Rh1BG034700

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000210
Physical Location & Seq
Reverse (-)
4728302 .. 4731127
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG034700.1

Sequence Viewer

Length: 1179 bp
ATGGTGAAGTTGGTGAAGTTGACAACTCTTTTGCTTATGGATTCATATGGTGAAGGTAATGCAGTTCTTGAAGCCCAAGGCCCAAAAGAAAACCATCTAGCAGAGACAAGAAGCAACGGCTATAAGAAAATTAAAGCTCACTGGTTTTTCCAGTCTGCACAAAATCAAAACGAATCAGCAAGGCAGAGCAACTTTGCACAAGAAGAGGCAAGCAATATAGGAAGGACAACTGCATATAATAGTTTACAGGCCACAAAAACAGAAACCGAGCTCAGAAGTCAGACCTGCTGGTTTTATATTAAATATAAACGAGATAGAGTTATTGCTCAGCTGGGTTTCTTCTCTGTGTTCCTTACTTCTGCTGCTGCCTCAGAGTTTACACTTGAGAGAGCTCTGCATTTCCTATTCCTCTTCAAGGCTTCAAGGTACCATCTTCAAATGGCTCGTTCAGCACCAGTGCCCGCCTTTTTCCAGGTCCTTGTTGGTGATTTCTCTGAGAAACTGAAAATGCCAACAGCTTTTGTCAACGACTTCAATGGACCAGCACTATGCAAACTTCAAGGCCCTAATGGAAAATGCTGGGACGTAAAATTGGAAGAGAAAAACAATGATCTATTCTTCCATAAAGGCTGGAAGAAATTTGTGAAGGATAACTATTTGGAGGAAGGAGACTTTCTGGTTTTCGATTATGATGGCAATTCATTGTTCAGTGTGACAATCTATGATGAAAGTGCGTGTGAAATGGACATGGAAGAAGCCAAGAGAAAGAGAAGTACTGGCTGTTCTGTTGGTCAAATGAGGGATTATGTCATGCCTGGGGAGACAAGACTCAGAATCATTGAGTTCAAATCAGAGCATCTTTGCTTTAAGAGAATTATGGAGACTCACAGACTGTATCAGCTGAACATTCCTATAATAATAGCCAGGGCTAAGGGTCTCATGAGCAAGCGGACTATAGAGCTTCGGGATCCAAGCAACAGATCATGGCCTGTTACAGTGACCCCCATGGAAGATGGTCGTACACTTATGAACAAAGGCTGGTGTGATTGTTGCAAGGCGAACCAGATCAAGAAAGGGAACACCATTGTTTTTGAGTTTGTGAAGGGACATGTGAAGCTTCACATCATTAGAGATGAGGGATGTGAAGTGGTGCTCGCAGGTCCTAATATCGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

392

Amino Acids

45.0

Weight (kDa)

8.85

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 164 - 242 1.6e-16 B3 DNA binding domain
B3 PF02362 297 - 375 2.3e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 293, 1148
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 426
AciI CCGC 2 cut(s) 462, 949
AclWI GGATC 2 cut(s) 962, 975
AcsI RAATTY 1 cut(s) 638
AfaI GTAC 3 cut(s) 428, 775, 1021
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 1108
AgsI TTSAA 7 cut(s) 71, 415, 423, 437, 535, 560, 847
AjnI CCWGG 3 cut(s) 471, 814, 923
AluBI AGCT 8 cut(s) 137, 271, 331, 392, 518, 901, 961, 1117
AluI AGCT 8 cut(s) 137, 271, 331, 392, 518, 901, 961, 1117
Alw21I GWGCWC 3 cut(s) 273, 394, 1155
Alw26I GTCTC 5 cut(s) 98, 663, 815, 875, 941
AlwI GGATC 2 cut(s) 962, 975
AoxI GGCC 4 cut(s) 79, 249, 562, 986
ApeKI GCWGC 2 cut(s) 362, 365
ApoI RAATTY 1 cut(s) 638
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp718I GGTACC 1 cut(s) 426
AspS9I GGNCC 5 cut(s) 80, 475, 539, 563, 1160
AsuHPI GGTGA 4 cut(s) 16, 25, 62, 497
AvaII GGWCC 3 cut(s) 475, 539, 1160
BaeGI GKGCMC 1 cut(s) 462
BamHI GGATCC 1 cut(s) 967
BanI GGYRCC 1 cut(s) 426
BanII GRGCYC 2 cut(s) 273, 394
Bbv12I GWGCWC 3 cut(s) 273, 394, 1155
BbvI GCAGC 2 cut(s) 349, 352
BccI CCATC 4 cut(s) 102, 438, 686, 1007
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 3 cut(s) 473, 816, 925
BcoDI GTCTC 5 cut(s) 98, 663, 815, 875, 941
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 954
BfuAI ACCTGC 2 cut(s) 293, 1148
BisI GCNGC 2 cut(s) 363, 366
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 2 cut(s) 364, 367
BmcAI AGTACT 1 cut(s) 775
Bme1390I CCNGG 3 cut(s) 473, 816, 925
Bme18I GGWCC 3 cut(s) 475, 539, 1160
BmgT120I GGNCC 5 cut(s) 80, 475, 539, 563, 1160
BmiI GGNNCC 2 cut(s) 428, 969
BmrFI CCNGG 3 cut(s) 473, 816, 925
BmsI GCATC 1 cut(s) 865
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 941
BsaJI CCNNGG 4 cut(s) 76, 815, 924, 1005
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 4 cut(s) 146, 151, 455, 781
BseBI CCWGG 3 cut(s) 473, 816, 925
BseDI CCNNGG 4 cut(s) 76, 815, 924, 1005
BseGI GGATG 1 cut(s) 1145
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 5 cut(s) 286, 341, 384, 486, 844
BseNI ACTGG 4 cut(s) 146, 151, 455, 781
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 2 cut(s) 349, 352
BseYI CCCAGC 2 cut(s) 331, 579
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 4 cut(s) 81, 251, 564, 988
BshNI GGYRCC 1 cut(s) 426
BsiHKAI GWGCWC 3 cut(s) 273, 394, 1155
BslFI GGGAC 2 cut(s) 596, 1119
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 5 cut(s) 98, 663, 815, 875, 941
BsmFI GGGAC 2 cut(s) 596, 1119
BsnI GGCC 4 cut(s) 81, 251, 564, 988
Bso31I GGTCTC 1 cut(s) 941
Bsp1286I GDGCHC 4 cut(s) 273, 394, 462, 1155
Bsp143I GATC 4 cut(s) 610, 967, 980, 1065
Bsp1720I GCTNAGC 1 cut(s) 327
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 2 cut(s) 462, 949
BspANI GGCC 4 cut(s) 81, 251, 564, 988
BspCNI CTCAG 5 cut(s) 285, 340, 383, 487, 843
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 2 cut(s) 428, 969
BspMI ACCTGC 2 cut(s) 293, 1148
BspPI GGATC 2 cut(s) 962, 975
BspT107I GGYRCC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 941
BsrI ACTGG 4 cut(s) 146, 151, 455, 781
BssECI CCNNGG 4 cut(s) 76, 815, 924, 1005
BssMI GATC 4 cut(s) 610, 967, 980, 1065
BssT1I CCWWGG 2 cut(s) 76, 1005
Bst2UI CCWGG 3 cut(s) 473, 816, 925
Bst4CI ACNGT 2 cut(s) 894, 997
Bst6I CTCTTC 3 cut(s) 198, 416, 591
BstC8I GCNNGC 4 cut(s) 211, 462, 947, 1155
BstDEI CTNAG 6 cut(s) 272, 327, 370, 495, 830, 930
BstDSI CCRYGG 1 cut(s) 1005
BstENI CCTNNNNNAGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 1145
BstKTI GATC 4 cut(s) 613, 970, 983, 1068
BstMAI GTCTC 5 cut(s) 98, 663, 815, 875, 941
BstMBI GATC 4 cut(s) 610, 967, 980, 1065
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 3 cut(s) 473, 816, 925
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 3 cut(s) 471, 814, 923
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 1 cut(s) 462
BstV1I GCAGC 2 cut(s) 349, 352
BstX2I RGATCY 1 cut(s) 967
BstYI RGATCY 1 cut(s) 967
BsuRI GGCC 4 cut(s) 81, 251, 564, 988
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 1 cut(s) 1145
BtsIMutI CAGTG 4 cut(s) 139, 462, 715, 1002
BveI ACCTGC 2 cut(s) 293, 1148
Cac8I GCNNGC 4 cut(s) 211, 462, 947, 1155
CciI TCATGA 1 cut(s) 939
Cfr13I GGNCC 5 cut(s) 80, 475, 539, 563, 1160
Csp6I GTAC 3 cut(s) 427, 774, 1020
CviAII CATG 6 cut(s) 748, 811, 940, 984, 1006, 1109
CviQI GTAC 3 cut(s) 427, 774, 1020
DdeI CTNAG 6 cut(s) 272, 327, 370, 495, 830, 930
DpnI GATC 4 cut(s) 612, 969, 982, 1067
DpnII GATC 4 cut(s) 610, 967, 980, 1065
Eam1104I CTCTTC 3 cut(s) 198, 416, 591
EarI CTCTTC 3 cut(s) 198, 416, 591
Ecl136II GAGCTC 2 cut(s) 271, 392
Eco130I CCWWGG 2 cut(s) 76, 1005
Eco24I GRGCYC 2 cut(s) 273, 394
Eco31I GGTCTC 1 cut(s) 941
Eco47I GGWCC 3 cut(s) 475, 539, 1160
Eco53kI GAGCTC 2 cut(s) 271, 392
EcoICRI GAGCTC 2 cut(s) 271, 392
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoO109I RGGNCCY 3 cut(s) 475, 563, 1160
EcoRII CCWGG 3 cut(s) 471, 814, 923
EcoT14I CCWWGG 2 cut(s) 76, 1005
EcoT38I GRGCYC 2 cut(s) 273, 394
ErhI CCWWGG 2 cut(s) 76, 1005
FaeI CATG 6 cut(s) 751, 814, 943, 987, 1009, 1112
FaqI GGGAC 2 cut(s) 596, 1119
FatI CATG 6 cut(s) 747, 810, 939, 983, 1005, 1108
FauI CCCGC 1 cut(s) 469
FauNDI CATATG 1 cut(s) 46
Fnu4HI GCNGC 2 cut(s) 363, 366
FokI GGATG 1 cut(s) 1152
FriOI GRGCYC 2 cut(s) 273, 394
Fsp4HI GCNGC 2 cut(s) 363, 366
FspBI CTAG 1 cut(s) 98
GluI GCNGC 2 cut(s) 363, 366
GsaI CCCAGC 2 cut(s) 335, 583
HaeIII GGCC 4 cut(s) 81, 251, 564, 988
Hin1II CATG 6 cut(s) 751, 814, 943, 987, 1009, 1112
HincII GTYRAC 2 cut(s) 21, 526
HindII GTYRAC 2 cut(s) 21, 526
HindIII AAGCTT 1 cut(s) 1115
HinfI GANTC 5 cut(s) 41, 173, 828, 834, 883
HphI GGTGA 4 cut(s) 16, 25, 62, 497
Hpy166II GTNNAC 5 cut(s) 21, 245, 378, 526, 1022
Hpy188I TCNGA 6 cut(s) 275, 282, 373, 496, 833, 853
Hpy188III TCNNGA 4 cut(s) 68, 940, 965, 1069
Hpy8I GTNNAC 5 cut(s) 21, 245, 378, 526, 1022
HpyAV CCTTC 5 cut(s) 47, 216, 640, 659, 1096
HpyCH4III ACNGT 2 cut(s) 894, 997
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 7 cut(s) 62, 158, 197, 233, 397, 552, 1053
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 6 cut(s) 272, 327, 370, 495, 830, 930
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 6 cut(s) 751, 814, 943, 987, 1009, 1112
KpnI GGTACC 1 cut(s) 430
Kzo9I GATC 4 cut(s) 610, 967, 980, 1065
Lsp1109I GCAGC 2 cut(s) 349, 352
LweI GCATC 1 cut(s) 865
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 3 cut(s) 712, 991, 997
MalI GATC 4 cut(s) 612, 969, 982, 1067
MboI GATC 4 cut(s) 610, 967, 980, 1065
MboII GAAGA 9 cut(s) 215, 331, 403, 425, 608, 610, 646, 764, 1022
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 4 cut(s) 273, 394, 462, 1155
MluCI AATT 5 cut(s) 129, 590, 638, 697, 873
MlyI GAGTC 2 cut(s) 822, 877
MnlI CCTC 6 cut(s) 199, 379, 419, 655, 792, 1129
MseI TTAA 3 cut(s) 132, 300, 867
MspA1I CMGCKG 2 cut(s) 331, 901
MspR9I CCNGG 3 cut(s) 473, 816, 925
MvaI CCWGG 3 cut(s) 473, 816, 925
MwoI GCNNNNNNNGC 1 cut(s) 449
NcoI CCATGG 1 cut(s) 1005
NdeI CATATG 1 cut(s) 46
NdeII GATC 4 cut(s) 610, 967, 980, 1065
NlaIII CATG 6 cut(s) 751, 814, 943, 987, 1009, 1112
NlaIV GGNNCC 2 cut(s) 428, 969
NmuCI GTSAC 2 cut(s) 712, 997
NspI RCATGY 1 cut(s) 1112
PagI TCATGA 1 cut(s) 939
PciI ACATGT 1 cut(s) 1108
PfeI GAWTC 3 cut(s) 41, 173, 834
PkrI GCNGC 2 cut(s) 364, 367
PleI GAGTC 2 cut(s) 822, 877
PpsI GAGTC 2 cut(s) 822, 877
PpuMI RGGWCCY 2 cut(s) 475, 1160
PscI ACATGT 1 cut(s) 1108
Psp124BI GAGCTC 2 cut(s) 273, 394
Psp5II RGGWCCY 2 cut(s) 475, 1160
Psp6I CCWGG 3 cut(s) 471, 814, 923
PspFI CCCAGC 2 cut(s) 331, 579
PspGI CCWGG 3 cut(s) 471, 814, 923
PspN4I GGNNCC 2 cut(s) 428, 969
PspPI GGNCC 5 cut(s) 80, 475, 539, 563, 1160
PspPPI RGGWCCY 2 cut(s) 475, 1160
PsrI GAACNNNNNNTAC 2 cut(s) 766, 798
PsuI RGATCY 1 cut(s) 967
PvuII CAGCTG 2 cut(s) 331, 901
RsaI GTAC 3 cut(s) 428, 775, 1021
RsaNI GTAC 3 cut(s) 427, 774, 1020
SacI GAGCTC 2 cut(s) 273, 394
SaqAI TTAA 3 cut(s) 132, 300, 867
SatI GCNGC 2 cut(s) 363, 366
Sau3AI GATC 4 cut(s) 610, 967, 980, 1065
Sau96I GGNCC 5 cut(s) 80, 475, 539, 563, 1160
ScaI AGTACT 1 cut(s) 775
SchI GAGTC 2 cut(s) 822, 877
ScrFI CCNGG 3 cut(s) 473, 816, 925
SduI GDGCHC 4 cut(s) 273, 394, 462, 1155
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 1 cut(s) 954
SinI GGWCC 3 cut(s) 475, 539, 1160
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 5 cut(s) 129, 590, 638, 697, 873
SsiI CCGC 2 cut(s) 462, 949
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 2 cut(s) 273, 394
StyD4I CCNGG 3 cut(s) 471, 814, 923
StyI CCWWGG 2 cut(s) 76, 1005
TaaI ACNGT 2 cut(s) 894, 997
TaiI ACGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 684
TasI AATT 5 cut(s) 129, 590, 638, 697, 873
TatI WGTACW 1 cut(s) 773
TfiI GAWTC 3 cut(s) 41, 173, 834
Tru1I TTAA 3 cut(s) 132, 300, 867
Tru9I TTAA 3 cut(s) 132, 300, 867
TscAI CASTG 4 cut(s) 146, 462, 715, 1002
TseFI GTSAC 2 cut(s) 712, 997
TseI GCWGC 2 cut(s) 362, 365
Tsp45I GTSAC 2 cut(s) 712, 997
TspDTI ATGAA 4 cut(s) 33, 690, 741, 1043
TspRI CASTG 4 cut(s) 146, 462, 715, 1002
VpaK11BI GGWCC 3 cut(s) 475, 539, 1160
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 1 cut(s) 638
XceI RCATGY 1 cut(s) 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XspI CTAG 1 cut(s) 98
ZrmI AGTACT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.