Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 512 |
| AclWI | GGATC | 3 cut(s) | 75, 455, 572 |
| AcsI | RAATTY | 2 cut(s) | 599, 690 |
| AcuI | CTGAAG | 1 cut(s) | 558 |
| AfaI | GTAC | 2 cut(s) | 39, 299 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 448, 587 |
| AflIII | ACRYGT | 1 cut(s) | 304 |
| AgsI | TTSAA | 2 cut(s) | 521, 649 |
| AluBI | AGCT | 6 cut(s) | 133, 170, 182, 324, 554, 558 |
| AluI | AGCT | 6 cut(s) | 133, 170, 182, 324, 554, 558 |
| Alw26I | GTCTC | 1 cut(s) | 369 |
| AlwI | GGATC | 3 cut(s) | 75, 455, 572 |
| ApoI | RAATTY | 2 cut(s) | 599, 690 |
| AspLEI | GCGC | 1 cut(s) | 513 |
| AsuHPI | GGTGA | 2 cut(s) | 292, 670 |
| AsuNHI | GCTAGC | 1 cut(s) | 554 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 198, 231 |
| BauI | CACGAG | 1 cut(s) | 477 |
| BciVI | GTATCC | 1 cut(s) | 646 |
| BcoDI | GTCTC | 1 cut(s) | 369 |
| BfaI | CTAG | 2 cut(s) | 555, 611 |
| BfmI | CTRYAG | 1 cut(s) | 704 |
| BfuI | GTATCC | 1 cut(s) | 646 |
| BmsI | GCATC | 3 cut(s) | 522, 538, 547 |
| BmtI | GCTAGC | 1 cut(s) | 558 |
| BpmI | CTGGAG | 2 cut(s) | 27, 393 |
| BsaI | GGTCTC | 1 cut(s) | 369 |
| BsaJI | CCNNGG | 3 cut(s) | 405, 633, 685 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 448, 587 |
| Bse118I | RCCGGY | 1 cut(s) | 77 |
| Bse1I | ACTGG | 1 cut(s) | 389 |
| Bse3DI | GCAATG | 1 cut(s) | 132 |
| BseDI | CCNNGG | 3 cut(s) | 405, 633, 685 |
| BseGI | GGATG | 2 cut(s) | 208, 513 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 448, 587 |
| BseMI | GCAATG | 1 cut(s) | 132 |
| BseMII | CTCAG | 1 cut(s) | 634 |
| BseNI | ACTGG | 1 cut(s) | 389 |
| BsiSI | CCGG | 1 cut(s) | 78 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 448, 587 |
| BsmAI | GTCTC | 1 cut(s) | 369 |
| Bso31I | GGTCTC | 1 cut(s) | 369 |
| Bsp1407I | TGTACA | 2 cut(s) | 37, 297 |
| Bsp143I | GATC | 4 cut(s) | 67, 148, 460, 577 |
| Bsp19I | CCATGG | 2 cut(s) | 633, 685 |
| BspCNI | CTCAG | 1 cut(s) | 633 |
| BspOI | GCTAGC | 1 cut(s) | 558 |
| BspPI | GGATC | 3 cut(s) | 75, 455, 572 |
| BspTNI | GGTCTC | 1 cut(s) | 369 |
| BsrDI | GCAATG | 1 cut(s) | 132 |
| BsrFI | RCCGGY | 1 cut(s) | 77 |
| BsrGI | TGTACA | 2 cut(s) | 37, 297 |
| BsrI | ACTGG | 1 cut(s) | 389 |
| BssAI | RCCGGY | 1 cut(s) | 77 |
| BssECI | CCNNGG | 3 cut(s) | 405, 633, 685 |
| BssMI | GATC | 4 cut(s) | 67, 148, 460, 577 |
| BssSI | CACGAG | 1 cut(s) | 477 |
| BssT1I | CCWWGG | 3 cut(s) | 405, 633, 685 |
| Bst2BI | CACGAG | 1 cut(s) | 477 |
| Bst4CI | ACNGT | 2 cut(s) | 57, 682 |
| BstAUI | TGTACA | 2 cut(s) | 37, 297 |
| BstC8I | GCNNGC | 1 cut(s) | 556 |
| BstDEI | CTNAG | 3 cut(s) | 88, 240, 620 |
| BstDSI | CCRYGG | 2 cut(s) | 633, 685 |
| BstEII | GGTNACC | 1 cut(s) | 676 |
| BstF5I | GGATG | 2 cut(s) | 208, 513 |
| BstHHI | GCGC | 1 cut(s) | 513 |
| BstKTI | GATC | 4 cut(s) | 70, 151, 463, 580 |
| BstMAI | GTCTC | 1 cut(s) | 369 |
| BstMBI | GATC | 4 cut(s) | 67, 148, 460, 577 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 535 |
| BstNSI | RCATGY | 3 cut(s) | 44, 308, 660 |
| BstPI | GGTNACC | 1 cut(s) | 676 |
| BstSFI | CTRYAG | 1 cut(s) | 704 |
| BstX2I | RGATCY | 1 cut(s) | 577 |
| BstYI | RGATCY | 1 cut(s) | 577 |
| BsuI | GTATCC | 1 cut(s) | 646 |
| BtgI | CCRYGG | 2 cut(s) | 633, 685 |
| BtsCI | GGATG | 2 cut(s) | 208, 513 |
| Cac8I | GCNNGC | 1 cut(s) | 556 |
| CfoI | GCGC | 1 cut(s) | 513 |
| Cfr10I | RCCGGY | 1 cut(s) | 77 |
| CseI | GACGC | 2 cut(s) | 296, 535 |
| Csp6I | GTAC | 2 cut(s) | 38, 298 |
| CviJI | RGCY | 9 cut(s) | 5, 133, 170, 182, 324, 458, 554, 558, 615 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 133, 170, 182, 324, 458, 554, 558, 615 |
| CviQI | GTAC | 2 cut(s) | 38, 298 |
| DdeI | CTNAG | 3 cut(s) | 88, 240, 620 |
| DpnI | GATC | 4 cut(s) | 69, 150, 462, 579 |
| DpnII | GATC | 4 cut(s) | 67, 148, 460, 577 |
| Eco130I | CCWWGG | 3 cut(s) | 405, 633, 685 |
| Eco31I | GGTCTC | 1 cut(s) | 369 |
| Eco57I | CTGAAG | 1 cut(s) | 558 |
| Eco91I | GGTNACC | 1 cut(s) | 676 |
| EcoO65I | GGTNACC | 1 cut(s) | 676 |
| EcoT14I | CCWWGG | 3 cut(s) | 405, 633, 685 |
| EcoT22I | ATGCAT | 1 cut(s) | 662 |
| ErhI | CCWWGG | 3 cut(s) | 405, 633, 685 |
| FokI | GGATG | 2 cut(s) | 215, 500 |
| FspBI | CTAG | 2 cut(s) | 555, 611 |
| FspI | TGCGCA | 1 cut(s) | 512 |
| GlaI | GCGC | 1 cut(s) | 512 |
| GsuI | CTGGAG | 2 cut(s) | 27, 393 |
| HapII | CCGG | 1 cut(s) | 78 |
| HgaI | GACGC | 2 cut(s) | 296, 535 |
| HhaI | GCGC | 1 cut(s) | 513 |
| Hin6I | GCGC | 1 cut(s) | 511 |
| HinP1I | GCGC | 1 cut(s) | 511 |
| HindIII | AAGCTT | 3 cut(s) | 131, 168, 180 |
| HinfI | GANTC | 1 cut(s) | 353 |
| HpaII | CCGG | 1 cut(s) | 78 |
| HphI | GGTGA | 2 cut(s) | 292, 670 |
| Hpy166II | GTNNAC | 2 cut(s) | 38, 504 |
| Hpy188I | TCNGA | 4 cut(s) | 53, 72, 577, 627 |
| Hpy188III | TCNNGA | 4 cut(s) | 98, 372, 479, 581 |
| Hpy8I | GTNNAC | 2 cut(s) | 38, 504 |
| HpyAV | CCTTC | 2 cut(s) | 277, 643 |
| HpyCH4III | ACNGT | 2 cut(s) | 57, 682 |
| HpyCH4V | TGCA | 2 cut(s) | 125, 660 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 535 |
| HpyF3I | CTNAG | 3 cut(s) | 88, 240, 620 |
| HspAI | GCGC | 1 cut(s) | 511 |
| Kzo9I | GATC | 4 cut(s) | 67, 148, 460, 577 |
| LmnI | GCTCC | 1 cut(s) | 8 |
| LweI | GCATC | 3 cut(s) | 522, 538, 547 |
| MaeI | CTAG | 2 cut(s) | 555, 611 |
| MaeIII | GTNAC | 1 cut(s) | 676 |
| MalI | GATC | 4 cut(s) | 69, 150, 462, 579 |
| MboI | GATC | 4 cut(s) | 67, 148, 460, 577 |
| MboII | GAAGA | 2 cut(s) | 325, 400 |
| MflI | RGATCY | 1 cut(s) | 577 |
| MluCI | AATT | 2 cut(s) | 599, 690 |
| MlyI | GAGTC | 1 cut(s) | 347 |
| MnlI | CCTC | 5 cut(s) | 105, 220, 486, 661, 667 |
| Mph1103I | ATGCAT | 1 cut(s) | 662 |
| MseI | TTAA | 1 cut(s) | 174 |
| MslI | CAYNNNNRTG | 3 cut(s) | 33, 120, 543 |
| MspI | CCGG | 1 cut(s) | 78 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 535 |
| NcoI | CCATGG | 2 cut(s) | 633, 685 |
| NdeII | GATC | 4 cut(s) | 67, 148, 460, 577 |
| NheI | GCTAGC | 1 cut(s) | 554 |
| NmuCI | GTSAC | 1 cut(s) | 676 |
| NsbI | TGCGCA | 1 cut(s) | 512 |
| NsiI | ATGCAT | 1 cut(s) | 662 |
| NspI | RCATGY | 3 cut(s) | 44, 308, 660 |
| PciI | ACATGT | 1 cut(s) | 304 |
| PleI | GAGTC | 1 cut(s) | 347 |
| PpsI | GAGTC | 1 cut(s) | 347 |
| PscI | ACATGT | 1 cut(s) | 304 |
| PspEI | GGTNACC | 1 cut(s) | 676 |
| PsuI | RGATCY | 1 cut(s) | 577 |
| RsaI | GTAC | 2 cut(s) | 39, 299 |
| RsaNI | GTAC | 2 cut(s) | 38, 298 |
| RseI | CAYNNNNRTG | 3 cut(s) | 33, 120, 543 |
| SaqAI | TTAA | 1 cut(s) | 174 |
| Sau3AI | GATC | 4 cut(s) | 67, 148, 460, 577 |
| SchI | GAGTC | 1 cut(s) | 347 |
| SfaNI | GCATC | 3 cut(s) | 522, 538, 547 |
| SfcI | CTRYAG | 1 cut(s) | 704 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 33, 120, 543 |
| Sse9I | AATT | 2 cut(s) | 599, 690 |
| SspMI | CTAG | 2 cut(s) | 555, 611 |
| StyI | CCWWGG | 3 cut(s) | 405, 633, 685 |
| TaaI | ACNGT | 2 cut(s) | 57, 682 |
| TaqI | TCGA | 1 cut(s) | 147 |
| TasI | AATT | 2 cut(s) | 599, 690 |
| TatI | WGTACW | 2 cut(s) | 37, 297 |
| Tru1I | TTAA | 1 cut(s) | 174 |
| Tru9I | TTAA | 1 cut(s) | 174 |
| TseFI | GTSAC | 1 cut(s) | 676 |
| Tsp45I | GTSAC | 1 cut(s) | 676 |
| TspDTI | ATGAA | 4 cut(s) | 110, 143, 192, 592 |
| TspGWI | ACGGA | 1 cut(s) | 151 |
| XapI | RAATTY | 2 cut(s) | 599, 690 |
| XceI | RCATGY | 3 cut(s) | 44, 308, 660 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 588 |
| XspI | CTAG | 2 cut(s) | 555, 611 |
| Zsp2I | ATGCAT | 1 cut(s) | 662 |