Domain of unknown function (DUF4371)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 542 |
| AcsI | RAATTY | 1 cut(s) | 362 |
| AfaI | GTAC | 2 cut(s) | 193, 419 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 482 |
| AgsI | TTSAA | 4 cut(s) | 315, 343, 360, 580 |
| AluBI | AGCT | 1 cut(s) | 535 |
| AluI | AGCT | 1 cut(s) | 535 |
| Alw21I | GWGCWC | 2 cut(s) | 108, 138 |
| AoxI | GGCC | 1 cut(s) | 473 |
| ApoI | RAATTY | 1 cut(s) | 362 |
| AspS9I | GGNCC | 3 cut(s) | 296, 326, 473 |
| AvaII | GGWCC | 2 cut(s) | 296, 326 |
| Bbv12I | GWGCWC | 2 cut(s) | 108, 138 |
| BccI | CCATC | 2 cut(s) | 137, 564 |
| BclI | TGATCA | 1 cut(s) | 541 |
| BfaI | CTAG | 1 cut(s) | 413 |
| BfuAI | ACCTGC | 1 cut(s) | 542 |
| BglII | AGATCT | 1 cut(s) | 307 |
| Bme18I | GGWCC | 2 cut(s) | 296, 326 |
| BmgT120I | GGNCC | 3 cut(s) | 296, 326, 473 |
| BmiI | GGNNCC | 2 cut(s) | 297, 474 |
| BmsI | GCATC | 1 cut(s) | 208 |
| BpmI | CTGGAG | 1 cut(s) | 315 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 482 |
| Bse3DI | GCAATG | 1 cut(s) | 154 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 482 |
| BseMI | GCAATG | 1 cut(s) | 154 |
| BshFI | GGCC | 1 cut(s) | 475 |
| BsiHKAI | GWGCWC | 2 cut(s) | 108, 138 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 482 |
| BsnI | GGCC | 1 cut(s) | 475 |
| Bsp1286I | GDGCHC | 2 cut(s) | 108, 138 |
| Bsp143I | GATC | 2 cut(s) | 307, 541 |
| BspANI | GGCC | 1 cut(s) | 475 |
| BspHI | TCATGA | 1 cut(s) | 172 |
| BspLI | GGNNCC | 2 cut(s) | 297, 474 |
| BspMI | ACCTGC | 1 cut(s) | 542 |
| BspQI | GCTCTTC | 2 cut(s) | 96, 126 |
| BsrDI | GCAATG | 1 cut(s) | 154 |
| BssMI | GATC | 2 cut(s) | 307, 541 |
| Bst6I | CTCTTC | 2 cut(s) | 96, 126 |
| BstKTI | GATC | 2 cut(s) | 310, 544 |
| BstMBI | GATC | 2 cut(s) | 307, 541 |
| BstX2I | RGATCY | 1 cut(s) | 307 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 249 |
| BstYI | RGATCY | 1 cut(s) | 307 |
| BsuRI | GGCC | 1 cut(s) | 475 |
| BtsIMutI | CAGTG | 2 cut(s) | 108, 138 |
| BveI | ACCTGC | 1 cut(s) | 542 |
| CciI | TCATGA | 1 cut(s) | 172 |
| Cfr13I | GGNCC | 3 cut(s) | 296, 326, 473 |
| Csp6I | GTAC | 2 cut(s) | 192, 418 |
| CviAII | CATG | 1 cut(s) | 173 |
| CviJI | RGCY | 3 cut(s) | 412, 475, 535 |
| CviKI_1 | RGCY | 3 cut(s) | 412, 475, 535 |
| CviQI | GTAC | 2 cut(s) | 192, 418 |
| DpnI | GATC | 2 cut(s) | 309, 543 |
| DpnII | GATC | 2 cut(s) | 307, 541 |
| Eam1104I | CTCTTC | 2 cut(s) | 96, 126 |
| EarI | CTCTTC | 2 cut(s) | 96, 126 |
| Eco47I | GGWCC | 2 cut(s) | 296, 326 |
| EcoO109I | RGGNCCY | 1 cut(s) | 296 |
| FaeI | CATG | 1 cut(s) | 176 |
| FaiI | YATR | 4 cut(s) | 174, 201, 303, 399 |
| FatI | CATG | 1 cut(s) | 172 |
| FbaI | TGATCA | 1 cut(s) | 541 |
| FspBI | CTAG | 1 cut(s) | 413 |
| GsuI | CTGGAG | 1 cut(s) | 315 |
| HaeIII | GGCC | 1 cut(s) | 475 |
| Hin1II | CATG | 1 cut(s) | 176 |
| Hpy166II | GTNNAC | 1 cut(s) | 418 |
| Hpy188III | TCNNGA | 3 cut(s) | 38, 173, 332 |
| Hpy8I | GTNNAC | 1 cut(s) | 418 |
| HpyAV | CCTTC | 2 cut(s) | 59, 553 |
| HpyCH4IV | ACGT | 1 cut(s) | 349 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 456, 551 |
| HpySE526I | ACGT | 1 cut(s) | 349 |
| Hsp92II | CATG | 1 cut(s) | 176 |
| Ksp22I | TGATCA | 1 cut(s) | 541 |
| Kzo9I | GATC | 2 cut(s) | 307, 541 |
| LguI | GCTCTTC | 2 cut(s) | 96, 126 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| LpnPI | CCDG | 4 cut(s) | 180, 237, 345, 537 |
| LweI | GCATC | 1 cut(s) | 208 |
| MaeI | CTAG | 1 cut(s) | 413 |
| MaeII | ACGT | 1 cut(s) | 349 |
| MaeIII | GTNAC | 1 cut(s) | 489 |
| MalI | GATC | 2 cut(s) | 309, 543 |
| MboI | GATC | 2 cut(s) | 307, 541 |
| MboII | GAAGA | 3 cut(s) | 113, 131, 143 |
| MfeI | CAATTG | 1 cut(s) | 523 |
| MflI | RGATCY | 1 cut(s) | 307 |
| MhlI | GDGCHC | 2 cut(s) | 108, 138 |
| MluCI | AATT | 7 cut(s) | 19, 59, 163, 247, 362, 523, 537 |
| MnlI | CCTC | 4 cut(s) | 309, 339, 462, 476 |
| MseI | TTAA | 5 cut(s) | 18, 26, 87, 380, 465 |
| MslI | CAYNNNNRTG | 1 cut(s) | 141 |
| MunI | CAATTG | 1 cut(s) | 523 |
| NdeII | GATC | 2 cut(s) | 307, 541 |
| NlaIII | CATG | 1 cut(s) | 176 |
| NlaIV | GGNNCC | 2 cut(s) | 297, 474 |
| PagI | TCATGA | 1 cut(s) | 172 |
| PciSI | GCTCTTC | 2 cut(s) | 96, 126 |
| PpuMI | RGGWCCY | 1 cut(s) | 296 |
| Psp5II | RGGWCCY | 1 cut(s) | 296 |
| PspN4I | GGNNCC | 2 cut(s) | 297, 474 |
| PspPI | GGNCC | 3 cut(s) | 296, 326, 473 |
| PspPPI | RGGWCCY | 1 cut(s) | 296 |
| PsuI | RGATCY | 1 cut(s) | 307 |
| RsaI | GTAC | 2 cut(s) | 193, 419 |
| RsaNI | GTAC | 2 cut(s) | 192, 418 |
| RseI | CAYNNNNRTG | 1 cut(s) | 141 |
| SapI | GCTCTTC | 2 cut(s) | 96, 126 |
| SaqAI | TTAA | 5 cut(s) | 18, 26, 87, 380, 465 |
| Sau3AI | GATC | 2 cut(s) | 307, 541 |
| Sau96I | GGNCC | 3 cut(s) | 296, 326, 473 |
| SduI | GDGCHC | 2 cut(s) | 108, 138 |
| SetI | ASST | 7 cut(s) | 47, 70, 352, 441, 487, 537, 556 |
| SfaNI | GCATC | 1 cut(s) | 208 |
| SinI | GGWCC | 2 cut(s) | 296, 326 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 141 |
| Sse9I | AATT | 7 cut(s) | 19, 59, 163, 247, 362, 523, 537 |
| SspI | AATATT | 1 cut(s) | 232 |
| SspMI | CTAG | 1 cut(s) | 413 |
| TaiI | ACGT | 1 cut(s) | 352 |
| TaqI | TCGA | 1 cut(s) | 159 |
| TasI | AATT | 7 cut(s) | 19, 59, 163, 247, 362, 523, 537 |
| TatI | WGTACW | 1 cut(s) | 417 |
| Tru1I | TTAA | 5 cut(s) | 18, 26, 87, 380, 465 |
| Tru9I | TTAA | 5 cut(s) | 18, 26, 87, 380, 465 |
| TscAI | CASTG | 2 cut(s) | 115, 145 |
| TspDTI | ATGAA | 3 cut(s) | 37, 105, 192 |
| TspRI | CASTG | 2 cut(s) | 115, 145 |
| VpaK11BI | GGWCC | 2 cut(s) | 296, 326 |
| XapI | RAATTY | 1 cut(s) | 362 |
| XspI | CTAG | 1 cut(s) | 413 |