MD03G1230100.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000210
Physical Location & Seq
Reverse (-)
31532146 .. 31534423
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1230100.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGCACGGAAGGGTCCTCGGAAGAAACAATCGTTTTTCAAGGTCCTACTTGGTGACTTCTCTAAGCATCTGCGTATACCGCATGCGTTTCTCAAAAACTTAAGTGGACTATCACTCGGCAAATGCGCTCTTAAGGGCCCTAGTGGAAAACGGTGGATTGTGGAACTGGAAGAGAGAGAGAATGGCTTGTTCTTCTATGCGGCGGGTTGGCAGGATTTTGTGAAGGATCATCTTTTACAAGTGGGGGATTTTCTGGTTTTTTATTACTATGGTGAATCCAAATTTAAAGTCAAAATATACGATAAAAGTGCCTGTGAGAAGGATGTAGAGGAAGCCAACGGGAAGAGTAGCAATCCAGTTTCCTTGGAAAATTATAAAGGAAATCAAGCTAGAGTAAAAGAGGAAATTGTCGAATCTGAAGCTGAAAACTACGATACAGACTGTGAAAACAAAGCAACTATTGCTAACACAAGAAGTGGGAATGCCATGTCTGGAAAGAGACCTACAACTGGTTATGTAAAAGAAACCTCAAGCACATGCTTCACATTCAAATCGGAGAATCCATGTTTCATGGAAACTCTGAAGAAGCATCAAGAATATTGTCTGGTGATTCCAAAGAAACTAGCCATATCTGAAGGTCTTATGAGCATGGGCACTGTAATGCTTCGAAATGAATCCGGAAGAACATGGTGTGCTAAAGTTATACTTCGTCCCAAGTCATCTTTCCATCGCATGGAATTGACAACAGGTTGGGCAGGATTTTGTAACGCAAACCAGATTTCAGTTGGGGACACTGTCATTTTTGAGTTTGTTAAGCAAAGCGTAATTCAAATTCATGTTTTCAGAGCAGTGAGAGGCAAGAGGTGTGCTGTGGTACTAGATACCCTCAATGTAAAGAACGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

303

Amino Acids

34.09

Weight (kDa)

9.2

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 12 - 102 2e-16 B3 DNA binding domain
B3 PF02362 199 - 282 3.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 733
AccI GTMKAC 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 677
AciI CCGC 3 cut(s) 80, 200, 203
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 2 cut(s) 281, 831
AcuI CTGAAG 3 cut(s) 438, 602, 654
AfaI GTAC 1 cut(s) 876
AfiI CCNNNNNNNGG 2 cut(s) 509, 733
AflII CTTAAG 2 cut(s) 100, 131
AgsI TTSAA 3 cut(s) 40, 550, 830
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AluBI AGCT 2 cut(s) 389, 422
AluI AGCT 2 cut(s) 389, 422
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 1 cut(s) 234
Aor13HI TCCGGA 1 cut(s) 677
AoxI GGCC 1 cut(s) 136
ApaI GGGCCC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 281, 831
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 4 cut(s) 14, 43, 136, 137
AsuHPI GGTGA 3 cut(s) 65, 284, 619
AsuII TTCGAA 1 cut(s) 667
AvaII GGWCC 2 cut(s) 14, 43
BaeGI GKGCMC 2 cut(s) 140, 656
BanII GRGCYC 1 cut(s) 140
BccI CCATC 1 cut(s) 735
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 5 cut(s) 141, 390, 623, 878, 907
BfrI CTTAAG 2 cut(s) 100, 131
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme18I GGWCC 2 cut(s) 14, 43
BmgT120I GGNCC 4 cut(s) 14, 43, 136, 137
BmiI GGNNCC 2 cut(s) 15, 138
BmsI GCATC 2 cut(s) 76, 598
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 514
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 17, 363
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 733
Bse1I ACTGG 3 cut(s) 171, 356, 514
BseAI TCCGGA 1 cut(s) 677
BseDI CCNNGG 2 cut(s) 17, 363
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 509, 733
BseNI ACTGG 3 cut(s) 171, 356, 514
BseSI GKGCMC 2 cut(s) 140, 656
BshFI GGCC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 678
BslFI GGGAC 2 cut(s) 696, 803
BslI CCNNNNNNNGG 2 cut(s) 509, 733
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 2 cut(s) 696, 803
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 493
Bsp119I TTCGAA 1 cut(s) 667
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 140, 656
Bsp13I TCCGGA 1 cut(s) 677
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 3 cut(s) 80, 200, 203
BspANI GGCC 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 677
BspLI GGNNCC 2 cut(s) 15, 138
BspPI GGATC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 667
BspTI CTTAAG 2 cut(s) 100, 131
BspTNI GGTCTC 1 cut(s) 493
BsrI ACTGG 3 cut(s) 171, 356, 514
BssECI CCNNGG 2 cut(s) 17, 363
BssMI GATC 1 cut(s) 226
BssNAI GTATAC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 363
Bst1107I GTATAC 1 cut(s) 77
Bst4CI ACNGT 4 cut(s) 153, 443, 658, 796
Bst6I CTCTTC 2 cut(s) 165, 338
BstAFI CTTAAG 2 cut(s) 100, 131
BstAPI GCANNNNNTGC 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 667
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 328
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 79, 461
BstNSI RCATGY 2 cut(s) 86, 540
BstSLI GKGCMC 2 cut(s) 140, 656
BstZ17I GTATAC 1 cut(s) 77
BsuRI GGCC 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 713
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 3 cut(s) 654, 792, 855
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 4 cut(s) 14, 43, 136, 137
Csp6I GTAC 1 cut(s) 875
CviAII CATG 9 cut(s) 83, 487, 537, 564, 571, 649, 687, 733, 836
CviJI RGCY 7 cut(s) 138, 186, 335, 389, 422, 626, 906
CviKI_1 RGCY 7 cut(s) 138, 186, 335, 389, 422, 626, 906
CviQI GTAC 1 cut(s) 875
DdeI CTNAG 1 cut(s) 63
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
DraI TTTAAA 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 165, 338
EarI CTCTTC 2 cut(s) 165, 338
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 14, 43
Eco57I CTGAAG 3 cut(s) 438, 602, 654
EcoO109I RGGNCCY 4 cut(s) 14, 43, 136, 137
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 9 cut(s) 86, 490, 540, 567, 574, 652, 690, 736, 839
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FaqI GGGAC 2 cut(s) 696, 803
FatI CATG 9 cut(s) 82, 486, 536, 563, 570, 648, 686, 732, 835
FauI CCCGC 1 cut(s) 196
FblI GTMKAC 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 5 cut(s) 141, 390, 623, 878, 907
GlaI GCGC 1 cut(s) 127
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 678
HhaI GCGC 1 cut(s) 128
Hin1II CATG 9 cut(s) 86, 490, 540, 567, 574, 652, 690, 736, 839
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 5 cut(s) 275, 413, 559, 610, 674
HpaII CCGG 1 cut(s) 678
HphI GGTGA 3 cut(s) 65, 284, 619
Hpy166II GTNNAC 2 cut(s) 77, 107
Hpy188I TCNGA 6 cut(s) 21, 418, 556, 582, 634, 845
Hpy188III TCNNGA 3 cut(s) 492, 593, 678
Hpy8I GTNNAC 2 cut(s) 77, 107
HpyAV CCTTC 5 cut(s) 4, 217, 313, 629, 896
HpyCH4III ACNGT 4 cut(s) 153, 443, 658, 796
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 461
HpyF3I CTNAG 1 cut(s) 63
Hsp92II CATG 9 cut(s) 86, 490, 540, 567, 574, 652, 690, 736, 839
HspAI GCGC 1 cut(s) 126
Kpn2I TCCGGA 1 cut(s) 677
Kzo9I GATC 1 cut(s) 226
LweI GCATC 2 cut(s) 76, 598
MaeI CTAG 5 cut(s) 141, 390, 623, 878, 907
MaeIII GTNAC 2 cut(s) 53, 764
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 6 cut(s) 34, 182, 184, 355, 595, 693
MhlI GDGCHC 2 cut(s) 140, 656
MluCI AATT 6 cut(s) 281, 370, 405, 737, 825, 831
MnlI CCTC 7 cut(s) 27, 322, 394, 538, 848, 855, 896
MroI TCCGGA 1 cut(s) 677
MseI TTAA 4 cut(s) 101, 132, 285, 813
MslI CAYNNNNRTG 1 cut(s) 659
MspCI CTTAAG 2 cut(s) 100, 131
MspI CCGG 1 cut(s) 678
Mva1269I GAATGC 1 cut(s) 487
MwoI GCNNNNNNNGC 2 cut(s) 79, 461
NdeII GATC 1 cut(s) 226
NlaIII CATG 9 cut(s) 86, 490, 540, 567, 574, 652, 690, 736, 839
NlaIV GGNNCC 2 cut(s) 15, 138
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 53
NspI RCATGY 2 cut(s) 86, 540
NspV TTCGAA 1 cut(s) 667
PaeI GCATGC 1 cut(s) 86
PctI GAATGC 1 cut(s) 487
PfeI GAWTC 5 cut(s) 275, 413, 559, 610, 674
PflFI GACNNNGTC 1 cut(s) 794
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 1 cut(s) 202
PpuMI RGGWCCY 2 cut(s) 14, 43
PsiI TTATAA 1 cut(s) 375
Psp5II RGGWCCY 2 cut(s) 14, 43
PspN4I GGNNCC 2 cut(s) 15, 138
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 4 cut(s) 14, 43, 136, 137
PspPPI RGGWCCY 2 cut(s) 14, 43
PsyI GACNNNGTC 1 cut(s) 794
RsaI GTAC 1 cut(s) 876
RsaNI GTAC 1 cut(s) 875
RseI CAYNNNNRTG 1 cut(s) 659
SaqAI TTAA 4 cut(s) 101, 132, 285, 813
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 4 cut(s) 14, 43, 136, 137
SduI GDGCHC 2 cut(s) 140, 656
SetI ASST 8 cut(s) 45, 391, 424, 505, 530, 640, 751, 866
SfaNI GCATC 2 cut(s) 76, 598
SfuI TTCGAA 1 cut(s) 667
SinI GGWCC 2 cut(s) 14, 43
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 3 cut(s) 100, 131, 529
SmoI CTYRAG 3 cut(s) 100, 131, 529
SphI GCATGC 1 cut(s) 86
Sse9I AATT 6 cut(s) 281, 370, 405, 737, 825, 831
SsiI CCGC 3 cut(s) 80, 200, 203
SspI AATATT 1 cut(s) 599
SspMI CTAG 5 cut(s) 141, 390, 623, 878, 907
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 4 cut(s) 153, 443, 658, 796
TaqI TCGA 2 cut(s) 411, 667
TasI AATT 6 cut(s) 281, 370, 405, 737, 825, 831
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 5 cut(s) 275, 413, 559, 610, 674
Tru1I TTAA 4 cut(s) 101, 132, 285, 813
Tru9I TTAA 4 cut(s) 101, 132, 285, 813
TscAI CASTG 3 cut(s) 661, 799, 855
TseFI GTSAC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 3 cut(s) 559, 687, 824
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 3 cut(s) 661, 799, 855
Tth111I GACNNNGTC 1 cut(s) 794
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 2 cut(s) 100, 131
VpaK11BI GGWCC 2 cut(s) 14, 43
XapI RAATTY 2 cut(s) 281, 831
XceI RCATGY 2 cut(s) 86, 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 76
XspI CTAG 5 cut(s) 141, 390, 623, 878, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.