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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 170 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 121 |
| AdeI | CACNNNGTG | 1 cut(s) | 121 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 121 |
| AgsI | TTSAA | 1 cut(s) | 40 |
| Alw26I | GTCTC | 1 cut(s) | 2 |
| BbsI | GAAGAC | 2 cut(s) | 155, 166 |
| BcoDI | GTCTC | 1 cut(s) | 2 |
| BfaI | CTAG | 2 cut(s) | 84, 101 |
| BmrI | ACTGGG | 1 cut(s) | 72 |
| BmuI | ACTGGG | 1 cut(s) | 72 |
| BpiI | GAAGAC | 2 cut(s) | 155, 166 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 9, 39 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 121 |
| Bse1I | ACTGG | 1 cut(s) | 78 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BseNI | ACTGG | 1 cut(s) | 78 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BsmAI | GTCTC | 1 cut(s) | 2 |
| BsrI | ACTGG | 1 cut(s) | 78 |
| Bst4CI | ACNGT | 1 cut(s) | 133 |
| BstMAI | GTCTC | 1 cut(s) | 2 |
| BstV2I | GAAGAC | 2 cut(s) | 155, 166 |
| BtsIMutI | CAGTG | 2 cut(s) | 85, 138 |
| CviAII | CATG | 1 cut(s) | 170 |
| DraIII | CACNNNGTG | 1 cut(s) | 121 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 170 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 170 |
| FaeI | CATG | 1 cut(s) | 173 |
| FaiI | YATR | 4 cut(s) | 29, 74, 146, 171 |
| FatI | CATG | 1 cut(s) | 169 |
| FspBI | CTAG | 2 cut(s) | 84, 101 |
| Hin1II | CATG | 1 cut(s) | 173 |
| HinfI | GANTC | 1 cut(s) | 20 |
| Hpy166II | GTNNAC | 1 cut(s) | 129 |
| Hpy8I | GTNNAC | 1 cut(s) | 129 |
| HpyAV | CCTTC | 1 cut(s) | 34 |
| HpyCH4III | ACNGT | 1 cut(s) | 133 |
| HpyCH4V | TGCA | 1 cut(s) | 93 |
| Hsp92II | CATG | 1 cut(s) | 173 |
| LpnPI | CCDG | 2 cut(s) | 18, 91 |
| MaeI | CTAG | 2 cut(s) | 84, 101 |
| MboII | GAAGA | 3 cut(s) | 148, 160, 166 |
| MseI | TTAA | 1 cut(s) | 63 |
| NlaIII | CATG | 1 cut(s) | 173 |
| PfeI | GAWTC | 1 cut(s) | 20 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 121 |
| SaqAI | TTAA | 1 cut(s) | 63 |
| SetI | ASST | 1 cut(s) | 120 |
| SgeI | CNNG | 7 cut(s) | 17, 24, 60, 90, 96, 113, 131 |
| SspMI | CTAG | 2 cut(s) | 84, 101 |
| TaaI | ACNGT | 1 cut(s) | 133 |
| TaqI | TCGA | 1 cut(s) | 177 |
| TfiI | GAWTC | 1 cut(s) | 20 |
| Tru1I | TTAA | 1 cut(s) | 63 |
| Tru9I | TTAA | 1 cut(s) | 63 |
| TscAI | CASTG | 2 cut(s) | 85, 138 |
| TspDTI | ATGAA | 1 cut(s) | 161 |
| TspRI | CASTG | 2 cut(s) | 85, 138 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 121 |
| XspI | CTAG | 2 cut(s) | 84, 101 |