Rmu_sc0009924.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009924.1
OG0000269
Physical Location & Seq
Forward (+)
18001 .. 20348
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009924.1_g000004.1.cds

Sequence Viewer

Length: 1563 bp
atgttgcgtgtccttgtggactccgtagaccccaatgcatgtactataactatgcacggaaagaagattgaaatggatgcaagtgactttgaaaatgtgatggggttgaagaatgctgggtccgatgtcgactttaaaggttccactaatgatcaccctgagttgatggcgataataaactccttgtgtgcgaaggataagaagatcagtttaagggacgtgcaaaactacctgaaggatacagaagaagttgacaacaagttcaagcacctgtttgtgttgttcacaatgagcaccatccttagcccatctgcctcactgacaataccaaagaagtggctgctggccctgaatgacactcgcctgattagctctttaaactgggctgattactcattcaaatgtttgatggaggccatcgtcagtttcaaaaaagactcccggtcctattgtagcggttgtatcctgtttctgcaactgttctattttgattgtgtttcacatgggaagaccattgtggacaagtccttgtacccagttgaagcttggggggaccatgaaacaggcaaactgctcaaatgggtgagcaagcaaggtggtttgctggatgaaaatgttcttgtggcaaaggttggcgatcgtgttggtagtggagatcagaccaaagcatatggggtgattaagaaagaaatgtctgacttggctgctaaagtgaatgggttggaaaataatatgtgcaatctgagagatgatatgatgggaaagatggacaaagtcttgagtatgctgaacactctgactgcacaaaatgtgaatgtcaatcagaaccttcaaactgatggcttagcagaaaaagtacatggggaagacttgcaaccaaaaaagaagacctccttgatttgcctatctgactcaacatctgtagtcagcccactaaaaagtatgaagaaagttgatgccaaccccacgaccaagagtaacatagagaagattggtaacttcaaagtcaatggacatcgggaggaggctgacaaaggagtgctcctatttgtttatgatgtgtcagctgaagagaaggagaccattgtggatagtgaaaatttcttcactagtcgaaaagcagtttggtcacttaaacctaacggatgggttgaagatgatattatcaatttgtttagtgactatctatttctaggacgcttcccaagcaatagatgttgttacttcacaacatacttttgtcagaagatgaaaagttacaatgatagagacatgtgtggagattcagcaagggcagtttcgaagggtttgggtgtaaagagatttgagaatggcatacatgagtgtgacaagttgcaaacactggacattatctaccaccatgtggagttcaaagtcaagaatgttgtgaagaagtttgctgacttccagttattcaagccggctgaatgccctaaacaagagggctgttctgactgtagggtgtatgtgatcaaacacatgcagtgttatggatctgagtggtggcatcaggtatgtttgtcatttctgcatgaggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

520

Amino Acids

58.79

Weight (kDa)

6.68

Isoelectric Point (pI)

24.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000269)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29141 FvH4_1g29142 FvH4_2g04882 FvH4_2g04883 FvH4_2g07612 FvH4_3g26550 FvH4_3g26551 FvH4_3g31781 FvH4_3g31810 FvH4_3g31812 FvH4_4g08580 FvH4_4g08581 FvH4_4g08649 FvH4_4g08910 FvH4_4g10124 FvH4_4g10125 FvH4_4g28760 FvH4_5g16042 FvH4_5g24070 FvH4_5g30680 FvH4_5g37871 FvH4_5g37872 FvH4_7g03531 FvH4_7g12891 FvH4_7g12892 FvH4_7g12893
rosa_chinensis RchiOBHm_Chr7g0204351
rosa_laevigata RLG00000001882 RLG00000002339 RLG00000004189 RLG00000009211 RLG00000009212 RLG00000013650 RLG00000019919 RLG00000019920 RLG00000019921 RLG00000020177 RLG00000020178 RLG00000023368 RLG00000028208 RLG00000029086 RLG00000029688 RLG00000032551 RLG00000034335 RLG00000034807
rosa_multiflora Rmu_sc0000308.1_g000017 Rmu_sc0000308.1_g000018 Rmu_sc0003422.1_g000004 Rmu_sc0003776.1_g000026 Rmu_sc0005399.1_g000010 Rmu_sc0005399.1_g000012 Rmu_sc0009924.1_g000004
rosa_roxburghii Rroxscaffold_1G00053720 Rroxscaffold_1G00053730 Rroxscaffold_1G00054020 Rroxscaffold_2G00133470 Rroxscaffold_2G00133480 Rroxscaffold_3G00229920 Rroxscaffold_3G00229930 Rroxscaffold_3G00229940 Rroxscaffold_3G00245890 Rroxscaffold_3G00245900 Rroxscaffold_4G00317860 Rroxscaffold_5G00346620 Rroxscaffold_6G00396640 Rroxscaffold_6G00396930 Rroxscaffold_6G00396940 Rroxscaffold_6G00401510 Rroxscaffold_7G00201920 Rroxscaffold_7G00201930 Rroxscaffold_7G00201940
rosa_rugosa Rorug01G0116700 Rorug01G0116700 Rorug04G0072900 Rorug04G0073000 Rorug04G0112000 Rorug07G0258800
rosa_samantha Rh1AG123800 Rh1AG297000 Rh1DG057400 Rh1DG057500 Rh1DG129000 Rh2BG298000 Rh2BG471200 Rh2BG471300 Rh2BG471400 Rh2BG621400 Rh3CG245500 Rh3CG245600 Rh4DG157200 Rh4DG157300 Rh4DG157400 Rh4DG157500 Rh4DG157600 Rh4DG157700 Rh5DG402200 Rh6AG050900 Rh6AG051000 Rh6AG088400 Rh6AG088500 Rh6AG088600 Rh6AG088700 Rh6AG139800 Rh6AG139900 Rh6AG140000 Rh6CG077200 Rh6CG077300 Rh6CG077400 Rh6CG077500 Rh7CG225300 Rh7CG225400 Rh7CG225500 Rh7CG225600 Rh7CG267500 Rh7CG267600 Rh7CG267800 Rh7CG267900 Rh7CG338700 Rh7CG338800 Rh7CG338900 Rh7CG430900 Rh7CG431000 Rh7CG431100 Rh7DG219700 Rh7DG219800 Rh7DG219900 Rh7DG220000 Rh7DG257600 Rh7DG271500 Rh7DG367700 Rh7DG408600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1384
AccI GTMKAC 2 cut(s) 27, 129
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 1522
AcsI RAATTY 1 cut(s) 1099
AcuI CTGAAG 2 cut(s) 254, 1089
AdeI CACNNNGTG 1 cut(s) 1384
AfaI GTAC 3 cut(s) 43, 533, 858
AfiI CCNNNNNNNGG 1 cut(s) 1384
AflIII ACRYGT 1 cut(s) 1272
AhdI GACNNNNNGTC 1 cut(s) 442
AhlI ACTAGT 1 cut(s) 1109
AjiI CACGTC 1 cut(s) 220
AjuI GAANNNNNNNTTGG 2 cut(s) 1108, 1140
AluBI AGCT 3 cut(s) 372, 545, 1067
AluI AGCT 3 cut(s) 372, 545, 1067
Alw21I GWGCWC 2 cut(s) 296, 1044
Alw26I GTCTC 2 cut(s) 1073, 1263
AlwI GGATC 1 cut(s) 1522
AoxI GGCC 2 cut(s) 345, 414
ApeKI GCWGC 2 cut(s) 340, 704
ApoI RAATTY 1 cut(s) 1099
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 4 cut(s) 120, 346, 444, 553
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 3 cut(s) 146, 595, 688
AsuII TTCGAA 1 cut(s) 1301
AvaII GGWCC 3 cut(s) 120, 444, 553
BbsI GAAGAC 3 cut(s) 515, 873, 893
Bbv12I GWGCWC 2 cut(s) 296, 1044
BbvI GCAGC 2 cut(s) 327, 691
BciVI GTATCC 2 cut(s) 232, 473
BclI TGATCA 2 cut(s) 151, 1491
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 1073, 1263
BcuI ACTAGT 1 cut(s) 1109
BfaI CTAG 2 cut(s) 1110, 1193
BfmI CTRYAG 2 cut(s) 921, 1477
BfuI GTATCC 2 cut(s) 232, 473
BisI GCNGC 2 cut(s) 341, 705
BlpI GCTNAGC 1 cut(s) 844
BlsI GCNGC 2 cut(s) 342, 706
Bme1390I CCNGG 1 cut(s) 442
Bme18I GGWCC 3 cut(s) 120, 444, 553
BmeRI GACNNNNNGTC 1 cut(s) 442
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 4 cut(s) 120, 346, 444, 553
BmiI GGNNCC 3 cut(s) 121, 142, 554
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 2 cut(s) 391, 530
BmsI GCATC 3 cut(s) 67, 946, 1537
BmuI ACTGGG 2 cut(s) 391, 530
BpiI GAAGAC 3 cut(s) 515, 873, 893
Bpu10I CCTNAGC 1 cut(s) 302
Bpu1102I GCTNAGC 1 cut(s) 844
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 442
BsaI GGTCTC 1 cut(s) 1073
Bsc4I CCNNNNNNNGG 1 cut(s) 1384
Bse118I RCCGGY 1 cut(s) 1441
Bse1I ACTGG 4 cut(s) 386, 536, 1368, 1429
BseGI GGATG 4 cut(s) 82, 297, 613, 1151
BseLI CCNNNNNNNGG 1 cut(s) 1384
BseMII CTCAG 3 cut(s) 150, 734, 1509
BseNI ACTGG 4 cut(s) 386, 536, 1368, 1429
BseRI GAGGAG 1 cut(s) 1037
BseXI GCAGC 2 cut(s) 327, 691
BseYI CCCAGC 1 cut(s) 116
BsgI GTGCAG 1 cut(s) 786
Bsh1285I CGRYCG 1 cut(s) 640
BshFI GGCC 2 cut(s) 347, 416
BsiEI CGRYCG 1 cut(s) 640
BsiHKAI GWGCWC 2 cut(s) 296, 1044
BsiSI CCGG 2 cut(s) 442, 1442
BslFI GGGAC 2 cut(s) 230, 566
BslI CCNNNNNNNGG 1 cut(s) 1384
BsmAI GTCTC 2 cut(s) 1073, 1263
BsmFI GGGAC 2 cut(s) 230, 566
BsmI GAATGC 2 cut(s) 118, 1454
BsnI GGCC 2 cut(s) 347, 416
Bso31I GGTCTC 1 cut(s) 1073
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 2 cut(s) 296, 1044
Bsp143I GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
Bsp1720I GCTNAGC 1 cut(s) 844
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 347, 416
BspCNI CTCAG 3 cut(s) 151, 735, 1510
BspLI GGNNCC 3 cut(s) 121, 142, 554
BspPI GGATC 1 cut(s) 1522
BspT104I TTCGAA 1 cut(s) 1301
BspTNI GGTCTC 1 cut(s) 1073
BsrFI RCCGGY 1 cut(s) 1441
BsrI ACTGG 4 cut(s) 386, 536, 1368, 1429
BssAI RCCGGY 1 cut(s) 1441
BssMI GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
Bst4CI ACNGT 2 cut(s) 480, 1478
Bst6I CTCTTC 1 cut(s) 1065
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 3 cut(s) 345, 590, 1443
BstDEI CTNAG 5 cut(s) 159, 302, 743, 844, 1518
BstF5I GGATG 4 cut(s) 82, 297, 613, 1151
BstKTI GATC 6 cut(s) 154, 207, 640, 658, 1494, 1517
BstMAI GTCTC 2 cut(s) 1073, 1263
BstMBI GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
BstMCI CGRYCG 1 cut(s) 640
BstMWI GCNNNNNNNGC 2 cut(s) 369, 1206
BstNSI RCATGY 3 cut(s) 42, 1276, 1504
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 2 cut(s) 921, 1477
BstV1I GCAGC 2 cut(s) 327, 691
BstV2I GAAGAC 3 cut(s) 515, 873, 893
BstX2I RGATCY 1 cut(s) 1514
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 1 cut(s) 1514
BsuI GTATCC 2 cut(s) 232, 473
BsuRI GGCC 2 cut(s) 347, 416
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 4 cut(s) 82, 297, 613, 1151
BtsI GCAGTG 1 cut(s) 1511
BtsIMutI CAGTG 3 cut(s) 317, 1361, 1511
Cac8I GCNNGC 3 cut(s) 345, 590, 1443
Cfr10I RCCGGY 1 cut(s) 1441
Cfr13I GGNCC 4 cut(s) 120, 346, 444, 553
CseI GACGC 1 cut(s) 1206
Csp6I GTAC 3 cut(s) 42, 532, 857
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 9 cut(s) 39, 503, 557, 860, 1273, 1340, 1382, 1501, 1553
CviQI GTAC 3 cut(s) 42, 532, 857
DdeI CTNAG 5 cut(s) 159, 302, 743, 844, 1518
DpnI GATC 6 cut(s) 153, 206, 639, 657, 1493, 1516
DpnII GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
DraI TTTAAA 2 cut(s) 136, 378
DraIII CACNNNGTG 1 cut(s) 1384
DriI GACNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 1065
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 1065
Eco31I GGTCTC 1 cut(s) 1073
Eco47I GGWCC 3 cut(s) 120, 444, 553
Eco57I CTGAAG 2 cut(s) 254, 1089
EcoT22I ATGCAT 1 cut(s) 40
FaeI CATG 9 cut(s) 42, 506, 560, 863, 1276, 1343, 1385, 1504, 1556
FalI AAGNNNNNCTT 2 cut(s) 878, 910
FaqI GGGAC 2 cut(s) 230, 566
FatI CATG 9 cut(s) 38, 502, 556, 859, 1272, 1339, 1381, 1500, 1552
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 2 cut(s) 151, 1491
FblI GTMKAC 2 cut(s) 27, 129
Fnu4HI GCNGC 2 cut(s) 341, 705
FokI GGATG 4 cut(s) 89, 284, 620, 1158
Fsp4HI GCNGC 2 cut(s) 341, 705
FspBI CTAG 2 cut(s) 1110, 1193
GluI GCNGC 2 cut(s) 341, 705
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 2 cut(s) 347, 416
HapII CCGG 2 cut(s) 442, 1442
HgaI GACGC 1 cut(s) 1206
Hin1II CATG 9 cut(s) 42, 506, 560, 863, 1276, 1343, 1385, 1504, 1556
HincII GTYRAC 2 cut(s) 130, 253
HindII GTYRAC 2 cut(s) 130, 253
HindIII AAGCTT 1 cut(s) 543
HinfI GANTC 4 cut(s) 20, 437, 911, 1283
HpaII CCGG 2 cut(s) 442, 1442
HphI GGTGA 3 cut(s) 146, 595, 688
Hpy166II GTNNAC 6 cut(s) 19, 28, 130, 253, 285, 520
Hpy188III TCNNGA 3 cut(s) 778, 1019, 1399
Hpy8I GTNNAC 6 cut(s) 19, 28, 130, 253, 285, 520
HpyAV CCTTC 5 cut(s) 187, 229, 839, 1069, 1297
HpyCH4III ACNGT 2 cut(s) 480, 1478
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 1206
HpyF3I CTNAG 5 cut(s) 159, 302, 743, 844, 1518
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 9 cut(s) 42, 506, 560, 863, 1276, 1343, 1385, 1504, 1556
KroI GCCGGC 1 cut(s) 1441
KroNI GCCGGC 1 cut(s) 1443
Ksp22I TGATCA 2 cut(s) 151, 1491
Kzo9I GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
LmnI GCTCC 1 cut(s) 1047
Lsp1109I GCAGC 2 cut(s) 327, 691
LweI GCATC 3 cut(s) 67, 946, 1537
MaeI CTAG 2 cut(s) 1110, 1193
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 8 cut(s) 83, 977, 995, 1128, 1178, 1220, 1256, 1346
MalI GATC 6 cut(s) 153, 206, 639, 657, 1493, 1516
MboI GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
MflI RGATCY 1 cut(s) 1514
MhlI GDGCHC 2 cut(s) 296, 1044
MluCI AATT 2 cut(s) 1099, 1168
MlyI GAGTC 3 cut(s) 14, 431, 905
MmeI TCCRAC 1 cut(s) 702
MnlI CCTC 7 cut(s) 325, 406, 901, 1015, 1018, 1456, 1549
Mph1103I ATGCAT 1 cut(s) 40
MroNI GCCGGC 1 cut(s) 1441
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 5 cut(s) 135, 212, 377, 681, 1134
MslI CAYNNNNRTG 2 cut(s) 400, 1344
MspA1I CMGCKG 1 cut(s) 1067
MspI CCGG 2 cut(s) 442, 1442
MspR9I CCNGG 1 cut(s) 442
Mva1269I GAATGC 2 cut(s) 118, 1454
MwoI GCNNNNNNNGC 2 cut(s) 369, 1206
NaeI GCCGGC 1 cut(s) 1443
NciI CCSGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 670
NdeII GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
NgoMIV GCCGGC 1 cut(s) 1441
NlaIII CATG 9 cut(s) 42, 506, 560, 863, 1276, 1343, 1385, 1504, 1556
NlaIV GGNNCC 3 cut(s) 121, 142, 554
NmuCI GTSAC 4 cut(s) 83, 1128, 1178, 1346
NsiI ATGCAT 1 cut(s) 40
NspI RCATGY 3 cut(s) 42, 1276, 1504
NspV TTCGAA 1 cut(s) 1301
PciI ACATGT 1 cut(s) 1272
PctI GAATGC 2 cut(s) 118, 1454
PdiI GCCGGC 1 cut(s) 1443
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 1283
PflFI GACNNNGTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 1384
PkrI GCNGC 2 cut(s) 342, 706
Ple19I CGATCG 1 cut(s) 640
PleI GAGTC 3 cut(s) 14, 431, 905
PpsI GAGTC 3 cut(s) 14, 431, 905
PscI ACATGT 1 cut(s) 1272
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 121, 142, 554
PspPI GGNCC 4 cut(s) 120, 346, 444, 553
PsuI RGATCY 1 cut(s) 1514
PsyI GACNNNGTC 1 cut(s) 773
PvuI CGATCG 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 3 cut(s) 43, 533, 858
RsaNI GTAC 3 cut(s) 42, 532, 857
RseI CAYNNNNRTG 2 cut(s) 400, 1344
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 5 cut(s) 135, 212, 377, 681, 1134
SatI GCNGC 2 cut(s) 341, 705
Sau3AI GATC 6 cut(s) 151, 204, 637, 655, 1491, 1514
Sau96I GGNCC 4 cut(s) 120, 346, 444, 553
SchI GAGTC 3 cut(s) 14, 431, 905
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 2 cut(s) 296, 1044
SfaNI GCATC 3 cut(s) 67, 946, 1537
SfcI CTRYAG 2 cut(s) 921, 1477
SfuI TTCGAA 1 cut(s) 1301
SinI GGWCC 3 cut(s) 120, 444, 553
SmiMI CAYNNNNRTG 2 cut(s) 400, 1344
SmlI CTYRAG 1 cut(s) 778
SmoI CTYRAG 1 cut(s) 778
SpeI ACTAGT 1 cut(s) 1109
Sse9I AATT 2 cut(s) 1099, 1168
SsiI CCGC 1 cut(s) 456
SspMI CTAG 2 cut(s) 1110, 1193
StyD4I CCNGG 1 cut(s) 440
TaaI ACNGT 2 cut(s) 480, 1478
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 129, 1114, 1301
TasI AATT 2 cut(s) 1099, 1168
TatI WGTACW 2 cut(s) 41, 856
TfiI GAWTC 1 cut(s) 1283
Tru1I TTAA 5 cut(s) 135, 212, 377, 681, 1134
Tru9I TTAA 5 cut(s) 135, 212, 377, 681, 1134
TscAI CASTG 3 cut(s) 324, 1368, 1511
TseFI GTSAC 4 cut(s) 83, 1128, 1178, 1346
TseI GCWGC 2 cut(s) 340, 704
Tsp45I GTSAC 4 cut(s) 83, 1128, 1178, 1346
TspDTI ATGAA 4 cut(s) 573, 624, 959, 1265
TspGWI ACGGA 3 cut(s) 13, 72, 1158
TspRI CASTG 3 cut(s) 324, 1368, 1511
Tth111I GACNNNGTC 1 cut(s) 773
Van91I CCANNNNNTGG 1 cut(s) 1384
VpaK11BI GGWCC 3 cut(s) 120, 444, 553
XapI RAATTY 1 cut(s) 1099
XceI RCATGY 3 cut(s) 42, 1276, 1504
XcmI CCANNNNNNNNNTGG 1 cut(s) 543
XmiI GTMKAC 2 cut(s) 27, 129
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 2 cut(s) 1110, 1193
Zsp2I ATGCAT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.