Domain of unknown function (DUF4371)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1442, 1462 |
| AccI | GTMKAC | 1 cut(s) | 916 |
| AccII | CGCG | 1 cut(s) | 1525 |
| AccIII | TCCGGA | 1 cut(s) | 26 |
| AciI | CCGC | 1 cut(s) | 1935 |
| AclI | AACGTT | 1 cut(s) | 1978 |
| AclWI | GGATC | 4 cut(s) | 1236, 1613, 1626, 1874 |
| AcsI | RAATTY | 8 cut(s) | 446, 788, 936, 1390, 1707, 1905, 2023, 2185 |
| AcuI | CTGAAG | 2 cut(s) | 1800, 2250 |
| AdeI | CACNNNGTG | 1 cut(s) | 1347 |
| AfaI | GTAC | 5 cut(s) | 277, 503, 590, 1170, 1218 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 227, 566, 584, 851 |
| AflII | CTTAAG | 2 cut(s) | 620, 2137 |
| AflIII | ACRYGT | 2 cut(s) | 1523, 2115 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1270 |
| Alw21I | GWGCWC | 2 cut(s) | 162, 641 |
| Alw26I | GTCTC | 3 cut(s) | 1662, 1752, 2063 |
| AlwI | GGATC | 4 cut(s) | 1236, 1613, 1626, 1874 |
| Aor13HI | TCCGGA | 1 cut(s) | 26 |
| AoxI | GGCC | 1 cut(s) | 557 |
| ApeKI | GCWGC | 5 cut(s) | 979, 1204, 1741, 2051, 2208 |
| ApoI | RAATTY | 8 cut(s) | 446, 788, 936, 1390, 1707, 1905, 2023, 2185 |
| AseI | ATTAAT | 1 cut(s) | 654 |
| AspA2I | CCTAGG | 1 cut(s) | 1328 |
| AspS9I | GGNCC | 3 cut(s) | 380, 410, 557 |
| AsuHPI | GGTGA | 3 cut(s) | 596, 1070, 1705 |
| AvaII | GGWCC | 2 cut(s) | 380, 410 |
| AvrII | CCTAGG | 1 cut(s) | 1328 |
| BamHI | GGATCC | 1 cut(s) | 1618 |
| BanII | GRGCYC | 1 cut(s) | 222 |
| BbsI | GAAGAC | 1 cut(s) | 2118 |
| Bbv12I | GWGCWC | 2 cut(s) | 162, 641 |
| BbvI | GCAGC | 5 cut(s) | 966, 1191, 1728, 2038, 2195 |
| BccI | CCATC | 7 cut(s) | 161, 221, 578, 895, 1461, 2099, 2430 |
| BceAI | ACGGC | 1 cut(s) | 1556 |
| BclI | TGATCA | 2 cut(s) | 643, 2407 |
| BcoDI | GTCTC | 3 cut(s) | 1662, 1752, 2063 |
| BfaI | CTAG | 5 cut(s) | 108, 497, 869, 1329, 2172 |
| BfmI | CTRYAG | 3 cut(s) | 1205, 1742, 1773 |
| BfrI | CTTAAG | 2 cut(s) | 620, 2137 |
| BfuAI | ACCTGC | 2 cut(s) | 1442, 1462 |
| BglII | AGATCT | 1 cut(s) | 391 |
| BisI | GCNGC | 6 cut(s) | 980, 1205, 1742, 1936, 2052, 2209 |
| BlnI | CCTAGG | 1 cut(s) | 1328 |
| BlpI | GCTNAGC | 1 cut(s) | 1685 |
| BlsI | GCNGC | 6 cut(s) | 981, 1206, 1743, 1937, 2053, 2210 |
| Bme18I | GGWCC | 2 cut(s) | 380, 410 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1270 |
| BmgT120I | GGNCC | 3 cut(s) | 380, 410, 557 |
| BmiI | GGNNCC | 4 cut(s) | 114, 381, 558, 1620 |
| BmrI | ACTGGG | 1 cut(s) | 2207 |
| BmsI | GCATC | 4 cut(s) | 139, 292, 1021, 1825 |
| BmuI | ACTGGG | 1 cut(s) | 2207 |
| BpiI | GAAGAC | 1 cut(s) | 2118 |
| BpmI | CTGGAG | 2 cut(s) | 399, 772 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1685 |
| BpuEI | CTTGAG | 2 cut(s) | 490, 2087 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1323, 1878 |
| BsaJI | CCNNGG | 3 cut(s) | 817, 1294, 1328 |
| BsaWI | WCCGGW | 1 cut(s) | 26 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 227, 566, 584, 851 |
| Bse1I | ACTGG | 3 cut(s) | 755, 2199, 2213 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1323, 1878 |
| BseAI | TCCGGA | 1 cut(s) | 26 |
| BseDI | CCNNGG | 3 cut(s) | 817, 1294, 1328 |
| BseJI | GATNNNNATC | 2 cut(s) | 1323, 1878 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 227, 566, 584, 851 |
| BseMII | CTCAG | 1 cut(s) | 2262 |
| BseNI | ACTGG | 3 cut(s) | 755, 2199, 2213 |
| BseRI | GAGGAG | 2 cut(s) | 920, 2290 |
| BseXI | GCAGC | 5 cut(s) | 966, 1191, 1728, 2038, 2195 |
| Bsh1236I | CGCG | 1 cut(s) | 1525 |
| BshFI | GGCC | 1 cut(s) | 559 |
| BsiHKAI | GWGCWC | 2 cut(s) | 162, 641 |
| BsiSI | CCGG | 1 cut(s) | 27 |
| BslFI | GGGAC | 2 cut(s) | 1203, 1266 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 227, 566, 584, 851 |
| BsmAI | GTCTC | 3 cut(s) | 1662, 1752, 2063 |
| BsmFI | GGGAC | 2 cut(s) | 1203, 1266 |
| BsmI | GAATGC | 1 cut(s) | 1120 |
| BsnI | GGCC | 1 cut(s) | 559 |
| Bsp1286I | GDGCHC | 3 cut(s) | 162, 222, 641 |
| Bsp13I | TCCGGA | 1 cut(s) | 26 |
| Bsp1407I | TGTACA | 1 cut(s) | 1168 |
| Bsp143I | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1685 |
| BspACI | CCGC | 1 cut(s) | 1935 |
| BspANI | GGCC | 1 cut(s) | 559 |
| BspCNI | CTCAG | 1 cut(s) | 2263 |
| BspEI | TCCGGA | 1 cut(s) | 26 |
| BspFNI | CGCG | 1 cut(s) | 1525 |
| BspHI | TCATGA | 2 cut(s) | 256, 1750 |
| BspLI | GGNNCC | 4 cut(s) | 114, 381, 558, 1620 |
| BspMAI | CTGCAG | 2 cut(s) | 1209, 1746 |
| BspMI | ACCTGC | 2 cut(s) | 1442, 1462 |
| BspPI | GGATC | 4 cut(s) | 1236, 1613, 1626, 1874 |
| BspQI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1194 |
| BspTI | CTTAAG | 2 cut(s) | 620, 2137 |
| BsrGI | TGTACA | 1 cut(s) | 1168 |
| BsrI | ACTGG | 3 cut(s) | 755, 2199, 2213 |
| BssECI | CCNNGG | 3 cut(s) | 817, 1294, 1328 |
| BssMI | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| BssT1I | CCWWGG | 2 cut(s) | 1294, 1328 |
| Bst4CI | ACNGT | 3 cut(s) | 2074, 2248, 2260 |
| Bst6I | CTCTTC | 5 cut(s) | 150, 180, 210, 1137, 1194 |
| BstAFI | CTTAAG | 2 cut(s) | 620, 2137 |
| BstAUI | TGTACA | 1 cut(s) | 1168 |
| BstC8I | GCNNGC | 1 cut(s) | 1209 |
| BstDEI | CTNAG | 8 cut(s) | 386, 608, 683, 808, 1103, 1685, 1941, 2271 |
| BstDSI | CCRYGG | 1 cut(s) | 817 |
| BstEII | GGTNACC | 1 cut(s) | 602 |
| BstFNI | CGCG | 1 cut(s) | 1525 |
| BstKTI | GATC | 8 cut(s) | 394, 646, 1231, 1621, 1663, 1882, 2318, 2410 |
| BstMAI | GTCTC | 3 cut(s) | 1662, 1752, 2063 |
| BstMBI | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| BstNSI | RCATGY | 1 cut(s) | 2119 |
| BstPI | GGTNACC | 1 cut(s) | 602 |
| BstSFI | CTRYAG | 3 cut(s) | 1205, 1742, 1773 |
| BstUI | CGCG | 1 cut(s) | 1525 |
| BstV1I | GCAGC | 5 cut(s) | 966, 1191, 1728, 2038, 2195 |
| BstV2I | GAAGAC | 1 cut(s) | 2118 |
| BstX2I | RGATCY | 3 cut(s) | 391, 1228, 1618 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 333 |
| BstYI | RGATCY | 3 cut(s) | 391, 1228, 1618 |
| BsuRI | GGCC | 1 cut(s) | 559 |
| BtgI | CCRYGG | 1 cut(s) | 817 |
| BtgZI | GCGATG | 1 cut(s) | 1480 |
| BtsI | GCAGTG | 1 cut(s) | 1413 |
| BtsIMutI | CAGTG | 5 cut(s) | 162, 222, 639, 748, 1413 |
| BveI | ACCTGC | 2 cut(s) | 1442, 1462 |
| Cac8I | GCNNGC | 1 cut(s) | 1209 |
| CciI | TCATGA | 2 cut(s) | 256, 1750 |
| Cfr13I | GGNCC | 3 cut(s) | 380, 410, 557 |
| CseI | GACGC | 3 cut(s) | 668, 927, 1889 |
| Csp6I | GTAC | 5 cut(s) | 276, 502, 589, 1169, 1217 |
| CviAII | CATG | 7 cut(s) | 257, 629, 698, 1172, 1690, 1751, 2116 |
| CviQI | GTAC | 5 cut(s) | 276, 502, 589, 1169, 1217 |
| DdeI | CTNAG | 8 cut(s) | 386, 608, 683, 808, 1103, 1685, 1941, 2271 |
| DpnI | GATC | 8 cut(s) | 393, 645, 1230, 1620, 1662, 1881, 2317, 2409 |
| DpnII | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| DraI | TTTAAA | 2 cut(s) | 1164, 1365 |
| DraIII | CACNNNGTG | 1 cut(s) | 1347 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1270 |
| Eam1104I | CTCTTC | 5 cut(s) | 150, 180, 210, 1137, 1194 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1270 |
| EarI | CTCTTC | 5 cut(s) | 150, 180, 210, 1137, 1194 |
| Eco130I | CCWWGG | 2 cut(s) | 1294, 1328 |
| Eco24I | GRGCYC | 1 cut(s) | 222 |
| Eco32I | GATATC | 2 cut(s) | 1321, 1990 |
| Eco47I | GGWCC | 2 cut(s) | 380, 410 |
| Eco57I | CTGAAG | 2 cut(s) | 1800, 2250 |
| Eco91I | GGTNACC | 1 cut(s) | 602 |
| EcoO109I | RGGNCCY | 1 cut(s) | 380 |
| EcoO65I | GGTNACC | 1 cut(s) | 602 |
| EcoRI | GAATTC | 1 cut(s) | 2023 |
| EcoRV | GATATC | 2 cut(s) | 1321, 1990 |
| EcoT14I | CCWWGG | 2 cut(s) | 1294, 1328 |
| EcoT38I | GRGCYC | 1 cut(s) | 222 |
| ErhI | CCWWGG | 2 cut(s) | 1294, 1328 |
| FaeI | CATG | 7 cut(s) | 260, 632, 701, 1175, 1693, 1754, 2119 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1578, 1610 |
| FaqI | GGGAC | 2 cut(s) | 1203, 1266 |
| FatI | CATG | 7 cut(s) | 256, 628, 697, 1171, 1689, 1750, 2115 |
| FauNDI | CATATG | 1 cut(s) | 984 |
| FbaI | TGATCA | 2 cut(s) | 643, 2407 |
| FblI | GTMKAC | 1 cut(s) | 916 |
| Fnu4HI | GCNGC | 6 cut(s) | 980, 1205, 1742, 1936, 2052, 2209 |
| FriOI | GRGCYC | 1 cut(s) | 222 |
| Fsp4HI | GCNGC | 6 cut(s) | 980, 1205, 1742, 1936, 2052, 2209 |
| FspBI | CTAG | 5 cut(s) | 108, 497, 869, 1329, 2172 |
| GluI | GCNGC | 6 cut(s) | 980, 1205, 1742, 1936, 2052, 2209 |
| GsuI | CTGGAG | 2 cut(s) | 399, 772 |
| HaeIII | GGCC | 1 cut(s) | 559 |
| HapII | CCGG | 1 cut(s) | 27 |
| HgaI | GACGC | 3 cut(s) | 668, 927, 1889 |
| Hin1II | CATG | 7 cut(s) | 260, 632, 701, 1175, 1693, 1754, 2119 |
| HincII | GTYRAC | 2 cut(s) | 73, 2244 |
| HindII | GTYRAC | 2 cut(s) | 73, 2244 |
| HindIII | AAGCTT | 1 cut(s) | 714 |
| HinfI | GANTC | 4 cut(s) | 1126, 1497, 1534, 1563 |
| HpaI | GTTAAC | 2 cut(s) | 73, 2244 |
| HpaII | CCGG | 1 cut(s) | 27 |
| HphI | GGTGA | 3 cut(s) | 596, 1070, 1705 |
| Hpy166II | GTNNAC | 5 cut(s) | 73, 131, 502, 917, 2244 |
| Hpy188I | TCNGA | 4 cut(s) | 934, 969, 2014, 2098 |
| Hpy8I | GTNNAC | 5 cut(s) | 73, 131, 502, 917, 2244 |
| HpyAV | CCTTC | 5 cut(s) | 113, 370, 1474, 1953, 2419 |
| HpyCH4III | ACNGT | 3 cut(s) | 2074, 2248, 2260 |
| HpyCH4IV | ACGT | 3 cut(s) | 433, 1432, 1978 |
| HpyF3I | CTNAG | 8 cut(s) | 386, 608, 683, 808, 1103, 1685, 1941, 2271 |
| HpySE526I | ACGT | 3 cut(s) | 433, 1432, 1978 |
| Hsp92II | CATG | 7 cut(s) | 260, 632, 701, 1175, 1693, 1754, 2119 |
| Kpn2I | TCCGGA | 1 cut(s) | 26 |
| Ksp22I | TGATCA | 2 cut(s) | 643, 2407 |
| KspAI | GTTAAC | 2 cut(s) | 73, 2244 |
| Kzo9I | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| LguI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1194 |
| LmnI | GCTCC | 3 cut(s) | 511, 625, 907 |
| Lsp1109I | GCAGC | 5 cut(s) | 966, 1191, 1728, 2038, 2195 |
| LweI | GCATC | 4 cut(s) | 139, 292, 1021, 1825 |
| MaeI | CTAG | 5 cut(s) | 108, 497, 869, 1329, 2172 |
| MaeII | ACGT | 3 cut(s) | 433, 1432, 1978 |
| MaeIII | GTNAC | 5 cut(s) | 573, 602, 1076, 2254, 2325 |
| MalI | GATC | 8 cut(s) | 393, 645, 1230, 1620, 1662, 1881, 2317, 2409 |
| MboI | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| MfeI | CAATTG | 3 cut(s) | 780, 1598, 2389 |
| MflI | RGATCY | 3 cut(s) | 391, 1228, 1618 |
| MhlI | GDGCHC | 3 cut(s) | 162, 222, 641 |
| MluI | ACGCGT | 1 cut(s) | 1523 |
| MlyI | GAGTC | 2 cut(s) | 1135, 1543 |
| MroI | TCCGGA | 1 cut(s) | 26 |
| MslI | CAYNNNNRTG | 1 cut(s) | 165 |
| MspA1I | CMGCKG | 1 cut(s) | 2270 |
| MspCI | CTTAAG | 2 cut(s) | 620, 2137 |
| MspI | CCGG | 1 cut(s) | 27 |
| MssI | GTTTAAAC | 1 cut(s) | 1365 |
| MunI | CAATTG | 3 cut(s) | 780, 1598, 2389 |
| Mva1269I | GAATGC | 1 cut(s) | 1120 |
| MvnI | CGCG | 1 cut(s) | 1525 |
| NdeI | CATATG | 1 cut(s) | 984 |
| NdeII | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| NlaIII | CATG | 7 cut(s) | 260, 632, 701, 1175, 1693, 1754, 2119 |
| NlaIV | GGNNCC | 4 cut(s) | 114, 381, 558, 1620 |
| NmuCI | GTSAC | 3 cut(s) | 602, 1076, 2325 |
| NspI | RCATGY | 1 cut(s) | 2119 |
| PagI | TCATGA | 2 cut(s) | 256, 1750 |
| PciI | ACATGT | 1 cut(s) | 2115 |
| PciSI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1194 |
| PctI | GAATGC | 1 cut(s) | 1120 |
| PfeI | GAWTC | 2 cut(s) | 1497, 1563 |
| PkrI | GCNGC | 6 cut(s) | 981, 1206, 1743, 1937, 2053, 2210 |
| PleI | GAGTC | 2 cut(s) | 1134, 1542 |
| PmeI | GTTTAAAC | 1 cut(s) | 1365 |
| PpsI | GAGTC | 2 cut(s) | 1134, 1542 |
| PpuMI | RGGWCCY | 1 cut(s) | 380 |
| PscI | ACATGT | 1 cut(s) | 2115 |
| PshBI | ATTAAT | 1 cut(s) | 654 |
| Psp1406I | AACGTT | 1 cut(s) | 1978 |
| Psp5II | RGGWCCY | 1 cut(s) | 380 |
| PspEI | GGTNACC | 1 cut(s) | 602 |
| PspN4I | GGNNCC | 4 cut(s) | 114, 381, 558, 1620 |
| PspPI | GGNCC | 3 cut(s) | 380, 410, 557 |
| PspPPI | RGGWCCY | 1 cut(s) | 380 |
| PstI | CTGCAG | 2 cut(s) | 1209, 1746 |
| PsuI | RGATCY | 3 cut(s) | 391, 1228, 1618 |
| PvuII | CAGCTG | 1 cut(s) | 2270 |
| RsaI | GTAC | 5 cut(s) | 277, 503, 590, 1170, 1218 |
| RsaNI | GTAC | 5 cut(s) | 276, 502, 589, 1169, 1217 |
| RseI | CAYNNNNRTG | 1 cut(s) | 165 |
| SapI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1194 |
| SatI | GCNGC | 6 cut(s) | 980, 1205, 1742, 1936, 2052, 2209 |
| Sau3AI | GATC | 8 cut(s) | 391, 643, 1228, 1618, 1660, 1879, 2315, 2407 |
| Sau96I | GGNCC | 3 cut(s) | 380, 410, 557 |
| SchI | GAGTC | 2 cut(s) | 1135, 1543 |
| SduI | GDGCHC | 3 cut(s) | 162, 222, 641 |
| SfaNI | GCATC | 4 cut(s) | 139, 292, 1021, 1825 |
| SfcI | CTRYAG | 3 cut(s) | 1205, 1742, 1773 |
| SinI | GGWCC | 2 cut(s) | 380, 410 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 165 |
| SmlI | CTYRAG | 4 cut(s) | 505, 620, 2066, 2137 |
| SmoI | CTYRAG | 4 cut(s) | 505, 620, 2066, 2137 |
| SsiI | CCGC | 1 cut(s) | 1935 |
| SspI | AATATT | 5 cut(s) | 20, 316, 1040, 1425, 2238 |
| SspMI | CTAG | 5 cut(s) | 108, 497, 869, 1329, 2172 |
| StyI | CCWWGG | 2 cut(s) | 1294, 1328 |
| TaaI | ACNGT | 3 cut(s) | 2074, 2248, 2260 |
| TaiI | ACGT | 3 cut(s) | 436, 1435, 1981 |
| TaqI | TCGA | 2 cut(s) | 2233, 2360 |
| TatI | WGTACW | 2 cut(s) | 501, 1168 |
| TauI | GCSGC | 1 cut(s) | 1938 |
| TfiI | GAWTC | 2 cut(s) | 1497, 1563 |
| TscAI | CASTG | 5 cut(s) | 169, 229, 646, 755, 1413 |
| TseFI | GTSAC | 3 cut(s) | 602, 1076, 2325 |
| TseI | GCWGC | 5 cut(s) | 979, 1204, 1741, 2051, 2208 |
| Tsp45I | GTSAC | 3 cut(s) | 602, 1076, 2325 |
| TspGWI | ACGGA | 2 cut(s) | 806, 1335 |
| TspRI | CASTG | 5 cut(s) | 169, 229, 646, 755, 1413 |
| Vha464I | CTTAAG | 2 cut(s) | 620, 2137 |
| VpaK11BI | GGWCC | 2 cut(s) | 380, 410 |
| VspI | ATTAAT | 1 cut(s) | 654 |
| XapI | RAATTY | 8 cut(s) | 446, 788, 936, 1390, 1707, 1905, 2023, 2185 |
| XceI | RCATGY | 1 cut(s) | 2119 |
| XmaJI | CCTAGG | 1 cut(s) | 1328 |
| XmiI | GTMKAC | 1 cut(s) | 916 |
| XspI | CTAG | 5 cut(s) | 108, 497, 869, 1329, 2172 |