AT5G66980

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000210
Physical Location & Seq
Forward (+)
26741543 .. 26742826
1284 bp
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UTR
Exon/CDS
Intron
AT5G66980.1

Sequence Viewer

Length: 1005 bp
ATGGTTGATCTCAGTAAAGACTTACAATTTTTCAAGGTCTTTCTCCCTGAATTCGGTTCTCACGAACTGGTGATTCCTCCTGCATTCATCGATATGTTAGAGAAACCATTACCTAAAGAAGCGTTCTTAGTCGATGAGATTGGACGGTTATGGTGTGTAGAGACTAAAACAGAAGATACAGAAGAAAGATTCTGTGTTTTCTTCACAAAAGGTTGGCAAAGTTTCGCAAACGATCAATCTCTTGAATTTGGAGACTTCCTTGTCTTCAGCTACGACGGCGATTCCAGATTCTCCGTTACGATTTTCGCTAATGACGGATGTAAAAAAGATGTTGGTGTCGTCTCAACCACGGATAGATCTAGGGTTTCTCTTGATGAGGAAGAACCGGATGATATTTTTACTAAACCGGATCGTATGAGAGATTGTGATTGTGGCCAGAGCATTAACCGGAAACGGAAACGCGATTCGGTTAATGAAGACCCTCACGTACTGGTTGATGATAAACCGGAGTATGTTTCAACTTACAAAACCAAACCGGAGCATAGTGAAAAGACTCAGAGAACAGTTAACCGGGCTGGAGATACTTGTGACATATCATGGTTTCCAGAGAAGAAACACAATGGATTTGAAGAATCGGTTTACAAACCCAAGCATCCGCATTTTGTTAGGAATATCACAAGAGGTTCACTTCAGAAATTGGAATTACCGTTAACTTTTCTGAGATCGAACGGGATTGAATTGGAGGAAGATATTGAGCTTTGTGATGAAAGTGGAAAGAAGTGGCCTTTGAAGATTCTGAATCACGACAGAGGATTCAAGTTCTCGCATGAATCATGGTTGTGTTTCTGTAAAAGCCATGAGATGATACTGACCAATAAGTGTCTATTTGAGTTTATTGTCCCAAGTAATGGGAGATGCAGTGAGATTCTAGTTCGTATTGTCAGTGGGAGATTGCCTACCACTATGACCAAAAATAATTACCAAGTTATTGATATGCAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

334

Amino Acids

38.78

Weight (kDa)

5.35

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 10 - 103 9.6e-18 B3 DNA binding domain
B3 PF02362 227 - 313 6.5e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 908
AccII CGCG 1 cut(s) 462
AciI CCGC 1 cut(s) 656
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 2 cut(s) 50, 245
AcuI CTGAAG 2 cut(s) 250, 674
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 2 cut(s) 53, 908
AgsI TTSAA 7 cut(s) 34, 245, 519, 629, 737, 790, 817
AluBI AGCT 2 cut(s) 270, 757
AluI AGCT 2 cut(s) 270, 757
Alw26I GTCTC 3 cut(s) 155, 246, 346
AlwI GGATC 1 cut(s) 417
AoxI GGCC 2 cut(s) 433, 782
ApoI RAATTY 2 cut(s) 50, 245
AsuC2I CCSGG 1 cut(s) 572
AsuHPI GGTGA 1 cut(s) 82
BalI TGGCCA 1 cut(s) 435
BbsI GAAGAC 2 cut(s) 256, 483
BceAI ACGGC 1 cut(s) 292
BcnI CCSGG 1 cut(s) 572
BcoDI GTCTC 3 cut(s) 155, 246, 346
BfaI CTAG 2 cut(s) 360, 929
BglII AGATCT 1 cut(s) 356
Bme1390I CCNGG 1 cut(s) 572
BmrFI CCNGG 1 cut(s) 572
BmsI GCATC 2 cut(s) 661, 905
BpiI GAAGAC 2 cut(s) 256, 483
BpmI CTGGAG 1 cut(s) 597
BpuMI CCSGG 1 cut(s) 572
Bsa29I ATCGAT 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 348
BsaWI WCCGGW 5 cut(s) 385, 406, 447, 505, 535
BsaXI ACNNNNNCTCC 2 cut(s) 904, 934
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 908
Bse1I ACTGG 2 cut(s) 72, 495
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 3 cut(s) 323, 394, 652
BseLI CCNNNNNNNGG 2 cut(s) 53, 908
BseMII CTCAG 3 cut(s) 25, 569, 710
BseNI ACTGG 2 cut(s) 72, 495
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 2 cut(s) 435, 784
BshVI ATCGAT 1 cut(s) 90
BsiSI CCGG 6 cut(s) 386, 407, 448, 506, 536, 571
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 2 cut(s) 53, 908
BsmAI GTCTC 3 cut(s) 155, 246, 346
BsmBI CGTCTC 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 884
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 435, 784
Bsp143I GATC 5 cut(s) 7, 232, 356, 409, 722
BspACI CCGC 1 cut(s) 656
BspANI GGCC 2 cut(s) 435, 784
BspCNI CTCAG 3 cut(s) 24, 568, 711
BspDI ATCGAT 1 cut(s) 90
BspFNI CGCG 1 cut(s) 462
BspPI GGATC 1 cut(s) 417
BsrI ACTGG 2 cut(s) 72, 495
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 5 cut(s) 7, 232, 356, 409, 722
Bst4CI ACNGT 3 cut(s) 147, 565, 708
BstBAI YACGTR 1 cut(s) 487
BstDEI CTNAG 4 cut(s) 11, 127, 555, 719
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 3 cut(s) 323, 394, 652
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 5 cut(s) 10, 235, 359, 412, 725
BstMAI GTCTC 3 cut(s) 155, 246, 346
BstMBI GATC 5 cut(s) 7, 232, 356, 409, 722
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 570
BstUI CGCG 1 cut(s) 462
BstV2I GAAGAC 2 cut(s) 256, 483
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
Bsu15I ATCGAT 1 cut(s) 90
BsuRI GGCC 2 cut(s) 435, 784
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 3 cut(s) 323, 394, 652
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 2 cut(s) 925, 949
ClaI ATCGAT 1 cut(s) 90
Csp6I GTAC 1 cut(s) 488
CviAII CATG 4 cut(s) 597, 827, 834, 857
CviJI RGCY 6 cut(s) 270, 435, 575, 757, 784, 855
CviKI_1 RGCY 6 cut(s) 270, 435, 575, 757, 784, 855
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 4 cut(s) 11, 127, 555, 719
DpnI GATC 5 cut(s) 9, 234, 358, 411, 724
DpnII GATC 5 cut(s) 7, 232, 356, 409, 722
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 250, 674
EcoRI GAATTC 1 cut(s) 50
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 4 cut(s) 600, 830, 837, 860
FaqI GGGAC 1 cut(s) 884
FatI CATG 4 cut(s) 596, 826, 833, 856
FokI GGATG 3 cut(s) 330, 401, 639
FspBI CTAG 2 cut(s) 360, 929
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 2 cut(s) 435, 784
HapII CCGG 6 cut(s) 386, 407, 448, 506, 536, 571
Hin1II CATG 4 cut(s) 600, 830, 837, 860
HincII GTYRAC 2 cut(s) 568, 711
HindII GTYRAC 2 cut(s) 568, 711
HpaI GTTAAC 2 cut(s) 568, 711
HpaII CCGG 6 cut(s) 386, 407, 448, 506, 536, 571
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 4 cut(s) 568, 640, 686, 711
Hpy188I TCNGA 4 cut(s) 558, 693, 720, 798
Hpy188III TCNNGA 6 cut(s) 62, 242, 285, 371, 605, 803
Hpy8I GTNNAC 4 cut(s) 568, 640, 686, 711
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 3 cut(s) 147, 565, 708
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 83, 918, 997
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 4 cut(s) 11, 127, 555, 719
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 4 cut(s) 600, 830, 837, 860
KspAI GTTAAC 2 cut(s) 568, 711
Kzo9I GATC 5 cut(s) 7, 232, 356, 409, 722
LmnI GCTCC 1 cut(s) 538
LweI GCATC 2 cut(s) 661, 905
MaeI CTAG 2 cut(s) 360, 929
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 295, 587
MalI GATC 5 cut(s) 9, 234, 358, 411, 724
MboI GATC 5 cut(s) 7, 232, 356, 409, 722
MflI RGATCY 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 7 cut(s) 26, 50, 245, 695, 701, 737, 976
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 1 cut(s) 547
MnlI CCTC 6 cut(s) 87, 370, 492, 674, 736, 803
Mox20I TGGCCA 1 cut(s) 435
MscI TGGCCA 1 cut(s) 435
MseI TTAA 5 cut(s) 444, 471, 567, 710, 1003
MslI CAYNNNNRTG 2 cut(s) 92, 838
Msp20I TGGCCA 1 cut(s) 435
MspI CCGG 6 cut(s) 386, 407, 448, 506, 536, 571
MspR9I CCNGG 1 cut(s) 572
Mva1269I GAATGC 1 cut(s) 83
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 276
NciI CCSGG 1 cut(s) 572
NdeII GATC 5 cut(s) 7, 232, 356, 409, 722
NlaIII CATG 4 cut(s) 600, 830, 837, 860
NmuCI GTSAC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 908
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
Ppu21I YACGTR 1 cut(s) 487
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 2 cut(s) 92, 838
SaqAI TTAA 5 cut(s) 444, 471, 567, 710, 1003
Sau3AI GATC 5 cut(s) 7, 232, 356, 409, 722
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 572
SetI ASST 8 cut(s) 39, 115, 214, 272, 489, 685, 759, 1003
SfaNI GCATC 2 cut(s) 661, 905
SmiMI CAYNNNNRTG 2 cut(s) 92, 838
Sse9I AATT 7 cut(s) 26, 50, 245, 695, 701, 737, 976
SsiI CCGC 1 cut(s) 656
SspMI CTAG 2 cut(s) 360, 929
StyD4I CCNGG 1 cut(s) 570
TaaI ACNGT 3 cut(s) 147, 565, 708
TaiI ACGT 1 cut(s) 489
TaqI TCGA 3 cut(s) 90, 132, 725
TasI AATT 7 cut(s) 26, 50, 245, 695, 701, 737, 976
Tru1I TTAA 5 cut(s) 444, 471, 567, 710, 1003
Tru9I TTAA 5 cut(s) 444, 471, 567, 710, 1003
TscAI CASTG 2 cut(s) 925, 949
TseFI GTSAC 1 cut(s) 587
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 4 cut(s) 76, 489, 780, 843
TspGWI ACGGA 4 cut(s) 283, 330, 365, 469
TspRI CASTG 2 cut(s) 925, 949
Van91I CCANNNNNTGG 1 cut(s) 908
XapI RAATTY 2 cut(s) 50, 245
XspI CTAG 2 cut(s) 360, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.