Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 368, 859 |
| AccI | GTMKAC | 2 cut(s) | 438, 873 |
| AciI | CCGC | 1 cut(s) | 56 |
| AclWI | GGATC | 1 cut(s) | 762 |
| AcsI | RAATTY | 4 cut(s) | 560, 759, 842, 933 |
| AfaI | GTAC | 4 cut(s) | 110, 155, 823, 994 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 16, 226 |
| AflIII | ACRYGT | 2 cut(s) | 446, 541 |
| AgsI | TTSAA | 6 cut(s) | 104, 143, 329, 404, 851, 889 |
| AhlI | ACTAGT | 1 cut(s) | 956 |
| AjnI | CCWGG | 1 cut(s) | 712 |
| AleI | CACNNNNGTG | 1 cut(s) | 813 |
| AluBI | AGCT | 2 cut(s) | 146, 631 |
| AluI | AGCT | 2 cut(s) | 146, 631 |
| AlwI | GGATC | 1 cut(s) | 762 |
| ApeKI | GCWGC | 1 cut(s) | 578 |
| ApoI | RAATTY | 4 cut(s) | 560, 759, 842, 933 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 244, 800 |
| AsuHPI | GGTGA | 4 cut(s) | 45, 167, 758, 931 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 435, 468 |
| BbvCI | CCTCAGC | 1 cut(s) | 986 |
| BbvI | GCAGC | 1 cut(s) | 565 |
| BccI | CCATC | 1 cut(s) | 20 |
| BceAI | ACGGC | 1 cut(s) | 822 |
| BciT130I | CCWGG | 1 cut(s) | 714 |
| BcuI | ACTAGT | 1 cut(s) | 956 |
| BfaI | CTAG | 6 cut(s) | 251, 434, 509, 693, 786, 957 |
| BfmI | CTRYAG | 1 cut(s) | 72 |
| BfuAI | ACCTGC | 2 cut(s) | 368, 859 |
| BisI | GCNGC | 1 cut(s) | 579 |
| BlsI | GCNGC | 1 cut(s) | 580 |
| Bme1390I | CCNGG | 1 cut(s) | 714 |
| BmiI | GGNNCC | 2 cut(s) | 460, 803 |
| BmrFI | CCNGG | 1 cut(s) | 714 |
| BmsI | GCATC | 5 cut(s) | 322, 565, 781, 886, 990 |
| BpmI | CTGGAG | 1 cut(s) | 168 |
| Bpu10I | CCTNAGC | 1 cut(s) | 986 |
| BsaBI | GATNNNNATC | 1 cut(s) | 127 |
| BsaJI | CCNNGG | 4 cut(s) | 462, 639, 712, 928 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 200, 230, 450, 480 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 16, 226 |
| Bse1I | ACTGG | 2 cut(s) | 171, 803 |
| Bse3DI | GCAATG | 1 cut(s) | 372 |
| Bse8I | GATNNNNATC | 1 cut(s) | 127 |
| BseBI | CCWGG | 1 cut(s) | 714 |
| BseDI | CCNNGG | 4 cut(s) | 462, 639, 712, 928 |
| BseGI | GGATG | 4 cut(s) | 522, 580, 650, 778 |
| BseJI | GATNNNNATC | 1 cut(s) | 127 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 16, 226 |
| BseMI | GCAATG | 1 cut(s) | 372 |
| BseMII | CTCAG | 2 cut(s) | 126, 977 |
| BseNI | ACTGG | 2 cut(s) | 171, 803 |
| BseXI | GCAGC | 1 cut(s) | 565 |
| BseYI | CCCAGC | 1 cut(s) | 605 |
| BsgI | GTGCAG | 1 cut(s) | 396 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 16, 226 |
| BsmI | GAATGC | 1 cut(s) | 747 |
| Bsp143I | GATC | 3 cut(s) | 38, 298, 754 |
| Bsp19I | CCATGG | 1 cut(s) | 928 |
| BspACI | CCGC | 1 cut(s) | 56 |
| BspCNI | CTCAG | 2 cut(s) | 125, 978 |
| BspLI | GGNNCC | 2 cut(s) | 460, 803 |
| BspMI | ACCTGC | 2 cut(s) | 368, 859 |
| BspPI | GGATC | 1 cut(s) | 762 |
| BsrDI | GCAATG | 1 cut(s) | 372 |
| BsrI | ACTGG | 2 cut(s) | 171, 803 |
| BssECI | CCNNGG | 4 cut(s) | 462, 639, 712, 928 |
| BssMI | GATC | 3 cut(s) | 38, 298, 754 |
| BssT1I | CCWWGG | 2 cut(s) | 639, 928 |
| Bst2UI | CCWGG | 1 cut(s) | 714 |
| Bst4CI | ACNGT | 5 cut(s) | 84, 288, 597, 821, 925 |
| BstC8I | GCNNGC | 1 cut(s) | 901 |
| BstDEI | CTNAG | 3 cut(s) | 112, 792, 986 |
| BstDSI | CCRYGG | 1 cut(s) | 928 |
| BstF5I | GGATG | 4 cut(s) | 522, 580, 650, 778 |
| BstKTI | GATC | 3 cut(s) | 41, 301, 757 |
| BstMBI | GATC | 3 cut(s) | 38, 298, 754 |
| BstNI | CCWGG | 1 cut(s) | 714 |
| BstNSI | RCATGY | 2 cut(s) | 450, 545 |
| BstSCI | CCNGG | 1 cut(s) | 712 |
| BstSFI | CTRYAG | 1 cut(s) | 72 |
| BstV1I | GCAGC | 1 cut(s) | 565 |
| BtgI | CCRYGG | 1 cut(s) | 928 |
| BtsCI | GGATG | 4 cut(s) | 522, 580, 650, 778 |
| BtsI | GCAGTG | 1 cut(s) | 600 |
| BtsIMutI | CAGTG | 1 cut(s) | 600 |
| BveI | ACCTGC | 2 cut(s) | 368, 859 |
| Cac8I | GCNNGC | 1 cut(s) | 901 |
| Csp6I | GTAC | 4 cut(s) | 109, 154, 822, 993 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 628, 663 |
| CviAII | CATG | 5 cut(s) | 359, 447, 542, 688, 929 |
| CviJI | RGCY | 7 cut(s) | 146, 461, 527, 612, 631, 692, 717 |
| CviKI_1 | RGCY | 7 cut(s) | 146, 461, 527, 612, 631, 692, 717 |
| CviQI | GTAC | 4 cut(s) | 109, 154, 822, 993 |
| DdeI | CTNAG | 3 cut(s) | 112, 792, 986 |
| DpnI | GATC | 3 cut(s) | 40, 300, 756 |
| DpnII | GATC | 3 cut(s) | 38, 298, 754 |
| DraI | TTTAAA | 1 cut(s) | 979 |
| Eco130I | CCWWGG | 2 cut(s) | 639, 928 |
| EcoRII | CCWGG | 1 cut(s) | 712 |
| EcoT14I | CCWWGG | 2 cut(s) | 639, 928 |
| ErhI | CCWWGG | 2 cut(s) | 639, 928 |
| FaeI | CATG | 5 cut(s) | 362, 450, 545, 691, 932 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 338, 370 |
| FatI | CATG | 5 cut(s) | 358, 446, 541, 687, 928 |
| FauNDI | CATATG | 1 cut(s) | 583 |
| FblI | GTMKAC | 2 cut(s) | 438, 873 |
| Fnu4HI | GCNGC | 1 cut(s) | 579 |
| FokI | GGATG | 4 cut(s) | 529, 587, 657, 765 |
| Fsp4HI | GCNGC | 1 cut(s) | 579 |
| FspBI | CTAG | 6 cut(s) | 251, 434, 509, 693, 786, 957 |
| GluI | GCNGC | 1 cut(s) | 579 |
| GsaI | CCCAGC | 1 cut(s) | 609 |
| GsuI | CTGGAG | 1 cut(s) | 168 |
| Hin1II | CATG | 5 cut(s) | 362, 450, 545, 691, 932 |
| HincII | GTYRAC | 1 cut(s) | 439 |
| HindII | GTYRAC | 1 cut(s) | 439 |
| HindIII | AAGCTT | 1 cut(s) | 629 |
| HinfI | GANTC | 4 cut(s) | 50, 128, 245, 587 |
| HphI | GGTGA | 4 cut(s) | 45, 167, 758, 931 |
| Hpy166II | GTNNAC | 3 cut(s) | 439, 600, 874 |
| Hpy188I | TCNGA | 7 cut(s) | 49, 115, 284, 303, 592, 699, 721 |
| Hpy188III | TCNNGA | 2 cut(s) | 401, 752 |
| Hpy8I | GTNNAC | 3 cut(s) | 439, 600, 874 |
| Hpy99I | CGWCG | 1 cut(s) | 443 |
| HpyAV | CCTTC | 5 cut(s) | 127, 514, 544, 647, 792 |
| HpyCH4III | ACNGT | 5 cut(s) | 84, 288, 597, 821, 925 |
| HpyCH4IV | ACGT | 1 cut(s) | 107 |
| HpyCH4V | TGCA | 5 cut(s) | 377, 537, 581, 685, 903 |
| HpyF3I | CTNAG | 3 cut(s) | 112, 792, 986 |
| HpySE526I | ACGT | 1 cut(s) | 107 |
| Hsp92II | CATG | 5 cut(s) | 362, 450, 545, 691, 932 |
| Kzo9I | GATC | 3 cut(s) | 38, 298, 754 |
| LmnI | GCTCC | 1 cut(s) | 458 |
| Lsp1109I | GCAGC | 1 cut(s) | 565 |
| LweI | GCATC | 5 cut(s) | 322, 565, 781, 886, 990 |
| MaeI | CTAG | 6 cut(s) | 251, 434, 509, 693, 786, 957 |
| MaeII | ACGT | 1 cut(s) | 107 |
| MaeIII | GTNAC | 3 cut(s) | 806, 815, 919 |
| MalI | GATC | 3 cut(s) | 40, 300, 756 |
| MboI | GATC | 3 cut(s) | 38, 298, 754 |
| MboII | GAAGA | 7 cut(s) | 208, 341, 358, 416, 498, 634, 901 |
| MluCI | AATT | 5 cut(s) | 560, 616, 759, 842, 933 |
| MlyI | GAGTC | 1 cut(s) | 596 |
| MmeI | TCCRAC | 1 cut(s) | 217 |
| MnlI | CCTC | 9 cut(s) | 143, 230, 408, 457, 538, 716, 910, 972, 981 |
| MroXI | GAANNNNTTC | 2 cut(s) | 244, 800 |
| MseI | TTAA | 3 cut(s) | 12, 338, 978 |
| MslI | CAYNNNNRTG | 2 cut(s) | 180, 813 |
| MspR9I | CCNGG | 1 cut(s) | 714 |
| Mva1269I | GAATGC | 1 cut(s) | 747 |
| MvaI | CCWGG | 1 cut(s) | 714 |
| NcoI | CCATGG | 1 cut(s) | 928 |
| NdeI | CATATG | 1 cut(s) | 583 |
| NdeII | GATC | 3 cut(s) | 38, 298, 754 |
| NlaIII | CATG | 5 cut(s) | 362, 450, 545, 691, 932 |
| NlaIV | GGNNCC | 2 cut(s) | 460, 803 |
| NmeAIII | GCCGAG | 1 cut(s) | 487 |
| NmuCI | GTSAC | 3 cut(s) | 806, 815, 919 |
| NspI | RCATGY | 2 cut(s) | 450, 545 |
| OliI | CACNNNNGTG | 1 cut(s) | 813 |
| PciI | ACATGT | 2 cut(s) | 446, 541 |
| PctI | GAATGC | 1 cut(s) | 747 |
| PdmI | GAANNNNTTC | 2 cut(s) | 244, 800 |
| PfeI | GAWTC | 3 cut(s) | 50, 128, 245 |
| PkrI | GCNGC | 1 cut(s) | 580 |
| PleI | GAGTC | 1 cut(s) | 595 |
| PpsI | GAGTC | 1 cut(s) | 595 |
| PscI | ACATGT | 2 cut(s) | 446, 541 |
| Psp6I | CCWGG | 1 cut(s) | 712 |
| PspFI | CCCAGC | 1 cut(s) | 605 |
| PspGI | CCWGG | 1 cut(s) | 712 |
| PspN4I | GGNNCC | 2 cut(s) | 460, 803 |
| RsaI | GTAC | 4 cut(s) | 110, 155, 823, 994 |
| RsaNI | GTAC | 4 cut(s) | 109, 154, 822, 993 |
| RseI | CAYNNNNRTG | 2 cut(s) | 180, 813 |
| SalI | GTCGAC | 1 cut(s) | 437 |
| SaqAI | TTAA | 3 cut(s) | 12, 338, 978 |
| SatI | GCNGC | 1 cut(s) | 579 |
| Sau3AI | GATC | 3 cut(s) | 38, 298, 754 |
| SchI | GAGTC | 1 cut(s) | 596 |
| ScrFI | CCNGG | 1 cut(s) | 714 |
| SfaNI | GCATC | 5 cut(s) | 322, 565, 781, 886, 990 |
| SfcI | CTRYAG | 1 cut(s) | 72 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 180, 813 |
| SpeI | ACTAGT | 1 cut(s) | 956 |
| Sse9I | AATT | 5 cut(s) | 560, 616, 759, 842, 933 |
| SsiI | CCGC | 1 cut(s) | 56 |
| SspMI | CTAG | 6 cut(s) | 251, 434, 509, 693, 786, 957 |
| StyD4I | CCNGG | 1 cut(s) | 712 |
| StyI | CCWWGG | 2 cut(s) | 639, 928 |
| TaaI | ACNGT | 5 cut(s) | 84, 288, 597, 821, 925 |
| TaiI | ACGT | 1 cut(s) | 110 |
| TaqI | TCGA | 4 cut(s) | 131, 297, 384, 438 |
| TasI | AATT | 5 cut(s) | 560, 616, 759, 842, 933 |
| TfiI | GAWTC | 3 cut(s) | 50, 128, 245 |
| Tru1I | TTAA | 3 cut(s) | 12, 338, 978 |
| Tru9I | TTAA | 3 cut(s) | 12, 338, 978 |
| TscAI | CASTG | 1 cut(s) | 607 |
| TseFI | GTSAC | 3 cut(s) | 806, 815, 919 |
| TseI | GCWGC | 1 cut(s) | 578 |
| Tsp45I | GTSAC | 3 cut(s) | 806, 815, 919 |
| TspDTI | ATGAA | 6 cut(s) | 141, 347, 533, 555, 752, 835 |
| TspGWI | ACGGA | 2 cut(s) | 794, 803 |
| TspRI | CASTG | 1 cut(s) | 607 |
| XapI | RAATTY | 4 cut(s) | 560, 759, 842, 933 |
| XceI | RCATGY | 2 cut(s) | 450, 545 |
| XmiI | GTMKAC | 2 cut(s) | 438, 873 |
| XmnI | GAANNNNTTC | 2 cut(s) | 244, 800 |
| XspI | CTAG | 6 cut(s) | 251, 434, 509, 693, 786, 957 |