pycom05g22060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000270
Physical Location & Seq
Reverse (-)
24596800 .. 24597579
780 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22060.1

Sequence Viewer

Length: 780 bp
ATGTGTGATCCATACTACAACTCCTCTTCTCGAGAAGACAGTAGCAGTACAAGTTTTGATATCATCCAGCTTAATACTGAGTATAGGTGGACGATTGTGCGAATGGTTCGGAATTTGTCAGATGCTGGCTACCATGTGGAGATGATCTCTTCTGAGACTATAGGTGAATGGAGAGAATTGGCTCTGTTATGCAGCCCACAACAGTTAAAGCACTTGCAAAATATTCATACTGATATTGAAGGTTTCATTTCTTATTCCGCTGTTGCTCACAATAGAAAGTTTTATTGGTTGCCTACTACTTCTGAATGTACTTTTGAGTTGGATCCATTCATTGCTAATAGTAATGGTGATAATGTTGCTAAATGTCATTTCATCGACGAGCCAGACGCTCTTTTTATTAGCGCAAGTAACTGGCATCTAGGTGTGTGCAAAGGGTGTCTGCGGATGTGCAAGGTGGGATTAGGTGCTGATGATCCCTTCAGTGTTTGGGAGCTGAAATTGCAAGAGGCGCCCGAGAGAGATTGGGAATTTGAATGGTTAGCCGATAGAATAACCCAGCCTTTCGATGATATTCCTTTGTTCTCTGAACAGGAAGACAGAAGCTGCACGGTTGAGATGCTAAGTTTCCACCCGAATAATCAAGATATCATTTATTTCAGATTTGATCAACACATTGTCACGGGCAACTTTCGCGAAGGAAAGTTAGAGTTAGATATAGCTGCAAAGACTCCGTTTGATCTCGGTTCTTGGAGAGAGGTCTGCATATTTGCGCTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

260

Amino Acids

29.96

Weight (kDa)

4.64

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccII CGCG 1 cut(s) 693
AciI CCGC 2 cut(s) 258, 442
AclWI GGATC 4 cut(s) 2, 317, 330, 467
AcsI RAATTY 2 cut(s) 112, 527
AcuI CTGAAG 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 2 cut(s) 49, 310
AgsI TTSAA 2 cut(s) 239, 533
AjuI GAANNNNNNNTTGG 2 cut(s) 268, 300
AluBI AGCT 4 cut(s) 70, 493, 603, 719
AluI AGCT 4 cut(s) 70, 493, 603, 719
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 4 cut(s) 2, 317, 330, 467
AlwNI CAGNNNCTG 2 cut(s) 125, 603
Ama87I CYCGRG 2 cut(s) 30, 512
ApeKI GCWGC 3 cut(s) 192, 603, 719
ApoI RAATTY 2 cut(s) 112, 527
AspLEI GCGC 3 cut(s) 404, 511, 772
AsuHPI GGTGA 2 cut(s) 176, 359
AvaI CYCGRG 2 cut(s) 30, 512
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 508
BbsI GAAGAC 2 cut(s) 42, 600
BbvI GCAGC 3 cut(s) 204, 590, 706
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 512
BisI GCNGC 3 cut(s) 193, 604, 720
BlsI GCNGC 3 cut(s) 194, 605, 721
BmeT110I CYCGRG 2 cut(s) 30, 512
BmiI GGNNCC 2 cut(s) 324, 510
BmsI GCATC 3 cut(s) 112, 424, 606
BpiI GAAGAC 2 cut(s) 42, 600
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BsaHI GRCGYC 1 cut(s) 509
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bse1I ACTGG 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 330
BseGI GGATG 2 cut(s) 63, 450
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 2 cut(s) 69, 144
BseNI ACTGG 1 cut(s) 416
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 3 cut(s) 204, 590, 706
BseYI CCCAGC 1 cut(s) 555
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 693
BshNI GGYRCC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 30, 512
BsmAI GTCTC 1 cut(s) 149
BsoBI CYCGRG 2 cut(s) 30, 512
Bsp143I GATC 6 cut(s) 7, 144, 322, 472, 664, 736
Bsp68I TCGCGA 1 cut(s) 693
BspACI CCGC 2 cut(s) 258, 442
BspCNI CTCAG 2 cut(s) 70, 145
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 2 cut(s) 324, 510
BspPI GGATC 4 cut(s) 2, 317, 330, 467
BspT107I GGYRCC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 1 cut(s) 416
BssMI GATC 6 cut(s) 7, 144, 322, 472, 664, 736
BssNI GRCGYC 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 41, 204, 610
Bst6I CTCTTC 2 cut(s) 31, 154
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 78, 153, 620
BstF5I GGATG 2 cut(s) 63, 450
BstFNI CGCG 1 cut(s) 693
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 3 cut(s) 404, 511, 772
BstKTI GATC 6 cut(s) 10, 147, 325, 475, 667, 739
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 6 cut(s) 7, 144, 322, 472, 664, 736
BstMWI GCNNNNNNNGC 3 cut(s) 499, 508, 690
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 3 cut(s) 204, 590, 706
BstV2I GAAGAC 2 cut(s) 42, 600
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BtsCI GGATG 2 cut(s) 63, 450
BtsIMutI CAGTG 1 cut(s) 487
BtuMI TCGCGA 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 127
CaiI CAGNNNCTG 2 cut(s) 125, 603
CfoI GCGC 3 cut(s) 404, 511, 772
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 48, 309
CviAII CATG 2 cut(s) 134, 777
CviQI GTAC 2 cut(s) 48, 309
DdeI CTNAG 3 cut(s) 78, 153, 620
DinI GGCGCC 1 cut(s) 510
DpnI GATC 6 cut(s) 9, 146, 324, 474, 666, 738
DpnII GATC 6 cut(s) 7, 144, 322, 472, 664, 736
Eam1104I CTCTTC 2 cut(s) 31, 154
EarI CTCTTC 2 cut(s) 31, 154
Eco32I GATATC 2 cut(s) 61, 646
Eco57I CTGAAG 1 cut(s) 463
Eco88I CYCGRG 2 cut(s) 30, 512
EcoRV GATATC 2 cut(s) 61, 646
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
FaeI CATG 2 cut(s) 137, 780
FaiI YATR 9 cut(s) 13, 84, 135, 161, 190, 228, 716, 764, 778
FatI CATG 2 cut(s) 133, 776
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 3 cut(s) 193, 604, 720
FokI GGATG 2 cut(s) 50, 457
Fsp4HI GCNGC 3 cut(s) 193, 604, 720
FspBI CTAG 1 cut(s) 419
GlaI GCGC 3 cut(s) 403, 510, 771
GluI GCNGC 3 cut(s) 193, 604, 720
GsaI CCCAGC 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 512
HgaI GACGC 1 cut(s) 395
HhaI GCGC 3 cut(s) 404, 511, 772
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 2 cut(s) 137, 780
Hin6I GCGC 3 cut(s) 402, 509, 770
HinP1I GCGC 3 cut(s) 402, 509, 770
HinfI GANTC 1 cut(s) 727
HphI GGTGA 2 cut(s) 176, 359
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 6 cut(s) 111, 121, 154, 304, 586, 659
Hpy188III TCNNGA 4 cut(s) 30, 32, 641, 692
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 3 cut(s) 233, 487, 689
HpyCH4III ACNGT 3 cut(s) 41, 204, 610
HpyCH4V TGCA 8 cut(s) 192, 217, 429, 450, 502, 606, 722, 762
HpyF10VI GCNNNNNNNGC 3 cut(s) 499, 508, 690
HpyF3I CTNAG 3 cut(s) 78, 153, 620
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 2 cut(s) 137, 780
HspAI GCGC 3 cut(s) 402, 509, 770
KasI GGCGCC 1 cut(s) 508
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 7, 144, 322, 472, 664, 736
LmnI GCTCC 2 cut(s) 490, 777
LpnPI CCDG 6 cut(s) 80, 111, 396, 397, 569, 575
Lsp1109I GCAGC 3 cut(s) 204, 590, 706
LweI GCATC 3 cut(s) 112, 424, 606
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 2 cut(s) 407, 676
MalI GATC 6 cut(s) 9, 146, 324, 474, 666, 738
MboI GATC 6 cut(s) 7, 144, 322, 472, 664, 736
MboII GAAGA 4 cut(s) 18, 47, 141, 605
MflI RGATCY 1 cut(s) 322
MluCI AATT 4 cut(s) 112, 176, 497, 527
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 1 cut(s) 721
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 3 cut(s) 34, 499, 748
MseI TTAA 2 cut(s) 72, 206
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 260
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 3 cut(s) 499, 508, 690
NarI GGCGCC 1 cut(s) 509
NdeII GATC 6 cut(s) 7, 144, 322, 472, 664, 736
NlaIII CATG 2 cut(s) 137, 780
NlaIV GGNNCC 2 cut(s) 324, 510
NmuCI GTSAC 1 cut(s) 676
NruI TCGCGA 1 cut(s) 693
PaeR7I CTCGAG 1 cut(s) 30
PkrI GCNGC 3 cut(s) 194, 605, 721
PleI GAGTC 1 cut(s) 721
PluTI GGCGCC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 721
PspFI CCCAGC 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 324, 510
PstNI CAGNNNCTG 2 cut(s) 125, 603
PsuI RGATCY 1 cut(s) 322
RruI TCGCGA 1 cut(s) 693
RsaI GTAC 2 cut(s) 49, 310
RsaNI GTAC 2 cut(s) 48, 309
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 2 cut(s) 72, 206
SatI GCNGC 3 cut(s) 193, 604, 720
Sau3AI GATC 6 cut(s) 7, 144, 322, 472, 664, 736
SchI GAGTC 1 cut(s) 721
SfaNI GCATC 3 cut(s) 112, 424, 606
SfcI CTRYAG 1 cut(s) 159
SfoI GGCGCC 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
Sse9I AATT 4 cut(s) 112, 176, 497, 527
SsiI CCGC 2 cut(s) 258, 442
SspDI GGCGCC 1 cut(s) 508
SspI AATATT 1 cut(s) 223
SspMI CTAG 1 cut(s) 419
TaaI ACNGT 3 cut(s) 41, 204, 610
TaqI TCGA 3 cut(s) 31, 375, 564
TasI AATT 4 cut(s) 112, 176, 497, 527
TatI WGTACW 2 cut(s) 47, 308
Tru1I TTAA 2 cut(s) 72, 206
Tru9I TTAA 2 cut(s) 72, 206
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 3 cut(s) 192, 603, 719
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 4 cut(s) 215, 235, 319, 361
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 1 cut(s) 487
XapI RAATTY 2 cut(s) 112, 527
XhoI CTCGAG 1 cut(s) 30
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.