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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 95, 239 |
| AcsI | RAATTY | 3 cut(s) | 34, 72, 182 |
| AfaI | GTAC | 2 cut(s) | 313, 426 |
| AgsI | TTSAA | 2 cut(s) | 181, 263 |
| AjiI | CACGTC | 1 cut(s) | 139 |
| AluBI | AGCT | 4 cut(s) | 352, 385, 392, 396 |
| AluI | AGCT | 4 cut(s) | 352, 385, 392, 396 |
| AlwI | GGATC | 2 cut(s) | 95, 239 |
| ApoI | RAATTY | 3 cut(s) | 34, 72, 182 |
| AsuNHI | GCTAGC | 1 cut(s) | 392 |
| BccI | CCATC | 1 cut(s) | 443 |
| BfaI | CTAG | 3 cut(s) | 143, 393, 410 |
| BmcAI | AGTACT | 1 cut(s) | 426 |
| BmgBI | CACGTC | 1 cut(s) | 139 |
| BmtI | GCTAGC | 1 cut(s) | 396 |
| Bse1I | ACTGG | 1 cut(s) | 239 |
| Bse3DI | GCAATG | 2 cut(s) | 166, 286 |
| BseGI | GGATG | 1 cut(s) | 172 |
| BseMI | GCAATG | 2 cut(s) | 166, 286 |
| BseMII | CTCAG | 1 cut(s) | 435 |
| BseNI | ACTGG | 1 cut(s) | 239 |
| Bsp143I | GATC | 3 cut(s) | 100, 108, 244 |
| BspCNI | CTCAG | 1 cut(s) | 434 |
| BspHI | TCATGA | 1 cut(s) | 463 |
| BspOI | GCTAGC | 1 cut(s) | 396 |
| BspPI | GGATC | 2 cut(s) | 95, 239 |
| BsrDI | GCAATG | 2 cut(s) | 166, 286 |
| BsrI | ACTGG | 1 cut(s) | 239 |
| BssMI | GATC | 3 cut(s) | 100, 108, 244 |
| Bst4CI | ACNGT | 1 cut(s) | 10 |
| Bst6I | CTCTTC | 2 cut(s) | 227, 285 |
| BstC8I | GCNNGC | 1 cut(s) | 394 |
| BstDEI | CTNAG | 2 cut(s) | 105, 421 |
| BstF5I | GGATG | 1 cut(s) | 172 |
| BstKTI | GATC | 3 cut(s) | 103, 111, 247 |
| BstMBI | GATC | 3 cut(s) | 100, 108, 244 |
| BstX2I | RGATCY | 1 cut(s) | 100 |
| BstYI | RGATCY | 1 cut(s) | 100 |
| BtrI | CACGTC | 1 cut(s) | 139 |
| BtsCI | GGATG | 1 cut(s) | 172 |
| Cac8I | GCNNGC | 1 cut(s) | 394 |
| CciI | TCATGA | 1 cut(s) | 463 |
| Csp6I | GTAC | 2 cut(s) | 312, 425 |
| CviAII | CATG | 4 cut(s) | 202, 309, 464, 469 |
| CviJI | RGCY | 4 cut(s) | 352, 385, 392, 396 |
| CviKI_1 | RGCY | 4 cut(s) | 352, 385, 392, 396 |
| CviQI | GTAC | 2 cut(s) | 312, 425 |
| DdeI | CTNAG | 2 cut(s) | 105, 421 |
| DpnI | GATC | 3 cut(s) | 102, 110, 246 |
| DpnII | GATC | 3 cut(s) | 100, 108, 244 |
| Eam1104I | CTCTTC | 2 cut(s) | 227, 285 |
| EarI | CTCTTC | 2 cut(s) | 227, 285 |
| EcoRI | GAATTC | 1 cut(s) | 72 |
| FaeI | CATG | 4 cut(s) | 205, 312, 467, 472 |
| FatI | CATG | 4 cut(s) | 201, 308, 463, 468 |
| FokI | GGATG | 1 cut(s) | 179 |
| FspBI | CTAG | 3 cut(s) | 143, 393, 410 |
| Hin1II | CATG | 4 cut(s) | 205, 312, 467, 472 |
| HindIII | AAGCTT | 1 cut(s) | 350 |
| HinfI | GANTC | 1 cut(s) | 235 |
| Hpy188III | TCNNGA | 2 cut(s) | 188, 464 |
| HpyCH4III | ACNGT | 1 cut(s) | 10 |
| HpyCH4IV | ACGT | 2 cut(s) | 138, 224 |
| HpyCH4V | TGCA | 2 cut(s) | 207, 279 |
| HpyF3I | CTNAG | 2 cut(s) | 105, 421 |
| HpySE526I | ACGT | 2 cut(s) | 138, 224 |
| Hsp92II | CATG | 4 cut(s) | 205, 312, 467, 472 |
| Kzo9I | GATC | 3 cut(s) | 100, 108, 244 |
| LpnPI | CCDG | 3 cut(s) | 24, 201, 252 |
| MaeI | CTAG | 3 cut(s) | 143, 393, 410 |
| MaeII | ACGT | 2 cut(s) | 138, 224 |
| MalI | GATC | 3 cut(s) | 102, 110, 246 |
| MboI | GATC | 3 cut(s) | 100, 108, 244 |
| MboII | GAAGA | 3 cut(s) | 203, 244, 302 |
| MflI | RGATCY | 1 cut(s) | 100 |
| MluCI | AATT | 7 cut(s) | 34, 72, 131, 182, 263, 439, 478 |
| MlyI | GAGTC | 1 cut(s) | 244 |
| MmeI | TCCRAC | 1 cut(s) | 63 |
| MnlI | CCTC | 1 cut(s) | 157 |
| MseI | TTAA | 1 cut(s) | 452 |
| NdeII | GATC | 3 cut(s) | 100, 108, 244 |
| NheI | GCTAGC | 1 cut(s) | 392 |
| NlaIII | CATG | 4 cut(s) | 205, 312, 467, 472 |
| PagI | TCATGA | 1 cut(s) | 463 |
| PleI | GAGTC | 1 cut(s) | 243 |
| PpsI | GAGTC | 1 cut(s) | 243 |
| PsuI | RGATCY | 1 cut(s) | 100 |
| RsaI | GTAC | 2 cut(s) | 313, 426 |
| RsaNI | GTAC | 2 cut(s) | 312, 425 |
| SaqAI | TTAA | 1 cut(s) | 452 |
| Sau3AI | GATC | 3 cut(s) | 100, 108, 244 |
| ScaI | AGTACT | 1 cut(s) | 426 |
| SchI | GAGTC | 1 cut(s) | 244 |
| SetI | ASST | 7 cut(s) | 141, 148, 227, 354, 387, 394, 398 |
| Sse9I | AATT | 7 cut(s) | 34, 72, 131, 182, 263, 439, 478 |
| SspMI | CTAG | 3 cut(s) | 143, 393, 410 |
| TaaI | ACNGT | 1 cut(s) | 10 |
| TaiI | ACGT | 2 cut(s) | 141, 227 |
| TasI | AATT | 7 cut(s) | 34, 72, 131, 182, 263, 439, 478 |
| TatI | WGTACW | 2 cut(s) | 311, 424 |
| Tru1I | TTAA | 1 cut(s) | 452 |
| Tru9I | TTAA | 1 cut(s) | 452 |
| TspDTI | ATGAA | 3 cut(s) | 297, 303, 452 |
| XapI | RAATTY | 3 cut(s) | 34, 72, 182 |
| XspI | CTAG | 3 cut(s) | 143, 393, 410 |
| ZrmI | AGTACT | 1 cut(s) | 426 |