Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1002 |
| Acc36I | ACCTGC | 1 cut(s) | 401 |
| AccI | GTMKAC | 2 cut(s) | 471, 1050 |
| AccII | CGCG | 1 cut(s) | 1133 |
| AciI | CCGC | 2 cut(s) | 89, 1131 |
| AclWI | GGATC | 1 cut(s) | 798 |
| AcsI | RAATTY | 4 cut(s) | 593, 614, 795, 878 |
| AcuI | CTGAAG | 1 cut(s) | 1085 |
| AcyI | GRCGYC | 1 cut(s) | 999 |
| AfaI | GTAC | 2 cut(s) | 143, 188 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 259, 866 |
| AflIII | ACRYGT | 2 cut(s) | 479, 574 |
| AjnI | CCWGG | 2 cut(s) | 748, 786 |
| AluBI | AGCT | 9 cut(s) | 33, 46, 179, 269, 667, 863, 955, 1069, 1077 |
| AluI | AGCT | 9 cut(s) | 33, 46, 179, 269, 667, 863, 955, 1069, 1077 |
| Alw21I | GWGCWC | 2 cut(s) | 859, 957 |
| Alw26I | GTCTC | 3 cut(s) | 714, 1119, 1175 |
| AlwI | GGATC | 1 cut(s) | 798 |
| ApeKI | GCWGC | 4 cut(s) | 817, 967, 970, 1106 |
| ApoI | RAATTY | 4 cut(s) | 593, 614, 795, 878 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 277 |
| AspLEI | GCGC | 1 cut(s) | 755 |
| AsuHPI | GGTGA | 2 cut(s) | 78, 200 |
| BanII | GRGCYC | 1 cut(s) | 957 |
| Bbv12I | GWGCWC | 2 cut(s) | 859, 957 |
| BbvI | GCAGC | 4 cut(s) | 829, 979, 982, 1118 |
| BceAI | ACGGC | 1 cut(s) | 973 |
| BciT130I | CCWGG | 2 cut(s) | 750, 788 |
| BcoDI | GTCTC | 3 cut(s) | 714, 1119, 1175 |
| BfaI | CTAG | 5 cut(s) | 284, 467, 542, 729, 828 |
| BfmI | CTRYAG | 1 cut(s) | 1047 |
| BfoI | RGCGCY | 1 cut(s) | 756 |
| BfuAI | ACCTGC | 1 cut(s) | 401 |
| BisI | GCNGC | 4 cut(s) | 818, 968, 971, 1107 |
| BlsI | GCNGC | 4 cut(s) | 819, 969, 972, 1108 |
| BmcAI | AGTACT | 1 cut(s) | 143 |
| Bme1390I | CCNGG | 2 cut(s) | 750, 788 |
| BmiI | GGNNCC | 2 cut(s) | 493, 845 |
| BmrFI | CCNGG | 2 cut(s) | 750, 788 |
| BmsI | GCATC | 5 cut(s) | 355, 792, 817, 829, 922 |
| BpmI | CTGGAG | 2 cut(s) | 201, 843 |
| Bpu10I | CCTNAGC | 1 cut(s) | 29 |
| BsaBI | GATNNNNATC | 1 cut(s) | 160 |
| BsaHI | GRCGYC | 1 cut(s) | 999 |
| BsaJI | CCNNGG | 3 cut(s) | 495, 748, 847 |
| BsaWI | WCCGGW | 1 cut(s) | 1086 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 233, 263, 483, 513 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 259, 866 |
| Bse1I | ACTGG | 2 cut(s) | 204, 845 |
| Bse3DI | GCAATG | 1 cut(s) | 405 |
| Bse8I | GATNNNNATC | 1 cut(s) | 160 |
| BseBI | CCWGG | 2 cut(s) | 750, 788 |
| BseDI | CCNNGG | 3 cut(s) | 495, 748, 847 |
| BseGI | GGATG | 3 cut(s) | 555, 783, 820 |
| BseJI | GATNNNNATC | 1 cut(s) | 160 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 259, 866 |
| BseMI | GCAATG | 1 cut(s) | 405 |
| BseMII | CTCAG | 1 cut(s) | 159 |
| BseNI | ACTGG | 2 cut(s) | 204, 845 |
| BseXI | GCAGC | 4 cut(s) | 829, 979, 982, 1118 |
| BseYI | CCCAGC | 1 cut(s) | 641 |
| BsgI | GTGCAG | 1 cut(s) | 429 |
| Bsh1236I | CGCG | 1 cut(s) | 1133 |
| BsiHKAI | GWGCWC | 2 cut(s) | 859, 957 |
| BsiSI | CCGG | 1 cut(s) | 1087 |
| BslFI | GGGAC | 1 cut(s) | 717 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 259, 866 |
| BsmAI | GTCTC | 3 cut(s) | 714, 1119, 1175 |
| BsmBI | CGTCTC | 1 cut(s) | 1175 |
| BsmFI | GGGAC | 1 cut(s) | 717 |
| Bsp1286I | GDGCHC | 2 cut(s) | 859, 957 |
| Bsp143I | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| BspACI | CCGC | 2 cut(s) | 89, 1131 |
| BspCNI | CTCAG | 1 cut(s) | 158 |
| BspFNI | CGCG | 1 cut(s) | 1133 |
| BspLI | GGNNCC | 2 cut(s) | 493, 845 |
| BspMI | ACCTGC | 1 cut(s) | 401 |
| BspPI | GGATC | 1 cut(s) | 798 |
| BspQI | GCTCTTC | 1 cut(s) | 978 |
| BsrDI | GCAATG | 1 cut(s) | 405 |
| BsrI | ACTGG | 2 cut(s) | 204, 845 |
| BssECI | CCNNGG | 3 cut(s) | 495, 748, 847 |
| BssMI | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| BssNI | GRCGYC | 1 cut(s) | 999 |
| Bst2UI | CCWGG | 2 cut(s) | 750, 788 |
| Bst4CI | ACNGT | 5 cut(s) | 117, 321, 633, 1143, 1169 |
| Bst6I | CTCTTC | 1 cut(s) | 978 |
| BstACI | GRCGYC | 1 cut(s) | 999 |
| BstC8I | GCNNGC | 3 cut(s) | 906, 937, 1015 |
| BstDEI | CTNAG | 2 cut(s) | 29, 145 |
| BstDSI | CCRYGG | 1 cut(s) | 847 |
| BstF5I | GGATG | 3 cut(s) | 555, 783, 820 |
| BstFNI | CGCG | 1 cut(s) | 1133 |
| BstH2I | RGCGCY | 1 cut(s) | 756 |
| BstHHI | GCGC | 1 cut(s) | 755 |
| BstKTI | GATC | 4 cut(s) | 74, 334, 793, 1098 |
| BstMAI | GTCTC | 3 cut(s) | 714, 1119, 1175 |
| BstMBI | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 814, 1112 |
| BstNI | CCWGG | 2 cut(s) | 750, 788 |
| BstNSI | RCATGY | 2 cut(s) | 483, 578 |
| BstSCI | CCNGG | 2 cut(s) | 748, 786 |
| BstSFI | CTRYAG | 1 cut(s) | 1047 |
| BstUI | CGCG | 1 cut(s) | 1133 |
| BstV1I | GCAGC | 4 cut(s) | 829, 979, 982, 1118 |
| BtgI | CCRYGG | 1 cut(s) | 847 |
| BtsCI | GGATG | 3 cut(s) | 555, 783, 820 |
| BtsI | GCAGTG | 2 cut(s) | 636, 859 |
| BtsIMutI | CAGTG | 2 cut(s) | 636, 859 |
| BveI | ACCTGC | 1 cut(s) | 401 |
| Cac8I | GCNNGC | 3 cut(s) | 906, 937, 1015 |
| CfoI | GCGC | 1 cut(s) | 755 |
| Csp6I | GTAC | 2 cut(s) | 142, 187 |
| CviAII | CATG | 3 cut(s) | 392, 480, 575 |
| CviQI | GTAC | 2 cut(s) | 142, 187 |
| DdeI | CTNAG | 2 cut(s) | 29, 145 |
| DpnI | GATC | 4 cut(s) | 73, 333, 792, 1097 |
| DpnII | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| Eam1104I | CTCTTC | 1 cut(s) | 978 |
| EarI | CTCTTC | 1 cut(s) | 978 |
| Ecl136II | GAGCTC | 1 cut(s) | 955 |
| Eco24I | GRGCYC | 1 cut(s) | 957 |
| Eco53kI | GAGCTC | 1 cut(s) | 955 |
| Eco57I | CTGAAG | 1 cut(s) | 1085 |
| EcoICRI | GAGCTC | 1 cut(s) | 955 |
| EcoRII | CCWGG | 2 cut(s) | 748, 786 |
| EcoT38I | GRGCYC | 1 cut(s) | 957 |
| Esp3I | CGTCTC | 1 cut(s) | 1175 |
| FaeI | CATG | 3 cut(s) | 395, 483, 578 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 371, 403 |
| FaqI | GGGAC | 1 cut(s) | 717 |
| FatI | CATG | 3 cut(s) | 391, 479, 574 |
| FblI | GTMKAC | 2 cut(s) | 471, 1050 |
| Fnu4HI | GCNGC | 4 cut(s) | 818, 968, 971, 1107 |
| FokI | GGATG | 3 cut(s) | 562, 770, 807 |
| FriOI | GRGCYC | 1 cut(s) | 957 |
| Fsp4HI | GCNGC | 4 cut(s) | 818, 968, 971, 1107 |
| FspBI | CTAG | 5 cut(s) | 284, 467, 542, 729, 828 |
| GlaI | GCGC | 1 cut(s) | 754 |
| GluI | GCNGC | 4 cut(s) | 818, 968, 971, 1107 |
| GsaI | CCCAGC | 1 cut(s) | 645 |
| GsuI | CTGGAG | 2 cut(s) | 201, 843 |
| HaeII | RGCGCY | 1 cut(s) | 756 |
| HapII | CCGG | 1 cut(s) | 1087 |
| HhaI | GCGC | 1 cut(s) | 755 |
| Hin1I | GRCGYC | 1 cut(s) | 999 |
| Hin1II | CATG | 3 cut(s) | 395, 483, 578 |
| Hin6I | GCGC | 1 cut(s) | 753 |
| HinP1I | GCGC | 1 cut(s) | 753 |
| HincII | GTYRAC | 1 cut(s) | 472 |
| HindII | GTYRAC | 1 cut(s) | 472 |
| HinfI | GANTC | 4 cut(s) | 83, 161, 278, 623 |
| HpaII | CCGG | 1 cut(s) | 1087 |
| HphI | GGTGA | 2 cut(s) | 78, 200 |
| Hpy166II | GTNNAC | 3 cut(s) | 472, 636, 1051 |
| Hpy188I | TCNGA | 5 cut(s) | 82, 148, 317, 336, 628 |
| Hpy188III | TCNNGA | 1 cut(s) | 434 |
| Hpy8I | GTNNAC | 3 cut(s) | 472, 636, 1051 |
| Hpy99I | CGWCG | 5 cut(s) | 994, 1001, 1004, 1024, 1138 |
| HpyAV | CCTTC | 4 cut(s) | 13, 160, 577, 834 |
| HpyCH4III | ACNGT | 5 cut(s) | 117, 321, 633, 1143, 1169 |
| HpyCH4IV | ACGT | 1 cut(s) | 999 |
| HpyCH4V | TGCA | 4 cut(s) | 410, 570, 939, 1152 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 814, 1112 |
| HpyF3I | CTNAG | 2 cut(s) | 29, 145 |
| HpySE526I | ACGT | 1 cut(s) | 999 |
| Hsp92I | GRCGYC | 1 cut(s) | 999 |
| Hsp92II | CATG | 3 cut(s) | 395, 483, 578 |
| HspAI | GCGC | 1 cut(s) | 753 |
| Kzo9I | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| LguI | GCTCTTC | 1 cut(s) | 978 |
| LmnI | GCTCC | 3 cut(s) | 491, 862, 960 |
| Lsp1109I | GCAGC | 4 cut(s) | 829, 979, 982, 1118 |
| LweI | GCATC | 5 cut(s) | 355, 792, 817, 829, 922 |
| MaeI | CTAG | 5 cut(s) | 284, 467, 542, 729, 828 |
| MaeII | ACGT | 1 cut(s) | 999 |
| MaeIII | GTNAC | 1 cut(s) | 660 |
| MalI | GATC | 4 cut(s) | 73, 333, 792, 1097 |
| MboI | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| MhlI | GDGCHC | 2 cut(s) | 859, 957 |
| MluCI | AATT | 9 cut(s) | 593, 614, 652, 722, 795, 878, 1030, 1147, 1162 |
| MlyI | GAGTC | 1 cut(s) | 632 |
| MmeI | TCCRAC | 2 cut(s) | 982, 1102 |
| MnlI | CCTC | 8 cut(s) | 176, 263, 441, 490, 571, 688, 752, 1126 |
| MroXI | GAANNNNTTC | 1 cut(s) | 277 |
| MseI | TTAA | 6 cut(s) | 12, 371, 834, 876, 1029, 1146 |
| MslI | CAYNNNNRTG | 1 cut(s) | 213 |
| MspI | CCGG | 1 cut(s) | 1087 |
| MspR9I | CCNGG | 2 cut(s) | 750, 788 |
| MvaI | CCWGG | 2 cut(s) | 750, 788 |
| MvnI | CGCG | 1 cut(s) | 1133 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 814, 1112 |
| NdeII | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| NlaIII | CATG | 3 cut(s) | 395, 483, 578 |
| NlaIV | GGNNCC | 2 cut(s) | 493, 845 |
| NmeAIII | GCCGAG | 1 cut(s) | 520 |
| NspI | RCATGY | 2 cut(s) | 483, 578 |
| PciI | ACATGT | 2 cut(s) | 479, 574 |
| PciSI | GCTCTTC | 1 cut(s) | 978 |
| PdmI | GAANNNNTTC | 1 cut(s) | 277 |
| PfeI | GAWTC | 3 cut(s) | 83, 161, 278 |
| PfoI | TCCNGGA | 1 cut(s) | 786 |
| PkrI | GCNGC | 4 cut(s) | 819, 969, 972, 1108 |
| PleI | GAGTC | 1 cut(s) | 631 |
| PpsI | GAGTC | 1 cut(s) | 631 |
| PscI | ACATGT | 2 cut(s) | 479, 574 |
| Psp124BI | GAGCTC | 1 cut(s) | 957 |
| Psp6I | CCWGG | 2 cut(s) | 748, 786 |
| PspFI | CCCAGC | 1 cut(s) | 641 |
| PspGI | CCWGG | 2 cut(s) | 748, 786 |
| PspN4I | GGNNCC | 2 cut(s) | 493, 845 |
| RsaI | GTAC | 2 cut(s) | 143, 188 |
| RsaNI | GTAC | 2 cut(s) | 142, 187 |
| RseI | CAYNNNNRTG | 1 cut(s) | 213 |
| SacI | GAGCTC | 1 cut(s) | 957 |
| SalI | GTCGAC | 1 cut(s) | 470 |
| SapI | GCTCTTC | 1 cut(s) | 978 |
| SaqAI | TTAA | 6 cut(s) | 12, 371, 834, 876, 1029, 1146 |
| SatI | GCNGC | 4 cut(s) | 818, 968, 971, 1107 |
| Sau3AI | GATC | 4 cut(s) | 71, 331, 790, 1095 |
| ScaI | AGTACT | 1 cut(s) | 143 |
| SchI | GAGTC | 1 cut(s) | 632 |
| ScrFI | CCNGG | 2 cut(s) | 750, 788 |
| SduI | GDGCHC | 2 cut(s) | 859, 957 |
| SfaNI | GCATC | 5 cut(s) | 355, 792, 817, 829, 922 |
| SfcI | CTRYAG | 1 cut(s) | 1047 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 213 |
| Sse9I | AATT | 9 cut(s) | 593, 614, 652, 722, 795, 878, 1030, 1147, 1162 |
| SsiI | CCGC | 2 cut(s) | 89, 1131 |
| SspMI | CTAG | 5 cut(s) | 284, 467, 542, 729, 828 |
| SstI | GAGCTC | 1 cut(s) | 957 |
| StyD4I | CCNGG | 2 cut(s) | 748, 786 |
| TaaI | ACNGT | 5 cut(s) | 117, 321, 633, 1143, 1169 |
| TaiI | ACGT | 1 cut(s) | 1002 |
| TaqI | TCGA | 5 cut(s) | 164, 330, 417, 471, 1019 |
| TasI | AATT | 9 cut(s) | 593, 614, 652, 722, 795, 878, 1030, 1147, 1162 |
| TatI | WGTACW | 1 cut(s) | 141 |
| TfiI | GAWTC | 3 cut(s) | 83, 161, 278 |
| Tru1I | TTAA | 6 cut(s) | 12, 371, 834, 876, 1029, 1146 |
| Tru9I | TTAA | 6 cut(s) | 12, 371, 834, 876, 1029, 1146 |
| TscAI | CASTG | 2 cut(s) | 643, 859 |
| TseI | GCWGC | 4 cut(s) | 817, 967, 970, 1106 |
| TspDTI | ATGAA | 6 cut(s) | 174, 380, 566, 588, 788, 871 |
| TspGWI | ACGGA | 2 cut(s) | 94, 836 |
| TspRI | CASTG | 2 cut(s) | 643, 859 |
| XapI | RAATTY | 4 cut(s) | 593, 614, 795, 878 |
| XceI | RCATGY | 2 cut(s) | 483, 578 |
| XmiI | GTMKAC | 2 cut(s) | 471, 1050 |
| XmnI | GAANNNNTTC | 1 cut(s) | 277 |
| XspI | CTAG | 5 cut(s) | 284, 467, 542, 729, 828 |
| ZraI | GACGTC | 1 cut(s) | 1000 |
| ZrmI | AGTACT | 1 cut(s) | 143 |