Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 462 |
| Acc16I | TGCGCA | 1 cut(s) | 743 |
| AclWI | GGATC | 3 cut(s) | 306, 686, 803 |
| AcsI | RAATTY | 4 cut(s) | 109, 137, 830, 921 |
| AcuI | CTGAAG | 1 cut(s) | 789 |
| AcyI | GRCGYC | 1 cut(s) | 459 |
| AfaI | GTAC | 2 cut(s) | 270, 530 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 226, 232, 679, 818 |
| AflIII | ACRYGT | 1 cut(s) | 535 |
| AgsI | TTSAA | 2 cut(s) | 752, 880 |
| AhlI | ACTAGT | 1 cut(s) | 5 |
| AjnI | CCWGG | 1 cut(s) | 225 |
| AluBI | AGCT | 6 cut(s) | 364, 401, 413, 555, 785, 789 |
| AluI | AGCT | 6 cut(s) | 364, 401, 413, 555, 785, 789 |
| Alw26I | GTCTC | 2 cut(s) | 14, 600 |
| AlwI | GGATC | 3 cut(s) | 306, 686, 803 |
| ApoI | RAATTY | 4 cut(s) | 109, 137, 830, 921 |
| AspLEI | GCGC | 1 cut(s) | 744 |
| AsuHPI | GGTGA | 2 cut(s) | 523, 901 |
| AsuNHI | GCTAGC | 1 cut(s) | 785 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 429, 462 |
| BauI | CACGAG | 1 cut(s) | 708 |
| BciT130I | CCWGG | 1 cut(s) | 227 |
| BciVI | GTATCC | 2 cut(s) | 146, 877 |
| BcoDI | GTCTC | 2 cut(s) | 14, 600 |
| BcuI | ACTAGT | 1 cut(s) | 5 |
| BfaI | CTAG | 3 cut(s) | 6, 786, 842 |
| BfmI | CTRYAG | 1 cut(s) | 935 |
| BfuI | GTATCC | 2 cut(s) | 146, 877 |
| Bme1390I | CCNGG | 1 cut(s) | 227 |
| BmrFI | CCNGG | 1 cut(s) | 227 |
| BmsI | GCATC | 4 cut(s) | 43, 753, 769, 778 |
| BmtI | GCTAGC | 1 cut(s) | 789 |
| BpmI | CTGGAG | 2 cut(s) | 258, 624 |
| Bsa29I | ATCGAT | 1 cut(s) | 44 |
| BsaHI | GRCGYC | 1 cut(s) | 459 |
| BsaI | GGTCTC | 1 cut(s) | 600 |
| BsaJI | CCNNGG | 3 cut(s) | 636, 864, 916 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 226, 232, 679, 818 |
| Bse118I | RCCGGY | 1 cut(s) | 308 |
| Bse1I | ACTGG | 1 cut(s) | 620 |
| Bse3DI | GCAATG | 1 cut(s) | 363 |
| BseBI | CCWGG | 1 cut(s) | 227 |
| BseCI | ATCGAT | 1 cut(s) | 44 |
| BseDI | CCNNGG | 3 cut(s) | 636, 864, 916 |
| BseGI | GGATG | 3 cut(s) | 58, 439, 744 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 226, 232, 679, 818 |
| BseMI | GCAATG | 1 cut(s) | 363 |
| BseMII | CTCAG | 1 cut(s) | 865 |
| BseNI | ACTGG | 1 cut(s) | 620 |
| BshVI | ATCGAT | 1 cut(s) | 44 |
| BsiSI | CCGG | 1 cut(s) | 309 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 226, 232, 679, 818 |
| BsmAI | GTCTC | 2 cut(s) | 14, 600 |
| Bso31I | GGTCTC | 1 cut(s) | 600 |
| Bsp1407I | TGTACA | 2 cut(s) | 268, 528 |
| Bsp143I | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| Bsp19I | CCATGG | 2 cut(s) | 864, 916 |
| BspCNI | CTCAG | 1 cut(s) | 864 |
| BspDI | ATCGAT | 1 cut(s) | 44 |
| BspOI | GCTAGC | 1 cut(s) | 789 |
| BspPI | GGATC | 3 cut(s) | 306, 686, 803 |
| BspTNI | GGTCTC | 1 cut(s) | 600 |
| BsrDI | GCAATG | 1 cut(s) | 363 |
| BsrFI | RCCGGY | 1 cut(s) | 308 |
| BsrGI | TGTACA | 2 cut(s) | 268, 528 |
| BsrI | ACTGG | 1 cut(s) | 620 |
| BssAI | RCCGGY | 1 cut(s) | 308 |
| BssECI | CCNNGG | 3 cut(s) | 636, 864, 916 |
| BssMI | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| BssNI | GRCGYC | 1 cut(s) | 459 |
| BssSI | CACGAG | 1 cut(s) | 708 |
| BssT1I | CCWWGG | 3 cut(s) | 636, 864, 916 |
| Bst2BI | CACGAG | 1 cut(s) | 708 |
| Bst2UI | CCWGG | 1 cut(s) | 227 |
| Bst4CI | ACNGT | 3 cut(s) | 32, 288, 913 |
| BstACI | GRCGYC | 1 cut(s) | 459 |
| BstAUI | TGTACA | 2 cut(s) | 268, 528 |
| BstC8I | GCNNGC | 1 cut(s) | 787 |
| BstDEI | CTNAG | 3 cut(s) | 319, 471, 851 |
| BstDSI | CCRYGG | 2 cut(s) | 864, 916 |
| BstEII | GGTNACC | 1 cut(s) | 907 |
| BstF5I | GGATG | 3 cut(s) | 58, 439, 744 |
| BstHHI | GCGC | 1 cut(s) | 744 |
| BstKTI | GATC | 5 cut(s) | 44, 301, 382, 694, 811 |
| BstMAI | GTCTC | 2 cut(s) | 14, 600 |
| BstMBI | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 766 |
| BstNI | CCWGG | 1 cut(s) | 227 |
| BstNSI | RCATGY | 3 cut(s) | 275, 539, 891 |
| BstPI | GGTNACC | 1 cut(s) | 907 |
| BstSCI | CCNGG | 1 cut(s) | 225 |
| BstSFI | CTRYAG | 1 cut(s) | 935 |
| BstX2I | RGATCY | 1 cut(s) | 808 |
| BstYI | RGATCY | 1 cut(s) | 808 |
| Bsu15I | ATCGAT | 1 cut(s) | 44 |
| BsuI | GTATCC | 2 cut(s) | 146, 877 |
| BsuTUI | ATCGAT | 1 cut(s) | 44 |
| BtgI | CCRYGG | 2 cut(s) | 864, 916 |
| BtsCI | GGATG | 3 cut(s) | 58, 439, 744 |
| Cac8I | GCNNGC | 1 cut(s) | 787 |
| CfoI | GCGC | 1 cut(s) | 744 |
| Cfr10I | RCCGGY | 1 cut(s) | 308 |
| ClaI | ATCGAT | 1 cut(s) | 44 |
| CseI | GACGC | 2 cut(s) | 527, 766 |
| CsiI | ACCWGGT | 1 cut(s) | 225 |
| Csp6I | GTAC | 2 cut(s) | 269, 529 |
| CviJI | RGCY | 9 cut(s) | 236, 364, 401, 413, 555, 689, 785, 789, 846 |
| CviKI_1 | RGCY | 9 cut(s) | 236, 364, 401, 413, 555, 689, 785, 789, 846 |
| CviQI | GTAC | 2 cut(s) | 269, 529 |
| DdeI | CTNAG | 3 cut(s) | 319, 471, 851 |
| DpnI | GATC | 5 cut(s) | 43, 300, 381, 693, 810 |
| DpnII | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| Eco130I | CCWWGG | 3 cut(s) | 636, 864, 916 |
| Eco31I | GGTCTC | 1 cut(s) | 600 |
| Eco57I | CTGAAG | 1 cut(s) | 789 |
| Eco91I | GGTNACC | 1 cut(s) | 907 |
| EcoO65I | GGTNACC | 1 cut(s) | 907 |
| EcoRI | GAATTC | 1 cut(s) | 137 |
| EcoRII | CCWGG | 1 cut(s) | 225 |
| EcoT14I | CCWWGG | 3 cut(s) | 636, 864, 916 |
| EcoT22I | ATGCAT | 1 cut(s) | 893 |
| ErhI | CCWWGG | 3 cut(s) | 636, 864, 916 |
| FokI | GGATG | 3 cut(s) | 65, 446, 731 |
| FspBI | CTAG | 3 cut(s) | 6, 786, 842 |
| FspI | TGCGCA | 1 cut(s) | 743 |
| GlaI | GCGC | 1 cut(s) | 743 |
| GsuI | CTGGAG | 2 cut(s) | 258, 624 |
| HapII | CCGG | 1 cut(s) | 309 |
| HgaI | GACGC | 2 cut(s) | 527, 766 |
| HhaI | GCGC | 1 cut(s) | 744 |
| Hin1I | GRCGYC | 1 cut(s) | 459 |
| Hin6I | GCGC | 1 cut(s) | 742 |
| HinP1I | GCGC | 1 cut(s) | 742 |
| HindIII | AAGCTT | 3 cut(s) | 362, 399, 411 |
| HinfI | GANTC | 3 cut(s) | 86, 128, 584 |
| HpaII | CCGG | 1 cut(s) | 309 |
| HphI | GGTGA | 2 cut(s) | 523, 901 |
| Hpy166II | GTNNAC | 2 cut(s) | 269, 735 |
| Hpy188I | TCNGA | 5 cut(s) | 143, 284, 303, 808, 858 |
| Hpy188III | TCNNGA | 4 cut(s) | 329, 603, 710, 812 |
| Hpy8I | GTNNAC | 2 cut(s) | 269, 735 |
| HpyAV | CCTTC | 2 cut(s) | 508, 874 |
| HpyCH4III | ACNGT | 3 cut(s) | 32, 288, 913 |
| HpyCH4IV | ACGT | 1 cut(s) | 459 |
| HpyCH4V | TGCA | 4 cut(s) | 56, 356, 891, 932 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 766 |
| HpyF3I | CTNAG | 3 cut(s) | 319, 471, 851 |
| HpySE526I | ACGT | 1 cut(s) | 459 |
| Hsp92I | GRCGYC | 1 cut(s) | 459 |
| HspAI | GCGC | 1 cut(s) | 742 |
| Kzo9I | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| LmnI | GCTCC | 2 cut(s) | 143, 239 |
| LweI | GCATC | 4 cut(s) | 43, 753, 769, 778 |
| MabI | ACCWGGT | 1 cut(s) | 225 |
| MaeI | CTAG | 3 cut(s) | 6, 786, 842 |
| MaeII | ACGT | 1 cut(s) | 459 |
| MaeIII | GTNAC | 2 cut(s) | 87, 907 |
| MalI | GATC | 5 cut(s) | 43, 300, 381, 693, 810 |
| MboI | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| MboII | GAAGA | 2 cut(s) | 556, 631 |
| MflI | RGATCY | 1 cut(s) | 808 |
| MluCI | AATT | 4 cut(s) | 109, 137, 830, 921 |
| MlyI | GAGTC | 2 cut(s) | 95, 578 |
| MnlI | CCTC | 6 cut(s) | 143, 230, 336, 717, 892, 898 |
| Mph1103I | ATGCAT | 1 cut(s) | 893 |
| MseI | TTAA | 2 cut(s) | 204, 405 |
| MslI | CAYNNNNRTG | 3 cut(s) | 264, 351, 774 |
| MspI | CCGG | 1 cut(s) | 309 |
| MspR9I | CCNGG | 1 cut(s) | 227 |
| MvaI | CCWGG | 1 cut(s) | 227 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 766 |
| NcoI | CCATGG | 2 cut(s) | 864, 916 |
| NdeII | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| NheI | GCTAGC | 1 cut(s) | 785 |
| NmuCI | GTSAC | 2 cut(s) | 87, 907 |
| NsbI | TGCGCA | 1 cut(s) | 743 |
| NsiI | ATGCAT | 1 cut(s) | 893 |
| NspI | RCATGY | 3 cut(s) | 275, 539, 891 |
| PciI | ACATGT | 1 cut(s) | 535 |
| PfeI | GAWTC | 1 cut(s) | 128 |
| PleI | GAGTC | 2 cut(s) | 94, 578 |
| PpsI | GAGTC | 2 cut(s) | 94, 578 |
| PscI | ACATGT | 1 cut(s) | 535 |
| Psp6I | CCWGG | 1 cut(s) | 225 |
| PspEI | GGTNACC | 1 cut(s) | 907 |
| PspGI | CCWGG | 1 cut(s) | 225 |
| PsuI | RGATCY | 1 cut(s) | 808 |
| RsaI | GTAC | 2 cut(s) | 270, 530 |
| RsaNI | GTAC | 2 cut(s) | 269, 529 |
| RseI | CAYNNNNRTG | 3 cut(s) | 264, 351, 774 |
| SaqAI | TTAA | 2 cut(s) | 204, 405 |
| Sau3AI | GATC | 5 cut(s) | 41, 298, 379, 691, 808 |
| SchI | GAGTC | 2 cut(s) | 95, 578 |
| ScrFI | CCNGG | 1 cut(s) | 227 |
| SexAI | ACCWGGT | 1 cut(s) | 225 |
| SfaNI | GCATC | 4 cut(s) | 43, 753, 769, 778 |
| SfcI | CTRYAG | 1 cut(s) | 935 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 264, 351, 774 |
| SpeI | ACTAGT | 1 cut(s) | 5 |
| Sse9I | AATT | 4 cut(s) | 109, 137, 830, 921 |
| SspMI | CTAG | 3 cut(s) | 6, 786, 842 |
| StyD4I | CCNGG | 1 cut(s) | 225 |
| StyI | CCWWGG | 3 cut(s) | 636, 864, 916 |
| TaaI | ACNGT | 3 cut(s) | 32, 288, 913 |
| TaiI | ACGT | 1 cut(s) | 462 |
| TaqI | TCGA | 3 cut(s) | 18, 44, 378 |
| TasI | AATT | 4 cut(s) | 109, 137, 830, 921 |
| TatI | WGTACW | 2 cut(s) | 268, 528 |
| TfiI | GAWTC | 1 cut(s) | 128 |
| Tru1I | TTAA | 2 cut(s) | 204, 405 |
| Tru9I | TTAA | 2 cut(s) | 204, 405 |
| TseFI | GTSAC | 2 cut(s) | 87, 907 |
| Tsp45I | GTSAC | 2 cut(s) | 87, 907 |
| TspDTI | ATGAA | 6 cut(s) | 102, 141, 341, 374, 423, 823 |
| TspGWI | ACGGA | 2 cut(s) | 65, 382 |
| XapI | RAATTY | 4 cut(s) | 109, 137, 830, 921 |
| XceI | RCATGY | 3 cut(s) | 275, 539, 891 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 819 |
| XspI | CTAG | 3 cut(s) | 6, 786, 842 |
| ZraI | GACGTC | 1 cut(s) | 460 |
| Zsp2I | ATGCAT | 1 cut(s) | 893 |