MD01G1087700.v1.1

D-arabinono-1,4-lactone oxidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000215
Physical Location & Seq
Reverse (-)
19720310 .. 19725384
5075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1087700.v1.1.491

Sequence Viewer

Length: 2865 bp
ATGAGAGAAAATGATGTTGGTGAAGAGCCACAAAATGTTGTTGGGGAGTCTCATGATCATGAAAATTGTAGTGATTTAGAAGAAAATGTTGGTGATGAGTCTCAAGACCATGAAAATGGTGAAATGAGAGACTTGCTTGTAGAAAAGGGGCCAATTCGAGAAACTAATCTCACTTATCCTAAGGACAAACTCTTTAGAAAATTTTCCTCACACTACTATGATCGAAAATTACTAACAGGTGACATTTATGATAGGAAATGGCTCGTGTACTCAAAAGAGTTGGATAAAGTCTTTTGCTTTTGTTGTAAATTGTTTAAAACAATTGCCTCAAAAAGTGAGTTAGCAAAAGATGGAATTAGTAATTGGAGACATCTTGGTGTGAAACTTGACCAACATGAAAAGAGTAAAGAGCATCTTACTAATTTGAAAACTTGGGTTGAGTTGAAAAGTAGATTGACAACAAATCAGACAATTGATAAAGAATTTCAAATGCGAATCAAGAAAGAGACAAATCATTGGAAACAAGTGATGCTTAGAATTATTGCTGTTGTGAAATGTCTTGCCATACATAATTTAGCATTTTGTGGAACAAATGAAAAACCTTATAAAGACTCTAATGGAAACTTCTTAGGTTTACTTGAAATGATTGCAGAGTTTGATCCAATAATGCAAAAGCATTTTCGACTCATTGAGAATAAAGAGATTCATCACCACTATTTGAGCCATAAAATTCAAAATGAGTTGATAGCTACTTTAGCTTCAAAAGTCAAAACCACAATCATTAAAAAGATTAACATGGTGAGCAAAAAATTACAATCTGAAGACATGCGTCTTAATGTTGTTGTAAAGGCATTGGAAGCACTTGTTACCTTTTTTGAAAACTACAGAGAAACTGGTTTTGCTTCTGCCATGCGTGATGCTAAAGAAATTGCACTTGAATCGGAAATTGACCCTGTTTTTCAAACTAAACGTCATAGACCTAGAAAAATACATCATGATGAAGTTGATGTTAATGAGAGGGGACAACAGTCTGCTGAAGAATCATTTAACAGATTATTTTCTTGTTATAGTGGATATTGCTCCTTCTCAACTGAAACACAGGTGTTGCAAATGATATTACCTGAAGAAGCATTTTTCTCTAATAACCCTTGGACATCCATAGAAATAGCAAACTTTGTAAAAGAAATTGACATGTGTCCTAATGTCTTGATTGCTTATCGTGTACGGTTGACTGTACCTGTGACCGTGGCATCTGCAGAAAGAAGTTTTTCAAAATTGAAATTATTAAAATCTTACTTGCGGACCACTATGACTCAAGATAGGCTAAATGGATTAGCAATCTTATGCATTGAAAATGATGAGATTGAAGACTTGGAGTATGATGATATAATTGATGATTTTGCTTCAAAAAATGCCAGAAGACAATTTCTTAAAGGCAGTGTGACATACCCTATTAACGTGGAAGAACTTCTTTCAATAGTGGCCAATGCAACAAAGTCCAAGACAAAAATGAAGGTTGCAACTCGTTTCTCTCATAGCATTCCAAAGCTGGTATGTCCAAGTGGGGAAGACGGGTTGATCATAAGCACCAAGAACCTCAATCATGTGGTGGAGATAGATGCCGAAGCAAAGACTATGACATTGGAGAGTGGTGTGACTCTTAGGCAGCTCATCAGTGAGGGTGCCAAGGCAGGGCTGGCCTTGCCTTATGCACCATATTGGTGGGGTTTAACCATTGGAGGCATATTGGGGACTGGGGCGCATGGCAGCACGTTATGGGGTAGGGGAAGCTCGGTTCATGATTACGTGGTAGGACTTACAATTGTGAGTCCAGGAGGTTTTGAAGATGGTTACGTCAAGGTCAGGAAGCTCAATGATAGTGATCAAGATCTTAATGCAGCCAAAGTCTCACTTGGAGTCCTTGGAGTAATTTCACAGCACAAGGTAGTCTATCGGATGGATGATCGTGTTTCCTCGGATATGTCTGGCAATGGCTTATATGACTCCATAATTTTCCGAGCCACATCTCCACTTACGCTTGCAATTATCAGAACCACAGAAGAGATTCAGGAATCCACGAGGGATGCTTATGGGAAATGTCTTACTGCAAATTTATTTACATATCTTCTCATAAACAGTGCCTATGGTTTAACAAACAATGGTTTGAGCTTTACTGGCTACCCTATTATTGGATATCAGCACCGACTTCAATCATCTGGGACTTGCCAAGATAGTCTTGATACTAATACATCATGTGGATGGGATCCCAAAATCAAGGGTCAATTCTTTTACCAAACATCATTCAGCATCAGCCTGTCTATGGCTAAAAACTTCATCCAAGATGTGCAAAACCCAGTTGAGATTGAACCCAAGGCCTTGTGTGGAGTAGAAACATACAATGGAATCCTAATGCGCTATGTCACTGCCTCAAGTGCTTACCTTGGCAAGCAAGAAAATGCAATCGACTTCAATATCACATATTATCGAAGCAAAGACCCCATGACTCCTAGGCTTTACGAAGGCATACTCAAAGAAGTCGAGCAAATCGCGATGTTTAAATATGGAGCGTTGCCCCATTGGGGAAAAAATCGGAACGTGGCCTTTGATGGAGTGATCAACAAGTACAAGGGTGTCAAAGAGTTTTTGAGGGTCAAAGATGTTTATGATCCGATGGGGCTCTTCTCTAGTGAATGGACAGATCAAGTTTTTGGATTTAAGCGTGGGGTGAGTGTAGTGAAAGAAGGATGTGCACTAGAAGGGTTGTGCATTTGCTCACAAGACATTCATTGTGCCCCAAGAAAGGGCTATTTCTGTAGACCTGGCAAAGTCTCTGAAGATGCTAGGGTTTGTACTCTTGTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ALO FAD_binding_4

Protein Analysis

955

Amino Acids

108.05

Weight (kDa)

6.7

Isoelectric Point (pI)

30.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 394 - 453 5.7e-12 hAT family C-terminal dimerisation region
FAD_binding_4 PF01565 481 - 613 1.2e-16 FAD binding domain
ALO PF04030 763 - 901 5.2e-11 D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd PF22906 913 - 951 2.2e-22 L-gulonolactone oxidase 2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35150 AT2G06500 AT2G16040 AT2G19960 AT2G19960 AT3G29638 AT3G29794 AT4G10200 AT4G10200 AT5G35475
fragaria_vesca FvH4_1g08455 FvH4_1g08761 FvH4_1g10851 FvH4_1g17271 FvH4_1g26762 FvH4_2g15031 FvH4_2g18762 FvH4_2g29241 FvH4_2g32571 FvH4_4g01531 FvH4_4g12942 FvH4_4g14421 FvH4_4g14431 FvH4_4g14434 FvH4_5g30891 FvH4_6g47641 FvH4_6g47642
malus_domestica MD01G1087700.v1.1 MD11G1104400.v1.1
pyrus_communis pycom05g13550 pycom11g18320
rosa_chinensis RchiOBHm_Chr1g0320361 RchiOBHm_Chr1g0321011 RchiOBHm_Chr1g0321921 RchiOBHm_Chr1g0377611 RchiOBHm_Chr1g0379061 RchiOBHm_Chr2g0106921 RchiOBHm_Chr2g0137341 RchiOBHm_Chr2g0139641 RchiOBHm_Chr2g0139651 RchiOBHm_Chr2g0144921 RchiOBHm_Chr2g0161851 RchiOBHm_Chr2g0172511 RchiOBHm_Chr3g0449931 RchiOBHm_Chr3g0452101 RchiOBHm_Chr3g0480551 RchiOBHm_Chr4g0427821 RchiOBHm_Chr4g0431861 RchiOBHm_Chr4g0435271 RchiOBHm_Chr5g0015121 RchiOBHm_Chr5g0031951 RchiOBHm_Chr5g0034331 RchiOBHm_Chr5g0059431 RchiOBHm_Chr6g0288531 RchiOBHm_Chr6g0308901 RchiOBHm_Chr7g0198801 RchiOBHm_Chr7g0198811 RchiOBHm_Chr7g0200001 RchiOBHm_Chr7g0205811 RchiOBHm_Chr7g0230921 RchiOBHm_Chr7g0233981
rosa_multiflora Rmu_sc0000475.1_g000004 Rmu_sc0000483.1_g000021 Rmu_sc0000551.1_g000007 Rmu_sc0000761.1_g000023 Rmu_sc0000898.1_g000072 Rmu_sc0001921.1_g000031 Rmu_sc0002804.1_g000009 Rmu_sc0002880.1_g000005 Rmu_sc0003207.1_g000046 Rmu_sc0003914.1_g000010 Rmu_sc0004165.1_g000089 Rmu_sc0004209.1_g000001 Rmu_sc0004402.1_g000017 Rmu_sc0004443.1_g000001 Rmu_sc0005506.1_g000007 Rmu_sc0005506.1_g000009 Rmu_sc0006443.1_g000001 Rmu_sc0008191.1_g000019 Rmu_sc0008328.1_g000004 Rmu_sc0009489.1_g000004 Rmu_sc0010368.1_g000012 Rmu_sc0010368.1_g000014 Rmu_sc0011232.1_g000005 Rmu_sc0011630.1_g000006 Rmu_sc0014780.1_g000001 Rmu_sc0028257.1_g000002 Rmu_sc0029902.1_g000001 Rmu_ssc0000386.1_g000041 Rmu_ssc0000434.1_g000020
rosa_roxburghii Rroxscaffold_2G00084750 Rroxscaffold_2G00090380 Rroxscaffold_2G00108310 Rroxscaffold_3G00235050 Rroxscaffold_3G00252220 Rroxscaffold_4G00323580 Rroxscaffold_5G00337920 Rroxscaffold_5G00370050 Rroxscaffold_7G00169180 Rroxscaffold_7G00200470
rosa_rugosa Rorug01G0156600.1 Rorug02G0305900 Rorug02G0362300 Rorug03G0111800 Rorug03G0273500 Rorug03G0340700 Rorug04G0196000 Rorug05G0251300 Rorug05G0465700 Rorug06G0031300 Rorug06G0170700 Rorug07G0226000 Rorug07G0263200
rosa_samantha Rh1CG203400 Rh1CG203500 Rh3AG212700 Rh3BG246300 Rh4BG318100 Rh6BG118800 Rh6DG105400 Rh7DG231500
rosa_wichuraiana Rw1G002740 Rw1G016740 Rw2G009190 Rw2G021330 Rw2G030170 Rw2G034650 Rw2G034990 Rw2G046190 Rw3G019490 Rw3G019500 Rw3G019510 Rw3G019990 Rw3G020480 Rw3G026090 Rw4G005490 Rw4G007090 Rw4G022920 Rw4G030140 Rw5G011030 Rw5G019330 Rw5G027260 Rw5G029990 Rw5G034080 Rw6G025080 Rw6G031390 Rw6G038690 Rw7G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AccB1I GGYRCC 1 cut(s) 1682
AccI GTMKAC 1 cut(s) 2815
AccII CGCG 1 cut(s) 2549
AciI CCGC 1 cut(s) 1300
AclWI GGATC 4 cut(s) 653, 2258, 2271, 2660
AcoI YGGCCR 1 cut(s) 1482
AcsI RAATTY 4 cut(s) 200, 482, 729, 2110
AcuI CTGAAG 4 cut(s) 840, 1056, 1143, 2853
AfaI GTAC 5 cut(s) 269, 1224, 1236, 2624, 2851
AfiI CCNNNNNNNGG 6 cut(s) 1692, 2189, 2320, 2579, 2800, 2801
AflIII ACRYGT 1 cut(s) 1191
AjnI CCWGG 2 cut(s) 1831, 2818
AjuI GAANNNNNNNTTGG 5 cut(s) 32, 72, 104, 346, 378
AloI GAACNNNNNNTCC 2 cut(s) 1779, 1811
AluBI AGCT 7 cut(s) 749, 758, 1549, 1669, 1791, 1870, 2169
AluI AGCT 7 cut(s) 749, 758, 1549, 1669, 1791, 1870, 2169
Alw21I GWGCWC 1 cut(s) 2752
Alw26I GTCTC 7 cut(s) 54, 105, 123, 361, 500, 1912, 2833
Alw44I GTGCAC 1 cut(s) 2748
AlwI GGATC 4 cut(s) 653, 2258, 2271, 2660
AoxI GGCC 5 cut(s) 149, 1482, 1698, 2373, 2598
ApaLI GTGCAC 1 cut(s) 2748
ApeKI GCWGC 3 cut(s) 1666, 1767, 1898
ApoI RAATTY 4 cut(s) 200, 482, 729, 2110
ArsI GACNNNNNNTTYG 2 cut(s) 955, 987
Asp700I GAANNNNTTC 4 cut(s) 202, 1267, 1467, 2064
AspA2I CCTAGG 1 cut(s) 2507
AspLEI GCGC 2 cut(s) 1762, 2415
AspS9I GGNCC 2 cut(s) 149, 1302
AsuHPI GGTGA 7 cut(s) 32, 104, 131, 251, 701, 811, 2737
AvaII GGWCC 1 cut(s) 1302
AvrII CCTAGG 1 cut(s) 2507
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 2 cut(s) 2752, 2794
BalI TGGCCA 1 cut(s) 1484
BamHI GGATCC 1 cut(s) 2263
BanI GGYRCC 1 cut(s) 1682
BanII GRGCYC 1 cut(s) 2679
BauI CACGAG 2 cut(s) 263, 2077
BbsI GAAGAC 4 cut(s) 828, 1374, 1426, 1575
Bbv12I GWGCWC 1 cut(s) 2752
BbvI GCAGC 3 cut(s) 1678, 1779, 1910
BccI CCATC 6 cut(s) 344, 1841, 1951, 2253, 2600, 2665
BcgI CGANNNNNNTGC 2 cut(s) 921, 955
BciT130I CCWGG 2 cut(s) 1833, 2820
BclI TGATCA 4 cut(s) 55, 1578, 1882, 2613
BcoDI GTCTC 7 cut(s) 54, 105, 123, 361, 500, 1912, 2833
BfaI CTAG 5 cut(s) 981, 2508, 2685, 2753, 2841
BfmI CTRYAG 3 cut(s) 883, 1254, 2812
BglII AGATCT 1 cut(s) 1888
BisI GCNGC 3 cut(s) 1667, 1768, 1899
BlnI CCTAGG 1 cut(s) 2507
BlsI GCNGC 3 cut(s) 1668, 1769, 1900
Bme1390I CCNGG 2 cut(s) 1833, 2820
Bme18I GGWCC 1 cut(s) 1302
BmgT120I GGNCC 2 cut(s) 149, 1302
BmiI GGNNCC 3 cut(s) 150, 1684, 2265
BmrFI CCNGG 2 cut(s) 1833, 2820
BmrI ACTGGG 2 cut(s) 1764, 2348
BmsI GCATC 8 cut(s) 421, 519, 907, 1259, 1609, 2074, 2316, 2827
BmuI ACTGGG 2 cut(s) 1764, 2348
BoxI GACNNNNGTC 3 cut(s) 828, 1027, 1194
BpiI GAAGAC 4 cut(s) 828, 1374, 1426, 1575
BpuEI CTTGAG 3 cut(s) 87, 1299, 2413
BsaAI YACGTR 1 cut(s) 1807
BsaBI GATNNNNATC 2 cut(s) 1881, 1887
BsaJI CCNNGG 8 cut(s) 1148, 1245, 1686, 1921, 1974, 2370, 2440, 2507
Bsc4I CCNNNNNNNGG 6 cut(s) 1692, 2189, 2320, 2579, 2800, 2801
Bse1I ACTGG 4 cut(s) 898, 1759, 2179, 2354
Bse21I CCTNAGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 1996
Bse8I GATNNNNATC 2 cut(s) 1881, 1887
BseBI CCWGG 2 cut(s) 1833, 2820
BseDI CCNNGG 8 cut(s) 1148, 1245, 1686, 1921, 1974, 2370, 2440, 2507
BseGI GGATG 7 cut(s) 1154, 1962, 1966, 2089, 2264, 2334, 2750
BseJI GATNNNNATC 2 cut(s) 1881, 1887
BseLI CCNNNNNNNGG 6 cut(s) 1692, 2189, 2320, 2579, 2800, 2801
BseMI GCAATG 1 cut(s) 1996
BseNI ACTGG 4 cut(s) 898, 1759, 2179, 2354
BseSI GKGCMC 2 cut(s) 2752, 2794
BseXI GCAGC 3 cut(s) 1678, 1779, 1910
Bsh1236I CGCG 1 cut(s) 2549
BshFI GGCC 5 cut(s) 151, 1484, 1700, 2375, 2600
BshNI GGYRCC 1 cut(s) 1682
BsiHKAI GWGCWC 1 cut(s) 2752
BslFI GGGAC 3 cut(s) 1035, 1765, 2233
BslI CCNNNNNNNGG 6 cut(s) 1692, 2189, 2320, 2579, 2800, 2801
BsmAI GTCTC 7 cut(s) 54, 105, 123, 361, 500, 1912, 2833
BsmFI GGGAC 3 cut(s) 1035, 1765, 2233
BsmI GAATGC 1 cut(s) 1539
BsnI GGCC 5 cut(s) 151, 1484, 1700, 2375, 2600
Bsp1286I GDGCHC 3 cut(s) 2679, 2752, 2794
Bsp68I TCGCGA 1 cut(s) 2549
BspACI CCGC 1 cut(s) 1300
BspANI GGCC 5 cut(s) 151, 1484, 1700, 2375, 2600
BspFNI CGCG 1 cut(s) 2549
BspHI TCATGA 4 cut(s) 52, 58, 994, 1798
BspLI GGNNCC 3 cut(s) 150, 1684, 2265
BspMAI CTGCAG 1 cut(s) 1258
BspPI GGATC 4 cut(s) 653, 2258, 2271, 2660
BspQI GCTCTTC 2 cut(s) 18, 2684
BspT107I GGYRCC 1 cut(s) 1682
BsrDI GCAATG 1 cut(s) 1996
BsrI ACTGG 4 cut(s) 898, 1759, 2179, 2354
BssECI CCNNGG 8 cut(s) 1148, 1245, 1686, 1921, 1974, 2370, 2440, 2507
BssSI CACGAG 2 cut(s) 263, 2077
BssT1I CCWWGG 6 cut(s) 1148, 1686, 1921, 2370, 2440, 2507
Bst2BI CACGAG 2 cut(s) 263, 2077
Bst2UI CCWGG 2 cut(s) 1833, 2820
Bst4CI ACNGT 5 cut(s) 1029, 1227, 1234, 1246, 2138
Bst6I CTCTTC 3 cut(s) 18, 2055, 2684
BstBAI YACGTR 1 cut(s) 1807
BstC8I GCNNGC 3 cut(s) 1698, 2040, 2447
BstDEI CTNAG 4 cut(s) 180, 533, 628, 1661
BstDSI CCRYGG 1 cut(s) 1245
BstF5I GGATG 7 cut(s) 1154, 1962, 1966, 2089, 2264, 2334, 2750
BstFNI CGCG 1 cut(s) 2549
BstHHI GCGC 2 cut(s) 1762, 2415
BstMAI GTCTC 7 cut(s) 54, 105, 123, 361, 500, 1912, 2833
BstMWI GCNNNNNNNGC 6 cut(s) 755, 857, 1697, 1702, 2175, 2432
BstNI CCWGG 2 cut(s) 1833, 2820
BstNSI RCATGY 2 cut(s) 829, 1195
BstPAI GACNNNNGTC 3 cut(s) 828, 1027, 1194
BstSCI CCNGG 2 cut(s) 1831, 2818
BstSFI CTRYAG 3 cut(s) 883, 1254, 2812
BstSLI GKGCMC 2 cut(s) 2752, 2794
BstUI CGCG 1 cut(s) 2549
BstV1I GCAGC 3 cut(s) 1678, 1779, 1910
BstV2I GAAGAC 4 cut(s) 828, 1374, 1426, 1575
BstX2I RGATCY 2 cut(s) 1888, 2263
BstXI CCANNNNNNTGG 2 cut(s) 116, 1722
BstYI RGATCY 2 cut(s) 1888, 2263
Bsu36I CCTNAGG 1 cut(s) 180
BsuRI GGCC 5 cut(s) 151, 1484, 1700, 2375, 2600
BtgI CCRYGG 1 cut(s) 1245
BtgZI GCGATG 1 cut(s) 2564
BtsCI GGATG 7 cut(s) 1154, 1962, 1966, 2089, 2264, 2334, 2750
BtsI GCAGTG 2 cut(s) 1444, 2421
BtsIMutI CAGTG 4 cut(s) 1444, 1681, 2143, 2421
BtuMI TCGCGA 1 cut(s) 2549
Cac8I GCNNGC 3 cut(s) 1698, 2040, 2447
CciI TCATGA 4 cut(s) 52, 58, 994, 1798
CfoI GCGC 2 cut(s) 1762, 2415
Cfr13I GGNCC 2 cut(s) 149, 1302
CseI GACGC 1 cut(s) 818
Csp6I GTAC 5 cut(s) 268, 1223, 1235, 2623, 2850
CviQI GTAC 5 cut(s) 268, 1223, 1235, 2623, 2850
DdeI CTNAG 4 cut(s) 180, 533, 628, 1661
DraI TTTAAA 2 cut(s) 316, 2557
EaeI YGGCCR 1 cut(s) 1482
Eam1104I CTCTTC 3 cut(s) 18, 2055, 2684
EarI CTCTTC 3 cut(s) 18, 2055, 2684
Eco130I CCWWGG 6 cut(s) 1148, 1686, 1921, 2370, 2440, 2507
Eco147I AGGCCT 1 cut(s) 2375
Eco24I GRGCYC 1 cut(s) 2679
Eco32I GATATC 1 cut(s) 2195
Eco47I GGWCC 1 cut(s) 1302
Eco57I CTGAAG 4 cut(s) 840, 1056, 1143, 2853
Eco81I CCTNAGG 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 1831, 2818
EcoRV GATATC 1 cut(s) 2195
EcoT14I CCWWGG 6 cut(s) 1148, 1686, 1921, 2370, 2440, 2507
EcoT22I ATGCAT 1 cut(s) 1349
EcoT38I GRGCYC 1 cut(s) 2679
ErhI CCWWGG 6 cut(s) 1148, 1686, 1921, 2370, 2440, 2507
FaqI GGGAC 3 cut(s) 1035, 1765, 2233
FbaI TGATCA 4 cut(s) 55, 1578, 1882, 2613
FblI GTMKAC 1 cut(s) 2815
Fnu4HI GCNGC 3 cut(s) 1667, 1768, 1899
FokI GGATG 7 cut(s) 1141, 1969, 1973, 2096, 2271, 2321, 2757
FriOI GRGCYC 1 cut(s) 2679
Fsp4HI GCNGC 3 cut(s) 1667, 1768, 1899
FspBI CTAG 5 cut(s) 981, 2508, 2685, 2753, 2841
GlaI GCGC 2 cut(s) 1761, 2414
GluI GCNGC 3 cut(s) 1667, 1768, 1899
HaeIII GGCC 5 cut(s) 151, 1484, 1700, 2375, 2600
HgaI GACGC 1 cut(s) 818
HhaI GCGC 2 cut(s) 1762, 2415
Hin6I GCGC 2 cut(s) 1760, 2413
HinP1I GCGC 2 cut(s) 1760, 2413
HincII GTYRAC 1 cut(s) 1230
HindII GTYRAC 1 cut(s) 1230
HphI GGTGA 7 cut(s) 32, 104, 131, 251, 701, 811, 2737
Hpy166II GTNNAC 7 cut(s) 268, 635, 1223, 1230, 2750, 2816, 2860
Hpy8I GTNNAC 7 cut(s) 268, 635, 1223, 1230, 2750, 2816, 2860
HpyAV CCTTC 5 cut(s) 1093, 1507, 2513, 2735, 2750
HpyCH4III ACNGT 5 cut(s) 1029, 1227, 1234, 1246, 2138
HpyCH4IV ACGT 6 cut(s) 970, 1458, 1772, 1806, 1854, 2595
HpyF10VI GCNNNNNNNGC 6 cut(s) 755, 857, 1697, 1702, 2175, 2432
HpyF3I CTNAG 4 cut(s) 180, 533, 628, 1661
HpySE526I ACGT 6 cut(s) 970, 1458, 1772, 1806, 1854, 2595
HspAI GCGC 2 cut(s) 1760, 2413
Ksp22I TGATCA 4 cut(s) 55, 1578, 1882, 2613
LguI GCTCTTC 2 cut(s) 18, 2684
LmnI GCTCC 2 cut(s) 1085, 2564
Lsp1109I GCAGC 3 cut(s) 1678, 1779, 1910
LweI GCATC 8 cut(s) 421, 519, 907, 1259, 1609, 2074, 2316, 2827
MaeI CTAG 5 cut(s) 981, 2508, 2685, 2753, 2841
MaeII ACGT 6 cut(s) 970, 1458, 1772, 1806, 1854, 2595
MaeIII GTNAC 7 cut(s) 239, 865, 1240, 1441, 1654, 1850, 2419
MfeI CAATTG 3 cut(s) 321, 471, 1821
MflI RGATCY 2 cut(s) 1888, 2263
MhlI GDGCHC 3 cut(s) 2679, 2752, 2794
MlsI TGGCCA 1 cut(s) 1484
MluNI TGGCCA 1 cut(s) 1484
MmeI TCCRAC 1 cut(s) 261
Mox20I TGGCCA 1 cut(s) 1484
Mph1103I ATGCAT 1 cut(s) 1349
MroXI GAANNNNTTC 4 cut(s) 202, 1267, 1467, 2064
MscI TGGCCA 1 cut(s) 1484
MslI CAYNNNNRTG 7 cut(s) 57, 114, 216, 375, 996, 1720, 2257
Msp20I TGGCCA 1 cut(s) 1484
MspR9I CCNGG 2 cut(s) 1833, 2820
MunI CAATTG 3 cut(s) 321, 471, 1821
Mva1269I GAATGC 1 cut(s) 1539
MvaI CCWGG 2 cut(s) 1833, 2820
MvnI CGCG 1 cut(s) 2549
MwoI GCNNNNNNNGC 6 cut(s) 755, 857, 1697, 1702, 2175, 2432
NlaIV GGNNCC 3 cut(s) 150, 1684, 2265
NmuCI GTSAC 5 cut(s) 239, 1240, 1441, 1654, 2419
NruI TCGCGA 1 cut(s) 2549
NsiI ATGCAT 1 cut(s) 1349
NspI RCATGY 2 cut(s) 829, 1195
PagI TCATGA 4 cut(s) 52, 58, 994, 1798
PceI AGGCCT 1 cut(s) 2375
PciI ACATGT 1 cut(s) 1191
PciSI GCTCTTC 2 cut(s) 18, 2684
PctI GAATGC 1 cut(s) 1539
PdmI GAANNNNTTC 4 cut(s) 202, 1267, 1467, 2064
PfeI GAWTC 7 cut(s) 495, 703, 938, 1040, 2065, 2072, 2403
PfoI TCCNGGA 1 cut(s) 1831
PkrI GCNGC 3 cut(s) 1668, 1769, 1900
Ppu21I YACGTR 1 cut(s) 1807
PscI ACATGT 1 cut(s) 1191
PshAI GACNNNNGTC 3 cut(s) 828, 1027, 1194
PsiI TTATAA 1 cut(s) 606
Psp6I CCWGG 2 cut(s) 1831, 2818
PspGI CCWGG 2 cut(s) 1831, 2818
PspN4I GGNNCC 3 cut(s) 150, 1684, 2265
PspPI GGNCC 2 cut(s) 149, 1302
PstI CTGCAG 1 cut(s) 1258
PsuI RGATCY 2 cut(s) 1888, 2263
RruI TCGCGA 1 cut(s) 2549
RsaI GTAC 5 cut(s) 269, 1224, 1236, 2624, 2851
RsaNI GTAC 5 cut(s) 268, 1223, 1235, 2623, 2850
RseI CAYNNNNRTG 7 cut(s) 57, 114, 216, 375, 996, 1720, 2257
SapI GCTCTTC 2 cut(s) 18, 2684
SatI GCNGC 3 cut(s) 1667, 1768, 1899
Sau96I GGNCC 2 cut(s) 149, 1302
ScrFI CCNGG 2 cut(s) 1833, 2820
SduI GDGCHC 3 cut(s) 2679, 2752, 2794
SfaNI GCATC 8 cut(s) 421, 519, 907, 1259, 1609, 2074, 2316, 2827
SfcI CTRYAG 3 cut(s) 883, 1254, 2812
SinI GGWCC 1 cut(s) 1302
SmiMI CAYNNNNRTG 7 cut(s) 57, 114, 216, 375, 996, 1720, 2257
SmlI CTYRAG 3 cut(s) 102, 1314, 2428
SmoI CTYRAG 3 cut(s) 102, 1314, 2428
SseBI AGGCCT 1 cut(s) 2375
SsiI CCGC 1 cut(s) 1300
SspMI CTAG 5 cut(s) 981, 2508, 2685, 2753, 2841
StuI AGGCCT 1 cut(s) 2375
StyD4I CCNGG 2 cut(s) 1831, 2818
StyI CCWWGG 6 cut(s) 1148, 1686, 1921, 2370, 2440, 2507
TaaI ACNGT 5 cut(s) 1029, 1227, 1234, 1246, 2138
TaiI ACGT 6 cut(s) 973, 1461, 1775, 1809, 1857, 2598
TaqI TCGA 6 cut(s) 157, 223, 682, 2463, 2485, 2538
TatI WGTACW 3 cut(s) 267, 2622, 2849
TfiI GAWTC 7 cut(s) 495, 703, 938, 1040, 2065, 2072, 2403
TscAI CASTG 4 cut(s) 1444, 1681, 2143, 2428
TseFI GTSAC 5 cut(s) 239, 1240, 1441, 1654, 2419
TseI GCWGC 3 cut(s) 1666, 1767, 1898
Tsp45I GTSAC 5 cut(s) 239, 1240, 1441, 1654, 2419
TspRI CASTG 4 cut(s) 1444, 1681, 2143, 2428
VneI GTGCAC 1 cut(s) 2748
VpaK11BI GGWCC 1 cut(s) 1302
XapI RAATTY 4 cut(s) 200, 482, 729, 2110
XceI RCATGY 2 cut(s) 829, 1195
XcmI CCANNNNNNNNNTGG 1 cut(s) 1693
XmaJI CCTAGG 1 cut(s) 2507
XmiI GTMKAC 1 cut(s) 2815
XmnI GAANNNNTTC 4 cut(s) 202, 1267, 1467, 2064
XspI CTAG 5 cut(s) 981, 2508, 2685, 2753, 2841
Zsp2I ATGCAT 1 cut(s) 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.