RLG00000020531

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000270
Physical Location & Seq
Reverse (-)
66009208 .. 66011054
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020531

Sequence Viewer

Length: 1461 bp
ATGGGAAGACATTGTGGTACTACTGCTACAAAAAGATTACTTATTGTGCCATCTGAGTCTCAGTCCCACGTAAAAAAATCAAAATCATCACCGGAAACACCATGGCAAAGCAAGTTGAAGACTAGTGTTGATCATCTCCCTGACATGTTATTAGTTGAAATCCTTTGTCGGCTTCCTCACAATAAATTTGTCTTCCAATGCAAGTGCGTGTGCAAACGGTGGTCTTCTCTCATCTCTGATGAAAAGTTTCAGTGCCGTTTTCTATGGCTCCAAAGTCGTCAGGACCAAAAGCCAACGCCGGTGGGACGTACTATCATCTTCAGGGATGCCAAGGACATGAGGAAGTTTTTTATATTGCCGGAGCTCGAGCTTCCGATGTTCAAAACACACAACTTCTCTTTGAGTTTCCTCCGCTGTTTTGAACCTCCAGAGTTCATAGAAACAGATGATGATATTGATGATGGGCCAATTGTGGTCGGGACGTGCAATGACTTGGTTTTGTGCTGCGAAACCACAACTTTTCAAAGTTGTTACTACATTTGCAATCCATGCACCAAGCAATTTGTTCATCTTCCGCCCACCCCTCGACGCTACAAGGAAGTAAGAGTGGGATTCATCTGTGATCCCTATTATAAGGAAGATGGTGATGATCAGAAAGAAGAAAGTGATATCATCCAGGTTGATGCTGATTTCAGGTACGCTGTTGTGCGAATAGGTCCTTGCTCATCCCCATTCAAATTCAACCTTGAGATCTTCTCTTCAGAAACAGGTAAATGGACAGAATCGACTATAATGAGCACATGTAGCTTTCGTTTGGACAATCTGAATCGCAGCGGAGGCATTCCTTATAATGGAATGTTATATTGGTTGAATGCTGATTATCAAGATGCCTTCATTGTTGGGTTGGATCCCTACTATTACTCGAACAAAAACAACATTAATGATGAATGTCCTGGTTTTCATTTTATTGAATTGCCAGAAAGGGATGGAGAATTTCCCTTCTTAGGTGTCTGCGGAGGAGGTCGTCTGCGAATTTGCCATTTCTTTACTGATCAATTACGTGTCCCGATTCCCTTCTCTCATGAAACTGTTGACGTCCCTATGCCGTGTATGAGTGTGTGGGAATTGAAAGATGATGTTGATGACGATGTAGATGATTGGTTTAACTTGACAGGCATCATTGACCTGAGCACAATGGTTTTCGGAAATCCATTAACTTGTGAATGGGGAATCTACTCGAAGACCCCTTTGCTTGACTCAATCATGGTGCTGGCTTTCGATCCAAATAATGAAGATATACTTTATCTAGAATTGTTTGACCACATTGTCGCATGCAATATTTCTGAAAGAGAGATAAAGGGAACTACATTAACCATTCCATATAGAAGTACGGTTCGTTTTGGGAGAGGCGTCTTCCCATTTGTGATCCCAAAGTGGCCAACACCCCCTCCCGGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

487

Amino Acids

55.97

Weight (kDa)

5.11

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 44 - 81 5e-06 F-box-like
b-prop_At3g26010-like PF24750 158 - 396 1e-11 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 633, 849
AasI GACNNNNNNGTC 1 cut(s) 1325
AatII GACGTC 1 cut(s) 1098
AciI CCGC 4 cut(s) 412, 575, 834, 1014
AclWI GGATC 5 cut(s) 617, 902, 915, 1274, 1420
AcoI YGGCCR 1 cut(s) 1436
AcsI RAATTY 4 cut(s) 185, 737, 992, 1032
AcuI CTGAAG 2 cut(s) 304, 744
AcyI GRCGYC 2 cut(s) 1095, 1410
AfaI GTAC 4 cut(s) 19, 310, 698, 1390
AfiI CCNNNNNNNGG 3 cut(s) 851, 1004, 1451
AflIII ACRYGT 3 cut(s) 144, 800, 1060
AhlI ACTAGT 1 cut(s) 122
AjiI CACGTC 1 cut(s) 483
AjnI CCWGG 2 cut(s) 675, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 847, 879
AluBI AGCT 3 cut(s) 364, 370, 807
AluI AGCT 3 cut(s) 364, 370, 807
Alw21I GWGCWC 3 cut(s) 366, 800, 1193
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 5 cut(s) 617, 902, 915, 1274, 1420
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 2 cut(s) 464, 1436
ApeKI GCWGC 2 cut(s) 504, 831
ApoI RAATTY 4 cut(s) 185, 737, 992, 1032
AseI ATTAAT 1 cut(s) 939
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 3 cut(s) 283, 464, 716
AsuC2I CCSGG 1 cut(s) 1452
AsuHPI GGTGA 2 cut(s) 81, 656
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 2 cut(s) 283, 716
BalI TGGCCA 1 cut(s) 1438
BamHI GGATCC 1 cut(s) 907
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 6 cut(s) 13, 125, 184, 216, 1247, 1405
Bbv12I GWGCWC 3 cut(s) 366, 800, 1193
BbvI GCAGC 2 cut(s) 491, 843
BccI CCATC 4 cut(s) 58, 455, 635, 980
BceAI ACGGC 2 cut(s) 240, 1090
BciT130I CCWGG 2 cut(s) 677, 954
BclI TGATCA 3 cut(s) 130, 649, 1051
BcnI CCSGG 1 cut(s) 1452
BcoDI GTCTC 1 cut(s) 63
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 2 cut(s) 123, 1307
BglII AGATCT 1 cut(s) 750
BisI GCNGC 2 cut(s) 505, 832
BlsI GCNGC 2 cut(s) 506, 833
Bme1390I CCNGG 3 cut(s) 677, 954, 1452
Bme18I GGWCC 2 cut(s) 283, 716
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 483
BmgT120I GGNCC 3 cut(s) 283, 464, 716
BmiI GGNNCC 2 cut(s) 269, 909
BmrFI CCNGG 3 cut(s) 677, 954, 1452
BmsI GCATC 4 cut(s) 316, 673, 877, 1185
BpiI GAAGAC 6 cut(s) 13, 125, 184, 216, 1247, 1405
BplI GAGNNNNNCTC 2 cut(s) 740, 772
BpmI CTGGAG 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 1187
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 1 cut(s) 1452
BsaAI YACGTR 2 cut(s) 70, 1061
BsaHI GRCGYC 2 cut(s) 1095, 1410
BsaJI CCNNGG 2 cut(s) 101, 330
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 1 cut(s) 1432
Bsc4I CCNNNNNNNGG 3 cut(s) 851, 1004, 1451
Bse118I RCCGGY 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 677, 954
BseDI CCNNGG 2 cut(s) 101, 330
BseGI GGATG 4 cut(s) 331, 672, 725, 991
BseLI CCNNNNNNNGG 3 cut(s) 851, 1004, 1451
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 3 cut(s) 45, 74, 1178
BseRI GAGGAG 1 cut(s) 1032
BseXI GCAGC 2 cut(s) 491, 843
BshFI GGCC 2 cut(s) 466, 1438
BsiHKAI GWGCWC 3 cut(s) 366, 800, 1193
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 4 cut(s) 92, 299, 359, 1452
BslFI GGGAC 5 cut(s) 49, 318, 493, 1049, 1082
BslI CCNNNNNNNGG 3 cut(s) 851, 1004, 1451
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 5 cut(s) 49, 318, 493, 1049, 1082
BsmI GAATGC 2 cut(s) 840, 877
BsnI GGCC 2 cut(s) 466, 1438
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 366, 800, 1193
Bsp143I GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 4 cut(s) 412, 575, 834, 1014
BspANI GGCC 2 cut(s) 466, 1438
BspCNI CTCAG 3 cut(s) 46, 73, 1179
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 2 cut(s) 269, 909
BspPI GGATC 5 cut(s) 617, 902, 915, 1274, 1420
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 101, 330
BssMI GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
BssNI GRCGYC 2 cut(s) 1095, 1410
BssT1I CCWWGG 2 cut(s) 101, 330
Bst2UI CCWGG 2 cut(s) 677, 954
Bst4CI ACNGT 3 cut(s) 219, 1090, 1393
Bst6I CTCTTC 1 cut(s) 763
BstACI GRCGYC 2 cut(s) 1095, 1410
BstAPI GCANNNNNTGC 1 cut(s) 549
BstBAI YACGTR 2 cut(s) 70, 1061
BstC8I GCNNGC 2 cut(s) 1272, 1333
BstDEI CTNAG 4 cut(s) 54, 60, 1003, 1187
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 4 cut(s) 331, 672, 725, 991
BstKTI GATC 8 cut(s) 133, 625, 652, 753, 910, 1054, 1282, 1428
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
BstMWI GCNNNNNNNGC 3 cut(s) 549, 804, 837
BstNI CCWGG 2 cut(s) 677, 954
BstNSI RCATGY 3 cut(s) 148, 804, 1335
BstSCI CCNGG 3 cut(s) 675, 952, 1450
BstV1I GCAGC 2 cut(s) 491, 843
BstV2I GAAGAC 6 cut(s) 13, 125, 184, 216, 1247, 1405
BstX2I RGATCY 2 cut(s) 750, 907
BstYI RGATCY 2 cut(s) 750, 907
BsuRI GGCC 2 cut(s) 466, 1438
BtgI CCRYGG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 4 cut(s) 331, 672, 725, 991
BtsIMutI CAGTG 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 1272, 1333
CciI TCATGA 1 cut(s) 1081
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 3 cut(s) 283, 464, 716
CseI GACGC 2 cut(s) 597, 1399
Csp6I GTAC 4 cut(s) 18, 309, 697, 1389
CspCI CAANNNNNGTGG 2 cut(s) 282, 317
CviAII CATG 8 cut(s) 102, 145, 337, 549, 801, 1082, 1264, 1332
CviJI RGCY 9 cut(s) 172, 268, 292, 364, 370, 466, 807, 1274, 1438
CviKI_1 RGCY 9 cut(s) 172, 268, 292, 364, 370, 466, 807, 1274, 1438
CviQI GTAC 4 cut(s) 18, 309, 697, 1389
DdeI CTNAG 4 cut(s) 54, 60, 1003, 1187
DpnI GATC 8 cut(s) 132, 624, 651, 752, 909, 1053, 1281, 1427
DpnII GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
DrdI GACNNNNNNGTC 1 cut(s) 1325
DseDI GACNNNNNNGTC 1 cut(s) 1325
EaeI YGGCCR 1 cut(s) 1436
Eam1104I CTCTTC 1 cut(s) 763
EarI CTCTTC 1 cut(s) 763
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 101, 330
Eco24I GRGCYC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 2 cut(s) 283, 716
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 304, 744
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 675, 952
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 101, 330
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 101, 330
FaeI CATG 8 cut(s) 105, 148, 340, 552, 804, 1085, 1267, 1335
FalI AAGNNNNNCTT 2 cut(s) 1284, 1316
FaqI GGGAC 5 cut(s) 49, 318, 493, 1049, 1082
FatI CATG 8 cut(s) 101, 144, 336, 548, 800, 1081, 1263, 1331
FbaI TGATCA 3 cut(s) 130, 649, 1051
Fnu4HI GCNGC 2 cut(s) 505, 832
FokI GGATG 4 cut(s) 338, 659, 712, 998
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 2 cut(s) 505, 832
FspBI CTAG 2 cut(s) 123, 1307
GluI GCNGC 2 cut(s) 505, 832
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 2 cut(s) 466, 1438
HapII CCGG 4 cut(s) 92, 299, 359, 1452
HgaI GACGC 2 cut(s) 597, 1399
Hin1I GRCGYC 2 cut(s) 1095, 1410
Hin1II CATG 8 cut(s) 105, 148, 340, 552, 804, 1085, 1267, 1335
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HinfI GANTC 7 cut(s) 56, 612, 782, 826, 1069, 1230, 1256
HpaII CCGG 4 cut(s) 92, 299, 359, 1452
HphI GGTGA 2 cut(s) 81, 656
Hpy166II GTNNAC 1 cut(s) 1093
Hpy188I TCNGA 8 cut(s) 55, 238, 375, 654, 763, 825, 1205, 1345
Hpy188III TCNNGA 7 cut(s) 281, 428, 478, 884, 1066, 1082, 1307
Hpy8I GTNNAC 1 cut(s) 1093
Hpy99I CGWCG 1 cut(s) 591
HpyAV CCTTC 3 cut(s) 901, 1009, 1084
HpyCH4III ACNGT 3 cut(s) 219, 1090, 1393
HpyCH4IV ACGT 5 cut(s) 69, 307, 482, 1060, 1095
HpyCH4V TGCA 6 cut(s) 201, 213, 486, 543, 552, 1335
HpyF10VI GCNNNNNNNGC 3 cut(s) 549, 804, 837
HpyF3I CTNAG 4 cut(s) 54, 60, 1003, 1187
HpySE526I ACGT 5 cut(s) 69, 307, 482, 1060, 1095
Hsp92I GRCGYC 2 cut(s) 1095, 1410
Hsp92II CATG 8 cut(s) 105, 148, 340, 552, 804, 1085, 1267, 1335
Ksp22I TGATCA 3 cut(s) 130, 649, 1051
Kzo9I GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
LmnI GCTCC 2 cut(s) 273, 361
Lsp1109I GCAGC 2 cut(s) 491, 843
LweI GCATC 4 cut(s) 316, 673, 877, 1185
MaeI CTAG 2 cut(s) 123, 1307
MaeII ACGT 5 cut(s) 69, 307, 482, 1060, 1095
MaeIII GTNAC 1 cut(s) 530
MalI GATC 8 cut(s) 132, 624, 651, 752, 909, 1053, 1281, 1427
MboI GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 2 cut(s) 750, 907
MhlI GDGCHC 3 cut(s) 366, 800, 1193
MlsI TGGCCA 1 cut(s) 1438
MluNI TGGCCA 1 cut(s) 1438
MlyI GAGTC 2 cut(s) 65, 1250
MmeI TCCRAC 1 cut(s) 885
Mox20I TGGCCA 1 cut(s) 1438
MroXI GAANNNNTTC 1 cut(s) 246
MscI TGGCCA 1 cut(s) 1438
MseI TTAA 4 cut(s) 939, 1164, 1214, 1370
Msp20I TGGCCA 1 cut(s) 1438
MspA1I CMGCKG 2 cut(s) 414, 834
MspI CCGG 4 cut(s) 92, 299, 359, 1452
MspR9I CCNGG 3 cut(s) 677, 954, 1452
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 2 cut(s) 840, 877
MvaI CCWGG 2 cut(s) 677, 954
MwoI GCNNNNNNNGC 3 cut(s) 549, 804, 837
NciI CCSGG 1 cut(s) 1452
NcoI CCATGG 1 cut(s) 101
NdeII GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
NlaIII CATG 8 cut(s) 105, 148, 340, 552, 804, 1085, 1267, 1335
NlaIV GGNNCC 2 cut(s) 269, 909
NspI RCATGY 3 cut(s) 148, 804, 1335
PaeI GCATGC 1 cut(s) 1335
PaeR7I CTCGAG 1 cut(s) 365
PagI TCATGA 1 cut(s) 1081
PciI ACATGT 2 cut(s) 144, 800
PctI GAATGC 2 cut(s) 840, 877
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 5 cut(s) 612, 782, 826, 1069, 1230
PfoI TCCNGGA 1 cut(s) 1450
PkrI GCNGC 2 cut(s) 506, 833
PleI GAGTC 2 cut(s) 64, 1250
PpsI GAGTC 2 cut(s) 64, 1250
Ppu21I YACGTR 2 cut(s) 70, 1061
PpuMI RGGWCCY 1 cut(s) 716
PscI ACATGT 2 cut(s) 144, 800
PshBI ATTAAT 1 cut(s) 939
PsiI TTATAA 2 cut(s) 633, 849
Psp124BI GAGCTC 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 675, 952
PspGI CCWGG 2 cut(s) 675, 952
PspN4I GGNNCC 2 cut(s) 269, 909
PspPI GGNCC 3 cut(s) 283, 464, 716
PspPPI RGGWCCY 1 cut(s) 716
PspXI VCTCGAGB 1 cut(s) 365
PsuI RGATCY 2 cut(s) 750, 907
RsaI GTAC 4 cut(s) 19, 310, 698, 1390
RsaNI GTAC 4 cut(s) 18, 309, 697, 1389
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 4 cut(s) 939, 1164, 1214, 1370
SatI GCNGC 2 cut(s) 505, 832
Sau3AI GATC 8 cut(s) 130, 622, 649, 750, 907, 1051, 1279, 1425
Sau96I GGNCC 3 cut(s) 283, 464, 716
SchI GAGTC 2 cut(s) 65, 1250
ScrFI CCNGG 3 cut(s) 677, 954, 1452
SduI GDGCHC 3 cut(s) 366, 800, 1193
SfaNI GCATC 4 cut(s) 316, 673, 877, 1185
Sfr274I CTCGAG 1 cut(s) 365
SgrAI CRCCGGYG 1 cut(s) 298
SinI GGWCC 2 cut(s) 283, 716
SlaI CTCGAG 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 365, 746
SmoI CTYRAG 2 cut(s) 365, 746
SpeI ACTAGT 1 cut(s) 122
SphI GCATGC 1 cut(s) 1335
SsiI CCGC 4 cut(s) 412, 575, 834, 1014
SspI AATATT 1 cut(s) 1339
SspMI CTAG 2 cut(s) 123, 1307
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 3 cut(s) 675, 952, 1450
StyI CCWWGG 2 cut(s) 101, 330
TaaI ACNGT 3 cut(s) 219, 1090, 1393
TaiI ACGT 5 cut(s) 72, 310, 485, 1063, 1098
TaqI TCGA 6 cut(s) 366, 586, 785, 923, 1238, 1278
TfiI GAWTC 5 cut(s) 612, 782, 826, 1069, 1230
Tru1I TTAA 4 cut(s) 939, 1164, 1214, 1370
Tru9I TTAA 4 cut(s) 939, 1164, 1214, 1370
TscAI CASTG 1 cut(s) 257
TseI GCWGC 2 cut(s) 504, 831
TspDTI ATGAA 9 cut(s) 255, 424, 557, 604, 883, 950, 960, 1098, 1305
TspRI CASTG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 283, 716
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 4 cut(s) 185, 737, 992, 1032
XbaI TCTAGA 1 cut(s) 1306
XceI RCATGY 3 cut(s) 148, 804, 1335
XhoI CTCGAG 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 2 cut(s) 123, 1307
ZraI GACGTC 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.