Rorug01G0048100
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000197
Physical Location & Seq
Forward (+)
7935519 .. 7947183
11665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0048100.1

Sequence Viewer

Length: 2226 bp
ATGATGTACCCTGATCCTCAACAGCAGCAGCAACACCAACAACAGCAGCAGCAGCAACACCAACAACAACAGCAGCACTATCACCAGCAGCAACAGCAACACCACCAACACCAGCAGCAACACCATCAGCATCAGCAGCAAGGTGGAGACTTTCACAGGGGCCCACCGCCGCAGCAGCCGCCGCCGATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGGCTCCGGATTATCACCACCCCTCCGCCCCTGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTCGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCTGCAATTGACATGTTGCCGACAGTTGCCTATTCAGATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTGAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCAGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATTTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTACGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGACACTCCCCAGGTTGAAATTGCTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGACTCAAGGTTATGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGGCCACCAACTCCAGGACCATTTACTCCCGGGGGAACTCGAAATGAAGGAACCATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATACCATCCCTTGACGCATCTATTCAGGGAGAACAACAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCACCTGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAAATGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCTGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

741

Amino Acids

82.65

Weight (kDa)

8.79

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 125 - 412 2e-14 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 159 - 339 2.2e-25 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 167 - 245 2.7e-06 WDR3 second beta-propeller domain
WDR55 PF24796 169 - 246 8.7e-06 WDR55
Beta-prop_WDR3_1st PF25173 170 - 322 6.8e-23 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 172 - 348 1.4e-27 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 173 - 248 8e-08 WDR5 beta-propeller domain
Beta-prop_IFT140_1st PF23383 176 - 262 2.8e-09 IFT140 first beta-propeller
WD40_Gbeta PF25391 177 - 380 3.7e-20 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 185 - 251 2.5e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 186 - 379 2.4e-31 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 208 - 323 2.5e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 210 - 243 1e-05 WD domain, G-beta repeat
EIF3I PF24805 211 - 293 1.5e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 220 - 390 1.3e-14 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 233 - 338 1.2e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 239 - 329 7.3e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 239 - 354 2.1e-23 WDR5 beta-propeller domain
WDR55 PF24796 248 - 411 5.9e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 252 - 412 2.2e-18 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 268 - 435 3.6e-20 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 272 - 360 5.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_IFT140_1st PF23383 282 - 367 3.9e-06 IFT140 first beta-propeller
Beta-prop_WDR3_1st PF25173 285 - 412 2.5e-28 WDR3 first beta-propeller domain
WD40 PF00400 292 - 328 4.4e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 294 - 376 1.3e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 295 - 454 1.2e-13 EIF3I
Beta-prop_WDR3_2nd PF25172 295 - 412 1.2e-13 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 296 - 402 4e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 302 - 412 1.6e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 306 - 453 1.4e-07 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 323 - 456 1.8e-10 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 333 - 457 6.8e-22 CDC20/Fizzy WD40 domain
WD40 PF00400 333 - 370 5.9e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 339 - 456 2.2e-20 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 340 - 393 8.7e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 341 - 458 2.8e-22 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 341 - 409 2.1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 345 - 422 2.9e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 377 - 455 4.6e-10 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000197)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18010 FvH4_7g18010 FvH4_7g18021 FvH4_7g18070 FvH4_7g18070 FvH4_7g18200 FvH4_7g18250
malus_domestica MD01G1092200.v1.1 MD01G1092500.v1.1 MD01G1092600.v1.1 MD01G1092900.v1.1 MD01G1093000.v1.1 MD01G1093200.v1.1 MD01G1093500.v1.1 MD01G1093700.v1.1 MD01G1093800.v1.1 MD01G1093900.v1.1 MD01G1094000.v1.1 MD01G1094200.v1.1 MD01G1094400.v1.1 MD01G1094500.v1.1 MD01G1094700.v1.1 MD01G1094800.v1.1 MD01G1095100.v1.1 MD07G1162700.v1.1 MD07G1163200.v1.1 MD07G1163400.v1.1 MD07G1163700.v1.1 MD07G1164000.v1.1 MD10G1186100.v1.1
prunus_persica Prupe.2G201200_v2.0.a1 Prupe.2G201200_v2.0.a1 Prupe.2G201300_v2.0.a1 Prupe.2G201800_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202000_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202500_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202700_v2.0.a1 Prupe.2G202800_v2.0.a1 Prupe.2G202900_v2.0.a1 Prupe.2G203000_v2.0.a1 Prupe.2G204700_v2.0.a1 Prupe.2G204700_v2.0.a1 Prupe.2G204900_v2.0.a1
pyrus_communis pycom01g11610 pycom01g11650 pycom01g11670 pycom01g11680 pycom01g11700 pycom01g11710 pycom01g11720 pycom01g11730 pycom07g15770 pycom07g15860 pycom07g15870 pycom07g15890 pycom07g15900 pycom07g15920
rosa_chinensis RchiOBHm_Chr1g0333731 RchiOBHm_Chr1g0361411 RchiOBHm_Chr1g0361501 RchiOBHm_Chr1g0361551 RchiOBHm_Chr1g0361631 RchiOBHm_Chr1g0361691 RchiOBHm_Chr1g0361701
rosa_laevigata RLG00000011640 RLG00000014882 RLG00000027709 RLG00000027713 RLG00000027717 RLG00000027719 RLG00000029532 RLG00000030171 RLG00000030174 RLG00000030177 RLG00000030181 RLG00000030186
rosa_multiflora Rmu_co8135790.1_g000001 Rmu_co8371689.1_g000001 Rmu_co8391943.1_g000001 Rmu_co8469521.1_g000001 Rmu_co8502503.1_g000002 Rmu_sc0001034.1_g000001 Rmu_sc0002119.1_g000002 Rmu_sc0002119.1_g000009 Rmu_sc0002119.1_g000019 Rmu_sc0003426.1_g000005 Rmu_sc0003693.1_g000010 Rmu_sc0003704.1_g000007 Rmu_sc0006101.1_g000005 Rmu_sc0006417.1_g000006 Rmu_sc0007217.1_g000012 Rmu_sc0010384.1_g000001 Rmu_sc0011258.1_g000003 Rmu_sc0017253.1_g000002 Rmu_ssc0000062.1_g000022
rosa_roxburghii Rroxscaffold_159G00432930 Rroxscaffold_159G00433040 Rroxscaffold_159G00433060 Rroxscaffold_4G00294780 Rroxscaffold_4G00294810 Rroxscaffold_4G00294870 Rroxscaffold_4G00294930 Rroxscaffold_4G00294950 Rroxscaffold_4G00317240 Rroxscaffold_4G00325190 Rroxscaffold_4G00325210
rosa_rugosa Rorug01G0047400 Rorug01G0047800 Rorug01G0048100 Rorug01G0048100 Rorug01G0111500 Rorug01G0111500 Rorug01G0289800 Rorug01G0290300 Rorug01G0290700 Rorug01G0290900
rosa_samantha Rh1AG063000 Rh1AG064000 Rh1AG064400 Rh1AG133700 Rh1AG300300 Rh1AG300900 Rh1AG301000 Rh1AG301100 Rh1BG263800 Rh1BG264000 Rh1BG264100 Rh1CG281800 Rh1CG282100 Rh1CG282200 Rh1DG294000 Rh1DG294100 Rh5BG200600
rosa_wichuraiana Rw1G005400 Rw1G011170 Rw1G026570 Rw1G026580 Rw1G026610 Rw1G026640 Rw1G026670 Rw1G026700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2023
AasI GACNNNNNNGTC 1 cut(s) 968
Acc16I TGCGCA 1 cut(s) 1716
Acc36I ACCTGC 1 cut(s) 2023
AccB1I GGYRCC 4 cut(s) 867, 924, 1308, 2096
AccB7I CCANNNNNTGG 3 cut(s) 1550, 2018, 2207
AccI GTMKAC 1 cut(s) 362
AccII CGCG 1 cut(s) 1395
AccIII TCCGGA 2 cut(s) 226, 1886
AciI CCGC 8 cut(s) 167, 170, 179, 182, 246, 358, 385, 1826
AclWI GGATC 7 cut(s) 8, 721, 1095, 1215, 1228, 1305, 1897
AcoI YGGCCR 2 cut(s) 607, 892
AcsI RAATTY 3 cut(s) 590, 1027, 1400
AcuI CTGAAG 3 cut(s) 620, 933, 1863
AcyI GRCGYC 1 cut(s) 562
AfaI GTAC 2 cut(s) 8, 1951
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 8 cut(s) 528, 626, 814, 1133, 1265, 1629, 1929, 2104
AjnI CCWGG 9 cut(s) 250, 553, 1257, 1378, 1498, 1543, 1657, 2159, 2191
AjuI GAANNNNNNNTTGG 2 cut(s) 1909, 1941
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 9 cut(s) 428, 795, 947, 967, 1102, 1129, 1559, 1643, 1795
AluI AGCT 9 cut(s) 428, 795, 947, 967, 1102, 1129, 1559, 1643, 1795
Alw21I GWGCWC 2 cut(s) 1645, 2113
Alw26I GTCTC 4 cut(s) 141, 700, 942, 1377
Alw44I GTGCAC 1 cut(s) 2109
AlwI GGATC 7 cut(s) 8, 721, 1095, 1215, 1228, 1305, 1897
AlwNI CAGNNNCTG 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 1514
Aor13HI TCCGGA 2 cut(s) 226, 1886
AoxI GGCC 8 cut(s) 160, 607, 753, 892, 1376, 1487, 2053, 2195
ApaI GGGCCC 2 cut(s) 164, 2057
ApaLI GTGCAC 1 cut(s) 2109
ApoI RAATTY 3 cut(s) 590, 1027, 1400
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 2 cut(s) 630, 780
AspLEI GCGC 2 cut(s) 882, 1717
AspS9I GGNCC 9 cut(s) 160, 161, 254, 1487, 1502, 1661, 2053, 2054, 2195
AsuC2I CCSGG 3 cut(s) 1515, 1516, 2139
AsuHPI GGTGA 6 cut(s) 74, 227, 296, 713, 1442, 1736
AsuII TTCGAA 1 cut(s) 830
AvaI CYCGRG 1 cut(s) 1514
AvaII GGWCC 3 cut(s) 254, 1502, 1661
BaeGI GKGCMC 3 cut(s) 164, 2057, 2113
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BalI TGGCCA 2 cut(s) 609, 894
BamHI GGATCC 1 cut(s) 1220
BanI GGYRCC 4 cut(s) 867, 924, 1308, 2096
BanII GRGCYC 3 cut(s) 164, 1645, 2057
BarI GAAGNNNNNNTAC 2 cut(s) 1861, 1893
Bbv12I GWGCWC 2 cut(s) 1645, 2113
BbvCI CCTCAGC 1 cut(s) 2181
BceAI ACGGC 2 cut(s) 184, 1549
BciT130I CCWGG 9 cut(s) 252, 555, 1259, 1380, 1500, 1545, 1659, 2161, 2193
BclI TGATCA 3 cut(s) 646, 1345, 1432
BcnI CCSGG 3 cut(s) 1515, 1516, 2139
BcoDI GTCTC 4 cut(s) 141, 700, 942, 1377
BfaI CTAG 2 cut(s) 345, 944
BfmI CTRYAG 1 cut(s) 1796
BfuAI ACCTGC 1 cut(s) 2023
BglII AGATCT 1 cut(s) 659
Bme18I GGWCC 3 cut(s) 254, 1502, 1661
BmeT110I CYCGRG 1 cut(s) 1514
BmgT120I GGNCC 9 cut(s) 160, 161, 254, 1487, 1502, 1661, 2053, 2054, 2195
BmsI GCATC 9 cut(s) 139, 180, 379, 973, 1156, 1186, 1598, 1837, 2028
BoxI GACNNNNGTC 1 cut(s) 1389
BpmI CTGGAG 3 cut(s) 691, 1482, 2040
Bpu10I CCTNAGC 1 cut(s) 2181
Bpu14I TTCGAA 1 cut(s) 830
BpuEI CTTGAG 2 cut(s) 811, 1404
BpuMI CCSGG 3 cut(s) 1515, 1516, 2139
BsaHI GRCGYC 1 cut(s) 562
BsaI GGTCTC 1 cut(s) 700
BsaWI WCCGGW 3 cut(s) 226, 1886, 2171
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bse1I ACTGG 4 cut(s) 895, 926, 1070, 1393
Bse3DI GCAATG 1 cut(s) 1348
BseAI TCCGGA 2 cut(s) 226, 1886
BseBI CCWGG 9 cut(s) 252, 555, 1259, 1380, 1500, 1545, 1659, 2161, 2193
BseMI GCAATG 1 cut(s) 1348
BseMII CTCAG 2 cut(s) 335, 2195
BseNI ACTGG 4 cut(s) 895, 926, 1070, 1393
BseRI GAGGAG 3 cut(s) 540, 1635, 1638
BseSI GKGCMC 3 cut(s) 164, 2057, 2113
BsgI GTGCAG 1 cut(s) 1387
Bsh1236I CGCG 1 cut(s) 1395
BshFI GGCC 8 cut(s) 162, 609, 755, 894, 1378, 1489, 2055, 2197
BshNI GGYRCC 4 cut(s) 867, 924, 1308, 2096
BsiHKAI GWGCWC 2 cut(s) 1645, 2113
BsiHKCI CYCGRG 1 cut(s) 1514
BsiSI CCGG 5 cut(s) 227, 1515, 1887, 2138, 2172
BsmAI GTCTC 4 cut(s) 141, 700, 942, 1377
BsnI GGCC 8 cut(s) 162, 609, 755, 894, 1378, 1489, 2055, 2197
Bso31I GGTCTC 1 cut(s) 700
BsoBI CYCGRG 1 cut(s) 1514
Bsp119I TTCGAA 1 cut(s) 830
Bsp120I GGGCCC 2 cut(s) 160, 2053
Bsp1286I GDGCHC 4 cut(s) 164, 1645, 2057, 2113
Bsp13I TCCGGA 2 cut(s) 226, 1886
Bsp19I CCATGG 1 cut(s) 895
Bsp68I TCGCGA 1 cut(s) 1395
BspACI CCGC 8 cut(s) 167, 170, 179, 182, 246, 358, 385, 1826
BspANI GGCC 8 cut(s) 162, 609, 755, 894, 1378, 1489, 2055, 2197
BspCNI CTCAG 2 cut(s) 334, 2194
BspEI TCCGGA 2 cut(s) 226, 1886
BspFNI CGCG 1 cut(s) 1395
BspHI TCATGA 2 cut(s) 643, 1186
BspMI ACCTGC 1 cut(s) 2023
BspPI GGATC 7 cut(s) 8, 721, 1095, 1215, 1228, 1305, 1897
BspQI GCTCTTC 1 cut(s) 870
BspT104I TTCGAA 1 cut(s) 830
BspT107I GGYRCC 4 cut(s) 867, 924, 1308, 2096
BspTNI GGTCTC 1 cut(s) 700
BsrDI GCAATG 1 cut(s) 1348
BsrI ACTGG 4 cut(s) 895, 926, 1070, 1393
BssNAI GTATAC 1 cut(s) 363
BssNI GRCGYC 1 cut(s) 562
BssT1I CCWWGG 2 cut(s) 895, 1738
Bst1107I GTATAC 1 cut(s) 363
Bst2UI CCWGG 9 cut(s) 252, 555, 1259, 1380, 1500, 1545, 1659, 2161, 2193
Bst4CI ACNGT 5 cut(s) 352, 406, 451, 867, 1289
Bst6I CTCTTC 3 cut(s) 870, 905, 1680
BstACI GRCGYC 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 830
BstC8I GCNNGC 2 cut(s) 1943, 2095
BstDEI CTNAG 4 cut(s) 321, 987, 1083, 2181
BstDSI CCRYGG 2 cut(s) 895, 1141
BstEII GGTNACC 1 cut(s) 1742
BstENI CCTNNNNNAGG 2 cut(s) 2019, 2190
BstFNI CGCG 1 cut(s) 1395
BstHHI GCGC 2 cut(s) 882, 1717
BstMAI GTCTC 4 cut(s) 141, 700, 942, 1377
BstNI CCWGG 9 cut(s) 252, 555, 1259, 1380, 1500, 1545, 1659, 2161, 2193
BstNSI RCATGY 3 cut(s) 442, 1945, 2050
BstPAI GACNNNNGTC 1 cut(s) 1389
BstPI GGTNACC 1 cut(s) 1742
BstSFI CTRYAG 1 cut(s) 1796
BstSLI GKGCMC 3 cut(s) 164, 2057, 2113
BstUI CGCG 1 cut(s) 1395
BstX2I RGATCY 4 cut(s) 659, 1220, 1297, 1889
BstYI RGATCY 4 cut(s) 659, 1220, 1297, 1889
BstZ17I GTATAC 1 cut(s) 363
BsuRI GGCC 8 cut(s) 162, 609, 755, 894, 1378, 1489, 2055, 2197
BtgI CCRYGG 2 cut(s) 895, 1141
BtsI GCAGTG 1 cut(s) 1339
BtsIMutI CAGTG 6 cut(s) 348, 1210, 1294, 1339, 1386, 1887
BtuMI TCGCGA 1 cut(s) 1395
BveI ACCTGC 1 cut(s) 2023
Cac8I GCNNGC 2 cut(s) 1943, 2095
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 2 cut(s) 643, 1186
CfoI GCGC 2 cut(s) 882, 1717
Cfr13I GGNCC 9 cut(s) 160, 161, 254, 1487, 1502, 1661, 2053, 2054, 2195
Cfr9I CCCGGG 1 cut(s) 1514
CseI GACGC 3 cut(s) 182, 551, 1595
CsiI ACCWGGT 1 cut(s) 1657
Csp6I GTAC 2 cut(s) 7, 1950
CviQI GTAC 2 cut(s) 7, 1950
DdeI CTNAG 4 cut(s) 321, 987, 1083, 2181
DrdI GACNNNNNNGTC 1 cut(s) 968
DseDI GACNNNNNNGTC 1 cut(s) 968
EaeI YGGCCR 2 cut(s) 607, 892
Eam1104I CTCTTC 3 cut(s) 870, 905, 1680
EarI CTCTTC 3 cut(s) 870, 905, 1680
EciI GGCGGA 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 1643
Eco130I CCWWGG 2 cut(s) 895, 1738
Eco24I GRGCYC 3 cut(s) 164, 1645, 2057
Eco31I GGTCTC 1 cut(s) 700
Eco47I GGWCC 3 cut(s) 254, 1502, 1661
Eco53kI GAGCTC 1 cut(s) 1643
Eco57I CTGAAG 3 cut(s) 620, 933, 1863
Eco88I CYCGRG 1 cut(s) 1514
Eco91I GGTNACC 1 cut(s) 1742
EcoICRI GAGCTC 1 cut(s) 1643
EcoNI CCTNNNNNAGG 2 cut(s) 2019, 2190
EcoO109I RGGNCCY 2 cut(s) 160, 2053
EcoO65I GGTNACC 1 cut(s) 1742
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 9 cut(s) 250, 553, 1257, 1378, 1498, 1543, 1657, 2159, 2191
EcoT14I CCWWGG 2 cut(s) 895, 1738
EcoT38I GRGCYC 3 cut(s) 164, 1645, 2057
ErhI CCWWGG 2 cut(s) 895, 1738
FalI AAGNNNNNCTT 4 cut(s) 794, 826, 951, 983
FauI CCCGC 1 cut(s) 378
FbaI TGATCA 3 cut(s) 646, 1345, 1432
FblI GTMKAC 1 cut(s) 362
FriOI GRGCYC 3 cut(s) 164, 1645, 2057
FspBI CTAG 2 cut(s) 345, 944
FspI TGCGCA 1 cut(s) 1716
GlaI GCGC 2 cut(s) 881, 1716
GsuI CTGGAG 3 cut(s) 691, 1482, 2040
HaeIII GGCC 8 cut(s) 162, 609, 755, 894, 1378, 1489, 2055, 2197
HapII CCGG 5 cut(s) 227, 1515, 1887, 2138, 2172
HgaI GACGC 3 cut(s) 182, 551, 1595
HhaI GCGC 2 cut(s) 882, 1717
Hin1I GRCGYC 1 cut(s) 562
Hin6I GCGC 2 cut(s) 880, 1715
HinP1I GCGC 2 cut(s) 880, 1715
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 9 cut(s) 319, 370, 547, 631, 673, 776, 1133, 1417, 1957
HpaII CCGG 5 cut(s) 227, 1515, 1887, 2138, 2172
HphI GGTGA 6 cut(s) 74, 227, 296, 713, 1442, 1736
Hpy166II GTNNAC 6 cut(s) 363, 546, 919, 1664, 1844, 2111
Hpy188I TCNGA 7 cut(s) 324, 463, 659, 1152, 1216, 1416, 1962
Hpy188III TCNNGA 8 cut(s) 227, 644, 670, 785, 1187, 1394, 1887, 2118
Hpy8I GTNNAC 6 cut(s) 363, 546, 919, 1664, 1844, 2111
HpyAV CCTTC 8 cut(s) 321, 745, 1117, 1147, 1526, 1680, 1914, 2110
HpyCH4III ACNGT 5 cut(s) 352, 406, 451, 867, 1289
HpyCH4IV ACGT 1 cut(s) 1840
HpyF3I CTNAG 4 cut(s) 321, 987, 1083, 2181
HpySE526I ACGT 1 cut(s) 1840
Hsp92I GRCGYC 1 cut(s) 562
HspAI GCGC 2 cut(s) 880, 1715
Kpn2I TCCGGA 2 cut(s) 226, 1886
Ksp22I TGATCA 3 cut(s) 646, 1345, 1432
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 4 cut(s) 229, 1126, 1640, 1648
LweI GCATC 9 cut(s) 139, 180, 379, 973, 1156, 1186, 1598, 1837, 2028
MabI ACCWGGT 1 cut(s) 1657
MaeI CTAG 2 cut(s) 345, 944
MaeII ACGT 1 cut(s) 1840
MaeIII GTNAC 3 cut(s) 1019, 1159, 1742
MboII GAAGA 7 cut(s) 201, 626, 887, 922, 1202, 1667, 1869
MfeI CAATTG 2 cut(s) 432, 1973
MflI RGATCY 4 cut(s) 659, 1220, 1297, 1889
MhlI GDGCHC 4 cut(s) 164, 1645, 2057, 2113
MlsI TGGCCA 2 cut(s) 609, 894
MluNI TGGCCA 2 cut(s) 609, 894
MlyI GAGTC 5 cut(s) 313, 364, 541, 682, 1411
MmeI TCCRAC 2 cut(s) 1531, 1940
Mox20I TGGCCA 2 cut(s) 609, 894
MroI TCCGGA 2 cut(s) 226, 1886
MroXI GAANNNNTTC 2 cut(s) 630, 780
MscI TGGCCA 2 cut(s) 609, 894
MseI TTAA 5 cut(s) 531, 618, 846, 1044, 1404
MslI CAYNNNNRTG 3 cut(s) 285, 353, 1710
Msp20I TGGCCA 2 cut(s) 609, 894
MspA1I CMGCKG 2 cut(s) 385, 428
MspI CCGG 5 cut(s) 227, 1515, 1887, 2138, 2172
MunI CAATTG 2 cut(s) 432, 1973
MvaI CCWGG 9 cut(s) 252, 555, 1259, 1380, 1500, 1545, 1659, 2161, 2193
MvnI CGCG 1 cut(s) 1395
NciI CCSGG 3 cut(s) 1515, 1516, 2139
NcoI CCATGG 1 cut(s) 895
NmuCI GTSAC 3 cut(s) 1019, 1159, 1742
NruI TCGCGA 1 cut(s) 1395
NsbI TGCGCA 1 cut(s) 1716
NspI RCATGY 3 cut(s) 442, 1945, 2050
NspV TTCGAA 1 cut(s) 830
OliI CACNNNNGTG 1 cut(s) 353
PaeI GCATGC 1 cut(s) 1945
PagI TCATGA 2 cut(s) 643, 1186
PaqCI CACCTGC 1 cut(s) 2023
PasI CCCWGGG 1 cut(s) 2160
PciI ACATGT 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 870
PdmI GAANNNNTTC 2 cut(s) 630, 780
PfeI GAWTC 4 cut(s) 631, 776, 1133, 1957
PflMI CCANNNNNTGG 3 cut(s) 1550, 2018, 2207
PfoI TCCNGGA 1 cut(s) 1498
PleI GAGTC 5 cut(s) 313, 364, 541, 681, 1411
PpsI GAGTC 5 cut(s) 313, 364, 541, 681, 1411
PscI ACATGT 1 cut(s) 438
PshAI GACNNNNGTC 1 cut(s) 1389
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 1645
Psp6I CCWGG 9 cut(s) 250, 553, 1257, 1378, 1498, 1543, 1657, 2159, 2191
PspEI GGTNACC 1 cut(s) 1742
PspGI CCWGG 9 cut(s) 250, 553, 1257, 1378, 1498, 1543, 1657, 2159, 2191
PspOMI GGGCCC 2 cut(s) 160, 2053
PspPI GGNCC 9 cut(s) 160, 161, 254, 1487, 1502, 1661, 2053, 2054, 2195
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 4 cut(s) 659, 1220, 1297, 1889
PvuII CAGCTG 1 cut(s) 428
RruI TCGCGA 1 cut(s) 1395
RsaI GTAC 2 cut(s) 8, 1951
RsaNI GTAC 2 cut(s) 7, 1950
RseI CAYNNNNRTG 3 cut(s) 285, 353, 1710
SacI GAGCTC 1 cut(s) 1645
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 5 cut(s) 531, 618, 846, 1044, 1404
Sau96I GGNCC 9 cut(s) 160, 161, 254, 1487, 1502, 1661, 2053, 2054, 2195
SchI GAGTC 5 cut(s) 313, 364, 541, 682, 1411
SduI GDGCHC 4 cut(s) 164, 1645, 2057, 2113
SexAI ACCWGGT 1 cut(s) 1657
SfaNI GCATC 9 cut(s) 139, 180, 379, 973, 1156, 1186, 1598, 1837, 2028
SfcI CTRYAG 1 cut(s) 1796
SfuI TTCGAA 1 cut(s) 830
SinI GGWCC 3 cut(s) 254, 1502, 1661
SmaI CCCGGG 1 cut(s) 1516
SmiMI CAYNNNNRTG 3 cut(s) 285, 353, 1710
SmlI CTYRAG 2 cut(s) 790, 1419
SmoI CTYRAG 2 cut(s) 790, 1419
SphI GCATGC 1 cut(s) 1945
SsiI CCGC 8 cut(s) 167, 170, 179, 182, 246, 358, 385, 1826
SspI AATATT 1 cut(s) 1196
SspMI CTAG 2 cut(s) 345, 944
SstI GAGCTC 1 cut(s) 1645
StyI CCWWGG 2 cut(s) 895, 1738
TaaI ACNGT 5 cut(s) 352, 406, 451, 867, 1289
TaiI ACGT 1 cut(s) 1843
TaqI TCGA 3 cut(s) 373, 830, 1525
TauI GCSGC 4 cut(s) 172, 181, 184, 1828
TfiI GAWTC 4 cut(s) 631, 776, 1133, 1957
Tru1I TTAA 5 cut(s) 531, 618, 846, 1044, 1404
Tru9I TTAA 5 cut(s) 531, 618, 846, 1044, 1404
TscAI CASTG 6 cut(s) 355, 1210, 1294, 1339, 1393, 1887
TseFI GTSAC 3 cut(s) 1019, 1159, 1742
Tsp45I GTSAC 3 cut(s) 1019, 1159, 1742
TspGWI ACGGA 2 cut(s) 509, 1130
TspMI CCCGGG 1 cut(s) 1514
TspRI CASTG 6 cut(s) 355, 1210, 1294, 1339, 1393, 1887
Van91I CCANNNNNTGG 3 cut(s) 1550, 2018, 2207
VneI GTGCAC 1 cut(s) 2109
VpaK11BI GGWCC 3 cut(s) 254, 1502, 1661
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 2 cut(s) 2019, 2190
XapI RAATTY 3 cut(s) 590, 1027, 1400
XceI RCATGY 3 cut(s) 442, 1945, 2050
XcmI CCANNNNNNNNNTGG 1 cut(s) 1238
XmaI CCCGGG 1 cut(s) 1514
XmiI GTMKAC 1 cut(s) 362
XmnI GAANNNNTTC 2 cut(s) 630, 780
XspI CTAG 2 cut(s) 345, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.