Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000197

Physical Location & Seq

Genomic Coordinates Reverse (-)

23874863 .. 23877677

Gene Structure 2815 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1164000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGCTTCCAATGGAATCCGCTTCACTAGAGTAGAAGTAGAAGGCCTGTTACTGCCAGTGGGATACCGATTCCACCCCACAGAAGAAGAGCTGATCAGCCATTACTTGAAGTTGAAGCTCCGGGGAAAGGATTCCCTCGTCAGCGACGCCATCCGTGAAATCAATATCTGCAACTACGAGCCCAGGGAATTGCCTGAGATTTCACTGATAAAGTCGGATGATGAATGGTACTTCTACAACCGACCAATTTACAAAAAAAACAGCAGAAACGAAGTTGAAAGGGCGACGGCCACAGGCTTCTGGAAGAGCACCGGGAAGGAAAAAATAGTCAAGGCTCGAGACAACAAAATTATCGGTAAAAAGAGGATTCTGACATTTCGCCAAGGTCGCGGTCGTAATGCCCCAATTACCAACTGGGTCATGCATGAGTTTTCCATCCCTCAAATCACTCCTAATGCTAATCAGAGGGATTTCGTTCTCTGTTACGTAAAGAAGAAATCAGGTAAGAACACGGATGTTGCAACCGGTGATGAAGGCGAATCTAGTAATTACAACAATGCACCTGACTTTGAAAATCAACTGCAACCAGCACGTAATATGCATGTTGAAGAGGAACGTACTCAGCCACCACCAAATCCGGACATTTTTCAGAGGGAATTGGATCTTATGCTTGGAAATACGCGTGGTAATAATGATTACCATGGAATGGATGAAAATTTGGAAGAGTTCGTAAATGGACTTTTTGGGGATGACCCGTATCAGGGTTACAGTGAAGAAATAACTCACAATAACGTCTTTAACGACTCCACTCTGCCACAGTCACCTAGGAAGGTGTATTTACCGGATTGTGGAGTGAGCAGTGACTCAGACACAGAAGTACAACATCAAGCTGCACAGGGCCCGAGTTTACAAATGCTTGGTGTGCCACAAGCTAGTTCGAGCCACTTCGGAAGAGAGGAAGGTGTTATGCTTCGTAACCAGACCTTAGGACAGGCTGCTTCCTCTATCAATGTGTTACCTAAACCTCAGACTGATCGTCTTCAATCAATCAGTGATGAAGATTCTGGTACACACCAAATAACAAGCAGGCTGCGACGTGCATCAAGACCCATTAATGCCTTAAAATTAAAGAGTGCTTCCTCTGTGGATGTAGATGCAGTTAGCCTTCAGATTAACTCTATTCAGTTAGCCTCCGACGAATACTATAACAGTGAGAGAACACGCAGAAGAACATATCCAACAAGTGCCCTCTTAGCACTAGGCAAACCTAATGAATTAGAACAGCAGCAAAGCAAGGCCAAAGTAGCTGCAGAGCGTCGAGCTGCTGTTGATTTCCCCCCGAAGAGGAACTTTATAACAGAATCTAACAAAGACGCAGAGGCGGTTCAAGGCAATAATACAGAAAAGGGTTTGAAGCAGACACACAATGCAACCACCGCCGGCAATTGGATGAGCTGTTCTTGCATTTCCGGGGAAACTTCCCCCCCATTAACAGGTCCCCCATCAGTATACCTTTTCAACACGGTTCTACTTCTAGGTGCGATATTGTTCTTTGTTTTTGCTTGGGAAGAAGGTTTATACGGGCAGTGGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071944 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

532

Amino Acids

59.7

Weight (kDa)

6.02

Isoelectric Point (pI)

47.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	19 - 146	1.9e-34	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000197)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g18010 FvH4_7g18010 FvH4_7g18021 FvH4_7g18070 FvH4_7g18070 FvH4_7g18200 FvH4_7g18250
malus_domestica	MD01G1092200.v1.1 MD01G1092500.v1.1 MD01G1092600.v1.1 MD01G1092900.v1.1 MD01G1093000.v1.1 MD01G1093200.v1.1 MD01G1093500.v1.1 MD01G1093700.v1.1 MD01G1093800.v1.1 MD01G1093900.v1.1 MD01G1094000.v1.1 MD01G1094200.v1.1 MD01G1094400.v1.1 MD01G1094500.v1.1 MD01G1094700.v1.1 MD01G1094800.v1.1 MD01G1095100.v1.1 MD07G1162700.v1.1 MD07G1163200.v1.1 MD07G1163400.v1.1 MD07G1163700.v1.1 MD07G1164000.v1.1 MD10G1186100.v1.1
prunus_persica	Prupe.2G201200_v2.0.a1 Prupe.2G201200_v2.0.a1 Prupe.2G201300_v2.0.a1 Prupe.2G201800_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202800_v2.0.a1 Prupe.2G202900_v2.0.a1 Prupe.2G203000_v2.0.a1 Prupe.2G204700_v2.0.a1 Prupe.2G204700_v2.0.a1 Prupe.2G204900_v2.0.a1
pyrus_communis	pycom01g11610 pycom01g11650 pycom01g11670 pycom01g11680 pycom01g11700 pycom01g11710 pycom01g11720 pycom01g11730 pycom07g15770 pycom07g15860 pycom07g15870 pycom07g15890 pycom07g15900 pycom07g15920
rosa_chinensis	RchiOBHm_Chr1g0333731 RchiOBHm_Chr1g0361411 RchiOBHm_Chr1g0361501 RchiOBHm_Chr1g0361551 RchiOBHm_Chr1g0361631 RchiOBHm_Chr1g0361691 RchiOBHm_Chr1g0361701
rosa_laevigata	RLG00000011640 RLG00000014882 RLG00000027709 RLG00000027713 RLG00000027717 RLG00000027719 RLG00000029532 RLG00000030171 RLG00000030174 RLG00000030177 RLG00000030181 RLG00000030186
rosa_multiflora	Rmu_co8135790.1_g000001 Rmu_co8371689.1_g000001 Rmu_co8391943.1_g000001 Rmu_co8469521.1_g000001 Rmu_co8502503.1_g000002 Rmu_sc0001034.1_g000001 Rmu_sc0002119.1_g000002 Rmu_sc0002119.1_g000009 Rmu_sc0002119.1_g000019 Rmu_sc0003426.1_g000005 Rmu_sc0003693.1_g000010 Rmu_sc0003704.1_g000007 Rmu_sc0006101.1_g000005 Rmu_sc0006417.1_g000006 Rmu_sc0007217.1_g000012 Rmu_sc0010384.1_g000001 Rmu_sc0011258.1_g000003 Rmu_sc0017253.1_g000002 Rmu_ssc0000062.1_g000022
rosa_roxburghii	Rroxscaffold_159G00432930 Rroxscaffold_159G00433040 Rroxscaffold_159G00433060 Rroxscaffold_4G00294780 Rroxscaffold_4G00294810 Rroxscaffold_4G00294870 Rroxscaffold_4G00294930 Rroxscaffold_4G00294950 Rroxscaffold_4G00317240 Rroxscaffold_4G00325190 Rroxscaffold_4G00325210
rosa_rugosa	Rorug01G0047400 Rorug01G0047800 Rorug01G0048100 Rorug01G0048100 Rorug01G0111500 Rorug01G0111500 Rorug01G0289800 Rorug01G0290300 Rorug01G0290700 Rorug01G0290900
rosa_samantha	Rh1AG063000 Rh1AG064000 Rh1AG064400 Rh1AG133700 Rh1AG300300 Rh1AG300900 Rh1AG301000 Rh1AG301100 Rh1BG263800 Rh1BG264000 Rh1BG264100 Rh1CG281800 Rh1CG282100 Rh1CG282200 Rh1DG294000 Rh1DG294100 Rh5BG200600
rosa_wichuraiana	Rw1G005400 Rw1G011170 Rw1G026570 Rw1G026580 Rw1G026610 Rw1G026640 Rw1G026670 Rw1G026700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1355
AccB7I	CCANNNNNTGG	1 cut(s)	706
AccI	GTMKAC	1 cut(s)	1509
AccII	CGCG	2 cut(s)	390, 682
AccIII	TCCGGA	1 cut(s)	637
AciI	CCGC	4 cut(s)	19, 390, 1382, 1437
AclWI	GGATC	1 cut(s)	669
AcoI	YGGCCR	1 cut(s)	288
AcsI	RAATTY	1 cut(s)	715
AcuI	CTGAAG	1 cut(s)	1151
AcyI	GRCGYC	1 cut(s)	147
AfaI	GTAC	4 cut(s)	230, 619, 879, 1069
AfiI	CCNNNNNNNGG	8 cut(s)	127, 706, 760, 761, 848, 1344, 1446, 1493
AflIII	ACRYGT	1 cut(s)	680
AgeI	ACCGGT	1 cut(s)	524
AgsI	TTSAA	9 cut(s)	109, 115, 278, 572, 608, 1043, 1388, 1414, 1519
AhdI	GACNNNNNGTC	1 cut(s)	1035
AjiI	CACGTC	1 cut(s)	1097
AjnI	CCWGG	1 cut(s)	182
AloI	GAACNNNNNNTCC	2 cut(s)	1441, 1473
AluBI	AGCT	7 cut(s)	91, 118, 890, 932, 1307, 1322, 1455
AluI	AGCT	7 cut(s)	91, 118, 890, 932, 1307, 1322, 1455
Alw21I	GWGCWC	1 cut(s)	311
Alw26I	GTCTC	1 cut(s)	333
AlwI	GGATC	1 cut(s)	669
Ama87I	CYCGRG	2 cut(s)	336, 901
Aor13HI	TCCGGA	1 cut(s)	637
AoxI	GGCC	4 cut(s)	43, 288, 898, 1296
ApaI	GGGCCC	1 cut(s)	902
ApeKI	GCWGC	6 cut(s)	890, 995, 1090, 1285, 1307, 1322
ApoI	RAATTY	1 cut(s)	715
AseI	ATTAAT	1 cut(s)	1113
AsiGI	ACCGGT	1 cut(s)	524
Asp700I	GAANNNNTTC	1 cut(s)	130
AspA2I	CCTAGG	1 cut(s)	824
AspS9I	GGNCC	3 cut(s)	898, 899, 1496
AsuC2I	CCSGG	3 cut(s)	122, 313, 1471
AsuHPI	GGTGA	2 cut(s)	539, 813
AvaI	CYCGRG	2 cut(s)	336, 901
AvaII	GGWCC	1 cut(s)	1496
AvrII	CCTAGG	1 cut(s)	824
AxyI	CCTNAGG	1 cut(s)	985
BaeGI	GKGCMC	2 cut(s)	902, 1249
BanII	GRGCYC	2 cut(s)	183, 902
BarI	GAAGNNNNNNTAC	2 cut(s)	33, 65
BbsI	GAAGAC	1 cut(s)	1031
Bbv12I	GWGCWC	1 cut(s)	311
BbvI	GCAGC	6 cut(s)	877, 982, 1077, 1294, 1297, 1309
BccI	CCATC	3 cut(s)	158, 443, 1510
BceAI	ACGGC	1 cut(s)	303
BciT130I	CCWGG	1 cut(s)	184
BciVI	GTATCC	1 cut(s)	56
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	3 cut(s)	122, 313, 1471
BcoDI	GTCTC	1 cut(s)	333
BfaI	CTAG	7 cut(s)	27, 543, 825, 933, 1259, 1535, 1594
BfmI	CTRYAG	1 cut(s)	1308
BfuI	GTATCC	1 cut(s)	56
BisI	GCNGC	6 cut(s)	891, 996, 1091, 1286, 1308, 1323
BlnI	CCTAGG	1 cut(s)	824
BlsI	GCNGC	6 cut(s)	892, 997, 1092, 1287, 1309, 1324
Bme1390I	CCNGG	4 cut(s)	122, 184, 313, 1471
Bme18I	GGWCC	1 cut(s)	1496
BmeRI	GACNNNNNGTC	1 cut(s)	1035
BmeT110I	CYCGRG	2 cut(s)	336, 901
BmgBI	CACGTC	1 cut(s)	1097
BmgT120I	GGNCC	3 cut(s)	898, 899, 1496
BmiI	GGNNCC	2 cut(s)	900, 1498
BmrFI	CCNGG	4 cut(s)	122, 184, 313, 1471
BmrI	ACTGGG	1 cut(s)	424
BmsI	GCATC	2 cut(s)	1109, 1144
BmuI	ACTGGG	1 cut(s)	424
BpiI	GAAGAC	1 cut(s)	1031
BpuMI	CCSGG	3 cut(s)	122, 313, 1471
BsaAI	YACGTR	2 cut(s)	487, 593
BsaHI	GRCGYC	1 cut(s)	147
BsaJI	CCNNGG	7 cut(s)	121, 182, 183, 382, 700, 824, 1470
BsaWI	WCCGGW	3 cut(s)	524, 637, 841
Bsc4I	CCNNNNNNNGG	8 cut(s)	127, 706, 760, 761, 848, 1344, 1446, 1493
Bse118I	RCCGGY	2 cut(s)	524, 1439
Bse1I	ACTGG	2 cut(s)	56, 419
Bse21I	CCTNAGG	1 cut(s)	985
BseAI	TCCGGA	1 cut(s)	637
BseBI	CCWGG	1 cut(s)	184
BseDI	CCNNGG	7 cut(s)	121, 182, 183, 382, 700, 824, 1470
BseGI	GGATG	8 cut(s)	150, 223, 435, 520, 715, 754, 1153, 1455
BseLI	CCNNNNNNNGG	8 cut(s)	127, 706, 760, 761, 848, 1344, 1446, 1493
BseMII	CTCAG	4 cut(s)	186, 635, 879, 1040
BseNI	ACTGG	2 cut(s)	56, 419
BseSI	GKGCMC	2 cut(s)	902, 1249
BseXI	GCAGC	6 cut(s)	877, 982, 1077, 1294, 1297, 1309
BsgI	GTGCAG	1 cut(s)	876
Bsh1236I	CGCG	2 cut(s)	390, 682
Bsh1285I	CGRYCG	1 cut(s)	394
BshFI	GGCC	4 cut(s)	45, 290, 900, 1298
BshTI	ACCGGT	1 cut(s)	524
BsiEI	CGRYCG	1 cut(s)	394
BsiHKAI	GWGCWC	1 cut(s)	311
BsiHKCI	CYCGRG	2 cut(s)	336, 901
BsiSI	CCGG	7 cut(s)	121, 312, 525, 638, 842, 1440, 1470
BslFI	GGGAC	1 cut(s)	1482
BslI	CCNNNNNNNGG	8 cut(s)	127, 706, 760, 761, 848, 1344, 1446, 1493
BsmAI	GTCTC	1 cut(s)	333
BsmFI	GGGAC	1 cut(s)	1482
BsnI	GGCC	4 cut(s)	45, 290, 900, 1298
BsoBI	CYCGRG	2 cut(s)	336, 901
Bsp120I	GGGCCC	1 cut(s)	898
Bsp1286I	GDGCHC	4 cut(s)	183, 311, 902, 1249
Bsp13I	TCCGGA	1 cut(s)	637
Bsp143I	GATC	3 cut(s)	93, 661, 1033
Bsp19I	CCATGG	1 cut(s)	700
BspACI	CCGC	4 cut(s)	19, 390, 1382, 1437
BspANI	GGCC	4 cut(s)	45, 290, 900, 1298
BspCNI	CTCAG	4 cut(s)	187, 634, 878, 1039
BspEI	TCCGGA	1 cut(s)	637
BspFNI	CGCG	2 cut(s)	390, 682
BspLI	GGNNCC	2 cut(s)	900, 1498
BspMAI	CTGCAG	1 cut(s)	1312
BspPI	GGATC	1 cut(s)	669
BspQI	GCTCTTC	2 cut(s)	81, 299
BsrFI	RCCGGY	2 cut(s)	524, 1439
BsrI	ACTGG	2 cut(s)	56, 419
BssAI	RCCGGY	2 cut(s)	524, 1439
BssECI	CCNNGG	7 cut(s)	121, 182, 183, 382, 700, 824, 1470
BssMI	GATC	3 cut(s)	93, 661, 1033
BssNAI	GTATAC	1 cut(s)	1510
BssNI	GRCGYC	1 cut(s)	147
BssT1I	CCWWGG	3 cut(s)	382, 700, 824
Bst1107I	GTATAC	1 cut(s)	1510
Bst2UI	CCWGG	1 cut(s)	184
Bst4CI	ACNGT	4 cut(s)	770, 819, 1211, 1525
Bst6I	CTCTTC	6 cut(s)	81, 299, 603, 717, 946, 1337
BstACI	GRCGYC	1 cut(s)	147
BstBAI	YACGTR	2 cut(s)	487, 593
BstC8I	GCNNGC	2 cut(s)	1088, 1441
BstDEI	CTNAG	6 cut(s)	195, 621, 865, 985, 1026, 1252
BstDSI	CCRYGG	1 cut(s)	700
BstF5I	GGATG	8 cut(s)	150, 223, 435, 520, 715, 754, 1153, 1455
BstFNI	CGCG	2 cut(s)	390, 682
BstKTI	GATC	3 cut(s)	96, 664, 1036
BstMAI	GTCTC	1 cut(s)	333
BstMBI	GATC	3 cut(s)	93, 661, 1033
BstMCI	CGRYCG	1 cut(s)	394
BstMWI	GCNNNNNNNGC	6 cut(s)	387, 922, 1253, 1304, 1436, 1461
BstNI	CCWGG	1 cut(s)	184
BstNSI	RCATGY	1 cut(s)	605
BstSCI	CCNGG	4 cut(s)	120, 182, 311, 1469
BstSFI	CTRYAG	1 cut(s)	1308
BstSLI	GKGCMC	2 cut(s)	902, 1249
BstSNI	TACGTA	1 cut(s)	487
BstUI	CGCG	2 cut(s)	390, 682
BstV1I	GCAGC	6 cut(s)	877, 982, 1077, 1294, 1297, 1309
BstV2I	GAAGAC	1 cut(s)	1031
BstX2I	RGATCY	1 cut(s)	661
BstYI	RGATCY	1 cut(s)	661
BstZ17I	GTATAC	1 cut(s)	1510
Bsu36I	CCTNAGG	1 cut(s)	985
BsuI	GTATCC	1 cut(s)	56
BsuRI	GGCC	4 cut(s)	45, 290, 900, 1298
BtgI	CCRYGG	1 cut(s)	700
BtrI	CACGTC	1 cut(s)	1097
BtsCI	GGATG	8 cut(s)	150, 223, 435, 520, 715, 754, 1153, 1455
BtsI	GCAGTG	2 cut(s)	865, 1592
BtsIMutI	CAGTG	7 cut(s)	63, 203, 775, 865, 1057, 1216, 1592
Cac8I	GCNNGC	2 cut(s)	1088, 1441
Cfr10I	RCCGGY	2 cut(s)	524, 1439
Cfr13I	GGNCC	3 cut(s)	898, 899, 1496
CseI	GACGC	3 cut(s)	155, 1304, 1382
Csp6I	GTAC	4 cut(s)	229, 618, 878, 1068
CspAI	ACCGGT	1 cut(s)	524
CviAII	CATG	4 cut(s)	421, 425, 602, 701
CviQI	GTAC	4 cut(s)	229, 618, 878, 1068
DdeI	CTNAG	6 cut(s)	195, 621, 865, 985, 1026, 1252
DpnI	GATC	3 cut(s)	95, 663, 1035
DpnII	GATC	3 cut(s)	93, 661, 1033
DriI	GACNNNNNGTC	1 cut(s)	1035
EaeI	YGGCCR	1 cut(s)	288
Eam1104I	CTCTTC	6 cut(s)	81, 299, 603, 717, 946, 1337
Eam1105I	GACNNNNNGTC	1 cut(s)	1035
EarI	CTCTTC	6 cut(s)	81, 299, 603, 717, 946, 1337
Eco105I	TACGTA	1 cut(s)	487
Eco130I	CCWWGG	3 cut(s)	382, 700, 824
Eco147I	AGGCCT	1 cut(s)	45
Eco24I	GRGCYC	2 cut(s)	183, 902
Eco47I	GGWCC	1 cut(s)	1496
Eco57I	CTGAAG	1 cut(s)	1151
Eco81I	CCTNAGG	1 cut(s)	985
Eco88I	CYCGRG	2 cut(s)	336, 901
EcoO109I	RGGNCCY	2 cut(s)	898, 1496
EcoRII	CCWGG	1 cut(s)	182
EcoT14I	CCWWGG	3 cut(s)	382, 700, 824
EcoT22I	ATGCAT	2 cut(s)	426, 603
EcoT38I	GRGCYC	2 cut(s)	183, 902
ErhI	CCWWGG	3 cut(s)	382, 700, 824
FaeI	CATG	4 cut(s)	424, 428, 605, 704
FalI	AAGNNNNNCTT	2 cut(s)	1334, 1366
FaqI	GGGAC	1 cut(s)	1482
FatI	CATG	4 cut(s)	420, 424, 601, 700
FbaI	TGATCA	1 cut(s)	93
FblI	GTMKAC	1 cut(s)	1509
Fnu4HI	GCNGC	6 cut(s)	891, 996, 1091, 1286, 1308, 1323
FokI	GGATG	8 cut(s)	137, 230, 422, 527, 722, 761, 1160, 1462
FriOI	GRGCYC	2 cut(s)	183, 902
Fsp4HI	GCNGC	6 cut(s)	891, 996, 1091, 1286, 1308, 1323
FspBI	CTAG	7 cut(s)	27, 543, 825, 933, 1259, 1535, 1594
GluI	GCNGC	6 cut(s)	891, 996, 1091, 1286, 1308, 1323
HaeIII	GGCC	4 cut(s)	45, 290, 900, 1298
HapII	CCGG	7 cut(s)	121, 312, 525, 638, 842, 1440, 1470
HgaI	GACGC	3 cut(s)	155, 1304, 1382
Hin1I	GRCGYC	1 cut(s)	147
Hin1II	CATG	4 cut(s)	424, 428, 605, 704
HinfI	GANTC	9 cut(s)	15, 69, 131, 367, 539, 803, 863, 1061, 1361
HpaII	CCGG	7 cut(s)	121, 312, 525, 638, 842, 1440, 1470
HphI	GGTGA	2 cut(s)	539, 813
Hpy166II	GTNNAC	3 cut(s)	908, 1070, 1510
Hpy188I	TCNGA	9 cut(s)	217, 372, 465, 651, 868, 950, 1029, 1170, 1195
Hpy188III	TCNNGA	4 cut(s)	301, 338, 638, 1104
Hpy8I	GTNNAC	3 cut(s)	908, 1070, 1510
Hpy99I	CGWCG	5 cut(s)	149, 289, 1098, 1199, 1320
HpyAV	CCTTC	7 cut(s)	35, 310, 527, 823, 953, 1175, 1565
HpyCH4III	ACNGT	4 cut(s)	770, 819, 1211, 1525
HpyCH4IV	ACGT	5 cut(s)	486, 592, 616, 792, 1096
HpyF10VI	GCNNNNNNNGC	6 cut(s)	387, 922, 1253, 1304, 1436, 1461
HpyF3I	CTNAG	6 cut(s)	195, 621, 865, 985, 1026, 1252
HpySE526I	ACGT	5 cut(s)	486, 592, 616, 792, 1096
Hsp92I	GRCGYC	1 cut(s)	147
Hsp92II	CATG	4 cut(s)	424, 428, 605, 704
Kpn2I	TCCGGA	1 cut(s)	637
KroI	GCCGGC	1 cut(s)	1439
KroNI	GCCGGC	1 cut(s)	1441
Ksp22I	TGATCA	1 cut(s)	93
Kzo9I	GATC	3 cut(s)	93, 661, 1033
LguI	GCTCTTC	2 cut(s)	81, 299
LmnI	GCTCC	1 cut(s)	123
Lsp1109I	GCAGC	6 cut(s)	877, 982, 1077, 1294, 1297, 1309
LweI	GCATC	2 cut(s)	1109, 1144
MaeI	CTAG	7 cut(s)	27, 543, 825, 933, 1259, 1535, 1594
MaeII	ACGT	5 cut(s)	486, 592, 616, 792, 1096
MaeIII	GTNAC	7 cut(s)	48, 482, 764, 819, 860, 974, 1014
MalI	GATC	3 cut(s)	95, 663, 1035
MboI	GATC	3 cut(s)	93, 661, 1033
MfeI	CAATTG	1 cut(s)	1444
MflI	RGATCY	1 cut(s)	661
MhlI	GDGCHC	4 cut(s)	183, 311, 902, 1249
MluI	ACGCGT	1 cut(s)	680
MlyI	GAGTC	2 cut(s)	797, 857
MmeI	TCCRAC	3 cut(s)	195, 1218, 1262
Mph1103I	ATGCAT	2 cut(s)	426, 603
MroI	TCCGGA	1 cut(s)	637
MroNI	GCCGGC	1 cut(s)	1439
MroXI	GAANNNNTTC	1 cut(s)	130
MseI	TTAA	6 cut(s)	798, 1113, 1121, 1127, 1173, 1490
MspI	CCGG	7 cut(s)	121, 312, 525, 638, 842, 1440, 1470
MspR9I	CCNGG	4 cut(s)	122, 184, 313, 1471
MunI	CAATTG	1 cut(s)	1444
MvaI	CCWGG	1 cut(s)	184
MvnI	CGCG	2 cut(s)	390, 682
MwoI	GCNNNNNNNGC	6 cut(s)	387, 922, 1253, 1304, 1436, 1461
NaeI	GCCGGC	1 cut(s)	1441
NciI	CCSGG	3 cut(s)	122, 313, 1471
NcoI	CCATGG	1 cut(s)	700
NdeII	GATC	3 cut(s)	93, 661, 1033
NgoMIV	GCCGGC	1 cut(s)	1439
NlaIII	CATG	4 cut(s)	424, 428, 605, 704
NlaIV	GGNNCC	2 cut(s)	900, 1498
NmuCI	GTSAC	2 cut(s)	819, 860
NsiI	ATGCAT	2 cut(s)	426, 603
NspI	RCATGY	1 cut(s)	605
PaeR7I	CTCGAG	1 cut(s)	336
PasI	CCCWGGG	1 cut(s)	183
PceI	AGGCCT	1 cut(s)	45
PciSI	GCTCTTC	2 cut(s)	81, 299
PcsI	WCGNNNNNNNCGW	1 cut(s)	798
PdiI	GCCGGC	1 cut(s)	1441
PdmI	GAANNNNTTC	1 cut(s)	130
PfeI	GAWTC	7 cut(s)	15, 69, 131, 367, 539, 1061, 1361
PflMI	CCANNNNNTGG	1 cut(s)	706
PinAI	ACCGGT	1 cut(s)	524
PkrI	GCNGC	6 cut(s)	892, 997, 1092, 1287, 1309, 1324
PleI	GAGTC	2 cut(s)	797, 857
PpsI	GAGTC	2 cut(s)	797, 857
Ppu21I	YACGTR	2 cut(s)	487, 593
PpuMI	RGGWCCY	1 cut(s)	1496
PshBI	ATTAAT	1 cut(s)	1113
PsiI	TTATAA	1 cut(s)	1355
Psp5II	RGGWCCY	1 cut(s)	1496
Psp6I	CCWGG	1 cut(s)	182
PspGI	CCWGG	1 cut(s)	182
PspN4I	GGNNCC	2 cut(s)	900, 1498
PspOMI	GGGCCC	1 cut(s)	898
PspPI	GGNCC	3 cut(s)	898, 899, 1496
PspPPI	RGGWCCY	1 cut(s)	1496
PstI	CTGCAG	1 cut(s)	1312
PsuI	RGATCY	1 cut(s)	661
RsaI	GTAC	4 cut(s)	230, 619, 879, 1069
RsaNI	GTAC	4 cut(s)	229, 618, 878, 1068
SapI	GCTCTTC	2 cut(s)	81, 299
SaqAI	TTAA	6 cut(s)	798, 1113, 1121, 1127, 1173, 1490
SatI	GCNGC	6 cut(s)	891, 996, 1091, 1286, 1308, 1323
Sau3AI	GATC	3 cut(s)	93, 661, 1033
Sau96I	GGNCC	3 cut(s)	898, 899, 1496
SchI	GAGTC	2 cut(s)	797, 857
ScrFI	CCNGG	4 cut(s)	122, 184, 313, 1471
SduI	GDGCHC	4 cut(s)	183, 311, 902, 1249
SfaNI	GCATC	2 cut(s)	1109, 1144
SfcI	CTRYAG	1 cut(s)	1308
Sfr274I	CTCGAG	1 cut(s)	336
SinI	GGWCC	1 cut(s)	1496
SlaI	CTCGAG	1 cut(s)	336
SmlI	CTYRAG	1 cut(s)	336
SmoI	CTYRAG	1 cut(s)	336
SnaBI	TACGTA	1 cut(s)	487
SseBI	AGGCCT	1 cut(s)	45
SsiI	CCGC	4 cut(s)	19, 390, 1382, 1437
SspMI	CTAG	7 cut(s)	27, 543, 825, 933, 1259, 1535, 1594
StuI	AGGCCT	1 cut(s)	45
StyD4I	CCNGG	4 cut(s)	120, 182, 311, 1469
StyI	CCWWGG	3 cut(s)	382, 700, 824
TaaI	ACNGT	4 cut(s)	770, 819, 1211, 1525
TaiI	ACGT	5 cut(s)	489, 595, 619, 795, 1099
TaqI	TCGA	3 cut(s)	337, 938, 1318
TatI	WGTACW	1 cut(s)	877
TfiI	GAWTC	7 cut(s)	15, 69, 131, 367, 539, 1061, 1361
Tru1I	TTAA	6 cut(s)	798, 1113, 1121, 1127, 1173, 1490
Tru9I	TTAA	6 cut(s)	798, 1113, 1121, 1127, 1173, 1490
TscAI	CASTG	7 cut(s)	63, 210, 775, 865, 1057, 1216, 1592
TseFI	GTSAC	2 cut(s)	819, 860
TseI	GCWGC	6 cut(s)	890, 995, 1090, 1285, 1307, 1322
Tsp45I	GTSAC	2 cut(s)	819, 860
TspDTI	ATGAA	5 cut(s)	237, 546, 726, 1071, 1287
TspGWI	ACGGA	2 cut(s)	143, 527
TspRI	CASTG	7 cut(s)	63, 210, 775, 865, 1057, 1216, 1592
Van91I	CCANNNNNTGG	1 cut(s)	706
VpaK11BI	GGWCC	1 cut(s)	1496
VspI	ATTAAT	1 cut(s)	1113
XapI	RAATTY	1 cut(s)	715
XceI	RCATGY	1 cut(s)	605
XcmI	CCANNNNNNNNNTGG	1 cut(s)	411
XhoI	CTCGAG	1 cut(s)	336
XmaJI	CCTAGG	1 cut(s)	824
XmiI	GTMKAC	1 cut(s)	1509
XmnI	GAANNNNTTC	1 cut(s)	130
XspI	CTAG	7 cut(s)	27, 543, 825, 933, 1259, 1535, 1594
Zsp2I	ATGCAT	2 cut(s)	426, 603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.