MD03G1256400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000270
Physical Location & Seq
Reverse (-)
34399545 .. 34400381
837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1256400.v1.1.491

Sequence Viewer

Length: 837 bp
ATGTATATTGACGATCTTCCTCATCATCTCTTGGTTGAAATTCTTTGTCGACTTCCATATAAAATTGTTATTCAAAGCAACTGCGTGTCCAAGCAATGGTTCTCTGTCATCTCCGATCCATCTTTTATTCACCGCTTTGTACGTCAACAAGGACATATCGATTCAGATACGATATTGTTAGATATCAGCACACAGGTCCTTAGGGATGTATTATTATATACCGTGAGGTCGATTTCGGACGAGTTCAAAACACGCGAGATTTCTTTCCCTTGGGGGCAATGTTCTCTAAGTTTATTCCCTTGGATGCAACGTGTGGTAGCGACGCATAACGATTTAATCTTGTGCTGTCGAGTCCGCTGCTTTGAGCGTAGATATGAGCGTGATTACTGTATCTGCAATCCGTACACCAAGCAGTCGATAAAGCTTCCTCCTCCCCCACGAGTTCATGACTTAACAGTACGCGCGGGGCTCATATGTGACAATCCTTACTATAACCCTACCGAAGATGGCTGCACTACGGTCAAATTTAATACCGAGTACAGGTACAGGGTTGTGCGAATCCCTCCAAAATGTGACGACCTAAGCTCATTCACAGTTGAGATGTTCTCGTCTGAGACTGGTGAATGGAGAGAGATAGTTATATTATGCTCCCCAGGCTCTAAGTTCGTTTGTGTCCATCACCCGTGTATTGCTTTAGATGGAATGTTGTATTGGATGGACCACAATGGCAGGCTTTTCGGGTTTGATCCATTCAACAACATGAACAGCACAATCCGTGCTACTGCTAAATTTGTTGACGACAACGAATTATGTTTATTTGGGAGTGGAAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

279

Amino Acids

32.37

Weight (kDa)

6.74

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 48 3.6e-07 F-box-like
F-box PF00646 3 - 43 2.7e-06 F-box domain
b-prop_At3g26010-like PF24750 106 - 242 4.1e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 96
AccI GTMKAC 1 cut(s) 49
AccII CGCG 3 cut(s) 255, 462, 464
AciI CCGC 3 cut(s) 133, 355, 464
AclWI GGATC 2 cut(s) 110, 740
AcsI RAATTY 3 cut(s) 39, 524, 788
AfaI GTAC 5 cut(s) 141, 404, 459, 539, 545
AfiI CCNNNNNNNGG 2 cut(s) 96, 540
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 38, 74, 247, 754
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 4 cut(s) 83, 115, 694, 726
AluBI AGCT 2 cut(s) 424, 585
AluI AGCT 2 cut(s) 424, 585
Alw26I GTCTC 1 cut(s) 608
AlwI GGATC 2 cut(s) 110, 740
ApeKI GCWGC 2 cut(s) 357, 510
ApoI RAATTY 3 cut(s) 39, 524, 788
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 2 cut(s) 196, 718
AsuHPI GGTGA 3 cut(s) 122, 632, 671
AvaII GGWCC 2 cut(s) 196, 718
AxyI CCTNAGG 1 cut(s) 200
BanII GRGCYC 1 cut(s) 471
BauI CACGAG 1 cut(s) 438
BbvI GCAGC 2 cut(s) 344, 497
BccI CCATC 5 cut(s) 127, 500, 684, 692, 709
BcgI CGANNNNNNTGC 4 cut(s) 339, 373, 718, 752
BciT130I CCWGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 608
BisI GCNGC 2 cut(s) 358, 511
BlsI GCNGC 2 cut(s) 359, 512
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 2 cut(s) 196, 718
BmgT120I GGNCC 2 cut(s) 196, 718
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 590, 622
Bpu10I CCTNAGC 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 269, 299, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 540
Bse1I ACTGG 1 cut(s) 622
Bse21I CCTNAGG 1 cut(s) 200
Bse3DI GCAATG 2 cut(s) 101, 284
BseBI CCWGG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 269, 299, 652
BseGI GGATG 3 cut(s) 211, 309, 720
BseLI CCNNNNNNNGG 2 cut(s) 96, 540
BseMI GCAATG 2 cut(s) 101, 284
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 622
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 2 cut(s) 344, 497
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 3 cut(s) 255, 462, 464
BshVI ATCGAT 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 96, 540
BsmAI GTCTC 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 3 cut(s) 13, 115, 745
BspACI CCGC 3 cut(s) 133, 355, 464
BspCNI CTCAG 1 cut(s) 604
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 3 cut(s) 255, 462, 464
BspHI TCATGA 1 cut(s) 445
BspPI GGATC 2 cut(s) 110, 740
BsrDI GCAATG 2 cut(s) 101, 284
BsrI ACTGG 1 cut(s) 622
BssECI CCNNGG 3 cut(s) 269, 299, 652
BssMI GATC 3 cut(s) 13, 115, 745
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 269, 299
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 5 cut(s) 223, 389, 457, 520, 595
BstC8I GCNNGC 1 cut(s) 731
BstDEI CTNAG 5 cut(s) 200, 287, 581, 612, 660
BstF5I GGATG 3 cut(s) 211, 309, 720
BstFNI CGCG 3 cut(s) 255, 462, 464
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 3 cut(s) 16, 118, 748
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 3 cut(s) 13, 115, 745
BstMWI GCNNNNNNNGC 1 cut(s) 654
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 652
BstUI CGCG 3 cut(s) 255, 462, 464
BstV1I GCAGC 2 cut(s) 344, 497
Bsu15I ATCGAT 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 3 cut(s) 211, 309, 720
Cac8I GCNNGC 1 cut(s) 731
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 2 cut(s) 196, 718
ClaI ATCGAT 1 cut(s) 159
CseI GACGC 1 cut(s) 331
Csp6I GTAC 5 cut(s) 140, 403, 458, 538, 544
CviAII CATG 2 cut(s) 446, 760
CviJI RGCY 6 cut(s) 424, 469, 510, 585, 657, 733
CviKI_1 RGCY 6 cut(s) 424, 469, 510, 585, 657, 733
CviQI GTAC 5 cut(s) 140, 403, 458, 538, 544
DdeI CTNAG 5 cut(s) 200, 287, 581, 612, 660
DpnI GATC 3 cut(s) 15, 117, 747
DpnII GATC 3 cut(s) 13, 115, 745
Eco130I CCWWGG 2 cut(s) 269, 299
Eco24I GRGCYC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 196, 718
Eco81I CCTNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 269, 299
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 2 cut(s) 269, 299
FaeI CATG 2 cut(s) 449, 763
FatI CATG 2 cut(s) 445, 759
FauI CCCGC 1 cut(s) 457
FauNDI CATATG 1 cut(s) 473
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 358, 511
FokI GGATG 3 cut(s) 218, 316, 727
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 2 cut(s) 358, 511
GlaI GCGC 1 cut(s) 463
GluI GCNGC 2 cut(s) 358, 511
HgaI GACGC 1 cut(s) 331
HhaI GCGC 1 cut(s) 464
Hin1II CATG 2 cut(s) 449, 763
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 3 cut(s) 50, 146, 796
HindII GTYRAC 3 cut(s) 50, 146, 796
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 3 cut(s) 161, 351, 558
HphI GGTGA 3 cut(s) 122, 632, 671
Hpy166II GTNNAC 4 cut(s) 50, 146, 405, 796
Hpy188I TCNGA 4 cut(s) 115, 166, 238, 613
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 4 cut(s) 50, 146, 405, 796
Hpy99I CGWCG 1 cut(s) 325
HpyCH4III ACNGT 5 cut(s) 223, 389, 457, 520, 595
HpyCH4IV ACGT 2 cut(s) 142, 310
HpyCH4V TGCA 3 cut(s) 307, 396, 513
HpyF10VI GCNNNNNNNGC 1 cut(s) 654
HpyF3I CTNAG 5 cut(s) 200, 287, 581, 612, 660
HpySE526I ACGT 2 cut(s) 142, 310
Hsp92II CATG 2 cut(s) 449, 763
HspAI GCGC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 13, 115, 745
LmnI GCTCC 1 cut(s) 653
LpnPI CCDG 7 cut(s) 179, 526, 532, 603, 639, 666, 715
Lsp1109I GCAGC 2 cut(s) 344, 497
LweI GCATC 1 cut(s) 294
MaeII ACGT 2 cut(s) 142, 310
MaeIII GTNAC 2 cut(s) 476, 572
MalI GATC 3 cut(s) 15, 117, 747
MboI GATC 3 cut(s) 13, 115, 745
MboII GAAGA 2 cut(s) 8, 515
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 5 cut(s) 39, 63, 524, 788, 806
MlyI GAGTC 1 cut(s) 360
MnlI CCTC 5 cut(s) 30, 219, 438, 441, 573
MseI TTAA 3 cut(s) 335, 452, 528
MspA1I CMGCKG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 654
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 3 cut(s) 255, 462, 464
MwoI GCNNNNNNNGC 1 cut(s) 654
NdeI CATATG 1 cut(s) 473
NdeII GATC 3 cut(s) 13, 115, 745
NlaIII CATG 2 cut(s) 449, 763
NmuCI GTSAC 2 cut(s) 476, 572
PagI TCATGA 1 cut(s) 445
PfeI GAWTC 2 cut(s) 161, 558
PflMI CCANNNNNTGG 1 cut(s) 96
PkrI GCNGC 2 cut(s) 359, 512
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 196
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspPI GGNCC 2 cut(s) 196, 718
PspPPI RGGWCCY 1 cut(s) 196
RsaI GTAC 5 cut(s) 141, 404, 459, 539, 545
RsaNI GTAC 5 cut(s) 140, 403, 458, 538, 544
SalI GTCGAC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 335, 452, 528
SatI GCNGC 2 cut(s) 358, 511
Sau3AI GATC 3 cut(s) 13, 115, 745
Sau96I GGNCC 2 cut(s) 196, 718
SchI GAGTC 1 cut(s) 360
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 471
SetI ASST 8 cut(s) 145, 198, 230, 313, 426, 545, 582, 587
SfaNI GCATC 1 cut(s) 294
SinI GGWCC 2 cut(s) 196, 718
Sse9I AATT 5 cut(s) 39, 63, 524, 788, 806
SsiI CCGC 3 cut(s) 133, 355, 464
StyD4I CCNGG 1 cut(s) 652
StyI CCWWGG 2 cut(s) 269, 299
TaaI ACNGT 5 cut(s) 223, 389, 457, 520, 595
TaiI ACGT 2 cut(s) 145, 313
TaqI TCGA 5 cut(s) 49, 159, 230, 349, 416
TasI AATT 5 cut(s) 39, 63, 524, 788, 806
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 2 cut(s) 161, 558
Tru1I TTAA 3 cut(s) 335, 452, 528
Tru9I TTAA 3 cut(s) 335, 452, 528
TseFI GTSAC 2 cut(s) 476, 572
TseI GCWGC 2 cut(s) 357, 510
Tsp45I GTSAC 2 cut(s) 476, 572
TspDTI ATGAA 2 cut(s) 434, 776
TspGWI ACGGA 2 cut(s) 390, 764
Van91I CCANNNNNTGG 1 cut(s) 96
VpaK11BI GGWCC 2 cut(s) 196, 718
XapI RAATTY 3 cut(s) 39, 524, 788
XmiI GTMKAC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.