Domain of unknown function (DUF4371)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1325, 1345 |
| AccI | GTMKAC | 1 cut(s) | 799 |
| AccII | CGCG | 1 cut(s) | 1408 |
| AccIII | TCCGGA | 1 cut(s) | 26 |
| AciI | CCGC | 1 cut(s) | 1818 |
| AclI | AACGTT | 1 cut(s) | 1861 |
| AclWI | GGATC | 4 cut(s) | 1119, 1496, 1509, 1757 |
| AcsI | RAATTY | 8 cut(s) | 446, 671, 819, 1273, 1590, 1788, 1906, 2068 |
| AcuI | CTGAAG | 2 cut(s) | 1683, 2133 |
| AdeI | CACNNNGTG | 1 cut(s) | 1230 |
| AfaI | GTAC | 3 cut(s) | 277, 1053, 1101 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 227, 734 |
| AflII | CTTAAG | 2 cut(s) | 503, 2020 |
| AflIII | ACRYGT | 2 cut(s) | 1406, 1998 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1153 |
| Alw21I | GWGCWC | 2 cut(s) | 162, 524 |
| Alw26I | GTCTC | 3 cut(s) | 1545, 1635, 1946 |
| AlwI | GGATC | 4 cut(s) | 1119, 1496, 1509, 1757 |
| Aor13HI | TCCGGA | 1 cut(s) | 26 |
| ApeKI | GCWGC | 5 cut(s) | 862, 1087, 1624, 1934, 2091 |
| ApoI | RAATTY | 8 cut(s) | 446, 671, 819, 1273, 1590, 1788, 1906, 2068 |
| AseI | ATTAAT | 1 cut(s) | 537 |
| AspA2I | CCTAGG | 1 cut(s) | 1211 |
| AspS9I | GGNCC | 2 cut(s) | 380, 410 |
| AsuHPI | GGTGA | 2 cut(s) | 953, 1588 |
| AvaII | GGWCC | 2 cut(s) | 380, 410 |
| AvrII | CCTAGG | 1 cut(s) | 1211 |
| BamHI | GGATCC | 1 cut(s) | 1501 |
| BanII | GRGCYC | 1 cut(s) | 222 |
| BbsI | GAAGAC | 1 cut(s) | 2001 |
| Bbv12I | GWGCWC | 2 cut(s) | 162, 524 |
| BbvI | GCAGC | 5 cut(s) | 849, 1074, 1611, 1921, 2078 |
| BccI | CCATC | 6 cut(s) | 161, 221, 778, 1344, 1982, 2313 |
| BceAI | ACGGC | 1 cut(s) | 1439 |
| BclI | TGATCA | 2 cut(s) | 526, 2290 |
| BcoDI | GTCTC | 3 cut(s) | 1545, 1635, 1946 |
| BfaI | CTAG | 4 cut(s) | 108, 752, 1212, 2055 |
| BfmI | CTRYAG | 3 cut(s) | 1088, 1625, 1656 |
| BfrI | CTTAAG | 2 cut(s) | 503, 2020 |
| BfuAI | ACCTGC | 2 cut(s) | 1325, 1345 |
| BglII | AGATCT | 1 cut(s) | 391 |
| BisI | GCNGC | 6 cut(s) | 863, 1088, 1625, 1819, 1935, 2092 |
| BlnI | CCTAGG | 1 cut(s) | 1211 |
| BlpI | GCTNAGC | 1 cut(s) | 1568 |
| BlsI | GCNGC | 6 cut(s) | 864, 1089, 1626, 1820, 1936, 2093 |
| Bme18I | GGWCC | 2 cut(s) | 380, 410 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1153 |
| BmgT120I | GGNCC | 2 cut(s) | 380, 410 |
| BmiI | GGNNCC | 3 cut(s) | 114, 381, 1503 |
| BmrI | ACTGGG | 1 cut(s) | 2090 |
| BmsI | GCATC | 4 cut(s) | 139, 292, 904, 1708 |
| BmuI | ACTGGG | 1 cut(s) | 2090 |
| BpiI | GAAGAC | 1 cut(s) | 2001 |
| BpmI | CTGGAG | 2 cut(s) | 399, 655 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1568 |
| BpuEI | CTTGAG | 1 cut(s) | 1970 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1206, 1761 |
| BsaJI | CCNNGG | 3 cut(s) | 700, 1177, 1211 |
| BsaWI | WCCGGW | 1 cut(s) | 26 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 227, 734 |
| Bse1I | ACTGG | 3 cut(s) | 638, 2082, 2096 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1206, 1761 |
| BseAI | TCCGGA | 1 cut(s) | 26 |
| BseDI | CCNNGG | 3 cut(s) | 700, 1177, 1211 |
| BseJI | GATNNNNATC | 2 cut(s) | 1206, 1761 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 227, 734 |
| BseMII | CTCAG | 1 cut(s) | 2145 |
| BseNI | ACTGG | 3 cut(s) | 638, 2082, 2096 |
| BseRI | GAGGAG | 2 cut(s) | 803, 2173 |
| BseXI | GCAGC | 5 cut(s) | 849, 1074, 1611, 1921, 2078 |
| Bsh1236I | CGCG | 1 cut(s) | 1408 |
| BsiHKAI | GWGCWC | 2 cut(s) | 162, 524 |
| BsiSI | CCGG | 1 cut(s) | 27 |
| BslFI | GGGAC | 2 cut(s) | 1086, 1149 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 227, 734 |
| BsmAI | GTCTC | 3 cut(s) | 1545, 1635, 1946 |
| BsmFI | GGGAC | 2 cut(s) | 1086, 1149 |
| BsmI | GAATGC | 1 cut(s) | 1003 |
| Bsp1286I | GDGCHC | 3 cut(s) | 162, 222, 524 |
| Bsp13I | TCCGGA | 1 cut(s) | 26 |
| Bsp1407I | TGTACA | 1 cut(s) | 1051 |
| Bsp143I | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1568 |
| BspACI | CCGC | 1 cut(s) | 1818 |
| BspCNI | CTCAG | 1 cut(s) | 2146 |
| BspEI | TCCGGA | 1 cut(s) | 26 |
| BspFNI | CGCG | 1 cut(s) | 1408 |
| BspHI | TCATGA | 2 cut(s) | 256, 1633 |
| BspLI | GGNNCC | 3 cut(s) | 114, 381, 1503 |
| BspMAI | CTGCAG | 2 cut(s) | 1092, 1629 |
| BspMI | ACCTGC | 2 cut(s) | 1325, 1345 |
| BspPI | GGATC | 4 cut(s) | 1119, 1496, 1509, 1757 |
| BspQI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1077 |
| BspTI | CTTAAG | 2 cut(s) | 503, 2020 |
| BsrGI | TGTACA | 1 cut(s) | 1051 |
| BsrI | ACTGG | 3 cut(s) | 638, 2082, 2096 |
| BssECI | CCNNGG | 3 cut(s) | 700, 1177, 1211 |
| BssMI | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| BssT1I | CCWWGG | 2 cut(s) | 1177, 1211 |
| Bst4CI | ACNGT | 3 cut(s) | 1957, 2131, 2143 |
| Bst6I | CTCTTC | 5 cut(s) | 150, 180, 210, 1020, 1077 |
| BstAFI | CTTAAG | 2 cut(s) | 503, 2020 |
| BstAUI | TGTACA | 1 cut(s) | 1051 |
| BstC8I | GCNNGC | 1 cut(s) | 1092 |
| BstDEI | CTNAG | 7 cut(s) | 386, 566, 691, 986, 1568, 1824, 2154 |
| BstDSI | CCRYGG | 1 cut(s) | 700 |
| BstFNI | CGCG | 1 cut(s) | 1408 |
| BstKTI | GATC | 8 cut(s) | 394, 529, 1114, 1504, 1546, 1765, 2201, 2293 |
| BstMAI | GTCTC | 3 cut(s) | 1545, 1635, 1946 |
| BstMBI | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| BstNSI | RCATGY | 1 cut(s) | 2002 |
| BstSFI | CTRYAG | 3 cut(s) | 1088, 1625, 1656 |
| BstUI | CGCG | 1 cut(s) | 1408 |
| BstV1I | GCAGC | 5 cut(s) | 849, 1074, 1611, 1921, 2078 |
| BstV2I | GAAGAC | 1 cut(s) | 2001 |
| BstX2I | RGATCY | 3 cut(s) | 391, 1111, 1501 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 333 |
| BstYI | RGATCY | 3 cut(s) | 391, 1111, 1501 |
| BtgI | CCRYGG | 1 cut(s) | 700 |
| BtgZI | GCGATG | 1 cut(s) | 1363 |
| BtsI | GCAGTG | 1 cut(s) | 1296 |
| BtsIMutI | CAGTG | 5 cut(s) | 162, 222, 522, 631, 1296 |
| BveI | ACCTGC | 2 cut(s) | 1325, 1345 |
| Cac8I | GCNNGC | 1 cut(s) | 1092 |
| CciI | TCATGA | 2 cut(s) | 256, 1633 |
| Cfr13I | GGNCC | 2 cut(s) | 380, 410 |
| CseI | GACGC | 3 cut(s) | 551, 810, 1772 |
| Csp6I | GTAC | 3 cut(s) | 276, 1052, 1100 |
| CviAII | CATG | 7 cut(s) | 257, 512, 581, 1055, 1573, 1634, 1999 |
| CviQI | GTAC | 3 cut(s) | 276, 1052, 1100 |
| DdeI | CTNAG | 7 cut(s) | 386, 566, 691, 986, 1568, 1824, 2154 |
| DpnI | GATC | 8 cut(s) | 393, 528, 1113, 1503, 1545, 1764, 2200, 2292 |
| DpnII | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| DraI | TTTAAA | 2 cut(s) | 1047, 1248 |
| DraIII | CACNNNGTG | 1 cut(s) | 1230 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1153 |
| Eam1104I | CTCTTC | 5 cut(s) | 150, 180, 210, 1020, 1077 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1153 |
| EarI | CTCTTC | 5 cut(s) | 150, 180, 210, 1020, 1077 |
| Eco130I | CCWWGG | 2 cut(s) | 1177, 1211 |
| Eco24I | GRGCYC | 1 cut(s) | 222 |
| Eco32I | GATATC | 2 cut(s) | 1204, 1873 |
| Eco47I | GGWCC | 2 cut(s) | 380, 410 |
| Eco57I | CTGAAG | 2 cut(s) | 1683, 2133 |
| EcoO109I | RGGNCCY | 1 cut(s) | 380 |
| EcoRI | GAATTC | 1 cut(s) | 1906 |
| EcoRV | GATATC | 2 cut(s) | 1204, 1873 |
| EcoT14I | CCWWGG | 2 cut(s) | 1177, 1211 |
| EcoT38I | GRGCYC | 1 cut(s) | 222 |
| ErhI | CCWWGG | 2 cut(s) | 1177, 1211 |
| FaeI | CATG | 7 cut(s) | 260, 515, 584, 1058, 1576, 1637, 2002 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1461, 1493 |
| FaqI | GGGAC | 2 cut(s) | 1086, 1149 |
| FatI | CATG | 7 cut(s) | 256, 511, 580, 1054, 1572, 1633, 1998 |
| FauNDI | CATATG | 1 cut(s) | 867 |
| FbaI | TGATCA | 2 cut(s) | 526, 2290 |
| FblI | GTMKAC | 1 cut(s) | 799 |
| Fnu4HI | GCNGC | 6 cut(s) | 863, 1088, 1625, 1819, 1935, 2092 |
| FriOI | GRGCYC | 1 cut(s) | 222 |
| Fsp4HI | GCNGC | 6 cut(s) | 863, 1088, 1625, 1819, 1935, 2092 |
| FspBI | CTAG | 4 cut(s) | 108, 752, 1212, 2055 |
| GluI | GCNGC | 6 cut(s) | 863, 1088, 1625, 1819, 1935, 2092 |
| GsuI | CTGGAG | 2 cut(s) | 399, 655 |
| HapII | CCGG | 1 cut(s) | 27 |
| HgaI | GACGC | 3 cut(s) | 551, 810, 1772 |
| Hin1II | CATG | 7 cut(s) | 260, 515, 584, 1058, 1576, 1637, 2002 |
| HincII | GTYRAC | 2 cut(s) | 73, 2127 |
| HindII | GTYRAC | 2 cut(s) | 73, 2127 |
| HindIII | AAGCTT | 1 cut(s) | 597 |
| HinfI | GANTC | 4 cut(s) | 1009, 1380, 1417, 1446 |
| HpaI | GTTAAC | 2 cut(s) | 73, 2127 |
| HpaII | CCGG | 1 cut(s) | 27 |
| HphI | GGTGA | 2 cut(s) | 953, 1588 |
| Hpy166II | GTNNAC | 4 cut(s) | 73, 131, 800, 2127 |
| Hpy188I | TCNGA | 4 cut(s) | 817, 852, 1897, 1981 |
| Hpy8I | GTNNAC | 4 cut(s) | 73, 131, 800, 2127 |
| HpyAV | CCTTC | 5 cut(s) | 113, 370, 1357, 1836, 2302 |
| HpyCH4III | ACNGT | 3 cut(s) | 1957, 2131, 2143 |
| HpyCH4IV | ACGT | 3 cut(s) | 433, 1315, 1861 |
| HpyF3I | CTNAG | 7 cut(s) | 386, 566, 691, 986, 1568, 1824, 2154 |
| HpySE526I | ACGT | 3 cut(s) | 433, 1315, 1861 |
| Hsp92II | CATG | 7 cut(s) | 260, 515, 584, 1058, 1576, 1637, 2002 |
| Kpn2I | TCCGGA | 1 cut(s) | 26 |
| Ksp22I | TGATCA | 2 cut(s) | 526, 2290 |
| KspAI | GTTAAC | 2 cut(s) | 73, 2127 |
| Kzo9I | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| LguI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1077 |
| LmnI | GCTCC | 2 cut(s) | 508, 790 |
| Lsp1109I | GCAGC | 5 cut(s) | 849, 1074, 1611, 1921, 2078 |
| LweI | GCATC | 4 cut(s) | 139, 292, 904, 1708 |
| MaeI | CTAG | 4 cut(s) | 108, 752, 1212, 2055 |
| MaeII | ACGT | 3 cut(s) | 433, 1315, 1861 |
| MaeIII | GTNAC | 3 cut(s) | 959, 2137, 2208 |
| MalI | GATC | 8 cut(s) | 393, 528, 1113, 1503, 1545, 1764, 2200, 2292 |
| MboI | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| MfeI | CAATTG | 3 cut(s) | 663, 1481, 2272 |
| MflI | RGATCY | 3 cut(s) | 391, 1111, 1501 |
| MhlI | GDGCHC | 3 cut(s) | 162, 222, 524 |
| MluI | ACGCGT | 1 cut(s) | 1406 |
| MlyI | GAGTC | 2 cut(s) | 1018, 1426 |
| MroI | TCCGGA | 1 cut(s) | 26 |
| MslI | CAYNNNNRTG | 1 cut(s) | 165 |
| MspA1I | CMGCKG | 1 cut(s) | 2153 |
| MspCI | CTTAAG | 2 cut(s) | 503, 2020 |
| MspI | CCGG | 1 cut(s) | 27 |
| MssI | GTTTAAAC | 1 cut(s) | 1248 |
| MunI | CAATTG | 3 cut(s) | 663, 1481, 2272 |
| Mva1269I | GAATGC | 1 cut(s) | 1003 |
| MvnI | CGCG | 1 cut(s) | 1408 |
| NdeI | CATATG | 1 cut(s) | 867 |
| NdeII | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| NlaIII | CATG | 7 cut(s) | 260, 515, 584, 1058, 1576, 1637, 2002 |
| NlaIV | GGNNCC | 3 cut(s) | 114, 381, 1503 |
| NmuCI | GTSAC | 2 cut(s) | 959, 2208 |
| NspI | RCATGY | 1 cut(s) | 2002 |
| PagI | TCATGA | 2 cut(s) | 256, 1633 |
| PciI | ACATGT | 1 cut(s) | 1998 |
| PciSI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1077 |
| PctI | GAATGC | 1 cut(s) | 1003 |
| PfeI | GAWTC | 2 cut(s) | 1380, 1446 |
| PkrI | GCNGC | 6 cut(s) | 864, 1089, 1626, 1820, 1936, 2093 |
| PleI | GAGTC | 2 cut(s) | 1017, 1425 |
| PmeI | GTTTAAAC | 1 cut(s) | 1248 |
| PpsI | GAGTC | 2 cut(s) | 1017, 1425 |
| PpuMI | RGGWCCY | 1 cut(s) | 380 |
| PscI | ACATGT | 1 cut(s) | 1998 |
| PshBI | ATTAAT | 1 cut(s) | 537 |
| Psp1406I | AACGTT | 1 cut(s) | 1861 |
| Psp5II | RGGWCCY | 1 cut(s) | 380 |
| PspN4I | GGNNCC | 3 cut(s) | 114, 381, 1503 |
| PspPI | GGNCC | 2 cut(s) | 380, 410 |
| PspPPI | RGGWCCY | 1 cut(s) | 380 |
| PstI | CTGCAG | 2 cut(s) | 1092, 1629 |
| PsuI | RGATCY | 3 cut(s) | 391, 1111, 1501 |
| PvuII | CAGCTG | 1 cut(s) | 2153 |
| RsaI | GTAC | 3 cut(s) | 277, 1053, 1101 |
| RsaNI | GTAC | 3 cut(s) | 276, 1052, 1100 |
| RseI | CAYNNNNRTG | 1 cut(s) | 165 |
| SapI | GCTCTTC | 4 cut(s) | 150, 180, 210, 1077 |
| SatI | GCNGC | 6 cut(s) | 863, 1088, 1625, 1819, 1935, 2092 |
| Sau3AI | GATC | 8 cut(s) | 391, 526, 1111, 1501, 1543, 1762, 2198, 2290 |
| Sau96I | GGNCC | 2 cut(s) | 380, 410 |
| SchI | GAGTC | 2 cut(s) | 1018, 1426 |
| SduI | GDGCHC | 3 cut(s) | 162, 222, 524 |
| SfaNI | GCATC | 4 cut(s) | 139, 292, 904, 1708 |
| SfcI | CTRYAG | 3 cut(s) | 1088, 1625, 1656 |
| SinI | GGWCC | 2 cut(s) | 380, 410 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 165 |
| SmlI | CTYRAG | 3 cut(s) | 503, 1949, 2020 |
| SmoI | CTYRAG | 3 cut(s) | 503, 1949, 2020 |
| SsiI | CCGC | 1 cut(s) | 1818 |
| SspI | AATATT | 5 cut(s) | 20, 316, 923, 1308, 2121 |
| SspMI | CTAG | 4 cut(s) | 108, 752, 1212, 2055 |
| StyI | CCWWGG | 2 cut(s) | 1177, 1211 |
| TaaI | ACNGT | 3 cut(s) | 1957, 2131, 2143 |
| TaiI | ACGT | 3 cut(s) | 436, 1318, 1864 |
| TaqI | TCGA | 2 cut(s) | 2116, 2243 |
| TatI | WGTACW | 1 cut(s) | 1051 |
| TauI | GCSGC | 1 cut(s) | 1821 |
| TfiI | GAWTC | 2 cut(s) | 1380, 1446 |
| TscAI | CASTG | 5 cut(s) | 169, 229, 529, 638, 1296 |
| TseFI | GTSAC | 2 cut(s) | 959, 2208 |
| TseI | GCWGC | 5 cut(s) | 862, 1087, 1624, 1934, 2091 |
| Tsp45I | GTSAC | 2 cut(s) | 959, 2208 |
| TspGWI | ACGGA | 2 cut(s) | 689, 1218 |
| TspRI | CASTG | 5 cut(s) | 169, 229, 529, 638, 1296 |
| Vha464I | CTTAAG | 2 cut(s) | 503, 2020 |
| VpaK11BI | GGWCC | 2 cut(s) | 380, 410 |
| VspI | ATTAAT | 1 cut(s) | 537 |
| XapI | RAATTY | 8 cut(s) | 446, 671, 819, 1273, 1590, 1788, 1906, 2068 |
| XceI | RCATGY | 1 cut(s) | 2002 |
| XmaJI | CCTAGG | 1 cut(s) | 1211 |
| XmiI | GTMKAC | 1 cut(s) | 799 |
| XspI | CTAG | 4 cut(s) | 108, 752, 1212, 2055 |