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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 445 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 512 |
| Acc36I | ACCTGC | 1 cut(s) | 445 |
| AccI | GTMKAC | 2 cut(s) | 546, 983 |
| AccII | CGCG | 3 cut(s) | 122, 249, 513 |
| AciI | CCGC | 4 cut(s) | 120, 122, 153, 387 |
| AclWI | GGATC | 2 cut(s) | 903, 982 |
| AcsI | RAATTY | 3 cut(s) | 1296, 1370, 1397 |
| AcuI | CTGAAG | 2 cut(s) | 401, 1326 |
| AdeI | CACNNNGTG | 1 cut(s) | 213 |
| AfaI | GTAC | 5 cut(s) | 190, 689, 747, 806, 1191 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 64, 713, 1391 |
| AgsI | TTSAA | 5 cut(s) | 132, 374, 1023, 1226, 1330 |
| AjnI | CCWGG | 3 cut(s) | 970, 1215, 1384 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 149, 181 |
| AluBI | AGCT | 5 cut(s) | 235, 379, 766, 1129, 1144 |
| AluI | AGCT | 5 cut(s) | 235, 379, 766, 1129, 1144 |
| Alw26I | GTCTC | 4 cut(s) | 5, 582, 849, 1107 |
| AlwI | GGATC | 2 cut(s) | 903, 982 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 557, 977 |
| Ama87I | CYCGRG | 2 cut(s) | 64, 708 |
| AoxI | GGCC | 4 cut(s) | 53, 67, 82, 925 |
| ApaI | GGGCCC | 1 cut(s) | 57 |
| ApeKI | GCWGC | 2 cut(s) | 160, 232 |
| ApoI | RAATTY | 3 cut(s) | 1296, 1370, 1397 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 369, 1371 |
| AspLEI | GCGC | 1 cut(s) | 513 |
| AspS9I | GGNCC | 3 cut(s) | 53, 54, 67 |
| AsuC2I | CCSGG | 3 cut(s) | 52, 709, 710 |
| AsuHPI | GGTGA | 4 cut(s) | 469, 1049, 1406, 1415 |
| AsuII | TTCGAA | 1 cut(s) | 1030 |
| AvaI | CYCGRG | 2 cut(s) | 64, 708 |
| AxyI | CCTNAGG | 2 cut(s) | 294, 1290 |
| BaeGI | GKGCMC | 1 cut(s) | 57 |
| BanII | GRGCYC | 1 cut(s) | 57 |
| BbsI | GAAGAC | 1 cut(s) | 23 |
| BbvI | GCAGC | 2 cut(s) | 172, 244 |
| BccI | CCATC | 4 cut(s) | 31, 1103, 1112, 1330 |
| BceAI | ACGGC | 2 cut(s) | 97, 207 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 536, 570 |
| BciT130I | CCWGG | 3 cut(s) | 972, 1217, 1386 |
| BclI | TGATCA | 3 cut(s) | 222, 328, 699 |
| BcnI | CCSGG | 3 cut(s) | 52, 709, 710 |
| BcoDI | GTCTC | 4 cut(s) | 5, 582, 849, 1107 |
| BfaI | CTAG | 2 cut(s) | 137, 1181 |
| BfmI | CTRYAG | 1 cut(s) | 1197 |
| BfuAI | ACCTGC | 1 cut(s) | 445 |
| BglII | AGATCT | 2 cut(s) | 537, 1092 |
| BisI | GCNGC | 4 cut(s) | 120, 153, 161, 233 |
| BlsI | GCNGC | 4 cut(s) | 121, 154, 162, 234 |
| Bme1390I | CCNGG | 6 cut(s) | 52, 709, 710, 972, 1217, 1386 |
| BmeT110I | CYCGRG | 2 cut(s) | 64, 708 |
| BmgT120I | GGNCC | 3 cut(s) | 53, 54, 67 |
| BmiI | GGNNCC | 3 cut(s) | 55, 56, 1248 |
| BmrFI | CCNGG | 6 cut(s) | 52, 709, 710, 972, 1217, 1386 |
| BmrI | ACTGGG | 1 cut(s) | 996 |
| BmsI | GCATC | 2 cut(s) | 328, 544 |
| BmuI | ACTGGG | 1 cut(s) | 996 |
| BpiI | GAAGAC | 1 cut(s) | 23 |
| BpmI | CTGGAG | 1 cut(s) | 204 |
| Bpu14I | TTCGAA | 1 cut(s) | 1030 |
| BpuEI | CTTGAG | 1 cut(s) | 647 |
| BpuMI | CCSGG | 3 cut(s) | 52, 709, 710 |
| BsaAI | YACGTR | 1 cut(s) | 1061 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1321 |
| BsaI | GGTCTC | 1 cut(s) | 849 |
| BsaJI | CCNNGG | 8 cut(s) | 120, 280, 707, 708, 709, 928, 1149, 1385 |
| BsaWI | WCCGGW | 1 cut(s) | 33 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 32, 62 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 64, 713, 1391 |
| Bse1I | ACTGG | 1 cut(s) | 991 |
| Bse21I | CCTNAGG | 2 cut(s) | 294, 1290 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1321 |
| BseBI | CCWGG | 3 cut(s) | 972, 1217, 1386 |
| BseDI | CCNNGG | 8 cut(s) | 120, 280, 707, 708, 709, 928, 1149, 1385 |
| BseGI | GGATG | 5 cut(s) | 42, 225, 331, 1322, 1429 |
| BseJI | GATNNNNATC | 1 cut(s) | 1321 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 64, 713, 1391 |
| BseMII | CTCAG | 2 cut(s) | 285, 1281 |
| BseNI | ACTGG | 1 cut(s) | 991 |
| BseRI | GAGGAG | 2 cut(s) | 23, 311 |
| BseSI | GKGCMC | 1 cut(s) | 57 |
| BseXI | GCAGC | 2 cut(s) | 172, 244 |
| Bsh1236I | CGCG | 3 cut(s) | 122, 249, 513 |
| BshFI | GGCC | 4 cut(s) | 55, 69, 84, 927 |
| BsiHKCI | CYCGRG | 2 cut(s) | 64, 708 |
| BsiSI | CCGG | 4 cut(s) | 34, 51, 198, 709 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 64, 713, 1391 |
| BsmAI | GTCTC | 4 cut(s) | 5, 582, 849, 1107 |
| BsmBI | CGTCTC | 2 cut(s) | 5, 1107 |
| BsmI | GAATGC | 1 cut(s) | 466 |
| BsnI | GGCC | 4 cut(s) | 55, 69, 84, 927 |
| Bso31I | GGTCTC | 1 cut(s) | 849 |
| BsoBI | CYCGRG | 2 cut(s) | 64, 708 |
| Bsp119I | TTCGAA | 1 cut(s) | 1030 |
| Bsp120I | GGGCCC | 1 cut(s) | 53 |
| Bsp1286I | GDGCHC | 1 cut(s) | 57 |
| Bsp1407I | TGTACA | 1 cut(s) | 745 |
| BspACI | CCGC | 4 cut(s) | 120, 122, 153, 387 |
| BspANI | GGCC | 4 cut(s) | 55, 69, 84, 927 |
| BspCNI | CTCAG | 2 cut(s) | 286, 1282 |
| BspFNI | CGCG | 3 cut(s) | 122, 249, 513 |
| BspLI | GGNNCC | 3 cut(s) | 55, 56, 1248 |
| BspMI | ACCTGC | 1 cut(s) | 445 |
| BspPI | GGATC | 2 cut(s) | 903, 982 |
| BspT104I | TTCGAA | 1 cut(s) | 1030 |
| BspTNI | GGTCTC | 1 cut(s) | 849 |
| BsrGI | TGTACA | 1 cut(s) | 745 |
| BsrI | ACTGG | 1 cut(s) | 991 |
| BssECI | CCNNGG | 8 cut(s) | 120, 280, 707, 708, 709, 928, 1149, 1385 |
| BssNAI | GTATAC | 1 cut(s) | 984 |
| BssT1I | CCWWGG | 2 cut(s) | 928, 1149 |
| Bst1107I | GTATAC | 1 cut(s) | 984 |
| Bst2UI | CCWGG | 3 cut(s) | 972, 1217, 1386 |
| Bst4CI | ACNGT | 5 cut(s) | 25, 211, 281, 595, 816 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1061 |
| BstAUI | TGTACA | 1 cut(s) | 745 |
| BstBAI | YACGTR | 1 cut(s) | 1061 |
| BstBI | TTCGAA | 1 cut(s) | 1030 |
| BstC8I | GCNNGC | 4 cut(s) | 117, 389, 654, 839 |
| BstDEI | CTNAG | 2 cut(s) | 294, 1290 |
| BstDSI | CCRYGG | 2 cut(s) | 120, 280 |
| BstF5I | GGATG | 5 cut(s) | 42, 225, 331, 1322, 1429 |
| BstFNI | CGCG | 3 cut(s) | 122, 249, 513 |
| BstHHI | GCGC | 1 cut(s) | 513 |
| BstMAI | GTCTC | 4 cut(s) | 5, 582, 849, 1107 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 772, 1061, 1217 |
| BstNI | CCWGG | 3 cut(s) | 972, 1217, 1386 |
| BstNSI | RCATGY | 2 cut(s) | 841, 1068 |
| BstSCI | CCNGG | 6 cut(s) | 50, 707, 708, 970, 1215, 1384 |
| BstSFI | CTRYAG | 1 cut(s) | 1197 |
| BstSLI | GKGCMC | 1 cut(s) | 57 |
| BstUI | CGCG | 3 cut(s) | 122, 249, 513 |
| BstV1I | GCAGC | 2 cut(s) | 172, 244 |
| BstV2I | GAAGAC | 1 cut(s) | 23 |
| BstX2I | RGATCY | 3 cut(s) | 537, 974, 1092 |
| BstYI | RGATCY | 3 cut(s) | 537, 974, 1092 |
| BstZ17I | GTATAC | 1 cut(s) | 984 |
| Bsu36I | CCTNAGG | 2 cut(s) | 294, 1290 |
| BsuRI | GGCC | 4 cut(s) | 55, 69, 84, 927 |
| BtgI | CCRYGG | 2 cut(s) | 120, 280 |
| BtgZI | GCGATG | 1 cut(s) | 230 |
| BtsCI | GGATG | 5 cut(s) | 42, 225, 331, 1322, 1429 |
| BtsIMutI | CAGTG | 3 cut(s) | 207, 591, 845 |
| BveI | ACCTGC | 1 cut(s) | 445 |
| Cac8I | GCNNGC | 4 cut(s) | 117, 389, 654, 839 |
| CaiI | CAGNNNCTG | 2 cut(s) | 557, 977 |
| CfoI | GCGC | 1 cut(s) | 513 |
| Cfr13I | GGNCC | 3 cut(s) | 53, 54, 67 |
| Cfr42I | CCGCGG | 1 cut(s) | 123 |
| Cfr9I | CCCGGG | 1 cut(s) | 708 |
| CseI | GACGC | 5 cut(s) | 36, 238, 441, 502, 1154 |
| Csp6I | GTAC | 5 cut(s) | 189, 688, 746, 805, 1190 |
| CviQI | GTAC | 5 cut(s) | 189, 688, 746, 805, 1190 |
| DdeI | CTNAG | 2 cut(s) | 294, 1290 |
| DraIII | CACNNNGTG | 1 cut(s) | 213 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 512 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 512 |
| Eco130I | CCWWGG | 2 cut(s) | 928, 1149 |
| Eco24I | GRGCYC | 1 cut(s) | 57 |
| Eco31I | GGTCTC | 1 cut(s) | 849 |
| Eco57I | CTGAAG | 2 cut(s) | 401, 1326 |
| Eco81I | CCTNAGG | 2 cut(s) | 294, 1290 |
| Eco88I | CYCGRG | 2 cut(s) | 64, 708 |
| EcoO109I | RGGNCCY | 1 cut(s) | 54 |
| EcoRI | GAATTC | 1 cut(s) | 1296 |
| EcoRII | CCWGG | 3 cut(s) | 970, 1215, 1384 |
| EcoT14I | CCWWGG | 2 cut(s) | 928, 1149 |
| EcoT22I | ATGCAT | 3 cut(s) | 343, 843, 1057 |
| EcoT38I | GRGCYC | 1 cut(s) | 57 |
| ErhI | CCWWGG | 2 cut(s) | 928, 1149 |
| Esp3I | CGTCTC | 2 cut(s) | 5, 1107 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 149, 181 |
| FauI | CCCGC | 1 cut(s) | 380 |
| FbaI | TGATCA | 3 cut(s) | 222, 328, 699 |
| FblI | GTMKAC | 2 cut(s) | 546, 983 |
| Fnu4HI | GCNGC | 4 cut(s) | 120, 153, 161, 233 |
| FokI | GGATG | 4 cut(s) | 49, 212, 318, 1309 |
| FriOI | GRGCYC | 1 cut(s) | 57 |
| Fsp4HI | GCNGC | 4 cut(s) | 120, 153, 161, 233 |
| FspBI | CTAG | 2 cut(s) | 137, 1181 |
| GlaI | GCGC | 1 cut(s) | 512 |
| GluI | GCNGC | 4 cut(s) | 120, 153, 161, 233 |
| GsuI | CTGGAG | 1 cut(s) | 204 |
| HaeIII | GGCC | 4 cut(s) | 55, 69, 84, 927 |
| HapII | CCGG | 4 cut(s) | 34, 51, 198, 709 |
| HgaI | GACGC | 5 cut(s) | 36, 238, 441, 502, 1154 |
| HhaI | GCGC | 1 cut(s) | 513 |
| Hin6I | GCGC | 1 cut(s) | 511 |
| HinP1I | GCGC | 1 cut(s) | 511 |
| HincII | GTYRAC | 2 cut(s) | 28, 547 |
| HindII | GTYRAC | 2 cut(s) | 28, 547 |
| HindIII | AAGCTT | 1 cut(s) | 764 |
| HpaII | CCGG | 4 cut(s) | 34, 51, 198, 709 |
| HphI | GGTGA | 4 cut(s) | 469, 1049, 1406, 1415 |
| Hpy166II | GTNNAC | 6 cut(s) | 28, 547, 805, 819, 984, 1309 |
| Hpy8I | GTNNAC | 6 cut(s) | 28, 547, 805, 819, 984, 1309 |
| Hpy99I | CGWCG | 3 cut(s) | 83, 254, 435 |
| HpyAV | CCTTC | 3 cut(s) | 111, 175, 1234 |
| HpyCH4III | ACNGT | 5 cut(s) | 25, 211, 281, 595, 816 |
| HpyCH4IV | ACGT | 2 cut(s) | 807, 1060 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 341, 786, 841, 1002, 1055, 1064, 1071 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 772, 1061, 1217 |
| HpyF3I | CTNAG | 2 cut(s) | 294, 1290 |
| HpySE526I | ACGT | 2 cut(s) | 807, 1060 |
| HspAI | GCGC | 1 cut(s) | 511 |
| Ksp22I | TGATCA | 3 cut(s) | 222, 328, 699 |
| KspI | CCGCGG | 1 cut(s) | 123 |
| LmnI | GCTCC | 3 cut(s) | 919, 1149, 1291 |
| Lsp1109I | GCAGC | 2 cut(s) | 172, 244 |
| LweI | GCATC | 2 cut(s) | 328, 544 |
| MaeI | CTAG | 2 cut(s) | 137, 1181 |
| MaeII | ACGT | 2 cut(s) | 807, 1060 |
| MaeIII | GTNAC | 3 cut(s) | 1202, 1336, 1409 |
| MboII | GAAGA | 8 cut(s) | 28, 84, 87, 120, 547, 1102, 1342, 1379 |
| MfeI | CAATTG | 1 cut(s) | 1023 |
| MflI | RGATCY | 3 cut(s) | 537, 974, 1092 |
| MhlI | GDGCHC | 1 cut(s) | 57 |
| MlyI | GAGTC | 5 cut(s) | 69, 137, 308, 318, 611 |
| MmeI | TCCRAC | 2 cut(s) | 97, 171 |
| Mph1103I | ATGCAT | 3 cut(s) | 343, 843, 1057 |
| MroXI | GAANNNNTTC | 2 cut(s) | 369, 1371 |
| MseI | TTAA | 3 cut(s) | 420, 740, 1271 |
| MslI | CAYNNNNRTG | 2 cut(s) | 336, 1107 |
| MspA1I | CMGCKG | 1 cut(s) | 122 |
| MspI | CCGG | 4 cut(s) | 34, 51, 198, 709 |
| MspR9I | CCNGG | 6 cut(s) | 52, 709, 710, 972, 1217, 1386 |
| MunI | CAATTG | 1 cut(s) | 1023 |
| Mva1269I | GAATGC | 1 cut(s) | 466 |
| MvaI | CCWGG | 3 cut(s) | 972, 1217, 1386 |
| MvnI | CGCG | 3 cut(s) | 122, 249, 513 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 772, 1061, 1217 |
| NciI | CCSGG | 3 cut(s) | 52, 709, 710 |
| NlaIV | GGNNCC | 3 cut(s) | 55, 56, 1248 |
| NmuCI | GTSAC | 1 cut(s) | 1336 |
| NsiI | ATGCAT | 3 cut(s) | 343, 843, 1057 |
| NspI | RCATGY | 2 cut(s) | 841, 1068 |
| NspV | TTCGAA | 1 cut(s) | 1030 |
| PaeI | GCATGC | 1 cut(s) | 841 |
| PaqCI | CACCTGC | 1 cut(s) | 445 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 246, 904 |
| PctI | GAATGC | 1 cut(s) | 466 |
| PdmI | GAANNNNTTC | 2 cut(s) | 369, 1371 |
| PfeI | GAWTC | 6 cut(s) | 125, 269, 490, 850, 1099, 1253 |
| PkrI | GCNGC | 4 cut(s) | 121, 154, 162, 234 |
| PleI | GAGTC | 5 cut(s) | 69, 137, 307, 317, 611 |
| PpsI | GAGTC | 5 cut(s) | 69, 137, 307, 317, 611 |
| Ppu21I | YACGTR | 1 cut(s) | 1061 |
| Psp6I | CCWGG | 3 cut(s) | 970, 1215, 1384 |
| PspGI | CCWGG | 3 cut(s) | 970, 1215, 1384 |
| PspN4I | GGNNCC | 3 cut(s) | 55, 56, 1248 |
| PspOMI | GGGCCC | 1 cut(s) | 53 |
| PspPI | GGNCC | 3 cut(s) | 53, 54, 67 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1218, 1250 |
| PstNI | CAGNNNCTG | 2 cut(s) | 557, 977 |
| PsuI | RGATCY | 3 cut(s) | 537, 974, 1092 |
| RsaI | GTAC | 5 cut(s) | 190, 689, 747, 806, 1191 |
| RsaNI | GTAC | 5 cut(s) | 189, 688, 746, 805, 1190 |
| RseI | CAYNNNNRTG | 2 cut(s) | 336, 1107 |
| SacII | CCGCGG | 1 cut(s) | 123 |
| SalI | GTCGAC | 1 cut(s) | 545 |
| SaqAI | TTAA | 3 cut(s) | 420, 740, 1271 |
| SatI | GCNGC | 4 cut(s) | 120, 153, 161, 233 |
| Sau96I | GGNCC | 3 cut(s) | 53, 54, 67 |
| SchI | GAGTC | 5 cut(s) | 69, 137, 308, 318, 611 |
| ScrFI | CCNGG | 6 cut(s) | 52, 709, 710, 972, 1217, 1386 |
| SduI | GDGCHC | 1 cut(s) | 57 |
| SfaNI | GCATC | 2 cut(s) | 328, 544 |
| SfcI | CTRYAG | 1 cut(s) | 1197 |
| Sfr303I | CCGCGG | 1 cut(s) | 123 |
| SfuI | TTCGAA | 1 cut(s) | 1030 |
| SgrBI | CCGCGG | 1 cut(s) | 123 |
| SmaI | CCCGGG | 1 cut(s) | 710 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 336, 1107 |
| SmlI | CTYRAG | 1 cut(s) | 626 |
| SmoI | CTYRAG | 1 cut(s) | 626 |
| SphI | GCATGC | 1 cut(s) | 841 |
| SsiI | CCGC | 4 cut(s) | 120, 122, 153, 387 |
| SspI | AATATT | 1 cut(s) | 1266 |
| SspMI | CTAG | 2 cut(s) | 137, 1181 |
| StyD4I | CCNGG | 6 cut(s) | 50, 707, 708, 970, 1215, 1384 |
| StyI | CCWWGG | 2 cut(s) | 928, 1149 |
| TaaI | ACNGT | 5 cut(s) | 25, 211, 281, 595, 816 |
| TaiI | ACGT | 2 cut(s) | 810, 1063 |
| TaqI | TCGA | 5 cut(s) | 78, 430, 546, 853, 1030 |
| TaqII | GACCGA | 1 cut(s) | 873 |
| TatI | WGTACW | 2 cut(s) | 745, 1189 |
| TauI | GCSGC | 2 cut(s) | 122, 155 |
| TfiI | GAWTC | 6 cut(s) | 125, 269, 490, 850, 1099, 1253 |
| Tru1I | TTAA | 3 cut(s) | 420, 740, 1271 |
| Tru9I | TTAA | 3 cut(s) | 420, 740, 1271 |
| TscAI | CASTG | 3 cut(s) | 214, 598, 852 |
| TseFI | GTSAC | 1 cut(s) | 1336 |
| TseI | GCWGC | 2 cut(s) | 160, 232 |
| Tsp45I | GTSAC | 1 cut(s) | 1336 |
| TspDTI | ATGAA | 7 cut(s) | 93, 261, 542, 548, 1091, 1289, 1380 |
| TspGWI | ACGGA | 1 cut(s) | 506 |
| TspMI | CCCGGG | 1 cut(s) | 708 |
| TspRI | CASTG | 3 cut(s) | 214, 598, 852 |
| XapI | RAATTY | 3 cut(s) | 1296, 1370, 1397 |
| XbaI | TCTAGA | 1 cut(s) | 1180 |
| XceI | RCATGY | 2 cut(s) | 841, 1068 |
| XmaI | CCCGGG | 1 cut(s) | 708 |
| XmiI | GTMKAC | 2 cut(s) | 546, 983 |
| XmnI | GAANNNNTTC | 2 cut(s) | 369, 1371 |
| XspI | CTAG | 2 cut(s) | 137, 1181 |
| Zsp2I | ATGCAT | 3 cut(s) | 343, 843, 1057 |