Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 140 |
| Acc36I | ACCTGC | 2 cut(s) | 140, 217 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 346 |
| AciI | CCGC | 1 cut(s) | 551 |
| AclWI | GGATC | 1 cut(s) | 257 |
| AfaI | GTAC | 2 cut(s) | 221, 625 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 111, 307, 346, 609 |
| AgsI | TTSAA | 3 cut(s) | 89, 252, 409 |
| AjnI | CCWGG | 1 cut(s) | 61 |
| AleI | CACNNNNGTG | 1 cut(s) | 134 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 302, 334 |
| AluBI | AGCT | 1 cut(s) | 256 |
| AluI | AGCT | 1 cut(s) | 256 |
| AlwI | GGATC | 1 cut(s) | 257 |
| ApeKI | GCWGC | 1 cut(s) | 52 |
| AspLEI | GCGC | 1 cut(s) | 57 |
| AsuHPI | GGTGA | 1 cut(s) | 592 |
| BbsI | GAAGAC | 1 cut(s) | 492 |
| BbvCI | CCTCAGC | 2 cut(s) | 147, 594 |
| BbvI | GCAGC | 1 cut(s) | 39 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 30 |
| BciT130I | CCWGG | 1 cut(s) | 63 |
| BclI | TGATCA | 1 cut(s) | 567 |
| BfaI | CTAG | 1 cut(s) | 257 |
| BfmI | CTRYAG | 1 cut(s) | 627 |
| BfuAI | ACCTGC | 2 cut(s) | 140, 217 |
| BisI | GCNGC | 1 cut(s) | 53 |
| BlsI | GCNGC | 1 cut(s) | 54 |
| BmcAI | AGTACT | 1 cut(s) | 625 |
| Bme1390I | CCNGG | 1 cut(s) | 63 |
| BmrFI | CCNGG | 1 cut(s) | 63 |
| BmsI | GCATC | 1 cut(s) | 4 |
| BpiI | GAAGAC | 1 cut(s) | 492 |
| BpmI | CTGGAG | 2 cut(s) | 608, 640 |
| Bpu10I | CCTNAGC | 2 cut(s) | 147, 594 |
| BsaBI | GATNNNNATC | 1 cut(s) | 566 |
| BsaJI | CCNNGG | 3 cut(s) | 327, 340, 608 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 302, 332 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 111, 307, 346, 609 |
| Bse8I | GATNNNNATC | 1 cut(s) | 566 |
| BseBI | CCWGG | 1 cut(s) | 63 |
| BseDI | CCNNGG | 3 cut(s) | 327, 340, 608 |
| BseGI | GGATG | 1 cut(s) | 567 |
| BseJI | GATNNNNATC | 1 cut(s) | 566 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 111, 307, 346, 609 |
| BseMII | CTCAG | 2 cut(s) | 138, 585 |
| BseRI | GAGGAG | 1 cut(s) | 387 |
| BseXI | GCAGC | 1 cut(s) | 39 |
| BslFI | GGGAC | 1 cut(s) | 364 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 111, 307, 346, 609 |
| BsmFI | GGGAC | 1 cut(s) | 364 |
| BsmI | GAATGC | 1 cut(s) | 582 |
| Bsp1407I | TGTACA | 1 cut(s) | 219 |
| Bsp143I | GATC | 4 cut(s) | 3, 262, 442, 567 |
| Bsp19I | CCATGG | 1 cut(s) | 608 |
| BspACI | CCGC | 1 cut(s) | 551 |
| BspCNI | CTCAG | 2 cut(s) | 139, 586 |
| BspMI | ACCTGC | 2 cut(s) | 140, 217 |
| BspPI | GGATC | 1 cut(s) | 257 |
| BsrGI | TGTACA | 1 cut(s) | 219 |
| BssECI | CCNNGG | 3 cut(s) | 327, 340, 608 |
| BssMI | GATC | 4 cut(s) | 3, 262, 442, 567 |
| BssT1I | CCWWGG | 2 cut(s) | 327, 608 |
| Bst2UI | CCWGG | 1 cut(s) | 63 |
| Bst4CI | ACNGT | 2 cut(s) | 318, 485 |
| Bst6I | CTCTTC | 1 cut(s) | 84 |
| BstAUI | TGTACA | 1 cut(s) | 219 |
| BstC8I | GCNNGC | 3 cut(s) | 59, 431, 453 |
| BstDEI | CTNAG | 4 cut(s) | 10, 147, 519, 594 |
| BstDSI | CCRYGG | 2 cut(s) | 340, 608 |
| BstEII | GGTNACC | 1 cut(s) | 598 |
| BstF5I | GGATG | 1 cut(s) | 567 |
| BstHHI | GCGC | 1 cut(s) | 57 |
| BstKTI | GATC | 4 cut(s) | 6, 265, 445, 570 |
| BstMBI | GATC | 4 cut(s) | 3, 262, 442, 567 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 58 |
| BstNI | CCWGG | 1 cut(s) | 63 |
| BstNSI | RCATGY | 1 cut(s) | 455 |
| BstPI | GGTNACC | 1 cut(s) | 598 |
| BstSCI | CCNGG | 1 cut(s) | 61 |
| BstSFI | CTRYAG | 1 cut(s) | 627 |
| BstV1I | GCAGC | 1 cut(s) | 39 |
| BstV2I | GAAGAC | 1 cut(s) | 492 |
| BtgI | CCRYGG | 2 cut(s) | 340, 608 |
| BtsCI | GGATG | 1 cut(s) | 567 |
| BtsIMutI | CAGTG | 2 cut(s) | 481, 550 |
| BveI | ACCTGC | 2 cut(s) | 140, 217 |
| Cac8I | GCNNGC | 3 cut(s) | 59, 431, 453 |
| CfoI | GCGC | 1 cut(s) | 57 |
| Csp6I | GTAC | 2 cut(s) | 220, 624 |
| CviAII | CATG | 4 cut(s) | 452, 468, 496, 609 |
| CviJI | RGCY | 4 cut(s) | 76, 256, 593, 632 |
| CviKI_1 | RGCY | 4 cut(s) | 76, 256, 593, 632 |
| CviQI | GTAC | 2 cut(s) | 220, 624 |
| DdeI | CTNAG | 4 cut(s) | 10, 147, 519, 594 |
| DpnI | GATC | 4 cut(s) | 5, 264, 444, 569 |
| DpnII | GATC | 4 cut(s) | 3, 262, 442, 567 |
| DraI | TTTAAA | 1 cut(s) | 388 |
| Eam1104I | CTCTTC | 1 cut(s) | 84 |
| EarI | CTCTTC | 1 cut(s) | 84 |
| Eco130I | CCWWGG | 2 cut(s) | 327, 608 |
| Eco91I | GGTNACC | 1 cut(s) | 598 |
| EcoO65I | GGTNACC | 1 cut(s) | 598 |
| EcoRII | CCWGG | 1 cut(s) | 61 |
| EcoT14I | CCWWGG | 2 cut(s) | 327, 608 |
| ErhI | CCWWGG | 2 cut(s) | 327, 608 |
| FaeI | CATG | 4 cut(s) | 455, 471, 499, 612 |
| FaqI | GGGAC | 1 cut(s) | 364 |
| FatI | CATG | 4 cut(s) | 451, 467, 495, 608 |
| FbaI | TGATCA | 1 cut(s) | 567 |
| Fnu4HI | GCNGC | 1 cut(s) | 53 |
| FokI | GGATG | 1 cut(s) | 574 |
| Fsp4HI | GCNGC | 1 cut(s) | 53 |
| FspBI | CTAG | 1 cut(s) | 257 |
| GlaI | GCGC | 1 cut(s) | 56 |
| GluI | GCNGC | 1 cut(s) | 53 |
| GsuI | CTGGAG | 2 cut(s) | 608, 640 |
| HhaI | GCGC | 1 cut(s) | 57 |
| Hin1II | CATG | 4 cut(s) | 455, 471, 499, 612 |
| Hin6I | GCGC | 1 cut(s) | 55 |
| HinP1I | GCGC | 1 cut(s) | 55 |
| HinfI | GANTC | 2 cut(s) | 352, 650 |
| HphI | GGTGA | 1 cut(s) | 592 |
| Hpy166II | GTNNAC | 2 cut(s) | 96, 479 |
| Hpy188I | TCNGA | 1 cut(s) | 280 |
| Hpy188III | TCNNGA | 6 cut(s) | 44, 86, 409, 571, 587, 647 |
| Hpy8I | GTNNAC | 2 cut(s) | 96, 479 |
| HpyAV | CCTTC | 1 cut(s) | 229 |
| HpyCH4III | ACNGT | 2 cut(s) | 318, 485 |
| HpyCH4V | TGCA | 3 cut(s) | 212, 419, 582 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 58 |
| HpyF3I | CTNAG | 4 cut(s) | 10, 147, 519, 594 |
| Hsp92II | CATG | 4 cut(s) | 455, 471, 499, 612 |
| HspAI | GCGC | 1 cut(s) | 55 |
| Ksp22I | TGATCA | 1 cut(s) | 567 |
| Kzo9I | GATC | 4 cut(s) | 3, 262, 442, 567 |
| Lsp1109I | GCAGC | 1 cut(s) | 39 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 1 cut(s) | 257 |
| MaeIII | GTNAC | 2 cut(s) | 161, 598 |
| MalI | GATC | 4 cut(s) | 5, 264, 444, 569 |
| MboI | GATC | 4 cut(s) | 3, 262, 442, 567 |
| MboII | GAAGA | 2 cut(s) | 101, 492 |
| MluCI | AATT | 3 cut(s) | 31, 333, 613 |
| MlyI | GAGTC | 1 cut(s) | 346 |
| MnlI | CCTC | 7 cut(s) | 93, 115, 142, 408, 514, 583, 589 |
| MseI | TTAA | 4 cut(s) | 197, 387, 533, 635 |
| MslI | CAYNNNNRTG | 1 cut(s) | 134 |
| MspR9I | CCNGG | 1 cut(s) | 63 |
| Mva1269I | GAATGC | 1 cut(s) | 582 |
| MvaI | CCWGG | 1 cut(s) | 63 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 58 |
| NcoI | CCATGG | 1 cut(s) | 608 |
| NdeII | GATC | 4 cut(s) | 3, 262, 442, 567 |
| NlaIII | CATG | 4 cut(s) | 455, 471, 499, 612 |
| NmuCI | GTSAC | 1 cut(s) | 598 |
| NspI | RCATGY | 1 cut(s) | 455 |
| OliI | CACNNNNGTG | 1 cut(s) | 134 |
| PaeI | GCATGC | 1 cut(s) | 455 |
| PaqCI | CACCTGC | 1 cut(s) | 140 |
| PctI | GAATGC | 1 cut(s) | 582 |
| PfeI | GAWTC | 1 cut(s) | 650 |
| PflFI | GACNNNGTC | 1 cut(s) | 469 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 346 |
| PkrI | GCNGC | 1 cut(s) | 54 |
| PleI | GAGTC | 1 cut(s) | 346 |
| PpsI | GAGTC | 1 cut(s) | 346 |
| Psp6I | CCWGG | 1 cut(s) | 61 |
| PspEI | GGTNACC | 1 cut(s) | 598 |
| PspGI | CCWGG | 1 cut(s) | 61 |
| PsyI | GACNNNGTC | 1 cut(s) | 469 |
| RsaI | GTAC | 2 cut(s) | 221, 625 |
| RsaNI | GTAC | 2 cut(s) | 220, 624 |
| RseI | CAYNNNNRTG | 1 cut(s) | 134 |
| SaqAI | TTAA | 4 cut(s) | 197, 387, 533, 635 |
| SatI | GCNGC | 1 cut(s) | 53 |
| Sau3AI | GATC | 4 cut(s) | 3, 262, 442, 567 |
| ScaI | AGTACT | 1 cut(s) | 625 |
| SchI | GAGTC | 1 cut(s) | 346 |
| ScrFI | CCNGG | 1 cut(s) | 63 |
| SetI | ASST | 8 cut(s) | 134, 153, 231, 240, 258, 478, 600, 605 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SfcI | CTRYAG | 1 cut(s) | 627 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 134 |
| SphI | GCATGC | 1 cut(s) | 455 |
| Sse9I | AATT | 3 cut(s) | 31, 333, 613 |
| SsiI | CCGC | 1 cut(s) | 551 |
| SspMI | CTAG | 1 cut(s) | 257 |
| StyD4I | CCNGG | 1 cut(s) | 61 |
| StyI | CCWWGG | 2 cut(s) | 327, 608 |
| TaaI | ACNGT | 2 cut(s) | 318, 485 |
| TaqI | TCGA | 2 cut(s) | 6, 445 |
| TasI | AATT | 3 cut(s) | 31, 333, 613 |
| TatI | WGTACW | 2 cut(s) | 219, 623 |
| TfiI | GAWTC | 1 cut(s) | 650 |
| Tru1I | TTAA | 4 cut(s) | 197, 387, 533, 635 |
| Tru9I | TTAA | 4 cut(s) | 197, 387, 533, 635 |
| TscAI | CASTG | 2 cut(s) | 488, 550 |
| TseFI | GTSAC | 1 cut(s) | 598 |
| TseI | GCWGC | 1 cut(s) | 52 |
| Tsp45I | GTSAC | 1 cut(s) | 598 |
| TspDTI | ATGAA | 2 cut(s) | 29, 514 |
| TspGWI | ACGGA | 1 cut(s) | 357 |
| TspRI | CASTG | 2 cut(s) | 488, 550 |
| Tth111I | GACNNNGTC | 1 cut(s) | 469 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 346 |
| XceI | RCATGY | 1 cut(s) | 455 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 615 |
| XspI | CTAG | 1 cut(s) | 257 |
| ZrmI | AGTACT | 1 cut(s) | 625 |