Rroxscaffold_3G00262240

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000155
Physical Location & Seq
Forward (+)
56285422 .. 56289852
4431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262240.1

Sequence Viewer

Length: 1641 bp
ATGGAACGCCAGCATAAGGTATTGGCAACTACAAGTAATCAAGGCCAAGGTGCTAGCAGTGAGAGTTTAGTTGATAGATTTAGTTGTCTTCCGGAAGAAGTTGTCTGTCACATTCTTTCCTATCTCAATGCATTGGATCTCATTCGAGCGAGCAGTGTGTCAAAACGATGCAGAATGTTATATGTTTCAGTCCCGGGATTAAATTTCGATGTAAATGCGCTGCCCGGAGGGAAATGCTGGTGGAAGCGATCAAAATTGTTGAATCATTTGGATAGGTTATTGATTCAACGCGAGGGTAGCAAGACAGAAAGGTTTCGTATCAGTTGGGTTTTTGAAGGAGTAACTGCATGCAACTGTGAGGAGCATTGTCGAGTGATCACGTGGATCTATACTGCTGTGAAGTGTAATGTTGAAGAGTTAGATCTTCAGCTAGCTCTGAACTATAGAGGGAAAACAACAAGTTTAGACTTGCAATGTTGCACCTTCCGTTGTCCATCTTTGAGGTCCCTAACGCTGGACTTGAAGTTCTGTGTTCTTAAAGCGCCCTCAATCACCATTTGCACTAATCTCCAATCCTTACTATTAAGAAGTGTTATCATTGAGGAGGATTTTTTTAAATGGATATCGAGTTCATGTCAATGCATCAAGGAGTTAACTATTGACTGTGTTCTTAGGCCGAAAAGTATCAGCATTGAGAGCCCTTCTTTGGAATCCATTCATTTTGTGTTTGTGGGTAGACGTCAACAATACGAAGACATTGATGCTCTTAGCATTTCTGGTGAAAATCTTCATCGTATACATATTGAGCATATTGACCTGAGATCTTCAGCATGCCACCATTCATTATATCTTGTGGCTCCAAATCTTAAATATTTGAATTTAACTGGGAATGTTGCTCGTGGTCTATCTGTGGAGAAATTCGTACATTCAGAAGAAGCTATGATATTTCTCAACCCGGGGGAAGATGGTTTTGACAAAGTATTTGAAGTGGTTAGCTGTATACTTGGGGTTAAAGTTCTTCATCTAAACACAGTGACCATAAAGGCTCTATTCAAATTTGGGGAGGGATCCATGCCAGCACTATTTTACAATATCCGTCACCTCTATATCCATACCGAGGGCTTGGAGAATGACCAAGTCTCAGCACTGGTCTCTCTTCTAAGTGAAACGCCAAATGTGAGTACTTTAAACATGAAGTGTGAGCCGAATACTAGGCAATTAAAATCTGTGGAACCGACTCGTGTATCTGTGGGTAAAAAGTGGAAAGCCCAAAGCGTGGCTTGTATTGACCAACTTAAGGGGGCGACATTAGAGATTTCCTATGGAAATAATGAATTGGAGTTAGCGAGGTACTTGTTGGAGGATGCTAAGAAGTTGAGAAAGATGGTCTTACAATTGAGTGCCTGGTCATACTCTCGGAAGAAATTGAAGCTAGCGAGGGAGTTGCCGTATCCATCAAATCTTATTAGGGCAGTAAACGAAGACAAGATTAATTCTACTGCCACAATTGTCTTTGAGGCTGCTTTAGATAACAAAGAAGGAAAAACCAAGGCCTACAATGTGGGGTGTCAAGGCACTCAGGAGAGTTCAGACCTTGAGTTTTATGCTGAAGGTTCACCAGAGAACGAGAATAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

546

Amino Acids

61.59

Weight (kDa)

7.75

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 27 - 59 4.6e-09 F-box domain
F-box-like PF12937 27 - 58 1.3e-07 F-box-like
LRR_At5g56370 PF24758 127 - 308 4.9e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000155)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20530 FvH4_5g20530 FvH4_5g20611 FvH4_5g20621 FvH4_5g26390 FvH4_5g26400 FvH4_5g26410 FvH4_5g29160 FvH4_5g29160 FvH4_5g29160 FvH4_5g29160 FvH4_6g18920 FvH4_6g18920 FvH4_6g18920 FvH4_6g33960
malus_domestica MD04G1058800.v1.1 MD12G1025400.v1.1 MD14G1024100.v1.1 MD14G1026000.v1.1 MD14G1026300.v1.1 MD14G1026400.v1.1 MD14G1026600.v1.1
prunus_persica Prupe.7G107300_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108600_v2.0.a1 Prupe.7G108700_v2.0.a1
pyrus_communis pycom04g05270 pycom12g02490 pycom12g02500 pycom14g02340 pycom14g02540
rosa_chinensis RchiOBHm_Chr3g0473911 RchiOBHm_Chr3g0473951 RchiOBHm_Chr3g0473961 RchiOBHm_Chr3g0474091 RchiOBHm_Chr3g0474151 RchiOBHm_Chr3g0474171 RchiOBHm_Chr3g0475381 RchiOBHm_Chr3g0475391 RchiOBHm_Chr7g0193261 RchiOBHm_Chr7g0205891 RchiOBHm_Chr7g0205901 RchiOBHm_Chr7g0205931 RchiOBHm_Chr7g0205941 RchiOBHm_Chr7g0205961 RchiOBHm_Chr7g0217271 RchiOBHm_Chr7g0217281
rosa_laevigata RLG00000002499 RLG00000003343 RLG00000003344 RLG00000003345 RLG00000003348 RLG00000004282 RLG00000023847 RLG00000023943 RLG00000023945 RLG00000023951 RLG00000023964 RLG00000023965 RLG00000023969 RLG00000023971
rosa_multiflora Rmu_co8482831.1_g000001 Rmu_sc0000076.1_g000028 Rmu_sc0000076.1_g000030 Rmu_sc0000076.1_g000032 Rmu_sc0000076.1_g000033 Rmu_sc0002414.1_g000019 Rmu_sc0002414.1_g000037 Rmu_sc0003170.1_g000001 Rmu_sc0003170.1_g000026 Rmu_sc0004084.1_g000005 Rmu_sc0004084.1_g000006 Rmu_sc0004084.1_g000014 Rmu_sc0004507.1_g000027 Rmu_sc0005137.1_g000001 Rmu_sc0005137.1_g000022 Rmu_sc0005137.1_g000038 Rmu_sc0007659.1_g000002 Rmu_sc0010415.1_g000002 Rmu_sc0013923.1_g000002 Rmu_sc0015051.1_g000001 Rmu_sc0016637.1_g000001 Rmu_sc0016637.1_g000003 Rmu_sc0018174.1_g000001 Rmu_sc0033900.1_g000001
rosa_roxburghii Rroxscaffold_3G00242480 Rroxscaffold_3G00251850 Rroxscaffold_3G00251880 Rroxscaffold_3G00251890 Rroxscaffold_3G00262240 Rroxscaffold_6G00406350 Rroxscaffold_6G00407490 Rroxscaffold_6G00407590 Rroxscaffold_6G00407650
rosa_rugosa Rorug03G0136900 Rorug03G0136900 Rorug03G0136900 Rorug03G0137000 Rorug03G0137200 Rorug03G0137400 Rorug03G0138400 Rorug03G0138700 Rorug03G0138700 Rorug03G0139000 Rorug03G0139100 Rorug03G0148300 Rorug03G0148400 Rorug03G0148500 Rorug07G0008600 Rorug07G0093700 Rorug07G0093800 Rorug07G0093900 Rorug07G0094000 Rorug07G0167300
rosa_samantha Rh3AG187100 Rh3AG187500 Rh3AG188600 Rh3AG189500 Rh3AG198400 Rh3AG198500 Rh3AG198600 Rh3BG215700 Rh3BG215800 Rh3BG216000 Rh3BG218100 Rh3BG218700 Rh3BG228400 Rh3BG228500 Rh3BG228600 Rh3CG212400 Rh3CG212600 Rh3CG213800 Rh3CG214300 Rh3CG222900 Rh3DG211600 Rh3DG211900 Rh3DG212100 Rh3DG213900 Rh3DG214100 Rh3DG223700 Rh3DG223900 Rh7AG134200 Rh7AG225400 Rh7AG225500 Rh7AG225600 Rh7AG225700 Rh7AG306800 Rh7BG135200 Rh7BG221300 Rh7BG221400 Rh7BG221500 Rh7BG221700 Rh7BG221900 Rh7BG222300 Rh7BG225100 Rh7BG225200 Rh7BG297700 Rh7CG138200 Rh7CG239000 Rh7CG239200 Rh7CG239500 Rh7CG239800 Rh7CG240100 Rh7CG325900 Rh7DG232100 Rh7DG232300 Rh7DG306500
rosa_wichuraiana Rw0G012880 Rw0G012890 Rw3G017130 Rw3G017180 Rw3G017300 Rw3G017310 Rw3G018020 Rw7G011480 Rw7G019440 Rw7G019450 Rw7G019470 Rw7G026050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 742
AccB7I CCANNNNNTGG 1 cut(s) 1276
AccI GTMKAC 3 cut(s) 736, 796, 1000
AccII CGCG 1 cut(s) 291
AccIII TCCGGA 1 cut(s) 91
AclWI GGATC 4 cut(s) 144, 392, 1062, 1075
AcsI RAATTY 4 cut(s) 202, 877, 917, 1055
AcuI CTGAAG 3 cut(s) 410, 810, 1629
AcvI CACGTG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 739
AfaI GTAC 3 cut(s) 924, 1183, 1352
AfiI CCNNNNNNNGG 6 cut(s) 16, 514, 706, 1117, 1276, 1297
AflII CTTAAG 1 cut(s) 1295
AgsI TTSAA 9 cut(s) 262, 287, 335, 413, 523, 877, 986, 1054, 1429
AjnI CCWGG 1 cut(s) 1403
AloI GAACNNNNNNTCC 4 cut(s) 509, 541, 613, 645
AluBI AGCT 5 cut(s) 430, 434, 938, 996, 1432
AluI AGCT 5 cut(s) 430, 434, 938, 996, 1432
Alw26I GTCTC 2 cut(s) 1144, 1156
AlwI GGATC 4 cut(s) 144, 392, 1062, 1075
Ama87I CYCGRG 2 cut(s) 193, 955
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 3 cut(s) 43, 674, 1551
ApeKI GCWGC 2 cut(s) 220, 1520
ApoI RAATTY 4 cut(s) 202, 877, 917, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AseI ATTAAT 1 cut(s) 1491
Asp700I GAANNNNTTC 3 cut(s) 312, 714, 786
AspLEI GCGC 2 cut(s) 220, 544
AspS9I GGNCC 1 cut(s) 504
AsuC2I CCSGG 5 cut(s) 194, 195, 225, 956, 957
AsuHPI GGTGA 4 cut(s) 544, 791, 1091, 1608
AsuNHI GCTAGC 3 cut(s) 53, 430, 1432
AvaI CYCGRG 2 cut(s) 193, 955
AvaII GGWCC 1 cut(s) 504
BaeI ACNNNNGTAYC 2 cut(s) 1227, 1260
BamHI GGATCC 1 cut(s) 1067
BanII GRGCYC 1 cut(s) 701
BauI CACGAG 2 cut(s) 897, 1239
BbrPI CACGTG 1 cut(s) 381
BbsI GAAGAC 3 cut(s) 80, 759, 1488
BbvI GCAGC 2 cut(s) 207, 1507
BccI CCATC 4 cut(s) 502, 959, 1378, 1462
BceAI ACGGC 1 cut(s) 1432
BcgI CGANNNNNNTGC 4 cut(s) 197, 231, 1426, 1460
BciT130I CCWGG 1 cut(s) 1405
BciVI GTATCC 1 cut(s) 1461
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 5 cut(s) 194, 195, 225, 956, 957
BcoDI GTCTC 2 cut(s) 1144, 1156
BfaI CTAG 4 cut(s) 54, 431, 1212, 1433
BfmI CTRYAG 1 cut(s) 442
BfoI RGCGCY 1 cut(s) 545
BfrI CTTAAG 1 cut(s) 1295
BfuI GTATCC 1 cut(s) 1461
BglII AGATCT 2 cut(s) 421, 821
BisI GCNGC 2 cut(s) 221, 1521
BlsI GCNGC 2 cut(s) 222, 1522
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 6 cut(s) 194, 195, 225, 956, 957, 1405
Bme18I GGWCC 1 cut(s) 504
BmeT110I CYCGRG 2 cut(s) 193, 955
BmgT120I GGNCC 1 cut(s) 504
BmiI GGNNCC 4 cut(s) 506, 858, 1069, 1233
BmrFI CCNGG 6 cut(s) 194, 195, 225, 956, 957, 1405
BmrI ACTGGG 1 cut(s) 894
BmsI GCATC 4 cut(s) 158, 651, 751, 1354
BmtI GCTAGC 3 cut(s) 57, 434, 1436
BmuI ACTGGG 1 cut(s) 894
BpiI GAAGAC 3 cut(s) 80, 759, 1488
BpuEI CTTGAG 1 cut(s) 1616
BpuMI CCSGG 5 cut(s) 194, 195, 225, 956, 957
BsaAI YACGTR 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 739
BsaI GGTCTC 1 cut(s) 1156
BsaJI CCNNGG 6 cut(s) 46, 193, 955, 956, 1116, 1548
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 1433, 1463
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 514, 706, 1117, 1276, 1297
Bse1I ACTGG 2 cut(s) 889, 1152
Bse3DI GCAATG 1 cut(s) 479
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 1 cut(s) 1405
BseDI CCNNGG 6 cut(s) 46, 193, 955, 956, 1116, 1548
BseGI GGATG 1 cut(s) 1369
BseLI CCNNNNNNNGG 6 cut(s) 16, 514, 706, 1117, 1276, 1297
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 3 cut(s) 809, 1155, 1592
BseNI ACTGG 2 cut(s) 889, 1152
BseRI GAGGAG 2 cut(s) 374, 617
BseXI GCAGC 2 cut(s) 207, 1507
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 3 cut(s) 45, 676, 1553
BsiHKCI CYCGRG 2 cut(s) 193, 955
BsiSI CCGG 4 cut(s) 92, 194, 225, 956
BslFI GGGAC 2 cut(s) 176, 490
BslI CCNNNNNNNGG 6 cut(s) 16, 514, 706, 1117, 1276, 1297
BsmAI GTCTC 2 cut(s) 1144, 1156
BsmFI GGGAC 2 cut(s) 176, 490
BsnI GGCC 3 cut(s) 45, 676, 1553
Bso31I GGTCTC 1 cut(s) 1156
BsoBI CYCGRG 2 cut(s) 193, 955
Bsp1286I GDGCHC 1 cut(s) 701
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
BspANI GGCC 3 cut(s) 45, 676, 1553
BspCNI CTCAG 3 cut(s) 810, 1154, 1591
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 4 cut(s) 506, 858, 1069, 1233
BspOI GCTAGC 3 cut(s) 57, 434, 1436
BspPI GGATC 4 cut(s) 144, 392, 1062, 1075
BspTI CTTAAG 1 cut(s) 1295
BspTNI GGTCTC 1 cut(s) 1156
BsrDI GCAATG 1 cut(s) 479
BsrI ACTGG 2 cut(s) 889, 1152
BssECI CCNNGG 6 cut(s) 46, 193, 955, 956, 1116, 1548
BssMI GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
BssNAI GTATAC 2 cut(s) 797, 1001
BssNI GRCGYC 1 cut(s) 739
BssSI CACGAG 2 cut(s) 897, 1239
BssT1I CCWWGG 2 cut(s) 46, 1548
Bst1107I GTATAC 2 cut(s) 797, 1001
Bst2BI CACGAG 2 cut(s) 897, 1239
Bst2UI CCWGG 1 cut(s) 1405
Bst4CI ACNGT 3 cut(s) 356, 665, 1033
Bst6I CTCTTC 2 cut(s) 408, 1161
BstACI GRCGYC 1 cut(s) 739
BstAFI CTTAAG 1 cut(s) 1295
BstBAI YACGTR 1 cut(s) 381
BstC8I GCNNGC 8 cut(s) 11, 55, 151, 349, 432, 832, 1077, 1434
BstDEI CTNAG 7 cut(s) 671, 767, 818, 1141, 1160, 1368, 1578
BstF5I GGATG 1 cut(s) 1369
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 220, 544
BstKTI GATC 7 cut(s) 139, 251, 378, 387, 424, 824, 1070
BstMAI GTCTC 2 cut(s) 1144, 1156
BstMBI GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
BstMWI GCNNNNNNNGC 2 cut(s) 297, 696
BstNI CCWGG 1 cut(s) 1405
BstNSI RCATGY 2 cut(s) 351, 834
BstSCI CCNGG 6 cut(s) 192, 193, 223, 954, 955, 1403
BstSFI CTRYAG 1 cut(s) 442
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 207, 1507
BstV2I GAAGAC 3 cut(s) 80, 759, 1488
BstX2I RGATCY 5 cut(s) 136, 384, 421, 821, 1067
BstYI RGATCY 5 cut(s) 136, 384, 421, 821, 1067
BstZ17I GTATAC 2 cut(s) 797, 1001
BsuI GTATCC 1 cut(s) 1461
BsuRI GGCC 3 cut(s) 45, 676, 1553
BtsCI GGATG 1 cut(s) 1369
BtsI GCAGTG 2 cut(s) 64, 160
BtsIMutI CAGTG 4 cut(s) 64, 160, 1038, 1145
Cac8I GCNNGC 8 cut(s) 11, 55, 151, 349, 432, 832, 1077, 1434
CfoI GCGC 2 cut(s) 220, 544
Cfr13I GGNCC 1 cut(s) 504
Cfr9I CCCGGG 2 cut(s) 193, 955
Csp6I GTAC 3 cut(s) 923, 1182, 1351
CviAII CATG 5 cut(s) 348, 633, 831, 1072, 1192
CviQI GTAC 3 cut(s) 923, 1182, 1351
DdeI CTNAG 7 cut(s) 671, 767, 818, 1141, 1160, 1368, 1578
DpnI GATC 7 cut(s) 138, 250, 377, 386, 423, 823, 1069
DpnII GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
DraI TTTAAA 2 cut(s) 616, 1188
Eam1104I CTCTTC 2 cut(s) 408, 1161
EarI CTCTTC 2 cut(s) 408, 1161
Eco130I CCWWGG 2 cut(s) 46, 1548
Eco147I AGGCCT 1 cut(s) 1553
Eco24I GRGCYC 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 1156
Eco32I GATATC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 504
Eco57I CTGAAG 3 cut(s) 410, 810, 1629
Eco72I CACGTG 1 cut(s) 381
Eco88I CYCGRG 2 cut(s) 193, 955
EcoO109I RGGNCCY 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 1403
EcoRV GATATC 1 cut(s) 624
EcoT14I CCWWGG 2 cut(s) 46, 1548
EcoT22I ATGCAT 2 cut(s) 133, 644
EcoT38I GRGCYC 1 cut(s) 701
ErhI CCWWGG 2 cut(s) 46, 1548
FaeI CATG 5 cut(s) 351, 636, 834, 1075, 1195
FalI AAGNNNNNCTT 4 cut(s) 1264, 1296, 1373, 1405
FaqI GGGAC 2 cut(s) 176, 490
FatI CATG 5 cut(s) 347, 632, 830, 1071, 1191
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 3 cut(s) 736, 796, 1000
Fnu4HI GCNGC 2 cut(s) 221, 1521
FokI GGATG 1 cut(s) 1376
FriOI GRGCYC 1 cut(s) 701
Fsp4HI GCNGC 2 cut(s) 221, 1521
FspBI CTAG 4 cut(s) 54, 431, 1212, 1433
GlaI GCGC 2 cut(s) 219, 543
GluI GCNGC 2 cut(s) 221, 1521
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 3 cut(s) 45, 676, 1553
HapII CCGG 4 cut(s) 92, 194, 225, 956
HhaI GCGC 2 cut(s) 220, 544
Hin1I GRCGYC 1 cut(s) 739
Hin1II CATG 5 cut(s) 351, 636, 834, 1075, 1195
Hin6I GCGC 2 cut(s) 218, 542
HinP1I GCGC 2 cut(s) 218, 542
HincII GTYRAC 2 cut(s) 654, 743
HindII GTYRAC 2 cut(s) 654, 743
HinfI GANTC 4 cut(s) 262, 283, 710, 1237
HpaI GTTAAC 1 cut(s) 654
HpaII CCGG 4 cut(s) 92, 194, 225, 956
HphI GGTGA 4 cut(s) 544, 791, 1091, 1608
Hpy166II GTNNAC 7 cut(s) 654, 737, 743, 797, 1001, 1477, 1616
Hpy188I TCNGA 5 cut(s) 438, 931, 1419, 1591, 1640
Hpy188III TCNNGA 2 cut(s) 92, 1580
Hpy8I GTNNAC 7 cut(s) 654, 737, 743, 797, 1001, 1477, 1616
HpyAV CCTTC 5 cut(s) 329, 493, 711, 1532, 1604
HpyCH4III ACNGT 3 cut(s) 356, 665, 1033
HpyCH4IV ACGT 2 cut(s) 380, 739
HpyCH4V TGCA 8 cut(s) 131, 171, 347, 351, 472, 480, 561, 642
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 696
HpyF3I CTNAG 7 cut(s) 671, 767, 818, 1141, 1160, 1368, 1578
HpySE526I ACGT 2 cut(s) 380, 739
Hsp92I GRCGYC 1 cut(s) 739
Hsp92II CATG 5 cut(s) 351, 636, 834, 1075, 1195
HspAI GCGC 2 cut(s) 218, 542
Kpn2I TCCGGA 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 375
KspAI GTTAAC 1 cut(s) 654
Kzo9I GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
LmnI GCTCC 2 cut(s) 361, 862
Lsp1109I GCAGC 2 cut(s) 207, 1507
LweI GCATC 4 cut(s) 158, 651, 751, 1354
MaeI CTAG 4 cut(s) 54, 431, 1212, 1433
MaeII ACGT 2 cut(s) 380, 739
MaeIII GTNAC 4 cut(s) 107, 340, 1033, 1097
MalI GATC 7 cut(s) 138, 250, 377, 386, 423, 823, 1069
MboI GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
MfeI CAATTG 2 cut(s) 1394, 1506
MflI RGATCY 5 cut(s) 136, 384, 421, 821, 1067
MhlI GDGCHC 1 cut(s) 701
MlyI GAGTC 1 cut(s) 1231
MmeI TCCRAC 1 cut(s) 1338
Mph1103I ATGCAT 2 cut(s) 133, 644
MroI TCCGGA 1 cut(s) 91
MroXI GAANNNNTTC 3 cut(s) 312, 714, 786
MslI CAYNNNNRTG 1 cut(s) 637
MspCI CTTAAG 1 cut(s) 1295
MspI CCGG 4 cut(s) 92, 194, 225, 956
MspR9I CCNGG 6 cut(s) 194, 195, 225, 956, 957, 1405
MunI CAATTG 2 cut(s) 1394, 1506
MvaI CCWGG 1 cut(s) 1405
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 297, 696
NciI CCSGG 5 cut(s) 194, 195, 225, 956, 957
NdeII GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
NheI GCTAGC 3 cut(s) 53, 430, 1432
NlaIII CATG 5 cut(s) 351, 636, 834, 1075, 1195
NlaIV GGNNCC 4 cut(s) 506, 858, 1069, 1233
NmuCI GTSAC 3 cut(s) 107, 1033, 1097
NsiI ATGCAT 2 cut(s) 133, 644
NspI RCATGY 2 cut(s) 351, 834
PaeI GCATGC 2 cut(s) 351, 834
PceI AGGCCT 1 cut(s) 1553
PdmI GAANNNNTTC 3 cut(s) 312, 714, 786
PfeI GAWTC 3 cut(s) 262, 283, 710
PflFI GACNNNGTC 1 cut(s) 1136
PflMI CCANNNNNTGG 1 cut(s) 1276
PkrI GCNGC 2 cut(s) 222, 1522
PleI GAGTC 1 cut(s) 1231
PmaCI CACGTG 1 cut(s) 381
PmlI CACGTG 1 cut(s) 381
PpsI GAGTC 1 cut(s) 1231
Ppu21I YACGTR 1 cut(s) 381
PpuMI RGGWCCY 1 cut(s) 504
PshBI ATTAAT 1 cut(s) 1491
Psp5II RGGWCCY 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 1403
PspCI CACGTG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 1403
PspN4I GGNNCC 4 cut(s) 506, 858, 1069, 1233
PspPI GGNCC 1 cut(s) 504
PspPPI RGGWCCY 1 cut(s) 504
PsuI RGATCY 5 cut(s) 136, 384, 421, 821, 1067
PsyI GACNNNGTC 1 cut(s) 1136
RsaI GTAC 3 cut(s) 924, 1183, 1352
RsaNI GTAC 3 cut(s) 923, 1182, 1351
RseI CAYNNNNRTG 1 cut(s) 637
SatI GCNGC 2 cut(s) 221, 1521
Sau3AI GATC 7 cut(s) 136, 248, 375, 384, 421, 821, 1067
Sau96I GGNCC 1 cut(s) 504
ScaI AGTACT 1 cut(s) 1183
SchI GAGTC 1 cut(s) 1231
ScrFI CCNGG 6 cut(s) 194, 195, 225, 956, 957, 1405
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 4 cut(s) 158, 651, 751, 1354
SfcI CTRYAG 1 cut(s) 442
SinI GGWCC 1 cut(s) 504
SmaI CCCGGG 2 cut(s) 195, 957
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 2 cut(s) 1295, 1595
SmoI CTYRAG 2 cut(s) 1295, 1595
SphI GCATGC 2 cut(s) 351, 834
SseBI AGGCCT 1 cut(s) 1553
SspI AATATT 1 cut(s) 872
SspMI CTAG 4 cut(s) 54, 431, 1212, 1433
StuI AGGCCT 1 cut(s) 1553
StyD4I CCNGG 6 cut(s) 192, 193, 223, 954, 955, 1403
StyI CCWWGG 2 cut(s) 46, 1548
TaaI ACNGT 3 cut(s) 356, 665, 1033
TaiI ACGT 2 cut(s) 383, 742
TaqI TCGA 4 cut(s) 145, 207, 370, 626
TatI WGTACW 1 cut(s) 1181
TfiI GAWTC 3 cut(s) 262, 283, 710
TscAI CASTG 4 cut(s) 64, 160, 1038, 1152
TseFI GTSAC 3 cut(s) 107, 1033, 1097
TseI GCWGC 2 cut(s) 220, 1520
Tsp45I GTSAC 3 cut(s) 107, 1033, 1097
TspDTI ATGAA 7 cut(s) 621, 707, 779, 831, 1010, 1208, 1347
TspGWI ACGGA 2 cut(s) 476, 1085
TspMI CCCGGG 2 cut(s) 193, 955
TspRI CASTG 4 cut(s) 64, 160, 1038, 1152
Tth111I GACNNNGTC 1 cut(s) 1136
Van91I CCANNNNNTGG 1 cut(s) 1276
Vha464I CTTAAG 1 cut(s) 1295
VpaK11BI GGWCC 1 cut(s) 504
VspI ATTAAT 1 cut(s) 1491
XapI RAATTY 4 cut(s) 202, 877, 917, 1055
XceI RCATGY 2 cut(s) 351, 834
XmaI CCCGGG 2 cut(s) 193, 955
XmiI GTMKAC 3 cut(s) 736, 796, 1000
XmnI GAANNNNTTC 3 cut(s) 312, 714, 786
XspI CTAG 4 cut(s) 54, 431, 1212, 1433
ZraI GACGTC 1 cut(s) 740
ZrmI AGTACT 1 cut(s) 1183
Zsp2I ATGCAT 2 cut(s) 133, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.