MD11G1250800.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000210
Physical Location & Seq
Reverse (-)
36294578 .. 36296871
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1250800.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGCTAGAAAGCCAAAGAAACCATCTTTTATCAAGATCCTCGCCGGTGACACCTCTCAGGGTCTGCGTGTACCGCCTGCGTTTATTATGAAGAACTTCAATGGACGATCACTTTGCAAATGTGATCTTAAAGGCCCGACTGGAATTCGGAGGACTGTGGAATTGGAAGAGAGGGAGAATGGCTTGTTCTTCCATGATGGTTGGCAGGGTTTTGTGAAGGACCATCTTTTAGAAGCTGGGGATTTTTTGGTTTTTATTTATGACGGCGTATCAAAGTTTAATGTCAAAATCTATGATAGAAGTGCATGTGAGAAAGATGTCGAAGTAGCCAAGATGAATAAAGGAAATCAAGCTAGAAGAAAAGTTGAAGTTGTCGACATTGACACTGAAAACGTAAAAGCTGAAGTTGTTGACATTGACACTGTAAACTACAGCCAAGACCGTGAAAAGCAAATGATATTTTCTAGCTGGAGTTGCGATCATCTCAAATCTCGAAAGAGACCAGTAAGTAATTATGTTGAAGATACATCAACAGCATCCATCCTGTTGGAAACAGAGAATCCATGTTTTCTACACTTCTTAAACCATCGGAATCGTCTATATAATGTGAGGATTCCAAAGCAACTAGCCTTAGATGAAGGCCTCACGGACAAGGATTCCGTAACACTTCAAGATCCAGATGGAAGATCATGGTTTGTTCGACTCAGAACCCATTACACTGATCGTTTCGATTATTTGAATCTGTCAACAGGTTGGAGAGAATGCGGTAAAGCCAACCAAATTTCACTTGGGGACACCATAGTTTTTGAGTTTGTGAAGCAAAGTGTGATCCAAGTTCATATTTTCAGAAAAGGGGAAGTGAGAGGTAATGTGTGTCGTCTGGTACTTGTTGCCCCTTATGGTAAAAAGAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

306

Amino Acids

35.13

Weight (kDa)

9.12

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 100 2e-14 B3 DNA binding domain
B3 PF02362 195 - 284 1.2e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 375
AciI CCGC 2 cut(s) 74, 765
AclWI GGATC 3 cut(s) 31, 668, 823
AcsI RAATTY 2 cut(s) 144, 780
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 2 cut(s) 72, 885
AgsI TTSAA 5 cut(s) 100, 368, 521, 671, 739
AluBI AGCT 4 cut(s) 236, 353, 401, 468
AluI AGCT 4 cut(s) 236, 353, 401, 468
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 3 cut(s) 31, 668, 823
AlwNI CAGNNNCTG 1 cut(s) 64
AoxI GGCC 2 cut(s) 133, 640
ApoI RAATTY 2 cut(s) 144, 780
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
Asp700I GAANNNNTTC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 134, 220
AsuHPI GGTGA 1 cut(s) 59
AvaII GGWCC 1 cut(s) 220
BccI CCATC 6 cut(s) 31, 191, 231, 548, 594, 674
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 4 cut(s) 6, 354, 465, 626
BfmI CTRYAG 1 cut(s) 430
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 134, 220
BmsI GCATC 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 490
BsaI GGTCTC 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 145, 503
BseGI GGATG 2 cut(s) 536, 540
BseMII CTCAG 2 cut(s) 71, 718
BseNI ACTGG 2 cut(s) 145, 503
BseYI CCCAGC 1 cut(s) 236
BshFI GGCC 2 cut(s) 135, 642
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 806
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 1 cut(s) 806
BsmI GAATGC 1 cut(s) 767
BsnI GGCC 2 cut(s) 135, 642
Bso31I GGTCTC 1 cut(s) 493
Bsp143I GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
BspACI CCGC 2 cut(s) 74, 765
BspANI GGCC 2 cut(s) 135, 642
BspCNI CTCAG 2 cut(s) 70, 717
BspPI GGATC 3 cut(s) 31, 668, 823
BspTNI GGTCTC 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 2 cut(s) 145, 503
BssAI RCCGGY 1 cut(s) 44
BssMI GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
Bst4CI ACNGT 3 cut(s) 157, 424, 443
Bst6I CTCTTC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 3 cut(s) 57, 631, 704
BstF5I GGATG 2 cut(s) 536, 540
BstKTI GATC 8 cut(s) 39, 110, 127, 481, 676, 689, 724, 831
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
BstMWI GCNNNNNNNGC 2 cut(s) 73, 474
BstNSI RCATGY 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 430
BstX2I RGATCY 2 cut(s) 36, 673
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 2 cut(s) 36, 673
BsuRI GGCC 2 cut(s) 135, 642
BtsCI GGATG 2 cut(s) 536, 540
BtsIMutI CAGTG 3 cut(s) 384, 420, 717
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 64
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 2 cut(s) 134, 220
Csp6I GTAC 2 cut(s) 71, 884
CviAII CATG 4 cut(s) 194, 306, 564, 690
CviQI GTAC 2 cut(s) 71, 884
DdeI CTNAG 3 cut(s) 57, 631, 704
DpnI GATC 8 cut(s) 38, 109, 126, 480, 675, 688, 723, 830
DpnII GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 144
FaeI CATG 4 cut(s) 197, 309, 567, 693
FaqI GGGAC 1 cut(s) 806
FatI CATG 4 cut(s) 193, 305, 563, 689
FblI GTMKAC 1 cut(s) 375
FokI GGATG 2 cut(s) 523, 527
FspBI CTAG 4 cut(s) 6, 354, 465, 626
GsaI CCCAGC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 2 cut(s) 135, 642
HapII CCGG 1 cut(s) 45
Hin1II CATG 4 cut(s) 197, 309, 567, 693
HincII GTYRAC 3 cut(s) 376, 412, 747
HindII GTYRAC 3 cut(s) 376, 412, 747
HinfI GANTC 6 cut(s) 559, 592, 613, 656, 702, 739
HpaII CCGG 1 cut(s) 45
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 5 cut(s) 71, 376, 412, 427, 747
Hpy188I TCNGA 4 cut(s) 150, 591, 707, 848
Hpy188III TCNNGA 4 cut(s) 34, 492, 671, 677
Hpy8I GTNNAC 5 cut(s) 71, 376, 412, 427, 747
HpyAV CCTTC 2 cut(s) 211, 632
HpyCH4III ACNGT 3 cut(s) 157, 424, 443
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 2 cut(s) 117, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 474
HpyF3I CTNAG 3 cut(s) 57, 631, 704
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 4 cut(s) 197, 309, 567, 693
Kzo9I GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
LweI GCATC 1 cut(s) 545
MaeI CTAG 4 cut(s) 6, 354, 465, 626
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 47, 661
MalI GATC 8 cut(s) 38, 109, 126, 480, 675, 688, 723, 830
MboI GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
MboII GAAGA 6 cut(s) 103, 179, 181, 369, 533, 696
MflI RGATCY 2 cut(s) 36, 673
MluCI AATT 4 cut(s) 144, 161, 511, 780
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 528, 734
MnlI CCTC 7 cut(s) 50, 64, 144, 165, 603, 653, 857
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 3 cut(s) 129, 279, 581
MspI CCGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 73, 474
NdeII GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
NlaIII CATG 4 cut(s) 197, 309, 567, 693
NmuCI GTSAC 1 cut(s) 47
NspI RCATGY 1 cut(s) 309
PceI AGGCCT 1 cut(s) 642
PctI GAATGC 1 cut(s) 767
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 5 cut(s) 559, 592, 613, 656, 739
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PspFI CCCAGC 1 cut(s) 236
PspPI GGNCC 2 cut(s) 134, 220
PstNI CAGNNNCTG 1 cut(s) 64
PsuI RGATCY 2 cut(s) 36, 673
RsaI GTAC 2 cut(s) 72, 885
RsaNI GTAC 2 cut(s) 71, 884
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 3 cut(s) 129, 279, 581
Sau3AI GATC 8 cut(s) 36, 107, 124, 478, 673, 686, 721, 828
Sau96I GGNCC 2 cut(s) 134, 220
SchI GAGTC 1 cut(s) 696
SetI ASST 8 cut(s) 56, 238, 355, 396, 403, 470, 754, 868
SfaNI GCATC 1 cut(s) 545
SfcI CTRYAG 1 cut(s) 430
SgrAI CRCCGGYG 1 cut(s) 44
SinI GGWCC 1 cut(s) 220
Sse9I AATT 4 cut(s) 144, 161, 511, 780
SseBI AGGCCT 1 cut(s) 642
SsiI CCGC 2 cut(s) 74, 765
SspMI CTAG 4 cut(s) 6, 354, 465, 626
StuI AGGCCT 1 cut(s) 642
TaaI ACNGT 3 cut(s) 157, 424, 443
TaiI ACGT 1 cut(s) 396
TaqI TCGA 5 cut(s) 321, 375, 493, 700, 729
TasI AATT 4 cut(s) 144, 161, 511, 780
TfiI GAWTC 5 cut(s) 559, 592, 613, 656, 739
Tru1I TTAA 3 cut(s) 129, 279, 581
Tru9I TTAA 3 cut(s) 129, 279, 581
TscAI CASTG 3 cut(s) 391, 427, 724
TseFI GTSAC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 4 cut(s) 104, 350, 651, 827
TspGWI ACGGA 2 cut(s) 649, 662
TspRI CASTG 3 cut(s) 391, 427, 724
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 2 cut(s) 144, 780
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 4 cut(s) 6, 354, 465, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.