FvH4_5g30680

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000269
Physical Location & Seq
Reverse (-)
21589958 .. 21593551
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30680.t1

Sequence Viewer

Length: 2625 bp
ATGCCGCCGAAGAAGAAACCCCCGGTGAAAAAACAAACGAAAAAGTCGCCGCCGAAGAAGGCCCCGACGAAGAAGCCCAAGGCTGTCCAAGAGAGGAAAAGGGCTAATGCCTCGGACGCAGATGGAAGGGCGAGGAAGAGTTCCCGATTGTTGGGTATCGATCCACCAAGCTGCAGCTCCGACACTGTCTCTGATCCCCAAGTGCGATTAGTGGAGCAAGACAAAAAGAAACCGACAATTGAGAAGTTGTCAAATTCGGCTCCTGATTCCAGTGCCCCATGCTCTATGTTGGTTATTGCGAATGAAGCTGCGACGGAGGCGTCGAAACCCGAAATGGCCGGTGAGGAGGCAAAATCTAGTAAGGTTGCAAAGATGAGGTCTCCACTGAAGCCCAAGCTGAAGAAAAACTCCAACAAAGGGGGGATATTTTTAAATTACCTTGATTGTGTATTGATTTTGTTTCAATTCCTCTTGGAGTCTTCACAAGAATGGAGTACGTTTCCTCTCGAGAAAAAACAAAAGTACATTCCAGAGCCATCACATACCGCAGATGATGCTTCTTATGAGTCTTTGGAACTGGTAACACTTGACAGTAGATGCTCTCTAAAGTCCTTCAGGAGTGTGGTTTCTCAGTTTAGTGATGTGAAGAAGGCAGCATGTGAAGAGCTCGGTTTTGGTGTGTTGTTGAAGATTGCTGGGACGAGACTGCGCCACTCGATGGTGCGCGCTCTTGTGGATTTGGTAGACCCCGAAAAAGGAACCATTCAGCTGCATGGGAAGTTGATTGAAATTTTTGCGTCTGATGTTGCTCAGGTGATGGGGCTGCAGAATGGTGGGGAGGAGGTCATTCAGGCTGGTTCGTTCAAGGATCACCCTGAGTTGATGGATATAATCAGTCAGTTCTCTCGGTCTGATGGGAAGATCTATTCGAAGGAGTTGGTGAGGTATTTGGTGGATCCTCAAACGGTAGCCGACGACAAATTCAAGCGAGTGTTTGTTTGGTTCACACTGACCACTATCCTCAGGCCCACTGCCTCACTCTCCCTTCCGAAGAAGTGGTTGGTACTCCTCAAGGACACTAGCCGCATCTCATCCATGAACTGGGCTGCTTACTCTTTCCTATGGTTGATGAATGGAATCAAGGAGTTCAAGGGTGATAAGACCTACTGCAGCGGAAACCTCCTTTTTCTCCAGCTTTTCTACATGGATTGTGTCAAGAGCGGCCCAACCATCGTGGACAAGTCACTGCACCCGGTTGAAAGTTGGGGTGACCGAGAAGCAAATAAGCTGCTGGGATGGGTATACAATCAGGGTGGTTTGACGTCGGAGAGTGTTGATGTATCAAAGACCAAGGATCGTACATGGAGGGGGAGGGGACGTGTGGCCAGTAGCAAGGTCTCGGCAGACAAGTATGAGGAATTGGCTGGTAAAGTGGCTTCGCTGCAAGATGGCCTCGCTGAGGTATCGGGGAAGCTGCAAGATATGATGGACATGATGCAGACTTTAGTTTCCGCTCATAACAAGGCCAAGGAAGCTAATACAGAAGCAGCTGGGGTGTCACATGTGGAAAAGAAGAAGAGCGTGTCCATTGTGGATGTAGAATTTGACTCTTCTGTTTTGAGTCCTATAAGATCTGTAAAGAAGCCTTATGCAGTCATCAACAGTGTTGAGACAGTGAAATCTCCTTTGAACGATGTTGTGGAAGAGCATGACATGAACAAGGCGAAGACCAAAGTTGTTAGGAAAGAGAGAATTGGACCTTTTAGGGTGGACGCCGGCATGGATGAGGCTGACAAGGGTATCCTTAAATTTGTCTTTGAAAGTGAATTTCCACTTAAAGAAAACATTGTGGACACTGGTTCCGCTGGTAGTTGTACTCGGGATGGGGCTCAAAAATTCCAACCTAACGGATGGCTCGGGACATTTGCCGTGAACTGCTTCACTGACATATTACATCTAGAAAGGGGTGAGGACCATACTAGTGTGTTCTTCCCTACCTTCTTTGCGGAAAAGTTGTTTCCGGAAAATGCCAAGCCGAAAACTTCTTTGGGTCTGCGATCAAAAGACTTATGTGTGAGGAGATTTGATCATCTTATCCATACTTGCGAGAAGATTTTTATACCAGTCCATGATTCGGATCACTATTTCTTGTTCGTTGTGCGGTTGGATACTGAAGAAGCAGAATATTGGGATAGCATGCATGGCACAAATAGTAGGAGGACCAAGTGCAAGGCAATTCTGCAAGCACTGGACCATATTTACCGCAACAATGTATTTGTGACATCAAAGAAGAAGTTTACAGAATTCAGGTTGGTGGACCGGATGGAGGCCAAACAATCCGATGCTGATTGTGCTATTTTTGTGATCCGACACATGCAGCGTTATGGTGAGGAGTGGTGGCGTAATTTCAAATCAGAAGAGACTCGTATTGAACTGCTGCTTTATATGATAAAGAGCAAGTACAATAGCCTTTTGAGAATGGTTTTAAAAGAGACAGTGAAGAAGGAGGATACAAAGGAGGATAACTTAGAAAAGGCGGCAAGAATGAGGAAGGTTGAAGGAAAACAAAACACCAGGGCTCGGTCTAAGAAGGGTGTGAACAAAGATGTACGTCGCAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

875

Amino Acids

98.68

Weight (kDa)

9.26

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29141 FvH4_1g29142 FvH4_2g04882 FvH4_2g04883 FvH4_2g07612 FvH4_3g26550 FvH4_3g26551 FvH4_3g31781 FvH4_3g31810 FvH4_3g31812 FvH4_4g08580 FvH4_4g08581 FvH4_4g08649 FvH4_4g08910 FvH4_4g10124 FvH4_4g10125 FvH4_4g28760 FvH4_5g16042 FvH4_5g24070 FvH4_5g30680 FvH4_5g37871 FvH4_5g37872 FvH4_7g03531 FvH4_7g12891 FvH4_7g12892 FvH4_7g12893
rosa_chinensis RchiOBHm_Chr7g0204351
rosa_laevigata RLG00000001882 RLG00000002339 RLG00000004189 RLG00000009211 RLG00000009212 RLG00000013650 RLG00000019919 RLG00000019920 RLG00000019921 RLG00000020177 RLG00000020178 RLG00000023368 RLG00000028208 RLG00000029086 RLG00000029688 RLG00000032551 RLG00000034335 RLG00000034807
rosa_multiflora Rmu_sc0000308.1_g000017 Rmu_sc0000308.1_g000018 Rmu_sc0003422.1_g000004 Rmu_sc0003776.1_g000026 Rmu_sc0005399.1_g000010 Rmu_sc0005399.1_g000012 Rmu_sc0009924.1_g000004
rosa_roxburghii Rroxscaffold_1G00053720 Rroxscaffold_1G00053730 Rroxscaffold_1G00054020 Rroxscaffold_2G00133470 Rroxscaffold_2G00133480 Rroxscaffold_3G00229920 Rroxscaffold_3G00229930 Rroxscaffold_3G00229940 Rroxscaffold_3G00245890 Rroxscaffold_3G00245900 Rroxscaffold_4G00317860 Rroxscaffold_5G00346620 Rroxscaffold_6G00396640 Rroxscaffold_6G00396930 Rroxscaffold_6G00396940 Rroxscaffold_6G00401510 Rroxscaffold_7G00201920 Rroxscaffold_7G00201930 Rroxscaffold_7G00201940
rosa_rugosa Rorug01G0116700 Rorug01G0116700 Rorug04G0072900 Rorug04G0073000 Rorug04G0112000 Rorug07G0258800
rosa_samantha Rh1AG123800 Rh1AG297000 Rh1DG057400 Rh1DG057500 Rh1DG129000 Rh2BG298000 Rh2BG471200 Rh2BG471300 Rh2BG471400 Rh2BG621400 Rh3CG245500 Rh3CG245600 Rh4DG157200 Rh4DG157300 Rh4DG157400 Rh4DG157500 Rh4DG157600 Rh4DG157700 Rh5DG402200 Rh6AG050900 Rh6AG051000 Rh6AG088400 Rh6AG088500 Rh6AG088600 Rh6AG088700 Rh6AG139800 Rh6AG139900 Rh6AG140000 Rh6CG077200 Rh6CG077300 Rh6CG077400 Rh6CG077500 Rh7CG225300 Rh7CG225400 Rh7CG225500 Rh7CG225600 Rh7CG267500 Rh7CG267600 Rh7CG267800 Rh7CG267900 Rh7CG338700 Rh7CG338800 Rh7CG338900 Rh7CG430900 Rh7CG431000 Rh7CG431100 Rh7DG219700 Rh7DG219800 Rh7DG219900 Rh7DG220000 Rh7DG257600 Rh7DG271500 Rh7DG367700 Rh7DG408600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
AatII GACGTC 1 cut(s) 1325
AccB7I CCANNNNNTGG 2 cut(s) 718, 1101
AccBSI CCGCTC 2 cut(s) 1221, 1514
AccI GTMKAC 2 cut(s) 744, 1302
AccII CGCG 1 cut(s) 726
AccIII TCCGGA 1 cut(s) 2020
AclWI GGATC 8 cut(s) 155, 188, 876, 950, 963, 1362, 2145, 2359
AcoI YGGCCR 2 cut(s) 336, 1383
AcsI RAATTY 8 cut(s) 253, 789, 980, 1601, 1808, 1826, 1895, 2303
AcuI CTGAAG 4 cut(s) 407, 419, 598, 2193
AcyI GRCGYC 3 cut(s) 320, 1322, 1773
AfaI GTAC 7 cut(s) 496, 524, 1065, 1360, 1876, 2462, 2610
AfiI CCNNNNNNNGG 9 cut(s) 151, 417, 718, 755, 1101, 1459, 2134, 2326, 2580
AflIII ACRYGT 2 cut(s) 1378, 1561
AhlI ACTAGT 1 cut(s) 1979
AjiI CACGTC 1 cut(s) 1379
AjnI CCWGG 1 cut(s) 2573
AjuI GAANNNNNNNTTGG 4 cut(s) 1043, 1075, 2030, 2062
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 7 cut(s) 193, 384, 697, 1402, 1664, 2414, 2486
AlwI GGATC 8 cut(s) 155, 188, 876, 950, 963, 1362, 2145, 2359
Ama87I CYCGRG 3 cut(s) 506, 1878, 1916
Aor13HI TCCGGA 1 cut(s) 2020
AoxI GGCC 8 cut(s) 60, 336, 1025, 1222, 1383, 1450, 1524, 2328
ApoI RAATTY 8 cut(s) 253, 789, 980, 1601, 1808, 1826, 1895, 2303
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
Asp700I GAANNNNTTC 1 cut(s) 1937
AspLEI GCGC 3 cut(s) 711, 726, 728
AspS9I GGNCC 8 cut(s) 61, 1026, 1223, 1757, 1972, 2220, 2251, 2317
AsuC2I CCSGG 2 cut(s) 23, 1253
AsuHPI GGTGA 9 cut(s) 37, 353, 826, 863, 952, 1166, 1280, 1979, 2399
AsuII TTCGAA 1 cut(s) 929
AvaI CYCGRG 3 cut(s) 506, 1878, 1916
AvaII GGWCC 5 cut(s) 1757, 1972, 2220, 2251, 2317
AxyI CCTNAGG 1 cut(s) 1022
BaeGI GKGCMC 1 cut(s) 277
BaeI ACNNNNGTAYC 2 cut(s) 1783, 1816
BalI TGGCCA 1 cut(s) 1385
BamHI GGATCC 1 cut(s) 955
BanII GRGCYC 3 cut(s) 669, 1891, 2581
BarI GAAGNNNNNNTAC 2 cut(s) 1420, 1452
BbsI GAAGAC 2 cut(s) 471, 1733
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 1458
BceAI ACGGC 1 cut(s) 1913
BciT130I CCWGG 1 cut(s) 2575
BciVI GTATCC 3 cut(s) 1811, 2161, 2503
BclI TGATCA 1 cut(s) 2086
BcnI CCSGG 2 cut(s) 23, 1253
BcoDI GTCTC 7 cut(s) 193, 384, 697, 1402, 1664, 2414, 2486
BcuI ACTAGT 1 cut(s) 1979
BfaI CTAG 4 cut(s) 357, 1080, 1958, 1980
BfmI CTRYAG 3 cut(s) 172, 824, 1168
BfuI GTATCC 3 cut(s) 1811, 2161, 2503
BglII AGATCT 2 cut(s) 921, 1631
Bme1390I CCNGG 3 cut(s) 23, 1253, 2575
Bme18I GGWCC 5 cut(s) 1757, 1972, 2220, 2251, 2317
BmeT110I CYCGRG 3 cut(s) 506, 1878, 1916
BmgBI CACGTC 1 cut(s) 1379
BmgT120I GGNCC 8 cut(s) 61, 1026, 1223, 1757, 1972, 2220, 2251, 2317
BmiI GGNNCC 5 cut(s) 63, 261, 760, 957, 1861
BmrFI CCNGG 3 cut(s) 23, 1253, 2575
BmrI ACTGGG 1 cut(s) 1111
BmsI GCATC 5 cut(s) 544, 587, 1095, 1485, 2332
BmuI ACTGGG 1 cut(s) 1111
BpiI GAAGAC 2 cut(s) 471, 1733
BpmI CTGGAG 1 cut(s) 1175
Bpu10I CCTNAGC 2 cut(s) 810, 1458
Bpu14I TTCGAA 1 cut(s) 929
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 2 cut(s) 23, 1253
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 2136
BsaHI GRCGYC 3 cut(s) 320, 1322, 1773
BsaI GGTCTC 2 cut(s) 384, 1402
BsaJI CCNNGG 6 cut(s) 21, 78, 111, 1350, 1527, 2574
BsaWI WCCGGW 2 cut(s) 2020, 2319
BsaXI ACNNNNNCTCC 2 cut(s) 1363, 1393
Bsc4I CCNNNNNNNGG 9 cut(s) 151, 417, 718, 755, 1101, 1459, 2134, 2326, 2580
Bse118I RCCGGY 2 cut(s) 338, 1775
Bse1I ACTGG 7 cut(s) 270, 582, 1106, 1386, 1861, 2123, 2253
Bse21I CCTNAGG 1 cut(s) 1022
Bse8I GATNNNNATC 1 cut(s) 2136
BseAI TCCGGA 1 cut(s) 2020
BseBI CCWGG 1 cut(s) 2575
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 6 cut(s) 21, 78, 111, 1350, 1527, 2574
BseGI GGATG 7 cut(s) 1091, 1301, 1600, 1789, 1888, 1916, 2328
BseJI GATNNNNATC 1 cut(s) 2136
BseLI CCNNNNNNNGG 9 cut(s) 151, 417, 718, 755, 1101, 1459, 2134, 2326, 2580
BseMII CTCAG 5 cut(s) 644, 824, 867, 1036, 1449
BseNI ACTGG 7 cut(s) 270, 582, 1106, 1386, 1861, 2123, 2253
BsePI GCGCGC 1 cut(s) 724
BseRI GAGGAG 5 cut(s) 359, 854, 1058, 2092, 2405
BseSI GKGCMC 1 cut(s) 277
BseYI CCCAGC 3 cut(s) 695, 1291, 1550
BsgI GTGCAG 1 cut(s) 1232
Bsh1236I CGCG 1 cut(s) 726
BshFI GGCC 8 cut(s) 62, 338, 1027, 1224, 1385, 1452, 1526, 2330
BshVI ATCGAT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 3 cut(s) 506, 1878, 1916
BsiSI CCGG 6 cut(s) 23, 339, 1253, 1776, 2021, 2320
BslFI GGGAC 3 cut(s) 712, 1389, 1933
BslI CCNNNNNNNGG 9 cut(s) 151, 417, 718, 755, 1101, 1459, 2134, 2326, 2580
BsmAI GTCTC 7 cut(s) 193, 384, 697, 1402, 1664, 2414, 2486
BsmFI GGGAC 3 cut(s) 712, 1389, 1933
BsnI GGCC 8 cut(s) 62, 338, 1027, 1224, 1385, 1452, 1526, 2330
Bso31I GGTCTC 2 cut(s) 384, 1402
BsoBI CYCGRG 3 cut(s) 506, 1878, 1916
Bsp119I TTCGAA 1 cut(s) 929
Bsp1286I GDGCHC 4 cut(s) 277, 669, 1891, 2581
Bsp13I TCCGGA 1 cut(s) 2020
BspANI GGCC 8 cut(s) 62, 338, 1027, 1224, 1385, 1452, 1526, 2330
BspCNI CTCAG 5 cut(s) 643, 823, 868, 1035, 1450
BspDI ATCGAT 1 cut(s) 159
BspEI TCCGGA 1 cut(s) 2020
BspFNI CGCG 1 cut(s) 726
BspLI GGNNCC 5 cut(s) 63, 261, 760, 957, 1861
BspMAI CTGCAG 3 cut(s) 176, 828, 1172
BspPI GGATC 8 cut(s) 155, 188, 876, 950, 963, 1362, 2145, 2359
BspQI GCTCTTC 3 cut(s) 657, 1571, 1698
BspT104I TTCGAA 1 cut(s) 929
BspTNI GGTCTC 2 cut(s) 384, 1402
BsrBI CCGCTC 2 cut(s) 1221, 1514
BsrFI RCCGGY 2 cut(s) 338, 1775
BsrI ACTGG 7 cut(s) 270, 582, 1106, 1386, 1861, 2123, 2253
BssAI RCCGGY 2 cut(s) 338, 1775
BssECI CCNNGG 6 cut(s) 21, 78, 111, 1350, 1527, 2574
BssHII GCGCGC 1 cut(s) 724
BssNAI GTATAC 1 cut(s) 1303
BssNI GRCGYC 3 cut(s) 320, 1322, 1773
BssT1I CCWWGG 3 cut(s) 78, 1350, 1527
Bst1107I GTATAC 1 cut(s) 1303
Bst2UI CCWGG 1 cut(s) 2575
Bst4CI ACNGT 6 cut(s) 187, 593, 967, 1664, 1675, 2497
Bst6I CTCTTC 6 cut(s) 131, 657, 1571, 1615, 1698, 2412
BstACI GRCGYC 3 cut(s) 320, 1322, 1773
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 929
BstC8I GCNNGC 4 cut(s) 726, 1777, 2198, 2244
BstDEI CTNAG 7 cut(s) 630, 810, 876, 1022, 1458, 2527, 2586
BstEII GGTNACC 1 cut(s) 1268
BstENI CCTNNNNNAGG 1 cut(s) 1457
BstF5I GGATG 7 cut(s) 1091, 1301, 1600, 1789, 1888, 1916, 2328
BstFNI CGCG 1 cut(s) 726
BstHHI GCGC 3 cut(s) 711, 726, 728
BstMAI GTCTC 7 cut(s) 193, 384, 697, 1402, 1664, 2414, 2486
BstMWI GCNNNNNNNGC 7 cut(s) 116, 305, 317, 554, 1532, 2202, 2351
BstNI CCWGG 1 cut(s) 2575
BstNSI RCATGY 4 cut(s) 660, 1565, 2200, 2377
BstPI GGTNACC 1 cut(s) 1268
BstSCI CCNGG 3 cut(s) 21, 1251, 2573
BstSFI CTRYAG 3 cut(s) 172, 824, 1168
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 1 cut(s) 726
BstV2I GAAGAC 2 cut(s) 471, 1733
BstX2I RGATCY 3 cut(s) 921, 955, 1631
BstYI RGATCY 3 cut(s) 921, 955, 1631
BstZ17I GTATAC 1 cut(s) 1303
Bsu15I ATCGAT 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 1022
BsuI GTATCC 3 cut(s) 1811, 2161, 2503
BsuRI GGCC 8 cut(s) 62, 338, 1027, 1224, 1385, 1452, 1526, 2330
BsuTUI ATCGAT 1 cut(s) 159
BtrI CACGTC 1 cut(s) 1379
BtsCI GGATG 7 cut(s) 1091, 1301, 1600, 1789, 1888, 1916, 2328
BtsI GCAGTG 2 cut(s) 1029, 1244
Cac8I GCNNGC 4 cut(s) 726, 1777, 2198, 2244
CfoI GCGC 3 cut(s) 711, 726, 728
Cfr10I RCCGGY 2 cut(s) 338, 1775
Cfr13I GGNCC 8 cut(s) 61, 1026, 1223, 1757, 1972, 2220, 2251, 2317
ClaI ATCGAT 1 cut(s) 159
CseI GACGC 4 cut(s) 125, 309, 786, 1781
Csp6I GTAC 7 cut(s) 495, 523, 1064, 1359, 1875, 2461, 2609
CspCI CAANNNNNGTGG 4 cut(s) 1215, 1250, 1294, 1329
CviQI GTAC 7 cut(s) 495, 523, 1064, 1359, 1875, 2461, 2609
DdeI CTNAG 7 cut(s) 630, 810, 876, 1022, 1458, 2527, 2586
DraI TTTAAA 2 cut(s) 432, 2487
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 2 cut(s) 336, 1383
Eam1104I CTCTTC 6 cut(s) 131, 657, 1571, 1615, 1698, 2412
EarI CTCTTC 6 cut(s) 131, 657, 1571, 1615, 1698, 2412
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 3 cut(s) 78, 1350, 1527
Eco24I GRGCYC 3 cut(s) 669, 1891, 2581
Eco31I GGTCTC 2 cut(s) 384, 1402
Eco47I GGWCC 5 cut(s) 1757, 1972, 2220, 2251, 2317
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 4 cut(s) 407, 419, 598, 2193
Eco81I CCTNAGG 1 cut(s) 1022
Eco88I CYCGRG 3 cut(s) 506, 1878, 1916
Eco91I GGTNACC 1 cut(s) 1268
EcoICRI GAGCTC 1 cut(s) 667
EcoNI CCTNNNNNAGG 1 cut(s) 1457
EcoO109I RGGNCCY 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 1268
EcoRI GAATTC 1 cut(s) 2303
EcoRII CCWGG 1 cut(s) 2573
EcoT14I CCWWGG 3 cut(s) 78, 1350, 1527
EcoT22I ATGCAT 1 cut(s) 2202
EcoT38I GRGCYC 3 cut(s) 669, 1891, 2581
ErhI CCWWGG 3 cut(s) 78, 1350, 1527
FaqI GGGAC 3 cut(s) 712, 1389, 1933
FbaI TGATCA 1 cut(s) 2086
FblI GTMKAC 2 cut(s) 744, 1302
FokI GGATG 7 cut(s) 1078, 1308, 1607, 1796, 1895, 1923, 2335
FriOI GRGCYC 3 cut(s) 669, 1891, 2581
FspBI CTAG 4 cut(s) 357, 1080, 1958, 1980
GlaI GCGC 3 cut(s) 710, 725, 727
GsaI CCCAGC 3 cut(s) 699, 1295, 1554
GsuI CTGGAG 1 cut(s) 1175
HaeIII GGCC 8 cut(s) 62, 338, 1027, 1224, 1385, 1452, 1526, 2330
HapII CCGG 6 cut(s) 23, 339, 1253, 1776, 2021, 2320
HgaI GACGC 4 cut(s) 125, 309, 786, 1781
HhaI GCGC 3 cut(s) 711, 726, 728
Hin1I GRCGYC 3 cut(s) 320, 1322, 1773
Hin6I GCGC 3 cut(s) 709, 724, 726
HinP1I GCGC 3 cut(s) 709, 724, 726
HinfI GANTC 8 cut(s) 266, 476, 566, 1137, 1607, 1621, 2132, 2422
HpaII CCGG 6 cut(s) 23, 339, 1253, 1776, 2021, 2320
HphI GGTGA 9 cut(s) 37, 353, 826, 863, 952, 1166, 1280, 1979, 2399
Hpy99I CGWCG 6 cut(s) 70, 316, 325, 977, 1327, 2616
HpyCH4III ACNGT 6 cut(s) 187, 593, 967, 1664, 1675, 2497
HpyCH4IV ACGT 4 cut(s) 497, 1322, 1378, 2611
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 305, 317, 554, 1532, 2202, 2351
HpyF3I CTNAG 7 cut(s) 630, 810, 876, 1022, 1458, 2527, 2586
HpySE526I ACGT 4 cut(s) 497, 1322, 1378, 2611
Hsp92I GRCGYC 3 cut(s) 320, 1322, 1773
HspAI GCGC 3 cut(s) 709, 724, 726
Kpn2I TCCGGA 1 cut(s) 2020
KroI GCCGGC 1 cut(s) 1775
KroNI GCCGGC 1 cut(s) 1777
Ksp22I TGATCA 1 cut(s) 2086
LguI GCTCTTC 3 cut(s) 657, 1571, 1698
LmnI GCTCC 3 cut(s) 182, 214, 265
LweI GCATC 5 cut(s) 544, 587, 1095, 1485, 2332
MaeI CTAG 4 cut(s) 357, 1080, 1958, 1980
MaeII ACGT 4 cut(s) 497, 1322, 1378, 2611
MaeIII GTNAC 5 cut(s) 580, 1242, 1268, 1557, 2278
MbiI CCGCTC 2 cut(s) 1221, 1514
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 3 cut(s) 921, 955, 1631
MhlI GDGCHC 4 cut(s) 277, 669, 1891, 2581
MlsI TGGCCA 1 cut(s) 1385
MluNI TGGCCA 1 cut(s) 1385
MlyI GAGTC 5 cut(s) 485, 575, 1601, 1630, 2416
MmeI TCCRAC 6 cut(s) 204, 435, 1305, 1924, 2145, 2392
Mox20I TGGCCA 1 cut(s) 1385
Mph1103I ATGCAT 1 cut(s) 2202
MroI TCCGGA 1 cut(s) 2020
MroNI GCCGGC 1 cut(s) 1775
MroXI GAANNNNTTC 1 cut(s) 1937
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 4 cut(s) 431, 1806, 1836, 2486
MslI CAYNNNNRTG 2 cut(s) 487, 1980
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 4 cut(s) 769, 1173, 1550, 1865
MspI CCGG 6 cut(s) 23, 339, 1253, 1776, 2021, 2320
MspR9I CCNGG 3 cut(s) 23, 1253, 2575
MunI CAATTG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 2575
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 7 cut(s) 116, 305, 317, 554, 1532, 2202, 2351
NaeI GCCGGC 1 cut(s) 1777
NciI CCSGG 2 cut(s) 23, 1253
NgoMIV GCCGGC 1 cut(s) 1775
NlaIV GGNNCC 5 cut(s) 63, 261, 760, 957, 1861
NmeAIII GCCGAG 1 cut(s) 1379
NmuCI GTSAC 4 cut(s) 1242, 1268, 1557, 2278
NsiI ATGCAT 1 cut(s) 2202
NspI RCATGY 4 cut(s) 660, 1565, 2200, 2377
NspV TTCGAA 1 cut(s) 929
PaeI GCATGC 1 cut(s) 2200
PaeR7I CTCGAG 1 cut(s) 506
PauI GCGCGC 1 cut(s) 724
PciI ACATGT 1 cut(s) 1561
PciSI GCTCTTC 3 cut(s) 657, 1571, 1698
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PdiI GCCGGC 1 cut(s) 1777
PdmI GAANNNNTTC 1 cut(s) 1937
PfeI GAWTC 3 cut(s) 266, 1137, 2132
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 718, 1101
PleI GAGTC 5 cut(s) 484, 574, 1601, 1629, 2416
PpsI GAGTC 5 cut(s) 484, 574, 1601, 1629, 2416
PscI ACATGT 1 cut(s) 1561
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 2573
PspEI GGTNACC 1 cut(s) 1268
PspFI CCCAGC 3 cut(s) 695, 1291, 1550
PspGI CCWGG 1 cut(s) 2573
PspN4I GGNNCC 5 cut(s) 63, 261, 760, 957, 1861
PspPI GGNCC 8 cut(s) 61, 1026, 1223, 1757, 1972, 2220, 2251, 2317
PstI CTGCAG 3 cut(s) 176, 828, 1172
PsuI RGATCY 3 cut(s) 921, 955, 1631
PsyI GACNNNGTC 1 cut(s) 185
PteI GCGCGC 1 cut(s) 724
PvuII CAGCTG 2 cut(s) 769, 1550
RsaI GTAC 7 cut(s) 496, 524, 1065, 1360, 1876, 2462, 2610
RsaNI GTAC 7 cut(s) 495, 523, 1064, 1359, 1875, 2461, 2609
RseI CAYNNNNRTG 2 cut(s) 487, 1980
SacI GAGCTC 1 cut(s) 669
SapI GCTCTTC 3 cut(s) 657, 1571, 1698
SaqAI TTAA 4 cut(s) 431, 1806, 1836, 2486
Sau96I GGNCC 8 cut(s) 61, 1026, 1223, 1757, 1972, 2220, 2251, 2317
SchI GAGTC 5 cut(s) 485, 575, 1601, 1630, 2416
ScrFI CCNGG 3 cut(s) 23, 1253, 2575
SduI GDGCHC 4 cut(s) 277, 669, 1891, 2581
SfaNI GCATC 5 cut(s) 544, 587, 1095, 1485, 2332
SfcI CTRYAG 3 cut(s) 172, 824, 1168
Sfr274I CTCGAG 1 cut(s) 506
SfuI TTCGAA 1 cut(s) 929
SinI GGWCC 5 cut(s) 1757, 1972, 2220, 2251, 2317
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 487, 1980
SmlI CTYRAG 2 cut(s) 506, 1070
SmoI CTYRAG 2 cut(s) 506, 1070
SpeI ACTAGT 1 cut(s) 1979
SphI GCATGC 1 cut(s) 2200
SspI AATATT 1 cut(s) 2186
SspMI CTAG 4 cut(s) 357, 1080, 1958, 1980
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 3 cut(s) 21, 1251, 2573
StyI CCWWGG 3 cut(s) 78, 1350, 1527
TaaI ACNGT 6 cut(s) 187, 593, 967, 1664, 1675, 2497
TaiI ACGT 4 cut(s) 500, 1325, 1381, 2614
TaqI TCGA 5 cut(s) 159, 323, 507, 716, 929
TaqII GACCGA 3 cut(s) 897, 1287, 2571
TatI WGTACW 3 cut(s) 522, 1874, 2460
TauI GCSGC 5 cut(s) 7, 52, 1086, 1224, 2540
TfiI GAWTC 3 cut(s) 266, 1137, 2132
Tru1I TTAA 4 cut(s) 431, 1806, 1836, 2486
Tru9I TTAA 4 cut(s) 431, 1806, 1836, 2486
TseFI GTSAC 4 cut(s) 1242, 1268, 1557, 2278
Tsp45I GTSAC 4 cut(s) 1242, 1268, 1557, 2278
TspDTI ATGAA 4 cut(s) 318, 1112, 1145, 1730
TspGWI ACGGA 2 cut(s) 329, 1923
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 718, 1101
VpaK11BI GGWCC 5 cut(s) 1757, 1972, 2220, 2251, 2317
XagI CCTNNNNNAGG 1 cut(s) 1457
XapI RAATTY 8 cut(s) 253, 789, 980, 1601, 1808, 1826, 1895, 2303
XbaI TCTAGA 1 cut(s) 1957
XceI RCATGY 4 cut(s) 660, 1565, 2200, 2377
XhoI CTCGAG 1 cut(s) 506
XmiI GTMKAC 2 cut(s) 744, 1302
XmnI GAANNNNTTC 1 cut(s) 1937
XspI CTAG 4 cut(s) 357, 1080, 1958, 1980
ZraI GACGTC 1 cut(s) 1323
Zsp2I ATGCAT 1 cut(s) 2202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.