PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 828 |
| Acc36I | ACCTGC | 1 cut(s) | 828 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 442 |
| AclWI | GGATC | 2 cut(s) | 580, 674 |
| AcoI | YGGCCR | 1 cut(s) | 689 |
| AcsI | RAATTY | 2 cut(s) | 157, 644 |
| AcuI | CTGAAG | 1 cut(s) | 147 |
| AdeI | CACNNNGTG | 1 cut(s) | 442 |
| AfaI | GTAC | 1 cut(s) | 609 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 442, 752 |
| AflIII | ACRYGT | 1 cut(s) | 330 |
| AgsI | TTSAA | 5 cut(s) | 61, 212, 451, 629, 649 |
| AhlI | ACTAGT | 1 cut(s) | 167 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 166, 198 |
| AluBI | AGCT | 2 cut(s) | 125, 797 |
| AluI | AGCT | 2 cut(s) | 125, 797 |
| Alw21I | GWGCWC | 2 cut(s) | 102, 799 |
| Alw26I | GTCTC | 2 cut(s) | 131, 321 |
| AlwI | GGATC | 2 cut(s) | 580, 674 |
| AoxI | GGCC | 1 cut(s) | 689 |
| ApeKI | GCWGC | 1 cut(s) | 740 |
| ApoI | RAATTY | 2 cut(s) | 157, 644 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 644 |
| AspS9I | GGNCC | 1 cut(s) | 756 |
| AsuII | TTCGAA | 1 cut(s) | 359 |
| AvaII | GGWCC | 1 cut(s) | 756 |
| BanII | GRGCYC | 1 cut(s) | 799 |
| Bbv12I | GWGCWC | 2 cut(s) | 102, 799 |
| BbvI | GCAGC | 1 cut(s) | 752 |
| BccI | CCATC | 2 cut(s) | 198, 686 |
| BceAI | ACGGC | 1 cut(s) | 735 |
| BclI | TGATCA | 1 cut(s) | 549 |
| BcoDI | GTCTC | 2 cut(s) | 131, 321 |
| BcuI | ACTAGT | 1 cut(s) | 167 |
| BfaI | CTAG | 2 cut(s) | 168, 792 |
| BfuAI | ACCTGC | 1 cut(s) | 828 |
| BisI | GCNGC | 1 cut(s) | 741 |
| BlsI | GCNGC | 1 cut(s) | 742 |
| Bme18I | GGWCC | 1 cut(s) | 756 |
| BmgT120I | GGNCC | 1 cut(s) | 756 |
| BmiI | GGNNCC | 1 cut(s) | 757 |
| BmsI | GCATC | 3 cut(s) | 4, 595, 827 |
| Bpu14I | TTCGAA | 1 cut(s) | 359 |
| Bsa29I | ATCGAT | 1 cut(s) | 669 |
| BsaI | GGTCTC | 1 cut(s) | 131 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 442, 752 |
| Bse118I | RCCGGY | 1 cut(s) | 499 |
| Bse1I | ACTGG | 2 cut(s) | 426, 487 |
| BseCI | ATCGAT | 1 cut(s) | 669 |
| BseGI | GGATG | 2 cut(s) | 209, 774 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 442, 752 |
| BseMII | CTCAG | 3 cut(s) | 390, 567, 612 |
| BseNI | ACTGG | 2 cut(s) | 426, 487 |
| BseRI | GAGGAG | 1 cut(s) | 95 |
| BseXI | GCAGC | 1 cut(s) | 752 |
| BshFI | GGCC | 1 cut(s) | 691 |
| BshVI | ATCGAT | 1 cut(s) | 669 |
| BsiHKAI | GWGCWC | 2 cut(s) | 102, 799 |
| BsiSI | CCGG | 1 cut(s) | 500 |
| BslFI | GGGAC | 1 cut(s) | 769 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 442, 752 |
| BsmAI | GTCTC | 2 cut(s) | 131, 321 |
| BsmFI | GGGAC | 1 cut(s) | 769 |
| BsmI | GAATGC | 1 cut(s) | 512 |
| BsnI | GGCC | 1 cut(s) | 691 |
| Bso31I | GGTCTC | 1 cut(s) | 131 |
| Bsp119I | TTCGAA | 1 cut(s) | 359 |
| Bsp1286I | GDGCHC | 2 cut(s) | 102, 799 |
| Bsp143I | GATC | 3 cut(s) | 549, 572, 666 |
| BspANI | GGCC | 1 cut(s) | 691 |
| BspCNI | CTCAG | 3 cut(s) | 391, 568, 611 |
| BspDI | ATCGAT | 1 cut(s) | 669 |
| BspLI | GGNNCC | 1 cut(s) | 757 |
| BspMI | ACCTGC | 1 cut(s) | 828 |
| BspPI | GGATC | 2 cut(s) | 580, 674 |
| BspT104I | TTCGAA | 1 cut(s) | 359 |
| BspTNI | GGTCTC | 1 cut(s) | 131 |
| BsrFI | RCCGGY | 1 cut(s) | 499 |
| BsrI | ACTGG | 2 cut(s) | 426, 487 |
| BssAI | RCCGGY | 1 cut(s) | 499 |
| BssMI | GATC | 3 cut(s) | 549, 572, 666 |
| Bst4CI | ACNGT | 2 cut(s) | 536, 760 |
| Bst6I | CTCTTC | 2 cut(s) | 123, 597 |
| BstBI | TTCGAA | 1 cut(s) | 359 |
| BstC8I | GCNNGC | 1 cut(s) | 501 |
| BstDEI | CTNAG | 5 cut(s) | 251, 399, 576, 598, 714 |
| BstF5I | GGATG | 2 cut(s) | 209, 774 |
| BstKTI | GATC | 3 cut(s) | 552, 575, 669 |
| BstMAI | GTCTC | 2 cut(s) | 131, 321 |
| BstMBI | GATC | 3 cut(s) | 549, 572, 666 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 264 |
| BstNSI | RCATGY | 2 cut(s) | 334, 562 |
| BstV1I | GCAGC | 1 cut(s) | 752 |
| BstX2I | RGATCY | 1 cut(s) | 572 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 631 |
| BstYI | RGATCY | 1 cut(s) | 572 |
| Bsu15I | ATCGAT | 1 cut(s) | 669 |
| BsuRI | GGCC | 1 cut(s) | 691 |
| BsuTUI | ATCGAT | 1 cut(s) | 669 |
| BtsCI | GGATG | 2 cut(s) | 209, 774 |
| BtsI | GCAGTG | 1 cut(s) | 569 |
| BtsIMutI | CAGTG | 2 cut(s) | 419, 569 |
| BveI | ACCTGC | 1 cut(s) | 828 |
| Cac8I | GCNNGC | 1 cut(s) | 501 |
| Cfr10I | RCCGGY | 1 cut(s) | 499 |
| Cfr13I | GGNCC | 1 cut(s) | 756 |
| ClaI | ATCGAT | 1 cut(s) | 669 |
| CseI | GACGC | 1 cut(s) | 264 |
| Csp6I | GTAC | 1 cut(s) | 608 |
| CviAII | CATG | 4 cut(s) | 331, 440, 559, 613 |
| CviJI | RGCY | 7 cut(s) | 86, 125, 499, 503, 677, 691, 797 |
| CviKI_1 | RGCY | 7 cut(s) | 86, 125, 499, 503, 677, 691, 797 |
| CviQI | GTAC | 1 cut(s) | 608 |
| DdeI | CTNAG | 5 cut(s) | 251, 399, 576, 598, 714 |
| DpnI | GATC | 3 cut(s) | 551, 574, 668 |
| DpnII | GATC | 3 cut(s) | 549, 572, 666 |
| DraIII | CACNNNGTG | 1 cut(s) | 442 |
| EaeI | YGGCCR | 1 cut(s) | 689 |
| Eam1104I | CTCTTC | 2 cut(s) | 123, 597 |
| EarI | CTCTTC | 2 cut(s) | 123, 597 |
| Ecl136II | GAGCTC | 1 cut(s) | 797 |
| Eco24I | GRGCYC | 1 cut(s) | 799 |
| Eco31I | GGTCTC | 1 cut(s) | 131 |
| Eco32I | GATATC | 1 cut(s) | 74 |
| Eco47I | GGWCC | 1 cut(s) | 756 |
| Eco53kI | GAGCTC | 1 cut(s) | 797 |
| Eco57I | CTGAAG | 1 cut(s) | 147 |
| EcoICRI | GAGCTC | 1 cut(s) | 797 |
| EcoRV | GATATC | 1 cut(s) | 74 |
| EcoT38I | GRGCYC | 1 cut(s) | 799 |
| FaeI | CATG | 4 cut(s) | 334, 443, 562, 616 |
| FaqI | GGGAC | 1 cut(s) | 769 |
| FatI | CATG | 4 cut(s) | 330, 439, 558, 612 |
| FbaI | TGATCA | 1 cut(s) | 549 |
| Fnu4HI | GCNGC | 1 cut(s) | 741 |
| FokI | GGATG | 2 cut(s) | 216, 761 |
| FriOI | GRGCYC | 1 cut(s) | 799 |
| Fsp4HI | GCNGC | 1 cut(s) | 741 |
| FspBI | CTAG | 2 cut(s) | 168, 792 |
| GluI | GCNGC | 1 cut(s) | 741 |
| HaeIII | GGCC | 1 cut(s) | 691 |
| HapII | CCGG | 1 cut(s) | 500 |
| HgaI | GACGC | 1 cut(s) | 264 |
| Hin1II | CATG | 4 cut(s) | 334, 443, 562, 616 |
| HinfI | GANTC | 1 cut(s) | 341 |
| HpaII | CCGG | 1 cut(s) | 500 |
| Hpy188I | TCNGA | 5 cut(s) | 303, 532, 577, 601, 779 |
| Hpy188III | TCNNGA | 2 cut(s) | 77, 457 |
| Hpy99I | CGWCG | 1 cut(s) | 834 |
| HpyAV | CCTTC | 2 cut(s) | 127, 355 |
| HpyCH4III | ACNGT | 2 cut(s) | 536, 760 |
| HpyCH4V | TGCA | 2 cut(s) | 415, 562 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 264 |
| HpyF3I | CTNAG | 5 cut(s) | 251, 399, 576, 598, 714 |
| Hsp92II | CATG | 4 cut(s) | 334, 443, 562, 616 |
| KroI | GCCGGC | 1 cut(s) | 499 |
| KroNI | GCCGGC | 1 cut(s) | 501 |
| Ksp22I | TGATCA | 1 cut(s) | 549 |
| Kzo9I | GATC | 3 cut(s) | 549, 572, 666 |
| LmnI | GCTCC | 1 cut(s) | 105 |
| LpnPI | CCDG | 7 cut(s) | 116, 407, 411, 500, 513, 643, 823 |
| Lsp1109I | GCAGC | 1 cut(s) | 752 |
| LweI | GCATC | 3 cut(s) | 4, 595, 827 |
| MaeI | CTAG | 2 cut(s) | 168, 792 |
| MaeIII | GTNAC | 6 cut(s) | 35, 53, 186, 236, 278, 314 |
| MalI | GATC | 3 cut(s) | 551, 574, 668 |
| MboI | GATC | 3 cut(s) | 549, 572, 666 |
| MflI | RGATCY | 1 cut(s) | 572 |
| MhlI | GDGCHC | 2 cut(s) | 102, 799 |
| MluCI | AATT | 4 cut(s) | 157, 226, 629, 644 |
| MmeI | TCCRAC | 1 cut(s) | 802 |
| MnlI | CCTC | 6 cut(s) | 73, 76, 514, 598, 795, 801 |
| MroNI | GCCGGC | 1 cut(s) | 499 |
| MroXI | GAANNNNTTC | 1 cut(s) | 644 |
| MseI | TTAA | 3 cut(s) | 192, 594, 620 |
| MslI | CAYNNNNRTG | 1 cut(s) | 700 |
| MspA1I | CMGCKG | 1 cut(s) | 125 |
| MspI | CCGG | 1 cut(s) | 500 |
| Mva1269I | GAATGC | 1 cut(s) | 512 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 264 |
| NaeI | GCCGGC | 1 cut(s) | 501 |
| NdeII | GATC | 3 cut(s) | 549, 572, 666 |
| NgoMIV | GCCGGC | 1 cut(s) | 499 |
| NlaIII | CATG | 4 cut(s) | 334, 443, 562, 616 |
| NlaIV | GGNNCC | 1 cut(s) | 757 |
| NmeAIII | GCCGAG | 1 cut(s) | 667 |
| NmuCI | GTSAC | 2 cut(s) | 186, 236 |
| NspI | RCATGY | 2 cut(s) | 334, 562 |
| NspV | TTCGAA | 1 cut(s) | 359 |
| PaqCI | CACCTGC | 1 cut(s) | 828 |
| PciI | ACATGT | 1 cut(s) | 330 |
| PctI | GAATGC | 1 cut(s) | 512 |
| PdiI | GCCGGC | 1 cut(s) | 501 |
| PdmI | GAANNNNTTC | 1 cut(s) | 644 |
| PfeI | GAWTC | 1 cut(s) | 341 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 442 |
| PkrI | GCNGC | 1 cut(s) | 742 |
| PscI | ACATGT | 1 cut(s) | 330 |
| Psp124BI | GAGCTC | 1 cut(s) | 799 |
| PspN4I | GGNNCC | 1 cut(s) | 757 |
| PspPI | GGNCC | 1 cut(s) | 756 |
| PsuI | RGATCY | 1 cut(s) | 572 |
| PvuII | CAGCTG | 1 cut(s) | 125 |
| RsaI | GTAC | 1 cut(s) | 609 |
| RsaNI | GTAC | 1 cut(s) | 608 |
| RseI | CAYNNNNRTG | 1 cut(s) | 700 |
| SacI | GAGCTC | 1 cut(s) | 799 |
| SaqAI | TTAA | 3 cut(s) | 192, 594, 620 |
| SatI | GCNGC | 1 cut(s) | 741 |
| Sau3AI | GATC | 3 cut(s) | 549, 572, 666 |
| Sau96I | GGNCC | 1 cut(s) | 756 |
| SduI | GDGCHC | 2 cut(s) | 102, 799 |
| SetI | ASST | 8 cut(s) | 127, 199, 400, 609, 715, 787, 799, 842 |
| SfaNI | GCATC | 3 cut(s) | 4, 595, 827 |
| SfuI | TTCGAA | 1 cut(s) | 359 |
| SinI | GGWCC | 1 cut(s) | 756 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 700 |
| SpeI | ACTAGT | 1 cut(s) | 167 |
| Sse9I | AATT | 4 cut(s) | 157, 226, 629, 644 |
| SspMI | CTAG | 2 cut(s) | 168, 792 |
| SstI | GAGCTC | 1 cut(s) | 799 |
| TaaI | ACNGT | 2 cut(s) | 536, 760 |
| TaqI | TCGA | 5 cut(s) | 76, 172, 359, 495, 669 |
| TasI | AATT | 4 cut(s) | 157, 226, 629, 644 |
| TfiI | GAWTC | 1 cut(s) | 341 |
| Tru1I | TTAA | 3 cut(s) | 192, 594, 620 |
| Tru9I | TTAA | 3 cut(s) | 192, 594, 620 |
| TscAI | CASTG | 2 cut(s) | 426, 569 |
| TseFI | GTSAC | 2 cut(s) | 186, 236 |
| TseI | GCWGC | 1 cut(s) | 740 |
| Tsp45I | GTSAC | 2 cut(s) | 186, 236 |
| TspDTI | ATGAA | 3 cut(s) | 17, 323, 687 |
| TspGWI | ACGGA | 2 cut(s) | 216, 818 |
| TspRI | CASTG | 2 cut(s) | 426, 569 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 442 |
| VpaK11BI | GGWCC | 1 cut(s) | 756 |
| XapI | RAATTY | 2 cut(s) | 157, 644 |
| XceI | RCATGY | 2 cut(s) | 334, 562 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 811 |
| XmnI | GAANNNNTTC | 1 cut(s) | 644 |
| XspI | CTAG | 2 cut(s) | 168, 792 |