Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 374, 865 |
| AccI | GTMKAC | 2 cut(s) | 444, 879 |
| AciI | CCGC | 1 cut(s) | 62 |
| AclWI | GGATC | 1 cut(s) | 768 |
| AcsI | RAATTY | 3 cut(s) | 566, 765, 848 |
| AfaI | GTAC | 3 cut(s) | 116, 161, 829 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 16, 232 |
| AflIII | ACRYGT | 2 cut(s) | 452, 547 |
| AgsI | TTSAA | 7 cut(s) | 110, 149, 335, 410, 792, 857, 895 |
| AjnI | CCWGG | 1 cut(s) | 718 |
| AleI | CACNNNNGTG | 2 cut(s) | 455, 819 |
| AluBI | AGCT | 3 cut(s) | 152, 242, 637 |
| AluI | AGCT | 3 cut(s) | 152, 242, 637 |
| AlwI | GGATC | 1 cut(s) | 768 |
| ApeKI | GCWGC | 1 cut(s) | 584 |
| ApoI | RAATTY | 3 cut(s) | 566, 765, 848 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 250, 806 |
| AsuHPI | GGTGA | 3 cut(s) | 51, 173, 764 |
| AxyI | CCTNAGG | 1 cut(s) | 645 |
| BbvI | GCAGC | 1 cut(s) | 571 |
| BceAI | ACGGC | 1 cut(s) | 828 |
| BciT130I | CCWGG | 1 cut(s) | 720 |
| BfaI | CTAG | 4 cut(s) | 257, 440, 515, 699 |
| BfuAI | ACCTGC | 2 cut(s) | 374, 865 |
| BisI | GCNGC | 1 cut(s) | 585 |
| BlsI | GCNGC | 1 cut(s) | 586 |
| Bme1390I | CCNGG | 1 cut(s) | 720 |
| BmiI | GGNNCC | 1 cut(s) | 466 |
| BmrFI | CCNGG | 1 cut(s) | 720 |
| BmsI | GCATC | 3 cut(s) | 571, 787, 892 |
| BpmI | CTGGAG | 1 cut(s) | 174 |
| BsaBI | GATNNNNATC | 1 cut(s) | 133 |
| BsaJI | CCNNGG | 2 cut(s) | 468, 718 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 456, 486 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 16, 232 |
| Bse1I | ACTGG | 2 cut(s) | 177, 809 |
| Bse21I | CCTNAGG | 1 cut(s) | 645 |
| Bse3DI | GCAATG | 1 cut(s) | 378 |
| Bse8I | GATNNNNATC | 1 cut(s) | 133 |
| BseBI | CCWGG | 1 cut(s) | 720 |
| BseDI | CCNNGG | 2 cut(s) | 468, 718 |
| BseGI | GGATG | 4 cut(s) | 528, 586, 656, 784 |
| BseJI | GATNNNNATC | 1 cut(s) | 133 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 16, 232 |
| BseMI | GCAATG | 1 cut(s) | 378 |
| BseMII | CTCAG | 1 cut(s) | 132 |
| BseNI | ACTGG | 2 cut(s) | 177, 809 |
| BseXI | GCAGC | 1 cut(s) | 571 |
| BseYI | CCCAGC | 1 cut(s) | 611 |
| BsgI | GTGCAG | 1 cut(s) | 402 |
| BslFI | GGGAC | 1 cut(s) | 687 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 16, 232 |
| BsmFI | GGGAC | 1 cut(s) | 687 |
| BsmI | GAATGC | 1 cut(s) | 753 |
| Bsp143I | GATC | 4 cut(s) | 44, 214, 304, 760 |
| BspACI | CCGC | 1 cut(s) | 62 |
| BspCNI | CTCAG | 1 cut(s) | 131 |
| BspLI | GGNNCC | 1 cut(s) | 466 |
| BspMI | ACCTGC | 2 cut(s) | 374, 865 |
| BspPI | GGATC | 1 cut(s) | 768 |
| BsrDI | GCAATG | 1 cut(s) | 378 |
| BsrI | ACTGG | 2 cut(s) | 177, 809 |
| BssECI | CCNNGG | 2 cut(s) | 468, 718 |
| BssMI | GATC | 4 cut(s) | 44, 214, 304, 760 |
| Bst2UI | CCWGG | 1 cut(s) | 720 |
| Bst4CI | ACNGT | 4 cut(s) | 90, 294, 603, 827 |
| BstC8I | GCNNGC | 1 cut(s) | 907 |
| BstDEI | CTNAG | 3 cut(s) | 118, 645, 798 |
| BstF5I | GGATG | 4 cut(s) | 528, 586, 656, 784 |
| BstKTI | GATC | 4 cut(s) | 47, 217, 307, 763 |
| BstMBI | GATC | 4 cut(s) | 44, 214, 304, 760 |
| BstNI | CCWGG | 1 cut(s) | 720 |
| BstNSI | RCATGY | 2 cut(s) | 456, 551 |
| BstSCI | CCNGG | 1 cut(s) | 718 |
| BstV1I | GCAGC | 1 cut(s) | 571 |
| Bsu36I | CCTNAGG | 1 cut(s) | 645 |
| BtsCI | GGATG | 4 cut(s) | 528, 586, 656, 784 |
| BtsI | GCAGTG | 1 cut(s) | 606 |
| BtsIMutI | CAGTG | 1 cut(s) | 606 |
| BveI | ACCTGC | 2 cut(s) | 374, 865 |
| Cac8I | GCNNGC | 1 cut(s) | 907 |
| Csp6I | GTAC | 3 cut(s) | 115, 160, 828 |
| CviAII | CATG | 3 cut(s) | 365, 453, 548 |
| CviJI | RGCY | 8 cut(s) | 152, 242, 467, 533, 618, 637, 698, 723 |
| CviKI_1 | RGCY | 8 cut(s) | 152, 242, 467, 533, 618, 637, 698, 723 |
| CviQI | GTAC | 3 cut(s) | 115, 160, 828 |
| DdeI | CTNAG | 3 cut(s) | 118, 645, 798 |
| DpnI | GATC | 4 cut(s) | 46, 216, 306, 762 |
| DpnII | GATC | 4 cut(s) | 44, 214, 304, 760 |
| Eco81I | CCTNAGG | 1 cut(s) | 645 |
| EcoRII | CCWGG | 1 cut(s) | 718 |
| FaeI | CATG | 3 cut(s) | 368, 456, 551 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 344, 376 |
| FaqI | GGGAC | 1 cut(s) | 687 |
| FatI | CATG | 3 cut(s) | 364, 452, 547 |
| FauNDI | CATATG | 1 cut(s) | 589 |
| FblI | GTMKAC | 2 cut(s) | 444, 879 |
| Fnu4HI | GCNGC | 1 cut(s) | 585 |
| FokI | GGATG | 4 cut(s) | 535, 593, 663, 771 |
| Fsp4HI | GCNGC | 1 cut(s) | 585 |
| FspBI | CTAG | 4 cut(s) | 257, 440, 515, 699 |
| GluI | GCNGC | 1 cut(s) | 585 |
| GsaI | CCCAGC | 1 cut(s) | 615 |
| GsuI | CTGGAG | 1 cut(s) | 174 |
| Hin1II | CATG | 3 cut(s) | 368, 456, 551 |
| HincII | GTYRAC | 1 cut(s) | 445 |
| HindII | GTYRAC | 1 cut(s) | 445 |
| HindIII | AAGCTT | 1 cut(s) | 635 |
| HinfI | GANTC | 5 cut(s) | 56, 134, 251, 338, 593 |
| HphI | GGTGA | 3 cut(s) | 51, 173, 764 |
| Hpy166II | GTNNAC | 3 cut(s) | 445, 606, 880 |
| Hpy188I | TCNGA | 6 cut(s) | 55, 121, 290, 309, 598, 727 |
| Hpy188III | TCNNGA | 2 cut(s) | 407, 758 |
| Hpy8I | GTNNAC | 3 cut(s) | 445, 606, 880 |
| Hpy99I | CGWCG | 1 cut(s) | 449 |
| HpyAV | CCTTC | 5 cut(s) | 133, 520, 550, 653, 798 |
| HpyCH4III | ACNGT | 4 cut(s) | 90, 294, 603, 827 |
| HpyCH4IV | ACGT | 1 cut(s) | 113 |
| HpyCH4V | TGCA | 5 cut(s) | 383, 543, 587, 691, 909 |
| HpyF3I | CTNAG | 3 cut(s) | 118, 645, 798 |
| HpySE526I | ACGT | 1 cut(s) | 113 |
| Hsp92II | CATG | 3 cut(s) | 368, 456, 551 |
| Kzo9I | GATC | 4 cut(s) | 44, 214, 304, 760 |
| LmnI | GCTCC | 1 cut(s) | 464 |
| Lsp1109I | GCAGC | 1 cut(s) | 571 |
| LweI | GCATC | 3 cut(s) | 571, 787, 892 |
| MaeI | CTAG | 4 cut(s) | 257, 440, 515, 699 |
| MaeII | ACGT | 1 cut(s) | 113 |
| MaeIII | GTNAC | 2 cut(s) | 812, 821 |
| MalI | GATC | 4 cut(s) | 46, 216, 306, 762 |
| MboI | GATC | 4 cut(s) | 44, 214, 304, 760 |
| MboII | GAAGA | 7 cut(s) | 214, 347, 364, 422, 504, 640, 907 |
| MluCI | AATT | 4 cut(s) | 566, 622, 765, 848 |
| MlyI | GAGTC | 1 cut(s) | 602 |
| MnlI | CCTC | 7 cut(s) | 149, 236, 414, 463, 544, 722, 916 |
| MroXI | GAANNNNTTC | 2 cut(s) | 250, 806 |
| MseI | TTAA | 2 cut(s) | 12, 344 |
| MslI | CAYNNNNRTG | 3 cut(s) | 186, 455, 819 |
| MspR9I | CCNGG | 1 cut(s) | 720 |
| Mva1269I | GAATGC | 1 cut(s) | 753 |
| MvaI | CCWGG | 1 cut(s) | 720 |
| NdeI | CATATG | 1 cut(s) | 589 |
| NdeII | GATC | 4 cut(s) | 44, 214, 304, 760 |
| NlaIII | CATG | 3 cut(s) | 368, 456, 551 |
| NlaIV | GGNNCC | 1 cut(s) | 466 |
| NmeAIII | GCCGAG | 1 cut(s) | 493 |
| NmuCI | GTSAC | 2 cut(s) | 812, 821 |
| NspI | RCATGY | 2 cut(s) | 456, 551 |
| OliI | CACNNNNGTG | 2 cut(s) | 455, 819 |
| PciI | ACATGT | 2 cut(s) | 452, 547 |
| PctI | GAATGC | 1 cut(s) | 753 |
| PdmI | GAANNNNTTC | 2 cut(s) | 250, 806 |
| PfeI | GAWTC | 4 cut(s) | 56, 134, 251, 338 |
| PkrI | GCNGC | 1 cut(s) | 586 |
| PleI | GAGTC | 1 cut(s) | 601 |
| PpsI | GAGTC | 1 cut(s) | 601 |
| PscI | ACATGT | 2 cut(s) | 452, 547 |
| Psp6I | CCWGG | 1 cut(s) | 718 |
| PspFI | CCCAGC | 1 cut(s) | 611 |
| PspGI | CCWGG | 1 cut(s) | 718 |
| PspN4I | GGNNCC | 1 cut(s) | 466 |
| RsaI | GTAC | 3 cut(s) | 116, 161, 829 |
| RsaNI | GTAC | 3 cut(s) | 115, 160, 828 |
| RseI | CAYNNNNRTG | 3 cut(s) | 186, 455, 819 |
| SalI | GTCGAC | 1 cut(s) | 443 |
| SaqAI | TTAA | 2 cut(s) | 12, 344 |
| SatI | GCNGC | 1 cut(s) | 585 |
| Sau3AI | GATC | 4 cut(s) | 44, 214, 304, 760 |
| SchI | GAGTC | 1 cut(s) | 602 |
| ScrFI | CCNGG | 1 cut(s) | 720 |
| SfaNI | GCATC | 3 cut(s) | 571, 787, 892 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 186, 455, 819 |
| Sse9I | AATT | 4 cut(s) | 566, 622, 765, 848 |
| SsiI | CCGC | 1 cut(s) | 62 |
| SspMI | CTAG | 4 cut(s) | 257, 440, 515, 699 |
| StyD4I | CCNGG | 1 cut(s) | 718 |
| TaaI | ACNGT | 4 cut(s) | 90, 294, 603, 827 |
| TaiI | ACGT | 1 cut(s) | 116 |
| TaqI | TCGA | 4 cut(s) | 137, 303, 390, 444 |
| TasI | AATT | 4 cut(s) | 566, 622, 765, 848 |
| TfiI | GAWTC | 4 cut(s) | 56, 134, 251, 338 |
| Tru1I | TTAA | 2 cut(s) | 12, 344 |
| Tru9I | TTAA | 2 cut(s) | 12, 344 |
| TscAI | CASTG | 1 cut(s) | 613 |
| TseFI | GTSAC | 2 cut(s) | 812, 821 |
| TseI | GCWGC | 1 cut(s) | 584 |
| Tsp45I | GTSAC | 2 cut(s) | 812, 821 |
| TspDTI | ATGAA | 6 cut(s) | 147, 353, 539, 561, 758, 841 |
| TspGWI | ACGGA | 2 cut(s) | 67, 809 |
| TspRI | CASTG | 1 cut(s) | 613 |
| XapI | RAATTY | 3 cut(s) | 566, 765, 848 |
| XceI | RCATGY | 2 cut(s) | 456, 551 |
| XmiI | GTMKAC | 2 cut(s) | 444, 879 |
| XmnI | GAANNNNTTC | 2 cut(s) | 250, 806 |
| XspI | CTAG | 4 cut(s) | 257, 440, 515, 699 |