MD10G1000600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000270
Physical Location & Seq
Forward (+)
144434 .. 145570
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1000600.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGTTTAGCACTCTAGTTGTGGGAACTGGAACCTTTTTTCCAAGCATTACGGAGGAGGATCTGACTCCGAATCTGCAGCTACCACTTATTCTGGAAGCAGAGACATTATTTGTGCTAGGGGCATGTAATGATTTGCTTTTGTGCTGCCCAGCAAAGGATGATCAGCGTGAGTTATACATCTGTAATCCATATACCAAGCAGTGGGTTGCTGTTCCTCCTCCCCCAGAAGTCCAATGCATATGCCCTCCCATCGGATTCATTTGTGAACCCTACTACAACAGCTCCTCCAAGAAAGACGACTTAGACGACAGTGTTAATGATCATCATTGCAAATCACAACACAAACTTAGTTGTACTAGTACCGCTACCACTTCGTATACTAATACTACCATCCAGCTGAATGCTGAGTACAGGTGCAGGGTTGTGCGAATACTTCAAGATGGCCATGTCGACTTGTTCTTTTCAGAGACTTGTTATTGGAGAGAGTCAGTTAATGTAGCATTCCCAAGGTGTTATACTGAGATCTGTAGTGGTGGTGTTGCTCACAATGGGAAGTTGTATTGGCACGGCTCTGATGAGTTTTCAACCACGGATGATTTTACAGCCTTTACTTTTGAGCTGGATCCATTCGGTACTACTACTGCTGATGGGTGTCTGCAGGTGTGCCCACGAGAGTTTACTGATAATTTCAGTGTCTGGGAGTTGGAAGAGGTTGCTGGAAGAGGAGATGATTTGAAGTGGCGTTTGGTAGTTGATGAAGTTTCATTGTATCAAATAGACTCCTATTCCAAACACCCTTTACTCCTAAACTATGGAGGCCAAAAGTTCATCAAAATTGAGCTAGGCTTCCACCCAAATTATAAGGATATCATCTATTTAGATGACTATAGTCAAGTTCTGATGTGCAACCTTAGCGCAAGAACGCTAGAGTTAGTTCCAGGGATTCCGAGTTTCGGCATATATTTGGATCAACAGGGTATCCACCCATTTGTGCTTCCATGGTGGCCAACACCAGTTCCCAAACTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

344

Amino Acids

38.92

Weight (kDa)

4.62

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 861
AarI CACCTGC 1 cut(s) 649
Acc36I ACCTGC 1 cut(s) 649
AccB7I CCANNNNNTGG 1 cut(s) 201
AccI GTMKAC 2 cut(s) 377, 450
AciI CCGC 1 cut(s) 363
AclWI GGATC 4 cut(s) 66, 617, 630, 975
AcoI YGGCCR 2 cut(s) 442, 1004
AfaI GTAC 4 cut(s) 355, 361, 410, 634
AfiI CCNNNNNNNGG 4 cut(s) 154, 201, 251, 953
AgsI TTSAA 3 cut(s) 437, 585, 736
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 1 cut(s) 937
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AloI GAACNNNNNNTCC 2 cut(s) 22, 54
AluBI AGCT 5 cut(s) 79, 282, 397, 619, 841
AluI AGCT 5 cut(s) 79, 282, 397, 619, 841
Alw26I GTCTC 2 cut(s) 95, 461
AlwI GGATC 4 cut(s) 66, 617, 630, 975
AlwNI CAGNNNCTG 1 cut(s) 696
AoxI GGCC 3 cut(s) 442, 817, 1004
ApeKI GCWGC 2 cut(s) 76, 144
AspLEI GCGC 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 668
BalI TGGCCA 2 cut(s) 444, 1006
BamHI GGATCC 1 cut(s) 622
BauI CACGAG 1 cut(s) 669
BbvI GCAGC 2 cut(s) 88, 131
BccI CCATC 4 cut(s) 257, 398, 434, 641
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 939
BciVI GTATCC 1 cut(s) 989
BclI TGATCA 2 cut(s) 160, 319
BcoDI GTCTC 2 cut(s) 95, 461
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 6 cut(s) 14, 116, 357, 842, 926, 1030
BfmI CTRYAG 4 cut(s) 74, 526, 656, 886
BfuAI ACCTGC 1 cut(s) 649
BfuI GTATCC 1 cut(s) 989
BglII AGATCT 1 cut(s) 522
BisI GCNGC 2 cut(s) 77, 145
BlsI GCNGC 2 cut(s) 78, 146
Bme1390I CCNGG 1 cut(s) 939
BmiI GGNNCC 2 cut(s) 31, 624
BmrFI CCNGG 1 cut(s) 939
BoxI GACNNNNGTC 1 cut(s) 888
Bpu10I CCTNAGC 1 cut(s) 911
BsaJI CCNNGG 4 cut(s) 506, 588, 938, 998
BsaXI ACNNNNNCTCC 4 cut(s) 266, 269, 296, 299
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 201, 251, 953
Bse1I ACTGG 2 cut(s) 31, 1013
Bse3DI GCAATG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 506, 588, 938, 998
BseGI GGATG 3 cut(s) 163, 390, 598
BseLI CCNNNNNNNGG 4 cut(s) 154, 201, 251, 953
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 396, 510
BseNI ACTGG 2 cut(s) 31, 1013
BseRI GAGGAG 4 cut(s) 68, 207, 274, 738
BseSI GKGCMC 1 cut(s) 668
BseXI GCAGC 2 cut(s) 88, 131
BseYI CCCAGC 1 cut(s) 148
BsgI GTGCAG 1 cut(s) 436
BshFI GGCC 3 cut(s) 444, 819, 1006
BslI CCNNNNNNNGG 4 cut(s) 154, 201, 251, 953
BsmAI GTCTC 2 cut(s) 95, 461
BsmI GAATGC 2 cut(s) 406, 500
BsnI GGCC 3 cut(s) 444, 819, 1006
Bsp1286I GDGCHC 1 cut(s) 668
Bsp143I GATC 6 cut(s) 58, 160, 319, 522, 622, 967
Bsp19I CCATGG 1 cut(s) 998
BspACI CCGC 1 cut(s) 363
BspANI GGCC 3 cut(s) 444, 819, 1006
BspCNI CTCAG 2 cut(s) 397, 511
BspLI GGNNCC 2 cut(s) 31, 624
BspMAI CTGCAG 2 cut(s) 78, 660
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 4 cut(s) 66, 617, 630, 975
BsrDI GCAATG 1 cut(s) 325
BsrI ACTGG 2 cut(s) 31, 1013
BssECI CCNNGG 4 cut(s) 506, 588, 938, 998
BssMI GATC 6 cut(s) 58, 160, 319, 522, 622, 967
BssNAI GTATAC 1 cut(s) 378
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 2 cut(s) 506, 998
Bst1107I GTATAC 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 1 cut(s) 939
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 2 cut(s) 702, 715
BstDEI CTNAG 5 cut(s) 301, 347, 405, 519, 911
BstDSI CCRYGG 2 cut(s) 588, 998
BstF5I GGATG 3 cut(s) 163, 390, 598
BstHHI GCGC 1 cut(s) 917
BstKTI GATC 6 cut(s) 61, 163, 322, 525, 625, 970
BstMAI GTCTC 2 cut(s) 95, 461
BstMBI GATC 6 cut(s) 58, 160, 319, 522, 622, 967
BstMWI GCNNNNNNNGC 1 cut(s) 912
BstNI CCWGG 1 cut(s) 939
BstNSI RCATGY 1 cut(s) 126
BstPAI GACNNNNGTC 1 cut(s) 888
BstSCI CCNGG 1 cut(s) 937
BstSFI CTRYAG 4 cut(s) 74, 526, 656, 886
BstSLI GKGCMC 1 cut(s) 668
BstV1I GCAGC 2 cut(s) 88, 131
BstX2I RGATCY 3 cut(s) 58, 522, 622
BstYI RGATCY 3 cut(s) 58, 522, 622
BstZ17I GTATAC 1 cut(s) 378
BsuI GTATCC 1 cut(s) 989
BsuRI GGCC 3 cut(s) 444, 819, 1006
BtgI CCRYGG 2 cut(s) 588, 998
BtsCI GGATG 3 cut(s) 163, 390, 598
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 3 cut(s) 206, 316, 697
BveI ACCTGC 1 cut(s) 649
CaiI CAGNNNCTG 1 cut(s) 696
CfoI GCGC 1 cut(s) 917
Csp6I GTAC 4 cut(s) 354, 360, 409, 633
CviAII CATG 3 cut(s) 123, 446, 999
CviQI GTAC 4 cut(s) 354, 360, 409, 633
DdeI CTNAG 5 cut(s) 301, 347, 405, 519, 911
DpnI GATC 6 cut(s) 60, 162, 321, 524, 624, 969
DpnII GATC 6 cut(s) 58, 160, 319, 522, 622, 967
EaeI YGGCCR 2 cut(s) 442, 1004
Eam1104I CTCTTC 2 cut(s) 702, 715
EarI CTCTTC 2 cut(s) 702, 715
Eco130I CCWWGG 2 cut(s) 506, 998
Eco32I GATATC 1 cut(s) 868
EcoRII CCWGG 1 cut(s) 937
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 506, 998
EcoT22I ATGCAT 1 cut(s) 239
ErhI CCWWGG 2 cut(s) 506, 998
FaeI CATG 3 cut(s) 126, 449, 1002
FatI CATG 3 cut(s) 122, 445, 998
FauNDI CATATG 1 cut(s) 239
FbaI TGATCA 2 cut(s) 160, 319
FblI GTMKAC 2 cut(s) 377, 450
Fnu4HI GCNGC 2 cut(s) 77, 145
FokI GGATG 3 cut(s) 170, 377, 605
Fsp4HI GCNGC 2 cut(s) 77, 145
FspBI CTAG 6 cut(s) 14, 116, 357, 842, 926, 1030
GlaI GCGC 1 cut(s) 916
GluI GCNGC 2 cut(s) 77, 145
GsaI CCCAGC 1 cut(s) 152
HaeIII GGCC 3 cut(s) 444, 819, 1006
HhaI GCGC 1 cut(s) 917
Hin1II CATG 3 cut(s) 126, 449, 1002
Hin6I GCGC 1 cut(s) 915
HinP1I GCGC 1 cut(s) 915
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 6 cut(s) 64, 70, 255, 485, 779, 943
Hpy166II GTNNAC 4 cut(s) 266, 378, 451, 678
Hpy188I TCNGA 7 cut(s) 63, 69, 254, 466, 574, 900, 948
Hpy188III TCNNGA 2 cut(s) 92, 437
Hpy8I GTNNAC 4 cut(s) 266, 378, 451, 678
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 6 cut(s) 76, 237, 330, 417, 658, 906
HpyF10VI GCNNNNNNNGC 1 cut(s) 912
HpyF3I CTNAG 5 cut(s) 301, 347, 405, 519, 911
Hsp92II CATG 3 cut(s) 126, 449, 1002
HspAI GCGC 1 cut(s) 915
Ksp22I TGATCA 2 cut(s) 160, 319
Kzo9I GATC 6 cut(s) 58, 160, 319, 522, 622, 967
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 2 cut(s) 88, 131
MaeI CTAG 6 cut(s) 14, 116, 357, 842, 926, 1030
MalI GATC 6 cut(s) 60, 162, 321, 524, 624, 969
MboI GATC 6 cut(s) 58, 160, 319, 522, 622, 967
MboII GAAGA 2 cut(s) 719, 732
MflI RGATCY 3 cut(s) 58, 522, 622
MhlI GDGCHC 1 cut(s) 668
MlsI TGGCCA 2 cut(s) 444, 1006
MluCI AATT 3 cut(s) 685, 834, 856
MluNI TGGCCA 2 cut(s) 444, 1006
MlyI GAGTC 3 cut(s) 58, 494, 773
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 9 cut(s) 46, 49, 225, 228, 255, 295, 703, 716, 809
Mox20I TGGCCA 2 cut(s) 444, 1006
Mph1103I ATGCAT 1 cut(s) 239
MscI TGGCCA 2 cut(s) 444, 1006
MseI TTAA 3 cut(s) 315, 492, 1026
Msp20I TGGCCA 2 cut(s) 444, 1006
MspA1I CMGCKG 1 cut(s) 397
MspR9I CCNGG 1 cut(s) 939
Mva1269I GAATGC 2 cut(s) 406, 500
MvaI CCWGG 1 cut(s) 939
MwoI GCNNNNNNNGC 1 cut(s) 912
NcoI CCATGG 1 cut(s) 998
NdeI CATATG 1 cut(s) 239
NdeII GATC 6 cut(s) 58, 160, 319, 522, 622, 967
NlaIII CATG 3 cut(s) 126, 449, 1002
NlaIV GGNNCC 2 cut(s) 31, 624
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 649
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 2 cut(s) 406, 500
PfeI GAWTC 3 cut(s) 70, 255, 943
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 2 cut(s) 78, 146
PleI GAGTC 3 cut(s) 58, 493, 773
PpsI GAGTC 3 cut(s) 58, 493, 773
PshAI GACNNNNGTC 1 cut(s) 888
PsiI TTATAA 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 937
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 1 cut(s) 937
PspN4I GGNNCC 2 cut(s) 31, 624
PstI CTGCAG 2 cut(s) 78, 660
PstNI CAGNNNCTG 1 cut(s) 696
PsuI RGATCY 3 cut(s) 58, 522, 622
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 4 cut(s) 355, 361, 410, 634
RsaNI GTAC 4 cut(s) 354, 360, 409, 633
SalI GTCGAC 1 cut(s) 449
SaqAI TTAA 3 cut(s) 315, 492, 1026
SatI GCNGC 2 cut(s) 77, 145
Sau3AI GATC 6 cut(s) 58, 160, 319, 522, 622, 967
SchI GAGTC 3 cut(s) 58, 494, 773
ScrFI CCNGG 1 cut(s) 939
SduI GDGCHC 1 cut(s) 668
SfcI CTRYAG 4 cut(s) 74, 526, 656, 886
SpeI ACTAGT 1 cut(s) 356
Sse9I AATT 3 cut(s) 685, 834, 856
SsiI CCGC 1 cut(s) 363
SspMI CTAG 6 cut(s) 14, 116, 357, 842, 926, 1030
StyD4I CCNGG 1 cut(s) 937
StyI CCWWGG 2 cut(s) 506, 998
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 1 cut(s) 450
TasI AATT 3 cut(s) 685, 834, 856
TatI WGTACW 2 cut(s) 353, 408
TfiI GAWTC 3 cut(s) 70, 255, 943
Tru1I TTAA 3 cut(s) 315, 492, 1026
Tru9I TTAA 3 cut(s) 315, 492, 1026
TscAI CASTG 3 cut(s) 206, 316, 697
TseI GCWGC 2 cut(s) 76, 144
TspDTI ATGAA 4 cut(s) 247, 753, 771, 817
TspGWI ACGGA 2 cut(s) 65, 605
TspRI CASTG 3 cut(s) 206, 316, 697
Van91I CCANNNNNTGG 1 cut(s) 201
XceI RCATGY 1 cut(s) 126
XmiI GTMKAC 2 cut(s) 377, 450
XspI CTAG 6 cut(s) 14, 116, 357, 842, 926, 1030
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.