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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 98, 139 |
| AluBI | AGCT | 1 cut(s) | 157 |
| AluI | AGCT | 1 cut(s) | 157 |
| BciVI | GTATCC | 1 cut(s) | 19 |
| BfaI | CTAG | 1 cut(s) | 158 |
| BfuI | GTATCC | 1 cut(s) | 19 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 51, 81 |
| BseMII | CTCAG | 2 cut(s) | 66, 102 |
| BspCNI | CTCAG | 2 cut(s) | 67, 103 |
| Bst4CI | ACNGT | 1 cut(s) | 124 |
| BstDEI | CTNAG | 2 cut(s) | 75, 111 |
| BsuI | GTATCC | 1 cut(s) | 19 |
| BtsIMutI | CAGTG | 1 cut(s) | 129 |
| CviJI | RGCY | 1 cut(s) | 157 |
| CviKI_1 | RGCY | 1 cut(s) | 157 |
| DdeI | CTNAG | 2 cut(s) | 75, 111 |
| FaiI | YATR | 3 cut(s) | 34, 36, 50 |
| FauNDI | CATATG | 1 cut(s) | 34 |
| FspBI | CTAG | 1 cut(s) | 158 |
| FspEI | CC | 8 cut(s) | 8, 23, 35, 42, 53, 74, 118, 147 |
| Hpy188I | TCNGA | 1 cut(s) | 150 |
| HpyAV | CCTTC | 1 cut(s) | 155 |
| HpyCH4III | ACNGT | 1 cut(s) | 124 |
| HpyF3I | CTNAG | 2 cut(s) | 75, 111 |
| MaeI | CTAG | 1 cut(s) | 158 |
| MboII | GAAGA | 1 cut(s) | 53 |
| MluCI | AATT | 1 cut(s) | 93 |
| NdeI | CATATG | 1 cut(s) | 34 |
| SetI | ASST | 2 cut(s) | 137, 159 |
| Sse9I | AATT | 1 cut(s) | 93 |
| SspMI | CTAG | 1 cut(s) | 158 |
| TaaI | ACNGT | 1 cut(s) | 124 |
| TaqI | TCGA | 1 cut(s) | 102 |
| TasI | AATT | 1 cut(s) | 93 |
| TscAI | CASTG | 1 cut(s) | 129 |
| TspDTI | ATGAA | 2 cut(s) | 33, 78 |
| TspRI | CASTG | 1 cut(s) | 129 |
| XspI | CTAG | 1 cut(s) | 158 |