Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 689 |
| AcoI | YGGCCR | 1 cut(s) | 843 |
| AcsI | RAATTY | 2 cut(s) | 134, 808 |
| AcuI | CTGAAG | 2 cut(s) | 93, 233 |
| AfaI | GTAC | 3 cut(s) | 574, 664, 802 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 422, 640, 658 |
| AgsI | TTSAA | 5 cut(s) | 20, 140, 326, 564, 853 |
| AhlI | ACTAGT | 1 cut(s) | 163 |
| AjnI | CCWGG | 1 cut(s) | 315 |
| AluBI | AGCT | 7 cut(s) | 113, 143, 239, 388, 458, 488, 785 |
| AluI | AGCT | 7 cut(s) | 113, 143, 239, 388, 458, 488, 785 |
| Alw21I | GWGCWC | 1 cut(s) | 171 |
| Alw26I | GTCTC | 1 cut(s) | 635 |
| AlwI | GGATC | 1 cut(s) | 689 |
| AoxI | GGCC | 3 cut(s) | 775, 824, 843 |
| ApeKI | GCWGC | 1 cut(s) | 582 |
| ApoI | RAATTY | 2 cut(s) | 134, 808 |
| AspS9I | GGNCC | 1 cut(s) | 775 |
| AsuHPI | GGTGA | 1 cut(s) | 45 |
| AxyI | CCTNAGG | 1 cut(s) | 828 |
| BalI | TGGCCA | 1 cut(s) | 845 |
| BbsI | GAAGAC | 1 cut(s) | 210 |
| Bbv12I | GWGCWC | 1 cut(s) | 171 |
| BbvCI | CCTCAGC | 1 cut(s) | 459 |
| BbvI | GCAGC | 1 cut(s) | 569 |
| BccI | CCATC | 1 cut(s) | 642 |
| BciT130I | CCWGG | 1 cut(s) | 317 |
| BciVI | GTATCC | 2 cut(s) | 143, 877 |
| BclI | TGATCA | 1 cut(s) | 295 |
| BcoDI | GTCTC | 1 cut(s) | 635 |
| BcuI | ACTAGT | 1 cut(s) | 163 |
| BfaI | CTAG | 1 cut(s) | 164 |
| BfmI | CTRYAG | 1 cut(s) | 935 |
| BfuI | GTATCC | 2 cut(s) | 143, 877 |
| BisI | GCNGC | 1 cut(s) | 583 |
| BlsI | GCNGC | 1 cut(s) | 584 |
| Bme1390I | CCNGG | 1 cut(s) | 317 |
| BmgT120I | GGNCC | 1 cut(s) | 775 |
| BmiI | GGNNCC | 1 cut(s) | 715 |
| BmrFI | CCNGG | 1 cut(s) | 317 |
| BmsI | GCATC | 2 cut(s) | 319, 511 |
| BpiI | GAAGAC | 1 cut(s) | 210 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 491, 523 |
| BpmI | CTGGAG | 2 cut(s) | 165, 627 |
| Bpu10I | CCTNAGC | 1 cut(s) | 459 |
| BsaJI | CCNNGG | 3 cut(s) | 639, 652, 916 |
| BsaWI | WCCGGW | 1 cut(s) | 81 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 447, 477 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 422, 640, 658 |
| Bse1I | ACTGG | 1 cut(s) | 259 |
| Bse21I | CCTNAGG | 1 cut(s) | 828 |
| Bse3DI | GCAATG | 1 cut(s) | 871 |
| BseBI | CCWGG | 1 cut(s) | 317 |
| BseDI | CCNNGG | 3 cut(s) | 639, 652, 916 |
| BseGI | GGATG | 2 cut(s) | 526, 634 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 422, 640, 658 |
| BseMI | GCAATG | 1 cut(s) | 871 |
| BseMII | CTCAG | 1 cut(s) | 450 |
| BseNI | ACTGG | 1 cut(s) | 259 |
| BseXI | GCAGC | 1 cut(s) | 569 |
| BsgI | GTGCAG | 1 cut(s) | 915 |
| Bsh1285I | CGRYCG | 1 cut(s) | 86 |
| BshFI | GGCC | 3 cut(s) | 777, 826, 845 |
| BsiEI | CGRYCG | 1 cut(s) | 86 |
| BsiHKAI | GWGCWC | 1 cut(s) | 171 |
| BsiSI | CCGG | 1 cut(s) | 82 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 422, 640, 658 |
| BsmAI | GTCTC | 1 cut(s) | 635 |
| BsmI | GAATGC | 1 cut(s) | 367 |
| BsnI | GGCC | 3 cut(s) | 777, 826, 845 |
| Bsp1286I | GDGCHC | 1 cut(s) | 171 |
| Bsp143I | GATC | 4 cut(s) | 295, 513, 694, 751 |
| Bsp19I | CCATGG | 1 cut(s) | 916 |
| BspANI | GGCC | 3 cut(s) | 777, 826, 845 |
| BspCNI | CTCAG | 1 cut(s) | 451 |
| BspLI | GGNNCC | 1 cut(s) | 715 |
| BspPI | GGATC | 1 cut(s) | 689 |
| BspQI | GCTCTTC | 1 cut(s) | 478 |
| BsrDI | GCAATG | 1 cut(s) | 871 |
| BsrI | ACTGG | 1 cut(s) | 259 |
| BssECI | CCNNGG | 3 cut(s) | 639, 652, 916 |
| BssMI | GATC | 4 cut(s) | 295, 513, 694, 751 |
| BssT1I | CCWWGG | 3 cut(s) | 639, 652, 916 |
| Bst2UI | CCWGG | 1 cut(s) | 317 |
| Bst4CI | ACNGT | 1 cut(s) | 936 |
| Bst6I | CTCTTC | 1 cut(s) | 478 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 908 |
| BstC8I | GCNNGC | 5 cut(s) | 490, 536, 824, 843, 862 |
| BstDEI | CTNAG | 2 cut(s) | 459, 828 |
| BstDSI | CCRYGG | 1 cut(s) | 916 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 656 |
| BstF5I | GGATG | 2 cut(s) | 526, 634 |
| BstKTI | GATC | 4 cut(s) | 298, 516, 697, 754 |
| BstMAI | GTCTC | 1 cut(s) | 635 |
| BstMBI | GATC | 4 cut(s) | 295, 513, 694, 751 |
| BstMCI | CGRYCG | 1 cut(s) | 86 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 908 |
| BstNI | CCWGG | 1 cut(s) | 317 |
| BstNSI | RCATGY | 2 cut(s) | 313, 542 |
| BstSCI | CCNGG | 1 cut(s) | 315 |
| BstSFI | CTRYAG | 1 cut(s) | 935 |
| BstV1I | GCAGC | 1 cut(s) | 569 |
| BstV2I | GAAGAC | 1 cut(s) | 210 |
| Bsu36I | CCTNAGG | 1 cut(s) | 828 |
| BsuI | GTATCC | 2 cut(s) | 143, 877 |
| BsuRI | GGCC | 3 cut(s) | 777, 826, 845 |
| BtgI | CCRYGG | 1 cut(s) | 916 |
| BtsCI | GGATG | 2 cut(s) | 526, 634 |
| BtsI | GCAGTG | 1 cut(s) | 600 |
| BtsIMutI | CAGTG | 3 cut(s) | 99, 600, 932 |
| Cac8I | GCNNGC | 5 cut(s) | 490, 536, 824, 843, 862 |
| Cfr13I | GGNCC | 1 cut(s) | 775 |
| Csp6I | GTAC | 3 cut(s) | 573, 663, 801 |
| CviAII | CATG | 4 cut(s) | 310, 356, 539, 917 |
| CviQI | GTAC | 3 cut(s) | 573, 663, 801 |
| DdeI | CTNAG | 2 cut(s) | 459, 828 |
| DpnI | GATC | 4 cut(s) | 297, 515, 696, 753 |
| DpnII | GATC | 4 cut(s) | 295, 513, 694, 751 |
| EaeI | YGGCCR | 1 cut(s) | 843 |
| Eam1104I | CTCTTC | 1 cut(s) | 478 |
| EarI | CTCTTC | 1 cut(s) | 478 |
| Eco130I | CCWWGG | 3 cut(s) | 639, 652, 916 |
| Eco57I | CTGAAG | 2 cut(s) | 93, 233 |
| Eco81I | CCTNAGG | 1 cut(s) | 828 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 656 |
| EcoRII | CCWGG | 1 cut(s) | 315 |
| EcoT14I | CCWWGG | 3 cut(s) | 639, 652, 916 |
| EcoT22I | ATGCAT | 1 cut(s) | 369 |
| ErhI | CCWWGG | 3 cut(s) | 639, 652, 916 |
| FaeI | CATG | 4 cut(s) | 313, 359, 542, 920 |
| FatI | CATG | 4 cut(s) | 309, 355, 538, 916 |
| FbaI | TGATCA | 1 cut(s) | 295 |
| Fnu4HI | GCNGC | 1 cut(s) | 583 |
| FokI | GGATG | 2 cut(s) | 533, 621 |
| Fsp4HI | GCNGC | 1 cut(s) | 583 |
| FspBI | CTAG | 1 cut(s) | 164 |
| GluI | GCNGC | 1 cut(s) | 583 |
| GsuI | CTGGAG | 2 cut(s) | 165, 627 |
| HaeIII | GGCC | 3 cut(s) | 777, 826, 845 |
| HapII | CCGG | 1 cut(s) | 82 |
| Hin1II | CATG | 4 cut(s) | 313, 359, 542, 920 |
| HindIII | AAGCTT | 1 cut(s) | 386 |
| HinfI | GANTC | 5 cut(s) | 50, 125, 242, 500, 853 |
| HpaII | CCGG | 1 cut(s) | 82 |
| HphI | GGTGA | 1 cut(s) | 45 |
| Hpy166II | GTNNAC | 1 cut(s) | 220 |
| Hpy188I | TCNGA | 5 cut(s) | 49, 252, 300, 592, 814 |
| Hpy188III | TCNNGA | 5 cut(s) | 129, 326, 375, 508, 698 |
| Hpy8I | GTNNAC | 1 cut(s) | 220 |
| HpyAV | CCTTC | 3 cut(s) | 426, 541, 874 |
| HpyCH4III | ACNGT | 1 cut(s) | 936 |
| HpyCH4IV | ACGT | 1 cut(s) | 789 |
| HpyCH4V | TGCA | 6 cut(s) | 367, 534, 585, 728, 891, 932 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 908 |
| HpyF3I | CTNAG | 2 cut(s) | 459, 828 |
| HpySE526I | ACGT | 1 cut(s) | 789 |
| Hsp92II | CATG | 4 cut(s) | 313, 359, 542, 920 |
| Ksp22I | TGATCA | 1 cut(s) | 295 |
| Kzo9I | GATC | 4 cut(s) | 295, 513, 694, 751 |
| LguI | GCTCTTC | 1 cut(s) | 478 |
| LmnI | GCTCC | 2 cut(s) | 236, 455 |
| Lsp1109I | GCAGC | 1 cut(s) | 569 |
| LweI | GCATC | 2 cut(s) | 319, 511 |
| MaeI | CTAG | 1 cut(s) | 164 |
| MaeII | ACGT | 1 cut(s) | 789 |
| MaeIII | GTNAC | 1 cut(s) | 925 |
| MalI | GATC | 4 cut(s) | 297, 515, 696, 753 |
| MboI | GATC | 4 cut(s) | 295, 513, 694, 751 |
| MboII | GAAGA | 7 cut(s) | 202, 215, 314, 363, 495, 523, 634 |
| MhlI | GDGCHC | 1 cut(s) | 171 |
| MlsI | TGGCCA | 1 cut(s) | 845 |
| MluCI | AATT | 5 cut(s) | 134, 442, 686, 808, 921 |
| MluNI | TGGCCA | 1 cut(s) | 845 |
| MlyI | GAGTC | 2 cut(s) | 134, 509 |
| MmeI | TCCRAC | 2 cut(s) | 83, 214 |
| MnlI | CCTC | 8 cut(s) | 66, 140, 454, 602, 766, 814, 892, 898 |
| Mox20I | TGGCCA | 1 cut(s) | 845 |
| Mph1103I | ATGCAT | 1 cut(s) | 369 |
| MscI | TGGCCA | 1 cut(s) | 845 |
| MseI | TTAA | 1 cut(s) | 528 |
| Msp20I | TGGCCA | 1 cut(s) | 845 |
| MspA1I | CMGCKG | 1 cut(s) | 113 |
| MspI | CCGG | 1 cut(s) | 82 |
| MspR9I | CCNGG | 1 cut(s) | 317 |
| Mva1269I | GAATGC | 1 cut(s) | 367 |
| MvaI | CCWGG | 1 cut(s) | 317 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 908 |
| NcoI | CCATGG | 1 cut(s) | 916 |
| NdeII | GATC | 4 cut(s) | 295, 513, 694, 751 |
| NlaIII | CATG | 4 cut(s) | 313, 359, 542, 920 |
| NlaIV | GGNNCC | 1 cut(s) | 715 |
| NsiI | ATGCAT | 1 cut(s) | 369 |
| NspI | RCATGY | 2 cut(s) | 313, 542 |
| PciSI | GCTCTTC | 1 cut(s) | 478 |
| PctI | GAATGC | 1 cut(s) | 367 |
| PfeI | GAWTC | 3 cut(s) | 50, 242, 853 |
| PkrI | GCNGC | 1 cut(s) | 584 |
| PleI | GAGTC | 2 cut(s) | 133, 508 |
| PpsI | GAGTC | 2 cut(s) | 133, 508 |
| Psp6I | CCWGG | 1 cut(s) | 315 |
| PspGI | CCWGG | 1 cut(s) | 315 |
| PspN4I | GGNNCC | 1 cut(s) | 715 |
| PspPI | GGNCC | 1 cut(s) | 775 |
| PvuII | CAGCTG | 1 cut(s) | 113 |
| RsaI | GTAC | 3 cut(s) | 574, 664, 802 |
| RsaNI | GTAC | 3 cut(s) | 573, 663, 801 |
| SapI | GCTCTTC | 1 cut(s) | 478 |
| SaqAI | TTAA | 1 cut(s) | 528 |
| SatI | GCNGC | 1 cut(s) | 583 |
| Sau3AI | GATC | 4 cut(s) | 295, 513, 694, 751 |
| Sau96I | GGNCC | 1 cut(s) | 775 |
| SchI | GAGTC | 2 cut(s) | 134, 509 |
| ScrFI | CCNGG | 1 cut(s) | 317 |
| SduI | GDGCHC | 1 cut(s) | 171 |
| SfaNI | GCATC | 2 cut(s) | 319, 511 |
| SfcI | CTRYAG | 1 cut(s) | 935 |
| SpeI | ACTAGT | 1 cut(s) | 163 |
| Sse9I | AATT | 5 cut(s) | 134, 442, 686, 808, 921 |
| SspMI | CTAG | 1 cut(s) | 164 |
| StyD4I | CCNGG | 1 cut(s) | 315 |
| StyI | CCWWGG | 3 cut(s) | 639, 652, 916 |
| TaaI | ACNGT | 1 cut(s) | 936 |
| TaiI | ACGT | 1 cut(s) | 792 |
| TaqI | TCGA | 3 cut(s) | 35, 509, 754 |
| TasI | AATT | 5 cut(s) | 134, 442, 686, 808, 921 |
| TfiI | GAWTC | 3 cut(s) | 50, 242, 853 |
| Tru1I | TTAA | 1 cut(s) | 528 |
| Tru9I | TTAA | 1 cut(s) | 528 |
| TscAI | CASTG | 3 cut(s) | 99, 607, 939 |
| TseI | GCWGC | 1 cut(s) | 582 |
| TspDTI | ATGAA | 1 cut(s) | 276 |
| TspGWI | ACGGA | 1 cut(s) | 628 |
| TspRI | CASTG | 3 cut(s) | 99, 607, 939 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 656 |
| XapI | RAATTY | 2 cut(s) | 134, 808 |
| XceI | RCATGY | 2 cut(s) | 313, 542 |
| XspI | CTAG | 1 cut(s) | 164 |
| Zsp2I | ATGCAT | 1 cut(s) | 369 |