Rorug01G0022500

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000210
Physical Location & Seq
Reverse (-)
3782484 .. 3788604
6121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0022500.1

Sequence Viewer

Length: 3144 bp
ATGATTGCAGAGAAGCCCAGTTGGATTAGGCACGAGGGTTTGCAAATTTTCTCCATTGATGTTCAGCCTGGTGGACTCAGGGTTGCCACTGGTGGGGGTGACCACAAGGTGCGGGTATGGAACATGAAATCCCTGGGCAGGGATTTCTCAAATGAAGAATCAGCCCAAAGGCTACTTGCAACCCTCCGTGATCACTTCGGTTCTGTGAATTGTGTTAGGTGGGCTAAGCATGGTCGCTATCTTGCATCAGGATCTGATGATCAAGTAATTCTAATTCATGAGAGGAAGCCAGGTTCAGGAACCACTGAGTTTGGCAGTGGAGAGCCCCCTGATGTCGAGAATTGGAAAGTTGCAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTCAATTGGTCTCCAGATGACTCGATGCTGGCCAGTGGAAGTTTAGATAACACTATACACATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGCCATTCTAGCTTGGTTAAAGGAGTTACTTGGGATCCGATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACTGTAATTATTTGGCGAACAAGTGACTGGAGTCTTGCTCACAAAACAGACGGCCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGGCGGCTTGGATGGTCCCCTTGTGGTCATTTCATTACTACCACTCATGGGTTCCAGAAACCAAGGCATTCAGCACCTGTTCTAGAGAGAGGGGAATGGTCTGCTACTTTTGACTTCTTAGGACATAATGCACCTGTGATTGTGGTGAAGTTTAACCACTCAATGTTTAAGAGGAATTTTTCCAATGCTCAAGAAGGGAAAGCTGCACCTGTTGGGTGGACCAATAGCACCTCAAAAATAGGTGGAAAAGAAAAAGAACTACAACCTTATAATGTTATAGCAATCGGGAGTCAGGATCGTACAATAACTGTATGGACGACTGCAAGTCCCCGCCCTCTCTTTGTAGCAAAGCATTTCTTCACTCAAAGTGTTGTGGATTTATCTTGGAGCCCCGATGGTTATTCACTTTTCGCATGTTCTTTGGACGGTTCAGTGGCTACTTTCCATTTTGAGGTGAAAGAACTTGGCCACCGGTTAAGTGATGCTGAACTAGATGAATTAAAGAGAAGTCGTTATGGCGATGTTAGAGGTAGGCAGGTAAATTTAGCTGAAAGCCCAGCACAGTTATTGCTTGAGGCAGCTTCTGCTAAGCAAGCCCCAAAGAAAAAAGTGGTTCGGGATGTTCAGCAAAGCCAGACAGTCAAACCTTCTGCGGATGTGGTGGTTGCAACAAAAGCATCTGATGGTCACGTTGATGATAGGAAGAAGAGTGGAGGAGCTTCTGGTGATGTTTTAAATAAAGTTTCAATGCCTGCCCGCATTTCTAGTCCTGTAAAGCAAAGAGAATATAGACGACCTGATGGTCGAAAGAGGATTATTCCAGAGGCAGTTGGTGTGCCTCTCCAGAAGGAAAATAATTCTGTTGGGGCTCAGTCCCAGGCACTTGACTTCCCTCTAATGCCTTCTGATCACAGAAAGAATGATAGTGGGTTAGTTGCTGATGATAGGGGGATTAAAGAAAATTCTGTCAGGGAAACACTTATGAGAGGCAATGATATAAAGGACGGACAGGGGGTCACCGCTAGAGCTATGATTACCAACAGCCTTGTTATTGAGAAGGTTCCCACCTCCACAGGTAGAGATGAAAGCATAAATATAGAACAGTCAGGAACTGTGAAGGCTTCTAATTCAATATGTGGTTCTAGTCCTATTCTTTCAATTAGGGTGTTTGATAAGAAGGAAGCGGAAGATGCTGTACCATATTGCTTGGAAGCTCGGCCTAAGGAACATGCTGCAAATGACATTATTAACGTGGGAAATTCACTGATCATGAAAGAAACCGAAATCACCTGCACAAAAGGGTTGCAAACACTTTGGTCTGATAGAATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGCAAACTTTTGGGCTGTTGGATGTGAAGATGGATGCATCCAGGTTTACACAGAGTGTGGGAGACGCGCTATGCCGACTATGATGGTAGGATCTGCAGCAGTTTTTATAGATTGTGACGAGTGCTGGAAGTTGTTTTTGGTTACCAGAAAAGGATCCTTGTATCTATGGGATCTATTAAAGCGGAACTGTCTCCTTCATGACTCGCTGTCATCTCTGGTTGCTTTAAACCCAAATCCATCTGCAAACGATGCAGGCATGATCAAAGTTATATCAGCAAAGCTATCAAGATCTGGATCTCCTCTTGTTGTTCTCGCTACTCGCCATGCCTTCCTTTTTGACATGGGACTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTTCTGGGTCAAATTTTGCAAGCTCCTGGCATTTGGGTTTGACGCAGAGTGGTGAGCTGGCTGGTTTGCAAGTTGATGTTAAGAAATATTTGGCTAGGAAGCCAGGTTGGAGCAGGGTGACTGACGATGGAGTACAGACACGTGCTCATTTGGAAGCTCAGCTGGCTTCTTTGCTGGCTTTAAAGTCCCCTAATGAATATCGCCAATGCCTTCTATCATATATACGCTTCCTTGCCAGAGAAGCAGATGAGTCTCGTCTACGAGAAGTCTGTGAGAGTTTCCTTGGACCTCCTACTGGAATGGTGGAAGCTACTTCTTTAGATTCAAAGAACTTGGCATGGGATCCTTTTGTGCTTGGAATGAGGAAACATAAGCTCTTACGAGAAGATATTCTCCCGGCAATGGCTTCAAATCGAAAAGTACAGCGTTTGCTCAATGAATTCATGGATCTTATTTCTGAATATGAAAGTGCTGAAATAAATGTAGAGTCAAAAACTCAAACTTCACCAACAGCACGTCTGTCAGCAGCTGATCCAATGGAGTCTGCTCCATCCAGTAAAAATAAAATGGATTCTGTCCTTGTGGCAACTGATCAAGCAAAGCCTGTTCCCGATCCAACTGATCAAAAAGACTCCACTCAGTTAGCAACAGATAGAGAAAATTCTGCTTCAACAGCAAACGATGAAGTTAATTCTGATCCACCAAAGCTCAATCACGTTACTCATGCCCCACAAAATGATGCAGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

1047

Amino Acids

114.87

Weight (kDa)

7.35

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 11 - 373 5.3e-55 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 13 - 91 7.1e-06 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 14 - 98 1e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 15 - 200 6.5e-32 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 18 - 201 2e-16 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 22 - 193 1.4e-11 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 54 - 262 7e-21 CDC20/Fizzy WD40 domain
WD40 PF00400 57 - 92 1.6e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 66 - 271 1.5e-26 THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N PF24797 68 - 230 7.8e-08 WDR35/TULP4 N-terminal
WD40_Prp19 PF24814 111 - 199 3.8e-14 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 111 - 195 8.3e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 112 - 210 2.9e-17 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 112 - 208 4.6e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 115 - 210 1.8e-09 WDR3 second beta-propeller domain
WD40 PF00400 116 - 153 5.7e-10 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 121 - 258 2.9e-15 WDHD1 first WD40 domain
Beta-prop_Aladin PF25460 122 - 232 1e-08 Aladin seven-bladed propeller
WD40 PF00400 158 - 194 2.7e-06 WD domain, G-beta repeat
Hira PF07569 691 - 890 1.2e-47 TUP1-like enhancer of split
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66980
fragaria_vesca FvH4_2g27400 FvH4_2g27400 FvH4_2g27400 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_2g40960 FvH4_3g19710 FvH4_5g30470 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_6g23740 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g01780 FvH4_7g23440 FvH4_7g23440 FvH4_7g23440
malus_domestica MD03G1230100.v1.1 MD03G1230200.v1.1 MD04G1034500.v1.1 MD04G1034600.v1.1 MD08G1039500.v1.1 MD11G1250800.v1.1 MD11G1251100.v1.1 MD11G1251200.v1.1
prunus_persica Prupe.1G388000_v2.0.a1 Prupe.1G388000_v2.0.a1 Prupe.1G388100_v2.0.a1 Prupe.4G178000_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178100_v2.0.a1 Prupe.4G178200_v2.0.a1 Prupe.4G178300_v2.0.a1 Prupe.4G178400_v2.0.a1 Prupe.4G178500_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206300_v2.0.a1 Prupe.4G206800_v2.0.a1 Prupe.4G206900_v2.0.a1 Prupe.4G207000_v2.0.a1 Prupe.7G064600_v2.0.a1 Prupe.7G064600_v2.0.a1
pyrus_communis pycom03g17770 pycom04g02860 pycom04g02870 pycom08g03160 pycom11g22190 pycom11g22200 pycom11g22210 pycom11g22220
rosa_chinensis RchiOBHm_Chr1g0318651 RchiOBHm_Chr1g0318881 RchiOBHm_Chr1g0319011 RchiOBHm_Chr1g0356381 RchiOBHm_Chr3g0482191 RchiOBHm_Chr5g0033031 RchiOBHm_Chr5g0033041 RchiOBHm_Chr6g0296251 RchiOBHm_Chr6g0299611 RchiOBHm_Chr7g0208501 RchiOBHm_Chr7g0224941
rosa_laevigata RLG00000001845 RLG00000011405 RLG00000011713 RLG00000023362 RLG00000030508 RLG00000030520 RLG00000033444
rosa_multiflora Rmu_sc0000307.1_g000013 Rmu_sc0000986.1_g000010 Rmu_sc0000986.1_g000011 Rmu_sc0002895.1_g000002 Rmu_sc0003503.1_g000005 Rmu_ssc0000357.1_g000023
rosa_roxburghii Rroxscaffold_1G00046900 Rroxscaffold_1G00046920 Rroxscaffold_1G00046930 Rroxscaffold_1G00046940 Rroxscaffold_1G00047010 Rroxscaffold_1G00047020 Rroxscaffold_1G00047030 Rroxscaffold_1G00059830 Rroxscaffold_3G00234250 Rroxscaffold_4G00299610 Rroxscaffold_4G00321350 Rroxscaffold_4G00328680 Rroxscaffold_4G00329000 Rroxscaffold_6G00399560 Rroxscaffold_6G00399970 Rroxscaffold_7G00168510 Rroxscaffold_7G00171700 Rroxscaffold_7G00178360
rosa_rugosa Rorug01G0022500 Rorug01G0022800 Rorug01G0026400 Rorug03G0195600 Rorug05G0135400 Rorug06G0260100 Rorug06G0260200.1 Rorug06G0291000 Rorug06G0291100 Rorug06G0291100 Rorug07G0227000
rosa_samantha Rh1AG035600 Rh1AG038500 Rh1AG045500 Rh1BG031400 Rh1BG034700 Rh1CG034500 Rh1CG037200 Rh1CG048500 Rh1DG032800 Rh1DG035800 Rh1DG052700 Rh3AG247000 Rh3CG277700 Rh3DG274200 Rh5AG227900 Rh5AG228000 Rh5CG256800 Rh5CG256900 Rh5CG257100 Rh5DG234000 Rh5DG234200 Rh6AG401800 Rh6BG380000 Rh6BG410200 Rh6CG385400 Rh6CG415800 Rh6DG372600 Rh6DG403000 Rh7AG376300 Rh7BG362400 Rh7DG372500
rosa_wichuraiana Rw0G008540 Rw1G002780 Rw1G002970 Rw3G022090 Rw5G020870 Rw6G032430 Rw6G035160 Rw7G031430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AarI CACCTGC 1 cut(s) 1952
AasI GACNNNNNNGTC 1 cut(s) 1455
Acc36I ACCTGC 3 cut(s) 1180, 1952, 3125
AccI GTMKAC 1 cut(s) 2650
AccII CGCG 1 cut(s) 2079
AciI CCGC 8 cut(s) 112, 658, 985, 1307, 1411, 1674, 1838, 2194
AcoI YGGCCR 3 cut(s) 420, 619, 1120
AcsI RAATTY 8 cut(s) 45, 829, 1195, 1615, 1912, 2404, 2831, 3052
AcvI CACGTG 1 cut(s) 2534
AdeI CACNNNGTG 2 cut(s) 109, 2067
AfaI GTAC 4 cut(s) 955, 1851, 2526, 2814
AfiI CCNNNNNNNGG 9 cut(s) 93, 138, 139, 296, 654, 702, 1105, 2687, 2794
AflIII ACRYGT 1 cut(s) 2531
AgeI ACCGGT 1 cut(s) 1125
AgsI TTSAA 6 cut(s) 1401, 1785, 1812, 2718, 2802, 3063
AhdI GACNNNNNGTC 3 cut(s) 978, 1667, 2218
AjiI CACGTC 1 cut(s) 2909
AjnI CCWGG 7 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494
AjuI GAANNNNNNNTTGG 2 cut(s) 2132, 2164
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 277, 309
Alw21I GWGCWC 1 cut(s) 2539
Alw26I GTCTC 4 cut(s) 405, 2068, 2207, 2649
AlwNI CAGNNNCTG 5 cut(s) 254, 731, 1238, 1766, 2921
AoxI GGCC 5 cut(s) 420, 493, 619, 1120, 1871
ApeKI GCWGC 5 cut(s) 857, 1232, 1886, 2108, 2918
ApoI RAATTY 8 cut(s) 45, 829, 1195, 1615, 1912, 2404, 2831, 3052
AsiGI ACCGGT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 2781
AspLEI GCGC 1 cut(s) 2081
AspS9I GGNCC 4 cut(s) 493, 669, 873, 2678
AsuC2I CCSGG 1 cut(s) 2789
AsuHPI GGTGA 9 cut(s) 110, 811, 1120, 1391, 1663, 1933, 2456, 2521, 2889
AvaII GGWCC 3 cut(s) 669, 873, 2678
AxyI CCTNAGG 1 cut(s) 1875
BalI TGGCCA 2 cut(s) 422, 1122
BamHI GGATCC 3 cut(s) 526, 2165, 2734
BanII GRGCYC 3 cut(s) 327, 1046, 1525
BarI GAAGNNNNNNTAC 2 cut(s) 1833, 1865
BauI CACGAG 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 2534
Bbv12I GWGCWC 1 cut(s) 2539
BbvI GCAGC 5 cut(s) 844, 1244, 1873, 2120, 2930
BccI CCATC 9 cut(s) 660, 1043, 1331, 1448, 2036, 2089, 2257, 2513, 2950
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 7 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496
BclI TGATCA 7 cut(s) 190, 259, 1561, 1920, 2271, 2983, 3013
BcnI CCSGG 1 cut(s) 2789
BcoDI GTCTC 4 cut(s) 405, 2068, 2207, 2649
BfaI CTAG 7 cut(s) 501, 737, 1145, 1419, 1677, 1797, 2487
BfmI CTRYAG 2 cut(s) 375, 2106
BfuAI ACCTGC 3 cut(s) 1180, 1952, 3125
BglII AGATCT 1 cut(s) 2300
BisI GCNGC 6 cut(s) 659, 858, 1233, 1887, 2109, 2919
BlpI GCTNAGC 3 cut(s) 225, 1242, 2550
BlsI GCNGC 6 cut(s) 660, 859, 1234, 1888, 2110, 2920
Bme1390I CCNGG 8 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496, 2789
Bme18I GGWCC 3 cut(s) 669, 873, 2678
BmeRI GACNNNNNGTC 3 cut(s) 978, 1667, 2218
BmgBI CACGTC 1 cut(s) 2909
BmgT120I GGNCC 4 cut(s) 493, 669, 873, 2678
BmrFI CCNGG 8 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496, 2789
BmrI ACTGGG 2 cut(s) 12, 634
BmsI GCATC 9 cut(s) 254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
BmuI ACTGGG 2 cut(s) 12, 634
BoxI GACNNNNGTC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 589, 621
BpmI CTGGAG 3 cut(s) 387, 616, 1481
Bpu10I CCTNAGC 1 cut(s) 2372
Bpu1102I GCTNAGC 3 cut(s) 225, 1242, 2550
BpuEI CTTGAG 2 cut(s) 828, 1247
BpuMI CCSGG 1 cut(s) 2789
BsaAI YACGTR 1 cut(s) 2534
BsaBI GATNNNNATC 1 cut(s) 2305
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 5 cut(s) 132, 133, 716, 1530, 2674
BsaWI WCCGGW 1 cut(s) 1125
Bsc4I CCNNNNNNNGG 9 cut(s) 93, 138, 139, 296, 654, 702, 1105, 2687, 2794
Bse118I RCCGGY 1 cut(s) 1125
Bse1I ACTGG 7 cut(s) 18, 94, 423, 599, 629, 2692, 2946
Bse21I CCTNAGG 1 cut(s) 1875
Bse3DI GCAATG 4 cut(s) 360, 472, 1651, 2799
Bse8I GATNNNNATC 1 cut(s) 2305
BseBI CCWGG 7 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496
BseDI CCNNGG 5 cut(s) 132, 133, 716, 1530, 2674
BseGI GGATG 7 cut(s) 671, 1279, 1315, 2039, 2049, 2051, 2942
BseJI GATNNNNATC 1 cut(s) 2305
BseLI CCNNNNNNNGG 9 cut(s) 93, 138, 139, 296, 654, 702, 1105, 2687, 2794
BseMI GCAATG 4 cut(s) 360, 472, 1651, 2799
BseMII CTCAG 5 cut(s) 91, 297, 1538, 2564, 3044
BseNI ACTGG 7 cut(s) 18, 94, 423, 599, 629, 2692, 2946
BseRI GAGGAG 2 cut(s) 1383, 2301
BseXI GCAGC 5 cut(s) 844, 1244, 1873, 2120, 2930
BseYI CCCAGC 1 cut(s) 1210
BsgI GTGCAG 2 cut(s) 843, 1930
Bsh1236I CGCG 1 cut(s) 2079
BshFI GGCC 5 cut(s) 422, 495, 621, 1122, 1873
BshTI ACCGGT 1 cut(s) 1125
BsiHKAI GWGCWC 1 cut(s) 2539
BsiSI CCGG 2 cut(s) 1126, 2789
BslFI GGGAC 5 cut(s) 655, 966, 1513, 2370, 2563
BslI CCNNNNNNNGG 9 cut(s) 93, 138, 139, 296, 654, 702, 1105, 2687, 2794
BsmAI GTCTC 4 cut(s) 405, 2068, 2207, 2649
BsmBI CGTCTC 1 cut(s) 2068
BsmFI GGGAC 5 cut(s) 655, 966, 1513, 2370, 2563
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 5 cut(s) 422, 495, 621, 1122, 1873
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 4 cut(s) 327, 1046, 1525, 2539
Bsp1720I GCTNAGC 3 cut(s) 225, 1242, 2550
BspACI CCGC 8 cut(s) 112, 658, 985, 1307, 1411, 1674, 1838, 2194
BspANI GGCC 5 cut(s) 422, 495, 621, 1122, 1873
BspCNI CTCAG 5 cut(s) 90, 298, 1537, 2563, 3043
BspFNI CGCG 1 cut(s) 2079
BspHI TCATGA 3 cut(s) 277, 1923, 2209
BspMAI CTGCAG 2 cut(s) 379, 2110
BspMI ACCTGC 3 cut(s) 1180, 1952, 3125
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 4 cut(s) 360, 472, 1651, 2799
BsrFI RCCGGY 1 cut(s) 1125
BsrI ACTGG 7 cut(s) 18, 94, 423, 599, 629, 2692, 2946
BssAI RCCGGY 1 cut(s) 1125
BssECI CCNNGG 5 cut(s) 132, 133, 716, 1530, 2674
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 2 cut(s) 716, 2674
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 7 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496
Bst4CI ACNGT 9 cut(s) 571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
Bst6I CTCTTC 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 1238, 2261
BstBAI YACGTR 1 cut(s) 2534
BstC8I GCNNGC 9 cut(s) 420, 1248, 1407, 1411, 2266, 2413, 2451, 2556, 2568
BstEII GGTNACC 3 cut(s) 98, 1669, 2152
BstENI CCTNNNNNAGG 1 cut(s) 652
BstF5I GGATG 7 cut(s) 671, 1279, 1315, 2039, 2049, 2051, 2942
BstFNI CGCG 1 cut(s) 2079
BstHHI GCGC 1 cut(s) 2081
BstMAI GTCTC 4 cut(s) 405, 2068, 2207, 2649
BstNI CCWGG 7 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496
BstNSI RCATGY 2 cut(s) 1071, 1886
BstPAI GACNNNNGTC 1 cut(s) 597
BstPI GGTNACC 3 cut(s) 98, 1669, 2152
BstSCI CCNGG 8 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494, 2787
BstSFI CTRYAG 2 cut(s) 375, 2106
BstUI CGCG 1 cut(s) 2079
BstV1I GCAGC 5 cut(s) 844, 1244, 1873, 2120, 2930
Bsu36I CCTNAGG 1 cut(s) 1875
BsuRI GGCC 5 cut(s) 422, 495, 621, 1122, 1873
BtgZI GCGATG 1 cut(s) 1188
BtrI CACGTC 1 cut(s) 2909
BtsCI GGATG 7 cut(s) 671, 1279, 1315, 2039, 2049, 2051, 2942
BtsI GCAGTG 2 cut(s) 322, 477
BtsIMutI CAGTG 9 cut(s) 87, 303, 322, 430, 477, 622, 1092, 1916, 1995
BveI ACCTGC 3 cut(s) 1180, 1952, 3125
Cac8I GCNNGC 9 cut(s) 420, 1248, 1407, 1411, 2266, 2413, 2451, 2556, 2568
CaiI CAGNNNCTG 5 cut(s) 254, 731, 1238, 1766, 2921
CciI TCATGA 3 cut(s) 277, 1923, 2209
CfoI GCGC 1 cut(s) 2081
Cfr10I RCCGGY 1 cut(s) 1125
Cfr13I GGNCC 4 cut(s) 493, 669, 873, 2678
CseI GACGC 2 cut(s) 2085, 2443
Csp6I GTAC 4 cut(s) 954, 1850, 2525, 2813
CspAI ACCGGT 1 cut(s) 1125
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviQI GTAC 4 cut(s) 954, 1850, 2525, 2813
DraI TTTAAA 3 cut(s) 1389, 2238, 2574
DraIII CACNNNGTG 2 cut(s) 109, 2067
DrdI GACNNNNNNGTC 1 cut(s) 1455
DriI GACNNNNNGTC 3 cut(s) 978, 1667, 2218
DseDI GACNNNNNNGTC 1 cut(s) 1455
EaeI YGGCCR 3 cut(s) 420, 619, 1120
Eam1104I CTCTTC 1 cut(s) 1355
Eam1105I GACNNNNNGTC 3 cut(s) 978, 1667, 2218
EarI CTCTTC 1 cut(s) 1355
Eco130I CCWWGG 2 cut(s) 716, 2674
Eco24I GRGCYC 3 cut(s) 327, 1046, 1525
Eco31I GGTCTC 1 cut(s) 405
Eco47I GGWCC 3 cut(s) 669, 873, 2678
Eco72I CACGTG 1 cut(s) 2534
Eco81I CCTNAGG 1 cut(s) 1875
Eco91I GGTNACC 3 cut(s) 98, 1669, 2152
EcoNI CCTNNNNNAGG 1 cut(s) 652
EcoO65I GGTNACC 3 cut(s) 98, 1669, 2152
EcoRI GAATTC 1 cut(s) 2831
EcoRII CCWGG 7 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494
EcoT14I CCWWGG 2 cut(s) 716, 2674
EcoT22I ATGCAT 1 cut(s) 2051
EcoT38I GRGCYC 3 cut(s) 327, 1046, 1525
ErhI CCWWGG 2 cut(s) 716, 2674
Esp3I CGTCTC 1 cut(s) 2068
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 5 cut(s) 655, 966, 1513, 2370, 2563
FauI CCCGC 3 cut(s) 105, 992, 1418
FbaI TGATCA 7 cut(s) 190, 259, 1561, 1920, 2271, 2983, 3013
FblI GTMKAC 1 cut(s) 2650
Fnu4HI GCNGC 6 cut(s) 659, 858, 1233, 1887, 2109, 2919
FokI GGATG 7 cut(s) 678, 1286, 1322, 2036, 2046, 2058, 2929
FriOI GRGCYC 3 cut(s) 327, 1046, 1525
Fsp4HI GCNGC 6 cut(s) 659, 858, 1233, 1887, 2109, 2919
FspBI CTAG 7 cut(s) 501, 737, 1145, 1419, 1677, 1797, 2487
GlaI GCGC 1 cut(s) 2080
GluI GCNGC 6 cut(s) 659, 858, 1233, 1887, 2109, 2919
GsaI CCCAGC 1 cut(s) 1214
GsuI CTGGAG 3 cut(s) 387, 616, 1481
HaeIII GGCC 5 cut(s) 422, 495, 621, 1122, 1873
HapII CCGG 2 cut(s) 1126, 2789
HgaI GACGC 2 cut(s) 2085, 2443
HhaI GCGC 1 cut(s) 2081
Hin6I GCGC 1 cut(s) 2079
HinP1I GCGC 1 cut(s) 2079
HpaII CCGG 2 cut(s) 1126, 2789
HphI GGTGA 9 cut(s) 110, 811, 1120, 1391, 1663, 1933, 2456, 2521, 2889
Hpy166II GTNNAC 4 cut(s) 74, 873, 2059, 2651
Hpy188I TCNGA 8 cut(s) 256, 531, 559, 1336, 1561, 1975, 2851, 3088
Hpy8I GTNNAC 4 cut(s) 74, 873, 2059, 2651
HpyCH4III ACNGT 9 cut(s) 571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
HpyCH4IV ACGT 5 cut(s) 1344, 1905, 2533, 2908, 3108
HpySE526I ACGT 5 cut(s) 1344, 1905, 2533, 2908, 3108
HspAI GCGC 1 cut(s) 2079
Ksp22I TGATCA 7 cut(s) 190, 259, 1561, 1920, 2271, 2983, 3013
LmnI GCTCC 6 cut(s) 542, 1041, 1370, 2420, 2502, 2944
Lsp1109I GCAGC 5 cut(s) 844, 1244, 1873, 2120, 2930
LweI GCATC 9 cut(s) 254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
MaeI CTAG 7 cut(s) 501, 737, 1145, 1419, 1677, 1797, 2487
MaeII ACGT 5 cut(s) 1344, 1905, 2533, 2908, 3108
MboII GAAGA 7 cut(s) 167, 1003, 1369, 1372, 1853, 2051, 2789
MfeI CAATTG 1 cut(s) 394
MhlI GDGCHC 4 cut(s) 327, 1046, 1525, 2539
MlsI TGGCCA 2 cut(s) 422, 1122
MluNI TGGCCA 2 cut(s) 422, 1122
MlyI GAGTC 9 cut(s) 69, 404, 607, 952, 2207, 2651, 2888, 2942, 3017
MmeI TCCRAC 3 cut(s) 2011, 2480, 3032
Mox20I TGGCCA 2 cut(s) 422, 1122
Mph1103I ATGCAT 1 cut(s) 2051
MroXI GAANNNNTTC 1 cut(s) 2781
MscI TGGCCA 2 cut(s) 422, 1122
MslI CAYNNNNRTG 1 cut(s) 1347
Msp20I TGGCCA 2 cut(s) 422, 1122
MspA1I CMGCKG 2 cut(s) 2554, 2921
MspI CCGG 2 cut(s) 1126, 2789
MspR9I CCNGG 8 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496, 2789
MunI CAATTG 1 cut(s) 394
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 7 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496
MvnI CGCG 1 cut(s) 2079
NciI CCSGG 1 cut(s) 2789
NmeAIII GCCGAG 1 cut(s) 1849
NmuCI GTSAC 7 cut(s) 98, 590, 1340, 1669, 1993, 2126, 2509
NsiI ATGCAT 1 cut(s) 2051
NspI RCATGY 2 cut(s) 1071, 1886
PagI TCATGA 3 cut(s) 277, 1923, 2209
PaqCI CACCTGC 1 cut(s) 1952
PasI CCCWGGG 1 cut(s) 133
PctI GAATGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 2781
PfeI GAWTC 3 cut(s) 158, 2714, 2963
PinAI ACCGGT 1 cut(s) 1125
PkrI GCNGC 6 cut(s) 660, 859, 1234, 1888, 2110, 2920
PleI GAGTC 9 cut(s) 69, 404, 606, 951, 2207, 2650, 2887, 2941, 3017
PmaCI CACGTG 1 cut(s) 2534
PmlI CACGTG 1 cut(s) 2534
PpsI GAGTC 9 cut(s) 69, 404, 606, 951, 2207, 2650, 2887, 2941, 3017
Ppu21I YACGTR 1 cut(s) 2534
PshAI GACNNNNGTC 1 cut(s) 597
PsiI TTATAA 1 cut(s) 924
Psp6I CCWGG 7 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494
PspCI CACGTG 1 cut(s) 2534
PspEI GGTNACC 3 cut(s) 98, 1669, 2152
PspFI CCCAGC 1 cut(s) 1210
PspGI CCWGG 7 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494
PspPI GGNCC 4 cut(s) 493, 669, 873, 2678
PstI CTGCAG 2 cut(s) 379, 2110
PstNI CAGNNNCTG 5 cut(s) 254, 731, 1238, 1766, 2921
PvuII CAGCTG 2 cut(s) 2554, 2921
RsaI GTAC 4 cut(s) 955, 1851, 2526, 2814
RsaNI GTAC 4 cut(s) 954, 1850, 2525, 2813
RseI CAYNNNNRTG 1 cut(s) 1347
SatI GCNGC 6 cut(s) 659, 858, 1233, 1887, 2109, 2919
Sau96I GGNCC 4 cut(s) 493, 669, 873, 2678
SchI GAGTC 9 cut(s) 69, 404, 607, 952, 2207, 2651, 2888, 2942, 3017
ScrFI CCNGG 8 cut(s) 69, 134, 291, 1532, 2054, 2419, 2496, 2789
SduI GDGCHC 4 cut(s) 327, 1046, 1525, 2539
SfaNI GCATC 9 cut(s) 254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
SfcI CTRYAG 2 cut(s) 375, 2106
SinI GGWCC 3 cut(s) 669, 873, 2678
SmiMI CAYNNNNRTG 1 cut(s) 1347
SmlI CTYRAG 2 cut(s) 843, 1226
SmoI CTYRAG 2 cut(s) 843, 1226
SsiI CCGC 8 cut(s) 112, 658, 985, 1307, 1411, 1674, 1838, 2194
SspI AATATT 1 cut(s) 2480
SspMI CTAG 7 cut(s) 501, 737, 1145, 1419, 1677, 1797, 2487
StyD4I CCNGG 8 cut(s) 67, 132, 289, 1530, 2052, 2417, 2494, 2787
StyI CCWWGG 2 cut(s) 716, 2674
TaaI ACNGT 9 cut(s) 571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
TaiI ACGT 5 cut(s) 1347, 1908, 2536, 2911, 3111
TaqI TCGA 4 cut(s) 336, 413, 1459, 2806
TatI WGTACW 2 cut(s) 2524, 2812
TauI GCSGC 1 cut(s) 661
TfiI GAWTC 3 cut(s) 158, 2714, 2963
TscAI CASTG 9 cut(s) 94, 310, 322, 430, 484, 629, 1092, 1923, 2002
TseFI GTSAC 7 cut(s) 98, 590, 1340, 1669, 1993, 2126, 2509
TseI GCWGC 5 cut(s) 857, 1232, 1886, 2108, 2918
Tsp45I GTSAC 7 cut(s) 98, 590, 1340, 1669, 1993, 2126, 2509
TspGWI ACGGA 2 cut(s) 176, 1674
TspRI CASTG 9 cut(s) 94, 310, 322, 430, 484, 629, 1092, 1923, 2002
VpaK11BI GGWCC 3 cut(s) 669, 873, 2678
XagI CCTNNNNNAGG 1 cut(s) 652
XapI RAATTY 8 cut(s) 45, 829, 1195, 1615, 1912, 2404, 2831, 3052
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 2 cut(s) 1071, 1886
XmiI GTMKAC 1 cut(s) 2650
XmnI GAANNNNTTC 1 cut(s) 2781
XspI CTAG 7 cut(s) 501, 737, 1145, 1419, 1677, 1797, 2487
Zsp2I ATGCAT 1 cut(s) 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.