Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 359 |
| Acc36I | ACCTGC | 2 cut(s) | 359, 436 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 565 |
| AclWI | GGATC | 1 cut(s) | 476 |
| AcsI | RAATTY | 2 cut(s) | 10, 463 |
| AcuI | CTGAAG | 1 cut(s) | 137 |
| AfaI | GTAC | 1 cut(s) | 218 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 526, 565 |
| AgeI | ACCGGT | 1 cut(s) | 760 |
| AgsI | TTSAA | 4 cut(s) | 230, 308, 471, 628 |
| AjiI | CACGTC | 2 cut(s) | 115, 717 |
| AjnI | CCWGG | 1 cut(s) | 280 |
| AleI | CACNNNNGTG | 1 cut(s) | 353 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 521, 553 |
| AluBI | AGCT | 2 cut(s) | 47, 475 |
| AluI | AGCT | 2 cut(s) | 47, 475 |
| Alw26I | GTCTC | 1 cut(s) | 121 |
| AlwI | GGATC | 1 cut(s) | 476 |
| AoxI | GGCC | 1 cut(s) | 109 |
| ApeKI | GCWGC | 2 cut(s) | 44, 271 |
| ApoI | RAATTY | 2 cut(s) | 10, 463 |
| AsiGI | ACCGGT | 1 cut(s) | 760 |
| AspLEI | GCGC | 1 cut(s) | 276 |
| AsuHPI | GGTGA | 3 cut(s) | 82, 170, 750 |
| BaeGI | GKGCMC | 1 cut(s) | 57 |
| BbsI | GAAGAC | 1 cut(s) | 711 |
| BbvCI | CCTCAGC | 1 cut(s) | 366 |
| BbvI | GCAGC | 2 cut(s) | 56, 258 |
| BccI | CCATC | 1 cut(s) | 14 |
| BciT130I | CCWGG | 1 cut(s) | 282 |
| BclI | TGATCA | 1 cut(s) | 199 |
| BcoDI | GTCTC | 1 cut(s) | 121 |
| BfaI | CTAG | 1 cut(s) | 476 |
| BfuAI | ACCTGC | 2 cut(s) | 359, 436 |
| BisI | GCNGC | 2 cut(s) | 45, 272 |
| BlsI | GCNGC | 2 cut(s) | 46, 273 |
| BmcAI | AGTACT | 1 cut(s) | 218 |
| Bme1390I | CCNGG | 1 cut(s) | 282 |
| BmgBI | CACGTC | 2 cut(s) | 115, 717 |
| BmrFI | CCNGG | 1 cut(s) | 282 |
| BmsI | GCATC | 1 cut(s) | 223 |
| BpiI | GAAGAC | 1 cut(s) | 711 |
| BpmI | CTGGAG | 2 cut(s) | 69, 159 |
| Bpu10I | CCTNAGC | 1 cut(s) | 366 |
| BsaJI | CCNNGG | 2 cut(s) | 546, 559 |
| BsaWI | WCCGGW | 1 cut(s) | 760 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 521, 551 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 526, 565 |
| Bse118I | RCCGGY | 1 cut(s) | 760 |
| BseBI | CCWGG | 1 cut(s) | 282 |
| BseDI | CCNNGG | 2 cut(s) | 546, 559 |
| BseGI | GGATG | 1 cut(s) | 25 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 526, 565 |
| BseMII | CTCAG | 1 cut(s) | 357 |
| BseRI | GAGGAG | 2 cut(s) | 50, 606 |
| BseSI | GKGCMC | 1 cut(s) | 57 |
| BseXI | GCAGC | 2 cut(s) | 56, 258 |
| BshFI | GGCC | 1 cut(s) | 111 |
| BshTI | ACCGGT | 1 cut(s) | 760 |
| BsiSI | CCGG | 1 cut(s) | 761 |
| BslFI | GGGAC | 1 cut(s) | 583 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 526, 565 |
| BsmAI | GTCTC | 1 cut(s) | 121 |
| BsmBI | CGTCTC | 1 cut(s) | 121 |
| BsmFI | GGGAC | 1 cut(s) | 583 |
| BsnI | GGCC | 1 cut(s) | 111 |
| Bsp1286I | GDGCHC | 1 cut(s) | 57 |
| Bsp143I | GATC | 3 cut(s) | 199, 481, 661 |
| BspANI | GGCC | 1 cut(s) | 111 |
| BspCNI | CTCAG | 1 cut(s) | 358 |
| BspMI | ACCTGC | 2 cut(s) | 359, 436 |
| BspPI | GGATC | 1 cut(s) | 476 |
| BsrFI | RCCGGY | 1 cut(s) | 760 |
| BssAI | RCCGGY | 1 cut(s) | 760 |
| BssECI | CCNNGG | 2 cut(s) | 546, 559 |
| BssMI | GATC | 3 cut(s) | 199, 481, 661 |
| BssT1I | CCWWGG | 1 cut(s) | 546 |
| Bst2UI | CCWGG | 1 cut(s) | 282 |
| Bst4CI | ACNGT | 2 cut(s) | 537, 704 |
| Bst6I | CTCTTC | 1 cut(s) | 303 |
| BstC8I | GCNNGC | 3 cut(s) | 278, 650, 672 |
| BstDEI | CTNAG | 2 cut(s) | 366, 738 |
| BstDSI | CCRYGG | 1 cut(s) | 559 |
| BstF5I | GGATG | 1 cut(s) | 25 |
| BstHHI | GCGC | 1 cut(s) | 276 |
| BstKTI | GATC | 3 cut(s) | 202, 484, 664 |
| BstMAI | GTCTC | 1 cut(s) | 121 |
| BstMBI | GATC | 3 cut(s) | 199, 481, 661 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 277 |
| BstNI | CCWGG | 1 cut(s) | 282 |
| BstNSI | RCATGY | 1 cut(s) | 674 |
| BstSCI | CCNGG | 1 cut(s) | 280 |
| BstSLI | GKGCMC | 1 cut(s) | 57 |
| BstV1I | GCAGC | 2 cut(s) | 56, 258 |
| BstV2I | GAAGAC | 1 cut(s) | 711 |
| BsuRI | GGCC | 1 cut(s) | 111 |
| BtgI | CCRYGG | 1 cut(s) | 559 |
| BtrI | CACGTC | 2 cut(s) | 115, 717 |
| BtsCI | GGATG | 1 cut(s) | 25 |
| BtsIMutI | CAGTG | 3 cut(s) | 21, 700, 715 |
| BveI | ACCTGC | 2 cut(s) | 359, 436 |
| Cac8I | GCNNGC | 3 cut(s) | 278, 650, 672 |
| CfoI | GCGC | 1 cut(s) | 276 |
| Cfr10I | RCCGGY | 1 cut(s) | 760 |
| Csp6I | GTAC | 1 cut(s) | 217 |
| CspAI | ACCGGT | 1 cut(s) | 760 |
| CviAII | CATG | 2 cut(s) | 671, 687 |
| CviJI | RGCY | 4 cut(s) | 47, 111, 137, 475 |
| CviKI_1 | RGCY | 4 cut(s) | 47, 111, 137, 475 |
| CviQI | GTAC | 1 cut(s) | 217 |
| DdeI | CTNAG | 2 cut(s) | 366, 738 |
| DpnI | GATC | 3 cut(s) | 201, 483, 663 |
| DpnII | GATC | 3 cut(s) | 199, 481, 661 |
| DraI | TTTAAA | 1 cut(s) | 607 |
| Eam1104I | CTCTTC | 1 cut(s) | 303 |
| EarI | CTCTTC | 1 cut(s) | 303 |
| Eco130I | CCWWGG | 1 cut(s) | 546 |
| Eco57I | CTGAAG | 1 cut(s) | 137 |
| EcoRII | CCWGG | 1 cut(s) | 280 |
| EcoT14I | CCWWGG | 1 cut(s) | 546 |
| ErhI | CCWWGG | 1 cut(s) | 546 |
| Esp3I | CGTCTC | 1 cut(s) | 121 |
| FaeI | CATG | 2 cut(s) | 674, 690 |
| FaqI | GGGAC | 1 cut(s) | 583 |
| FatI | CATG | 2 cut(s) | 670, 686 |
| FbaI | TGATCA | 1 cut(s) | 199 |
| Fnu4HI | GCNGC | 2 cut(s) | 45, 272 |
| FokI | GGATG | 1 cut(s) | 32 |
| Fsp4HI | GCNGC | 2 cut(s) | 45, 272 |
| FspBI | CTAG | 1 cut(s) | 476 |
| GlaI | GCGC | 1 cut(s) | 275 |
| GluI | GCNGC | 2 cut(s) | 45, 272 |
| GsuI | CTGGAG | 2 cut(s) | 69, 159 |
| HaeIII | GGCC | 1 cut(s) | 111 |
| HapII | CCGG | 1 cut(s) | 761 |
| HhaI | GCGC | 1 cut(s) | 276 |
| Hin1II | CATG | 2 cut(s) | 674, 690 |
| Hin6I | GCGC | 1 cut(s) | 274 |
| HinP1I | GCGC | 1 cut(s) | 274 |
| HinfI | GANTC | 1 cut(s) | 571 |
| HpaII | CCGG | 1 cut(s) | 761 |
| HphI | GGTGA | 3 cut(s) | 82, 170, 750 |
| Hpy166II | GTNNAC | 3 cut(s) | 315, 698, 758 |
| Hpy188I | TCNGA | 2 cut(s) | 204, 499 |
| Hpy188III | TCNNGA | 4 cut(s) | 227, 263, 305, 628 |
| Hpy8I | GTNNAC | 3 cut(s) | 315, 698, 758 |
| HpyAV | CCTTC | 2 cut(s) | 101, 448 |
| HpyCH4III | ACNGT | 2 cut(s) | 537, 704 |
| HpyCH4IV | ACGT | 2 cut(s) | 114, 716 |
| HpyCH4V | TGCA | 4 cut(s) | 44, 431, 439, 638 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 277 |
| HpyF3I | CTNAG | 2 cut(s) | 366, 738 |
| HpySE526I | ACGT | 2 cut(s) | 114, 716 |
| Hsp92II | CATG | 2 cut(s) | 674, 690 |
| HspAI | GCGC | 1 cut(s) | 274 |
| Ksp22I | TGATCA | 1 cut(s) | 199 |
| Kzo9I | GATC | 3 cut(s) | 199, 481, 661 |
| Lsp1109I | GCAGC | 2 cut(s) | 56, 258 |
| LweI | GCATC | 1 cut(s) | 223 |
| MaeI | CTAG | 1 cut(s) | 476 |
| MaeII | ACGT | 2 cut(s) | 114, 716 |
| MalI | GATC | 3 cut(s) | 201, 483, 663 |
| MboI | GATC | 3 cut(s) | 199, 481, 661 |
| MboII | GAAGA | 3 cut(s) | 242, 320, 711 |
| MhlI | GDGCHC | 1 cut(s) | 57 |
| MluCI | AATT | 6 cut(s) | 10, 65, 131, 250, 463, 552 |
| MlyI | GAGTC | 1 cut(s) | 565 |
| MnlI | CCTC | 7 cut(s) | 28, 44, 312, 334, 361, 627, 733 |
| MseI | TTAA | 2 cut(s) | 416, 606 |
| MslI | CAYNNNNRTG | 1 cut(s) | 353 |
| MspA1I | CMGCKG | 1 cut(s) | 47 |
| MspI | CCGG | 1 cut(s) | 761 |
| MspR9I | CCNGG | 1 cut(s) | 282 |
| MvaI | CCWGG | 1 cut(s) | 282 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 277 |
| NdeII | GATC | 3 cut(s) | 199, 481, 661 |
| NlaIII | CATG | 2 cut(s) | 674, 690 |
| NspI | RCATGY | 1 cut(s) | 674 |
| OliI | CACNNNNGTG | 1 cut(s) | 353 |
| PaeI | GCATGC | 1 cut(s) | 674 |
| PaqCI | CACCTGC | 1 cut(s) | 359 |
| PflFI | GACNNNGTC | 1 cut(s) | 688 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 565 |
| PinAI | ACCGGT | 1 cut(s) | 760 |
| PkrI | GCNGC | 2 cut(s) | 46, 273 |
| PleI | GAGTC | 1 cut(s) | 565 |
| PpsI | GAGTC | 1 cut(s) | 565 |
| Psp6I | CCWGG | 1 cut(s) | 280 |
| PspGI | CCWGG | 1 cut(s) | 280 |
| PsyI | GACNNNGTC | 1 cut(s) | 688 |
| PvuII | CAGCTG | 1 cut(s) | 47 |
| RsaI | GTAC | 1 cut(s) | 218 |
| RsaNI | GTAC | 1 cut(s) | 217 |
| RseI | CAYNNNNRTG | 1 cut(s) | 353 |
| SaqAI | TTAA | 2 cut(s) | 416, 606 |
| SatI | GCNGC | 2 cut(s) | 45, 272 |
| Sau3AI | GATC | 3 cut(s) | 199, 481, 661 |
| ScaI | AGTACT | 1 cut(s) | 218 |
| SchI | GAGTC | 1 cut(s) | 565 |
| ScrFI | CCNGG | 1 cut(s) | 282 |
| SduI | GDGCHC | 1 cut(s) | 57 |
| SfaNI | GCATC | 1 cut(s) | 223 |
| SgrAI | CRCCGGYG | 1 cut(s) | 760 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 353 |
| SphI | GCATGC | 1 cut(s) | 674 |
| Sse9I | AATT | 6 cut(s) | 10, 65, 131, 250, 463, 552 |
| SspI | AATATT | 1 cut(s) | 146 |
| SspMI | CTAG | 1 cut(s) | 476 |
| StyD4I | CCNGG | 1 cut(s) | 280 |
| StyI | CCWWGG | 1 cut(s) | 546 |
| TaaI | ACNGT | 2 cut(s) | 537, 704 |
| TaiI | ACGT | 2 cut(s) | 117, 719 |
| TaqI | TCGA | 1 cut(s) | 664 |
| TasI | AATT | 6 cut(s) | 10, 65, 131, 250, 463, 552 |
| TatI | WGTACW | 1 cut(s) | 216 |
| Tru1I | TTAA | 2 cut(s) | 416, 606 |
| Tru9I | TTAA | 2 cut(s) | 416, 606 |
| TscAI | CASTG | 3 cut(s) | 21, 707, 715 |
| TseI | GCWGC | 2 cut(s) | 44, 271 |
| TspDTI | ATGAA | 3 cut(s) | 42, 248, 733 |
| TspGWI | ACGGA | 1 cut(s) | 576 |
| TspRI | CASTG | 3 cut(s) | 21, 707, 715 |
| Tth111I | GACNNNGTC | 1 cut(s) | 688 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 565 |
| XapI | RAATTY | 2 cut(s) | 10, 463 |
| XceI | RCATGY | 1 cut(s) | 674 |
| XspI | CTAG | 1 cut(s) | 476 |
| ZrmI | AGTACT | 1 cut(s) | 218 |