MD15G1018100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000270
Physical Location & Seq
Forward (+)
1036785 .. 1038834
2050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1018100.v1.1.491

Sequence Viewer

Length: 981 bp
ATGATCCACATAAATACTACATATGCAATCCAAACGCCCGGCAATGTGTTGCCCTTCCTCCCGCCCCCCCCACACCACAAGATCGCACAAGTGGGATTCATCTGCGATCCCTACTATGCCAGCCAAGAACAAGATAATGGTGGCAGTACTGGTACTAGTATAACCACCAATGATGAGTATCGGTGCAGGGTTGTGCGAATTATTCAACTTGGAAACTATTTATCTCAATTTAAGGTGGACATCTTCTCTTCAGAAACTGGTGAATGGAGAGAATCAGTTGTATCATGTCCACAACAAGGTATTAGTTTTGATTTCACCTTCAACCGTCCAGGCATTGCTTACAATGGAAAGTTGTATTGGCCGAGTTATGGTGGCGTTATCGGGTTTGATCCGTTTGCACCGCACAACGGTAACTATGGGTGTCGGTTCATTGAGTTTGATGAGCCTTTGGACTATACAAAAACGGTTGCGTGTCTAGGGATTTGCCGAGGGCGCCTGCGGATGTGCCAATTACAGACGTGGATGCCACCAGAAACATTTTCTAATCCCTATAATGATGGTCGCCCTCTCATTGTATGTGTCTCGGAGTTGAAAGAAGATGAGGCAGCCGGAGGAGGAAGAGTCAAATGGTGTTTGATACACAAGGCTTCCTTGGACGAATTGGATGTGGGGAAGCATGATTTCCTCAACGATTGTATAAGATTGCGCTACAAGATTCGGGTGCTAGCAGTGGACCCGAATGATGGGGATATTTTATACTTGGAGATGGGTGGACACATAATCAAATGCAACATTCAAGCAAGAACTTTAGAGAAACCTAGAGGTCAAAGGTATGGTCGTAACACTTTTGATTTTTGGCCTTGGAGAGACGTCTGGACATATGTGATTCCATGTTGGCCAACGCCATTGCCTAGAGTTGGACTGCCGCCGCTGTCCAGAAAAAGAAAAAGAGAAGAAAAACAGGCTGCCATAGTCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

327

Amino Acids

37.18

Weight (kDa)

7.93

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 64 - 169 1.6e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000270)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30622 FvH4_4g16921 FvH4_4g17782 FvH4_4g17783 FvH4_4g18120 FvH4_6g01541 FvH4_6g02021 FvH4_6g02041 FvH4_6g02071 FvH4_6g02171 FvH4_6g10900 FvH4_6g37932
malus_domestica MD03G1256400.v1.1 MD05G1244200.v1.1 MD10G1000600.v1.1 MD14G1187900.v1.1 MD15G1018100.v1.1
prunus_persica Prupe.2G073200_v2.0.a1 Prupe.3G246100_v2.0.a1 Prupe.3G265100_v2.0.a1 Prupe.4G034700_v2.0.a1 Prupe.4G052100_v2.0.a1 Prupe.4G053600_v2.0.a1 Prupe.5G068800_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.5G070600_v2.0.a1 Prupe.6G075400_v2.0.a1 Prupe.6G075500_v2.0.a1 Prupe.7G028100_v2.0.a1 Prupe.8G074300_v2.0.a1 Prupe.8G110700_v2.0.a1
pyrus_communis pycom05g22060
rosa_chinensis RchiOBHm_Chr2g0151111 RchiOBHm_Chr3g0449911 RchiOBHm_Chr3g0450211 RchiOBHm_Chr3g0457271
rosa_laevigata RLG00000010311 RLG00000010333 RLG00000020423 RLG00000020530 RLG00000020531 RLG00000025776 RLG00000025778 RLG00000025779 RLG00000025780 RLG00000025782 RLG00000025784 RLG00000025786 RLG00000025789 RLG00000025797 RLG00000025820 RLG00000029780 RLG00000029788 RLG00000029793 RLG00000029794 RLG00000029795 RLG00000029798
rosa_multiflora Rmu_co8159556.1_g000001 Rmu_co8268783.1_g000001 Rmu_co8467147.1_g000001 Rmu_sc0000610.1_g000001 Rmu_sc0000610.1_g000009 Rmu_sc0002725.1_g000015 Rmu_sc0004401.1_g000009 Rmu_sc0004404.1_g000009 Rmu_sc0004404.1_g000016 Rmu_sc0005524.1_g000001 Rmu_sc0005524.1_g000004 Rmu_sc0006867.1_g000019 Rmu_sc0008007.1_g000002 Rmu_sc0010912.1_g000004 Rmu_sc0010912.1_g000005 Rmu_sc0013983.1_g000005 Rmu_sc0020391.1_g000001 Rmu_sc0032173.1_g000001 Rmu_sc0036096.1_g000001 Rmu_sc0043012.1_g000001 Rmu_ssc0000424.1_g000019
rosa_roxburghii Rroxscaffold_2G00097990 Rroxscaffold_6G00420940 Rroxscaffold_6G00427700
rosa_rugosa Rorug02G0424600 Rorug02G0424700 Rorug02G0623900 Rorug05G0571000 Rorug05G0571100 Rorug06G0395100
rosa_samantha Rh1CG104900 Rh1DG114100 Rh2AG485200 Rh2BG497300 Rh2DG508200 Rh2DG508300 Rh3AG022000 Rh3AG022300 Rh3AG022500 Rh3AG022600 Rh3AG022800 Rh3AG023000 Rh3BG020000 Rh3BG022700 Rh3BG022900 Rh3BG023000 Rh3BG023200 Rh3BG023300 Rh3BG023600 Rh3CG018700 Rh3CG021100 Rh3CG021600 Rh3CG021800 Rh3CG021900 Rh3CG022100 Rh3CG022200 Rh3CG022600 Rh3DG022400 Rh3DG022700 Rh3DG022800 Rh3DG023000 Rh3DG023100 Rh3DG023500 Rh6AG511800 Rh6AG512500 Rh6BG523100 Rh6BG523300 Rh6CG528700 Rh6DG514700
rosa_wichuraiana Rw1G008720 Rw2G039750 Rw3G001810 Rw3G002050 Rw6G044530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 873
AccB1I GGYRCC 1 cut(s) 492
AciI CCGC 5 cut(s) 62, 401, 499, 926, 929
AclWI GGATC 2 cut(s) 101, 383
AcoI YGGCCR 2 cut(s) 359, 896
AcuI CTGAAG 1 cut(s) 234
AcyI GRCGYC 2 cut(s) 493, 870
AfaI GTAC 2 cut(s) 148, 154
AfiI CCNNNNNNNGG 5 cut(s) 296, 368, 407, 743, 917
AgsI TTSAA 4 cut(s) 206, 322, 592, 797
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 519
AjnI CCWGG 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 340, 372
Alw26I GTCTC 2 cut(s) 586, 861
AlwI GGATC 2 cut(s) 101, 383
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 3 cut(s) 359, 857, 896
ApeKI GCWGC 2 cut(s) 605, 965
ArsI GACNNNNNNTTYG 2 cut(s) 820, 852
AspLEI GCGC 2 cut(s) 495, 708
AspS9I GGNCC 1 cut(s) 733
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 272, 307
AsuNHI GCTAGC 1 cut(s) 724
AvaII GGWCC 1 cut(s) 733
BalI TGGCCA 1 cut(s) 898
BanI GGYRCC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 617, 952
BccI CCATC 3 cut(s) 551, 737, 760
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 586, 861
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 6 cut(s) 156, 476, 725, 819, 912, 979
BfoI RGCGCY 1 cut(s) 496
BisI GCNGC 4 cut(s) 606, 926, 929, 966
BlsI GCNGC 4 cut(s) 607, 927, 930, 967
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 39, 330
Bme18I GGWCC 1 cut(s) 733
BmgBI CACGTC 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 2 cut(s) 494, 735
BmrFI CCNGG 2 cut(s) 39, 330
BmsI GCATC 1 cut(s) 513
BmtI GCTAGC 1 cut(s) 728
BpuMI CCSGG 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 2 cut(s) 493, 870
BsaJI CCNNGG 3 cut(s) 487, 651, 860
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bsc4I CCNNNNNNNGG 5 cut(s) 296, 368, 407, 743, 917
Bse1I ACTGG 2 cut(s) 154, 262
Bse3DI GCAATG 3 cut(s) 49, 333, 905
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 3 cut(s) 487, 651, 860
BseGI GGATG 3 cut(s) 507, 528, 670
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 5 cut(s) 296, 368, 407, 743, 917
BseMI GCAATG 3 cut(s) 49, 333, 905
BseNI ACTGG 2 cut(s) 154, 262
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 2 cut(s) 617, 952
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 3 cut(s) 361, 859, 898
BshNI GGYRCC 1 cut(s) 492
BsiSI CCGG 2 cut(s) 39, 609
BslI CCNNNNNNNGG 5 cut(s) 296, 368, 407, 743, 917
BsmAI GTCTC 2 cut(s) 586, 861
BsmBI CGTCTC 1 cut(s) 861
BsnI GGCC 3 cut(s) 361, 859, 898
Bsp143I GATC 4 cut(s) 3, 81, 106, 388
BspACI CCGC 5 cut(s) 62, 401, 499, 926, 929
BspANI GGCC 3 cut(s) 361, 859, 898
BspLI GGNNCC 2 cut(s) 494, 735
BspOI GCTAGC 1 cut(s) 728
BspPI GGATC 2 cut(s) 101, 383
BspT107I GGYRCC 1 cut(s) 492
BsrDI GCAATG 3 cut(s) 49, 333, 905
BsrI ACTGG 2 cut(s) 154, 262
BssECI CCNNGG 3 cut(s) 487, 651, 860
BssMI GATC 4 cut(s) 3, 81, 106, 388
BssNI GRCGYC 2 cut(s) 493, 870
BssT1I CCWWGG 2 cut(s) 651, 860
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 3 cut(s) 326, 410, 466
Bst6I CTCTTC 2 cut(s) 253, 613
BstACI GRCGYC 2 cut(s) 493, 870
BstC8I GCNNGC 3 cut(s) 121, 497, 726
BstF5I GGATG 3 cut(s) 507, 528, 670
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 2 cut(s) 495, 708
BstKTI GATC 4 cut(s) 6, 84, 109, 391
BstMAI GTCTC 2 cut(s) 586, 861
BstMBI GATC 4 cut(s) 3, 81, 106, 388
BstMWI GCNNNNNNNGC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 37, 328
BstV1I GCAGC 2 cut(s) 617, 952
BsuRI GGCC 3 cut(s) 361, 859, 898
BtrI CACGTC 1 cut(s) 519
BtsCI GGATG 3 cut(s) 507, 528, 670
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 121, 497, 726
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 2 cut(s) 495, 708
Cfr13I GGNCC 1 cut(s) 733
Csp6I GTAC 2 cut(s) 147, 153
CviAII CATG 3 cut(s) 285, 677, 891
CviJI RGCY 8 cut(s) 123, 361, 445, 608, 647, 859, 898, 965
CviKI_1 RGCY 8 cut(s) 123, 361, 445, 608, 647, 859, 898, 965
CviQI GTAC 2 cut(s) 147, 153
DinI GGCGCC 1 cut(s) 494
DpnI GATC 4 cut(s) 5, 83, 108, 390
DpnII GATC 4 cut(s) 3, 81, 106, 388
EaeI YGGCCR 2 cut(s) 359, 896
Eam1104I CTCTTC 2 cut(s) 253, 613
EarI CTCTTC 2 cut(s) 253, 613
Eco130I CCWWGG 2 cut(s) 651, 860
Eco47I GGWCC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 651, 860
EgeI GGCGCC 1 cut(s) 494
EheI GGCGCC 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 651, 860
Esp3I CGTCTC 1 cut(s) 861
FaeI CATG 3 cut(s) 288, 680, 894
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FatI CATG 3 cut(s) 284, 676, 890
FauI CCCGC 1 cut(s) 69
FauNDI CATATG 2 cut(s) 22, 880
Fnu4HI GCNGC 4 cut(s) 606, 926, 929, 966
FokI GGATG 3 cut(s) 514, 535, 677
Fsp4HI GCNGC 4 cut(s) 606, 926, 929, 966
FspBI CTAG 6 cut(s) 156, 476, 725, 819, 912, 979
GlaI GCGC 2 cut(s) 494, 707
GluI GCNGC 4 cut(s) 606, 926, 929, 966
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 3 cut(s) 361, 859, 898
HapII CCGG 2 cut(s) 39, 609
HhaI GCGC 2 cut(s) 495, 708
Hin1I GRCGYC 2 cut(s) 493, 870
Hin1II CATG 3 cut(s) 288, 680, 894
Hin6I GCGC 2 cut(s) 493, 706
HinP1I GCGC 2 cut(s) 493, 706
HinfI GANTC 5 cut(s) 96, 272, 621, 715, 886
HpaII CCGG 2 cut(s) 39, 609
HphI GGTGA 2 cut(s) 272, 307
Hpy166II GTNNAC 4 cut(s) 238, 290, 733, 773
Hpy188I TCNGA 2 cut(s) 253, 586
Hpy188III TCNNGA 2 cut(s) 874, 936
Hpy8I GTNNAC 4 cut(s) 238, 290, 733, 773
HpyAV CCTTC 2 cut(s) 64, 328
HpyCH4III ACNGT 3 cut(s) 326, 410, 466
HpyCH4IV ACGT 2 cut(s) 518, 870
HpyCH4V TGCA 4 cut(s) 26, 186, 398, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 492
HpySE526I ACGT 2 cut(s) 518, 870
Hsp92I GRCGYC 2 cut(s) 493, 870
Hsp92II CATG 3 cut(s) 288, 680, 894
HspAI GCGC 2 cut(s) 493, 706
KasI GGCGCC 1 cut(s) 492
Kzo9I GATC 4 cut(s) 3, 81, 106, 388
Lsp1109I GCAGC 2 cut(s) 617, 952
LweI GCATC 1 cut(s) 513
MaeI CTAG 6 cut(s) 156, 476, 725, 819, 912, 979
MaeII ACGT 2 cut(s) 518, 870
MaeIII GTNAC 2 cut(s) 410, 839
MalI GATC 4 cut(s) 5, 83, 108, 390
MboI GATC 4 cut(s) 3, 81, 106, 388
MboII GAAGA 5 cut(s) 235, 240, 608, 630, 965
MlsI TGGCCA 1 cut(s) 898
MluCI AATT 4 cut(s) 198, 227, 509, 659
MluNI TGGCCA 1 cut(s) 898
Mly113I GGCGCC 1 cut(s) 493
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 8 cut(s) 68, 482, 576, 595, 605, 608, 695, 815
Mox20I TGGCCA 1 cut(s) 898
MscI TGGCCA 1 cut(s) 898
MseI TTAA 1 cut(s) 231
Msp20I TGGCCA 1 cut(s) 898
MspA1I CMGCKG 1 cut(s) 931
MspI CCGG 2 cut(s) 39, 609
MspR9I CCNGG 2 cut(s) 39, 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 492
NarI GGCGCC 1 cut(s) 493
NciI CCSGG 1 cut(s) 39
NdeI CATATG 2 cut(s) 22, 880
NdeII GATC 4 cut(s) 3, 81, 106, 388
NheI GCTAGC 1 cut(s) 724
NlaIII CATG 3 cut(s) 288, 680, 894
NlaIV GGNNCC 2 cut(s) 494, 735
NmeAIII GCCGAG 2 cut(s) 387, 512
PfeI GAWTC 4 cut(s) 96, 272, 715, 886
PkrI GCNGC 4 cut(s) 607, 927, 930, 967
PleI GAGTC 1 cut(s) 629
PluTI GGCGCC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 494, 735
PspPI GGNCC 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 148, 154
RsaNI GTAC 2 cut(s) 147, 153
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 606, 926, 929, 966
Sau3AI GATC 4 cut(s) 3, 81, 106, 388
Sau96I GGNCC 1 cut(s) 733
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 2 cut(s) 39, 330
SetI ASST 8 cut(s) 237, 301, 320, 521, 820, 826, 833, 873
SfaNI GCATC 1 cut(s) 513
SfoI GGCGCC 1 cut(s) 494
SinI GGWCC 1 cut(s) 733
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 4 cut(s) 198, 227, 509, 659
SsiI CCGC 5 cut(s) 62, 401, 499, 926, 929
SspDI GGCGCC 1 cut(s) 492
SspMI CTAG 6 cut(s) 156, 476, 725, 819, 912, 979
StyD4I CCNGG 2 cut(s) 37, 328
StyI CCWWGG 2 cut(s) 651, 860
TaaI ACNGT 3 cut(s) 326, 410, 466
TaiI ACGT 2 cut(s) 521, 873
TasI AATT 4 cut(s) 198, 227, 509, 659
TatI WGTACW 1 cut(s) 146
TauI GCSGC 2 cut(s) 928, 931
TfiI GAWTC 4 cut(s) 96, 272, 715, 886
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 735
TseI GCWGC 2 cut(s) 605, 965
TspDTI ATGAA 2 cut(s) 88, 418
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 735
VpaK11BI GGWCC 1 cut(s) 733
XspI CTAG 6 cut(s) 156, 476, 725, 819, 912, 979
ZraI GACGTC 1 cut(s) 871
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.