Ubiquitin carboxyl-terminal hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 935 |
| Acc36I | ACCTGC | 1 cut(s) | 1265 |
| AccI | GTMKAC | 2 cut(s) | 660, 753 |
| AccII | CGCG | 1 cut(s) | 256 |
| AciI | CCGC | 5 cut(s) | 52, 147, 164, 214, 254 |
| AclWI | GGATC | 6 cut(s) | 92, 101, 208, 896, 1058, 1235 |
| AcoI | YGGCCR | 1 cut(s) | 23 |
| AcsI | RAATTY | 4 cut(s) | 682, 893, 1045, 1265 |
| AcuI | CTGAAG | 4 cut(s) | 287, 444, 703, 766 |
| AcyI | GRCGYC | 2 cut(s) | 43, 55 |
| AfaI | GTAC | 2 cut(s) | 787, 1022 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 38, 166, 376, 911 |
| AgsI | TTSAA | 3 cut(s) | 580, 767, 1053 |
| AjnI | CCWGG | 2 cut(s) | 284, 361 |
| AluBI | AGCT | 6 cut(s) | 334, 458, 584, 856, 1061, 1193 |
| AluI | AGCT | 6 cut(s) | 334, 458, 584, 856, 1061, 1193 |
| Alw26I | GTCTC | 4 cut(s) | 50, 106, 161, 686 |
| AlwI | GGATC | 6 cut(s) | 92, 101, 208, 896, 1058, 1235 |
| AoxI | GGCC | 5 cut(s) | 23, 29, 306, 328, 1099 |
| ApeKI | GCWGC | 3 cut(s) | 250, 455, 1190 |
| ApoI | RAATTY | 4 cut(s) | 682, 893, 1045, 1265 |
| AspLEI | GCGC | 1 cut(s) | 238 |
| AspS9I | GGNCC | 3 cut(s) | 307, 358, 367 |
| AsuHPI | GGTGA | 2 cut(s) | 307, 480 |
| AvaII | GGWCC | 2 cut(s) | 358, 367 |
| BalI | TGGCCA | 1 cut(s) | 25 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 701, 733 |
| BbsI | GAAGAC | 1 cut(s) | 651 |
| BbvI | GCAGC | 3 cut(s) | 237, 467, 1202 |
| BccI | CCATC | 4 cut(s) | 136, 252, 318, 1022 |
| BceAI | ACGGC | 2 cut(s) | 44, 223 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1253, 1287 |
| BciT130I | CCWGG | 2 cut(s) | 286, 363 |
| BciVI | GTATCC | 1 cut(s) | 584 |
| BcoDI | GTCTC | 4 cut(s) | 50, 106, 161, 686 |
| BfaI | CTAG | 2 cut(s) | 68, 267 |
| BfoI | RGCGCY | 1 cut(s) | 239 |
| BfuAI | ACCTGC | 1 cut(s) | 1265 |
| BfuI | GTATCC | 1 cut(s) | 584 |
| BglII | AGATCT | 1 cut(s) | 269 |
| BisI | GCNGC | 6 cut(s) | 53, 148, 251, 254, 456, 1191 |
| BlsI | GCNGC | 6 cut(s) | 54, 149, 252, 255, 457, 1192 |
| Bme1390I | CCNGG | 2 cut(s) | 286, 363 |
| Bme18I | GGWCC | 2 cut(s) | 358, 367 |
| BmgT120I | GGNCC | 3 cut(s) | 307, 358, 367 |
| BmiI | GGNNCC | 4 cut(s) | 359, 360, 368, 946 |
| BmrFI | CCNGG | 2 cut(s) | 286, 363 |
| BmrI | ACTGGG | 1 cut(s) | 386 |
| BmsI | GCATC | 1 cut(s) | 1261 |
| BmuI | ACTGGG | 1 cut(s) | 386 |
| BpiI | GAAGAC | 1 cut(s) | 651 |
| BpmI | CTGGAG | 1 cut(s) | 1116 |
| Bpu10I | CCTNAGC | 1 cut(s) | 33 |
| BpuEI | CTTGAG | 1 cut(s) | 710 |
| BsaAI | YACGTR | 1 cut(s) | 1020 |
| BsaHI | GRCGYC | 2 cut(s) | 43, 55 |
| BsaI | GGTCTC | 1 cut(s) | 106 |
| BsaJI | CCNNGG | 6 cut(s) | 26, 341, 361, 362, 948, 1244 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 936, 966 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 38, 166, 376, 911 |
| Bse1I | ACTGG | 4 cut(s) | 381, 609, 700, 1262 |
| Bse3DI | GCAATG | 2 cut(s) | 122, 499 |
| BseBI | CCWGG | 2 cut(s) | 286, 363 |
| BseDI | CCNNGG | 6 cut(s) | 26, 341, 361, 362, 948, 1244 |
| BseGI | GGATG | 3 cut(s) | 147, 562, 865 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 38, 166, 376, 911 |
| BseMI | GCAATG | 2 cut(s) | 122, 499 |
| BseMII | CTCAG | 2 cut(s) | 117, 1092 |
| BseNI | ACTGG | 4 cut(s) | 381, 609, 700, 1262 |
| BseRI | GAGGAG | 2 cut(s) | 587, 1185 |
| BseXI | GCAGC | 3 cut(s) | 237, 467, 1202 |
| BseYI | CCCAGC | 3 cut(s) | 10, 166, 584 |
| Bsh1236I | CGCG | 1 cut(s) | 256 |
| Bsh1285I | CGRYCG | 1 cut(s) | 218 |
| BshFI | GGCC | 5 cut(s) | 25, 31, 308, 330, 1101 |
| BsiEI | CGRYCG | 1 cut(s) | 218 |
| BsiSI | CCGG | 2 cut(s) | 49, 675 |
| BslFI | GGGAC | 4 cut(s) | 29, 126, 371, 380 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 38, 166, 376, 911 |
| BsmAI | GTCTC | 4 cut(s) | 50, 106, 161, 686 |
| BsmBI | CGTCTC | 1 cut(s) | 50 |
| BsmFI | GGGAC | 4 cut(s) | 29, 126, 371, 380 |
| BsnI | GGCC | 5 cut(s) | 25, 31, 308, 330, 1101 |
| Bso31I | GGTCTC | 1 cut(s) | 106 |
| Bsp19I | CCATGG | 2 cut(s) | 341, 1244 |
| BspACI | CCGC | 5 cut(s) | 52, 147, 164, 214, 254 |
| BspANI | GGCC | 5 cut(s) | 25, 31, 308, 330, 1101 |
| BspCNI | CTCAG | 2 cut(s) | 116, 1091 |
| BspFNI | CGCG | 1 cut(s) | 256 |
| BspHI | TCATGA | 1 cut(s) | 898 |
| BspLI | GGNNCC | 4 cut(s) | 359, 360, 368, 946 |
| BspMI | ACCTGC | 1 cut(s) | 1265 |
| BspPI | GGATC | 6 cut(s) | 92, 101, 208, 896, 1058, 1235 |
| BspTNI | GGTCTC | 1 cut(s) | 106 |
| BsrDI | GCAATG | 2 cut(s) | 122, 499 |
| BsrI | ACTGG | 4 cut(s) | 381, 609, 700, 1262 |
| BssECI | CCNNGG | 6 cut(s) | 26, 341, 361, 362, 948, 1244 |
| BssNI | GRCGYC | 2 cut(s) | 43, 55 |
| BssT1I | CCWWGG | 2 cut(s) | 341, 1244 |
| Bst2UI | CCWGG | 2 cut(s) | 286, 363 |
| Bst4CI | ACNGT | 3 cut(s) | 395, 416, 839 |
| BstACI | GRCGYC | 2 cut(s) | 43, 55 |
| BstBAI | YACGTR | 1 cut(s) | 1020 |
| BstC8I | GCNNGC | 3 cut(s) | 120, 174, 332 |
| BstDEI | CTNAG | 3 cut(s) | 33, 103, 1078 |
| BstDSI | CCRYGG | 3 cut(s) | 26, 341, 1244 |
| BstF5I | GGATG | 3 cut(s) | 147, 562, 865 |
| BstFNI | CGCG | 1 cut(s) | 256 |
| BstH2I | RGCGCY | 1 cut(s) | 239 |
| BstHHI | GCGC | 1 cut(s) | 238 |
| BstMAI | GTCTC | 4 cut(s) | 50, 106, 161, 686 |
| BstMCI | CGRYCG | 1 cut(s) | 218 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 49, 124, 1199 |
| BstNI | CCWGG | 2 cut(s) | 286, 363 |
| BstSCI | CCNGG | 2 cut(s) | 284, 361 |
| BstUI | CGCG | 1 cut(s) | 256 |
| BstV1I | GCAGC | 3 cut(s) | 237, 467, 1202 |
| BstV2I | GAAGAC | 1 cut(s) | 651 |
| BstX2I | RGATCY | 4 cut(s) | 93, 200, 269, 1240 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 348 |
| BstYI | RGATCY | 4 cut(s) | 93, 200, 269, 1240 |
| BsuI | GTATCC | 1 cut(s) | 584 |
| BsuRI | GGCC | 5 cut(s) | 25, 31, 308, 330, 1101 |
| BtgI | CCRYGG | 3 cut(s) | 26, 341, 1244 |
| BtgZI | GCGATG | 1 cut(s) | 271 |
| BtsCI | GGATG | 3 cut(s) | 147, 562, 865 |
| BtsI | GCAGTG | 1 cut(s) | 1089 |
| BtsIMutI | CAGTG | 1 cut(s) | 1089 |
| BveI | ACCTGC | 1 cut(s) | 1265 |
| Cac8I | GCNNGC | 3 cut(s) | 120, 174, 332 |
| CciI | TCATGA | 1 cut(s) | 898 |
| CfoI | GCGC | 1 cut(s) | 238 |
| Cfr13I | GGNCC | 3 cut(s) | 307, 358, 367 |
| CseI | GACGC | 3 cut(s) | 32, 44, 925 |
| Csp6I | GTAC | 2 cut(s) | 786, 1021 |
| CviAII | CATG | 6 cut(s) | 342, 757, 899, 911, 1068, 1245 |
| CviQI | GTAC | 2 cut(s) | 786, 1021 |
| DdeI | CTNAG | 3 cut(s) | 33, 103, 1078 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 935 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 935 |
| EaeI | YGGCCR | 1 cut(s) | 23 |
| Eco130I | CCWWGG | 2 cut(s) | 341, 1244 |
| Eco31I | GGTCTC | 1 cut(s) | 106 |
| Eco47I | GGWCC | 2 cut(s) | 358, 367 |
| Eco57I | CTGAAG | 4 cut(s) | 287, 444, 703, 766 |
| EcoO109I | RGGNCCY | 2 cut(s) | 358, 367 |
| EcoRII | CCWGG | 2 cut(s) | 284, 361 |
| EcoT14I | CCWWGG | 2 cut(s) | 341, 1244 |
| ErhI | CCWWGG | 2 cut(s) | 341, 1244 |
| Esp3I | CGTCTC | 1 cut(s) | 50 |
| FaeI | CATG | 6 cut(s) | 345, 760, 902, 914, 1071, 1248 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 923, 955 |
| FaqI | GGGAC | 4 cut(s) | 29, 126, 371, 380 |
| FatI | CATG | 6 cut(s) | 341, 756, 898, 910, 1067, 1244 |
| FblI | GTMKAC | 2 cut(s) | 660, 753 |
| Fnu4HI | GCNGC | 6 cut(s) | 53, 148, 251, 254, 456, 1191 |
| FokI | GGATG | 3 cut(s) | 154, 569, 872 |
| Fsp4HI | GCNGC | 6 cut(s) | 53, 148, 251, 254, 456, 1191 |
| FspBI | CTAG | 2 cut(s) | 68, 267 |
| GlaI | GCGC | 1 cut(s) | 237 |
| GluI | GCNGC | 6 cut(s) | 53, 148, 251, 254, 456, 1191 |
| GsaI | CCCAGC | 3 cut(s) | 14, 170, 588 |
| GsuI | CTGGAG | 1 cut(s) | 1116 |
| HaeII | RGCGCY | 1 cut(s) | 239 |
| HaeIII | GGCC | 5 cut(s) | 25, 31, 308, 330, 1101 |
| HapII | CCGG | 2 cut(s) | 49, 675 |
| HgaI | GACGC | 3 cut(s) | 32, 44, 925 |
| HhaI | GCGC | 1 cut(s) | 238 |
| Hin1I | GRCGYC | 2 cut(s) | 43, 55 |
| Hin1II | CATG | 6 cut(s) | 345, 760, 902, 914, 1071, 1248 |
| Hin6I | GCGC | 1 cut(s) | 236 |
| HinP1I | GCGC | 1 cut(s) | 236 |
| HincII | GTYRAC | 2 cut(s) | 715, 1138 |
| HindII | GTYRAC | 2 cut(s) | 715, 1138 |
| HinfI | GANTC | 4 cut(s) | 485, 518, 981, 1134 |
| HpaII | CCGG | 2 cut(s) | 49, 675 |
| HphI | GGTGA | 2 cut(s) | 307, 480 |
| Hpy166II | GTNNAC | 5 cut(s) | 661, 715, 754, 1006, 1138 |
| Hpy188III | TCNNGA | 6 cut(s) | 82, 111, 219, 267, 887, 899 |
| Hpy8I | GTNNAC | 5 cut(s) | 661, 715, 754, 1006, 1138 |
| HpyAV | CCTTC | 4 cut(s) | 172, 761, 1030, 1123 |
| HpyCH4III | ACNGT | 3 cut(s) | 395, 416, 839 |
| HpyCH4IV | ACGT | 1 cut(s) | 1019 |
| HpyCH4V | TGCA | 3 cut(s) | 504, 667, 1274 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 49, 124, 1199 |
| HpyF3I | CTNAG | 3 cut(s) | 33, 103, 1078 |
| HpySE526I | ACGT | 1 cut(s) | 1019 |
| Hsp92I | GRCGYC | 2 cut(s) | 43, 55 |
| Hsp92II | CATG | 6 cut(s) | 345, 760, 902, 914, 1071, 1248 |
| HspAI | GCGC | 1 cut(s) | 236 |
| KflI | GGGWCCC | 1 cut(s) | 358 |
| LmnI | GCTCC | 3 cut(s) | 677, 944, 1198 |
| Lsp1109I | GCAGC | 3 cut(s) | 237, 467, 1202 |
| LweI | GCATC | 1 cut(s) | 1261 |
| MaeI | CTAG | 2 cut(s) | 68, 267 |
| MaeII | ACGT | 1 cut(s) | 1019 |
| MaeIII | GTNAC | 1 cut(s) | 1289 |
| MboII | GAAGA | 7 cut(s) | 234, 241, 293, 643, 646, 656, 984 |
| MflI | RGATCY | 4 cut(s) | 93, 200, 269, 1240 |
| MlsI | TGGCCA | 1 cut(s) | 25 |
| MluCI | AATT | 5 cut(s) | 682, 800, 893, 1045, 1265 |
| MluNI | TGGCCA | 1 cut(s) | 25 |
| MlyI | GAGTC | 1 cut(s) | 1143 |
| Mox20I | TGGCCA | 1 cut(s) | 25 |
| MscI | TGGCCA | 1 cut(s) | 25 |
| MseI | TTAA | 2 cut(s) | 1041, 1297 |
| MslI | CAYNNNNRTG | 2 cut(s) | 134, 346 |
| Msp20I | TGGCCA | 1 cut(s) | 25 |
| MspA1I | CMGCKG | 1 cut(s) | 166 |
| MspI | CCGG | 2 cut(s) | 49, 675 |
| MspR9I | CCNGG | 2 cut(s) | 286, 363 |
| MvaI | CCWGG | 2 cut(s) | 286, 363 |
| MvnI | CGCG | 1 cut(s) | 256 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 49, 124, 1199 |
| NcoI | CCATGG | 2 cut(s) | 341, 1244 |
| NlaIII | CATG | 6 cut(s) | 345, 760, 902, 914, 1071, 1248 |
| NlaIV | GGNNCC | 4 cut(s) | 359, 360, 368, 946 |
| NmeAIII | GCCGAG | 1 cut(s) | 973 |
| PagI | TCATGA | 1 cut(s) | 898 |
| PasI | CCCWGGG | 1 cut(s) | 362 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 34, 224 |
| PfeI | GAWTC | 3 cut(s) | 485, 518, 981 |
| PkrI | GCNGC | 6 cut(s) | 54, 149, 252, 255, 457, 1192 |
| PleI | GAGTC | 1 cut(s) | 1142 |
| PpsI | GAGTC | 1 cut(s) | 1142 |
| Ppu21I | YACGTR | 1 cut(s) | 1020 |
| PpuMI | RGGWCCY | 2 cut(s) | 358, 367 |
| Psp5II | RGGWCCY | 2 cut(s) | 358, 367 |
| Psp6I | CCWGG | 2 cut(s) | 284, 361 |
| PspFI | CCCAGC | 3 cut(s) | 10, 166, 584 |
| PspGI | CCWGG | 2 cut(s) | 284, 361 |
| PspN4I | GGNNCC | 4 cut(s) | 359, 360, 368, 946 |
| PspPI | GGNCC | 3 cut(s) | 307, 358, 367 |
| PspPPI | RGGWCCY | 2 cut(s) | 358, 367 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 778, 810 |
| PsuI | RGATCY | 4 cut(s) | 93, 200, 269, 1240 |
| RsaI | GTAC | 2 cut(s) | 787, 1022 |
| RsaNI | GTAC | 2 cut(s) | 786, 1021 |
| RseI | CAYNNNNRTG | 2 cut(s) | 134, 346 |
| SaqAI | TTAA | 2 cut(s) | 1041, 1297 |
| SatI | GCNGC | 6 cut(s) | 53, 148, 251, 254, 456, 1191 |
| Sau96I | GGNCC | 3 cut(s) | 307, 358, 367 |
| SchI | GAGTC | 1 cut(s) | 1143 |
| ScrFI | CCNGG | 2 cut(s) | 286, 363 |
| SfaNI | GCATC | 1 cut(s) | 1261 |
| SinI | GGWCC | 2 cut(s) | 358, 367 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 134, 346 |
| SmlI | CTYRAG | 1 cut(s) | 689 |
| SmoI | CTYRAG | 1 cut(s) | 689 |
| Sse9I | AATT | 5 cut(s) | 682, 800, 893, 1045, 1265 |
| SsiI | CCGC | 5 cut(s) | 52, 147, 164, 214, 254 |
| SspI | AATATT | 1 cut(s) | 1156 |
| SspMI | CTAG | 2 cut(s) | 68, 267 |
| StyD4I | CCNGG | 2 cut(s) | 284, 361 |
| StyI | CCWWGG | 2 cut(s) | 341, 1244 |
| TaaI | ACNGT | 3 cut(s) | 395, 416, 839 |
| TaiI | ACGT | 1 cut(s) | 1022 |
| TaqI | TCGA | 5 cut(s) | 87, 227, 550, 735, 998 |
| TasI | AATT | 5 cut(s) | 682, 800, 893, 1045, 1265 |
| TatI | WGTACW | 1 cut(s) | 785 |
| TauI | GCSGC | 3 cut(s) | 55, 150, 256 |
| TfiI | GAWTC | 3 cut(s) | 485, 518, 981 |
| Tru1I | TTAA | 2 cut(s) | 1041, 1297 |
| Tru9I | TTAA | 2 cut(s) | 1041, 1297 |
| TscAI | CASTG | 1 cut(s) | 1096 |
| TseI | GCWGC | 3 cut(s) | 250, 455, 1190 |
| TspDTI | ATGAA | 6 cut(s) | 394, 415, 516, 573, 887, 906 |
| TspRI | CASTG | 1 cut(s) | 1096 |
| VpaK11BI | GGWCC | 2 cut(s) | 358, 367 |
| XapI | RAATTY | 4 cut(s) | 682, 893, 1045, 1265 |
| XbaI | TCTAGA | 1 cut(s) | 266 |
| XmiI | GTMKAC | 2 cut(s) | 660, 753 |
| XspI | CTAG | 2 cut(s) | 68, 267 |