Rmu_co8467147.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8467147.1
OG0000270
Physical Location & Seq
Reverse (-)
2 .. 1491
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8467147.1_g000001.1.cds

Sequence Viewer

Length: 1490 bp
atgtctacgaagaaagctttcgccgcagggatgtctagggatggcgatgctaccagaagcagccgccaaagaggtactactaccttagctctctctctcgttaaaaagtccaagagatatataataaattcaccaccaccaccatcgacaaccacgatttgtgatctatctgactttatagttgtcgaaatactttgtcgacttccttacaatattaaggtcgtatttcaatgcaagtgtgtgtccaagcgatggtttgctctcatctctggtaattattttgttagccgttttttatgtcaacatgattgccaaacaaagccttttgtgacttctcatgttataagcccctatacggattacagacttataccccttacctcgtcttccaaacttccaagcattttgtcaatgaatttccttccaccttttcgagctgatgagcaacgacaattactatataatgaaccaattgtggaaggaactttcaatgacttggtcttgtgctgcgcaaccgagcattatcaacgtgactactacatctgcaatccactcacccagcaatgggttgctctccctcccgcccctcgagctccctattcgaaagtgccggtgggattcatttgtgatccctactatgatcatggaattagtaccggaactagtagtagctcaatgatgaagcttaatactggatataggtgcagggttgtgcgcatcctcccagatgaaagatttcaaccaggactgggtttttatgtgcagatcttctcttctgagacaggtgaatggagagagtatcaattacctaatctaacagagttagaaaagcttggttggttcattaaatggcagcctggcgttgctttcaatggcaagttatattggtacaatgatggtggctttacttttgagttggatccgttcaatattagtagtggtggtgatattattaataacaagcgtcgtttcaatgtacgacggagactatgcttccatttatgtgtgtgccaagggcgtttgcgaatgtatccattctccatagcgggatatattagcatttgggaattgaatgaaaaatccggcaaatcggaatggtgcttggtatttgacaaagtttctctgttggatatggtttccaaagatcctaagatcactaaagaaaaatcacgcaaattggacgtgttaggtttcctcccaagcaatgaagatatcatatatttgaaatttcctgaacagatagtgatgtgcaatcttcgtgaaaacactttagagaagagtccggttgatccacctataaacattcgatttggtttcagttgctatgttagcgatcagtttgcagttgatcgctctacaaccatctatccatatgtgctcccgtgttggcctacaccagttcctagaatcaataaaccaaaggtttgccaggtcaaggtcttgaaacaatttgagatattcctggtgcagcatgctgcgcagcaggagtc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

497

Amino Acids

57.16

Weight (kDa)

8.98

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
Acc16I TGCGCA 3 cut(s) 511, 716, 1479
AccB7I CCANNNNNTGG 1 cut(s) 252
AccI GTMKAC 2 cut(s) 5, 199
AciI CCGC 4 cut(s) 24, 64, 582, 1046
AclWI GGATC 5 cut(s) 623, 914, 927, 1139, 1283
AcsI RAATTY 3 cut(s) 127, 415, 1226
AfaI GTAC 4 cut(s) 76, 655, 890, 978
AfiI CCNNNNNNNGG 6 cut(s) 252, 355, 564, 565, 749, 1435
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 9 cut(s) 230, 490, 740, 871, 928, 973, 1072, 1225, 1444
AhlI ACTAGT 1 cut(s) 662
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 4 cut(s) 742, 856, 1428, 1461
AluBI AGCT 7 cut(s) 17, 89, 437, 593, 672, 685, 832
AluI AGCT 7 cut(s) 17, 89, 437, 593, 672, 685, 832
Alw21I GWGCWC 2 cut(s) 595, 1380
Alw26I GTCTC 2 cut(s) 773, 979
AlwI GGATC 5 cut(s) 623, 914, 927, 1139, 1283
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 1 cut(s) 1388
ApeKI GCWGC 6 cut(s) 60, 507, 853, 1468, 1475, 1480
ApoI RAATTY 3 cut(s) 127, 415, 1226
AseI ATTAAT 1 cut(s) 954
Asp700I GAANNNNTTC 2 cut(s) 17, 735
AspLEI GCGC 3 cut(s) 512, 717, 1480
AsuHPI GGTGA 4 cut(s) 123, 547, 797, 956
AsuII TTCGAA 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 588
BamHI GGATCC 1 cut(s) 919
BanII GRGCYC 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 2 cut(s) 595, 1380
BbvI GCAGC 5 cut(s) 72, 494, 865, 1462, 1480
BccI CCATC 5 cut(s) 35, 151, 246, 890, 1370
BceAI ACGGC 1 cut(s) 273
BcgI CGANNNNNNTGC 2 cut(s) 1322, 1356
BciT130I CCWGG 4 cut(s) 744, 858, 1430, 1463
BciVI GTATCC 1 cut(s) 1041
BclI TGATCA 1 cut(s) 640
BcoDI GTCTC 2 cut(s) 773, 979
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 3 cut(s) 36, 663, 1404
BfuI GTATCC 1 cut(s) 1041
BglII AGATCT 1 cut(s) 765
BisI GCNGC 8 cut(s) 24, 61, 64, 508, 854, 1469, 1476, 1481
BlsI GCNGC 8 cut(s) 25, 62, 65, 509, 855, 1470, 1477, 1482
Bme1390I CCNGG 4 cut(s) 744, 858, 1430, 1463
BmeT110I CYCGRG 1 cut(s) 588
BmgBI CACGTC 1 cut(s) 1183
BmiI GGNNCC 1 cut(s) 921
BmrFI CCNGG 4 cut(s) 744, 858, 1430, 1463
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 2 cut(s) 37, 726
BmuI ACTGGG 1 cut(s) 758
BpiI GAAGAC 1 cut(s) 378
Bpu10I CCTNAGC 1 cut(s) 85
Bpu14I TTCGAA 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 1012
BsaWI WCCGGW 2 cut(s) 656, 1282
Bsc4I CCNNNNNNNGG 6 cut(s) 252, 355, 564, 565, 749, 1435
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 3 cut(s) 697, 753, 1397
Bse3DI GCAATG 2 cut(s) 569, 1210
BseBI CCWGG 4 cut(s) 744, 858, 1430, 1463
BseDI CCNNGG 1 cut(s) 1012
BseGI GGATG 3 cut(s) 36, 46, 717
BseLI CCNNNNNNNGG 6 cut(s) 252, 355, 564, 565, 749, 1435
BseMI GCAATG 2 cut(s) 569, 1210
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 3 cut(s) 697, 753, 1397
BseXI GCAGC 5 cut(s) 72, 494, 865, 1462, 1480
BseYI CCCAGC 1 cut(s) 558
BsgI GTGCAG 3 cut(s) 724, 782, 1487
BshFI GGCC 1 cut(s) 1390
BsiHKAI GWGCWC 2 cut(s) 595, 1380
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 4 cut(s) 611, 657, 1083, 1283
BslI CCNNNNNNNGG 6 cut(s) 252, 355, 564, 565, 749, 1435
BsmAI GTCTC 2 cut(s) 773, 979
BsnI GGCC 1 cut(s) 1390
BsoBI CYCGRG 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 595, 1380
BspACI CCGC 4 cut(s) 24, 64, 582, 1046
BspANI GGCC 1 cut(s) 1390
BspCNI CTCAG 1 cut(s) 769
BspLI GGNNCC 1 cut(s) 921
BspPI GGATC 5 cut(s) 623, 914, 927, 1139, 1283
BspT104I TTCGAA 1 cut(s) 602
BsrDI GCAATG 2 cut(s) 569, 1210
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 3 cut(s) 697, 753, 1397
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 1012
BssT1I CCWWGG 1 cut(s) 1012
Bst2UI CCWGG 4 cut(s) 744, 858, 1430, 1463
Bst6I CTCTTC 2 cut(s) 778, 1271
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 1473
BstDEI CTNAG 3 cut(s) 85, 777, 1149
BstF5I GGATG 3 cut(s) 36, 46, 717
BstHHI GCGC 3 cut(s) 512, 717, 1480
BstMAI GTCTC 2 cut(s) 773, 979
BstMWI GCNNNNNNNGC 4 cut(s) 23, 590, 1329, 1477
BstNI CCWGG 4 cut(s) 744, 858, 1430, 1463
BstNSI RCATGY 1 cut(s) 1475
BstSCI CCNGG 4 cut(s) 742, 856, 1428, 1461
BstV1I GCAGC 5 cut(s) 72, 494, 865, 1462, 1480
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 3 cut(s) 765, 919, 1144
BstYI RGATCY 3 cut(s) 765, 919, 1144
BsuI GTATCC 1 cut(s) 1041
BsuRI GGCC 1 cut(s) 1390
BtgZI GCGATG 2 cut(s) 60, 265
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 3 cut(s) 36, 46, 717
Cac8I GCNNGC 1 cut(s) 1473
CfoI GCGC 3 cut(s) 512, 717, 1480
Cfr10I RCCGGY 1 cut(s) 610
CseI GACGC 1 cut(s) 953
Csp6I GTAC 4 cut(s) 75, 654, 889, 977
CspCI CAANNNNNGTGG 2 cut(s) 880, 915
CviAII CATG 4 cut(s) 305, 338, 644, 1472
CviQI GTAC 4 cut(s) 75, 654, 889, 977
DdeI CTNAG 3 cut(s) 85, 777, 1149
Eam1104I CTCTTC 2 cut(s) 778, 1271
EarI CTCTTC 2 cut(s) 778, 1271
Ecl136II GAGCTC 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 1012
Eco24I GRGCYC 1 cut(s) 595
Eco32I GATATC 1 cut(s) 1213
Eco53kI GAGCTC 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 593
EcoRII CCWGG 4 cut(s) 742, 856, 1428, 1461
EcoRV GATATC 1 cut(s) 1213
EcoT14I CCWWGG 1 cut(s) 1012
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 1012
FaeI CATG 4 cut(s) 308, 341, 647, 1475
FatI CATG 4 cut(s) 304, 337, 643, 1471
FauI CCCGC 2 cut(s) 589, 1039
FauNDI CATATG 1 cut(s) 1372
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 2 cut(s) 5, 199
Fnu4HI GCNGC 8 cut(s) 24, 61, 64, 508, 854, 1469, 1476, 1481
FokI GGATG 3 cut(s) 43, 53, 704
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 8 cut(s) 24, 61, 64, 508, 854, 1469, 1476, 1481
FspAI RTGCGCAY 1 cut(s) 716
FspBI CTAG 3 cut(s) 36, 663, 1404
FspI TGCGCA 3 cut(s) 511, 716, 1479
GlaI GCGC 3 cut(s) 511, 716, 1479
GluI GCNGC 8 cut(s) 24, 61, 64, 508, 854, 1469, 1476, 1481
GsaI CCCAGC 1 cut(s) 562
HaeIII GGCC 1 cut(s) 1390
HapII CCGG 4 cut(s) 611, 657, 1083, 1283
HgaI GACGC 1 cut(s) 953
HhaI GCGC 3 cut(s) 512, 717, 1480
Hin1II CATG 4 cut(s) 308, 341, 647, 1475
Hin6I GCGC 3 cut(s) 510, 715, 1478
HinP1I GCGC 3 cut(s) 510, 715, 1478
HincII GTYRAC 2 cut(s) 200, 302
HindII GTYRAC 2 cut(s) 200, 302
HindIII AAGCTT 3 cut(s) 15, 683, 830
HinfI GANTC 4 cut(s) 618, 1279, 1407, 1487
HpaII CCGG 4 cut(s) 611, 657, 1083, 1283
HphI GGTGA 4 cut(s) 123, 547, 797, 956
Hpy166II GTNNAC 3 cut(s) 6, 200, 302
Hpy188I TCNGA 3 cut(s) 172, 778, 1093
Hpy188III TCNNGA 3 cut(s) 1232, 1259, 1441
Hpy8I GTNNAC 3 cut(s) 6, 200, 302
Hpy99I CGWCG 2 cut(s) 969, 984
HpyAV CCTTC 2 cut(s) 431, 473
HpyCH4IV ACGT 2 cut(s) 529, 1182
HpyCH4V TGCA 7 cut(s) 234, 546, 705, 763, 1251, 1343, 1468
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 590, 1329, 1477
HpyF3I CTNAG 3 cut(s) 85, 777, 1149
HpySE526I ACGT 2 cut(s) 529, 1182
Hsp92II CATG 4 cut(s) 308, 341, 647, 1475
HspAI GCGC 3 cut(s) 510, 715, 1478
Ksp22I TGATCA 1 cut(s) 640
LmnI GCTCC 2 cut(s) 598, 1383
Lsp1109I GCAGC 5 cut(s) 72, 494, 865, 1462, 1480
LweI GCATC 2 cut(s) 37, 726
MaeI CTAG 3 cut(s) 36, 663, 1404
MaeII ACGT 2 cut(s) 529, 1182
MaeIII GTNAC 2 cut(s) 328, 530
MboII GAAGA 7 cut(s) 22, 378, 760, 765, 1220, 1247, 1288
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 3 cut(s) 765, 919, 1144
MhlI GDGCHC 2 cut(s) 595, 1380
MlyI GAGTC 1 cut(s) 1288
MmeI TCCRAC 2 cut(s) 897, 1107
MnlI CCTC 6 cut(s) 65, 391, 588, 597, 731, 1205
MroXI GAANNNNTTC 2 cut(s) 17, 735
MseI TTAA 5 cut(s) 102, 216, 687, 846, 954
MslI CAYNNNNRTG 1 cut(s) 1002
MspI CCGG 4 cut(s) 611, 657, 1083, 1283
MspR9I CCNGG 4 cut(s) 744, 858, 1430, 1463
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 4 cut(s) 744, 858, 1430, 1463
MwoI GCNNNNNNNGC 4 cut(s) 23, 590, 1329, 1477
NdeI CATATG 1 cut(s) 1372
NlaIII CATG 4 cut(s) 308, 341, 647, 1475
NlaIV GGNNCC 1 cut(s) 921
NmuCI GTSAC 2 cut(s) 328, 530
NsbI TGCGCA 3 cut(s) 511, 716, 1479
NspI RCATGY 1 cut(s) 1475
NspV TTCGAA 1 cut(s) 602
PaeI GCATGC 1 cut(s) 1475
PaeR7I CTCGAG 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 152
PdmI GAANNNNTTC 2 cut(s) 17, 735
PfeI GAWTC 2 cut(s) 618, 1407
PflFI GACNNNGTC 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 252
PkrI GCNGC 8 cut(s) 25, 62, 65, 509, 855, 1470, 1477, 1482
PleI GAGTC 1 cut(s) 1287
PpsI GAGTC 1 cut(s) 1287
PshBI ATTAAT 1 cut(s) 954
PsiI TTATAA 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 595
Psp6I CCWGG 4 cut(s) 742, 856, 1428, 1461
PspFI CCCAGC 1 cut(s) 558
PspGI CCWGG 4 cut(s) 742, 856, 1428, 1461
PspN4I GGNNCC 1 cut(s) 921
PspXI VCTCGAGB 1 cut(s) 588
PsuI RGATCY 3 cut(s) 765, 919, 1144
PsyI GACNNNGTC 1 cut(s) 497
RsaI GTAC 4 cut(s) 76, 655, 890, 978
RsaNI GTAC 4 cut(s) 75, 654, 889, 977
RseI CAYNNNNRTG 1 cut(s) 1002
SacI GAGCTC 1 cut(s) 595
SalI GTCGAC 1 cut(s) 198
SaqAI TTAA 5 cut(s) 102, 216, 687, 846, 954
SatI GCNGC 8 cut(s) 24, 61, 64, 508, 854, 1469, 1476, 1481
SchI GAGTC 1 cut(s) 1288
ScrFI CCNGG 4 cut(s) 744, 858, 1430, 1463
SduI GDGCHC 2 cut(s) 595, 1380
SfaNI GCATC 2 cut(s) 37, 726
Sfr274I CTCGAG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 602
SlaI CTCGAG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
SpeI ACTAGT 1 cut(s) 662
SphI GCATGC 1 cut(s) 1475
SsiI CCGC 4 cut(s) 24, 64, 582, 1046
SspI AATATT 2 cut(s) 214, 931
SspMI CTAG 3 cut(s) 36, 663, 1404
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 4 cut(s) 742, 856, 1428, 1461
StyI CCWWGG 1 cut(s) 1012
TaiI ACGT 2 cut(s) 532, 1185
TaqI TCGA 7 cut(s) 146, 186, 199, 433, 589, 602, 1306
TauI GCSGC 2 cut(s) 26, 66
TfiI GAWTC 2 cut(s) 618, 1407
Tru1I TTAA 5 cut(s) 102, 216, 687, 846, 954
Tru9I TTAA 5 cut(s) 102, 216, 687, 846, 954
TseFI GTSAC 2 cut(s) 328, 530
TseI GCWGC 6 cut(s) 60, 507, 853, 1468, 1475, 1480
Tsp45I GTSAC 2 cut(s) 328, 530
TspDTI ATGAA 8 cut(s) 428, 480, 610, 695, 744, 832, 1089, 1221
TspGWI ACGGA 3 cut(s) 371, 912, 997
Tth111I GACNNNGTC 1 cut(s) 497
Van91I CCANNNNNTGG 1 cut(s) 252
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 3 cut(s) 127, 415, 1226
XceI RCATGY 1 cut(s) 1475
XhoI CTCGAG 1 cut(s) 588
XmiI GTMKAC 2 cut(s) 5, 199
XmnI GAANNNNTTC 2 cut(s) 17, 735
XspI CTAG 3 cut(s) 36, 663, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.