RchiOBHm_Chr7g0198811

D-arabinono-1,4-lactone oxidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000215
Physical Location & Seq
Reverse (-)
16794670 .. 16797542
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17789

Sequence Viewer

Length: 2013 bp
ATGTTTCCTAGGAAACAACTCTCAGGTTCTCAAAAAAGAAAAAGAAAGAAAAGAGAAGAAGAAATTATTCAATCTCAACGGGGATCTTTAGATAGATATTTTGTTAAAGAAACAAATGAATCTGTTGAAAATCATGTTGAGAATTTAGTAAATGAACCTGAACTACATATTCATTCTGATGAGTTTGTAGAAAATCAGAATGAGGTGAACGAAGAACTTGGTGATATAGGTGAAAATGAAAGTGATGAAATAGAAAATAATGAAAATCCTTGTCATGATGAAATTATTGCTAATGAACTTGAATGCTTGAAATCATCTGCTGATTTGAATATTTTTGATCCTAGAGTTTGGGATAGTCTAGATTCAAAAATGAGAGACTTGCTTGTAGAAAAAGGACCTATTACGGAATATGATATCAACTTTCCTAAGGATGAATTAGGTCGGCACTTTTCTGCTGAGTTTTATGTTCGAAAATTACCAAATGGTGAAACTTTTGCTAGAAAATGGTTGATTTACTTGAAAGAGCTAGATAAAGTTTTTTGTTTCTGCTGCAAATTATTTAAAACAGCTCGCTCGAGAAGTCAATTAGCAAGTGAAGGGATAAGAGATTGGAAACATCTTAGTACGACATTAAATTTACATGAAAACAGTTCTGAACACCTCATTAACTTGAGAACTTGGGCTGAGTTGCGAGTGAGATTGAATAAGAATCAAACAATTGATAAAGAGTTACAAGAGTTAATTAAGAAAGATACTGAACATTGGAAAGAGGTTATGGTCAGAATAGTTGCTGTTGTGAAATGTCTTGCAAAGAATAATTTGGCCTTTCGTGGAACAAATGAAAAAATTTATGAAGATTCTAATGGAAACTTTTTAGGCTTACTTGAAATGATTGTTGAGTTTGATCCTATAATGAAACAGCATTTTCGTCTTATTCAAGATAAAGAAATTCATTATCATTATCTTAGCCATAAAATTCAAAATGAGTTGATTGGTATGTTGGCCTTAAATGTCAAGAGTGCAATCATTAAAAAAATCAAAGAGGCAAAATATTTTTCGGTAATCCTTGATTGCACTCCTGATGCAAGTCACAAAGAACAGATGACTTTAATTATAAGATGCGTAGATGTGTTGAATTCTCCTATAAAAATAGAAGAGTTCTTTTTAGAATTTCTAAATGTGGAAGATACATCTGGTTTAGGGCTTTTTAATGAATTACAAGCAGCTTTGAAATCTCTTGATTTAGATATTGATTGTGTGCGAGGACAAGGCTATGATAATGGTTCTAATATGAAAGGAAAACACCAAGGTGTTCAAAAAAGATTGCTTGATATAAATCCTAGAGCCTTTTATATGCCGTGTGGTTGTCATTCTCTTAATTTAGTATTGTGTGACATGGCCAACTCTTGTCATAAAGCAAAATCTTTTTTTGGAACATGTCAAACAATTTATACGGTGTTCTCTAATTCTACAAAGCGATGGAGTGTTTTACTTGAATATATAGATGACTTGACTTTGAAATATTTGTCAACTACACGCTGGGAAAGTCATATTGAAAGTGTCAAAGGTATAAAAACTCAAGCTGCCCAAATAAAAGAAGCCTTAAAAAAATTGGCAGAAATTAGTGATGATGGAAAAGTGTGTAGGGACGCTGAATCTTTGATATCTGGTGAACTTTCAAGCTTTGATTTTATTTTAAGTTTGGTTATTTGGCATGACATTTTGTATAAAATTAACTTGGTTAGTAAAAAGTTACAATCCAAAGATATGCTTCTTGATGTTGCCGTGAAGAACTTAGAGGGACTAGTTTCTTATTTTGAAAAGTATAGGCAAAATGGGTTTAATTTTGCCATGACTGAAGCTAAAGAAATTGCAAATGACATGGGAGTTGAGCCTATATTTCCTATAAAACGTCACGTTTGCAGAAAAAGCATTTTGATGAAATTCCAAATACTGAAAGGGAACAACAATCTGCTCAAGAGTCCTTTAGAACTGATTACTTTTTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ALO FAD_binding_4

Protein Analysis

670

Amino Acids

77.63

Weight (kDa)

6.11

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4371 PF14291 245 - 437 1.9e-19 Domain of unknown function (DUF4371)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000215)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35150 AT2G06500 AT2G16040 AT2G19960 AT2G19960 AT3G29638 AT3G29794 AT4G10200 AT4G10200 AT5G35475
fragaria_vesca FvH4_1g08455 FvH4_1g08761 FvH4_1g10851 FvH4_1g17271 FvH4_1g26762 FvH4_2g15031 FvH4_2g18762 FvH4_2g29241 FvH4_2g32571 FvH4_4g01531 FvH4_4g12942 FvH4_4g14421 FvH4_4g14431 FvH4_4g14434 FvH4_5g30891 FvH4_6g47641 FvH4_6g47642
malus_domestica MD01G1087700.v1.1 MD11G1104400.v1.1
pyrus_communis pycom05g13550 pycom11g18320
rosa_chinensis RchiOBHm_Chr1g0320361 RchiOBHm_Chr1g0321011 RchiOBHm_Chr1g0321921 RchiOBHm_Chr1g0377611 RchiOBHm_Chr1g0379061 RchiOBHm_Chr2g0106921 RchiOBHm_Chr2g0137341 RchiOBHm_Chr2g0139641 RchiOBHm_Chr2g0139651 RchiOBHm_Chr2g0144921 RchiOBHm_Chr2g0161851 RchiOBHm_Chr2g0172511 RchiOBHm_Chr3g0449931 RchiOBHm_Chr3g0452101 RchiOBHm_Chr3g0480551 RchiOBHm_Chr4g0427821 RchiOBHm_Chr4g0431861 RchiOBHm_Chr4g0435271 RchiOBHm_Chr5g0015121 RchiOBHm_Chr5g0031951 RchiOBHm_Chr5g0034331 RchiOBHm_Chr5g0059431 RchiOBHm_Chr6g0288531 RchiOBHm_Chr6g0308901 RchiOBHm_Chr7g0198801 RchiOBHm_Chr7g0198811 RchiOBHm_Chr7g0200001 RchiOBHm_Chr7g0205811 RchiOBHm_Chr7g0230921 RchiOBHm_Chr7g0233981
rosa_multiflora Rmu_sc0000475.1_g000004 Rmu_sc0000483.1_g000021 Rmu_sc0000551.1_g000007 Rmu_sc0000761.1_g000023 Rmu_sc0000898.1_g000072 Rmu_sc0001921.1_g000031 Rmu_sc0002804.1_g000009 Rmu_sc0002880.1_g000005 Rmu_sc0003207.1_g000046 Rmu_sc0003914.1_g000010 Rmu_sc0004165.1_g000089 Rmu_sc0004209.1_g000001 Rmu_sc0004402.1_g000017 Rmu_sc0004443.1_g000001 Rmu_sc0005506.1_g000007 Rmu_sc0005506.1_g000009 Rmu_sc0006443.1_g000001 Rmu_sc0008191.1_g000019 Rmu_sc0008328.1_g000004 Rmu_sc0009489.1_g000004 Rmu_sc0010368.1_g000012 Rmu_sc0010368.1_g000014 Rmu_sc0011232.1_g000005 Rmu_sc0011630.1_g000006 Rmu_sc0014780.1_g000001 Rmu_sc0028257.1_g000002 Rmu_sc0029902.1_g000001 Rmu_ssc0000386.1_g000041 Rmu_ssc0000434.1_g000020
rosa_roxburghii Rroxscaffold_2G00084750 Rroxscaffold_2G00090380 Rroxscaffold_2G00108310 Rroxscaffold_3G00235050 Rroxscaffold_3G00252220 Rroxscaffold_4G00323580 Rroxscaffold_5G00337920 Rroxscaffold_5G00370050 Rroxscaffold_7G00169180 Rroxscaffold_7G00200470
rosa_rugosa Rorug01G0156600.1 Rorug02G0305900 Rorug02G0362300 Rorug03G0111800 Rorug03G0273500 Rorug03G0340700 Rorug04G0196000 Rorug05G0251300 Rorug05G0465700 Rorug06G0031300 Rorug06G0170700 Rorug07G0226000 Rorug07G0263200
rosa_samantha Rh1CG203400 Rh1CG203500 Rh3AG212700 Rh3BG246300 Rh4BG318100 Rh6BG118800 Rh6DG105400 Rh7DG231500
rosa_wichuraiana Rw1G002740 Rw1G016740 Rw2G009190 Rw2G021330 Rw2G030170 Rw2G034650 Rw2G034990 Rw2G046190 Rw3G019490 Rw3G019500 Rw3G019510 Rw3G019990 Rw3G020480 Rw3G026090 Rw4G005490 Rw4G007090 Rw4G022920 Rw4G030140 Rw5G011030 Rw5G019330 Rw5G027260 Rw5G029990 Rw5G034080 Rw6G025080 Rw6G031390 Rw6G038690 Rw7G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1115
AclWI GGATC 3 cut(s) 91, 332, 899
AcoI YGGCCR 1 cut(s) 1398
AcsI RAATTY 8 cut(s) 142, 634, 846, 948, 975, 1135, 1169, 1943
AcuI CTGAAG 1 cut(s) 1878
AfaI GTAC 1 cut(s) 625
AflIII ACRYGT 1 cut(s) 1436
AhlI ACTAGT 1 cut(s) 1804
AleI CACNNNNGTG 1 cut(s) 1308
AluBI AGCT 6 cut(s) 526, 569, 1226, 1583, 1683, 1862
AluI AGCT 6 cut(s) 526, 569, 1226, 1583, 1683, 1862
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 3 cut(s) 91, 332, 899
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 3 cut(s) 822, 1002, 1398
ApeKI GCWGC 3 cut(s) 549, 1223, 1583
ApoI RAATTY 8 cut(s) 142, 634, 846, 948, 975, 1135, 1169, 1943
Asp700I GAANNNNTTC 1 cut(s) 66
AspA2I CCTAGG 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 5 cut(s) 217, 233, 242, 497, 1682
AsuII TTCGAA 1 cut(s) 469
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 1 cut(s) 395
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 426
BalI TGGCCA 1 cut(s) 1400
BbvI GCAGC 3 cut(s) 536, 1235, 1570
BccI CCATC 2 cut(s) 1473, 1625
BceAI ACGGC 2 cut(s) 1342, 1769
BcoDI GTCTC 1 cut(s) 369
BcuI ACTAGT 1 cut(s) 1804
BfaI CTAG 7 cut(s) 9, 342, 359, 498, 527, 1341, 1805
BisI GCNGC 3 cut(s) 550, 1224, 1584
BlnI CCTAGG 1 cut(s) 8
BlsI GCNGC 3 cut(s) 551, 1225, 1585
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 395
BmsI GCATC 2 cut(s) 1072, 1109
Bpu14I TTCGAA 1 cut(s) 469
BpuEI CTTGAG 3 cut(s) 691, 1563, 1961
BsaBI GATNNNNATC 1 cut(s) 1335
BsaJI CCNNGG 2 cut(s) 8, 1306
Bse21I CCTNAGG 1 cut(s) 426
Bse8I GATNNNNATC 1 cut(s) 1335
BseDI CCNNGG 2 cut(s) 8, 1306
BseGI GGATG 1 cut(s) 436
BseJI GATNNNNATC 1 cut(s) 1335
BseMII CTCAG 3 cut(s) 36, 447, 675
BseXI GCAGC 3 cut(s) 536, 1235, 1570
BseYI CCCAGC 1 cut(s) 1539
BshFI GGCC 3 cut(s) 824, 1004, 1400
BsiHKCI CYCGRG 1 cut(s) 574
BslFI GGGAC 2 cut(s) 1661, 1815
BsmAI GTCTC 1 cut(s) 369
BsmFI GGGAC 2 cut(s) 1661, 1815
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 3 cut(s) 824, 1004, 1400
BsoBI CYCGRG 1 cut(s) 574
Bsp119I TTCGAA 1 cut(s) 469
Bsp143I GATC 3 cut(s) 83, 337, 904
BspANI GGCC 3 cut(s) 824, 1004, 1400
BspCNI CTCAG 3 cut(s) 35, 448, 676
BspHI TCATGA 1 cut(s) 274
BspPI GGATC 3 cut(s) 91, 332, 899
BspT104I TTCGAA 1 cut(s) 469
BssECI CCNNGG 2 cut(s) 8, 1306
BssMI GATC 3 cut(s) 83, 337, 904
BssT1I CCWWGG 2 cut(s) 8, 1306
Bst4CI ACNGT 2 cut(s) 650, 1456
Bst6I CTCTTC 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 7 cut(s) 22, 426, 456, 620, 684, 965, 1795
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 3 cut(s) 86, 340, 907
BstMAI GTCTC 1 cut(s) 369
BstMBI GATC 3 cut(s) 83, 337, 904
BstMWI GCNNNNNNNGC 1 cut(s) 1929
BstNSI RCATGY 1 cut(s) 1440
BstV1I GCAGC 3 cut(s) 536, 1235, 1570
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 3 cut(s) 824, 1004, 1400
BtgZI GCGATG 1 cut(s) 1492
BtsCI GGATG 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 571
CciI TCATGA 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 1 cut(s) 1658
Csp6I GTAC 1 cut(s) 624
CviAII CATG 8 cut(s) 134, 275, 641, 1396, 1437, 1715, 1852, 1882
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 7 cut(s) 22, 426, 456, 620, 684, 965, 1795
DpnI GATC 3 cut(s) 85, 339, 906
DpnII GATC 3 cut(s) 83, 337, 904
DraI TTTAAA 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 1398
Eam1104I CTCTTC 1 cut(s) 1149
EarI CTCTTC 1 cut(s) 1149
Eco130I CCWWGG 2 cut(s) 8, 1306
Eco32I GATATC 2 cut(s) 415, 1665
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 1878
Eco81I CCTNAGG 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 1135
EcoRV GATATC 2 cut(s) 415, 1665
EcoT14I CCWWGG 2 cut(s) 8, 1306
ErhI CCWWGG 2 cut(s) 8, 1306
FaeI CATG 8 cut(s) 137, 278, 644, 1399, 1440, 1718, 1855, 1885
FalI AAGNNNNNCTT 2 cut(s) 1755, 1787
FaqI GGGAC 2 cut(s) 1661, 1815
FatI CATG 8 cut(s) 133, 274, 640, 1395, 1436, 1714, 1851, 1881
Fnu4HI GCNGC 3 cut(s) 550, 1224, 1584
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 550, 1224, 1584
FspBI CTAG 7 cut(s) 9, 342, 359, 498, 527, 1341, 1805
GluI GCNGC 3 cut(s) 550, 1224, 1584
GsaI CCCAGC 1 cut(s) 1543
HaeIII GGCC 3 cut(s) 824, 1004, 1400
HgaI GACGC 1 cut(s) 1658
Hin1II CATG 8 cut(s) 137, 278, 644, 1399, 1440, 1718, 1855, 1885
HincII GTYRAC 1 cut(s) 1530
HindII GTYRAC 1 cut(s) 1530
HindIII AAGCTT 1 cut(s) 1681
HinfI GANTC 6 cut(s) 119, 362, 709, 857, 1655, 1981
HphI GGTGA 5 cut(s) 217, 233, 242, 497, 1682
Hpy166II GTNNAC 3 cut(s) 208, 1530, 1673
Hpy188I TCNGA 4 cut(s) 178, 198, 655, 782
Hpy188III TCNNGA 9 cut(s) 275, 359, 576, 938, 1015, 1079, 1238, 1775, 1978
Hpy8I GTNNAC 3 cut(s) 208, 1530, 1673
HpyAV CCTTC 1 cut(s) 590
HpyCH4III ACNGT 2 cut(s) 650, 1456
HpyCH4IV ACGT 2 cut(s) 1912, 1917
HpyCH4V TGCA 7 cut(s) 552, 809, 1022, 1074, 1085, 1874, 1923
HpyF10VI GCNNNNNNNGC 1 cut(s) 1929
HpyF3I CTNAG 7 cut(s) 22, 426, 456, 620, 684, 965, 1795
HpySE526I ACGT 2 cut(s) 1912, 1917
Hsp92II CATG 8 cut(s) 137, 278, 644, 1399, 1440, 1718, 1855, 1885
Kzo9I GATC 3 cut(s) 83, 337, 904
LpnPI CCDG 6 cut(s) 9, 171, 1092, 1179, 1525, 1653
Lsp1109I GCAGC 3 cut(s) 536, 1235, 1570
LweI GCATC 2 cut(s) 1072, 1109
MaeI CTAG 7 cut(s) 9, 342, 359, 498, 527, 1341, 1805
MaeII ACGT 2 cut(s) 1912, 1917
MaeIII GTNAC 5 cut(s) 729, 1088, 1391, 1752, 1913
MalI GATC 3 cut(s) 85, 339, 906
MboI GATC 3 cut(s) 83, 337, 904
MboII GAAGA 7 cut(s) 68, 71, 224, 866, 1166, 1196, 1801
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 1400
MluNI TGGCCA 1 cut(s) 1400
MlyI GAGTC 1 cut(s) 1990
MnlI CCTC 6 cut(s) 196, 671, 763, 1036, 1256, 1792
Mox20I TGGCCA 1 cut(s) 1400
MroXI GAANNNNTTC 1 cut(s) 66
MscI TGGCCA 1 cut(s) 1400
MslI CAYNNNNRTG 3 cut(s) 177, 1308, 1937
Msp20I TGGCCA 1 cut(s) 1400
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 1929
NdeII GATC 3 cut(s) 83, 337, 904
NlaIII CATG 8 cut(s) 137, 278, 644, 1399, 1440, 1718, 1855, 1885
NmuCI GTSAC 3 cut(s) 1088, 1391, 1913
NspI RCATGY 1 cut(s) 1440
NspV TTCGAA 1 cut(s) 469
OliI CACNNNNGTG 1 cut(s) 1308
PacI TTAATTAA 1 cut(s) 744
PaeR7I CTCGAG 1 cut(s) 574
PagI TCATGA 1 cut(s) 274
PciI ACATGT 1 cut(s) 1436
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 5 cut(s) 119, 362, 709, 857, 1655
PkrI GCNGC 3 cut(s) 551, 1225, 1585
PleI GAGTC 1 cut(s) 1989
PpsI GAGTC 1 cut(s) 1989
PpuMI RGGWCCY 1 cut(s) 395
PscI ACATGT 1 cut(s) 1436
PsiI TTATAA 1 cut(s) 1115
Psp5II RGGWCCY 1 cut(s) 395
PspFI CCCAGC 1 cut(s) 1539
PspPI GGNCC 1 cut(s) 395
PspPPI RGGWCCY 1 cut(s) 395
PsrI GAACNNNNNNTAC 2 cut(s) 147, 179
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
RseI CAYNNNNRTG 3 cut(s) 177, 1308, 1937
SatI GCNGC 3 cut(s) 550, 1224, 1584
Sau3AI GATC 3 cut(s) 83, 337, 904
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 1990
SfaNI GCATC 2 cut(s) 1072, 1109
Sfr274I CTCGAG 1 cut(s) 574
SfuI TTCGAA 1 cut(s) 469
SinI GGWCC 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 3 cut(s) 177, 1308, 1937
SmlI CTYRAG 4 cut(s) 574, 670, 1578, 1976
SmoI CTYRAG 4 cut(s) 574, 670, 1578, 1976
SpeI ACTAGT 1 cut(s) 1804
SspI AATATT 3 cut(s) 331, 1052, 1523
SspMI CTAG 7 cut(s) 9, 342, 359, 498, 527, 1341, 1805
StyI CCWWGG 2 cut(s) 8, 1306
TaaI ACNGT 2 cut(s) 650, 1456
TaiI ACGT 2 cut(s) 1915, 1920
TaqI TCGA 2 cut(s) 469, 575
TfiI GAWTC 5 cut(s) 119, 362, 709, 857, 1655
TseFI GTSAC 3 cut(s) 1088, 1391, 1913
TseI GCWGC 3 cut(s) 549, 1223, 1583
Tsp45I GTSAC 3 cut(s) 1088, 1391, 1913
TspGWI ACGGA 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 8 cut(s) 142, 634, 846, 948, 975, 1135, 1169, 1943
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 1 cut(s) 1440
XhoI CTCGAG 1 cut(s) 574
XmaJI CCTAGG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 7 cut(s) 9, 342, 359, 498, 527, 1341, 1805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.