pycom12g02500

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000155
Physical Location & Seq
Forward (+)
2169259 .. 2171467
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02500.2

Sequence Viewer

Length: 1440 bp
ATGGAACACAAGGATAAGTTATTGGCTATTGTGAATTCTGAAGCTCAAGGACTCTGTCCCGAGAGTGTAGTGGACAGGTTTAGTAATCTTCCAGATGAAGTCGCTCATCAGATTCTTTCACTTGTCTCTTTAAAAGAACTCATTCGAGTAGGCAGCCTTTCTAAAAGATGCAGAGGGCTTTATCTCTCAACCCCATCATTGAAATTTTGGTCATCGTACTATGGAGATAAGCAGGGCCAATTAAACTTGCTGAATTCTTTCGATAGGCTTTTGATTCTTCGCGGAGGTAATAAGGTACAGAAGTTTCAAGTACATTTGGATCTCTGTTCAAGCTTACCTGATGAGATTTTCCGAGTGATCACGTGGATCCACATTGCAGCAAGGTGTAATGTTGAAGTGCTTGATCTTCAGTTGAGGGTATACAAAAATGATATGGCTTCGTTTGAATTACCATCTTGCATCTTTCTTTGTGGATCTTTGAGATCTCTGATTGTGACTCTGTATACAGTTCTTAAAGTTCCCTCCTTTGCCGGTTCCACTAATCTCCGACACTTAAACTTGAGTTATGTTCAAATAGATGACGGCTTTTCCAAATGGATCTCAAATTCCTGCAAATGTATCAAGGAGTTGCAGCTTTATCATGTTGGAGCAGTAAATATCACCATTGAAAGCTCATCTTTGGAATCAGTTAGGTTTGTTTCGGTTCAGAATGACACCTGCCATCTTAACATCACAGGTGAGAAACTTGAAGATATACATGTTAATTGGGAATATTGTCAACGTGGCAGCAGATCATTAACTATTTCTGCTCCAAATCTCAAATATTTCAAATGGATTGGGAGTTTATTGAACCGCCAGAATCTCAGGGAATTAATGTGTTTAGAAAAGGCAGAGATTTTTTTGAAGCCTAGGAGAGGTATCTGTGAAAACTTTGACAACGCATCCGAGTTTCTATGCAGTATATGCAGGGTTAAAGTTCTTCTTCTAAGCCACGAGACCATTAAGACTCTGTTCAAGGAAGTTTCCATACCCCCGCCGTTTGATAATATTTCTTACTTGGGTTTGCGCATTACGAGCTTGAGTGATGACCTAGTCCCAGCAATGGTATCTCTTTTGAGAGGAATTTCTAATTTGAATACATTGCAAATAGAATCTGACCTTTCCTTACTCTTCCATAAACCTAAAGCACGCGGATGTAATAGGAAATACTGGAAATCCCAAAACCTGAATTTTACAGATCAGCTTAAGGAGGTAACAATAGAGCTTTCCAACGGGTTCAATGCCATGGAGTTAGCAAGGTATATTCTCGAGCAAGCTCAGCATTTGGAGAAAATGTTCATATTCTATTTACCCCACCAGTCATATGTCATAAAAAGAGTAAATCAAAGCAAGAAAGTCTCCGCTGCCCAAATTGTCTTTCGGAAGAAACAGCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

480

Amino Acids

54.86

Weight (kDa)

9.02

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000155)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20530 FvH4_5g20530 FvH4_5g20611 FvH4_5g20621 FvH4_5g26390 FvH4_5g26400 FvH4_5g26410 FvH4_5g29160 FvH4_5g29160 FvH4_5g29160 FvH4_5g29160 FvH4_6g18920 FvH4_6g18920 FvH4_6g18920 FvH4_6g33960
malus_domestica MD04G1058800.v1.1 MD12G1025400.v1.1 MD14G1024100.v1.1 MD14G1026000.v1.1 MD14G1026300.v1.1 MD14G1026400.v1.1 MD14G1026600.v1.1
prunus_persica Prupe.7G107300_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108700_v2.0.a1
pyrus_communis pycom04g05270 pycom12g02490 pycom12g02500 pycom14g02340 pycom14g02540
rosa_chinensis RchiOBHm_Chr3g0473911 RchiOBHm_Chr3g0473951 RchiOBHm_Chr3g0473961 RchiOBHm_Chr3g0474091 RchiOBHm_Chr3g0474151 RchiOBHm_Chr3g0474171 RchiOBHm_Chr3g0475381 RchiOBHm_Chr3g0475391 RchiOBHm_Chr7g0193261 RchiOBHm_Chr7g0205891 RchiOBHm_Chr7g0205901 RchiOBHm_Chr7g0205931 RchiOBHm_Chr7g0205941 RchiOBHm_Chr7g0205961 RchiOBHm_Chr7g0217271 RchiOBHm_Chr7g0217281
rosa_laevigata RLG00000002499 RLG00000003343 RLG00000003344 RLG00000003345 RLG00000003348 RLG00000004282 RLG00000023847 RLG00000023943 RLG00000023945 RLG00000023951 RLG00000023964 RLG00000023965 RLG00000023969 RLG00000023971
rosa_multiflora Rmu_co8482831.1_g000001 Rmu_sc0000076.1_g000028 Rmu_sc0000076.1_g000030 Rmu_sc0000076.1_g000032 Rmu_sc0000076.1_g000033 Rmu_sc0002414.1_g000019 Rmu_sc0002414.1_g000037 Rmu_sc0003170.1_g000001 Rmu_sc0003170.1_g000026 Rmu_sc0004084.1_g000005 Rmu_sc0004084.1_g000006 Rmu_sc0004084.1_g000014 Rmu_sc0004507.1_g000027 Rmu_sc0005137.1_g000001 Rmu_sc0005137.1_g000022 Rmu_sc0005137.1_g000038 Rmu_sc0007659.1_g000002 Rmu_sc0010415.1_g000002 Rmu_sc0013923.1_g000002 Rmu_sc0015051.1_g000001 Rmu_sc0016637.1_g000001 Rmu_sc0016637.1_g000003 Rmu_sc0018174.1_g000001 Rmu_sc0033900.1_g000001
rosa_roxburghii Rroxscaffold_3G00242480 Rroxscaffold_3G00251850 Rroxscaffold_3G00251880 Rroxscaffold_3G00251890 Rroxscaffold_3G00262240 Rroxscaffold_6G00406350 Rroxscaffold_6G00407490 Rroxscaffold_6G00407590 Rroxscaffold_6G00407650
rosa_rugosa Rorug03G0136900 Rorug03G0136900 Rorug03G0136900 Rorug03G0137000 Rorug03G0137200 Rorug03G0137400 Rorug03G0138400 Rorug03G0138700 Rorug03G0138700 Rorug03G0139000 Rorug03G0139100 Rorug03G0148300 Rorug03G0148400 Rorug03G0148500 Rorug07G0008600 Rorug07G0093700 Rorug07G0093800 Rorug07G0093900 Rorug07G0094000 Rorug07G0167300
rosa_samantha Rh3AG187100 Rh3AG187500 Rh3AG188600 Rh3AG189500 Rh3AG198400 Rh3AG198500 Rh3AG198600 Rh3BG215700 Rh3BG215800 Rh3BG216000 Rh3BG218100 Rh3BG218700 Rh3BG228400 Rh3BG228500 Rh3BG228600 Rh3CG212400 Rh3CG212600 Rh3CG213800 Rh3CG214300 Rh3CG222900 Rh3DG211600 Rh3DG211900 Rh3DG212100 Rh3DG213900 Rh3DG214100 Rh3DG223700 Rh3DG223900 Rh7AG134200 Rh7AG225400 Rh7AG225500 Rh7AG225600 Rh7AG225700 Rh7AG306800 Rh7BG135200 Rh7BG221300 Rh7BG221400 Rh7BG221500 Rh7BG221700 Rh7BG221900 Rh7BG222300 Rh7BG225100 Rh7BG225200 Rh7BG297700 Rh7CG138200 Rh7CG239000 Rh7CG239200 Rh7CG239500 Rh7CG239800 Rh7CG240100 Rh7CG325900 Rh7DG232100 Rh7DG232300 Rh7DG306500
rosa_wichuraiana Rw0G012880 Rw0G012890 Rw3G017130 Rw3G017180 Rw3G017300 Rw3G017310 Rw3G018020 Rw7G011480 Rw7G019440 Rw7G019450 Rw7G019470 Rw7G026050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 725
Acc16I TGCGCA 1 cut(s) 1067
Acc36I ACCTGC 1 cut(s) 725
AccI GTMKAC 2 cut(s) 420, 503
AccII CGCG 2 cut(s) 282, 1191
AciI CCGC 5 cut(s) 282, 853, 1034, 1191, 1401
AclWI GGATC 5 cut(s) 327, 361, 374, 481, 605
AcsI RAATTY 6 cut(s) 34, 203, 253, 604, 1122, 1228
AcuI CTGAAG 2 cut(s) 60, 392
AcvI CACGTG 1 cut(s) 363
AfaI GTAC 3 cut(s) 218, 297, 312
AfiI CCNNNNNNNGG 2 cut(s) 914, 1102
AflII CTTAAG 1 cut(s) 1244
AflIII ACRYGT 1 cut(s) 757
AjuI GAANNNNNNNTTGG 2 cut(s) 1401, 1433
AloI GAACNNNNNNTCC 2 cut(s) 1319, 1351
AluBI AGCT 8 cut(s) 44, 333, 634, 672, 1077, 1243, 1264, 1316
AluI AGCT 8 cut(s) 44, 333, 634, 672, 1077, 1243, 1264, 1316
Alw26I GTCTC 3 cut(s) 130, 989, 1402
AlwI GGATC 5 cut(s) 327, 361, 374, 481, 605
Ama87I CYCGRG 2 cut(s) 59, 1307
AoxI GGCC 1 cut(s) 235
ApeKI GCWGC 5 cut(s) 153, 377, 631, 786, 1403
ApoI RAATTY 6 cut(s) 34, 203, 253, 604, 1122, 1228
AseI ATTAAT 1 cut(s) 872
Asp700I GAANNNNTTC 3 cut(s) 141, 257, 1334
AspA2I CCTAGG 1 cut(s) 908
AspLEI GCGC 1 cut(s) 1068
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 652, 749
AvaI CYCGRG 2 cut(s) 59, 1307
AvrII CCTAGG 1 cut(s) 908
BaeI ACNNNNGTAYC 2 cut(s) 287, 320
BamHI GGATCC 1 cut(s) 366
BarI GAAGNNNNNNTAC 2 cut(s) 1011, 1043
BauI CACGAG 1 cut(s) 992
BbrPI CACGTG 1 cut(s) 363
BbvI GCAGC 5 cut(s) 165, 389, 643, 798, 1390
BccI CCATC 3 cut(s) 202, 460, 729
BceAI ACGGC 2 cut(s) 598, 1021
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 3 cut(s) 130, 989, 1402
BfaI CTAG 2 cut(s) 909, 1091
BfrI CTTAAG 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 725
BglII AGATCT 1 cut(s) 482
BisI GCNGC 5 cut(s) 154, 378, 632, 787, 1404
BlnI CCTAGG 1 cut(s) 908
BlpI GCTNAGC 1 cut(s) 1317
BlsI GCNGC 5 cut(s) 155, 379, 633, 788, 1405
BmeT110I CYCGRG 2 cut(s) 59, 1307
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 368, 535
BmsI GCATC 3 cut(s) 158, 468, 950
Bpu1102I GCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 3 cut(s) 30, 580, 1099
BsaAI YACGTR 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 2 cut(s) 908, 1284
BsaXI ACNNNNNCTCC 2 cut(s) 1319, 1349
Bsc4I CCNNNNNNNGG 2 cut(s) 914, 1102
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 1214, 1357
Bse3DI GCAATG 3 cut(s) 372, 1107, 1139
BseDI CCNNGG 2 cut(s) 908, 1284
BseGI GGATG 2 cut(s) 941, 1199
BseLI CCNNNNNNNGG 2 cut(s) 914, 1102
BseMI GCAATG 3 cut(s) 372, 1107, 1139
BseMII CTCAG 2 cut(s) 877, 1331
BseNI ACTGG 2 cut(s) 1214, 1357
BseXI GCAGC 5 cut(s) 165, 389, 643, 798, 1390
BseYI CCCAGC 1 cut(s) 1096
Bsh1236I CGCG 2 cut(s) 282, 1191
BshFI GGCC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 59, 1307
BsiSI CCGG 1 cut(s) 531
BslFI GGGAC 2 cut(s) 42, 1079
BslI CCNNNNNNNGG 2 cut(s) 914, 1102
BsmAI GTCTC 3 cut(s) 130, 989, 1402
BsmFI GGGAC 2 cut(s) 42, 1079
BsnI GGCC 1 cut(s) 237
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 2 cut(s) 59, 1307
Bsp143I GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
Bsp1720I GCTNAGC 1 cut(s) 1317
Bsp19I CCATGG 1 cut(s) 1284
BspACI CCGC 5 cut(s) 282, 853, 1034, 1191, 1401
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 2 cut(s) 876, 1330
BspFNI CGCG 2 cut(s) 282, 1191
BspLI GGNNCC 2 cut(s) 368, 535
BspMI ACCTGC 1 cut(s) 725
BspPI GGATC 5 cut(s) 327, 361, 374, 481, 605
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 989
BsrDI GCAATG 3 cut(s) 372, 1107, 1139
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 2 cut(s) 1214, 1357
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 2 cut(s) 908, 1284
BssMI GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
BssNAI GTATAC 2 cut(s) 421, 504
BssSI CACGAG 1 cut(s) 992
BssT1I CCWWGG 2 cut(s) 908, 1284
Bst1107I GTATAC 2 cut(s) 421, 504
Bst2BI CACGAG 1 cut(s) 992
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 1175
BstAFI CTTAAG 1 cut(s) 1244
BstAPI GCANNNNNTGC 1 cut(s) 963
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 1189, 1314
BstDEI CTNAG 3 cut(s) 863, 986, 1317
BstDSI CCRYGG 1 cut(s) 1284
BstENI CCTNNNNNAGG 1 cut(s) 912
BstF5I GGATG 2 cut(s) 941, 1199
BstFNI CGCG 2 cut(s) 282, 1191
BstHHI GCGC 1 cut(s) 1068
BstKTI GATC 9 cut(s) 322, 360, 369, 406, 476, 485, 600, 794, 1240
BstMAI GTCTC 3 cut(s) 130, 989, 1402
BstMBI GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
BstMWI GCNNNNNNNGC 3 cut(s) 963, 1074, 1318
BstNSI RCATGY 1 cut(s) 761
BstUI CGCG 2 cut(s) 282, 1191
BstV1I GCAGC 5 cut(s) 165, 389, 643, 798, 1390
BstX2I RGATCY 5 cut(s) 319, 366, 473, 482, 597
BstYI RGATCY 5 cut(s) 319, 366, 473, 482, 597
BstZ17I GTATAC 2 cut(s) 421, 504
BsuRI GGCC 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 1284
BtsCI GGATG 2 cut(s) 941, 1199
BveI ACCTGC 1 cut(s) 725
Cac8I GCNNGC 2 cut(s) 1189, 1314
CfoI GCGC 1 cut(s) 1068
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 3 cut(s) 217, 296, 311
CviAII CATG 3 cut(s) 641, 758, 1285
CviQI GTAC 3 cut(s) 217, 296, 311
DdeI CTNAG 3 cut(s) 863, 986, 1317
DpnI GATC 9 cut(s) 321, 359, 368, 405, 475, 484, 599, 793, 1239
DpnII GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
DraI TTTAAA 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 1175
EarI CTCTTC 1 cut(s) 1175
Eco130I CCWWGG 2 cut(s) 908, 1284
Eco31I GGTCTC 1 cut(s) 989
Eco57I CTGAAG 2 cut(s) 60, 392
Eco72I CACGTG 1 cut(s) 363
Eco88I CYCGRG 2 cut(s) 59, 1307
EcoNI CCTNNNNNAGG 1 cut(s) 912
EcoRI GAATTC 2 cut(s) 34, 253
EcoT14I CCWWGG 2 cut(s) 908, 1284
ErhI CCWWGG 2 cut(s) 908, 1284
FaeI CATG 3 cut(s) 644, 761, 1288
FalI AAGNNNNNCTT 4 cut(s) 661, 693, 966, 998
FaqI GGGAC 2 cut(s) 42, 1079
FatI CATG 3 cut(s) 640, 757, 1284
FauI CCCGC 1 cut(s) 1041
FauNDI CATATG 1 cut(s) 1363
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 2 cut(s) 420, 503
Fnu4HI GCNGC 5 cut(s) 154, 378, 632, 787, 1404
FokI GGATG 2 cut(s) 928, 1206
Fsp4HI GCNGC 5 cut(s) 154, 378, 632, 787, 1404
FspBI CTAG 2 cut(s) 909, 1091
FspI TGCGCA 1 cut(s) 1067
GlaI GCGC 1 cut(s) 1067
GluI GCNGC 5 cut(s) 154, 378, 632, 787, 1404
GsaI CCCAGC 1 cut(s) 1100
HaeIII GGCC 1 cut(s) 237
HapII CCGG 1 cut(s) 531
HhaI GCGC 1 cut(s) 1068
Hin1II CATG 3 cut(s) 644, 761, 1288
Hin6I GCGC 1 cut(s) 1066
HinP1I GCGC 1 cut(s) 1066
HincII GTYRAC 1 cut(s) 779
HindII GTYRAC 1 cut(s) 779
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 8 cut(s) 51, 112, 274, 496, 683, 859, 1006, 1151
HpaII CCGG 1 cut(s) 531
HphI GGTGA 2 cut(s) 652, 749
Hpy166II GTNNAC 4 cut(s) 73, 421, 504, 779
Hpy188I TCNGA 9 cut(s) 40, 111, 353, 489, 548, 708, 946, 1156, 1422
Hpy188III TCNNGA 3 cut(s) 59, 92, 1307
Hpy8I GTNNAC 4 cut(s) 73, 421, 504, 779
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 2 cut(s) 362, 781
HpyCH4V TGCA 8 cut(s) 171, 377, 459, 612, 631, 957, 966, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 963, 1074, 1318
HpyF3I CTNAG 3 cut(s) 863, 986, 1317
HpySE526I ACGT 2 cut(s) 362, 781
Hsp92II CATG 3 cut(s) 644, 761, 1288
HspAI GCGC 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
LmnI GCTCC 2 cut(s) 647, 814
Lsp1109I GCAGC 5 cut(s) 165, 389, 643, 798, 1390
LweI GCATC 3 cut(s) 158, 468, 950
MaeI CTAG 2 cut(s) 909, 1091
MaeII ACGT 2 cut(s) 362, 781
MaeIII GTNAC 2 cut(s) 493, 1252
MalI GATC 9 cut(s) 321, 359, 368, 405, 475, 484, 599, 793, 1239
MboI GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
MboII GAAGA 8 cut(s) 80, 269, 398, 761, 971, 974, 1162, 1435
MflI RGATCY 5 cut(s) 319, 366, 473, 482, 597
MlyI GAGTC 3 cut(s) 45, 490, 1000
MmeI TCCRAC 3 cut(s) 571, 625, 1293
MnlI CCTC 7 cut(s) 167, 278, 408, 532, 908, 1112, 1243
MroXI GAANNNNTTC 3 cut(s) 141, 257, 1334
MslI CAYNNNNRTG 1 cut(s) 1192
MspA1I CMGCKG 1 cut(s) 1403
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 1 cut(s) 531
MvnI CGCG 2 cut(s) 282, 1191
MwoI GCNNNNNNNGC 3 cut(s) 963, 1074, 1318
NcoI CCATGG 1 cut(s) 1284
NdeI CATATG 1 cut(s) 1363
NdeII GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
NlaIII CATG 3 cut(s) 644, 761, 1288
NlaIV GGNNCC 2 cut(s) 368, 535
NmuCI GTSAC 1 cut(s) 493
NsbI TGCGCA 1 cut(s) 1067
NspI RCATGY 1 cut(s) 761
PaeR7I CTCGAG 1 cut(s) 1307
PaqCI CACCTGC 1 cut(s) 725
PciI ACATGT 1 cut(s) 757
PdmI GAANNNNTTC 3 cut(s) 141, 257, 1334
PfeI GAWTC 5 cut(s) 112, 274, 683, 859, 1151
PflFI GACNNNGTC 2 cut(s) 54, 1091
PkrI GCNGC 5 cut(s) 155, 379, 633, 788, 1405
PleI GAGTC 3 cut(s) 45, 490, 1000
PmaCI CACGTG 1 cut(s) 363
PmlI CACGTG 1 cut(s) 363
PpsI GAGTC 3 cut(s) 45, 490, 1000
Ppu21I YACGTR 1 cut(s) 363
PscI ACATGT 1 cut(s) 757
PshBI ATTAAT 1 cut(s) 872
PspCI CACGTG 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 1096
PspN4I GGNNCC 2 cut(s) 368, 535
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 5 cut(s) 319, 366, 473, 482, 597
PsyI GACNNNGTC 2 cut(s) 54, 1091
RsaI GTAC 3 cut(s) 218, 297, 312
RsaNI GTAC 3 cut(s) 217, 296, 311
RseI CAYNNNNRTG 1 cut(s) 1192
SatI GCNGC 5 cut(s) 154, 378, 632, 787, 1404
Sau3AI GATC 9 cut(s) 319, 357, 366, 403, 473, 482, 597, 791, 1237
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 3 cut(s) 45, 490, 1000
SfaNI GCATC 3 cut(s) 158, 468, 950
Sfr274I CTCGAG 1 cut(s) 1307
SlaI CTCGAG 1 cut(s) 1307
SmiMI CAYNNNNRTG 1 cut(s) 1192
SmlI CTYRAG 5 cut(s) 45, 559, 1078, 1244, 1307
SmoI CTYRAG 5 cut(s) 45, 559, 1078, 1244, 1307
SsiI CCGC 5 cut(s) 282, 853, 1034, 1191, 1401
SspI AATATT 3 cut(s) 773, 824, 1048
SspMI CTAG 2 cut(s) 909, 1091
StyI CCWWGG 2 cut(s) 908, 1284
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 2 cut(s) 365, 784
TaqI TCGA 3 cut(s) 145, 261, 1308
TatI WGTACW 1 cut(s) 310
TfiI GAWTC 5 cut(s) 112, 274, 683, 859, 1151
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 5 cut(s) 153, 377, 631, 786, 1403
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 111, 1327
Tth111I GACNNNGTC 2 cut(s) 54, 1091
Vha464I CTTAAG 1 cut(s) 1244
VspI ATTAAT 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 912
XapI RAATTY 6 cut(s) 34, 203, 253, 604, 1122, 1228
XceI RCATGY 1 cut(s) 761
XhoI CTCGAG 1 cut(s) 1307
XmaJI CCTAGG 1 cut(s) 908
XmiI GTMKAC 2 cut(s) 420, 503
XmnI GAANNNNTTC 3 cut(s) 141, 257, 1334
XspI CTAG 2 cut(s) 909, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.