Rw3G019990

Domain of unknown function (DUF4371)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000215
Physical Location & Seq
Reverse (-)
23188668 .. 23191190
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019990.1

Sequence Viewer

Length: 2523 bp
ATGCCTCCTAAAAGAAAATATTTATCCGGATATGCAAAACGCTTAAAGAAGAAAAAAGATGTAGAGTTGGTTAACTCTTTAAGGGGAAGTCTTGATAGGTTCATTACTAGGGAACCAGAAGGTTTGGGTGGACATTTAGTTAATGAAGATGCAGAAGAGCACACTGATGGGGAAGATAATGTGGAAGAGCATATTGATAGGGAAGATAATGTGGAAGAGCCCACTGATGGTAATGTAGTTGCTGATAATTTGGGTCATGATGAAAATGAAAATGGTACAGGACATAATGATAATGTGAATGATGCTTCTGTGAATATTTTTGACCCAAGAAATTGGGATAGTTTGGATAAAAAGTTGATAGATTTGTTGGTAGAGAAGGGTCCTCTTAGAGATCTTTCAATAGAAAATGGTCCTCCAGACAAGTTCAATAGACGTTTCAGTTCAAAATTCTACACTCGGTTTTTAGGAAATGGAGAGAAATATGATAGAGAGTGGCTAGTGTACTCAAGGGAGCTTGATAAGGTTTTTTGTTTTTGTTGCAAGTTGTTTAAGAGGGGGCCAATGCGAGGTCAGTTACCGAATGATGGGTACAATGATTGGGGTCACCTTAGTAAAAGACTTAAGGAGCATGAAATGAGCACTGATCATCTCATTAATGCAAATACTTGGACTGATTTGCGTCTTAGATTGCAGAGGCATGAAACCATTGATAAAGCTTTTCAAGACCAGATTAGGAAAGAGAAAGAACACTGGAGGAATGTGCTGAAAAGAATAATTGCAATTGTCAAATTTCTTGCTAAAAATAACTTAGCTTTCCGTGGAACTAATGAGAGGCTTTATGAACCTAATAATGGACACTTTTTGGGTCTAGTTGAAATGGTGGCAGAATGTGAAGAAGTGATGGAGGAGCATTGTAGACGCATAAATAGTTCTGAAATTCATCATCACTATCTTGGTCATAACATTCAGAATGAGTTGCTGCATATGATAGCTATTGAAATCAAAGCTGAAATCATAAAAAAAATTAAAGATGCTGAATATTTCTCTGTTATTCTTGATTGCACACCAGATATTAGTCACCAAGAGCAGATGTCATTGATACTTAGATGTGTGAATGCTTCAAAGAGTCCAATACAAATTGAAGAGTTCTTTGTGGAGTTTTTAAATGTACATGACACTTCTGGTCAGGGACTATTTGAAGAGCTGCAGGCTGTGGTACAATCATTGGATCTTGATATTAACAATGTGAGGGGACAAGGATATGACAACGGGTCAAATATGAAAGGCAAACACCAAGGGGTGCAAAAAAAATTGTTAGATATCAATCCTAGGGCATTATACACTCCGTGTGGTTGTCATTGTTTAAACTTAACTCTTTGTGATGTGGCAAATTCTTGTGGGAAAGCAGTGGATTTTTTTGGAATATTACAACGTCTTTATACCTTGTTTGCAGGTTCTACAAAGCGATGGCAGGTTTTGAAGGACAACCTAAAAGGATTCACTCTCAAATCATTATCCACTACGCGTTGGGAGAGTCGTATTGAAAGTGTTAAGCCTCTACGATTCAACGCCGTTGAAATAAGAGAAGCATTACTTCAATTGGCAGAAATTGACAAGGATCCCAAAATTAAAAGTGGTGCAAAGTCAATAGCAACTAATGATCTGGGAGACTTTGAGTTTTTGCTAAGCATGGTGATTTGGTATGAAATTTTGTTTGCTTTTAATAAGGTTAGCAAAGTGCTGCAGTCTCATGATATGCAAATTAGTGTTGCTATAGCTGAAGTAAAAGGTCTAATTGCTTGGCTTGATAAGTTTAGAGAAAATGGCTTCACAGATGCAATAATTACTGCTAAAGAAATTGCTGTTGCATTAGATGTTGATCCTGTTTTTCCTGAAAAGCGTCAAATTCAAAGAAAAAGATTTTTTGATGAAAGCGGCAGTGAGCCATCTCCATCATCTTCAAGTGAGGAATCTTTTAGACTACATTATTTCTTATATATTATAGATCAAGCTAAGGGGTCATTAAATAGAAGGTTTGAACAATACCAACGTTATGATGATATCTTTGGATTTTTATTCACTTCTGAAACTTTGAATTCCTTGAATGATAATGATTTGAAAGCTGCTTGTATTCATCTTGAGACTGTTTTGAGATATGGAGAGAGTTCAGATGTTGATGGGGAAGACATGTTTAGAGAGCTAAAACTCTTAAGGGAGATATTACCCAAACAGAAGATGACAGCTAGTGATATATTGAATTTCTTACTGGAAAGGAATAGCTGCCCAGTTGTTAGACTTGCATATCGAATATTGTTAACTGTGCCTGTTACTGTTGCTTCAGCTGAGAGGAGTTTTTCCAAGTTAAAGTTATTGAAGTCTTATTTGCGATCAACTATGTCACAAGAAAGGCTAAATGGACTTGCTTTGATTTCGATTGAGAATGAATATCTTGGAAAAATCAATTGTGATAAGCTAATTGATCAGTTTGCAGGGAAGAAGGCAAGGAGATGGATTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4371 Dimer_Tnp_hAT

Protein Analysis

840

Amino Acids

96.78

Weight (kDa)

6.17

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4371 PF14291 229 - 433 8e-21 Domain of unknown function (DUF4371)
Dimer_Tnp_hAT PF05699 742 - 815 3.9e-14 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35150 AT2G06500 AT2G16040 AT2G19960 AT2G19960 AT3G29638 AT3G29794 AT4G10200 AT4G10200 AT5G35475
fragaria_vesca FvH4_1g08455 FvH4_1g08761 FvH4_1g10851 FvH4_1g17271 FvH4_1g26762 FvH4_2g15031 FvH4_2g18762 FvH4_2g29241 FvH4_2g32571 FvH4_4g01531 FvH4_4g12942 FvH4_4g14421 FvH4_4g14431 FvH4_4g14434 FvH4_5g30891 FvH4_6g47641 FvH4_6g47642
malus_domestica MD01G1087700.v1.1 MD11G1104400.v1.1
pyrus_communis pycom05g13550 pycom11g18320
rosa_chinensis RchiOBHm_Chr1g0320361 RchiOBHm_Chr1g0321011 RchiOBHm_Chr1g0321921 RchiOBHm_Chr1g0377611 RchiOBHm_Chr1g0379061 RchiOBHm_Chr2g0106921 RchiOBHm_Chr2g0137341 RchiOBHm_Chr2g0139641 RchiOBHm_Chr2g0139651 RchiOBHm_Chr2g0144921 RchiOBHm_Chr2g0161851 RchiOBHm_Chr2g0172511 RchiOBHm_Chr3g0449931 RchiOBHm_Chr3g0452101 RchiOBHm_Chr3g0480551 RchiOBHm_Chr4g0427821 RchiOBHm_Chr4g0431861 RchiOBHm_Chr4g0435271 RchiOBHm_Chr5g0015121 RchiOBHm_Chr5g0031951 RchiOBHm_Chr5g0034331 RchiOBHm_Chr5g0059431 RchiOBHm_Chr6g0288531 RchiOBHm_Chr6g0308901 RchiOBHm_Chr7g0198801 RchiOBHm_Chr7g0198811 RchiOBHm_Chr7g0200001 RchiOBHm_Chr7g0205811 RchiOBHm_Chr7g0230921 RchiOBHm_Chr7g0233981
rosa_multiflora Rmu_sc0000475.1_g000004 Rmu_sc0000483.1_g000021 Rmu_sc0000551.1_g000007 Rmu_sc0000761.1_g000023 Rmu_sc0000898.1_g000072 Rmu_sc0001921.1_g000031 Rmu_sc0002804.1_g000009 Rmu_sc0002880.1_g000005 Rmu_sc0003207.1_g000046 Rmu_sc0003914.1_g000010 Rmu_sc0004165.1_g000089 Rmu_sc0004209.1_g000001 Rmu_sc0004402.1_g000017 Rmu_sc0004443.1_g000001 Rmu_sc0005506.1_g000007 Rmu_sc0005506.1_g000009 Rmu_sc0006443.1_g000001 Rmu_sc0008191.1_g000019 Rmu_sc0008328.1_g000004 Rmu_sc0009489.1_g000004 Rmu_sc0010368.1_g000012 Rmu_sc0010368.1_g000014 Rmu_sc0011232.1_g000005 Rmu_sc0011630.1_g000006 Rmu_sc0014780.1_g000001 Rmu_sc0028257.1_g000002 Rmu_sc0029902.1_g000001 Rmu_ssc0000386.1_g000041 Rmu_ssc0000434.1_g000020
rosa_roxburghii Rroxscaffold_2G00084750 Rroxscaffold_2G00090380 Rroxscaffold_2G00108310 Rroxscaffold_3G00235050 Rroxscaffold_3G00252220 Rroxscaffold_4G00323580 Rroxscaffold_5G00337920 Rroxscaffold_5G00370050 Rroxscaffold_7G00169180 Rroxscaffold_7G00200470
rosa_rugosa Rorug01G0156600.1 Rorug02G0305900 Rorug02G0362300 Rorug03G0111800 Rorug03G0273500 Rorug03G0340700 Rorug04G0196000 Rorug05G0251300 Rorug05G0465700 Rorug06G0031300 Rorug06G0170700 Rorug07G0226000 Rorug07G0263200
rosa_samantha Rh1CG203400 Rh1CG203500 Rh3AG212700 Rh3BG246300 Rh4BG318100 Rh6BG118800 Rh6DG105400 Rh7DG231500
rosa_wichuraiana Rw1G002740 Rw1G016740 Rw2G009190 Rw2G021330 Rw2G030170 Rw2G034650 Rw2G034990 Rw2G046190 Rw3G019490 Rw3G019500 Rw3G019510 Rw3G019990 Rw3G020480 Rw3G026090 Rw4G005490 Rw4G007090 Rw4G022920 Rw4G030140 Rw5G011030 Rw5G019330 Rw5G027260 Rw5G029990 Rw5G034080 Rw6G025080 Rw6G031390 Rw6G038690 Rw7G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1442, 1462
AccI GTMKAC 1 cut(s) 916
AccII CGCG 1 cut(s) 1525
AccIII TCCGGA 1 cut(s) 26
AciI CCGC 1 cut(s) 1935
AclI AACGTT 1 cut(s) 2050
AclWI GGATC 4 cut(s) 1236, 1613, 1626, 1874
AcsI RAATTY 8 cut(s) 446, 788, 936, 1390, 1707, 1905, 2095, 2257
AcuI CTGAAG 2 cut(s) 1800, 2322
AdeI CACNNNGTG 1 cut(s) 1347
AfaI GTAC 5 cut(s) 277, 503, 590, 1170, 1218
AfiI CCNNNNNNNGG 4 cut(s) 227, 566, 584, 851
AflII CTTAAG 2 cut(s) 620, 2209
AflIII ACRYGT 2 cut(s) 1523, 2187
AhdI GACNNNNNGTC 1 cut(s) 1270
Alw21I GWGCWC 2 cut(s) 162, 641
Alw26I GTCTC 3 cut(s) 1662, 1752, 2135
AlwI GGATC 4 cut(s) 1236, 1613, 1626, 1874
Aor13HI TCCGGA 1 cut(s) 26
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 5 cut(s) 979, 1204, 1741, 2123, 2280
ApoI RAATTY 8 cut(s) 446, 788, 936, 1390, 1707, 1905, 2095, 2257
AseI ATTAAT 1 cut(s) 654
AspA2I CCTAGG 1 cut(s) 1328
AspS9I GGNCC 3 cut(s) 380, 410, 557
AsuHPI GGTGA 3 cut(s) 596, 1070, 1705
AvaII GGWCC 2 cut(s) 380, 410
AvrII CCTAGG 1 cut(s) 1328
BamHI GGATCC 1 cut(s) 1618
BanII GRGCYC 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 2190
Bbv12I GWGCWC 2 cut(s) 162, 641
BbvI GCAGC 5 cut(s) 966, 1191, 1728, 2110, 2267
BccI CCATC 9 cut(s) 161, 221, 578, 895, 1461, 1954, 1960, 2171, 2502
BceAI ACGGC 1 cut(s) 1556
BclI TGATCA 2 cut(s) 643, 2479
BcoDI GTCTC 3 cut(s) 1662, 1752, 2135
BfaI CTAG 5 cut(s) 108, 497, 869, 1329, 2244
BfmI CTRYAG 3 cut(s) 1205, 1742, 1773
BfrI CTTAAG 2 cut(s) 620, 2209
BfuAI ACCTGC 2 cut(s) 1442, 1462
BglII AGATCT 1 cut(s) 391
BisI GCNGC 6 cut(s) 980, 1205, 1742, 1936, 2124, 2281
BlnI CCTAGG 1 cut(s) 1328
BlpI GCTNAGC 1 cut(s) 1685
BlsI GCNGC 6 cut(s) 981, 1206, 1743, 1937, 2125, 2282
Bme18I GGWCC 2 cut(s) 380, 410
BmeRI GACNNNNNGTC 1 cut(s) 1270
BmgT120I GGNCC 3 cut(s) 380, 410, 557
BmiI GGNNCC 4 cut(s) 114, 381, 558, 1620
BmrI ACTGGG 1 cut(s) 2279
BmsI GCATC 4 cut(s) 139, 292, 1021, 1825
BmuI ACTGGG 1 cut(s) 2279
BpiI GAAGAC 1 cut(s) 2190
BpmI CTGGAG 2 cut(s) 399, 772
Bpu10I CCTNAGC 1 cut(s) 2013
Bpu1102I GCTNAGC 1 cut(s) 1685
BpuEI CTTGAG 2 cut(s) 490, 2159
BsaBI GATNNNNATC 2 cut(s) 1323, 1878
BsaJI CCNNGG 3 cut(s) 817, 1294, 1328
BsaWI WCCGGW 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 566, 584, 851
Bse1I ACTGG 3 cut(s) 755, 2271, 2285
Bse8I GATNNNNATC 2 cut(s) 1323, 1878
BseAI TCCGGA 1 cut(s) 26
BseDI CCNNGG 3 cut(s) 817, 1294, 1328
BseJI GATNNNNATC 2 cut(s) 1323, 1878
BseLI CCNNNNNNNGG 4 cut(s) 227, 566, 584, 851
BseMII CTCAG 1 cut(s) 2334
BseNI ACTGG 3 cut(s) 755, 2271, 2285
BseRI GAGGAG 2 cut(s) 920, 2362
BseXI GCAGC 5 cut(s) 966, 1191, 1728, 2110, 2267
Bsh1236I CGCG 1 cut(s) 1525
BshFI GGCC 1 cut(s) 559
BsiHKAI GWGCWC 2 cut(s) 162, 641
BsiSI CCGG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 1203, 1266
BslI CCNNNNNNNGG 4 cut(s) 227, 566, 584, 851
BsmAI GTCTC 3 cut(s) 1662, 1752, 2135
BsmFI GGGAC 2 cut(s) 1203, 1266
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 1 cut(s) 559
Bsp1286I GDGCHC 3 cut(s) 162, 222, 641
Bsp13I TCCGGA 1 cut(s) 26
Bsp1407I TGTACA 1 cut(s) 1168
Bsp143I GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
Bsp1720I GCTNAGC 1 cut(s) 1685
BspACI CCGC 1 cut(s) 1935
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 1 cut(s) 2335
BspEI TCCGGA 1 cut(s) 26
BspFNI CGCG 1 cut(s) 1525
BspHI TCATGA 2 cut(s) 256, 1750
BspLI GGNNCC 4 cut(s) 114, 381, 558, 1620
BspMAI CTGCAG 2 cut(s) 1209, 1746
BspMI ACCTGC 2 cut(s) 1442, 1462
BspPI GGATC 4 cut(s) 1236, 1613, 1626, 1874
BspQI GCTCTTC 4 cut(s) 150, 180, 210, 1194
BspTI CTTAAG 2 cut(s) 620, 2209
BsrGI TGTACA 1 cut(s) 1168
BsrI ACTGG 3 cut(s) 755, 2271, 2285
BssECI CCNNGG 3 cut(s) 817, 1294, 1328
BssMI GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
BssT1I CCWWGG 2 cut(s) 1294, 1328
Bst4CI ACNGT 3 cut(s) 2146, 2320, 2332
Bst6I CTCTTC 5 cut(s) 150, 180, 210, 1137, 1194
BstAFI CTTAAG 2 cut(s) 620, 2209
BstAUI TGTACA 1 cut(s) 1168
BstC8I GCNNGC 1 cut(s) 1209
BstDEI CTNAG 8 cut(s) 386, 608, 683, 808, 1103, 1685, 2013, 2343
BstDSI CCRYGG 1 cut(s) 817
BstEII GGTNACC 1 cut(s) 602
BstFNI CGCG 1 cut(s) 1525
BstKTI GATC 9 cut(s) 394, 646, 1231, 1621, 1663, 1882, 2008, 2390, 2482
BstMAI GTCTC 3 cut(s) 1662, 1752, 2135
BstMBI GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
BstNSI RCATGY 1 cut(s) 2191
BstPI GGTNACC 1 cut(s) 602
BstSFI CTRYAG 3 cut(s) 1205, 1742, 1773
BstUI CGCG 1 cut(s) 1525
BstV1I GCAGC 5 cut(s) 966, 1191, 1728, 2110, 2267
BstV2I GAAGAC 1 cut(s) 2190
BstX2I RGATCY 3 cut(s) 391, 1228, 1618
BstXI CCANNNNNNTGG 1 cut(s) 333
BstYI RGATCY 3 cut(s) 391, 1228, 1618
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 817
BtgZI GCGATG 1 cut(s) 1480
BtsI GCAGTG 2 cut(s) 1413, 1945
BtsIMutI CAGTG 6 cut(s) 162, 222, 639, 748, 1413, 1945
BveI ACCTGC 2 cut(s) 1442, 1462
Cac8I GCNNGC 1 cut(s) 1209
CciI TCATGA 2 cut(s) 256, 1750
Cfr13I GGNCC 3 cut(s) 380, 410, 557
CseI GACGC 3 cut(s) 668, 927, 1889
Csp6I GTAC 5 cut(s) 276, 502, 589, 1169, 1217
CviAII CATG 7 cut(s) 257, 629, 698, 1172, 1690, 1751, 2188
CviQI GTAC 5 cut(s) 276, 502, 589, 1169, 1217
DdeI CTNAG 8 cut(s) 386, 608, 683, 808, 1103, 1685, 2013, 2343
DpnI GATC 9 cut(s) 393, 645, 1230, 1620, 1662, 1881, 2007, 2389, 2481
DpnII GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
DraI TTTAAA 2 cut(s) 1164, 1365
DraIII CACNNNGTG 1 cut(s) 1347
DriI GACNNNNNGTC 1 cut(s) 1270
Eam1104I CTCTTC 5 cut(s) 150, 180, 210, 1137, 1194
Eam1105I GACNNNNNGTC 1 cut(s) 1270
EarI CTCTTC 5 cut(s) 150, 180, 210, 1137, 1194
Eco130I CCWWGG 2 cut(s) 1294, 1328
Eco24I GRGCYC 1 cut(s) 222
Eco32I GATATC 2 cut(s) 1321, 2062
Eco47I GGWCC 2 cut(s) 380, 410
Eco57I CTGAAG 2 cut(s) 1800, 2322
Eco91I GGTNACC 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 380
EcoO65I GGTNACC 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 2095
EcoRV GATATC 2 cut(s) 1321, 2062
EcoT14I CCWWGG 2 cut(s) 1294, 1328
EcoT38I GRGCYC 1 cut(s) 222
ErhI CCWWGG 2 cut(s) 1294, 1328
FaeI CATG 7 cut(s) 260, 632, 701, 1175, 1693, 1754, 2191
FalI AAGNNNNNCTT 2 cut(s) 1578, 1610
FaqI GGGAC 2 cut(s) 1203, 1266
FatI CATG 7 cut(s) 256, 628, 697, 1171, 1689, 1750, 2187
FauNDI CATATG 1 cut(s) 984
FbaI TGATCA 2 cut(s) 643, 2479
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 6 cut(s) 980, 1205, 1742, 1936, 2124, 2281
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 6 cut(s) 980, 1205, 1742, 1936, 2124, 2281
FspBI CTAG 5 cut(s) 108, 497, 869, 1329, 2244
GluI GCNGC 6 cut(s) 980, 1205, 1742, 1936, 2124, 2281
GsuI CTGGAG 2 cut(s) 399, 772
HaeIII GGCC 1 cut(s) 559
HapII CCGG 1 cut(s) 27
HgaI GACGC 3 cut(s) 668, 927, 1889
Hin1II CATG 7 cut(s) 260, 632, 701, 1175, 1693, 1754, 2191
HincII GTYRAC 2 cut(s) 73, 2316
HindII GTYRAC 2 cut(s) 73, 2316
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 5 cut(s) 1126, 1497, 1534, 1563, 1970
HpaI GTTAAC 2 cut(s) 73, 2316
HpaII CCGG 1 cut(s) 27
HphI GGTGA 3 cut(s) 596, 1070, 1705
Hpy166II GTNNAC 5 cut(s) 73, 131, 502, 917, 2316
Hpy188I TCNGA 4 cut(s) 934, 969, 2086, 2170
Hpy8I GTNNAC 5 cut(s) 73, 131, 502, 917, 2316
HpyAV CCTTC 5 cut(s) 113, 370, 1474, 2025, 2491
HpyCH4III ACNGT 3 cut(s) 2146, 2320, 2332
HpyCH4IV ACGT 3 cut(s) 433, 1432, 2050
HpyF3I CTNAG 8 cut(s) 386, 608, 683, 808, 1103, 1685, 2013, 2343
HpySE526I ACGT 3 cut(s) 433, 1432, 2050
Hsp92II CATG 7 cut(s) 260, 632, 701, 1175, 1693, 1754, 2191
Kpn2I TCCGGA 1 cut(s) 26
Ksp22I TGATCA 2 cut(s) 643, 2479
KspAI GTTAAC 2 cut(s) 73, 2316
Kzo9I GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
LguI GCTCTTC 4 cut(s) 150, 180, 210, 1194
LmnI GCTCC 3 cut(s) 511, 625, 907
Lsp1109I GCAGC 5 cut(s) 966, 1191, 1728, 2110, 2267
LweI GCATC 4 cut(s) 139, 292, 1021, 1825
MaeI CTAG 5 cut(s) 108, 497, 869, 1329, 2244
MaeII ACGT 3 cut(s) 433, 1432, 2050
MaeIII GTNAC 5 cut(s) 573, 602, 1076, 2326, 2397
MalI GATC 9 cut(s) 393, 645, 1230, 1620, 1662, 1881, 2007, 2389, 2481
MboI GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
MfeI CAATTG 3 cut(s) 780, 1598, 2461
MflI RGATCY 3 cut(s) 391, 1228, 1618
MhlI GDGCHC 3 cut(s) 162, 222, 641
MluI ACGCGT 1 cut(s) 1523
MlyI GAGTC 2 cut(s) 1135, 1543
MroI TCCGGA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 2342
MspCI CTTAAG 2 cut(s) 620, 2209
MspI CCGG 1 cut(s) 27
MssI GTTTAAAC 1 cut(s) 1365
MunI CAATTG 3 cut(s) 780, 1598, 2461
Mva1269I GAATGC 1 cut(s) 1120
MvnI CGCG 1 cut(s) 1525
NdeI CATATG 1 cut(s) 984
NdeII GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
NlaIII CATG 7 cut(s) 260, 632, 701, 1175, 1693, 1754, 2191
NlaIV GGNNCC 4 cut(s) 114, 381, 558, 1620
NmuCI GTSAC 3 cut(s) 602, 1076, 2397
NspI RCATGY 1 cut(s) 2191
PagI TCATGA 2 cut(s) 256, 1750
PciI ACATGT 1 cut(s) 2187
PciSI GCTCTTC 4 cut(s) 150, 180, 210, 1194
PctI GAATGC 1 cut(s) 1120
PfeI GAWTC 3 cut(s) 1497, 1563, 1970
PkrI GCNGC 6 cut(s) 981, 1206, 1743, 1937, 2125, 2282
PleI GAGTC 2 cut(s) 1134, 1542
PmeI GTTTAAAC 1 cut(s) 1365
PpsI GAGTC 2 cut(s) 1134, 1542
PpuMI RGGWCCY 1 cut(s) 380
PscI ACATGT 1 cut(s) 2187
PshBI ATTAAT 1 cut(s) 654
Psp1406I AACGTT 1 cut(s) 2050
Psp5II RGGWCCY 1 cut(s) 380
PspEI GGTNACC 1 cut(s) 602
PspN4I GGNNCC 4 cut(s) 114, 381, 558, 1620
PspPI GGNCC 3 cut(s) 380, 410, 557
PspPPI RGGWCCY 1 cut(s) 380
PstI CTGCAG 2 cut(s) 1209, 1746
PsuI RGATCY 3 cut(s) 391, 1228, 1618
PvuII CAGCTG 1 cut(s) 2342
RsaI GTAC 5 cut(s) 277, 503, 590, 1170, 1218
RsaNI GTAC 5 cut(s) 276, 502, 589, 1169, 1217
RseI CAYNNNNRTG 1 cut(s) 165
SapI GCTCTTC 4 cut(s) 150, 180, 210, 1194
SatI GCNGC 6 cut(s) 980, 1205, 1742, 1936, 2124, 2281
Sau3AI GATC 9 cut(s) 391, 643, 1228, 1618, 1660, 1879, 2005, 2387, 2479
Sau96I GGNCC 3 cut(s) 380, 410, 557
SchI GAGTC 2 cut(s) 1135, 1543
SduI GDGCHC 3 cut(s) 162, 222, 641
SfaNI GCATC 4 cut(s) 139, 292, 1021, 1825
SfcI CTRYAG 3 cut(s) 1205, 1742, 1773
SinI GGWCC 2 cut(s) 380, 410
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 4 cut(s) 505, 620, 2138, 2209
SmoI CTYRAG 4 cut(s) 505, 620, 2138, 2209
SsiI CCGC 1 cut(s) 1935
SspI AATATT 5 cut(s) 20, 316, 1040, 1425, 2310
SspMI CTAG 5 cut(s) 108, 497, 869, 1329, 2244
StyI CCWWGG 2 cut(s) 1294, 1328
TaaI ACNGT 3 cut(s) 2146, 2320, 2332
TaiI ACGT 3 cut(s) 436, 1435, 2053
TaqI TCGA 2 cut(s) 2305, 2432
TatI WGTACW 2 cut(s) 501, 1168
TauI GCSGC 1 cut(s) 1938
TfiI GAWTC 3 cut(s) 1497, 1563, 1970
TscAI CASTG 6 cut(s) 169, 229, 646, 755, 1413, 1945
TseFI GTSAC 3 cut(s) 602, 1076, 2397
TseI GCWGC 5 cut(s) 979, 1204, 1741, 2123, 2280
Tsp45I GTSAC 3 cut(s) 602, 1076, 2397
TspGWI ACGGA 2 cut(s) 806, 1335
TspRI CASTG 6 cut(s) 169, 229, 646, 755, 1413, 1945
Vha464I CTTAAG 2 cut(s) 620, 2209
VpaK11BI GGWCC 2 cut(s) 380, 410
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 8 cut(s) 446, 788, 936, 1390, 1707, 1905, 2095, 2257
XceI RCATGY 1 cut(s) 2191
XmaJI CCTAGG 1 cut(s) 1328
XmiI GTMKAC 1 cut(s) 916
XspI CTAG 5 cut(s) 108, 497, 869, 1329, 2244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.