(s) 14, 714
Psp1406I AACGTT 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 823, 883
PspPI GGNCC 5 cut(s) 221, 244, 722, 822, 953
PstNI CAGNNNCTG 2 cut(s) 58, 956
PsuI RGATCY 2 cut(s) 881, 966
PsyI GACNNNGTC 1 cut(s) 262
PteI GCGCGC 1 cut(s) 197
RsaI GTAC 1 cut(s) 992
RsaNI GTAC 1 cut(s) 991
RseI CAYNNNNRTG 1 cut(s) 360
SacI GAGCTC 2 cut(s) 14, 714
SatI GCNGC 4 cut(s) 50, 59, 195, 699
Sau3AI GATC 6 cut(s) 179, 331, 352, 669, 881, 966
Sau96I GGNCC 5 cut(s) 221, 244, 722, 822, 953
ScrFI CCNGG 3 cut(s) 177, 424, 887
SduI GDGCHC 3 cut(s) 14, 133, 714
SfaNI GCATC 4 cut(s) 148, 355, 741, 772
SinI GGWCC 2 cut(s) 221, 822
SmiMI CAYNNNNRTG 1 cut(s) 360
Sse9I AATT 4 cut(s) 500, 556, 1007, 1022
SsiI CCGC 5 cut(s) 23, 50, 620, 650, 879
SspMI CTAG 1 cut(s) 789
SstI GAGCTC 2 cut(s) 14, 714
StyD4I CCNGG 3 cut(s) 175, 422, 885
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 2 cut(s) 338, 392
TaiI ACGT 3 cut(s) 68, 285, 564
TaqI TCGA 4 cut(s) 122, 133, 780, 984
TasI AATT 4 cut(s) 500, 556, 1007, 1022
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 2 cut(s) 209, 250
TscAI CASTG 2 cut(s) 240, 940
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 3 cut(s) 58, 194, 698
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 218, 342, 699
TspGWI ACGGA 2 cut(s) 116, 935
TspRI CASTG 2 cut(s) 240, 940
Tth111I GACNNNGTC 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 2 cut(s) 221, 822
XapI RAATTY 2 cut(s) 500, 1022
XceI RCATGY 1 cut(s) 908
XmiI GTMKAC 2 cut(s) 624, 633
XspI CTAG 1 cut(s) 789
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.