Rw6G035160

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000210
Physical Location & Seq
Reverse (-)
59743478 .. 59745067
1590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035160.1

Sequence Viewer

Length: 762 bp
ATGGCTGCTAGCAAACCAGCATTTTTCACGGTCCTCATTGGTGATTTCTGCCGGCGCCTGAAAATGCCAACTGCGTTTGTGGACAAGTTAAGGGGAACACCACCTTGCAAATGTGTTCTTAGAGGTCCCAATGGACAAAGTTGGAATGTGGCACTGGAAGAGAGAGAAAGTGGTTTGTTCTTCTGTAAGGGTTGGCGGAAATTTGTGAAGTATCATTGTTTGGAAGAAGGGGACTTTTTGGTCTTCCATTATACTGGCAAACTTAAGTTTGATGTCACAATTTATGATGAGAGTGCCTGTGAAATGGATTTAGAAGTATCCAAGAGGAGAAGTCTCGCTGATGGTAGTAGCGATCAAGTCATGTCTGGGAAGGCTGTAATTGAAGAAACACCAACTGGATTGACGGTGTTCCAATCAAGGAATTCATGTTTCATGAAAACTTTGGAGACATATAGACGTTACGATCTGACGATTCCGCAGAAAATAGTGAAAGCTGAGGGTCTTAAGGGACACATGTCTCTAACGCTTCGAGATCCAGAAAAGAGATCACGGATTGTTGAGACTGATGTCACCCTAGATAAACGTTTGAAGCTGACAAAAGGTTGGTGCCAAGGATGCAGGGAGTTCAACATCTCAATTGGGGACAAGGTTGTTTTCGAGTTTGTCAAGCCAAATGTGATTCAACTTCATATCTTCAGAGGCAAAGATGTGCTACTCTCAGGTCCTAAAGTTGTGGACTCTAGCAACTATAGGCAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

253

Amino Acids

28.87

Weight (kDa)

9.16

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 95 5.9e-16 B3 DNA binding domain
B3 PF02362 145 - 233 6.5e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccB1I GGYRCC 2 cut(s) 54, 606
AciI CCGC 2 cut(s) 196, 476
AclI AACGTT 1 cut(s) 583
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 2 cut(s) 200, 421
AcuI CTGAAG 1 cut(s) 679
AcyI GRCGYC 1 cut(s) 55
AflII CTTAAG 2 cut(s) 263, 503
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 4 cut(s) 383, 589, 628, 683
AluBI AGCT 2 cut(s) 494, 592
AluI AGCT 2 cut(s) 494, 592
Alw26I GTCTC 4 cut(s) 338, 440, 522, 554
AlwI GGATC 1 cut(s) 527
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 200, 421
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 3 cut(s) 31, 125, 722
AsuHPI GGTGA 2 cut(s) 53, 562
AsuNHI GCTAGC 1 cut(s) 8
AvaII GGWCC 3 cut(s) 31, 125, 722
BanI GGYRCC 2 cut(s) 54, 606
BbsI GAAGAC 1 cut(s) 235
BbvCI CCTCAGC 1 cut(s) 495
BccI CCATC 1 cut(s) 335
BciVI GTATCC 1 cut(s) 328
BcoDI GTCTC 4 cut(s) 338, 440, 522, 554
BfaI CTAG 4 cut(s) 9, 575, 741, 760
BfmI CTRYAG 1 cut(s) 748
BfoI RGCGCY 1 cut(s) 58
BfrI CTTAAG 2 cut(s) 263, 503
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 3 cut(s) 31, 125, 722
BmgT120I GGNCC 3 cut(s) 31, 125, 722
BmiI GGNNCC 3 cut(s) 56, 127, 608
BmsI GCATC 1 cut(s) 605
BmtI GCTAGC 1 cut(s) 12
BoxI GACNNNNGTC 2 cut(s) 514, 566
BpiI GAAGAC 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 610
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 3 cut(s) 159, 259, 400
BseDI CCNNGG 1 cut(s) 610
BseGI GGATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 486, 732
BseNI ACTGG 3 cut(s) 159, 259, 400
BseRI GAGGAG 1 cut(s) 340
BshNI GGYRCC 2 cut(s) 54, 606
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 4 cut(s) 111, 245, 522, 656
BsmAI GTCTC 4 cut(s) 338, 440, 522, 554
BsmFI GGGAC 4 cut(s) 111, 245, 522, 656
Bsp143I GATC 4 cut(s) 352, 463, 532, 545
BspACI CCGC 2 cut(s) 196, 476
BspCNI CTCAG 2 cut(s) 487, 731
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 3 cut(s) 56, 127, 608
BspOI GCTAGC 1 cut(s) 12
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 54, 606
BspTI CTTAAG 2 cut(s) 263, 503
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 3 cut(s) 159, 259, 400
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 610
BssMI GATC 4 cut(s) 352, 463, 532, 545
BssNI GRCGYC 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 31, 406
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 55
BstAFI CTTAAG 2 cut(s) 263, 503
BstC8I GCNNGC 2 cut(s) 10, 53
BstDEI CTNAG 3 cut(s) 119, 495, 718
BstF5I GGATG 1 cut(s) 620
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 4 cut(s) 355, 466, 535, 548
BstMAI GTCTC 4 cut(s) 338, 440, 522, 554
BstMBI GATC 4 cut(s) 352, 463, 532, 545
BstMWI GCNNNNNNNGC 1 cut(s) 615
BstNSI RCATGY 1 cut(s) 517
BstPAI GACNNNNGTC 2 cut(s) 514, 566
BstSFI CTRYAG 1 cut(s) 748
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 532
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 1 cut(s) 532
BsuI GTATCC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 620
BtsIMutI CAGTG 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 10, 53
CciI TCATGA 1 cut(s) 432
CfoI GCGC 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 3 cut(s) 31, 125, 722
CviAII CATG 4 cut(s) 361, 426, 433, 514
CviJI RGCY 5 cut(s) 5, 374, 494, 592, 670
CviKI_1 RGCY 5 cut(s) 5, 374, 494, 592, 670
DdeI CTNAG 3 cut(s) 119, 495, 718
DinI GGCGCC 1 cut(s) 56
DpnI GATC 4 cut(s) 354, 465, 534, 547
DpnII GATC 4 cut(s) 352, 463, 532, 545
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
EciI GGCGGA 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 610
Eco47I GGWCC 3 cut(s) 31, 125, 722
Eco57I CTGAAG 1 cut(s) 679
EcoO109I RGGNCCY 2 cut(s) 125, 722
EcoRI GAATTC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 610
EgeI GGCGCC 1 cut(s) 56
EheI GGCGCC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 610
FaeI CATG 4 cut(s) 364, 429, 436, 517
FaqI GGGAC 4 cut(s) 111, 245, 522, 656
FatI CATG 4 cut(s) 360, 425, 432, 513
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 627
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 4 cut(s) 9, 575, 741, 760
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 58
HapII CCGG 1 cut(s) 52
HhaI GCGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 55
Hin1II CATG 4 cut(s) 364, 429, 436, 517
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HinfI GANTC 3 cut(s) 472, 679, 737
HpaII CCGG 1 cut(s) 52
HphI GGTGA 2 cut(s) 53, 562
Hpy166II GTNNAC 2 cut(s) 82, 736
Hpy188I TCNGA 2 cut(s) 468, 698
Hpy188III TCNNGA 3 cut(s) 433, 530, 536
Hpy8I GTNNAC 2 cut(s) 82, 736
HpyAV CCTTC 2 cut(s) 221, 364
HpyCH4III ACNGT 2 cut(s) 31, 406
HpyCH4IV ACGT 2 cut(s) 457, 583
HpyCH4V TGCA 2 cut(s) 108, 618
HpyF10VI GCNNNNNNNGC 1 cut(s) 615
HpyF3I CTNAG 3 cut(s) 119, 495, 718
HpySE526I ACGT 2 cut(s) 457, 583
Hsp92I GRCGYC 1 cut(s) 55
Hsp92II CATG 4 cut(s) 364, 429, 436, 517
HspAI GCGC 1 cut(s) 55
KasI GGCGCC 1 cut(s) 54
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Kzo9I GATC 4 cut(s) 352, 463, 532, 545
LweI GCATC 1 cut(s) 605
MaeI CTAG 4 cut(s) 9, 575, 741, 760
MaeII ACGT 2 cut(s) 457, 583
MaeIII GTNAC 3 cut(s) 274, 458, 568
MalI GATC 4 cut(s) 354, 465, 534, 547
MboI GATC 4 cut(s) 352, 463, 532, 545
MboII GAAGA 6 cut(s) 170, 172, 235, 236, 395, 685
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 1 cut(s) 532
MluCI AATT 5 cut(s) 200, 279, 378, 421, 636
Mly113I GGCGCC 1 cut(s) 55
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 5 cut(s) 44, 116, 318, 490, 692
MroNI GCCGGC 1 cut(s) 51
MseI TTAA 3 cut(s) 89, 264, 504
MspCI CTTAAG 2 cut(s) 263, 503
MspI CCGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 636
MwoI GCNNNNNNNGC 1 cut(s) 615
NaeI GCCGGC 1 cut(s) 53
NarI GGCGCC 1 cut(s) 55
NdeII GATC 4 cut(s) 352, 463, 532, 545
NgoMIV GCCGGC 1 cut(s) 51
NheI GCTAGC 1 cut(s) 8
NlaIII CATG 4 cut(s) 364, 429, 436, 517
NlaIV GGNNCC 3 cut(s) 56, 127, 608
NmuCI GTSAC 2 cut(s) 274, 568
NspI RCATGY 1 cut(s) 517
PagI TCATGA 1 cut(s) 432
PciI ACATGT 1 cut(s) 513
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 2 cut(s) 472, 679
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 731
PluTI GGCGCC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 731
PpuMI RGGWCCY 2 cut(s) 125, 722
PscI ACATGT 1 cut(s) 513
PshAI GACNNNNGTC 2 cut(s) 514, 566
Psp1406I AACGTT 1 cut(s) 583
Psp5II RGGWCCY 2 cut(s) 125, 722
PspN4I GGNNCC 3 cut(s) 56, 127, 608
PspPI GGNCC 3 cut(s) 31, 125, 722
PspPPI RGGWCCY 2 cut(s) 125, 722
PsuI RGATCY 1 cut(s) 532
SaqAI TTAA 3 cut(s) 89, 264, 504
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 352, 463, 532, 545
Sau96I GGNCC 3 cut(s) 31, 125, 722
SchI GAGTC 1 cut(s) 731
SetI ASST 9 cut(s) 106, 127, 460, 496, 586, 594, 604, 651, 724
SfaNI GCATC 1 cut(s) 605
SfcI CTRYAG 1 cut(s) 748
SfoI GGCGCC 1 cut(s) 56
SinI GGWCC 3 cut(s) 31, 125, 722
SmlI CTYRAG 2 cut(s) 263, 503
SmoI CTYRAG 2 cut(s) 263, 503
Sse9I AATT 5 cut(s) 200, 279, 378, 421, 636
SsiI CCGC 2 cut(s) 196, 476
SspDI GGCGCC 1 cut(s) 54
SspMI CTAG 4 cut(s) 9, 575, 741, 760
StyI CCWWGG 1 cut(s) 610
TaaI ACNGT 2 cut(s) 31, 406
TaiI ACGT 2 cut(s) 460, 586
TaqI TCGA 2 cut(s) 529, 657
TasI AATT 5 cut(s) 200, 279, 378, 421, 636
TfiI GAWTC 2 cut(s) 472, 679
Tru1I TTAA 3 cut(s) 89, 264, 504
Tru9I TTAA 3 cut(s) 89, 264, 504
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 2 cut(s) 274, 568
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 274, 568
TspDTI ATGAA 4 cut(s) 414, 421, 449, 677
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 1 cut(s) 159
Vha464I CTTAAG 2 cut(s) 263, 503
VpaK11BI GGWCC 3 cut(s) 31, 125, 722
XapI RAATTY 2 cut(s) 200, 421
XceI RCATGY 1 cut(s) 517
XspI CTAG 4 cut(s) 9, 575, 741, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.