RLG00000030520

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000210
Physical Location & Seq
Reverse (-)
62860873 .. 62862042
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030520

Sequence Viewer

Length: 654 bp
ATGCCAACAGCTTTTGTCAACCACTTCAATGGATCAGCACTTTGCAAACTTCGAGGCCCTAGTGGAAAGTGCTGGGATGTAATATTGGAAGAGGAAAATAATGATATTTTCTTCCATAAAGGCTGGAATAAATTTGTGAAGGATAATTTTTTGGAGGAAGGACACATTTTGGTTTTCAATTATGATGGCAATTCTTGGTTCAATGCGACAATCTATGATAAAAGTGCATGTGAAATGGACATGGAAGCAGCCAAGACAAAGAGAAGTACTGGCGGTCTTGATGGTCAAAGGAGGGATTATGTCATGCCTGGGGAGACAAGAATCAGAATCATTGAGTTCAAAACAGAAACTATGGAGAAGCACAGACTGTATCAGCTGAGCATTCCAATAATAATAGCCAGGGCTAAGGGTCTCATAAGCAAGCGGACTATAGAGCTTCGGGATCCAAGCAACAGATCATGGCTTATTAAAGTGACTCGCATGGAAGATGGTCGTTTACTTATGAACAAAGGCTGGCGTGCTTGTAGCAAGCGGAACCAGATTAAGAAAGGGCACACCATTGTTTTTGAGTTTGTGAAGGGACATGTGAAGCTTCACATCGTTAGAGCTGAGGGGTGTGATGTGGTACTCACAGGTCCTAATGTAGTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

218

Amino Acids

24.99

Weight (kDa)

9.28

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 2 - 71 6.8e-13 B3 DNA binding domain
B3 PF02362 121 - 201 1.6e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 273, 424, 532
AclWI GGATC 3 cut(s) 40, 437, 450
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 2 cut(s) 268, 627
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 4 cut(s) 28, 178, 202, 340
AjnI CCWGG 2 cut(s) 307, 398
AluBI AGCT 5 cut(s) 11, 376, 436, 592, 608
AluI AGCT 5 cut(s) 11, 376, 436, 592, 608
Alw26I GTCTC 2 cut(s) 308, 416
AlwI GGATC 3 cut(s) 40, 437, 450
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 56, 635
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 555
BamHI GGATCC 1 cut(s) 442
BbvCI CCTCAGC 1 cut(s) 609
BbvI GCAGC 1 cut(s) 260
BccI CCATC 3 cut(s) 179, 275, 482
BciT130I CCWGG 2 cut(s) 309, 400
BcoDI GTCTC 2 cut(s) 308, 416
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 429
BisI GCNGC 1 cut(s) 249
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 2 cut(s) 309, 400
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 2 cut(s) 56, 635
BmiI GGNNCC 2 cut(s) 444, 536
BmrFI CCNGG 2 cut(s) 309, 400
Bpu10I CCTNAGC 2 cut(s) 405, 609
Bpu1102I GCTNAGC 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 308, 399
Bse1I ACTGG 1 cut(s) 274
BseBI CCWGG 2 cut(s) 309, 400
BseDI CCNNGG 2 cut(s) 308, 399
BseGI GGATG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 368, 600
BseNI ACTGG 1 cut(s) 274
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 72
BshFI GGCC 1 cut(s) 57
BslFI GGGAC 1 cut(s) 594
BsmAI GTCTC 2 cut(s) 308, 416
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 3 cut(s) 32, 442, 455
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 3 cut(s) 273, 424, 532
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 2 cut(s) 369, 601
BspLI GGNNCC 2 cut(s) 444, 536
BspPI GGATC 3 cut(s) 40, 437, 450
BspTNI GGTCTC 1 cut(s) 416
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 308, 399
BssMI GATC 3 cut(s) 32, 442, 455
Bst2UI CCWGG 2 cut(s) 309, 400
Bst4CI ACNGT 1 cut(s) 369
Bst6I CTCTTC 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 422, 515, 519, 530
BstDEI CTNAG 3 cut(s) 377, 405, 609
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 3 cut(s) 35, 445, 458
BstMAI GTCTC 2 cut(s) 308, 416
BstMBI GATC 3 cut(s) 32, 442, 455
BstNI CCWGG 2 cut(s) 309, 400
BstNSI RCATGY 2 cut(s) 231, 587
BstSCI CCNGG 2 cut(s) 307, 398
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 555
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 1 cut(s) 82
Cac8I GCNNGC 4 cut(s) 422, 515, 519, 530
Cfr13I GGNCC 2 cut(s) 56, 635
Csp6I GTAC 2 cut(s) 267, 626
CviAII CATG 6 cut(s) 228, 241, 304, 459, 481, 584
CviQI GTAC 2 cut(s) 267, 626
DdeI CTNAG 3 cut(s) 377, 405, 609
DpnI GATC 3 cut(s) 34, 444, 457
DpnII GATC 3 cut(s) 32, 442, 455
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 635
EcoO109I RGGNCCY 2 cut(s) 56, 635
EcoRII CCWGG 2 cut(s) 307, 398
FaeI CATG 6 cut(s) 231, 244, 307, 462, 484, 587
FaqI GGGAC 1 cut(s) 594
FatI CATG 6 cut(s) 227, 240, 303, 458, 480, 583
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 60
GluI GCNGC 1 cut(s) 249
GsaI CCCAGC 1 cut(s) 76
HaeIII GGCC 1 cut(s) 57
Hin1II CATG 6 cut(s) 231, 244, 307, 462, 484, 587
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 321, 327, 475
Hpy166II GTNNAC 2 cut(s) 19, 497
Hpy188I TCNGA 1 cut(s) 326
Hpy188III TCNNGA 2 cut(s) 278, 440
Hpy8I GTNNAC 2 cut(s) 19, 497
HpyAV CCTTC 3 cut(s) 133, 152, 571
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4V TGCA 2 cut(s) 45, 227
HpyF3I CTNAG 3 cut(s) 377, 405, 609
Hsp92II CATG 6 cut(s) 231, 244, 307, 462, 484, 587
Kzo9I GATC 3 cut(s) 32, 442, 455
Lsp1109I GCAGC 1 cut(s) 260
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 472
MalI GATC 3 cut(s) 34, 444, 457
MboI GATC 3 cut(s) 32, 442, 455
MboII GAAGA 3 cut(s) 101, 103, 497
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 4 cut(s) 131, 145, 178, 190
MlyI GAGTC 1 cut(s) 469
MnlI CCTC 5 cut(s) 47, 85, 148, 285, 604
MseI TTAA 2 cut(s) 468, 543
MslI CAYNNNNRTG 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 376
MspR9I CCNGG 2 cut(s) 309, 400
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 2 cut(s) 309, 400
NdeII GATC 3 cut(s) 32, 442, 455
NlaIII CATG 6 cut(s) 231, 244, 307, 462, 484, 587
NlaIV GGNNCC 2 cut(s) 444, 536
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 2 cut(s) 231, 587
PciI ACATGT 1 cut(s) 583
PctI GAATGC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 321, 327
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 635
PscI ACATGT 1 cut(s) 583
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 307, 398
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 2 cut(s) 307, 398
PspN4I GGNNCC 2 cut(s) 444, 536
PspPI GGNCC 2 cut(s) 56, 635
PspPPI RGGWCCY 1 cut(s) 635
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 268, 627
RsaNI GTAC 2 cut(s) 267, 626
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 2 cut(s) 468, 543
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 32, 442, 455
Sau96I GGNCC 2 cut(s) 56, 635
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 2 cut(s) 309, 400
SduI GDGCHC 1 cut(s) 555
SetI ASST 6 cut(s) 13, 378, 438, 594, 610, 637
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 4 cut(s) 131, 145, 178, 190
SsiI CCGC 3 cut(s) 273, 424, 532
SspI AATATT 1 cut(s) 84
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 307, 398
TaaI ACNGT 1 cut(s) 369
TaqI TCGA 1 cut(s) 52
TasI AATT 4 cut(s) 131, 145, 178, 190
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 2 cut(s) 321, 327
Tru1I TTAA 2 cut(s) 468, 543
Tru9I TTAA 2 cut(s) 468, 543
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 1 cut(s) 131
XceI RCATGY 2 cut(s) 231, 587
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.