pycom13g12440

protein modification by small protein conjugation or removal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000185
Physical Location & Seq
Reverse (-)
8687182 .. 8688617
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12440.1

Sequence Viewer

Length: 663 bp
ATGGTTAGATATTCATATTCACCTCCAACTCATTACGCTCTAAAACTTGGGTCGTTTTCATTGCTTAACAAATCTTCGGCGGACAAATACGAGACGGGAGAATTTGACGCTGAAGGATACAAATGGAAACTAGTGCTCTACCCGAACGGAAACAAGAAGAAAAATGCGGAAGGCTACATCTCTGTTTACTTGGAAATGGTTGGAGCTGATTCTCTTCAGACTGTATGCGAAGTTTATGTTGATTTCAGGCTCTTTTTGCTTGATCTGAATAAAGGCATGTTCCTGGTTCTTCAAGAAAAAAGAGATGGCAAAGTAGAGTCTATATCGACGATCAAGGATGCTGTCATCTACAAGCATGTTTGGAAGGTTGAGAACTTTTCAAAGTTAGGTGATGACCTCTACAAATCAGAACCCTTCACTGAAGATAGTGCTCTATCCCAAGGCAAAATGGCGCTGGATTGGGTACTCATATTTCTCTTCACTTGGAATTGGATGATCCGGAAAAATATCCTGGTTGCAAAGTCTCCTCGACTACCAGTTGGGGTTGGCCTTAGCTTTATTTCACTGGACACTCTCGATAATTCAGATAGAGGGTTTTCGGTGAAGGATTCTTGCATAACGGAGGCCGTTGTCACAATCCGTGGAGCTGCAACAGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04340 ko04341 map04340 map04341
Pfam Domains
Protein Families
MATH

Protein Analysis

221

Amino Acids

24.86

Weight (kDa)

6.74

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 11 - 91 1.1e-16 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17380
fragaria_vesca FvH4_4g21140 FvH4_4g21140 FvH4_4g21190 FvH4_4g21293 FvH4_4g21321 FvH4_4g21321 FvH4_4g21321 FvH4_4g21850 FvH4_6g51410 FvH4_7g09650
malus_domestica MD07G1100800.v1.1 MD13G1142200.v1.1 MD13G1142800.v1.1 MD13G1142900.v1.1 MD13G1143000.v1.1 MD13G1143200.v1.1 MD13G1143500.v1.1 MD13G1144900.v1.1 MD13G1145100.v1.1 MD13G1145600.v1.1 MD16G1132700.v1.1 MD16G1132900.v1.1 MD16G1138600.v1.1 MD16G1138800.v1.1 MD16G1146500.v1.1
prunus_persica Prupe.1G107200_v2.0.a1 Prupe.1G107200_v2.0.a1 Prupe.1G107400_v2.0.a1 Prupe.1G107500_v2.0.a1 Prupe.1G107700_v2.0.a1 Prupe.1G107800_v2.0.a1 Prupe.1G107900_v2.0.a1 Prupe.1G108000_v2.0.a1 Prupe.2G123800_v2.0.a1 Prupe.7G081500_v2.0.a1 Prupe.7G081600_v2.0.a1 Prupe.7G081700_v2.0.a1
pyrus_communis pycom13g12350 pycom13g12370 pycom13g12380 pycom13g12390 pycom13g12400 pycom13g12440 pycom16g12300
rosa_chinensis RchiOBHm_Chr1g0346551 RchiOBHm_Chr1g0378981 RchiOBHm_Chr4g0402791 RchiOBHm_Chr4g0410831 RchiOBHm_Chr4g0426731 RchiOBHm_Chr4g0426761 RchiOBHm_Chr4g0426771 RchiOBHm_Chr4g0426801 RchiOBHm_Chr4g0426981 RchiOBHm_Chr4g0427031 RchiOBHm_Chr4g0427041 RchiOBHm_Chr4g0427051 RchiOBHm_Chr4g0427071 RchiOBHm_Chr4g0427081 RchiOBHm_Chr4g0428091 RchiOBHm_Chr4g0428111 RchiOBHm_Chr5g0048271
rosa_laevigata RLG00000007181 RLG00000007182 RLG00000007233 RLG00000007236 RLG00000007239 RLG00000007263 RLG00000007264 RLG00000007265 RLG00000007269 RLG00000007277 RLG00000007278 RLG00000007279 RLG00000007282 RLG00000028794
rosa_multiflora Rmu_co8499169.1_g000001 Rmu_sc0000953.1_g000004 Rmu_sc0000953.1_g000009 Rmu_sc0000953.1_g000010 Rmu_sc0000953.1_g000016 Rmu_sc0000953.1_g000021 Rmu_sc0000953.1_g000028 Rmu_sc0000953.1_g000029 Rmu_sc0001734.1_g000009 Rmu_sc0002091.1_g000024 Rmu_sc0004940.1_g000016 Rmu_sc0009599.1_g000017 Rmu_sc0010934.1_g000001 Rmu_sc0013367.1_g000001
rosa_roxburghii Rroxscaffold_4G00308240 Rroxscaffold_5G00368720 Rroxscaffold_5G00368730 Rroxscaffold_5G00368760 Rroxscaffold_5G00368770 Rroxscaffold_5G00368870 Rroxscaffold_5G00368900 Rroxscaffold_5G00368910 Rroxscaffold_5G00369810 Rroxscaffold_5G00369820
rosa_rugosa Rorug01G0181000 Rorug04G0208500 Rorug04G0214500 Rorug04G0214700 Rorug04G0214800 Rorug04G0214900 Rorug04G0215400 Rorug04G0215600 Rorug04G0215700 Rorug04G0215800 Rorug04G0218400 Rorug04G0222200 Rorug04G0222300
rosa_samantha Rh1AG200200 Rh1BG166500 Rh1CG185200 Rh1DG197300 Rh4AG264300 Rh4AG269500 Rh4AG269800 Rh4AG269900 Rh4AG270800 Rh4AG271300 Rh4AG271400 Rh4AG271600 Rh4AG279600 Rh4BG275800 Rh4BG276100 Rh4BG276200 Rh4BG277100 Rh4BG277400 Rh4BG277500 Rh4BG285000 Rh4CG290400 Rh4CG290700 Rh4CG290800 Rh4CG290900 Rh4CG291800 Rh4CG292100 Rh4CG292200 Rh4DG272800 Rh4DG273100 Rh4DG273200 Rh4DG273400 Rh4DG274300 Rh4DG274900 Rh4DG275000 Rh4DG282100 Rh7AG411500
rosa_wichuraiana Rw0G012470 Rw1G016770 Rw2G005260 Rw4G023380 Rw4G023410 Rw4G023420 Rw4G023510 Rw4G023530 Rw4G023540 Rw4G023550 Rw4G023560 Rw4G024290

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 498
AciI CCGC 2 cut(s) 80, 167
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 3 cut(s) 132, 200, 441
AfaI GTAC 1 cut(s) 465
AgsI TTSAA 2 cut(s) 293, 381
AhlI ACTAGT 1 cut(s) 130
AjnI CCWGG 2 cut(s) 282, 510
AluBI AGCT 3 cut(s) 206, 555, 647
AluI AGCT 3 cut(s) 206, 555, 647
Alw21I GWGCWC 2 cut(s) 138, 433
Alw26I GTCTC 2 cut(s) 86, 528
AlwI GGATC 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 498
AoxI GGCC 2 cut(s) 547, 624
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 1 cut(s) 454
AsuHPI GGTGA 3 cut(s) 12, 401, 613
Bbv12I GWGCWC 2 cut(s) 138, 433
BbvI GCAGC 1 cut(s) 634
BccI CCATC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 611
BciT130I CCWGG 2 cut(s) 284, 512
BciVI GTATCC 1 cut(s) 110
BcoDI GTCTC 2 cut(s) 86, 528
BcuI ACTAGT 1 cut(s) 130
BfaI CTAG 2 cut(s) 131, 661
BfoI RGCGCY 1 cut(s) 455
BfuI GTATCC 1 cut(s) 110
BisI GCNGC 1 cut(s) 648
BlsI GCNGC 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 284, 512
BmrFI CCNGG 2 cut(s) 284, 512
BmsI GCATC 1 cut(s) 328
Bpu10I CCTNAGC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 439, 640
BsaWI WCCGGW 1 cut(s) 498
Bse1I ACTGG 2 cut(s) 536, 570
Bse3DI GCAATG 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 498
BseBI CCWGG 2 cut(s) 284, 512
BseDI CCNNGG 2 cut(s) 439, 640
BseGI GGATG 2 cut(s) 343, 498
BseMI GCAATG 1 cut(s) 59
BseNI ACTGG 2 cut(s) 536, 570
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 1 cut(s) 634
BshFI GGCC 2 cut(s) 549, 626
BsiHKAI GWGCWC 2 cut(s) 138, 433
BsiSI CCGG 1 cut(s) 499
BsmAI GTCTC 2 cut(s) 86, 528
BsmBI CGTCTC 1 cut(s) 86
BsnI GGCC 2 cut(s) 549, 626
Bsp1286I GDGCHC 2 cut(s) 138, 433
Bsp13I TCCGGA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 262, 330, 495
BspACI CCGC 2 cut(s) 80, 167
BspANI GGCC 2 cut(s) 549, 626
BspEI TCCGGA 1 cut(s) 498
BspPI GGATC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 59
BsrI ACTGG 2 cut(s) 536, 570
BssECI CCNNGG 2 cut(s) 439, 640
BssMI GATC 3 cut(s) 262, 330, 495
BssT1I CCWWGG 1 cut(s) 439
Bst2UI CCWGG 2 cut(s) 284, 512
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 219, 482
BstDEI CTNAG 1 cut(s) 551
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 2 cut(s) 343, 498
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 3 cut(s) 265, 333, 498
BstMAI GTCTC 2 cut(s) 86, 528
BstMBI GATC 3 cut(s) 262, 330, 495
BstMWI GCNNNNNNNGC 2 cut(s) 256, 653
BstNI CCWGG 2 cut(s) 284, 512
BstNSI RCATGY 2 cut(s) 280, 359
BstSCI CCNGG 2 cut(s) 282, 510
BstV1I GCAGC 1 cut(s) 634
BsuI GTATCC 1 cut(s) 110
BsuRI GGCC 2 cut(s) 549, 626
BtgI CCRYGG 1 cut(s) 640
BtsCI GGATG 2 cut(s) 343, 498
BtsIMutI CAGTG 2 cut(s) 417, 563
CfoI GCGC 1 cut(s) 454
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 464
CviAII CATG 2 cut(s) 277, 356
CviJI RGCY 7 cut(s) 174, 206, 250, 549, 555, 626, 647
CviKI_1 RGCY 7 cut(s) 174, 206, 250, 549, 555, 626, 647
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 1 cut(s) 551
DpnI GATC 3 cut(s) 264, 332, 497
DpnII GATC 3 cut(s) 262, 330, 495
Eam1104I CTCTTC 2 cut(s) 219, 482
EarI CTCTTC 2 cut(s) 219, 482
EciI GGCGGA 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 439
Eco57I CTGAAG 3 cut(s) 132, 200, 441
EcoRII CCWGG 2 cut(s) 282, 510
EcoT14I CCWWGG 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 439
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 2 cut(s) 280, 359
FaiI YATR 8 cut(s) 16, 226, 237, 278, 323, 357, 470, 617
FatI CATG 2 cut(s) 276, 355
Fnu4HI GCNGC 1 cut(s) 648
FokI GGATG 2 cut(s) 350, 505
Fsp4HI GCNGC 1 cut(s) 648
FspBI CTAG 2 cut(s) 131, 661
GlaI GCGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 648
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 549, 626
HapII CCGG 1 cut(s) 499
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 454
Hin1II CATG 2 cut(s) 280, 359
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HinfI GANTC 3 cut(s) 209, 317, 608
HpaII CCGG 1 cut(s) 499
HphI GGTGA 3 cut(s) 12, 401, 613
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 219, 267, 409, 586
Hpy188III TCNNGA 3 cut(s) 293, 499, 575
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 5 cut(s) 107, 164, 358, 424, 598
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 3 cut(s) 518, 615, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 653
HpyF3I CTNAG 1 cut(s) 551
Hsp92II CATG 2 cut(s) 280, 359
HspAI GCGC 1 cut(s) 452
Kpn2I TCCGGA 1 cut(s) 498
Kzo9I GATC 3 cut(s) 262, 330, 495
LmnI GCTCC 2 cut(s) 203, 644
LpnPI CCDG 9 cut(s) 232, 269, 296, 440, 497, 512, 524, 549, 551
Lsp1109I GCAGC 1 cut(s) 634
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 131, 661
MaeIII GTNAC 1 cut(s) 631
MalI GATC 3 cut(s) 264, 332, 497
MboI GATC 3 cut(s) 262, 330, 495
MboII GAAGA 6 cut(s) 66, 169, 206, 281, 434, 469
MhlI GDGCHC 2 cut(s) 138, 433
MluCI AATT 3 cut(s) 101, 487, 580
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 50, 181
MnlI CCTC 5 cut(s) 33, 407, 537, 584, 616
MroI TCCGGA 1 cut(s) 498
MseI TTAA 1 cut(s) 66
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 2 cut(s) 284, 512
MvaI CCWGG 2 cut(s) 284, 512
MwoI GCNNNNNNNGC 2 cut(s) 256, 653
NdeII GATC 3 cut(s) 262, 330, 495
NlaIII CATG 2 cut(s) 280, 359
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 2 cut(s) 280, 359
PfeI GAWTC 2 cut(s) 209, 608
PkrI GCNGC 1 cut(s) 649
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 282, 510
PspGI CCWGG 2 cut(s) 282, 510
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 3 cut(s) 262, 330, 495
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 2 cut(s) 284, 512
SduI GDGCHC 2 cut(s) 138, 433
SetI ASST 7 cut(s) 25, 208, 369, 391, 399, 557, 649
SfaNI GCATC 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 130
Sse9I AATT 3 cut(s) 101, 487, 580
SsiI CCGC 2 cut(s) 80, 167
SspMI CTAG 2 cut(s) 131, 661
StyD4I CCNGG 2 cut(s) 282, 510
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 326, 529, 576
TasI AATT 3 cut(s) 101, 487, 580
TfiI GAWTC 2 cut(s) 209, 608
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 2 cut(s) 424, 570
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 3 cut(s) 162, 629, 635
TspRI CASTG 2 cut(s) 424, 570
XapI RAATTY 1 cut(s) 101
XceI RCATGY 2 cut(s) 280, 359
XspI CTAG 2 cut(s) 131, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.