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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 1032, 1469 |
| AccII | CGCG | 1 cut(s) | 438 |
| AciI | CCGC | 4 cut(s) | 98, 537, 1202, 1378 |
| AclI | AACGTT | 1 cut(s) | 268 |
| AclWI | GGATC | 5 cut(s) | 938, 951, 1073, 1106, 1331 |
| AcoI | YGGCCR | 2 cut(s) | 1375, 1496 |
| AcsI | RAATTY | 2 cut(s) | 236, 776 |
| AcuI | CTGAAG | 1 cut(s) | 352 |
| AcvI | CACGTG | 1 cut(s) | 1392 |
| AcyI | GRCGYC | 1 cut(s) | 1470 |
| AfaI | GTAC | 2 cut(s) | 358, 1485 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 458, 615, 845 |
| AflIII | ACRYGT | 2 cut(s) | 256, 1391 |
| AgsI | TTSAA | 4 cut(s) | 124, 209, 775, 781 |
| AjnI | CCWGG | 1 cut(s) | 717 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 889, 921 |
| AluBI | AGCT | 6 cut(s) | 162, 412, 418, 464, 1177, 1331 |
| AluI | AGCT | 6 cut(s) | 162, 412, 418, 464, 1177, 1331 |
| Alw21I | GWGCWC | 1 cut(s) | 414 |
| Alw26I | GTCTC | 3 cut(s) | 114, 797, 1454 |
| AlwI | GGATC | 5 cut(s) | 938, 951, 1073, 1106, 1331 |
| Ama87I | CYCGRG | 1 cut(s) | 413 |
| AoxI | GGCC | 3 cut(s) | 494, 1375, 1496 |
| ApeKI | GCWGC | 1 cut(s) | 77 |
| ApoI | RAATTY | 2 cut(s) | 236, 776 |
| AseI | ATTAAT | 3 cut(s) | 987, 1274, 1430 |
| AspLEI | GCGC | 1 cut(s) | 1472 |
| AspS9I | GGNCC | 1 cut(s) | 494 |
| AsuC2I | CCSGG | 1 cut(s) | 1296 |
| AsuHPI | GGTGA | 1 cut(s) | 1202 |
| AsuII | TTCGAA | 1 cut(s) | 1263 |
| AsuNHI | GCTAGC | 1 cut(s) | 1327 |
| AvaI | CYCGRG | 1 cut(s) | 413 |
| BalI | TGGCCA | 1 cut(s) | 1498 |
| BamHI | GGATCC | 1 cut(s) | 943 |
| BanI | GGYRCC | 2 cut(s) | 1032, 1469 |
| BanII | GRGCYC | 2 cut(s) | 414, 839 |
| BbrPI | CACGTG | 1 cut(s) | 1392 |
| BbsI | GAAGAC | 2 cut(s) | 593, 876 |
| Bbv12I | GWGCWC | 1 cut(s) | 414 |
| BbvI | GCAGC | 1 cut(s) | 64 |
| BccI | CCATC | 2 cut(s) | 485, 1181 |
| BceAI | ACGGC | 3 cut(s) | 291, 862, 1061 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 578, 612 |
| BciT130I | CCWGG | 1 cut(s) | 719 |
| BclI | TGATCA | 2 cut(s) | 679, 1069 |
| BcnI | CCSGG | 1 cut(s) | 1296 |
| BcoDI | GTCTC | 3 cut(s) | 114, 797, 1454 |
| BfaI | CTAG | 2 cut(s) | 1328, 1364 |
| BfmI | CTRYAG | 2 cut(s) | 465, 622 |
| BfoI | RGCGCY | 1 cut(s) | 1473 |
| BglII | AGATCT | 1 cut(s) | 789 |
| BisI | GCNGC | 2 cut(s) | 78, 1378 |
| BlsI | GCNGC | 2 cut(s) | 79, 1379 |
| BmcAI | AGTACT | 1 cut(s) | 358 |
| Bme1390I | CCNGG | 2 cut(s) | 719, 1296 |
| BmeT110I | CYCGRG | 1 cut(s) | 413 |
| BmgT120I | GGNCC | 1 cut(s) | 494 |
| BmiI | GGNNCC | 4 cut(s) | 320, 945, 1034, 1471 |
| BmrFI | CCNGG | 2 cut(s) | 719, 1296 |
| BmrI | ACTGGG | 1 cut(s) | 1152 |
| BmsI | GCATC | 3 cut(s) | 33, 364, 1189 |
| BmtI | GCTAGC | 1 cut(s) | 1331 |
| BmuI | ACTGGG | 1 cut(s) | 1152 |
| BpiI | GAAGAC | 2 cut(s) | 593, 876 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 779, 811 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1247 |
| Bpu14I | TTCGAA | 1 cut(s) | 1263 |
| BpuMI | CCSGG | 1 cut(s) | 1296 |
| BsaAI | YACGTR | 2 cut(s) | 1028, 1392 |
| BsaHI | GRCGYC | 1 cut(s) | 1470 |
| BsaJI | CCNNGG | 3 cut(s) | 614, 1036, 1490 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 458, 615, 845 |
| Bse1I | ACTGG | 2 cut(s) | 702, 1158 |
| Bse3DI | GCAATG | 2 cut(s) | 523, 882 |
| BseBI | CCWGG | 1 cut(s) | 719 |
| BseDI | CCNNGG | 3 cut(s) | 614, 1036, 1490 |
| BseGI | GGATG | 2 cut(s) | 379, 714 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 458, 615, 845 |
| BseMI | GCAATG | 2 cut(s) | 523, 882 |
| BseMII | CTCAG | 2 cut(s) | 125, 792 |
| BseNI | ACTGG | 2 cut(s) | 702, 1158 |
| BseRI | GAGGAG | 1 cut(s) | 1053 |
| BseXI | GCAGC | 1 cut(s) | 64 |
| Bsh1236I | CGCG | 1 cut(s) | 438 |
| BshFI | GGCC | 3 cut(s) | 496, 1377, 1498 |
| BshNI | GGYRCC | 2 cut(s) | 1032, 1469 |
| BsiHKAI | GWGCWC | 1 cut(s) | 414 |
| BsiHKCI | CYCGRG | 1 cut(s) | 413 |
| BsiSI | CCGG | 4 cut(s) | 50, 143, 407, 1296 |
| BslFI | GGGAC | 3 cut(s) | 100, 1088, 1278 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 458, 615, 845 |
| BsmAI | GTCTC | 3 cut(s) | 114, 797, 1454 |
| BsmBI | CGTCTC | 1 cut(s) | 797 |
| BsmFI | GGGAC | 3 cut(s) | 100, 1088, 1278 |
| BsmI | GAATGC | 1 cut(s) | 971 |
| BsnI | GGCC | 3 cut(s) | 496, 1377, 1498 |
| BsoBI | CYCGRG | 1 cut(s) | 413 |
| Bsp119I | TTCGAA | 1 cut(s) | 1263 |
| Bsp1286I | GDGCHC | 2 cut(s) | 414, 839 |
| BspACI | CCGC | 4 cut(s) | 98, 537, 1202, 1378 |
| BspANI | GGCC | 3 cut(s) | 496, 1377, 1498 |
| BspCNI | CTCAG | 2 cut(s) | 124, 793 |
| BspFNI | CGCG | 1 cut(s) | 438 |
| BspHI | TCATGA | 2 cut(s) | 57, 181 |
| BspLI | GGNNCC | 4 cut(s) | 320, 945, 1034, 1471 |
| BspOI | GCTAGC | 1 cut(s) | 1331 |
| BspPI | GGATC | 5 cut(s) | 938, 951, 1073, 1106, 1331 |
| BspT104I | TTCGAA | 1 cut(s) | 1263 |
| BspT107I | GGYRCC | 2 cut(s) | 1032, 1469 |
| BsrDI | GCAATG | 2 cut(s) | 523, 882 |
| BsrI | ACTGG | 2 cut(s) | 702, 1158 |
| BssECI | CCNNGG | 3 cut(s) | 614, 1036, 1490 |
| BssNI | GRCGYC | 1 cut(s) | 1470 |
| BssT1I | CCWWGG | 1 cut(s) | 1490 |
| Bst2UI | CCWGG | 1 cut(s) | 719 |
| Bst4CI | ACNGT | 1 cut(s) | 1324 |
| Bst6I | CTCTTC | 1 cut(s) | 802 |
| BstACI | GRCGYC | 1 cut(s) | 1470 |
| BstBAI | YACGTR | 2 cut(s) | 1028, 1392 |
| BstBI | TTCGAA | 1 cut(s) | 1263 |
| BstC8I | GCNNGC | 2 cut(s) | 514, 1329 |
| BstDEI | CTNAG | 6 cut(s) | 105, 111, 523, 801, 1049, 1247 |
| BstF5I | GGATG | 2 cut(s) | 379, 714 |
| BstFNI | CGCG | 1 cut(s) | 438 |
| BstH2I | RGCGCY | 1 cut(s) | 1473 |
| BstHHI | GCGC | 1 cut(s) | 1472 |
| BstMAI | GTCTC | 3 cut(s) | 114, 797, 1454 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 83, 1221 |
| BstNI | CCWGG | 1 cut(s) | 719 |
| BstNSI | RCATGY | 1 cut(s) | 516 |
| BstSCI | CCNGG | 2 cut(s) | 717, 1294 |
| BstSFI | CTRYAG | 2 cut(s) | 465, 622 |
| BstUI | CGCG | 1 cut(s) | 438 |
| BstV1I | GCAGC | 1 cut(s) | 64 |
| BstV2I | GAAGAC | 2 cut(s) | 593, 876 |
| BstX2I | RGATCY | 2 cut(s) | 789, 943 |
| BstYI | RGATCY | 2 cut(s) | 789, 943 |
| BsuRI | GGCC | 3 cut(s) | 496, 1377, 1498 |
| BtsCI | GGATG | 2 cut(s) | 379, 714 |
| BtsI | GCAGTG | 1 cut(s) | 301 |
| BtsIMutI | CAGTG | 3 cut(s) | 254, 301, 1329 |
| Cac8I | GCNNGC | 2 cut(s) | 514, 1329 |
| CciI | TCATGA | 2 cut(s) | 57, 181 |
| CfoI | GCGC | 1 cut(s) | 1472 |
| Cfr13I | GGNCC | 1 cut(s) | 494 |
| Csp6I | GTAC | 2 cut(s) | 357, 1484 |
| CviAII | CATG | 5 cut(s) | 58, 153, 182, 513, 1123 |
| CviQI | GTAC | 2 cut(s) | 357, 1484 |
| DdeI | CTNAG | 6 cut(s) | 105, 111, 523, 801, 1049, 1247 |
| DinI | GGCGCC | 1 cut(s) | 1471 |
| EaeI | YGGCCR | 2 cut(s) | 1375, 1496 |
| Eam1104I | CTCTTC | 1 cut(s) | 802 |
| EarI | CTCTTC | 1 cut(s) | 802 |
| EciI | GGCGGA | 1 cut(s) | 87 |
| Ecl136II | GAGCTC | 1 cut(s) | 412 |
| Eco130I | CCWWGG | 1 cut(s) | 1490 |
| Eco24I | GRGCYC | 2 cut(s) | 414, 839 |
| Eco32I | GATATC | 3 cut(s) | 63, 1093, 1301 |
| Eco53kI | GAGCTC | 1 cut(s) | 412 |
| Eco57I | CTGAAG | 1 cut(s) | 352 |
| Eco72I | CACGTG | 1 cut(s) | 1392 |
| Eco88I | CYCGRG | 1 cut(s) | 413 |
| EcoICRI | GAGCTC | 1 cut(s) | 412 |
| EcoRII | CCWGG | 1 cut(s) | 717 |
| EcoRV | GATATC | 3 cut(s) | 63, 1093, 1301 |
| EcoT14I | CCWWGG | 1 cut(s) | 1490 |
| EcoT38I | GRGCYC | 2 cut(s) | 414, 839 |
| EgeI | GGCGCC | 1 cut(s) | 1471 |
| EheI | GGCGCC | 1 cut(s) | 1471 |
| ErhI | CCWWGG | 1 cut(s) | 1490 |
| Esp3I | CGTCTC | 1 cut(s) | 797 |
| FaeI | CATG | 5 cut(s) | 61, 156, 185, 516, 1126 |
| FaqI | GGGAC | 3 cut(s) | 100, 1088, 1278 |
| FatI | CATG | 5 cut(s) | 57, 152, 181, 512, 1122 |
| FauNDI | CATATG | 1 cut(s) | 1441 |
| FbaI | TGATCA | 2 cut(s) | 679, 1069 |
| Fnu4HI | GCNGC | 2 cut(s) | 78, 1378 |
| FokI | GGATG | 2 cut(s) | 386, 701 |
| FriOI | GRGCYC | 2 cut(s) | 414, 839 |
| Fsp4HI | GCNGC | 2 cut(s) | 78, 1378 |
| FspBI | CTAG | 2 cut(s) | 1328, 1364 |
| GlaI | GCGC | 1 cut(s) | 1471 |
| GluI | GCNGC | 2 cut(s) | 78, 1378 |
| HaeII | RGCGCY | 1 cut(s) | 1473 |
| HaeIII | GGCC | 3 cut(s) | 496, 1377, 1498 |
| HapII | CCGG | 4 cut(s) | 50, 143, 407, 1296 |
| HhaI | GCGC | 1 cut(s) | 1472 |
| Hin1I | GRCGYC | 1 cut(s) | 1470 |
| Hin1II | CATG | 5 cut(s) | 61, 156, 185, 516, 1126 |
| Hin6I | GCGC | 1 cut(s) | 1470 |
| HinP1I | GCGC | 1 cut(s) | 1470 |
| HindIII | AAGCTT | 1 cut(s) | 160 |
| HinfI | GANTC | 6 cut(s) | 194, 642, 821, 897, 1053, 1312 |
| HpaII | CCGG | 4 cut(s) | 50, 143, 407, 1296 |
| HphI | GGTGA | 1 cut(s) | 1202 |
| Hpy188I | TCNGA | 8 cut(s) | 289, 348, 423, 431, 461, 684, 802, 1135 |
| Hpy188III | TCNNGA | 5 cut(s) | 58, 182, 962, 1364, 1403 |
| Hpy99I | CGWCG | 1 cut(s) | 352 |
| HpyAV | CCTTC | 7 cut(s) | 686, 1030, 1093, 1140, 1163, 1483, 1517 |
| HpyCH4III | ACNGT | 1 cut(s) | 1324 |
| HpyCH4IV | ACGT | 5 cut(s) | 256, 268, 1027, 1291, 1391 |
| HpyCH4V | TGCA | 7 cut(s) | 264, 377, 516, 573, 1059, 1126, 1224 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 83, 1221 |
| HpyF3I | CTNAG | 6 cut(s) | 105, 111, 523, 801, 1049, 1247 |
| HpySE526I | ACGT | 5 cut(s) | 256, 268, 1027, 1291, 1391 |
| Hsp92I | GRCGYC | 1 cut(s) | 1470 |
| Hsp92II | CATG | 5 cut(s) | 61, 156, 185, 516, 1126 |
| HspAI | GCGC | 1 cut(s) | 1470 |
| KasI | GGCGCC | 1 cut(s) | 1469 |
| Ksp22I | TGATCA | 2 cut(s) | 679, 1069 |
| LmnI | GCTCC | 5 cut(s) | 324, 409, 461, 770, 1174 |
| Lsp1109I | GCAGC | 1 cut(s) | 64 |
| LweI | GCATC | 3 cut(s) | 33, 364, 1189 |
| MaeI | CTAG | 2 cut(s) | 1328, 1364 |
| MaeII | ACGT | 5 cut(s) | 256, 268, 1027, 1291, 1391 |
| MaeIII | GTNAC | 2 cut(s) | 1387, 1394 |
| MboII | GAAGA | 7 cut(s) | 358, 593, 680, 784, 789, 881, 1023 |
| MflI | RGATCY | 2 cut(s) | 789, 943 |
| MhlI | GDGCHC | 2 cut(s) | 414, 839 |
| MlsI | TGGCCA | 1 cut(s) | 1498 |
| MluNI | TGGCCA | 1 cut(s) | 1498 |
| Mly113I | GGCGCC | 1 cut(s) | 1470 |
| MlyI | GAGTC | 2 cut(s) | 188, 1306 |
| MmeI | TCCRAC | 2 cut(s) | 371, 921 |
| Mox20I | TGGCCA | 1 cut(s) | 1498 |
| MscI | TGGCCA | 1 cut(s) | 1498 |
| MseI | TTAA | 5 cut(s) | 131, 987, 1274, 1415, 1430 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1121 |
| Msp20I | TGGCCA | 1 cut(s) | 1498 |
| MspI | CCGG | 4 cut(s) | 50, 143, 407, 1296 |
| MspR9I | CCNGG | 2 cut(s) | 719, 1296 |
| Mva1269I | GAATGC | 1 cut(s) | 971 |
| MvaI | CCWGG | 1 cut(s) | 719 |
| MvnI | CGCG | 1 cut(s) | 438 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 83, 1221 |
| NarI | GGCGCC | 1 cut(s) | 1470 |
| NciI | CCSGG | 1 cut(s) | 1296 |
| NdeI | CATATG | 1 cut(s) | 1441 |
| NheI | GCTAGC | 1 cut(s) | 1327 |
| NlaIII | CATG | 5 cut(s) | 61, 156, 185, 516, 1126 |
| NlaIV | GGNNCC | 4 cut(s) | 320, 945, 1034, 1471 |
| NmeAIII | GCCGAG | 1 cut(s) | 1061 |
| NmuCI | GTSAC | 1 cut(s) | 1387 |
| NspI | RCATGY | 1 cut(s) | 516 |
| NspV | TTCGAA | 1 cut(s) | 1263 |
| PaeI | GCATGC | 1 cut(s) | 516 |
| PaeR7I | CTCGAG | 1 cut(s) | 413 |
| PagI | TCATGA | 2 cut(s) | 57, 181 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1033 |
| PctI | GAATGC | 1 cut(s) | 971 |
| PfeI | GAWTC | 4 cut(s) | 642, 821, 897, 1053 |
| PfoI | TCCNGGA | 1 cut(s) | 1294 |
| PkrI | GCNGC | 2 cut(s) | 79, 1379 |
| PleI | GAGTC | 2 cut(s) | 188, 1306 |
| PluTI | GGCGCC | 1 cut(s) | 1473 |
| PmaCI | CACGTG | 1 cut(s) | 1392 |
| PmlI | CACGTG | 1 cut(s) | 1392 |
| PpsI | GAGTC | 2 cut(s) | 188, 1306 |
| Ppu21I | YACGTR | 2 cut(s) | 1028, 1392 |
| PshBI | ATTAAT | 3 cut(s) | 987, 1274, 1430 |
| Psp124BI | GAGCTC | 1 cut(s) | 414 |
| Psp1406I | AACGTT | 1 cut(s) | 268 |
| Psp6I | CCWGG | 1 cut(s) | 717 |
| PspCI | CACGTG | 1 cut(s) | 1392 |
| PspGI | CCWGG | 1 cut(s) | 717 |
| PspN4I | GGNNCC | 4 cut(s) | 320, 945, 1034, 1471 |
| PspPI | GGNCC | 1 cut(s) | 494 |
| PspXI | VCTCGAGB | 1 cut(s) | 413 |
| PsuI | RGATCY | 2 cut(s) | 789, 943 |
| RsaI | GTAC | 2 cut(s) | 358, 1485 |
| RsaNI | GTAC | 2 cut(s) | 357, 1484 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1121 |
| SacI | GAGCTC | 1 cut(s) | 414 |
| SaqAI | TTAA | 5 cut(s) | 131, 987, 1274, 1415, 1430 |
| SatI | GCNGC | 2 cut(s) | 78, 1378 |
| Sau96I | GGNCC | 1 cut(s) | 494 |
| ScaI | AGTACT | 1 cut(s) | 358 |
| SchI | GAGTC | 2 cut(s) | 188, 1306 |
| ScrFI | CCNGG | 2 cut(s) | 719, 1296 |
| SduI | GDGCHC | 2 cut(s) | 414, 839 |
| SfaNI | GCATC | 3 cut(s) | 33, 364, 1189 |
| SfcI | CTRYAG | 2 cut(s) | 465, 622 |
| SfoI | GGCGCC | 1 cut(s) | 1471 |
| Sfr274I | CTCGAG | 1 cut(s) | 413 |
| SfuI | TTCGAA | 1 cut(s) | 1263 |
| SlaI | CTCGAG | 1 cut(s) | 413 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1121 |
| SmlI | CTYRAG | 1 cut(s) | 413 |
| SmoI | CTYRAG | 1 cut(s) | 413 |
| SphI | GCATGC | 1 cut(s) | 516 |
| SsiI | CCGC | 4 cut(s) | 98, 537, 1202, 1378 |
| SspDI | GGCGCC | 1 cut(s) | 1469 |
| SspI | AATATT | 1 cut(s) | 395 |
| SspMI | CTAG | 2 cut(s) | 1328, 1364 |
| SstI | GAGCTC | 1 cut(s) | 414 |
| StyD4I | CCNGG | 2 cut(s) | 717, 1294 |
| StyI | CCWWGG | 1 cut(s) | 1490 |
| TaaI | ACNGT | 1 cut(s) | 1324 |
| TaiI | ACGT | 5 cut(s) | 259, 271, 1030, 1294, 1394 |
| TaqI | TCGA | 3 cut(s) | 414, 1263, 1335 |
| TatI | WGTACW | 2 cut(s) | 356, 1483 |
| TauI | GCSGC | 1 cut(s) | 1380 |
| TfiI | GAWTC | 4 cut(s) | 642, 821, 897, 1053 |
| Tru1I | TTAA | 5 cut(s) | 131, 987, 1274, 1415, 1430 |
| Tru9I | TTAA | 5 cut(s) | 131, 987, 1274, 1415, 1430 |
| TscAI | CASTG | 3 cut(s) | 254, 308, 1329 |
| TseFI | GTSAC | 1 cut(s) | 1387 |
| TseI | GCWGC | 1 cut(s) | 77 |
| Tsp45I | GTSAC | 1 cut(s) | 1387 |
| TspDTI | ATGAA | 5 cut(s) | 170, 306, 567, 634, 1458 |
| TspGWI | ACGGA | 1 cut(s) | 365 |
| TspRI | CASTG | 3 cut(s) | 254, 308, 1329 |
| VspI | ATTAAT | 3 cut(s) | 987, 1274, 1430 |
| XapI | RAATTY | 2 cut(s) | 236, 776 |
| XbaI | TCTAGA | 1 cut(s) | 1363 |
| XceI | RCATGY | 1 cut(s) | 516 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 932 |
| XhoI | CTCGAG | 1 cut(s) | 413 |
| XspI | CTAG | 2 cut(s) | 1328, 1364 |
| ZrmI | AGTACT | 1 cut(s) | 358 |